Frank Dunshea

Age, sex, and weight at weaning influence organ weight and gastrointestinal development of weanling pigs

Publisher: CSIRO Publishing

Date: 2003

DOI: 10.1071/AR02156

Abstract: The present study was designed to determine the interrelationships between sex, weaning age, and weaning weight on aspects of physiological and gastrointestinal development in pigs. Forty-eight Large White × Landrace pigs were used in a factorial arrangement with the respective factors being: age at weaning (14 or 28 days), weight at weaning (heavy or light), sex (boar or gilt), and time after weaning (1, 7, and 14 days). At weaning, 48 pigs were removed from the sow: 16 pigs were then fasted for 24 h before euthanasia for determination of organ weights, gut histology, and enzymology, and 32 pigs were offered a high quality pelleted weaner diet ad libitum for subsequent assessment of organ weights, histology, and enzymology at 7 and 14 d after weaning. On Day 6 and 13 after weaning, 2 pigs from each group had their feed removed, and 24 h later were euthanased and similar measurements were taken. In general, the data highlighted the overall gastrointestinal underdevelopment of pigs weaned at 2 weeks of age and of pigs weaned light-for-age at either 2 or 4 weeks. Heavier body organs, gastrointestinal organs, and accessory digestive organs observed after weaning, except for the spleen, presumably reflected the increase in substrates available for cellular growth as feed intake increased after weaning, and the development of organs required to process this feed. Interestingly, the relative weights (% of liveweight) of the stomach and small intestine and, to a lesser extent, the caecum and colon, were greater in the light, 14-day-old weaned pigs, but these differences diminished with increasing time after weaning. Consistent effects due to age, weight, and sex were not observed for villous height and crypt depth, or for the specific activities of the brush-border and pancreatic enzymes measured. However, increases (P 0.001) in the activities of maltase (P� �0.001), glucoamylase (P 0.001), and sucrase (P = 0.020) (all expressed per gram of mucosa), and that of trypsin (per gram of pancreas), occurred by 14 days after weaning. This most likely reflected the inducible nature of these enzymes in response to the increasing intake of substrates provided in the diet. In contrast, the specific activity of lactase declined (P = 0.012) in the first 14 days after weaning. These data suggest that pigs weaned at 2 weeks of age and pigs weaned light-for-age at either 2 or 4 weeks have a less developed gastrointestinal tract, and that its development after weaning might proceed differently to that of pigs weaned older and heavier.

A GnRF vaccine (Improvac®) and porcine somatotropin (Reporcin®) have synergistic effects upon growth performance in both boars and gilts

Publisher: CSIRO Publishing

Date: 2003

DOI: 10.1071/AR02037

Abstract: The use of a vaccine (Improvac) directed against gonadotrophin-releasing factor (GnRF), to prevent boar taint in male pigs, is also associated with an increase in feed consumption, growth, and higher deposition of subcutaneous fat. Daily administration of porcine somatotropin (pST) consistently increases average daily gain, and feed conversion efficiency, and reduces subcutaneous fat, particularly in gilts. The objective of this study was to examine the effect of combining these treatments in boars and gilts. Forty-eight pigs (Large White × Landrace), housed in idually, were used in a 2 × 2 × 2 factorial experiment with the respective factors being vaccination with GnRF vaccine (at 14 and 18 weeks of age), daily injection with pST (from 18 weeks to slaughter at 22 weeks), and sex (boars and gilts). Performance was measured over the period of pST administration. Over the entire 28 days of the study, daily gain was lower for gilts than for boars (1216 v. 1410 g/day, P 0.001), was increased by pST (1219 v. 1407 g/day, P 0.001), but not significantly altered by vaccination against GnRF (1284 v. 1342 g/day, P = 0.15). However, GnRF vaccination increased daily gain in the second 2 weeks of the study, and overall, there were interactions such that the vaccine increased gain in all groups except boars not receiving pST. Feed intake over the study period was less for gilts than for boars (3139 v. 3407 g/day, P 0.001), was decreased by pST (3537 v. 3009 g/day, P 0.001), and increased by vaccination against GnRF (3139 v. 3407 g/day, P 0.001). When averaged over the entire study, feed conversion ratio (FCR) was higher for gilts than for boars (2.76 v. 2.51, P = 0.013), was decreased by pST (3.05 v. 2.22, P 0.001), but unchanged by vaccination against GnRF (2.62 v. 2.65, P = 0.70). Fat deposition was lower in gilts than in boars (335 v. 378 g/day, P = 0.037), was decreased by pST treatment ((442 v. 271 g/day, P 0.001), and increased by vaccination against GnRF (333 v. 379 g/day, P = 0.026). In boars, fat androstenone was reduced by vaccination against GnRF (0.10 v. 2.47 μg/g, P 0.001) and tended to be reduced by pST (1.71 v. 2.44, P = 0.10), but only vaccination against GnRF reduced skatole (0.174 v. 0.050 μg/g, P 0.001). The combination of the treatments in all cases was at least additive, and in some cases synergistic. The concomitant vaccination against GnRF and treatment with pST affords an opportunity to increase growth rate and feed intake above control levels and above those of pigs treated with either treatment alone.

IGF-I is not a useful marker for nutritional status in the growing pig under commercial conditions

Publisher: CSIRO Publishing

Date: 2001

DOI: 10.1071/AR00096

Abstract: Plasma hormone concentrations were measured in gilts after fasting, long-term protein restriction, or supplementation. In 11-week-old pigs fasted overnight, plasma insulin, glucagon, gastrin, urea, and glucose were increased 30 min after re-feeding (P 0.05), whereas IGF-I did not change. In 16-week-old gilts fed a standard commercial diet [14.6% crude protein (CP)], or a high-protein diet (16.7% CP) for 4 weeks, the high-protein diet increased weight gain (13% P 0.05) and carcass weight (4% P 0.05), but did not alter plasma IGF-I, insulin, or glucagon. In 10-week-old gilts fed high-protein diets (19.4% and 18.3% CP), or low-protein diets (15.5% and 13.3% CP) for 12 weeks during the grower and finisher phases, respectively, the low-protein diet decreased weight gain (18% P 0.001) and carcass weight (11% P 0.01), with a marked increase in plasma glucagon (P 0.05), no change in insulin, and only a trend towards decreased IGF-I (P = 0.1). The pigs were more sensitive to altered dietary protein at 10 weeks of age than at 16 weeks. Plasma IGF-I was not responsive to the short-term effects of feeding or the long-term effects of dietary protein. Glucagon could provide a useful marker for nutritional status in young pigs, provided that time of feeding is taken into account.

Effects of Context and Virtual Reality Environments on the Wine Tasting Experience, Acceptability, and Emotional Responses of Consumers

Publisher: MDPI AG

Date: 14-02-2020

DOI: 10.3390/FOODS9020191

Abstract: Wine tasting is a multidimensional experience that includes contextual information from tasting environments. Formal sensory tastings are limited by the use of booths that lack ecological validity and engagement. Virtual reality (VR) can overcome this limitation by simulating different environmental contexts. Perception, sensory acceptability, and emotional responses of a Cabernet Sauvignon wine under traditional sensory booths, contextual environments, and VR simulations were evaluated and compared. Participants (N = 53) performed evaluations under five conditions: (1) traditional booths, (2) bright-restaurant (real environment with bright lights), (3) dark-restaurant (real environment with dimly lit candles), (4) bright-VR (VR restaurant with bright lights), and (5) dark-VR (VR restaurant with dimly lit candles). Participants rated the acceptability of aroma, sweetness, acidity, astringency, mouthfeel, aftertaste, and overall liking (9-point hedonic scale), and intensities of sweetness, acidity, and astringency (15-point unstructured line-scale). Results showed that context (booths, real, or VR) affected the perception of the wine’s floral aroma (dark-VR = 8.6 vs. booths = 7.5). Liking of the sensory attributes did not change under different environmental conditions. Emotional responses under bright-VR were associated with “free”, “glad”, and “enthusiastic” however, under traditional booths, they were related to “polite” and “secure”. “Nostalgic” and “daring” were associated with dark-VR. VR can be used to understand contextual effects on consumer perceptions.

Nutritional manipulation increases intramuscular fat levels in the Longissimus muscle of female finisher pigs

Publisher: CSIRO Publishing

Date: 2003

DOI: 10.1071/AR03009

Abstract: Fifty crossbred (Large White × Landrace × Duroc) female finisher pigs were used to determine the effect of nutritional manipulations on intramuscular fat deposition. The main nutritional treatments were (i) Control (pigs fed a commercial grower diet), (ii) –15% P : DE (pigs fed a 15% reduced protein : digestible energy diet during the grower phase), (iii) –30% P : DE (pigs fed a 30% reduced P : DE diet during the grower phase), (iv) +6% Fat (pigs fed diet supplemented with 6% additional fat during the grower phase), and (v) –Vit A (pigs fed a control diet with no supplemented Vit A in the mineral vitamin premix during the grower and finisher phase). Pigs fed the 30% reduced P : DE diet had an inferior feed to liveweight gain (feed conversion ratio, FCR) for the combined grower and finisher growth period (P = 0.036) compared with the other dietary treatments. There were no significant differences in average daily gain (ADG) or voluntary feed intake (VFI) for the grower, finisher, and the combined grower and finisher phases, or liveweight at slaughter between the dietary treatments. Pigs fed the 15% (1.9%) and 30% (2.7%) reduced P : DE diet, and the Vit A-restricted diet (2.0%), had significantly higher intramuscular fat (IMF) levels compared with pigs fed the other diets. Pigs fed the control diet had higher (P = 0.002) carcass dressing percentage compared with pigs fed the other diets. There were no significant dietary effects on total fat tissue (P�=�0.661), total lean muscle tissue (P = 0.637), and total bone mineral (P = 0.367) content. In conclusion, these data indicate that feeding pigs grower diets with 15% and 30% reduced P : DE, and grower and finisher diets restricted in Vit A, improved the IMF levels in the Longissimus muscle. The increase in IMF levels in pigs fed the 15% reduced P : DE diet, and grower and finisher diets restricted in Vit A, improved the IMF levels without having a detrimental effect on growth performance and carcass quality.

Dietary nano-chromium tripicolinate increases feed intake and decreases plasma cortisol in finisher gilts during summer

Publisher: Springer Science and Business Media LLC

Date: 30-09-2014

DOI: 10.1007/S11250-014-0673-7

Abstract: Chromium (Cr) is an essential mineral element and has been used in pig diets to improve growth performance, insulin sensitivity, immune response and carcase traits and to reduce heat or other stress responses. The aims of thiss study were to determine the impact of nano-sized chromium tripicolinate (nCrPic) on growth performance, feed efficiency and carcase characteristics of finisher gilts during the summer period. A total of 60 finisher Large White x Landrace gilts were stratified on initial weight and then within strata randomly allocated into two treatment groups in three replicates during mid-summer for 28 days. All pigs were housed in in idual pens and had ad libitum access to feed and water. Pigs were fed either a control finisher diet (wheat-based diet containing 13.8 MJ digestible energy (DE) per kilogram and 0.56 g available lysine/MJ DE) or a control diet containing 400 ppb Cr as nCrPic. Dietary nCrPic supplementation increased feed intake by 6 % over the entire study (P = 0.05). In particular, dietary nCrPic increased average daily feed intake (ADFI) by 8 % (P = 0.02) during the final 2 weeks of the study. Moreover, dietary nCrPic tended to improve average daily feed (ADFI) over the entire study (P = 0.09). However, there were no significant effects of nCrPic on feed conversion ratio (FCR), final weight, hot standard carcase weight (HCWT), P2 depth or dressing percentage. Plasma cortisol was decreased by 25 % (P = 0.06) by dietary nCrPic supplementation. However, there were no effects of nCrPic on plasma glucose, insulin and nonesterified fatty acids (NEFA), might because of the higher feed intake. In conclusion, this study demonstrates that dietary nCrPic supplementation at 400 ppb can increase feed intake in finisher gilts during mid-summer, suggesting that nCrPic can ameliorate some of the negative effects of heat stress in pigs, possibly via decreased of circulating cortisol.

Relationships between in vivo and in vitro lipid metabolism in lactating goats

Publisher: CSIRO Publishing

Date: 2000

DOI: 10.1071/AR99064

Abstract: Multiparous Sannen dairy goats were used to compare in vivo and in vitro lipid metabolism. Goats were infused simultaneously with a mixture of [2-3H]-glycerol and of [1-14C]-palmitic, -stearic, and -oleic acid complexed in plasma in early (Day 11, n = 4), mid (Day 37, n = 4), and late (Day 80, n = 3) lactation to determine in vivo lipid kinetics. Perirenal adipose tissue biopsies were obtained and used to measure in vitro lipid kinetics under both basal and norepinephrine-stimulated conditions. Plasma non-esterified fatty acid (NEFA) concentrations and NEFA entry rate were higher (P 0.05) during early compared with mid and late lactation. Plasma glucose and glycerol concentrations and glycerol entry rate were not different between stages of lactation. Although variable, in vitro lipogenesis increased more than 10-fold (P = 0.077) between early and late lactation, whereas there were no significant differences between NEFA and glycerol release at any stage of lactation. In vitro lipid metabolism, when expressed per gram of adipose tissue, was poorly correlated with in vivo lipid. However, when in vitro NEFA release was extrapolated to the whole animal the correlations were markedly improved. These data demonstrate that the reduction in lipid reserves which occurs during early lactation is the result of increased NEFA mobilisation and reduced lipogenesis rather than increased lipolysis. Although more variable than in vivo measures of lipid metabolism, in vitro metabolism of adipose tissue can reflect metabolic status.

Feeding slowly fermentable grains has the potential to ameliorate heat stress in grain-fed wethers1,2

Publisher: Oxford University Press (OUP)

Date: 07-2016

DOI: 10.2527/JAS.2016-0295

Abstract: During heat stress (HS), livestock reduce metabolic heat production by lowering activity and feed intake. Because this has obvious consequences for productivity, the aim of these experiments was to investigate nutritional methods for reducing digestive metabolic heat production, thereby allowing livestock more opportunity to dissipate excess heat. In the first experiment, the fermentation rates of corn and wheat grains were compared in an in vitro gas production system containing buffered rumen fluid. This experiment showed that corn had a slower (-15% < 0.001) rate of gas production than wheat and no differences in total amount of gas production after 24 h of incubation. In the second experiment, we hypothesized that the lower rate of fermentation of corn would reduce metabolic heat load in wethers and, in turn, improve tolerance to HS. Twenty-two Merino × Poll Dorset wethers were housed in 2 climate-controlled rooms and were fed either corn grain plus forage (CD 39% starch) or wheat grain plus forage (WD 37% starch) during 3 experimental periods: period 1 (P1), which consisted of 7 d of thermoneutral conditions (18 to 21°C and 40 to 50% relative humidity [RH]) and restricted feed intake (1.3 times maintenance) period 2 (P2), which consisted of 7 d of HS (28 to 38°C and 30 to 50% RH) and restricted feed intake and period 3 (P3), which consisted of 7 d of HS as in P2 with unrestricted feed intake (1.5 times maintenance) in a randomized control experiment. Water was offered ad libitum. The level of HS was quantified by increases in rectal temperature (RT), respiration rate (RR), and left and right flank skin temperature (LFT and RFT, respectively) and blood acid-base balance. Rectal temperature, RR, LFT, and RFT were elevated ( < 0.001) during HS, especially when wethers had unrestricted feed intake (P3). Wethers fed CD had lower RR, RT, LFT, and RFT ( < 0.001) than wethers fed WD, and this benefit was greatest during HS (P2 and P3). The reduction in RR with CD resulted in less CO exhalation (greater partial pressure of CO2) and greater HCO3 ( < 0.05) than with WD, indicating reduced efforts to dissipate heat by evaporative heat loss via panting. The greatest heat from fermentation was apparent in WD wethers, which had elevated LFT compared with RFT ( < 0.001). Crucially, this large difference was not observed with the CD wethers, indicating that the slow rate of fermentation of CD was expressed as low heat released during feed fermentation in the rumen. These data demonstrated that feeding CD may be a useful management strategy to reduce the impact of high environmental heat loads in sheep.

