ORCID Profile
0000-0003-0282-3716
Current Organisations
IT University of Copenhagen
,
Københavns Universitet
,
Singapore University of Social Sciences
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Publisher: SAGE Publications
Date: 05-05-2020
Abstract: Much has been written and said about the need to be agile for businesses to transform themselves and adapt in the dynamic digital economy. This case study traces the history of a Singaporean IT firm from its inception in 1993 to 2019. In particular, this case study illustrates how agility has enabled the company to grow from a start-up to be among the leading IT firms in Singapore albeit operating in the dynamic IT industry that has grown increasingly volatile with the emergence of disruptive digital technology and innovation. Apart from drawing insights on organizational agility and adaptation, this case study also provides illustration on the theme of entrepreneurship and leadership as manifested through the Founder and Chief Executive Officer.
Publisher: Wiley
Date: 29-10-2014
DOI: 10.1111/REC.12152
Publisher: Wiley
Date: 09-08-2019
DOI: 10.1111/ISJ.12215
Publisher: ACM
Date: 08-06-2016
Publisher: Wiley
Date: 30-06-2005
DOI: 10.1002/ASI.20222
Publisher: Routledge
Date: 15-05-2023
Publisher: Wiley
Date: 11-08-2022
DOI: 10.1111/TPJ.15885
Abstract: Narrow‐leafed lupin (NLL Lupinus angustifolius ) is a key rotational crop for sustainable farming systems, whose grain is high in protein content. It is a gluten‐free, non‐genetically modified, alternative protein source to soybean ( Glycine max ) and as such has gained interest as a human food ingredient. Here, we present a chromosome‐length reference genome for the species and a pan‐genome assembly comprising 55 NLL lines, including Australian and European cultivars, breeding lines and wild accessions. We present the core and variable genes for the species and report on the absence of essential mycorrhizal associated genes. The genome and pan‐genomes of NLL and its close relative white lupin ( Lupinus albus ) are compared. Furthermore, we provide additional evidence supporting LaRAP2‐7 as the key alkaloid regulatory gene for NLL and demonstrate the NLL genome is underrepresented in classical NLR disease resistance genes compared to other sequenced legume species. The NLL genomic resources generated here coupled with previously generated RNA sequencing datasets provide new opportunities to fast‐track lupin crop improvement.
Publisher: Springer Science and Business Media LLC
Date: 12-2021
DOI: 10.1186/S13007-021-00832-4
Abstract: Lupins are promising protein crops with an increasing amount of genomic and transcriptomic resources. The new resources facilitate the in silico identification of candidate genes controlling important agronomic traits. However, a major bottleneck for lupin research and crop improvement is the in planta characterization of gene function. Here, we present an efficient protocol for virus-induced gene silencing (VIGS) to down-regulate endogenous genes in narrow-leafed lupin (NLL) using the apple latent spherical virus (ALSV). We identified ALSV as an appropriate VIGS vector able to infect NLL without causing a discernible phenotype. We created improved ALSV vectors to allow for efficient cloning of gene fragments into the viral genome and for easier viral propagation via agroinfiltration of Nicotiana benthamiana . Using this system, we silenced the visual marker gene phytoene desaturase ( PDS ), which resulted in systemic, homogenous silencing as indicated by bleaching of newly produced tissues. Furthermore, by silencing lysine decarboxylase ( LaLDC )—a gene likely to be involved in toxic alkaloid biosynthesis—we demonstrate the applicability of our VIGS method to silence a target gene alone or alongside PDS in a ‘PDS co-silencing’ approach. The co-silencing approach allows the visual identification of tissues where silencing is actively occurring, which eases tissue harvesting and downstream analysis, and is useful where the trait under study is not affected by PDS silencing. Silencing LaLDC resulted in a ~ 61% or ~ 67% decrease in transcript level, depending on whether LaLDC was silenced alone or alongside PDS . Overall, the silencing of LaLDC resulted in reduced alkaloid levels, providing direct evidence of its involvement in alkaloid biosynthesis in NLL. We provide a rapid and efficient VIGS method for validating gene function in NLL. This will accelerate the research and improvement of this underutilized crop.
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Calvin M.L. Chan.