ORCID Profile
0000-0002-8547-5309
Current Organisations
Agricultural Genomic Institute at Shenzhen, Chinese Academy of Agricultural Sciences
,
Chinese Academy of Tropical Agricultural Sciences
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Publications
Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors
Publisher: Springer Science and Business Media LLC
Date: 28-08-2023
DOI: 10.1038/S41588-023-01486-9
Abstract: Potato ( Solanum tuberosum ) and tomato ( Solanum lycopersicon ) crops suffer severe losses to late blight caused by the oomycete pathogen Phytophthora infestans . Solanum americanum , a relative of potato and tomato, is globally distributed and most accessions are highly blight resistant. We generated high-quality reference genomes of four S. americanum accessions, resequenced 52 accessions, and defined a pan-NLRome of S. americanum immune receptor genes. We further screened for variation in recognition of 315 P. infestans RXLR effectors in 52 S. americanum accessions. Using these genomic and phenotypic data, we cloned three NLR-encoding genes, Rpi-amr4 , R02860 and R04373 , that recognize cognate P. infestans RXLR effectors PITG_22825 ( AVRamr4 ), PITG_02860 and PITG_04373 . These genomic resources and methodologies will support efforts to engineer potatoes with durable late blight resistance and can be applied to diseases of other crops.
A nonS-locus F-box gene breaks self-incompatibility in diploid potatoes
Publisher: Springer Science and Business Media LLC
Date: 06-07-2021
DOI: 10.1038/S41467-021-24266-7
Abstract: Potato is the third most important staple food crop. To address challenges associated with global food security, a hybrid potato breeding system, aimed at converting potato from a tuber-propagated tetraploid crop into a seed-propagated diploid crop through crossing inbred lines, is under development. However, given that most diploid potatoes are self-incompatible, this represents a major obstacle which needs to be addressed in order to develop inbred lines. Here, we report on a self-compatible diploid potato, RH89-039-16 (RH), which can efficiently induce a mating transition from self-incompatibility to self-compatibility, when crossed to self-incompatible lines. We identify the S - l ocus i nhibitor ( Sli ) gene in RH, capable of interacting with multiple allelic variants of the pistil-specific S-ribonucleases (S-RNases). Further, Sli gene functions like a general S-RNase inhibitor, to impart SC to RH and other self-incompatible potatoes. Discovery of Sli now offers a path forward for the diploid hybrid breeding program.
The wax gourd genomes offer insights into the genetic diversity and ancestral cucurbit karyotype
Publisher: Springer Science and Business Media LLC
Date: 14-11-2019
DOI: 10.1038/S41467-019-13185-3
Abstract: The botanical family Cucurbitaceae includes a variety of fruit crops with global or local economic importance. How their genomes evolve and the genetic basis of ersity remain largely unexplored. In this study, we sequence the genome of the wax gourd ( Benincasa hispida ), which bears giant fruit up to 80 cm in length and weighing over 20 kg. Comparative analyses of six cucurbit genomes reveal that the wax gourd genome represents the most ancestral karyotype, with the predicted ancestral genome having 15 proto-chromosomes. We also resequence 146 lines of erse germplasm and build a variation map consisting of 16 million variations. Combining population genetics and linkage mapping, we identify a number of regions/genes potentially selected during domestication and improvement, some of which likely contribute to the large fruit size in wax gourds. Our analyses of these data help to understand genome evolution and function in cucurbits.
The tomato genome sequence provides insights into fleshy fruit evolution
Publisher: Springer Science and Business Media LLC
Date: 05-2012
DOI: 10.1038/NATURE11119
The genome of the mesopolyploid crop species Brassica rapa
Publisher: Springer Science and Business Media LLC
Date: 28-08-2011
DOI: 10.1038/NG.919
Abstract: We report the annotation and analysis of the draft genome sequence of Brassica rapa accession Chiifu-401-42, a Chinese cabbage. We modeled 41,174 protein coding genes in the B. rapa genome, which has undergone genome triplication. We used Arabidopsis thaliana as an outgroup for investigating the consequences of genome triplication, such as structural and functional evolution. The extent of gene loss (fractionation) among triplicated genome segments varies, with one of the three copies consistently retaining a disproportionately large fraction of the genes expected to have been present in its ancestor. Variation in the number of members of gene families present in the genome may contribute to the remarkable morphological plasticity of Brassica species. The B. rapa genome sequence provides an important resource for studying the evolution of polyploid genomes and underpins the genetic improvement of Brassica oil and vegetable crops.