Antioxidant effects of rosemary extract and whey powder on te oxidative stability of wiener sausages during 10 months frozen storage.

Publisher: Elsevier BV

Date: 10-2002

DOI: 10.1016/S0309-1740(01)00249-2

Abstract: Twelve (Large White×Landrace) gilts were randomly allotted in a 2×2 factorial design with the respective factors being dietary vitamin E (10 or 200 mg/kg feed) and dietary fishmeal (0 or 5%). Wiener sausages were manufactured with or without antioxidants such as rosemary extract and sweet whey powder from meat obtained from the animals after slaughter and stored for 5 days at 4 °C. The oxidative stability of the wieners was examined over ten months of frozen storage. Lipid oxidation in the product was measured by means of thiobarbituric acid reactive substances (TBARS) and fluorescence shift. Sensory evaluation of the product to detect oxidative changes was also carried out. The fluorescence shift method was unsatisfactory in the case of wieners containing rosemary extract, as it appeared that the extract contained compounds that fluoresced and therefore interfered with the method. No lipid oxidation as measured by TBARS, fluorescence shift and sensory analysis was observed in wieners stored at -20 °C for 10 months. The oxidative stability of wieners was unaffected (P>0.05) by dietary treatments or by the addition of antioxidants. The high oxidative stability of the wieners, even in the absence of antioxidants, could be due to sodium erythorbate present in the formulation as an additional antioxidant.

Effect of dietary vitamin E, fishmeal and wood and liquid smoke on the oxidative stability of bacon during 16 weeks' frozen storage

Publisher: Elsevier BV

Date: 09-2002

DOI: 10.1016/S0309-1740(01)00226-1

Abstract: Twelve (Large White×Landrace) gilts were randomly allotted in a 2×2 factorial design with the respective factor being dietary vitamin E (10 or 200 mg/kg feed) and dietary fishmeal (0 or 5%). Bacon was manufactured from the meat obtained from the animals after slaughter using wood smoke only or a combination of liquid and wood smoke. The oxidative stability of the bacon was examined over 16 weeks of frozen storage. Lipid oxidation in the product was measured by means of thiobarbituric acid reactive substances (TBARS) and fluorescence shift. Dietary fishmeal supplementation increased lipid oxidation in bacon, while dietary vitamin E supplementation reduced lipid oxidation in the product. Lipid oxidation in frozen bacon was successfully reduced when bacon was manufactured from pigs fed a diet supplemented with or without 200 mg of α-tocopherol per kilogram of feed and processed with a combination of liquid and wood smoke. It is concluded that bacon processed with a combination of liquid and wood smoke was significantly less (P<0.001) susceptible to lipid oxidation than bacon processed with wood smoke only.

Porcine somatotropin alters insulin response in growing pigs by reducing insulin sensitivity rather than changing responsiveness

Publisher: Elsevier BV

Date: 07-2012

DOI: 10.1016/J.DOMANIEND.2012.02.001

Abstract: Exogenous porcine somatotropin (pST) treatment consistently improves growth performance and reduces fat deposition in pigs, and it is hypothesized that one component of the mechanism is through altering the sensitivity and/or responsiveness to insulin. Therefore, a study was conducted to investigate the effect of pST treatment on whole-body glucose metabolism in response to varying doses of insulin. Eight barrows were surgically prepared with indwelling catheters and randomly assigned to one of two treatment groups (0 or 120 μg pST/kg BW · d) for 13 d. Whole-body glucose kinetics were measured during infusion of [6-(3)H]-glucose under basal conditions and during hyperinsulinemic-euglycemic cl s at various insulin infusion rates (7, 28, and 140, and 14, 70, and 280 ng insulin/kg BW · min) and alterations in the dose-response parameters were calculated with nonlinear regression. Treatment with pST increased basal plasma concentrations of glucose (36% P = 0.005), insulin (276% P = 0.001), and NEFAs (177% P = 0.01) and decreased the rate of glucose disappearance (-59% P = 0.001). The responsiveness (maximum response) for steady state glucose infusion rate to maintain glycemia was not altered by pST (112 vs 106 μmol/min · kg P = 0.78), whereas the sensitivity (effective dose at 50% of maximum response) was increased almost 7-fold (1.3 vs 8.7 ng/mL P = 0.027). Similar responses were observed for rate of glucose disappearance and insulin-dependent glucose utilization. Therefore, pST-induced insulin resistance with regard to whole-body glucose uptake is due to a reduced sensitivity to insulin, rather than a change in responsiveness.

Evaluation of the n-alkane technique for estimating herbage dry matter intake of dairy cows offered herbage harvested at two different stages of growth in summer and autumn

Publisher: Elsevier BV

Date: 2019

DOI: 10.1016/J.ANIFEEDSCI.2018.11.003

Sex and porcine somatotropin impact on variation in growth performance and back fat thickness.

Publisher: CSIRO Publishing

Date: 2005

DOI: 10.1071/EA04100

Abstract: Managing variation in carcass weight and composition is an enormous challenge for the pig producer. A means of improving growth performance and reducing back fat is porcine exogenous somatotropin (pST) treatment. It is possible that this technology may also reduce variation in these important growth and carcass parameters. This study was conducted to provide data on the effect of sex and pST on variation in growth and carcass characteristics. Sixteen on-farm studies involving 1495 pigs were conducted in various states within Australia. On each farm, groups of pigs were randomly assigned within sex to one of 2 management regimes (control or pST regime). The control regime consisted of feeding the typical finisher diet used on that farm while the pST regime involved feeding the typical grower diet used on the farm combined with daily injections of pST (5 mg/day). Data from in idual pigs were collated and the summary descriptive statistics derived for each treatment group on each farm were then analysed. Daily liveweight gain was lower and feed conversion ratio (FCR) and back fat at the P2 site were higher in gilts than in boars. The pST regime increased average mean daily gain and reduced feed intake, FCR and back fat at the P2 site. The coefficient of variation (CV) around live daily gain was lower in gilts than in boars (19.3 v 22.3% for gilts and boars, respectively, P .001) and in pigs treated with the pST regime (22.1 v. 19.9% for the control and pST regimes, respectively, P .001). The CV around liveweight was higher in the control boars than in any of the other treatment groups as indicated by the main (P .001) and interactive (P .001) effects of both sex and pST regime. The coefficient of variation around P2 was reduced by pST (16.2 v. 14.2%, P .001) but was not different between gilts and boars. There was an interaction (P = 0.025) between pST regime and sex with the average kurtosis for the P2 distribution positive in gilts treated with pST but slightly negative in all the other groups. The distributions were on average skewed to the right, particularly in gilts (P = 0.017) and in pigs treated with pST (P .001). In conclusion, these data demonstrate that as well as improving mean final liveweight and P2 back fat, pST treatment can also reduce variation in these key production parameters that form the basis for economic returns to the producer.

Heat Stress and Goat Welfare: Adaptation and Production Considerations

Publisher: MDPI AG

Date: 04-04-2021

DOI: 10.3390/ANI11041021

Abstract: This review attempted to collate and synthesize information on goat welfare and production constraints during heat stress exposure. Among the farm animals, goats arguably are considered the best-suited animals to survive in tropical climates. Heat stress was found to negatively influence growth, milk and meat production and compromised the immune response, thereby significantly reducing goats’ welfare under extensive conditions and transportation. Although considered extremely adapted to tropical climates, their production can be compromised to cope with heat stress. Therefore, information on goat adaptation and production performance during heat exposure could help assess their welfare. Such information would be valuable as the farming communities are often struggling in their efforts to assess animal welfare, especially in tropical regions. Broadly three aspects must be considered to ensure appropriate welfare in goats, and these include (i) housing and environment (ii) breeding and genetics and (iii) handling and transport. Apart from these, there are a few other negative welfare factors in goat rearing, which differ across the production system being followed. Such negative practices are predominant in extensive systems and include nutritional stress, limited supply of good quality water, climatic extremes, parasitic infestation and lameness, culminating in low production, reproduction and high mortality rates. Broadly two types of methodologies are available to assess welfare in goats in these systems: (i) animal-based measures include behavioral measurements, health and production records and disease symptoms (ii) resources based and management-based measures include stocking density, manpower, housing conditions and health plans. Goat welfare could be assessed based on several indicators covering behavioral, physical, physiological and productive responses. The important indicators of goat welfare include agonistic behavior, vocalization, skin temperature, body condition score (BCS), hair coat conditions, rectal temperature, respiration rate, heart rate, sweating, reduced growth, reduced milk production and reduced reproductive efficiency. There are also different approaches available by which the welfare of goats could be assessed, such as naturalistic, functional and subjective approaches. Thus, assessing welfare in goats at every production stage is a prerequisite for ensuring appropriate production in this all-important species to guarantee optimum returns to the marginal and subsistence farmers.

A short-term supranutritional vitamin E supplementation alleviated respiratory alkalosis but did not reduce oxidative stress in heat stressed pigs

Publisher: Asian Australasian Association of Animal Production Societies

Date: 02-2018

DOI: 10.5713/AJAS.17.0256

High-Throughput Screening and Characterization of Phenolic Compounds in Stone Fruits Waste by LC-ESI-QTOF-MS/MS and Their Potential Antioxidant Activities

Publisher: MDPI AG

Date: 04-02-2021

DOI: 10.3390/ANTIOX10020234

Abstract: Stone fruits, including peach (Prunus persica L.), nectarine (Prunus nucipersica L.), plum (Prunus domestica L.) and apricot (Prunus armeniaca L.) are common commercial fruits in the market. However, a huge amount of stone fruits waste is produced throughout the food supply chain during picking, handling, processing, packaging, storage, transportation, retailing and final consumption. These stone fruits waste contain high phenolic content which are the main contributors to the antioxidant potential and associated health benefits. The antioxidant results showed that plum waste contained higher concentrations of total phenolic content (TPC) (0.94 ± 0.07 mg gallic acid equivalents (GAE)/g) and total flavonoid content (TFC) (0.34 ± 0.01 mg quercetin equivalents (QE)/g), while apricot waste contained a higher concentration of total tannin content (TTC) (0.19 ± 0.03 mg catechin equivalents (CE)/g) and DPPH activity (1.47 ± 0.12 mg ascorbic acid equivalents (AAE)/g). However, nectarine waste had higher antioxidant capacity in ferric reducing-antioxidant power (FRAP) (0.98 ± 0.02 mg AAE/g) and total antioxidant capacity (TAC) (0.91 ± 0.09 mg AAE/g) assays, while peach waste showed higher antioxidant capacity in 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay (0.43 ± 0.09 mg AAE/g) as compared to other stone fruits waste. Qualitative and quantitative phenolic analysis of Australian grown stone fruits waste were conducted by liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS) and HPLC-photodiode array detection (PDA). The LC-ESI-QTOF-MS/MS result indicates that 59 phenolic compounds were tentatively characterized in peach (33 compounds), nectarine (28), plum (38) and apricot (23). The HPLC-PDA indicated that p-hydroxybenzoic acid (18.64 ± 1.30 mg/g) was detected to be the most dominant phenolic acid and quercetin (19.68 ± 1.38 mg/g) was the most significant flavonoid in stone fruits waste. Hence, it could be concluded that stone fruit waste contains various phenolic compounds and have antioxidant potential. The results could support the applications of these stone fruit wastes in other food, feed, nutraceutical and pharmaceutical industries.

Assessment of apparent ileal digestibility of amino acids and nitrogen in cottonseed and soyabean meals fed to pigs determined using ileal dissection under halothane anaesthesia or following carbon dioxide-stunning

Publisher: Cambridge University Press (CUP)

Date: 02-1998

DOI: 10.1017/S0007114598001093

Abstract: Two experiments were conducted to determine apparent ileal digestibility of amino acids (AIDAA) and nitrogen (AIDN) in cottonseed meal (CSM) and soyabean meal (SBM) fed to growing pigs. In the first experiment, twenty-four male pigs (37·3 (SE 2·7) kg) were in idually penned and randomized to either CSM or SBM diets. The diets contained 40% of the protein meal (either CSM or SBM) in a wheat starch-sucrose (1:1, w/w) base containing vitamins and minerals, and Cr 2 O 3 as an indigestible marker. Pigs were acclimated to the experimental diets over a 3d period and on day 4 through to day 14 were offered 1800g/d of the diet. Diets were offered in three meals/d from day 4 to day 11 and in eight meals/d from day 12 to day 13. After the eighth hourly-meal on day 14, twelve pigs were anaesthetized with halothane while the remaining twelve pigs were CO 2 -stunned and processed using commercial slaughter procedures. Ileal digesta were collected from a 1500 mm portion of the terminal ileum of each pig and subsequently analysed for amino acids, N, organic matter and Cr. Results indicated that AIDAA of CSM and SBM were lower when digesta were collected following CO 2 -stunning than when digesta were obtained under halothane anaesthesia. Consistently, AIDN in CSM (0·51 v. 0·56) and SBM (0·55 v. 0-71) were lower ( P ·05) in CO 2 -stunned pigs than in halothane-anaesthetized pigs. Furthermore, when digesta collection was conducted under halothane anaesthesia, AIDN of CSM was lower ( P 0·001) than that of SBM. In the second experiment, six male pigs (45 (SE 2·6) kg) were fitted with T-piece cannulas implanted in the terminal ileum, housed in idually in metabolism cages, and randomly allocated to either CSM or SBM diets in a single reversal arrangement. Ileal digesta were collected for AIDAA and AIDN determination. Although statistical comparisons could not be made between the two experiments, the AIDAA and AIDN data obtained via cannulated pigs were similar to those values obtained using the halothane-anaesthesia method. Overall, the CO 2 -stunning method is not recommended for studies of amino acid or nitrogen ileal digestibilities, but may be useful for the study of other dietary constituents.

Physiological Responses to Basic Tastes for Sensory Evaluation of Chocolate Using Biometric Techniques

Publisher: MDPI AG

Date: 05-07-2019

DOI: 10.3390/FOODS8070243

Abstract: Facial expressions are in reaction to basic tastes by the response to receptor stimulation. The objective of this study was to assess the autonomic nervous system responses to basic tastes in chocolates and to identify relationships between conscious and unconscious responses from participants. Panelists (n = 45) tasted five chocolates with either salt, citric acid, sugar, or monosodium glutamate, which generated four distinctive basic tastes plus bitter, using dark chocolate. An integrated camera system, coupled with the Bio-Sensory application, was used to capture infrared thermal images, videos, and sensory responses. Outputs were used to assess skin temperature (ST), facial expressions, and heart rate (HR) as physiological responses. Sensory responses and emotions elicited during the chocolate tasting were evaluated using the application. Results showed that the most liked was sweet chocolate (9.01), while the least liked was salty chocolate (3.61). There were significant differences for overall liking (p 0.05) but none for HR (p = 0.75) and ST (p = 0.27). Sweet chocolate was inversely associated with angry, and salty chocolate positively associated with sad. Positive emotion-terms were associated with sweet s les and liking in self-reported responses. Findings of this study may be used to assess novel tastes of chocolate in the industry based on conscious and emotional responses more objectively.

Effects of Imagery as Visual Stimuli on the Physiological and Emotional Responses

Publisher: MDPI AG

Date: 12-06-2019

DOI: 10.3390/J2020015

Abstract: Study of emotions has gained interest in the field of sensory and consumer research. Accurate information can be obtained by studying physiological behavior along with self-reported-responses. The aim was to identify physiological and self-reported-responses towards visual stimuli and predict self-reported-responses using biometrics. Panelists (N = 63) were exposed to 12 images (ten from Geneva Affective PicturE Database (GAPED), two based on common fears) and a questionnaire (Face scale and EsSense). Emotions from facial expressions (FaceReaderTM), heart rate (HR), systolic pressure (SP), diastolic pressure (DP), and skin temperature (ST) were analyzed. Multiple regression analysis was used to predict self-reported-responses based on biometrics. Results showed that physiological along with self-reported responses were able to separate images based on cluster analysis as positive, neutral, or negative according to GAPED classification. Emotional terms with high or low valence were predicted by a general linear regression model using biometrics, while calm, which is in the center of emotion dimensional model, was not predicted. After separating images, positive and neutral categories could predict all emotional terms, while negative predicted Happy, Sad, and Scared. Heart Rate predicted emotions in positive (R2 = 0.52 for Scared) and neutral (R2 = 0.55 for Sad) categories while ST in positive images (R2 = 0.55 for Sad, R2 = 0.45 for Calm).

