ORCID Profile
0000-0001-7813-834X
Current Organisation
Queensland Government
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Forestry Pests, Health and Diseases | Forestry Sciences | Horticultural Crop Improvement (Selection and Breeding) | Conservation and Biodiversity |
Essential Oil Crops (e.g. Tea Tree, Eucalyptus, Lavender, Peppermint, Boronia, Sandalwood) | Forest and Woodlands Flora, Fauna and Biodiversity
Publisher: MDPI AG
Date: 2018
Publisher: Springer Science and Business Media LLC
Date: 13-10-2016
Publisher: Future Science Ltd
Date: 11-2018
Abstract: It is challenging to sequence and assemble genomes of obligate plant pathogens and microorganisms because of limited amounts of DNA, comparatively large genomes and high numbers of repeat regions. We sequenced the 1.2 gigabase genome of an obligate rust fungus, Austropuccinia psidii, the cause of rust on Myrtaceae, with a Chromium 10X library. This technology has mostly been applied for single-cell sequencing in immunological studies of mammals. We compared scaffolds of a genome assembled from the Chromium library with one assembled from combined paired-end and mate-pair libraries, sequenced with Illumina HiSeq. Chromium 10X provided a superior assembly, in terms of number of scaffolds, N50 and number of genes recovered. It required less DNA than other methods and was sequenced and assembled at a lower cost. Chromium sequencing could provide a solution to sequence and assemble genomes of obligate plant pathogens where the amount of available DNA is a limiting factor.
Publisher: Public Library of Science (PLoS)
Date: 05-05-2016
Publisher: Scientific Societies
Date: 06-2023
DOI: 10.1094/PHYTO-07-22-0257-R
Abstract: Austropuccinia psidii, commonly known as myrtle rust, is an obligate, biotrophic rust pathogen that causes rust disease in a broad host range of Myrtaceae species. Eucalyptus grandis, a widely cultivated hardwood Myrtaceae species, is susceptible to A. psidii infection, with this pathogen threatening both their natural range and various forest plantations across the world. This study aimed to investigate the A. psidii transcriptomic responses in resistant and susceptible E. grandis at four time points. RNA-seq reads were mapped to the A. psidii reference genome to quantify expressed genes at 12 h postinoculation and 1, 2, and 5 days postinoculation (dpi). A total of eight hundred and ninety expressed genes were found, of which 43 were candidate effector protein genes. These included rust transferred protein 1 ( RTP1), expressed in susceptible hosts at 5 dpi, and a hydrolase protein gene expressed in both resistant and susceptible hosts over time. Functional categorization of expressed genes revealed processes enriched in susceptible hosts, including malate metabolic and malate dehydrogenase activity, implicating oxalic acid in disease susceptibility. These results highlight putative virulence or pathogenicity mechanisms employed by A. psidii to cause disease, and they provide the first insight into the molecular responses of A. psidii in E. grandis over time.
Publisher: Frontiers Media SA
Date: 02-03-2016
Publisher: Wiley
Date: 09-06-2011
Publisher: Wiley
Date: 17-12-2022
DOI: 10.1111/MPP.13286
Abstract: Rust fungi (Pucciniales) are a erse group of plant pathogens in natural and agricultural systems. They pose ongoing threats to the ersity of native flora and cause annual crop yield losses. Agricultural rusts are predominantly managed with fungicides and breeding for resistance, but new control strategies are needed on non‐agricultural plants and in fragile ecosystems. RNA interference (RNAi) induced by exogenous double‐stranded RNA (dsRNA) has promise as a sustainable approach for managing plant‐pathogenic fungi, including rust fungi. We investigated the mechanisms and impact of exogenous dsRNA on rust fungi through in vitro and whole‐plant assays using two species as models, Austropuccinia psidii (the cause of myrtle rust) and Coleosporium plumeriae (the cause of frangipani rust) . In vitro, dsRNA either associates externally or is internalized by urediniospores during the early stages of germination. The impact of dsRNA on rust infection architecture was examined on artificial leaf surfaces. dsRNA targeting predicted essential genes significantly reduced germination and inhibited development of infection structures, namely appressoria and penetration pegs. Exogenous dsRNA sprayed onto 1‐year‐old trees significantly reduced myrtle rust symptoms . Furthermore, we used comparative genomics to assess the wide‐scale amenability of dsRNA to control rust fungi. We sequenced genomes of six species of rust fungi, including three new families (Araucariomyceaceae, Phragmidiaceae, and Skierkaceae) and identified key genes of the RNAi pathway across 15 species in eight families of Pucciniales. Together, these findings indicate that dsRNA targeting essential genes has potential for broad‐use management of rust fungi across natural and agricultural systems.
Publisher: Springer Science and Business Media LLC
Date: 27-01-2011
Publisher: Scientific Societies
Date: 08-2015
Publisher: Wiley
Date: 30-11-2020
DOI: 10.1111/PPA.13321
Publisher: Wiley
Date: 28-01-2020
DOI: 10.1111/PPA.13145
Publisher: American Society for Microbiology
Date: 15-02-2018
Abstract: The complete genome sequence of Cucumber mosaic virus strain K was determined by deep RNA sequencing. The tripartite genome consists of a 3,382-nucleotide (nt) RNA1, a 3,050-nt RNA2, and a 2,218-nt RNA3 segment. Phylogenetic analysis placed RNA1 and RNA2 in subgroup IB. However, RNA3 grouped with subgroup IA isolates, indicating a likely recombination event.
Publisher: Wiley
Date: 29-09-2017
DOI: 10.1111/PPA.12763
Publisher: Wiley
Date: 14-08-2015
Publisher: International Society for Horticultural Science (ISHS)
Date: 07-2016
Publisher: National Inquiry Services Center (NISC)
Date: 21-06-2019
Publisher: Springer Science and Business Media LLC
Date: 19-02-2022
DOI: 10.1007/S10658-022-02480-3
Abstract: Quambalaria are fungal pathogens of Corymbia , Eucalyptus and related genera of Myrtaceae. They are smut fungi (Ustilaginomycota) described from structures that resemble conidia and conidiophores. Whether these spore forms have asexual or sexual roles in life cycles of Quambalaria is unknown. An epidemic of Q. pitereka destroyed plantations of Corymbia in New South Wales and Queensland (Australia) in 2008. We s led 177 in iduals from three plantations of C. variegata and used AFLPs to test hypotheses that the epidemic was spread by asexual reproduction and dominated by a single genotype. There was high genotypic ersity across ≥600 AFLP loci in the pathogen populations at each plantation, and evidence of sexual reproduction based on neighbour-net analyses and rejection of linkage disequilibrium. The populations were not structured by host or location. Our data did not support a hypothesis of asexual reproduction but instead that Q. pitereka spreads exclusively by sexual reproduction, similar to life cycles of other smut fungi. Epidemics were exacerbated by monocultures of Corymbia established from seed collected from a single provenance. This study showcases an ex le of an endemic pathogen, Q. pitereka , with a strictly outbreeding life cycle that has caused epidemics when susceptible hosts were planted in large monoculture plantations.
Publisher: Springer Science and Business Media LLC
Date: 27-09-2014
Start Date: 09-2020
End Date: 12-2023
Amount: $390,000.00
Funder: Australian Research Council
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