ORCID Profile
0000-0002-5589-5548
Current Organisation
Bundesanstalt für Materialforschung und -prüfung
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Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0AN00319K
Abstract: The employment of type-I pyrethroids for airplane disinfection in recent years underlines the necessity to develop sensing schemes for the rapid detection of these pesticides directly at the point-of-use.
Publisher: Wiley
Date: 05-2008
Abstract: Acoustic levitation is used as a new tool to study concentration-dependent processes in fluorescence spectroscopy. With this technique, small amounts of liquid and solid s les can be measured without the need for s le supports or containers, which often limits signal acquisition and can even alter s le properties due to interactions with the support material. We demonstrate that, because of the small s le volume, fluorescence measurements at high concentrations of an organic dye are possible without the limitation of inner-filter effects, which h er such experiments in conventional, cuvette-based measurements. Furthermore, we show that acoustic levitation of liquid s les provides an experimentally simple way to study distance-dependent fluorescence modulations in semiconductor nanocrystals. The evaporation of the solvent during levitation leads to a continuous increase of solute concentration and can easily be monitored by laser-induced fluorescence.
Publisher: MDPI AG
Date: 08-04-2022
Abstract: Sialic acid (SA) is a monosaccharide usually linked to the terminus of glycan chains on the cell surface. It plays a crucial role in many biological processes, and hypersialylation is a common feature in cancer. Lectins are widely used to analyze the cell surface expression of SA. However, these protein molecules are usually expensive and easily denatured, which calls for the development of alternative glycan-specific receptors and cell imaging technologies. In this study, SA-imprinted fluorescent core-shell molecularly imprinted polymer particles (SA-MIPs) were employed to recognize SA on the cell surface of cancer cell lines. The SA-MIPs improved suspensibility and scattering properties compared with previously used core-shell SA-MIPs. Although SA-imprinting was performed using SA without preference for the α2,3- and α2,6-SA forms, we screened the cancer cell lines analyzed using the lectins Maackia Amurensis Lectin I (MAL I, α2,3-SA) and Sambucus Nigra Lectin (SNA, α2,6-SA). Our results show that the selected cancer cell lines in this study presented a varied binding behavior with the SA-MIPs. The binding pattern of the lectins was also demonstrated. Moreover, two different pentavalent SA conjugates were used to inhibit the binding of the SA-MIPs to breast, skin, and lung cancer cell lines, demonstrating the specificity of the SA-MIPs in both flow cytometry and confocal fluorescence microscopy. We concluded that the synthesized SA-MIPs might be a powerful future tool in the diagnostic analysis of various cancer cells.
Location: Germany
No related grants have been discovered for Knut Rurack.