Forms of n‐3 (ALA, C18:3n‐3 or DHA, C22:6n‐3) Fatty Acids Affect Carcass Yield, Blood Lipids, Muscle n‐3 Fatty Acids and Liver Gene Expression in Lambs

Publisher: Wiley

Date: 22-09-2015

DOI: 10.1007/S11745-015-4070-4

Abstract: The effects of supplementing diets with n-3 alpha-linolenic acid (ALA) and docosahexaenoic acid (DHA) on plasma metabolites, carcass yield, muscle n-3 fatty acids and liver messenger RNA (mRNA) in lambs were investigated. Lambs (n = 120) were stratified to 12 groups based on body weight (35 ± 3.1 kg), and within groups randomly allocated to four dietary treatments: basal diet (BAS), BAS with 10.7 % flaxseed supplement (Flax), BAS with 1.8 % algae supplement (DHA), BAS with Flax and DHA (FlaxDHA). Lambs were fed for 56 days. Blood s les were collected on day 0 and day 56, and plasma analysed for insulin and lipids. Lambs were slaughtered, and carcass traits measured. At 30 min and 24 h, liver and muscle s les, respectively, were collected for determination of mRNA (FADS1, FADS2, CPT1A, ACOX1) and fatty acid composition. Lambs fed Flax had higher plasma triacylglycerol, body weight, body fat and carcass yield compared with the BAS group (P < 0.001). DHA supplementation increased carcass yield and muscle DHA while lowering plasma insulin compared with the BAS diet (P < 0.01). Flax treatment increased (P < 0.001) muscle ALA concentration, while DHA treatment increased (P < 0.001) muscle DHA concentration. Liver mRNA FADS2 was higher and CPT1A lower in the DHA group (P < 0.05). The FlaxDHA diet had additive effects, including higher FADS1 and ACOX1 mRNA than for the Flax or DHA diet. In summary, supplementation with ALA or DHA modulated plasma metabolites, muscle DHA, body fat and liver gene expression differently.

Effects of oxytocin administration on the response of piglets to weaning

Publisher: MDPI AG

Date: 16-07-2015

DOI: 10.3390/ANI5030371

Betaine and Antioxidants Improve Growth Performance, Breast Muscle Development and Ameliorate Thermoregulatory Responses to Cyclic Heat Exposure in Broiler Chickens

Publisher: MDPI AG

Date: 25-09-2018

DOI: 10.3390/ANI8100162

Abstract: Heat stress (HS) is an environmental stressor challenging poultry production and requires a strategy to cope with it. A total of 288-day-old male broiler chicks were fed with one of the following diets: basal diet, basal with betaine (BET), or with selenium and vitamin E (AOX), or with a combination of BET and AOX, under thermoneutral and cyclic HS. Results showed that HS reduced average daily feed intake (ADFI) (p = 0.01) and average daily gain (ADG) (p 0.001), and impaired feed conversion ratio (FCR) (p = 0.03) during rearing period (0–42 day). BET increased ADG (p = 0.001) and decreased FCR (p = 0.02), whereas AOX had no effects. Breast muscle weight was decreased by HS (p 0.001) and increased by BET (p 0.001). Rectal temperature was increased by HS (p 0.001) and improved by BET overall. Respiration rate was increased by HS (p 0.001), but BET decreased it during HS (p = 0.04). Jejunum transepithelial resistance was reduced by HS and had no effect on permeability whereas BET increased jejunum permeability (p = 0.013). Overall, the reductions in ADG of broiler chickens during HS were ameliorated by supplementation with BET, with much of the increase in ADG being breast muscle.

Diets containing high-quality animal proteins increase growth of early-weaned pigs

Publisher: No publisher found

Date: 2002

DOI: 10.1071/AR01191

Plant bioactives for ruminant health and productivity

Publisher: Elsevier BV

Date: 2008

DOI: 10.1016/J.PHYTOCHEM.2007.08.017

Abstract: Plants have been used throughout history for their medicinal properties. This use has often focused on human health but plants have also been, and still are, applied in ethnoveterinary practice and animal health management. In recent times, the use of synthetic chemicals has become prevalent. Public awareness of the potential environmental and health risks associated with heavy chemical use has also increased. This has put pressure on regulatory bodies to reduce the use of chemicals in agriculture. The most striking ex le is the 2006 banning of antibiotics in animal feed by the European Union. Moves such as this have increased the drive to find alternatives to synthetic chemicals and research has again turned to the use of plant bioactives as a means of improving animal health. Current scientific evidence suggests there is significant potential to use plants to enhance animal health in general and that of ruminants (cattle, deer, sheep, etc.) in particular. Active areas of research for plant bioactives (particularly saponin and tannin containing plants) include reproductive efficiency, milk and meat quality improvement, foam production/bloat control and methane production. Nematode control is also a significant area of research and the evidence suggests a much broader range of phytochemicals may be effective. This review presents a summary of the literature and examines international research efforts towards the development of plant bioactives for animal health.

Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolaemic pigs on high- and low-fat diets

Publisher: Cambridge University Press (CUP)

Date: 10-05-2007

DOI: 10.1017/S0007114507747803

Interactions between weaning age, weaning weight, sex, and enzyme supplementation on growth performance of pigs

Publisher: CSIRO Publishing

Date: 2002

DOI: 10.1071/AR01197

Abstract: Digestive capacity of early-weaned pigs may be insufficient to fully digest many ingredients currently used in weaner diets. The aim of this experiment was to determine whether an exogenous enzyme preparation with broad carbohydrase activity could benefit pigs that developmentally might be immature at weaning, especially with regard to gastrointestinal development. Eighty Large White × Landrace pigs were used in a 2 × 2 × 2 × 2 factorial experiment with the factors being: weaning age (14 or 24 days), weaning weight (heavy or light), sex (boar or gilt), and dietary Biofeed Plus CT (0 or 500 μg/g) which contained fungal xylanases, pentosanases and β-glucanases. Pigs were housed in idually and given a wheat-based (550 g/kg) diet containing 15.0 MJ DE and 15.9 g lysine/kg on an ad libitum basis for 21 days. The diet also contained 50 g/kg of soybean meal and 50 g/kg of lupin (Lupinus�angustifolius) kernels. The liveweights of heavy (H) pigs weaned at 24 or 14 days and light pigs (L) weaned at 24 or 14 days were 7.9 and 5.3 and 5.2 and 3.9 kg, respectively. Pigs weaned at 14 days grew slower (157 v. 345 g/day) than those weaned at 24 days, although there was a suggestion of an interaction between age and weight at weaning (P = 0.081). Thus, H and L pigs weaned at 14 days grew at 148 and 166 g/day, whereas H and L pigs weaned at 24 days grew at 374 and 315 g/day, respectively. Although there was no main effect of enzymes on daily gain (248 v. 254 g/day, P = 0.80), feed intake (278 v. 284 g/day, P = 0.79), or feed conversion ratio (1.19 v. 1.25, P�=�0.35), there were interactions with weaning age on daily gain (P = 0.050) and feed intake (P = 0.060). Pigs weaned at 14 days grew slower (176 v. 138 g/day) and ate less (206 v. 174 g/day), whereas pigs weaned at 24 days grew faster (321 v. 369 g/day) and ate more (351 v. 394 g/day), when supplemented with enzymes. During the third week after weaning there were interactions between dietary enzymes and sex (P = 0.060) and dietary enzymes and age (P = 0.023) on daily gain. Thus, pigs weaned at 24 days and supplemented with Biofeed Plus CT grew more quickly during the third week (559 v. 460 g/day), whereas the converse was true for pigs weaned at 14 days (286 v. 334 g/day). Also, enzyme-supplemented boars grew better over this period (457 v. 371 g/day), whereas the converse was true for gilts (388 v. 423 g/day). In conclusion, these data clearly indicate that the greatest determinant of post-weaning performance under the present conditions was the age of the pigs at weaning. Dietary enzyme supplementation appeared most efficacious in boars weaned at an older age, although benefits did not become apparent until 2 weeks after weaning.

Comparison of silver‐ion high‐performance liquid chromatographic quantification of free and methylated conjugated linoleic acids

Publisher: Wiley

Date: 10-2000

DOI: 10.1007/S11745-000-0630-Y

Abstract: Silver-ion high-performance liquid chromatography was used to fractionate a mixture of conjugated linoleic acid (CLA) isomers (as the free fatty acids, CLAFFA) in commercial CLA mixtures and biological s les. Due to the unchanged retention mechanism, it was assumed that the elution order of the isomers remained the same as that of methyl esters separated on the same column. The most abundant isomers, cis/trans 10, 12-18:2 and cis/trans 9,11-18:2, were separated better as free acids on a single column than in the methyl ester form. Quantification of the CLA standard was used as the reference profile to evaluate different methylation methods commonly used to prepare CLA methyl esters for quantitation. Acid-and base-catalyzed derivatization methods resulted in CLA intraisomerization and losses in total conjugated dienes content. Acid (HCl and BF3) methylations significantly elevated the level of trans,trans isomers and significantly reduced the cis/trans isomers. Base methylation, tetramethylguanidine/methanol, resulted in loss of trans,trans isomers, and a substantial loss of total underivatized conjugated dienes. Other catalysts such as the trimethylsilyldiazomethane produced additional peaks of unidentified artifacts. The analysis of CLAFFA appears to provide more accurate quantification of CLA isomers in commercial and biological s les.

A dietary sugarcane-derived polyphenol mix reduces the negative effects of cyclic heat exposure on growth performance, blood gas status, and meat quality in broiler chickens

Publisher: MDPI AG

Date: 08-07-2020

DOI: 10.3390/ANI10071158

Abstract: Heat stress (HS) compromises growth performance and meat quality of broiler chickens by interrupting lipid and protein metabolism, resulting in increased oxidative damages. The experiment attempted to investigate whether dietary polyphenols (Polygain (POL)) could ameliorate the aforementioned adverse effects of HS on performance and meat quality. One hundred and twenty one day-old-male chicks were allocated to two temperature conditions, thermoneutral (TN) or HS, and fed with either a control diet (CON) or the CON plus four different doses of POL (2, 4, 6 and 10 g/kg). Heat stress caused respiratory alkalosis as evidenced by increased rectal temperature (p 0.001) and respiration rate (p 0.001) due to increased blood pH (p 0.001). Heat stress decreased final body weight (p = 0.061) and breast muscle water content (p = 0.013) while POL improved both (p = 0.002 and p = 0.003, respectively). Heat stress lified muscle damages, indicated by increased thiobarbituric acid reactive substances (p 0.001) and reduced myofibril fragmentation index (p = 0.006) whereas POL improved both (p = 0.037 and p = 0.092, respectively). Heat stress impaired meat tenderness (p 0.001) while POL improved it (p = 0.003). In conclusion, HS impaired growth performance and meat quality whereas POL ameliorated these responses in a dose-dependent manner, and effects of POL were evident under both temperature conditions.

Phenolic Compounds Profiling and Their Antioxidant Capacity in the Peel, Pulp, and Seed of Australian Grown Avocado

Publisher: MDPI AG

Date: 12-01-2023

DOI: 10.3390/ANTIOX12010185

Abstract: Avocados (Persea americana M.) are highly valued fruits consumed worldwide, and there are numerous commercially available varieties on the market. However, the high demand for fruit also results in increased food waste. Thus, this study was conducted for comprehensive profiling of polyphenols of Hass, Reed, and Wurtz avocados obtained from the Australian local market. Ripe Hass peel recorded the highest TPC (77.85 mg GAE/g), TTC (148.98 mg CE/g), DPPH (71.03 mg AAE/g), FRAP (3.05 mg AAE/g), RPA (24.45 mg AAE/g), and ABTS (75.77 mg AAE/g) values unripe Hass peel recorded the highest TFC (3.44 mg QE/g) and Wurtz peel recorded the highest TAC (35.02 mg AAE/g). Correlation analysis revealed that TPC and TTC were significantly correlated with the antioxidant capacity of the extracts. A total of 348 polyphenols were screened in the peel. A total of 134 compounds including 36 phenolic acids, 70 flavonoids, 11 lignans, 2 stilbenes, and another 15 polyphenols, were characterised through LC-ESI-QTOF-MS/MS, where the majority were from peels and seeds of s les extract. Overall, the hierarchical heat map revealed that there were a significant amount of polyphenols in peels and seeds. Epicatechin, kaempferol, and protocatechuic acid showed higher concentrations in Reed pulp. Wurtz peel contains a higher concentration of hydroxybenzoic acid. Our results showed that avocado wastes have a considerable amount of polyphenols, exhibiting antioxidant activities. Each s le has its unique value proposition based on its phenolic profile. This study may increase confidence in utilising by-products and encourage further investigation into avocado by-products as nutraceuticals.

Reducing the lysine to energy content in the grower growth phase diet increases intramuscular fat and improves the eating quality of the longissimus thoracis muscle of gilts

Publisher: CSIRO Publishing

Date: 2008

DOI: 10.1071/EA07287

Abstract: Sixty-three (Large White × Landrace × Duroc) female finisher pigs were used to determine the effect of nutritional manipulations on growth performance, carcass quality and sensory pork quality. The dietary treatments were: (i) control [pigs fed a commercial grower (73–125 days of age) and finisher diet (126–163 days of age)] (ii) 15% reduced lysine : energy diet during the grower phase followed by a commercial finisher diet and (iii) pigs fed the control diet with no supplemented vitamin A in the mineral–vitamin premix during both the grower and finisher phases. Pigs fed the reduced lysine diet had higher (P 0.05) intramuscular fat levels compared with pigs fed the control and the vitamin A- restricted diets. The pork from pigs fed the reduced lysine diet had a lower ultimate muscle pH, was paler (higher L*) and had a higher b* value (yellowness-blueness) compared with pork from the control pigs (all P 0.05). Pork from pigs fed the reduced lysine diet was considered to be the most juicy and tender and have the best overall acceptability (all P 0.05), followed by pork from pigs fed the control and vitamin A-restricted diets. In conclusion, feeding pigs a 15% reduced lysine : energy diet significantly increased intramuscular fat levels and improved the sensory quality of pork.

Seasonal variation in the concentrations of conjugated linoleic and trans fatty acids in milk fat from commercial dairy farms is associated with pasture and grazing management and supplementary feedin

Publisher: CSIRO Publishing

Date: 2008

DOI: 10.1071/EA07286

Abstract: A study of irrigated pasture-based dairy farms that used split calving (autumn and spring) was undertaken in northern Victoria, Australia, to examine associations between nutrition, time of year and season of calving on the concentrations of isomers of trans 18 : 1 fatty acids and conjugated linoleic acids (CLA) in milk fat. Factors associated with time of year explained most of the variation, with the highest concentrations observed in spring and summer when pasture intake by herds was high. However, there was substantial variation observed between herds and time of year. The mean total CLA concentration was 9.1 mg/g milk fatty acids (range 1.1–35.4 mg/g) with the cis,trans-9,11 accounting for ~84% of the total CLA. The mean total trans 18 : 1 concentration was 60.5 mg/g milk fatty acids (range 13.6–267 mg/g) with vaccenic acid (trans-11 18 : 1) accounting for ~53% of total trans 18 : 1 fatty acids. Total CLA and vaccenic acid were highest in August–September (southern hemisphere spring) (15.1 and 76.3 mg/g milk fat) and lowest in November–March (5.6 mg/g milk fat) and May–July (9.53 mg/g milk fat), respectively. There was no association between season of calving and milk CLA or trans 18 : 1 fatty acid concentrations. Trans-10 and -11 18 : 1 fatty acids and trans/trans-CLA were negatively correlated with milk fat concentrations. Management strategies designed to increase the concentration of CLA and trans 18 : 1 fatty acids in milk fat would not need to consider the effects of season of calving or stage of lactation, but should focus on pasture availability and quality.

