ORCID Profile
0000-0001-7423-9696
Current Organisation
University of Southampton
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Publisher: Wiley
Date: 07-01-2005
Publisher: Wiley
Date: 14-08-2009
DOI: 10.1113/EXPPHYSIOL.2009.047340
Abstract: The nutritional environment during development and even prior to conception may contribute to cardiovascular risk. In mature adult female sheep, we investigated the effect of preconceptional and periconceptional maternal nutritional restriction on the vascular reactivity of arteries from four vascular beds supplying the heart, thorax, kidney and hindlimb. Welsh Mountain ewes received 100% of nutrient requirements throughout gestation (control group, C, n = 18), or 50% of nutrient requirements for 30 days prior to conception (preconceptional group, PRE, n = 20) or for 15 days either side of conception (periconceptional group, PERI, n = 31) and 100% thereafter. In 3.5‐year‐old female offspring, the left anterior descending coronary (LAD), left internal thoracic (LITA), right renal and second and third order femoral arteries were dissected and their reactivity was assessed by organ bath or wire myography. Vasoconstrictor responses were greater in both LAD and LITA from PERI offspring compared with C ( P 0.01), while vasoconstriction was unaffected by maternal diet in arteries from the renal and femoral circulations ( P = n.s.). Endothelium‐dependent and ‐independent vasodilatation was attenuated in third order femoral arteries of PRE and PERI groups compared with C ( P 0.05). Endothelium‐independent vasodilatation was attenuated in both the LAD and renal arteries in the PERI group compared with C ( P 0.05). These data show that moderate maternal undernutrition either prior to or around conception affects vascular function of adult offspring. The effect depends on the timing of the insult, but also on the vascular bed studied and vessel hierarchy in the vascular tree.
Publisher: Public Library of Science (PLoS)
Date: 06-03-2014
Publisher: Wiley
Date: 07-12-2007
DOI: 10.1111/J.1471-0528.2007.01607.X
Abstract: To determine the role of carotid sinus innervation on differential fetal organ growth during maternal nutrient restriction in late pregnancy. Randomised controlled study. University research facility. Thirty-nine Merino ewes. At 113 days gestational age (dGA), fetuses were bilaterally carotid sinus denervated or sham denervated. From 118 dGA, the surgery groups were sub ided into two dietary groups, and their ewes were fed 100% of nutrient requirements or 50% until tissue collection at 140 dGA. This provided four groups (sham/control diet, sham/restricted diet, denervated/control diet and denervated/restricted diet). Fetal organ weights and hormone levels and maternal weight change during the dietary restriction. Adrenal glands were larger in sham/restricted diet fetuses than in sham/control diet or denervated/restricted diet fetuses (P < 0.05). Fetal adrenal weight and brain-to-liver weight ratio were positively related to maternal weight change during the nutritional challenge in sham fetuses only (P < 0.05). Fetal liver weight was negatively related to maternal weight change during nutritional challenge in sham fetuses only (P < 0.05). We have shown a reduction in liver growth but sparing of adrenal growth in response to moderate maternal undernutrition, which is dependent on intact carotid body innervation. This suggests a new role for the carotid bodies in the control of differential organ growth during such undernutrition.
Publisher: Wiley
Date: 14-06-2010
Publisher: Elsevier BV
Date: 02-2002
Abstract: The purpose of this study was to determine how chronic hypoxia and/or protein malnutrition in ovo affect growth in developing chicks. Chicken eggs were incubated under normoxic (21% oxygen n = 30 eggs) or hypoxic (14% oxygen n = 80 eggs) conditions. Hypoxia was imposed from day 0 (n = 38 eggs), day 10 (n = 22 eggs), or from day 0 to 10 (n = 20 eggs). Protein malnutrition alone (n = 20 eggs) or in combination with hypoxia (n = 24 eggs) was induced by removal of 10% of the estimated total albumin content of the egg. Embryos/chicks were killed and weighed at day 10, 15, or immediately after hatch organs were removed and weighed. Embryos to which hypoxia was imposed from day 0 weighed less than control embryos at day 10, which stayed the same until hatch (64.67% +/- 3.56% egg mass vs 69.36% +/- 3.90% [mean +/- SD] P <.05). Malnourished chicks at day 15 and at hatch (63.42% +/- 4.28% P <.05) weighed less than control chicks, as did malnourished plus hypoxia chicks (59.74% +/- 3.41% P <.001). Malnourished plus hypoxia chicks weighed less than malnourished chicks alone (P <.05). Embryos that were hypoxic from day 0 to 10 weighed less than control embryos at day 15 (P <.05), but not at hatch. At hatch, neither hypoxia nor malnutrition decreased crown-rump length. Brain and heart weights were increased in both malnourished groups, but not chicks that were hypoxic from day 0. Chick embryos exposed to malnutrition show asymmetric growth restriction with relative sparing of the brain and heart. Early growth restriction that was induced by hypoxia from the beginning of incubation is reversed by the restoration of normoxia at mid incubation.
