ORCID Profile
0000-0002-5462-5861
Current Organisation
CSIRO
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Publisher: Oxford University Press (OUP)
Date: 08-02-2016
DOI: 10.1093/JXB/ERV555
Publisher: Oxford University Press (OUP)
Date: 04-10-2016
DOI: 10.1093/JXB/ERW357
Publisher: Oxford University Press (OUP)
Date: 17-07-2018
DOI: 10.1104/PP.18.00618
Publisher: The Company of Biologists
Date: 15-07-2007
DOI: 10.1242/DEV.02864
Abstract: Carpels and leaves are evolutionarily related organs, as the former are thought to be modified leaves. Therefore, developmental pathways that play crucial roles in patterning both organs are presumably conserved. In leaf primordia of Arabidopsis thaliana, the ASYMMETRIC LEAVES1(AS1) gene interacts with AS2 to repress the class I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS(BP), KNAT2 and KNAT6, restricting the expression of these genes to the meristem. In this report, we describe how AS1,presumably in collaboration with AS2, patterns the Arabidopsis gynoecium by repressing BP, which is expressed in the replum and valve margin, interacts in the replum with REPLUMLESS (RPL), an essential gene for replum development,and positively regulates the expression of this gene. Misexpression of BP in the gynoecium causes an increase in replum size, while the valve width is slightly reduced, and enhances the effect of mutations in FRUITFULL (FUL), a gene with an important function in valve development. Altogether, these findings strongly suggest that BPplays a crucial role in replum development. We propose a model for pattern formation along the mediolateral axis of the ovary, whereby three domains(replum, valve margin and valve) are specified by the opposing gradients of two antagonistic factors, valve factors and replum factors, the class I KNOX genes working as the latter.
Publisher: Public Library of Science (PLoS)
Date: 22-06-2017
Publisher: Elsevier BV
Date: 09-2009
DOI: 10.1016/J.YDBIO.2009.06.035
Abstract: Plant floral transition is a major developmental switch regulated by an integrated network of pathways. Arabidopsis FLOWERING LOCUS K (FLK), a protein with three KH RNA-binding domains, operates in the autonomous flowering-promotive pathway by decreasing the transcript levels of the key flowering repressor FLOWERING LOCUS C (FLC). Here we report that PEPPER (PEP), an FLK paralog previously shown to affect vegetative and pistil development, antagonizes FLK by positively regulating FLC. Lack of PEP function rescues the flk late-flowering phenotype with a concomitant decrease in FLC RNA levels. Loss of HUA2, another FLC activator encoding an RNA-binding protein, further rescues flk, being flk hua2 pep triple mutants virtually wild-type regarding flowering time. Consistently, PEP overexpression determines high levels of FLC transcripts and flowering delay. Genetic and molecular analyses indicate that FLK and PEP act independently of FCA, another important FLC repressor in the autonomous pathway. In addition, we present data suggesting that PEP may affect FLC expression at both transcriptional and post-transcriptional levels. Overall, our results uncover PEP as a new factor for FLC upregulation, underscoring the importance of RNA-binding activities during developmental timing of flowering.
Publisher: eLife Sciences Publications, Ltd
Date: 29-11-2021
DOI: 10.7554/ELIFE.70095
Abstract: A fundamental limitation of photosynthetic carbon fixation is the availability of CO 2 . In C 4 plants, primary carboxylation occurs in mesophyll cytosol, and little is known about the role of CO 2 diffusion in facilitating C 4 photosynthesis. We have examined the expression, localization, and functional role of selected plasma membrane intrinsic aquaporins (PIPs) from Setaria italica (foxtail millet) and discovered that SiPIP2 is CO 2 -permeable. When ectopically expressed in mesophyll cells of Setaria viridis (green foxtail), SiPIP2 was localized to the plasma membrane and caused no marked changes in leaf biochemistry. Gas exchange and C 18 O 16 O discrimination measurements revealed that targeted expression of SiPIP2 enhanced the conductance to CO 2 diffusion from the intercellular airspace to the mesophyll cytosol. Our results demonstrate that mesophyll conductance limits C 4 photosynthesis at low p CO 2 and that SiPIP2 is a functional CO 2 permeable aquaporin that can improve CO 2 diffusion at the airspace/mesophyll interface and enhance C 4 photosynthesis.
Publisher: eLife Sciences Publications, Ltd
Date: 17-10-2021
Publisher: Springer Science and Business Media LLC
Date: 11-09-2009
Abstract: Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. Indeed, nearly 40% of genes in plants encode proteins of unknown function. Functional characterization of these genes is one of the main challenges in modern biology. In this regard, the availability of full-length cDNA clones may fill in the gap created between sequence information and biological knowledge. Full-length cDNA clones facilitate functional analysis of the corresponding genes enabling manipulation of their expression in heterologous systems and the generation of a variety of tagged versions of the native protein. In addition, the development of full-length cDNA sequences has the power to improve the quality of genome annotation. We developed an integrated method to generate a new normalized EST collection enriched in full-length and rare transcripts of different citrus species from multiple tissues and developmental stages. We constructed a total of 15 cDNA libraries, from which we isolated 10,898 high-quality ESTs representing 6142 different genes. Percentages of redundancy and proportion of full-length clones range from 8 to 33, and 67 to 85, respectively, indicating good efficiency of the approach employed. The new EST collection adds 2113 new citrus ESTs, representing 1831 unigenes, to the collection of citrus genes available in the public databases. To facilitate functional analysis, cDNAs were introduced in a Gateway-based cloning vector for high-throughput functional analysis of genes in planta . Herein, we describe the technical methods used in the library construction, sequence analysis of clones and the overexpression of CitrSEP , a citrus homolog to the Arabidopsis SEP3 gene, in Arabidopsis as an ex le of a practical application of the engineered Gateway vector for functional analysis. The new EST collection denotes an important step towards the identification of all genes in the citrus genome. Furthermore, public availability of the cDNA clones generated in this study, and not only their sequence, enables testing of the biological function of the genes represented in the collection. Expression of the citrus SEP3 homologue, CitrSEP , in Arabidopsis results in early flowering, along with other phenotypes resembling the over-expression of the Arabidopsis SEPALLATA genes. Our findings suggest that the members of the SEP gene family play similar roles in these quite distant plant species.
Publisher: Wiley
Date: 27-10-2020
DOI: 10.1111/PBI.13487
Location: Australia
No related grants have been discovered for Hugo Alonso-Cantabrana.