Non-Invasive Sheep Biometrics Obtained by Computer Vision Algorithms and Machine Learning Modeling Using Integrated Visible/Infrared Thermal Cameras

Publisher: MDPI AG

Date: 06-11-2020

DOI: 10.3390/S20216334

Abstract: Live sheep export has become a public concern. This study aimed to test a non-contact biometric system based on artificial intelligence to assess heat stress of sheep to be potentially used as automated animal welfare assessment in farms and while in transport. Skin temperature (°C) from head features were extracted from infrared thermal videos (IRTV) using automated tracking algorithms. Two parameter engineering procedures from RGB videos were performed to assess Heart Rate (HR) in beats per minute (BPM) and respiration rate (RR) in breaths per minute (BrPM): (i) using changes in luminosity of the green (G) channel and (ii) changes in the green to red (a) from the CIELAB color scale. A supervised machine learning (ML) classification model was developed using raw RR parameters as inputs to classify cutoff frequencies for low, medium, and high respiration rate (Model 1). A supervised ML regression model was developed using raw HR and RR parameters from Model 1 (Model 2). Results showed that Models 1 and 2 were highly accurate in the estimation of RR frequency level with 96% overall accuracy (Model 1), and HR and RR with R = 0.94 and slope = 0.76 (Model 2) without statistical signs of overfitting

Dietary betaine and ractopamine combine to increase lean tissue deposition in finisher pigs, particularly gilts

Publisher: CSIRO Publishing

Date: 2009

DOI: 10.1071/EA08014

Abstract: Dietary ractopamine increases lean tissue deposition, with the responses most evident at ad libitum feed intakes whereas dietary betaine can improve growth by reducing maintenance requirements, with the greatest responses occurring when energy is limiting. This study was conducted to investigate the interactions between dietary ractopamine and betaine on growth and carcass characteristics in restrictively fed [31.0 MJ digestible energy (DE)/day for 14 days followed by 36.8 MJ DE/day for 21 days] boars and gilts. Forty in idually penned pigs (58.4 kg) were allocated to a 2 × 2 × 2 factorial arrangement of treatments, with the respective factors being sex (gilt or boar), dietary betaine (0 and 1.5 g/kg betaine) and dietary ractopamine (0 and 10 ppm ractopamine) for 35 days. Over the first 14 days of the study when the restriction was greatest, daily gain was greater in pigs fed betaine (+8%, P = 0.04) and in boars (+12%, P = 0.005) but was not affected by ractopamine (P = 0.18). Lean deposition was greater in pigs fed betaine (+5%, P = 0.08) and in boars (+6%, P = 0.006) but was not affected by ractopamine (P = 0.57). However, there was an interaction (P = 0.03) between ractopamine and sex such that ractopamine increased lean deposition in gilts but not boars. Thus, betaine and ractopamine had additive effects on lean mass in gilts (+5.1 kg) but not boars. Fat deposition was less in pigs fed ractopamine (–8%, P = 0.05) and in boars (–17%, P 0.001) but was not affected by betaine (P = 0.81). However, there was an interaction (P = 0.04) between dietary ractopamine and sex such that ractopamine decreased fat deposition in gilts (–14%) but not boars. In conclusion, dietary betaine and ractopamine may have additive effects on lean deposition and improve body composition in gilts but responses in boars are more equivocal.

Development of Artificial Neural Network Models to Assess Beer Acceptability Based on Sensory Properties Using a Robotic Pourer: A Comparative Model Approach to Achieve an Artificial Intelligence System

Publisher: MDPI AG

Date: 05-2019

DOI: 10.3390/BEVERAGES5020033

Abstract: Artificial neural networks (ANN) have become popular for optimization and prediction of parameters in foods, beverages, agriculture and medicine. For brewing, they have been explored to develop rapid methods to assess product quality and acceptability. Different beers (N = 17) were analyzed in triplicates using a robotic pourer, RoboBEER (University of Melbourne, Melbourne, Australia), to assess 15 color and foam-related parameters using computer-vision. Those s les were tested using sensory analysis for acceptability of carbonation mouthfeel, bitterness, flavor and overall liking with 30 consumers using a 9-point hedonic scale. ANN models were developed using 17 different training algorithms with 15 color and foam-related parameters as inputs and liking of four descriptors obtained from consumers as targets. Each algorithm was tested using five, seven and ten neurons and compared to select the best model based on correlation coefficients, slope and performance (mean squared error (MSE). Bayesian Regularization algorithm with seven neurons presented the best correlation (R = 0.98) and highest performance (MSE = 0.03) with no overfitting. These models may be used as a cost-effective method for fast-screening of beers during processing to assess acceptability more efficiently. The use of RoboBEER, computer-vision algorithms and ANN will allow the implementation of an artificial intelligence system for the brewing industry to assess its effectiveness.

Characterization of Phenolics in Rejected Kiwifruit and Their Antioxidant Potential

Publisher: MDPI AG

Date: 28-04-2021

DOI: 10.3390/PR9050781

Abstract: Kiwifruit hold significant nutritional value and are a good source of antioxidants due to their erse range of bioactive compounds. Kiwifruit waste is generated throughout the food supply chain, particularly during transportation and storage. Kiwifruit rejected from the retail market due to unfavorable appearance still possess potential economic value as kiwifruit are abundant in phenolic compounds. The present work studied the phenolic profile and antioxidant potential of rejected kiwifruit, including SunGold (Actinidia chinensis), Hayward (Actinidia deliciosa), and round organic Hayward (Actinidia deliciosa). Regarding phenolics estimation, SunGold possessed the highest TPC (0.72 ± 0.01 mg GAE/g), while Hayward exhibited the highest TFC (0.05 ± 0.09 mg QE/g). In antioxidant assays, SunGold showed the highest antioxidant activities in DPPH (0.31 ± 0.35 mg AAE/g), FRAP (0.48 ± 0.04 mg AAE/g), ABTS (0.69 ± 0.07 mg AAE/g), •OH-RSA (0.07 ± 0.03 mg AAE/g) assays, and FICA (0.19 ± 0.07 mg EDTA/g), whereas Hayward showed the highest RPA (0.09 ± 0.02 mg AAE/g) and TAC (0.57 ± 0.04 mg AAE/g). Separation and characterization of phenolics were conducted using LC-ESI-QTOF-MS/MS. A total of 97 phenolics were tentatively characterized from rejected SunGold (71 phenolics), Hayward (55 phenolics), and round organic Hayward (9 phenolics). Hydroxycinnamic acids and flavonols were the most common phenolics characterized in the three s les. The quantitative analysis was conducted by HPLC-PDA and found that chlorogenic acid (23.98 ± 0.95 mg/g), catechin (23.24 ± 1.16 mg/g), and quercetin (24.59 ± 1.23 mg/g) were the most abundant phenolics present in the rejected kiwifruit s les. The notable presence of phenolic compounds and their corresponding antioxidant capacities indicate the potential value of rescuing rejected kiwifruit for further utilization and commercial exploitation.

Effects of Feeding a Commercial Starch Binding Agent during Heat Stress on Enteric Methane Emission, Rumen Volatile Fatty Acid Contents, and Diet Digestibility of Merino Lambs

Publisher: MDPI AG

Date: 22-03-2023

DOI: 10.3390/ATMOS14030605

Abstract: Twenty-four Merino lambs were allocated to three dietary treatment groups to determine the effects of a dietary starch and protein binding agent and heat stress on methane (CH4) emissions and rumen parameters. The diets were a wheat-based diet (WD), a 2% Bioprotect™ (Bioprotect™, RealisticAgri, Rutland, UK) treated wheat-based diet (BD), and a maize-based diet (MD) for 3 periods of 1-week duration. During Period 1 (P1) the lambs were maintained under thermoneutral conditions and at a 1.7 × Maintenance (M) level. During P2 and P3, the lambs were maintained under cyclic heat stress conditions and fed at 1.7 × M and 2.0 × M, respectively. Total CH4 production was lower for the BD diet than the WD diet, which in turn was lower than the MD diet (p 0.001). Total CH4 production was lower during P2 than P1 with P3 intermediate (p = 0.04). Rumen total volatile fatty acid (TVFA) concentrations were higher for the WD diet than the MD diet with the BD diet intermediate (p = 0.01). Rumen TVFA concentrations were lower during heat stress than under thermoneutral conditions (p 0.001). Whole tract starch digestibility was higher for the BD and WD diets than the MD diet (p 0.001). In conclusion, feeding Merino lambs the BD diet reduces CH4 emissions without reducing starch digestibility.

Selenium levels in cows fed pasture and concentrates or a total mixed ration and supplemented with selenized yeast to produce milk with supra-nutritional selenium concentrations

Publisher: American Dairy Science Association

Date: 2011

DOI: 10.3168/JDS.2010-3590

Abstract: Seventy multiparous Holstein-Friesian cows were fed different amounts of pasture and concentrates, or a total mixed ration (TMR), for 42 d in mid-lactation to test the hypothesis that the concentration of Se in milk would depend on the amount of Se consumed, when the Se is primarily organic in nature, regardless of the diet of the cows. Of the 70 cows, 60 grazed irrigated perennial pasture at daily allowances of either 20 or 40 kg of dry matter (DM)/cow. These cows received 1 of 3 amounts of concentrates, either 1, 3, or 6 kg of DM/cow per day of pellets, and at each level of concentrate feeding, the pellets were formulated to provide 1 of 2 quantities of Se from Se yeast, either about 16 or 32 mg of Se/d. The other 10 cows were included in 2 additional treatments where a TMR diet was supplemented with 1 kg of DM/d of pellets formulated to include 1 of the 2 quantities of supplemental Se. Total Se intakes ranged from 14.5 to 35.9 mg/d, and of this, the Se-enriched pellets provided 93, 91, and 72% of the Se for cows allocated 20 and 40 kg of pasture DM/d or the TMR, respectively. No effects of the amount of Se consumed on any milk production variable, or on somatic cell count, body weight, and body condition score, for either the pasture-fed or TMR-fed cows were found. Milk Se concentrations responded quickly to the commencement of Se supplementation, reaching 89% of steady state levels at d 5. When milk Se concentrations were at steady state (d 12 to 40), each 1mg of Se eaten increased the Se concentration of milk by 5.0 μg/kg (R(2)=0.97), and this response did not seem to be affected by the diet of the cows or their milk production. The concentration of Se in whole blood was more variable than that in milk, and took much longer to respond to change in Se status, but it was not affected by diet at any time either. For the on-farm production of Se-enriched milk, it is important to be able to predict milk Se concentration from Se input. In our study, type of diet did not affect this relationship.

Livestock Identification Using Deep Learning for Traceability

Publisher: MDPI AG

Date: 28-10-2022

DOI: 10.3390/S22218256

Abstract: Farm livestock identification and welfare assessment using non-invasive digital technology have gained interest in agriculture in the last decade, especially for accurate traceability. This study aimed to develop a face recognition system for dairy farm cows using advanced deep-learning models and computer vision techniques. This approach is non-invasive and potentially applicable to other farm animals of importance for identification and welfare assessment. The video analysis pipeline follows standard human face recognition systems made of four significant steps: (i) face detection, (ii) face cropping, (iii) face encoding, and (iv) face lookup. Three deep learning (DL) models were used within the analysis pipeline: (i) face detector, (ii) landmark predictor, and (iii) face encoder. All DL models were finetuned through transfer learning on a dairy cow dataset collected from a robotic dairy farm located in the Dookie c us at The University of Melbourne, Australia. Results showed that the accuracy across videos from 89 different dairy cows achieved an overall accuracy of 84%. The computer program developed may be deployed on edge devices, and it was tested on NVIDIA Jetson Nano board with a camera stream. Furthermore, it could be integrated into welfare assessment previously developed by our research group.

Probiotic Yoghurt Enriched with Mango Peel Powder: Biotransformation of Phenolics and Modulation of Metabolomic Outputs after In Vitro Digestion and Colonic Fermentation

Publisher: MDPI AG

Date: 10-05-2023

DOI: 10.3390/IJMS24108560

Abstract: This study investigated the health-promoting effects and prebiotic functions of mango peel powder (MPP) both as a plain in idual ingredient and when incorporated in yoghurt during simulated digestion and fermentation. The treatments included plain MPP, plain yoghurt (YA), yoghurt fortified with MPP (YB), and yoghurt fortified with MPP and lactic acid bacteria (YC), along with a blank (BL). The identification of polyphenols in the extracts of insoluble digesta and phenolic metabolites after the in vitro colonic fermentation were performed employing LC-ESI-QTOF-MS2. These extracts were also subjected to pH, microbial count, production of SCFA, and 16S rRNA analyses. The characterisation of phenolic profiles identified 62 phenolic compounds. Among these compounds, phenolic acids were the major compounds that underwent biotransformation via catabolic pathways such as ring fission, decarboxylation, and dehydroxylation. Changes in pH indicated that YC and MPP reduced the media pH from 6.27 and 6.33 to 4.50 and 4.53, respectively. This decline in pH was associated with significant increases in the LAB counts of these s les. The Bifidobacteria counts were 8.11 ± 0.89 and 8.02 ± 1.01 log CFU/g in YC and MPP, respectively, after 72 h of colonic fermentation. Results also showed that the presence of MPP imparted significant variations in the contents and profiles of in idual short chain fatty acids (SCFA) with more predominant production of most SCFA in the MPP and YC treatments. The 16s rRNA sequencing data indicated a highly distinctive microbial population associated with YC in terms of relative abundance. These findings suggested MPP as a promising ingredient for utilisation in functional food formulations aiming to enhance gut health.

Impact of Heatwaves on the Physiology and Retail Meat Quality of Lambs

Publisher: MDPI AG

Date: 31-01-2022

DOI: 10.3390/FOODS11030414

Abstract: The experiment investigated the impact of heatwaves (HWs) on the physiology, postmortem muscle metabolism and meat quality of lambs. Seventy-two second-cross lambs (Poll Dorset × (Border Leicester × Merino)) were selected and exposed to either 1, 3 or 5 days of HWs or thermoneutal (TN) (28–38 °C and 40–60% relative humidity, RH 18–21 °C, 40–55% RH) conditions in climate-controlled chambers. Lambs exposed to 1–5 days of a HW exhibited higher respiration rates (RRs), rectal temperatures (RTs), skin temperatures (STs) and heart rates (HRs) compared to lambs exposed to an equal duration of TN conditions. However, HWs had no significant effects on muscle metabolism (rate and extent of pH decline, muscle glycogen and lactate content) and meat quality (cooking loss and shear force). Similarly, there were limited impacts of 1–5 days of HW on the colour (L*, a*, b* and R630/580) and drip loss of the longissimus thoracis et lumborum (LTL) and semimembranosus (SM) muscles for 4 days’ overwrap retail display. Results suggest that short-duration HWs (1–5 days) had significant negative effects on animal physiology but had no effect on the muscle metabolism and meat quality.

LC-ESI-QTOF/MS Characterization of Phenolic Compounds from Medicinal Plants (Hops and Juniper Berries) and Their Antioxidant Activity

Publisher: MDPI AG

Date: 20-12-2019

DOI: 10.3390/FOODS9010007

Abstract: Hops (Humulus lupulus L.) and juniper berries (Juniperus communis L.) are two important medicinal plants widely used in the food, beverage, and pharmaceutical industries due to their strong antioxidant capacity, which is attributed to the presence of polyphenols. The present study is conducted to comprehensively characterize polyphenols from hops and juniper berries using liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS) to assess their antioxidant capacity. For polyphenol estimation, total phenolic content, flavonoids and tannins were measured, while for antioxidant capacity, three different antioxidant assays including the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay, the 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical cation decolorization assay and the ferric reducing-antioxidant power (FRAP) assay were used. Hops presented the higher phenolic content (23.11 ± 0.03 mg/g dw) which corresponded to its strong antioxidant activity as compared to the juniper berries. Using LC-ESI-QTOF/MS, a total of 148 phenolic compounds were tentatively identified in juniper and hops, among which phenolic acids (including hydroxybenzoic acids, hydroxycinnamic acids and hydroxyphenylpropanoic acids) and flavonoids (mainly anthocyanins, flavones, flavonols, and isoflavonoids) were the main polyphenols, which may contribute to their antioxidant capacity. Furthermore, the HPLC quantitative analysis showed that both s les had a high concentration of phenolic acids and flavonoids. In the HPLC quantification, the predominant phenolic acids in hops and juniper berries were chlorogenic acid (16.48 ± 0.03 mg/g dw) and protocatechuic acid (11.46 ± 0.03 mg/g dw), respectively. The obtained results highlight the importance of hops and juniper berries as a rich source of functional ingredients in different food, beverage, and pharmaceutical industries.