Publisher: Cambridge University Press (CUP)
Date: 22-03-2017
DOI: 10.1017/S2040174417000149
Abstract: Placental transport of vitamin D and other nutrients (e.g. amino acids, fats and glucose) to the fetus is sensitive to maternal and fetal nutritional cues. We studied the effect of maternal calorific restriction on fetal vitamin D status and the placental expression of genes for nutrient transport [aromatic T-type amino acid transporter-1 (TAT-1) triglyceride hydrolase/lipoprotein uptake facilitator lipoprotein lipase (LPL)] and vitamin D homeostasis [CYP27B1 vitamin D receptor (VDR)], and their association with markers of fetal cardiovascular function and skeletal muscle growth. Pregnant sheep received 100% total metabolizable energy (ME) requirements (control), 40% total ME requirements peri-implantation [PI40, 1–31 days of gestation (dGA)] or 50% total ME requirements in late gestation (L, 104–127 dGA). Fetal, but not maternal, plasma 25-hydroxy-vitamin D (25OHD) concentration was lower in PI40 and L maternal undernutrition groups ( P .01) compared with the control group at 0.86 gestation. PI40 group placental CYP27B1 messenger RNA (mRNA) levels were increased ( P .05) compared with the control group. Across all groups, higher fetal plasma 25OHD concentration was associated with higher skeletal muscle myofibre and capillary density ( P .05). In the placenta, higher VDR mRNA levels were associated with higher TAT-1 ( P .05) and LPL ( P .01) mRNA levels. In the PI40 maternal undernutrition group only, reduced fetal plasma 25OHD concentration may be mediated in part by altered placental CYP27B1. The association between placental mRNA levels of VDR and nutrient transport genes suggests a way in which the placenta may integrate nutritional cues in the face of maternal dietary challenges and alter fetal physiology.
Publisher: American Physiological Society
Date: 2007
DOI: 10.1152/AJPENDO.00253.2006
Abstract: The early-life environment has implications for risk of adult-onset diseases, such as glucose intolerance, insulin insensitivity, and obesity, effects that may occur with or without reduced birth weight. We determined the consequences of nutrient restriction in early gestation and early postnatal life and their interactions on postnatal growth, body composition, and glucose handling. Ewes received 100% (C, n = 39) or 50% nutritional requirements (U, n = 41) from 1 to 31 days gestation and 100% thereafter. Male and female offspring (singleton/twin) from C and U ewes were then fed either ad libitum (CC n = 22, UC n = 19) or to reduce body weight to 85% of target from 12 to 25 wk of age (CU n = 17, UU n = 22) and ad libitum thereafter. At 1.5 and 2.5 yr, glucose handling was determined by area under the curve (AUC) for glucose and insulin concentrations following intravenous glucose (0.5 g/kg body wt). Insulin sensitivity was determined at 2.5 yr following intravenous insulin (0.5 IU/kg). In females, postnatal undernutrition reduced ( P 0.05) glucose AUC at both ages, regardless of prenatal nutrition. Postnatal undernutrition did not affect insulin secretion in females but enhanced insulin-induced glucose disappearance in singletons. Poor early postnatal growth was associated with increased fat in females. In males, glucose tolerance was unaffected by undernutrition despite changes in insulin AUC dependent on age, treatment, and single/twin birth. Nutrition in early postnatal life has long-lasting, sex-specific effects on glucose handling in sheep, likely due, in females, to enhanced insulin sensitivity. Improved glucose utilization may aid weight recovery but have negative implications for glucose homeostasis and body composition over the longer term.
Publisher: Proceedings of the National Academy of Sciences
Date: 29-05-2007
Abstract: The early life environment has long-term implications for the risk of developing cardiovascular (CV) disease in adulthood. Fetal responses to changes in maternal nutrition may be of immediate benefit to the fetus, but the long-term effects of these adaptations may prove detrimental if nutrition in postnatal life does not match that predicted by the fetus on the basis of its prenatal environment. We tested this predictive adaptive response hypothesis with respect to CV function in sheep. We observed that a mismatch between pre- and postnatal nutrient environments induced an altered CV function in adult male sheep that was not seen when environments were similar. Sheep that received postnatal undernutrition alone had altered growth, CV function, and basal hypothalamo–pituitary–adrenal axis activity in adulthood. Prenatal undernutrition induced greater weight gain by weaning compared with the prenatal control diet, which may provide a reserve in the face of a predicted poor diet in later life. In an adequate postnatal nutrient environment (i.e., relatively mismatched), these offspring exhibited cardiac hypertrophy and altered CV function in adulthood. These data support the concept that adult CV function can be determined by developmental responses to intrauterine nutrition made in expectation of the postnatal nutritional environment, and that if these predictions are not met, the adult may be maladapted and at greater risk of CV disease. Our findings have substantial implications for devising strategies to reduce the impact of a mismatch in nutrition levels in humans undergoing rapid socio-economic transitions in both developing and developed societies.
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Lucy Green.