ORCID Profile
0000-0002-1805-8599
Current Organisations
Colorado State University
,
University of California Riverside
,
National Health Research Institutes Institute of Biotechnology and Pharmaceutical Research
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Publications
Phytate addition to soil induces changes in the abundance and expression ofBacillusß-propeller phytase genes in the rhizosphere
Publisher: Oxford University Press (OUP)
Date: 19-09-2012
DOI: 10.1111/J.1574-6941.2012.01480.X
Abstract: Phytate-mineralizing rhizobacteria (PMR) perform an essential function for the mineralization of organic phosphorus but little is known about their ecology in soils and rhizosphere. In this study, PCR-based methods were developed for detection and quantification of the Bacillus β-propeller phytase (BPP) gene. Experiments were conducted to monitor the presence and persistence of a phytate-mineralizing strain, Bacillus sp. MQH19, after inoculation of soil microcosms and within the rhizosphere. The occurrence of the BPP gene in natural pasture soils from Chilean Andisols was also examined. The results showed that the Bacillus BPP gene was readily detected in sterile and nonsterile microcosms, and that the quantitative PCR (qPCR) methods could be used to monitor changes in the abundance of the BPP gene over time. Our results also show that the addition of phytate to nonsterile soils induced the expression of the BPP gene in the rhizosphere of ryegrass and the BPP gene was detected in all pasture soils s led. This study shows that phytate addition soils induced changes in the abundance and expression of Bacillus BPP to genes in the rhizosphere and demonstrates that Bacillus BPP gene is cosmopolitan in pasture soils from Chilean Andisols.
Identification of β-propeller phytase-encoding genes in culturable Paenibacillus and Bacillus spp. from the rhizosphere of pasture plants on volcanic soils
Publisher: Oxford University Press (OUP)
Date: 12-11-2011
DOI: 10.1111/J.1574-6941.2010.00995.X
Abstract: Phytate is one of the most abundant sources of organic phosphorus (P) in soils, but must be mineralized by phytase-producing bacteria to release P for plant uptake. Microbial inoculants based on Bacillus spp. have been developed commercially, but few studies have evaluated the ecology of these bacteria in the rhizosphere or the types of enzymes that they produce. Here, we studied the ersity of aerobic endospore-forming bacteria (EFB) with the ability to mineralize phytate in the rhizosphere of pasture plants grown in volcanic soils of southern Chile. PCR methods were used to detect candidate phytase-encoding genes and to identify EFB bacteria that carry these genes. This study revealed that the phytate-degrading EFB populations of pasture plants included species of Paenibacillus and Bacillus, which carried genes encoding β-propeller phytase (BPP). Assays of enzymatic activity confirmed the ability of these rhizosphere isolates to degrade phytate. The phytase-encoding genes described here may prove valuable as molecular markers to evaluate the role of EFB in organic P mobilization in the rhizosphere.
Development of an environmental microarray to study bacterial and archaeal functional genes in Australian soil agroecosystems
Publisher: Elsevier BV
Date: 2012
Metal Chelation in the Rhizosphere
Publisher: American Society of Agronomy, Crop Science Society of America, Soil Science Society of America
Date: 26-10-2015
Bacterial community structure and detection of putative plant growth-promoting rhizobacteria associated with plants grown in Chilean agro-ecosystems and undisturbed ecosystems
Publisher: Springer Science and Business Media LLC
Date: 12-06-2014
Colloid-based multiplexed screening for plant biomass-degrading glycoside hydrolase activities in microbial communities
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C1EE01112J
Rhizosphere interactions between microorganisms and plants govern iron and phosphorus acquisition along the root axis - model and research methods
Publisher: Elsevier BV
Date: 05-2011
Changes in microbial biomass and the metabolic quotient with biochar addition to agricultural soils: A Meta-analysis
Publisher: Elsevier BV
Date: 02-2017
Short-term biochar manipulation of microbial nitrogen transformation in wheat rhizosphere of a metal contaminated Inceptisol from North China plain
Publisher: Elsevier BV
Date: 11-2018
DOI: 10.1016/J.SCITOTENV.2018.06.009
Abstract: While metal immobilization had been increasingly reported with biochar soil amendment (BSA), changes in microbial activity and nitrogen (N) transformation in metal contaminated croplands following biochar addition had been insufficiently addressed. In a field experiment, a Pb/Cd contaminated Inceptisol from North China was amended to topsoil with wheat straw biochar at 0 (CK), 20 (C1) and 40 t ha
Synergistic use of biochar, compost and plant growth‐promoting rhizobacteria for enhancing cucumber growth under water deficit conditions
Publisher: Wiley
Date: 09-06-2017
DOI: 10.1002/JSFA.8393
Abstract: Limited information is available about the effectiveness of biochar with plant growth-promoting rhizobacteria (PGPR) and compost. A greenhouse study was conducted to evaluate the effect of biochar in combination with compost and PGPR (Pseudomonas fluorescens) for alleviating water deficit stress. Both inoculated and un-inoculated cucumber seeds were sown in soil treated with biochar, compost and biochar + compost. Three water levels - field capacity (D0), 75% field capacity (D1) and 50% field capacity (D2) - were maintained. The results showed that water deficit stress significantly suppressed the growth of cucumber however, synergistic use of biochar, compost and PGPR mitigated the negative impact of stress. At D2, the synergistic use of biochar, compost and PGPR caused significant increases in shoot length, shoot biomass, root length and root biomass, which were respectively 88, 77, 89 and 74% more than in the un-inoculated control. Significant improvements in chlorophyll and relative water contents as well as reduction in leaf electrolyte leakage demonstrated the effectiveness of this approach. Moreover, the highest population of P. fluorescens was observed where biochar and compost were applied together. These results suggest that application of biochar with PGPR and/or compost could be an effective strategy for enhancing plant growth under stress. © 2017 Society of Chemical Industry.
Related Organisations
Institute Of Chemistry Academia Sinica
Location: Taiwan, Province of China
University Of California Riverside
Location: United States of America
National Health Research Institutes Institute Of Biotechnology And Pharmaceutical Research
Location: Taiwan, Province of China
National Tsing-Hua University Department Of Chemistry
Location: Taiwan, Province of China
Sun Yat-Sen University Department Of Chemistry
Location: Taiwan, Province of China
Related Funding Activities
No related grants have been discovered for David Crowley.