Phytochemistry, Bioaccessibility, and Bioactivities of Sesame Seeds: An Overview

Publisher: Informa UK Limited

Date: 20-01-2023

DOI: 10.1080/87559129.2023.2168280

Dietary lysine requirements of heavy and light pigs weaned at 14 days of age

Publisher: CSIRO Publishing

Date: 2000

DOI: 10.1071/AR99162

Abstract: Seventy 14-day-old male pigs that were either heavy (6.0 kg) or light (3.6 kg) for age were weaned into in idual pens and fed 1 of 7 diets containing 9.2–21.0 g lysine/kg feed in a study designed to determine the effect of dietary lysine and weight on lysine requirements of pigs. Five pigs from each weight group were used to determine initial body composition. When pigs reached 10 kg they were slaughtered and empty body composition was analysed to determine protein tissue accretion rates. Protein deposition rate was not affected by weight at weaning but increased with dietary lysine content before reaching a plateau. The relationship between protein deposition (PD, g/day) and dietary lysine (L, g/kg) was described by 3 models. The rectilinear model, which had a linear ascending phase (PD = 4.84 + 1.948L, R2 = 0.935, P = 0.002) and a horizontal component representing maximum protein deposition rate of 32.3 g/day, revealed that maximum protein deposition occurred at 14.1 g lysine/kg. The quadratic function (PD = – 2.74 + 3.74L – 0.099L2 , R2 = 0.916, P = 0.003) provided an estimate of the lysine requirement of 14.9 g lysine/kg occurring at a point where PD reached 95% of the maximum protein deposition rate (32.5 g/day). Use of an asymptotic model (PD = 32.60 – 186 0.727L, R2 = 0.919, P = 0.003) provided an estimate of 14.9 g lysine/kg occurring at a point where PD reached 95% of the maximum protein deposition rate (32.6 g/day). These data indicate that early weaned pigs should be fed a highly digestible diet containing 14–15 g lysine/kg to maximise protein deposition.

Differences in Thermoregulatory Responses between Dorper and Second Cross Lambs to Heat Stress Challenges

Publisher: MDPI

Date: 07-04-2020

DOI: 10.3390/PROCEEDINGS2019036155

Phytochemicals, Antioxidant Activities, and Toxicological Screening of Native Australian Fruits Using Zebrafish Embryonic Model

Publisher: MDPI AG

Date: 14-12-2022

DOI: 10.3390/FOODS11244038

Abstract: Phytochemicals play a pivotal role in human health and drug discovery. The safety evaluation of plant extracts is a prerequisite to ensure that all phytochemicals are safe before translational development and human exposure. As phytochemicals are natural, they are generally considered safe, although this is not always true. The objective of this study was to investigate and compare the phytochemical composition, antioxidant potential, and safety evaluation of native Australian Muntries (Kunzea pomifera), Kakadu plum (Terminalia ferdinandiana), Davidson plum (Davidsonia) and Quandong peach (Santalum acuminatum) through the in vivo vertebrate zebrafish embryonic model. The highest total phenolic content (TPC 793.89 ± 22.27 μg GAE/mg) was quantified in Kakadu plum, while the lowest TPC (614.44 ± 31.80 μg GAE/mg) was quantified in Muntries. Developmental alterations, mortality, and morbidity were assessed for toxicological screening of these selected native Australian fruit extracts. In this study, muntries were quantified as having the least LC50 value (169 mg/L) compared to Davidson plum (376 mg/L), Kakadu plum ( mg/L), and Quandong peach ( mg/L), which indicates that muntries extract was more toxic than other fruit extracts. Importantly, we found that adverse effects were not correlated to the total phenolic content and antioxidant potential of these native Australian fruits and cannot simply be predicted from the in vitro analysis. Conclusively, these selected native Australian fruit extracts are categorized as safe. This study could explore the use of these native Australian fruits in cosmetics, pharmaceuticals, and drug discovery.

Factors affecting the concentration of short branched-chain fatty acids in sheep fat

Publisher: CSIRO Publishing

Date: 2007

DOI: 10.1071/EA07037

Abstract: The concentration of short branched-chain fatty acids, specifically 4-methyloctanoic acid (MOA) and 4-methylnonanoic acid (MNA), in subcutaneous fat were determined in five genotypes (Poll Dorset × Border Leicester Merino, Poll Dorset × Merino, Poll Dorsetmuscling × Merino, Merino × Merino, Border Leicester × Merino) of Australian sheep. In total, 264 ewes and wethers, either 8 or 22 months of age, were selected for genotype, age and gender. These animals were sired by 16 rams that were selected for growth based on estimated breeding values (EBVs), except for one group (Poll Dorsetmuscling × Merino), which was selected for muscling based on EBVs. There was a strong relationship between the concentration of MOA and MNA at 8 and 22 months of age. There were no differences between lambs sired by Poll Dorset rams selected for muscling or growth. The 8- and 22-month-old Poll Dorset × Merino sheep had higher levels of MOA and MNA compared with the other genotypes (P 0.001). At 8 months of age, the wethers had higher levels of MOA and MNA (P 0.001) than the ewes of the same genotype. There was a strong relationship (P 0.001) between MOA and MNA and the fat and lean percentage for 22-month-old ewes. Effects of sire, dam and slaughter day (at the same slaughter age) on MOA and MNA were also found. The results suggest that there is good feasibility for selection against animals at risk of possessing higher levels of short branched-chain fatty acids.

A comparison of solid-phase microextraction (SPME) with simultaneous distillation-extraction (SDE) for the analysis of volatile compounds in heated beef and sheep fats.

Publisher: Elsevier BV

Date: 06-2012

DOI: 10.1016/J.MEATSCI.2011.12.004

Abstract: A comparison has been made on the application of SPME and SDE for the extraction of volatile compounds from heated beef and sheep fats with separation and measurement by gas chromatography-mass spectrometry. As far as we know, this report represents the first time that such a comparison has been made for the measurement of volatile compounds in heated sheep fat. Approximately 100 compounds (in relatively high abundance) were characterised in the volatile profiles of heated beef and sheep fats using both techniques. Differences were observed in the volatile profiles obtained from each technique, independent of compound class. Rather than rate one technique as superior to another, the techniques can be regarded as complementary to each other.

Accuracy of dual energy X-ray absorptiometry, weight, longissimus lumborum muscle depth and GR fat depth to predict half carcass composition in sheep

Publisher: CSIRO Publishing

Date: 2007

DOI: 10.1071/EA07039

Abstract: A Hologic QDR4500W dual energy X-ray absorptiometer (DXA) was used to measure body composition in 60 sheep half carcasses ranging from 8 to 28 kg. Half carcasses were from ewes and wethers of mixed genetics. Values determined by DXA, including total tissue mass (TTM), lean tissue mass (LTM), fat tissue mass (FTM) and bone mineral content (BMC), for the half carcass were evaluated by comparison with chemically determined composition. In the case of BMC, the relationship was with chemically determined ash content. Liveweight and chemically determined lean, fat and ash were strongly related to DXA-derived values for TTM, LTM, FTM and BMC, respectively (R2 = 0.999, 0.986, 0.989 and 0.920, respectively). However, because DXA estimates were different from chemically determined values in this s le of carcasses, they needed to be adjusted with the use of appropriate regression equations to correct the in-built algorithms. These data demonstrate the efficacy of DXA as a non-destructive method for determining the composition of the sheep half carcass.

Conjugated linoleic acid decreases fat accretion in pigs: evaluation by dual-energy X-ray absorptiometry

Publisher: Cambridge University Press (CUP)

Date: 02-2003

DOI: 10.1079/BJN2002765

Abstract: Thirty female Large White × Landrace pigs (average weight 57·2 (SD 1·9) kg) were allocated to one of six dietary treatments containing 0, 1·25, 2·5, 5·0, 7·5 or 10·0 g 55 % conjugated linoleic acids (CLA) isomers (CLA-55)/kg diet and fed for 8 weeks. Each pig was scanned at 0, 28 and 56 d and again at post slaughter using dual-energy X-ray absorptiometry (DXA) to determine the temporal pattern of body composition responses. Values determined by DXA were adjusted using regression equations generated from validation experiments between chemically and DXA-predicted values. Overall, there was a significant linear reduction in fat content with the increasing levels of CLA in the diet ( P =0·007, P =0·011, P =0·008 at week 4, week 8 and for the carcass, respectively). The greatest improvement was recorded at the early stages of CLA supplementation and for the highest dose of CLA (week 4, −19·2 % compared with week 8, −13·7 %). In the first 4 weeks of feeding CLA, pigs receiving 10 g CLA-55/kg diet deposited 93 g less fat/d than pigs fed basal diets ( P =0·002) compared with only 6 g less fat than control animals in the final 4 weeks. Lean content and lean deposition rate were maximised at 5 and 2·5 g CLA-55/kg diet for the first 4 weeks ( P =0·016) and the final 4 weeks of treatment (P=0·17), respectively. DXA estimates of bone mineral content and bone mineral density were not affected by CLA supplementation throughout the experiment. These data demonstrate that dietary CLA decreases body fat in a dose-dependent manner and that the response is greatest over the initial 4 weeks of treatment.

Intestinal Gas Capsules: A Proof-of-Concept Demonstration

Publisher: Elsevier BV

Date: 2016

DOI: 10.1053/J.GASTRO.2015.07.072

Quantitative Kinetoplast DNA Assessment During Treatment of Mucosal Leishmaniasis as a Potential Biomarker of Outcome: A Pilot Study

Publisher: American Society of Tropical Medicine and Hygiene

Date: 06-01-2016

DOI: 10.4269/AJTMH.15-0514

Effects of Raw and Pasteurized Camel Milk on Metabolic Responses in Pigs Fed a High-Fat Diet

Publisher: MDPI AG

Date: 30-06-2022

DOI: 10.3390/ANI12131701

Abstract: Evidence suggests that camel milk (CM) can have insulin-like actions, although the mode of action is not understood. Using the pig as a monogastric model, this pilot experiment examined the effects of CM consumption on metabolic responses to an in vitro glucose tolerance test (IVGTT). Twenty female Large White × Landrace pigs were in idually housed for 6 wks and randomly allocated to one of the following four diets (fed ad libitum n = 5): control (Con) high fat (HF ~16% fat) raw CM (the HF diet plus 500 mL CM/ day) or pasteurized CM (PCM). Blood s les were collected on two occasions (weeks 2 and 5). At week 6, the pigs were fitted with an ear vein cannula and the following day an in vitro glucose tolerance test (IVGTT) was conducted (0.3 g/kg BW glucose). Plasma fatty acids and cholesterol concentrations were greater in the pigs fed the HF diet and greatest in those fed CM, while there was no effect of diet on insulin concentrations. The pigs fed CM tended to have a reduced peak insulin (p = 0.058) and an increased glucose nadir (p = 0.009) in response to the IVGTT. These preliminary results tend to support the hypothesis that feeding CM can improve glycemic control in pigs.

Biology and regulation of carcass composition

Publisher: Elsevier

Date: 2009

DOI: 10.1533/9781845695439.1.19

Dietary lupins (Lupinus angustifolius and Lupinus albus) can increase digesta retention in the gastrointestinal tract of pigs

Publisher: CSIRO Publishing

Date: 2001

DOI: 10.1071/AR00081

Abstract: Lupins are high in crude protein, cell wall materials, and gross energy but uncertainty about the bioavailability of nutrients and adverse effects on feed intake limit their use in the pig industry. Three experiments were conducted to determine the effect of lupins on retention time in the digestive tract by determining the average time it took for ingested polyethylene beads to pass through the digestive tract of pigs fed wheat-based diets containing various lupin species and fractions. In Expt 1, pigs were restrictively fed (1.8 kg/day) diets containing either predominantly wheat or predominantly wheat plus 400 g/kg peas or L. angustifolius seeds or kernels. The retention times of diets containing 400 g/kg of L. angustifolius seeds or kernels were significantly greater than those containing wheat alone (66.4 and 64.3 v. 48.8 h, P 0.05, s.e.d. = 7.7 h), while that for the diet containing 400 g/kg peas was intermediate (55.8 h). In Expt 2 and 3, pigs were fed 1 of 6 wheat-based diets balanced for digestible energy (DE) and amino acid composition and containing either animal protein or 350 g/kg of peas, and L. angustifolius seeds or kernels, or L. albus seeds and kernels. Pigs were restrictively fed (1.5 kg/day) for Expt 2 or fed ad libitum for Expt 3. The mean retention time of the L. albus diets was greater than the other diets in both experiments. Feed intake and daily gain were reduced in pigs fed diets containing L. albus. Despite having lower feed intakes, pigs fed diets containing L. albus had more digesta in the stomach and small intestine at slaughter than the pigs consuming the diets not containing lupins. Appropriate physical treatment or enzymatic supplementation of L. albus diets may alleviate some of the feed intake problems.

Non-esterified fatty acid and glycerol kinetics and fatty acid re-esterification in goats during early lactation

Publisher: Cambridge University Press (CUP)

Date: 07-1990

DOI: 10.1079/BJN19900016

Abstract: Non-esterified fatty acid (NEFA) and glycerol kinetics were studied in lactating goats to gain insight into the mechanisms by which animals in early lactation can initially mobilize and later replenish body fat reserves. Kinetic measurements were made at days 10, 38 and 76 post-partum in ten multiparous lactating does. Plasma NEFA concentrations and NEFA entry rate decreased as lactation advanced, being significantly higher at day 10 than at either days 38 or 76 of lactation. Both plasma NEFA concentrations and NEFA entry rate were negatively correlated with calculated energy balance. In contrast, glycerol entry rate was significantly higher at day 76 than at day 10 of lactation and was positively related to both calculated energy intake and energy balance. Apparent intracellular fatty acid re-esterification was lower at day 10 than at later stages of lactation and was positively related to calculated energy balance. It is suggested that during early lactation, substantial shifts in adipose tissue fat reserves can occur via altering rates of fatty acid re-esterification and de novo lipogenesis, without major changes in the rate of lipolysis.

Body composition changes in goats during early lactation estimated using a two-pool model of tritiated water kinetics

Publisher: Cambridge University Press (CUP)

Date: 07-1990

DOI: 10.1079/BJN19900015

Abstract: A two-pool model of tritiated water kinetics was used to estimate the major body water pools, and hence body composition, in goats at days 10, 38 and 76 of lactation. Between days 10 and 38 of lactation goats were, on average, in negative calculated energy balance and were estimated to have mobilized 59 g body fat stores/d. Mean calculated energy balance over days 38–76 of lactation was slightly positive and there was little change in estimated body fat. Gut fill increased over the early part of lactation when goats were mobilizing body fat. Consequently, live weight did not differ at any stage of lactation and did not provide a good index of body fat status of the goats. There were also no significant differences in empty-body-weight, water, protein, ash or fat-free mass at the three stages of lactation. As average calculated energy balance and changes in energy stored as fat were highly correlated, it is concluded that the two-pool model of tritiated water kinetics is a useful means of serially estimating changes in body fat content in unfasted lactating goats.

Dietary onion intake as part of a typical high fat diet improves indices of cardiovascular health using the mixed sex pig model

Publisher: Springer Science and Business Media LLC

Date: 22-11-2006

DOI: 10.1007/S11130-006-0030-8

Abstract: The aim of this study was to evaluate the potential health benefits of onions consumed at two levels of intake, using the pig model. The dietary fat content was set at a level typical of a "western" diet (25% w/w). Fifteen female and fifteen male pigs (Large White x Landrace) were allocated to one of three dietary treatments in a randomised block design. Treatments consisted of control diet (no onion) and onion supplementation at either 8.6 or 21.4 g of onion/MJ DE fed for six weeks. Onion consumption reduced plasma triglyceride levels by 15% (P=0.030) regardless of sex and onion dose. Total plasma cholesterol and cholesterol fractions were unaffected by onion supplementation (P>0.050). The bioactivity of onion was evident in haematocrit measures, where red blood cell and haemoglobin were significantly reduced in a dose dependant manner (P<0.001 and P=0.011, respectively), while other cell counts, with exception of segmented neutrophils (-18%, P=0.012), were largely unaffected. Serum oxidative status was improved (P=0.007) in pigs consuming onions. These data demonstrate that consumption of onions can have positive health effects in both male and female pigs consuming a high fat diet.

