ORCID Profile
0000-0002-6079-2105
Current Organisation
University Of Strathclyde
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Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5FD90023A
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6FD90013E
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6FD90011A
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6FD90014C
Publisher: Springer Science and Business Media LLC
Date: 04-06-2019
DOI: 10.1038/S41598-019-44569-6
Abstract: Gold nanoparticles (AuNPs) have been extensively used as nanomaterials for theranostic applications due to their multifunctional characteristics in therapeutics, imaging, and surface modification. In this study, the unique functionalities of exosome-derived membranes were combined with synthetic AuNPs for targeted delivery to brain cells. Here, we report the surface modification of AuNPs with brain-targeted exosomes derived from genetically engineered mammalian cells by using the mechanical method or extrusion to create these novel nanomaterials. The unique targeting properties of the AuNPs after fabrication with the brain-targeted exosomes was demonstrated by their binding to brain cells under laminar flow conditions as well as their enhanced transport across the blood brain barrier. In a further demonstration of their ability to target brain cells, in vivo bioluminescence imaging revealed that targeted-exosome coated AuNPs accumulated in the mouse brain after intravenous injection. The surface modification of synthetic AuNPs with the brain-targeted exosome demonstrated in this work represents a highly novel and effective strategy to provide efficient brain targeting and shows promise for the future in using modified AuNPs to penetrate the brain.
Publisher: Elsevier BV
Date: 2012
DOI: 10.1016/J.TALANTA.2011.11.051
Abstract: A simple controlled chemical reduction of 1,3,5-trinitro-1,3,5-triazacyclohexane (RDX) and related nitramine compounds with zinc amalgam generates species that elicit intense chemiluminescence with tris(2,2'-bipyridine)ruthenium(III), which extends this widely utilised chemiluminescence reagent to a new class of analyte and presents a sound chemical basis for a screening test for nitramine high explosives. Examination of the chemiluminescence profiles under stopped-flow conditions revealed contributions from multiple transient species formed in the initial reduction step.
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2AN00703G
Abstract: SERS for antibiotic resistance diagnosis.
Publisher: Wiley
Date: 07-10-2010
DOI: 10.1002/JRS.2495
Publisher: Wiley
Date: 09-2016
Abstract: Drugs targeting MDM2's hydrophobic pocket activate p53. However, these agents act allosterically and have agonist effects on MDM2's protein interaction landscape. Dominant p53-independent MDM2-drug responsive-binding proteins have not been stratified. We used as a variable the differential expression of MDM2 protein as a function of cell density to identify Nutlin-3 responsive MDM2-binding proteins that are perturbed independent of cell density using SWATH-MS. Dihydrolipoamide dehydrogenase, the E3 subunit of the mitochondrial pyruvate dehydrogenase complex, was one of two Nutlin-3 perturbed proteins identified fours hour posttreatment at two cell densities. Immunoblotting confirmed that dihydrolipoamide dehydrogenase was induced by Nutlin-3. Depletion of MDM2 using siRNA also elevated dihydrolipoamide dehydrogenase in Nutlin-3 treated cells. Mitotracker confirmed that Nutlin-3 inhibits mitochondrial activity. Enrichment of mitochondria using TOM22+ immunobeads and TMT labeling defined key changes in the mitochondrial proteome after Nutlin-3 treatment. Proximity ligation identified rearrangements of cellular protein-protein complexes in situ. In response to Nutlin-3, a reduction of dihydrolipoamide dehydrogenase/dihydrolipoamide acetyltransferase protein complexes highlighted a disruption of the pyruvate dehydrogenase complex. This coincides with an increase in MDM2/dihydrolipoamide dehydrogenase complexes in the nucleus that was further enhanced by the nuclear export inhibitor Leptomycin B. The data suggest one therapeutic impact of MDM2 drugs might be on the early perturbation of specific protein-protein interactions within the mitochondria. This methodology forms a blueprint for biomarker discovery that can identify rearrangements of MDM2 protein-protein complexes in drug-treated cells.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5FD90022K
Publisher: American Chemical Society (ACS)
Date: 06-01-2016
DOI: 10.1021/ACS.ANALCHEM.5B02776
Abstract: A significant advantage of using surface enhanced Raman scattering (SERS) for DNA detection is the capability to detect multiple analytes simultaneously within the one s le. However, as the analytes approach the metallic surface required for SERS, they become more concentrated and previous studies have suggested that different dye labels will have different affinities for the metal surface. Here, the interaction of single stranded DNA labeled with either fluorescein (FAM) or tetramethylrhodamine (TAMRA) with a metal surface, using spermine induced aggregated silver nanoparticles as the SERS substrate, is investigated by analyzing the labels separately and in mixtures. Comparison studies were also undertaken using the dyes in their free isothiocyanate forms, fluorescein isothiocyanate (F-ITC) and tetramethylrhodamine isothiocyanate (TR-ITC). When the two dyes are premixed prior to the addition of nanoparticles, TAMRA exerts a strong masking effect over FAM due to a stronger affinity for the metal surface. When parameters such as order of analyte addition, analysis time, and analyte concentration are investigated, the masking effect of TAMRA is still observed but the extent changes depending on the experimental parameters. By using bootstrap estimation of changes in SERS peak intensity, a greater insight has been achieved into the surface affinity of the two dyes as well as how they interact with each other. It has been shown that the order of addition of the analytes is important and that specific dye related interactions occur, which could greatly affect the observed SERS spectra. SERS has been used successfully for the simultaneous detection of several analytes however, this work has highlighted the significant factors that must be taken into consideration when planning a multiple analyte assay.
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1AN00726B
Abstract: Duplex SERS-based lateral flow testing for C. diff bacterial infection using new biomarker, SlpA, and ToxB within 20 minutes.
Publisher: American Chemical Society (ACS)
Date: 12-03-2010
DOI: 10.1021/JA908117A
Publisher: American Chemical Society (ACS)
Date: 05-03-2012
DOI: 10.1021/IC202197G
Abstract: Gold nanoparticles (AuNPs) can be used as delivery vehicles for platinum anticancer drugs, improving their targeting and uptake into cells. Here, we examine the appropriateness of different-sized AuNPs as components of platinum-based drug-delivery systems, investigating their controlled synthesis, reproducibility, consistency of drug loading, and stability. The active component of cisplatin was tethered to 25, 55, and 90 nm AuNPs, with the nanoparticles being almost spherical in nature and demonstrating good batch-to-batch reproducibility (24.37 ± 0.62, 55.2 ± 1.75, and 89.1 ± 2.32 nm). The size distribution of 25 nm AuNPs has been significantly improved, compared with a previous method that produces polydispersed nanoparticles. Attachment of platinum to the AuNP surface through a poly(ethylene glycol) (PEG) linker exhibits an increase in the drug loading with increasing particle size: 25 nm (815 ± 106 drug molecules per AuNP), 55 nm (14216 ± 880), and 90 nm (54487 ± 15996). The stability of the naked, PEGylated, and platinum-conjugated nanoparticles has been examined over time under various conditions. When stored at 4 °C, there is minimal variation in the diameter for all three AuNP sizes variation after 28 days for the 25 nm AuNPs was 2.4% 55 nm, 3.3% and 90 nm, 3.6%. The 25 nm AuNPs also demonstrate minimal changes in UV-visible absorbance over the same time period.
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Duncan Graham.