Helen Bothwell

Multi‐scale habitat modelling identifies spatial conservation priorities for mainland clouded leopards (Neofelis nebulosa)

Publisher: Wiley

Date: 18-07-2019

DOI: 10.1111/DDI.12967

Regarding the F‐word: The effects of data filtering on inferred genotype‐environment associations

Publisher: Wiley

Date: 09-03-2021

DOI: 10.1111/1755-0998.13351

Abstract: Genotype‐environment association (GEA) methods have become part of the standard landscape genomics toolkit, yet, we know little about how to best filter genotype‐by‐sequencing data to provide robust inferences for environmental adaptation. In many cases, default filtering thresholds for minor allele frequency and missing data are applied regardless of s le size, having unknown impacts on the results, negatively affecting management strategies. Here, we investigate the effects of filtering on GEA results and the potential implications for assessment of adaptation to environment. We use empirical and simulated data sets derived from two widespread tree species to assess the effects of filtering on GEA outputs. Critically, we find that the level of filtering of missing data and minor allele frequency affect the identification of true positives. Even slight adjustments to these thresholds can change the rate of true positive detection. Using conservative thresholds for missing data and minor allele frequency substantially reduces the size of the data set, lessening the power to detect adaptive variants (i.e., simulated true positives) with strong and weak strengths of selection. Regardless, strength of selection was a good predictor for GEA detection, but even some SNPs under strong selection went undetected. False positive rates varied depending on the species and GEA method, and filtering significantly impacted the predictions of adaptive capacity in downstream analyses. We make several recommendations regarding filtering for GEA methods. Ultimately, there is no filtering panacea, but some choices are better than others, depending on the study system, availability of genomic resources, and desired objectives.

Landscape drivers of genomic diversity and divergence in woodland Eucalyptus

Publisher: Wiley

Date: 17-11-2019

DOI: 10.1111/MEC.15287

Exploring polymorphic interspecies structural variants in Eucalyptus: Unravelling Their Role in Reproductive Isolation and Adaptive Divergence

Publisher: Cold Spring Harbor Laboratory

Date: 21-10-2023

DOI: 10.1101/2023.10.20.563207

University Of Georgia

Location: United States of America

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Discovery Early Career Researcher Award - Grant ID: DE190100326

Start Date: 06-2019

End Date: 05-2022

Amount: $419,406.00

Funder: Australian Research Council