Effect of processing on bioaccessibility and bioavailability of bioactive compounds in coffee beans

Publisher: Elsevier BV

Date: 04-2022

DOI: 10.1016/J.FBIO.2021.101373

Association of Phenotypic Markers of Heat Tolerance with Australian Genomic Estimated Breeding Values and Dairy Cattle Selection Indices

Publisher: MDPI AG

Date: 10-07-2023

DOI: 10.3390/ANI13142259

Abstract: Dairy cattle predicted by genomic breeding values to be heat tolerant are known to have less milk production decline and lower core body temperature increases in response to elevated temperatures. In a study conducted at the University of Melbourne’s Dookie Robotic Dairy Farm during summer, we identified the 20 most heat-susceptible and heat-tolerant cows in a herd of 150 Holstein Friesian lactating cows based on their phenotypic responses (changes in respiration rate, surface body temperature, panting score, and milk production). Hair s les were collected from the tip of the cows’ tails following standard genotyping protocols. The results indicated variation in feed saved and HT genomic estimated breeding values (GEBVs) (p ≤ 0.05) across age, indicating a potential for their selection. As expected, the thermotolerant group had higher GEBVs for HT and feed saved but lower values for milk production. In general, younger cows had superior GEBVs for the Balanced Performance Index (BPI) and Australian Selection Index (ASI), whilst older cows were superior in fertility, feed saved (FS), and HT. This study demonstrated highly significant (p ≤ 0.001) negative correlations (−0.28 to −0.74) between HT and GEBVs for current Australian dairy cattle selection indices (BPI, ASI, HWI) and significant (p ≤ 0.05) positive correlations between HT and GEBVs for traits like FS (0.45) and fertility (0.25). Genomic selection for HT will help improve cow efficiency and sustainability of dairy production under hot summer conditions. However, a more extensive study involving more lactating cows across multiple farms is recommended to confirm the associations between the phenotypic predictors of HT and GEBVs.

Relations between plasma non-esterified fatty acid metabolism and body tissue mobilization during chronic undernutrition in goats

Publisher: Cambridge University Press (CUP)

Date: 11-1988

DOI: 10.1079/BJN19880133

Abstract: 1. Eleven mature goats were offered 140 kJ metabolizable energy/kg per d (M) of lucerne ( Medicago sativa ) hay-oaten grain (1:1, w/w) for at least 1 month before plasma non-esterified fatty acid (NEFA) kinetics and tritiated water space (TS) were determined. 2. Goats were then fed at M, 0.5 M or 0.25 M for 34 ( se 6) d, at which time the experimental procedures were repeated. 3. Chronic undernutrition resulted in elevated NEFA concentrations and NEFA entry rate, with a tendency for the ratio plasma NEFA:glycerol to increase, suggesting that body-fat mobilization during prolonged underfeeding is due more to decreased lipogenesis and intracellular NEFA re-esterification rather than to increased lipolysis. 4. Plasma NEFA concentrations and NEFA entry rate, as well as being highly correlated with each other, were significantly related to calculated energy balance and body fat losses estimated from changes in live weight and TS. 5. Increases in NEFA entry rate were highly correlated with, and of the same magnitude as, body fat losses, confirming that NEFA kinetics do quantitatively reflect lipid mobilization.

Bioaccessibility of phenolic compounds from sesame seeds ( Sesamum indicum L.) during in vitro gastrointestinal digestion and colonic fermentation

Publisher: Hindawi Limited

Date: 26-04-2022

DOI: 10.1111/JFPP.16669

Characterization, Antioxidant Potential, and Pharmacokinetics Properties of Phenolic Compounds from Native Australian Herbs and Fruits

Publisher: MDPI AG

Date: 21-02-2023

DOI: 10.3390/PLANTS12050993

Abstract: In recent decades, plant bioactive phenolic compounds gained much attention due to their various health benefits. Therefore, this study aimed to analyze native Australian river mint (Mentha australis), bush mint (Mentha satureioides), sea parsley (Apium prostratum), and bush tomatoes (Solanum centrale) for their bioactive metabolites, antioxidant potential, and pharmacokinetics properties. LC-ESI-QTOF-MS/MS was applied to elucidate these plants’ composition, identification, and quantification of phenolic metabolites. This study tentatively identified 123 phenolic compounds (thirty-five phenolic acids, sixty-seven flavonoids, seven lignans, three stilbenes, and eleven other compounds). Bush mint was identified with the highest total phenolic content (TPC—57.70 ± 4.57 mg GAE/g), while sea parsley contained the lowest total phenolic content (13.44 ± 0.39 mg GAE/g). Moreover, bush mint was also identified with the highest antioxidant potential compared to other herbs. Thirty-seven phenolic metabolites were semi-quantified, including rosmarinic acid, chlorogenic acid, sagerinic acid, quinic acid, and caffeic acid, which were abundant in these selected plants. The most abundant compounds’ pharmacokinetics properties were also predicted. This study will develop further research to identify these plants’ nutraceutical and phytopharmaceutical potential.

Moderate doses of porcine somatotropin do not increase plasma insulin- like growth factor-I (IGF-I) or IGF binding protein-3

Publisher: Elsevier BV

Date: 04-1999

DOI: 10.1016/S0739-7240(99)00010-7

Abstract: The growth rate of the young pig is generally much less than its potential and may be constrained by endocrine status as well as by nutrient intake. The aim of this study was to determine whether porcine somatotropin (pST) could increase growth in the nursing pig. Fourteen sows nursing litters of 6 (n = 7) or 12 (n = 7) piglets were utilized to establish a high and low plane of nutrition for sucking pigs. On Day 4 of lactation, the median two male pigs from each litter were randomly allocated to one of two doses of pST (0 or 60 micrograms/kg/d) until weaning on Day 31. Pigs were bled on Days 4, 13, 22, and 31 of lactation and the plasma was analyzed for insulin-like growth factor (IGF)-I, IGF-II, and IGF binding protein-3 (IGFBP-3). Pigs were weaned into conventional accommodation and further weighed on Days 63, 91, and 119. Pigs from litters of 6 grew more quickly and weighed 2.2 kg (P = 0.01) and 3.5 kg (P = 0.04) more than pigs from litters of 12 at 31 and 63 d of age, respectively. There was no effect of pST on preweaning growth of sucking pigs (261 vs. 258 g/d, P = 0.68), although growth rate increased in the final 3 d before weaning at 31 d (241 vs. 294 g/d, P = 0.01). IGFBP-3 was greater (1.09 vs. 0.78 micrograms/ml, P < 0.001), whereas IGF-I tended to be greater (206 vs. 176 ng/ml, P = 0.14), in pigs from the small litters. There was no effect of pST on plasma IGF-I (182 vs. 195 ng/ml, P = 0.454) or IGFBP-3 (0.93 vs. 0.94 microgram/ml, P = 0.85) concentrations. Plasma IGF-I and IGFBP-3 were highly correlated with the growth rate of nursing pigs (R = 0.638 and 0.756, respectively). There were no effects of pST (340 vs. 328 ng/ml, P = 0.48) or litter size (336 vs. 333 ng/ml, P = 0.88) on IGF-II. In conclusion, pST had no little or no effect on growth performance or plasma IGF-I, IGF-II, or IGFBP-3 in sucking pigs on either a high or low plane of nutrition.

Effects of dietary factors and other metabolic modifiers on quality and nutritional value of meat

Publisher: Elsevier BV

Date: 09-2005

DOI: 10.1016/J.MEATSCI.2005.05.001

Abstract: A number of technologies that increase feed efficiency and lean tissue deposition while decreasing fat deposition have been developed in an effort to improve profitability of animal production. In general, the mode of action of these metabolic modifiers is to increase muscle deposition while often simultaneously reducing fat deposition. However, there have been some concerns that the focus on increasing production efficiency and lean meat yield has been to the detriment of meat quality. The aim of this review is to collate data on the effects of these metabolic modifiers on meat quality, and then discuss these overall effects. When data from the literature are collated and subject to meta-analyses it appears that conservative use of each of these technologies will result in a 5-10% (0.3-0.5kg) increase in shear force with a similar reduction in perception of tenderness. However, it should be borne in mind that the magnitude of these increases are similar to those observed with similar increases in carcass leanness obtained through other means (e.g. nutritional, genetic selection) and may be an inherent consequence of the production of leaner meat. To counter this, there are some other metabolic factors and dietary additives that offer some potential to improve meat quality (for ex le immuncastration) and it is possible that these can be used on their own or in conjunction with somatotropin, approved β-agonists, anabolic implants and CLA to maintain or improve meat quality.

Nano Chromium Picolinate Improves Gene Expression Associated with Insulin Signaling in Porcine Skeletal Muscle and Adipose Tissue

Publisher: MDPI AG

Date: 18-09-2020

DOI: 10.3390/ANI10091685

Abstract: The aim of this study was to investigate the interactive effects of dietary nano chromium picolinate (nCrPic) and dietary fat on genes involved in insulin signaling in skeletal muscle and subcutaneous adipose tissue of pigs. Forty-eight gilts were stratified on body weight into four blocks of four pens of three pigs and then within each block each pen was randomly allocated to four treatment groups in a 2 × 2 factorial design. The respective factors were dietary fat (22 or 57 g/kg) and dietary nCrPic (0 or 400 ppb nCrPic) fed for six weeks. Skeletal muscle s les were collected from the Longissimus thoracis and subcutaneous adipose tissue collected from above this muscle. Dietary nCrPic increased adiponectin, uncoupling protein 3 (UCP3) and serine/threonine protein kinase (AKT) mRNA expression, whereas dietary fat decreased adiponectin and increased leptin, tumor necrosis factor-α (TNF-α), peroxisome proliferator-activated receptors γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) mRNA expression in adipose tissue. In skeletal muscle, dietary nCrPic increased phosphatidylinositol 3 kinase (PI3K), AKT, UCP3 and interleukin-15 (IL-15), as well as decreased suppressor of cytokine signaling 3 (SOCS3) mRNA expression. The improvement in insulin signaling and muscle mass and the reduction in carcass fatness by dietary nCrPic may be via decreased SOCS3 and increased UCP3 and IL-15 in skeletal muscle and increased adiponectin in subcutaneous adipose tissue.

Dietary nano chromium picolinate can ameliorate some of the impacts of heat stress in cross-bred sheep

Publisher: Elsevier BV

Date: 03-2021

DOI: 10.1016/J.ANINU.2020.07.004

Physicochemical properties of dietary phytochemicals can predict their passive absorption in the human small intestine

Publisher: Springer Science and Business Media LLC

Date: 16-05-2017

DOI: 10.1038/S41598-017-01888-W

Abstract: A diet high in phytochemical-rich plant foods is associated with reducing the risk of chronic diseases such as cardiovascular and neurodegenerative diseases, obesity, diabetes and cancer. Oxidative stress and inflammation (OSI) is the common component underlying these chronic diseases. Whilst the positive health effects of phytochemicals and their metabolites have been demonstrated to regulate OSI, the timing and absorption for best effect is not well understood. We developed a model to predict the time to achieve maximal plasma concentration (T max ) of phytochemicals in fruits and vegetables. We used a training dataset containing 67 dietary phytochemicals from 31 clinical studies to develop the model and validated the model using three independent datasets comprising a total of 108 dietary phytochemicals and 98 pharmaceutical compounds. The developed model based on dietary intake forms and the physicochemical properties lipophilicity and molecular mass accurately predicts T max of dietary phytochemicals and pharmaceutical compounds over a broad range of chemical classes. This is the first direct model to predict T max of dietary phytochemicals in the human body. The model informs the clinical dosing frequency for optimising uptake and sustained presence of dietary phytochemicals in circulation, to maximise their bio-efficacy for positively affect human health and managing OSI in chronic diseases.

Reduced protein intake during the weaner period has variable effects on subsequent growth and carcass composition of pigs

Publisher: CSIRO Publishing

Date: 2007

DOI: 10.1071/EA06059

Abstract: In total, 960 pigs (480 castrated males and 480 gilts), selected at ~28 days of age, were used to investigate the effect of restricting protein intake for a short period between 4 and 14 weeks of age. Pigs were selected over an 8-week period in groups of 120 pigs (three pens of 20 castrated males and three pens of 20 gilts per week). Pigs were given ad libitum access to commercial diets from weaning to slaughter. Pens of 20 pigs of each sex were allocated to one of six treatments: control (no restriction), restriction from 4 to 7 weeks of age, restriction from 7 to 10 weeks of age, restriction from 10 to 14 weeks of age, restriction from 4 to 10 weeks of age or a restriction from 4 to 14 weeks of age. During the period of restriction, pigs were offered ad libitum commercial diets that had been reduced in dietary lysine to digestible energy ratio by ~15%. Restricting protein intake between 7 and 10, 4 and 10 and 4 and 14 weeks of age reduced daily gain during the period of restriction. Pigs restricted from 7 to 10 weeks of age had reduced average daily feed intake (5.6%) from 7 to 10 weeks, followed by a reduction in average daily feed intake (5.5%) and improved feed efficiency (6.1%) in the subsequent period from 10 to 17 weeks of age. Daily gain for the entire experimental period was similar for all treatment groups, except those restricted from 7 to 10 weeks of age (3.9% lower than the controls), although there was no treatment effect on carcass weight. Interestingly, backfat was reduced by 10% in these animals compared with the controls, which may be economically beneficial in markets where producers are paid on backfat and carcass weight, such as those selling domestically in the Australian market. Economic benefits of reduced feed costs during both the restriction and realimentation periods were also observed when pigs were restricted from 10 to 14 weeks of age. Any economic benefits from short periods of protein restriction will vary depending on the specific market conditions and the local cost of feed protein sources.

Primiparous and Multiparous Sows Have Largely Similar Colostrum and Milk Composition Profiles Throughout Lactation

Publisher: MDPI AG

Date: 26-01-2019

DOI: 10.3390/ANI9020035

Abstract: It is important to understand the biological factors influencing the poorer lifetime performance of gilt progeny in comparison to sow progeny and determine whether this may be partially due to differences in lactation performance between primiparous and multiparous sows. It was hypothesized that primiparous sows would have lower levels of immunoglobulin G (IgG) in colostrum and milk compared to multiparous sows, and lower levels of other energetic components. Differences in colostrum and milk composition between ten primiparous and ten multiparous sows (parities 3 and 4) from a commercial herd were examined throughout lactation (day 0, 1, 2, 3, 7, 14, and 21). Overall, there were no (p ≥ 0.05) parity differences in total IgG, fat, protein, lactose, and net energy (NE) concentrations. Primiparous sows had higher lactose levels at day 2 (parity by timepoint interaction p = 0.036) and lower NE at day 3 (p = 0.091), and multiparous sows had higher lactose levels at days 14 and 21. Results suggest that shortcomings of gilt progeny are unlikely due to insufficient nutrient levels in colostrum and milk, and more likely to reduced colostrum and milk intake and their capacity to digest and absorb each component.