Gain-of-function of the 1-aminocyclopropane-1-carboxylate synthase geneACS1Ginduces female flower development in cucumber gynoecy
Publisher: Oxford University Press (OUP)
Date: 02-12-2020
Abstract: Unisexual flowers provide a useful system for studying plant sex determination. In cucumber (Cucumis sativus L.), three major Mendelian loci control unisexual flower development, Female (F), androecious [a 1-aminocyclopropane-1-carboxylate {ACC} synthase 11, acs11], and Monoecious (M ACS2), referred to here as the Female, Androecious, Monoecious (FAM) model, in combination with two genes, gynoecious (g, the WIP family C2H2 zinc finger transcription factor gene WIP1) and the ethylene biosynthetic gene ACC oxidase 2 (ACO2). The F locus, conferring gynoecy and the potential for increasing fruit yield, is defined by a 30.2-kb tandem duplication containing three genes. However, the gene that determines the Female phenotype, and its mechanism, remains unknown. Here, we created a set of mutants and revealed that ACS1G is responsible for gynoecy conferred by the F locus. The duplication resulted in ACS1G acquiring a new promoter and expression pattern in plants carrying the F locus duplication, ACS1G is expressed early in floral bud development, where it functions with ACO2 to generate an ethylene burst. The resulting ethylene represses WIP1 and activates ACS2 to initiate gynoecy. This early ACS1G expression bypasses the need for ACS11 to produce ethylene, thereby establishing a dominant pathway for female floral development. Based on these findings, we propose a model for how these ethylene biosynthesis genes cooperate to control unisexual flower development in cucumber.
Root-secreted bitter triterpene modulates the rhizosphere microbiota to improve plant fitness
Publisher: Springer Science and Business Media LLC
Date: 08-2022
DOI: 10.1038/S41477-022-01201-2
Abstract: Underground microbial ecosystems have profound impacts on plant health
Regulation of plant architecture by a new histone acetyltransferase targeting gene bodies
Publisher: Springer Science and Business Media LLC
Date: 13-07-2020
Harnessing Landrace Diversity Empowers Wheat Breeding for Climate Resilience
Publisher: Cold Spring Harbor Laboratory
Date: 06-10-2023
Publisher Correction: Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors
Publisher: Springer Science and Business Media LLC
Date: 25-09-2023
Vacuolar MATE /DTX protein‐mediated cucurbitacin C transport is co‐regulated with bitterness biosynthesis in cucumber
Publisher: Wiley
Date: 22-02-2023
DOI: 10.1111/NPH.18786
Abstract: Membrane‐localized transporters constitute important components for specialized metabolism in plants. However, due to the vast array of specialized metabolites produced by plants, and the large families of transporter genes, knowledge about the intracellular and intercellular transport of plant metabolites is still in its infancy. Cucurbitacins are bitter and defensive triterpenoids produced mainly in the cucurbits. Using a comparative genomics and multi‐omics approach, a MATE gene ( CsMATE1 ), physically clustered with cucurbitacin C (CuC) biosynthetic genes, was identified and functionally shown to sequester CuC in cucumber leaf mesophyll cells. Notably, the CuC transport process is strictly co‐regulated with CuC biosynthesis. CsMATE1 clustering with bitterness biosynthesis genes may provide benefits and a basis for this feedback regulation on CuC sequestration and biosynthesis. Identification of transport systems for plant‐specialized metabolites can accelerate the metabolic engineering of high‐value‐added compounds by simplifying their purification process.
Biosynthesis, regulation, and domestication of bitterness in cucumber
Publisher: American Association for the Advancement of Science (AAAS)
Date: 28-11-2014
Abstract: Cucurbitacins are triterpenoids that confer a bitter taste in cucurbits such as cucumber, melon, watermelon, squash, and pumpkin. These compounds discourage most pests on the plant and have also been shown to have antitumor properties. With genomics and biochemistry, we identified nine cucumber genes in the pathway for biosynthesis of cucurbitacin C and elucidated four catalytic steps. We discovered transcription factors Bl ( Bitter leaf ) and Bt ( Bitter fruit ) that regulate this pathway in leaves and fruits, respectively. Traces in genomic signatures indicated that selection imposed on Bt during domestication led to derivation of nonbitter cucurbits from their bitter ancestors.
Related Organisations
Chinese Academy Of Agricultural Sciences Institute Of Vegetables And Flowers
Location: China
Agricultural Genomic Institute At Shenzhen, Chinese Academy Of Agricultural Sciences
Location: China
Related Funding Activities
No related grants have been discovered for Sanwen Huang.