Exogenous porcine somatotropin administered to neonatal pigs at high doses can alter lifetime fat but not lean tissue deposition

Publisher: Cambridge University Press (CUP)

Date: 06-2003

DOI: 10.1079/BJN2003843

Abstract: The growth rate of the young pig is generally much less than its potential and may be constrained by endocrine status as well as nutrient intake. The aim of the present study was to determine whether porcine (p) somatotropin (ST) treatment of the sucking pig could alter subsequent body composition. Twelve mixed-parity cross-bred sows with an average litter size of ten piglets were used to nurse pigs for the present study. On day 1 of lactation, the median two male pigs (by weight) from each litter were randomly allocated to one of two doses of pST (0 or 1 mg/kg per d) until weaning on day 21. Pigs were weaned and offered feed ad libitum until slaughter at 134 d of age. Body composition was measured using dual-energy X-ray absorptiometry (DXA) at 21, 49, 77, 105 and 133 d of age. There was no significant difference in growth rates between day 1 and 21 of lactation in pigs injected with either saline (9 g/l NaCl/l) or pST (258 v. 246 g/d for control and pST-treated pigs respectively, P =0·61), and as a consequence there was no significant difference in liveweight at weaning (7·13 v. 6·84 kg, P =0·59). However, fat mass at weaning tended to be decreased (1·18 v. 0·96 kg, P =0·064), while the % fat in the body at weaning was significantly (16·7 v. 13·9 %, P =0·008) decreased by exogenous pST treatment. In the immediate post-weaning period there was a reduction in lean tissue deposition (347 v. 300 g/d, P =0·021) but no effect on fat deposition (35 v. 33 g/d, P =0·72). Over the entire weaning-to-slaughter period, pST treatment of neonatal pigs decreased the rate of fat deposition (130 v. 112 g/d, P =0·033), but had no effect on lean tissue deposition (550 v. 538 g/d, P =0·49). Therefore, treatment of nursing pigs with high doses of pST for a short period before weaning may provide a means of reducing the fat content of pork and pork products.

Variation in feeding behavior and milk production among dairy cows when supplemented with 2 amounts of mixed ration in combination with 2 amounts of pasture

Publisher: American Dairy Science Association

Date: 08-2016

DOI: 10.3168/JDS.2015-10771

Abstract: Variation in feeding behavior and milk production of grazing dairy cows fed a mixed ration was measured. Experiments were conducted in spring (early lactation) and autumn (late lactation) with 48 Holstein-Friesian dairy cows. Pasture allowance (low vs. high) and amounts of supplement (low vs. high) were applied to determine the effect on variation among cows in feeding behavior and milk production. The experiments investigated 4 dietary treatments in a 2×2 factorial arrangement of treatments. Daily pasture allowances were 15kg of DM/cow per day (low) and 37kg of DM/cow per day (high to ground level) and 12kg of DM/cow per day (low) and 31kg of DM/cow per day (high to ground level), for the spring and autumn experiments, respectively. Supplements were offered at 6kg of DM/cow per day (low) and 14kg of DM/cow per day (high) and 6kg of DM/cow per day (low) and 12kg of DM/cow per day (high), for the spring and autumn experiments, respectively. There were 2groups of 6 cows per treatment. All treatments received a partial mixed ration, defined as a total mixed ration fed between periods of grazing that contained wheat grain, corn grain, alfalfa hay, and canola meal. The grain-to-forage ratio of the supplements was 78:22 (DM basis) in both spring and autumn. In both experiments, the pre-experimental period was 14d followed by a 10-d experimental period. The variation among cows within a group in feeding behavior was influenced by the amount of supplement but not the amount of pasture offered. The variation among cows in pasture eating time approximately doubled when the amount of supplement offered increased, indicating that to reduce the variability among cows, supplement feeding management strategies need to be considered. Increasing pasture allowance had no effect on pasture eating time although pasture intake increased as a result of increased grazing intensity compared with the low pasture allowance. However, increasing the amount of supplement in the partial mixed ration feeding system reduced pasture eating time by 51min/cow per day.

Maternal heat stress alters expression of genes associated with nutrient transport activity and metabolism in female placentae from mid‐gestating pigs

Publisher: MDPI AG

Date: 16-04-2021

DOI: 10.3390/IJMS22084147

Abstract: Placental insufficiency is a known consequence of maternal heat stress during gestation in farm animals. The molecular regulation of placentae during the stress response is little known in pigs. This study aims to identify differential gene expression in pig placentae caused by maternal heat exposure during early to mid-gestation. RNA sequencing (RNA-seq) was performed on female placental s les from pregnant pigs exposed to thermoneutral control (CON constant 20 °C n = 5) or cyclic heat stress (HS cyclic 28 to 33 °C n = 5) conditions between d40 and d60 of gestation. On d60 of gestation, placental efficiency (fetal lacental weight) was decreased (p = 0.023) by maternal HS. A total of 169 genes were differentially expressed (FDR ≤ 0.1) between CON and HS placentae of female fetuses, of which 35 genes were upregulated and 134 genes were downregulated by maternal HS. The current data revealed transport activity (FDR = 0.027), glycoprotein biosynthetic process (FDR = 0.044), and carbohydrate metabolic process (FDR = 0.049) among the terms enriched by the downregulated genes (HS vs. CON). In addition, solute carrier (SLC)-mediated transmembrane transport (FDR = 0.008) and glycosaminoglycan biosynthesis (FDR = 0.027), which modulates placental stroma synthesis, were identified among the pathways enriched by the downregulated genes. These findings provide evidence that heat-stress induced placental inefficiency may be underpinned by altered expression of genes associated with placental nutrient transport capacity and metabolism. A further understanding of the molecular mechanism contributes to the identification of placental gene signatures of summer infertility in pigs.

The Quest for Phenolic Compounds from Seaweed: Nutrition, Biological Activities and Applications

Publisher: Informa UK Limited

Date: 03-07-2023

DOI: 10.1080/87559129.2022.2094406

Starch but not protein digestibility is altered in pigs fed transgenic peas containing α-amylase inhibitor

Publisher: Wiley

Date: 12-06-2006

DOI: 10.1002/JSFA.2551

Chemical characterisation and speciation of organic selenium in cultivated selenium-enriched Agaricus bisporus

Publisher: Elsevier BV

Date: 12-2013

DOI: 10.1016/J.FOODCHEM.2013.06.027

Abstract: The selenium concentration in Agaricus bisporus cultivated in growth compost irrigated with sodium selenite solution increased by 28- and 43-fold compared to the control mushroom irrigated solely with water. Selenium contents of mushroom proteins increased from 13.8 to 60.1 and 14.1 to 137 μgSe/g in caps and stalks from control and selenised mushrooms, respectively. Selenocystine (SeCys detected as [SeCys]2 dimer), selenomethionine (SeMet), and methyl-selenocysteine (MeSeCys) were separated, identified and quantified by liquid chromatography-electrospray ionisation-mass spectrometry from water solubilised and acetone precipitated proteins, and significant increases were observed for the selenised mushrooms. The maximum selenoamino acids concentration in caps and stalks of control/selenised mushrooms was 4.16/9.65 μg/g dried weight (DW) for SeCys, 0.08/0.58 μg/g DW for SeMet, and 0.031/0.10 μg/g DW for MeSeCys, respectively. The most notable result was the much higher levels of SeCys accumulated by A. bisporus compared to SeMet and MeSeCys, for both control and selenised A. bisporus.

Thermoregulatory differences in lactating dairy cattle classed as efficient or inefficient based on residual feed intake

Publisher: CSIRO Publishing

Date: 2014

DOI: 10.1071/AN14311

Abstract: It is suggested that one-third of the inter-animal differences in efficiency is explained by differences in digestion, heat production, body composition and activity while the remaining variation is the result of energy expenditure due to biological processes such as ion pumps and mitochondrial function. Inefficient animals may be wasting energy on inefficient processes resulting in increased heat production that may be reflected by differences in skin and core temperature. While the association between heat production and residual feed intake (RFI) has been touched on, it is yet to be fully elucidated. It is hypothesised that more efficient animals will expend less energy as heat, which will be reflected by differences in core and skin temperature measures. Fifty-four primiparous, Holstein-Friesian cows previously assessed for RFI (26 inefficient/high RFI, 28 efficient/low RFI) were selected and drafted into outdoor holding yards for measurements on two occasions (once during lactation and once during the non-lactating ‘dry’ period). Measures of body temperature were obtained using an infrared (IR) camera to obtain skin (surface) temperatures at multiple locations [muzzle, eye, jaw, ear, leg (front and back), rump, shoulder, teat, udder, side and tail] and rectal temperatures were measured using a digital thermometer. Respiration rates (RR) were obtained by counting the number of flank movements in 1 min. A subset of 16 cows (8 efficient and 8 inefficient) were utilised for further IR imagery in an undercover environment (to eliminate the influences of external environments). Skin temperature measurement obtained using an IR camera during the outdoor period demonstrated that inefficient cows had higher (0.65°C) teat temperatures (P = 0.05). Rectal temperature and RR were not influenced by efficiency group. When IR images were obtained undercover inefficient cows tended to have higher shoulder (0.85°C) and neck (0.98°C) temperatures than efficient cows (P 0.087) while udder temperature was significantly greater (1.61°C) for inefficient than efficient cows (P = 0.018). These data indicate that some of the differences in efficiency may be attributed to differences in thermoregulation, as reflected by differences in skin (but not core) temperature and that IR imagery is a suitable method for determining these differences in a non-invasive manner. Further research is required to further establish these relationships, and the measurement of skin temperatures should be undertaken indoors to eliminate external environmental influences.

Igf-I variants which bind poorly to IGF-binding proteins show more potent and prolonged hypoglycaemic action than native IGF-I in pigs and marmoset monkeys

Publisher: Bioscientifica

Date: 11-1997

DOI: 10.1677/JOE.0.1550377

Abstract: The relative acute hypoglycaemic potencies of IGF-I and several variants of IGF-I which bind poorly to the IGF-I binding proteins (IGFBPs) have been examined in marmosets (Callithrix jacchus) and the pig. In the marmoset study, IGF-I and des(1-3)IGF-I were compared in anaesthetised and conscious animals in a range of bolus doses from 42 to 270 micrograms/kg body weight. In the pig study, IGF-I was compared with four variants, des(1-3)IGF-I long-IGF-I, R3IGF-I and long-R3IGF-I (LR3IGF-I), which show reduced affinity for the IGFBPs as well as with insulin. Doses in the pig were 20 and 50 micrograms/kg body weight for the IGFs and 3 micrograms/kg for insulin. In each study serial blood s les were taken from 30 min before to 4 h after the bolus injection. Plasma glucose levels were decreased in a dose-responsive manner with the pig more sensitive than either the conscious or anaesthetised marmoset (maximum lowering 4.8, 3.7 and 2.5 mmol/l respectively). The IGF variants were consistently 2- to 3-fold more potent than IGF-I in each animal for lowering of plasma glucose to the nadir, with the potency reflecting the relative affinities for binding to the IGFBPs and the IGF-I receptors. Thus, hypoglycaemic potency was in the order IGF-I long-IGF-I R3IGF-I approximately LR3IGF-I des (1-3)IGF-I. Notably the variants suppressed plasma glucose levels over a much longer period than did IGF-I, the cumulative suppression over four hours showing an approximately 4- to 8-fold increase in the extent of hypoglycaemia. The prolonged suppression was not simply proportional to the hypoglycaemic nadir at doses equipotent for glucose lowering, the cumulative hypoglycaemic effect for the variants in either species was about 2-fold that for IGF-I. The differential effect of the variants in the marmoset could not be accounted for by correlated changes in plasma insulin, IGF-I or IGFBP levels in plasma. Indirect effects via inhibition of glucagon, or direct effects via hepatic insulin receptors are postulated to account for the results. There was a dose-related reduction in plasma amino acids in the pig but, unlike the case for plasma glucose, only one analogue, LR3IGF-I was more potent than IGF-I. The response to LR3IGF-I was accentuated at the high dosage but on the basis of the other variants tested this effect could not be ascribed to either of the incorporated molecular variations. Despite their more rapid clearance from the circulation, variants of IGF-I which show lower affinity for binding to IGFBPs show proportionately superior potency for sustained hypoglycaemic action. Since our data were obtained in animal models of accepted relevance to humans these results point to the possible superior efficacy of the variants, especially des(1-3)IGF-I, over IGF-I for use as an adjunct to insulin treatment of hyperglycaemic conditions.

Bioaccessibility and Bioavailability of Phenolic Compounds in Seaweed

Publisher: Informa UK Limited

Date: 13-07-2023

DOI: 10.1080/87559129.2022.2094404

Dietary chromium picolinate of varying particle size improves carcass characteristics and insulin sensitivity in finishing pigs fed low- and high-fat diets

Publisher: CSIRO Publishing

Date: 2015

DOI: 10.1071/AN12255

Abstract: The aim of the present study was to evaluate the effect of the different-sized particles of chromium (Cr) picolinate (CrPic) and dietary fat on growth performance, carcass traits and insulin sensitivity of finishing pigs. Ninety-six Large White × Landrace gilts were stratified on bodyweight, housed in pens of three pigs and then, on a pen basis, randomly allocated to eight treatment groups in a 2 × 4 factorial design for 6 weeks. The respective factors were dietary fat (2.2% or 5.7%) and dietary CrPic (0 mg/kg, 400 mg/kgnormal-size CrPic, 400 mg/kg 1-μm CrPic (μCrPic), or 400 mg/kg 100-nm CrPic (nCrPic)). Over the first 21 days, average daily gain (ADG) was increased by dietary CrPic (0.94 vs 1.01 kg/day, P = 0.021), although there was no difference (P = 0.17) between the CrPic particle sizes. High dietary fat also increased ADG over this period (0.96 vs 1.03 kg/day, P = 0.013). However, the responses to both dietary CrPic and fat diminished over time and so there was no effect of CrPic (P = 0.35) or fat (P = 0.93) on ADG over the full 42 days. Dietary CrPic increased carcass weight and muscle depth and decreased P2 backfat. Furthermore, dietary Cr decreased plasma insulin (7.66 vs 5.09 mU/L, P = 0.018) and the homeostatic model assessment (1.25 vs 0.82, P = 0.009), indicating an improvement in insulin sensitivity. In conclusion, dietary Cr can increase ADG and improve carcass traits and insulin sensitivity in lean pigs. There were few differences among particle sizes, possibly because the response to dietary CrPic may already be maximised at 400 mg/kg.

Dietary Inclusion of 1,3-Butanediol Increases Dam Circulating Ketones and Increases Progeny Birth Weight

Publisher: MDPI AG

Date: 24-07-2019

DOI: 10.3390/ANI9080479

Abstract: 1,3-Butanediol (BD) is a ketogenic substance that can improve piglet growth and survival and potentially increase performance in gilt progeny when provided as a dietary supplement during late gestation. Gilts (n = 77 parity 1) and sows (n = 74 parities 2 and 3) were fed either a standard commercial gestation diet or a diet supplemented with 4% BD from day 90 of gestation until farrowing. Dams fed with diets supplemented with BD had higher plasma beta-hydroxybutyrate (p = 0.01) and lower non-esterified fatty acid concentrations (p 0.001). The percentage of progeny that were light-for-age ( .1 kg) at birth was decreased by BD (18.2 vs. 13.5%, p 0.006), particularly in gilts (24.0 vs. 18.3%, p 0.034). In idual birth weights and litter weights birth weights tended to be increased by the BD diet (p = 0.085 and 0.078 respectively) although these effects were not maintained to weaning. Pre-weaning mortality was greater in gilt than in sow progeny and was not altered by dietary BD. Feeding BD in late gestation can improve birth weight, but further work is needed to see if these effects are carried through subsequent stages of growth, particularly in gilt progeny.

Polyunsaturated fats in meat from Merino, first- and second-cross sheep slaughtered as yearlings

Publisher: Elsevier BV

Date: 10-2009

DOI: 10.1016/J.MEATSCI.2009.05.018

Abstract: This study examined the level of long chain omega-3 and omega-6 polyunsaturated fats, the ratio of polyunsaturated fat to saturated fat (PUFA/SFA) and the ratio of omega-6 to omega-3 (n-6/n-3) fat in sheep grown under grazing conditions in Australia. The sheep genotypes used were Poll Dorset(growth)×Border Leicester Merino (PDg×BLM), Poll Dorset(growth)×Merino (PDg×M), Poll Dorset(muscling)×Merino (PDm×M), Border Leicester×Merino (BL×M) and Merino×Merino (M×M). Loin muscles (Longissimus lumborum) collected from 40 ewe and wether sheep slaughtered at 14months of age were processed for fatty acid determination. After frozen storage, 20g s les were minced and a 7g homogenate was processed for muscle lipid extraction using a chloroform:methanol (2:1) procedure. There was an increase in PUFA/SFA as the proportion of Merino genetics increased in the progeny (second-cross<first-cross<Merino), but this was not shown in the n-6/n-3 ratio. The PUFA/SFA trend appeared to be associated with an increase in the level of total polyunsaturated fats, but not a decrease in the level of total saturated fats. The results demonstrate that there is a need to improve the PUFA/SFA content in first- and second-cross animals which are mainly used for meat production in Australia so as to maintain the healthy lipids in meat. Nutritional manipulation through feeding systems or selection of sires for greater heritability of omega-3 fat deposition may be suitable pathways to elevate the ratio of polyunsaturated fatty acids, and in particular omega-3.

The Effect of Soundwaves on Foamability Properties and Sensory of Beers with a Machine Learning Modeling Approach

Publisher: MDPI AG

Date: 26-07-2018

DOI: 10.3390/BEVERAGES4030053

Abstract: The use of ultrasounds has been implemented to increase yeast viability, de-foaming, and cavitation in foods and beverages. However, the application of low frequency audible sound to decrease bubble size and improve foamability has not been explored. In this study, three treatments using India Pale Ale beers were tested, which include (1) a control, (2) the application of audible sound during fermentation, and (3) the application of audible sound during natural carbonation. Five different audible frequencies (20 Hz, 30 Hz, 45 Hz, 55 Hz, and 75 Hz) were applied daily for one minute each (starting from the lowest frequency) during fermentation (11 days, treatment 2) and carbonation (22 days, treatment 3). S les were measured in triplicates using the RoboBEER to assess color and foam-related parameters. A trained panel (n = 10) evaluated the intensity of sensory descriptors. Results showed that s les with sonication treatment had significant differences in the number of small bubbles, alcohol, and viscosity compared to the control. Furthermore, except for foam texture, foam height, and viscosity, there were non-significant differences in the intensity of any sensory descriptor, according to the rating from the trained sensory panel. The use of soundwaves is a potential treatment for brewing to improve beer quality by increasing the number of small bubbles and foamability without disrupting yeast or modifying the aroma and flavor profile.

Ractopamine hydrochloride improves growth performance and carcass composition in immunocastrated boars, intact boars, and gilts

Publisher: Oxford University Press (OUP)

Date: 11-2009

DOI: 10.2527/JAS.2009-2002

Abstract: The beta-agonist ractopamine is a dietary ingredient that improves growth and increases the lean mass with little change in fat mass in gilts and barrows. Limited data in boars indicate that dietary ractopamine may increase lean tissue and decrease fat deposition, whereas there are no data for immunocastrated boars. The aims of this investigation were 1) to assess whether the growth performance of all sexes could be maintained over 31 d by using a step-up dietary ractopamine feeding program of 5 mg/kg of ractopamine for the first 14 d, then increasing the dose to 10 mg/kg for a further 17 d, and 2) to determine if dietary ractopamine would increase lean mass in all sexes and decrease fat mass in boars and immunocastrated boars. The study involved 286 pigs randomized and proportionally allocated by breed into 24 groups of 11 or 12 pigs at 17 wk of age, with equal groups of boars, immunocastrated boars, and gilts. Dietary ractopamine decreased (P = 0.005) ADFI during the first 2 wk, particularly in the intact and immunocastrated boars, with the reduction in ADFI being maintained in the immunocastrated boars after the increment in dietary ractopamine. Daily BW gain was not altered by dietary ractopamine during the first 2 wk, but was increased (P < 0.001) after the increment in dietary ractopamine. Dietary ractopamine decreased (P < or = 0.033) feed conversion ratio in all sexes with the response being greater after the increase in dietary ractopamine. Carcass weight was increased (P < 0.001) by dietary ractopamine in all sexes, whereas back fat tended (P = 0.076) to be reduced in the immunocastrated boars. Dietary ractopamine increased (P = 0.018) lean tissue mass by 4.0, 4.8, and 6.5 kg in the intact boars, gilts, and immunocastrated boars, respectively. In the entire and immunocastrated boars, the increase in lean tissue was accompanied with a decrease (P = 0.004) in fat mass. There was little effect of dietary ractopamine on fat mass in gilts. However, carcass percent fat was decreased (P = 0.004) and percent lean increased (P = 0.006) in all sexes. Immunocastration caused a decrease in lean tissue mass and an increase in fat mass and an increase in ADFI in the last one-half of the study. Dietary ractopamine may decrease fat mass in intact and immunocastrated boars and offers an excellent means of maximizing the effects of immunocastration and minimizing the increase in fat mass sometimes observed in immunocastrated boars.

Interactions between piglet weaning age and dietary creep feed composition on lifetime growth performance

Publisher: CSIRO Publishing

Date: 2013

DOI: 10.1071/AN12009

Abstract: The influences of creep feed composition and piglet weaning age on apparent creep feed disappearance and post-weaning performance were examined. A total of 24 gilts and 72 multiparous sows were selected at farrowing, and their litters allocated to a 2 by 2 factorial arrangement of treatments with the factors being weaning age (22 or 29 days of age) and the composition of the creep feed [simple (diet based predominately on cereals and animal and vegetable protein sources) or complex (diet based predominately on cereals, animal protein sources and 300 g/kg whey powder)]. Creep feed was offered to all litters from 9 days of age to weaning. While creep feed disappearance did not affect pre-weaning growth performance, there was a greater disappearance of the simple creep feed than the complex creep feed from 9 days of age to weaning (776 versus 461 g/litter, respectively, P = 0.004). Apparent creep feed intake from in idual piglets in a subset of litters was assessed at three time points (16 and 19 days of age and at weaning), allowing the characterisation of in idual pigs as good, moderate, small or non-eaters. There was no difference between treatment groups (χ2 = 6.27, P = 0.71) in the characterisation of apparent creep feed disappearance by in idual piglets, however piglets offered the simple creep diet before weaning consumed more feed (P = 0.004) and gained weight faster (P = 0.007) during the first 5 days after weaning than those animals offered the complex creep diet. Feed intake from weaning to 49 days of age also tended to be greater in pigs offered the simple creep diet before weaning (P = 0.053), with this difference reflected in daily gain from weaning to 49 days of age (P = 0.051). Despite these differences, creep feed composition did not influence lifetime growth performance or carcass composition. Pigs weaned at 22 days of age were heavier midway through the weaner period (49 days of age) than pigs weaned at 29 days of age (15.2 versus 14.3 kg, respectively, P = 0.009), and tended to grow faster from birth to slaughter (616 versus 610 g/day, respectively, P = 0.079). These data suggest that the provision of a complex creep diet during lactation may not improve lifetime growth performance above that of a less expensive creep diet. Weaning age may however influence lifetime performance, with weaning at 29 days of age tending to reduce rate of gain from birth to slaughter.

Rams with poor feed efficiency are highly responsive to an exogenous adrenocorticotropin hormone (ACTH) challenge

Publisher: Elsevier BV

Date: 04-2008

DOI: 10.1016/J.DOMANIEND.2007.07.002

Abstract: An animal's response to a stressor is to increase metabolic rate, and thus energy consumption through the activation of the hypothalamic-pituitary-adrenal axis. Changes to energy use by an animal are likely to influence the efficiency with which it is utilised. In this study, we tested the hypothesis that less efficient sheep are more responsive to exogenous administration of adrenocorticotropin hormone. This was done by firstly determining the appropriate dose (0.4, 1.6 or 6.4microg/kg LW) and peak serum cortisol response time (45min) to exogenous administration of adrenocorticotropin hormone in a pilot study (n=3 sheep). Following this, adrenocorticotropin hormone (2.0microg/kg LW) stimulated cortisol levels were measured in a larger group of sheep (n=50) of known feed efficiency (feed conversion ratio and residual feed intake values). Less efficient sheep (more positive residual feed intake values) were found to have a greater (P<0.001) increase in cortisol concentration in comparison to more efficient animals. Those sheep which had higher levels of cortisol also had a greater proportion (P<0.001) of fat tissue. These data clearly demonstrated that efficiency of energy use, when measured as residual feed intake, is significantly related to an animal's stress response. These findings have important implications for understanding the physiological mechanisms underpinning efficiency of energy use, and may be useful in successfully identifying animals which are superior in terms of feed efficiency.

Nano-chromium picolinate and heat stress enhance insulin sensitivity in cross-bred sheep

Publisher: Elsevier BV

Date: 06-2023

DOI: 10.1016/J.ANINU.2023.01.003

Adaptive and Productive Sheep Breed for Changing Climate

Publisher: MDPI

Date: 04-2020

DOI: 10.3390/PROCEEDINGS2019036137

Dual energy X-ray absorptiometry (DXA) can be used to predict live animal and whole carcass composition of sheep

Publisher: Elsevier BV

Date: 10-2011

DOI: 10.1016/J.SMALLRUMRES.2011.07.003

Interrelationships between porcine somatotropin (pST), betaine, and energy level on body composition and tissue distribution of finisher boars

Publisher: CSIRO Publishing

Date: 2004

DOI: 10.1071/AR04029

Abstract: Fifty-six in idually penned boars (initial weight 64 kg) were used to investigate the interactions between dietary betaine, dietary energy, and porcine somatotropin (pST) treatment. The study was a 2 × 2 × 2 factorial experiment with the respective factors being dietary betaine (0 or 1.5 g/kg) and energy level (80% or 100% ad libitum) and treatment with pST (0 or 5 mg/day). A Hologic QDR4500A Dual Energy X-ray Absorptiometer (DXA) was used to determine body composition of pigs at the beginning and end of the study at Day 35. After slaughter, the composition of the whole half-carcass as well as the shoulder, ham, belly, and loin primal cuts was determined with DXA and verified with manual dissection. The main effects of dietary betaine were most pronounced when dietary energy was restricted. Under these conditions, daily gain was increased by dietary betaine (1188 v. 1271 g/day, P = 0.049) and pST (1115 v. 1344 g/day, P 0.001). When dietary energy was restricted, lean tissue deposition was increased by dietary betaine (830 v. 908 g/day, P = 0.032) and pST (764 v. 974 g/day, P 0.001), and these effects were additive. As a result, the lean meat yield in the half-carcass was increased by both dietary betaine (23.9 v. 25.1 kg, P = 0.043) and pST (23.3 v. 25.7 kg, P 0.001). Lean tissue responses in primal cuts were more variable but followed a similar pattern. There was little effect of either dietary betaine or pST on fat deposition. These data demonstrate that when energy intake is limiting the potential for growth (as is normally the case for the improved boar), then both dietary betaine and pST treatment, either alone or in combination, can increase lean tissue deposition without increasing fat deposition.

Digital Integration and Automated Assessment of Eye-Tracking and Emotional Response Data Using the BioSensory App to Maximize Packaging Label Analysis

Publisher: MDPI AG

Date: 17-11-2021

DOI: 10.3390/S21227641

Abstract: New and emerging non-invasive digital tools, such as eye-tracking, facial expression and physiological biometrics, have been implemented to extract more objective sensory responses by panelists from packaging and, specifically, labels. However, integrating these technologies from different company providers and software for data acquisition and analysis makes their practical application difficult for research and the industry. This study proposed a prototype integration between eye tracking and emotional biometrics using the BioSensory computer application for three s le labels: Stevia, Potato chips, and Spaghetti. Multivariate data analyses are presented, showing the integrative analysis approach of the proposed prototype system. Further studies can be conducted with this system and integrating other biometrics available, such as physiological response with heart rate, blood, pressure, and temperature changes analyzed while focusing on different label components or packaging features. By maximizing data extraction from various components of packaging and labels, smart predictive systems can also be implemented, such as machine learning to assess liking and other parameters of interest from the whole package and specific components.

Neonatal oxytocin administration and supplemental milk ameliorate the weaning transition and alter hormonal expression in the gastrointestinal tract in pigs

Publisher: Elsevier BV

Date: 04-2015

DOI: 10.1016/J.DOMANIEND.2014.11.001

Abstract: The aim of this study was to investigate the influences of milk supplementation during lactation, over 1 wk after weaning, and oxytocin administration for the first 14 d of life on the pigs' response to weaning. Pigs from 20 litters were allocated to each of these 3 treatments in a randomized factorial design. Oxytocin was administered subcutaneously daily from 0 to 14 d of age at a rate of 10 I.U. per kg. The milk supplement consisted of a mixture of 25% skim milk powder offered either during lactation between 10 and 20 d of age or for the first week after weaning as a transitional diet along with dry pellets. Pigs were weaned at 21 d of age. Growth rate was measured from birth to slaughter at 140 d of age and feed intake of supplemental milk or feed from 10 to 56 d of age. Organ weights (heart, liver, stomach, and kidneys) and the gene expression of ghrelin, leptin, and glucagon-like peptides (glucagon-like peptide-1 and glucagon-like peptide-2) were measured in the stomach, ileum, and duodenum at 10, 21, and 28 d of age. Milk supplementation after weaning resulted in immediate feed intake and partially alleviated the depression in growth rate over the first 7 d postweaning (P 0.1). However, effects were only transient and disappeared once the milk liquid diet was removed. Neonatal oxytocin administration reduced weight loss over the first 2 d after weaning (P = 0.03), without affecting feed intake (P > 0.1), hence possibly reducing weaning stress. Seven days after weaning, oxytocin-treated pigs had greater stomach ghrelin and leptin expression (both P = 0.02), and pigs supplemented with milk after weaning had greater stomach leptin and glucagon-like peptide-2 expression (P = 0.02 and P = 0.05, respectively). Hence, neonatal oxytocin administration or postweaning milk supplementation are both effective means of enhancing gastric leptin expression and reducing weight loss at weaning, likely improving gut health during this critical period.

Biometric Physiological Responses from Dairy Cows Measured by Visible Remote Sensing Are Good Predictors of Milk Productivity and Quality through Artificial Intelligence

Publisher: MDPI AG

Date: 14-10-2021

DOI: 10.3390/S21206844

Abstract: New and emerging technologies, especially those based on non-invasive video and thermal infrared cameras, can be readily tested on robotic milking facilities. In this research, implemented non-invasive computer vision methods to estimate cow’s heart rate, respiration rate, and abrupt movements captured using RGB cameras and machine learning modelling to predict eye temperature, milk production and quality are presented. RGB and infrared thermal videos (IRTV) were acquired from cows using a robotic milking facility. Results from 102 different cows with replicates (n = 150) showed that an artificial neural network (ANN) model using only inputs from RGB cameras presented high accuracy (R = 0.96) in predicting eye temperature (°C), using IRTV as ground truth, daily milk productivity (kg-milk-day−1), cow milk productivity (kg-milk-cow−1), milk fat (%) and milk protein (%) with no signs of overfitting. The ANN model developed was deployed using an independent 132 cow s les obtained on different days, which also rendered high accuracy and was similar to the model development (R = 0.93). This model can be easily applied using affordable RGB camera systems to obtain all the proposed targets, including eye temperature, which can also be used to model animal welfare and biotic/abiotic stress. Furthermore, these models can be readily deployed in conventional dairy farms.

Impact of Rumen Fluid Storage on In Vitro Feed Fermentation Characteristics

Publisher: MDPI AG

Date: 19-04-2023

DOI: 10.3390/FERMENTATION9040392

Abstract: Storing rumen fluid (RF) has the potential to standardize subsequent in vitro feed fermentation studies. The first phase of this experiment aimed to evaluate the effect of two RF storage methods on gas composition and dry matter disappearance (DMD) in wheat grain and lucerne hay under in vitro fermentation. The storage methods were as follows: (1) snap-freezing RF using liquid nitrogen and then storing it at −80 °C (−80 °C) and (2) mixing RF with 5% dimethyl sulfoxide (DMSO), subsequently freezing it at −20 °C (D−20 °C), and comparing it to fresh RF on days 1, 14, and 30 post collection. The objective of the second phase was to quantify the impact of preserving the RF for 180 days at D−20 °C on the in vitro fermentation parameters. The methane composition was lower (p 0.001) in both the preserved RFs than in the fresh RF. There was no difference (p 0.05) in DMD values between days 14 and 30. The average cumulative gas production and DMD from the RF stored at D−20 °C was higher than that from the RF stored at −80 °C. Moreover, there was no difference between day 30 and day 180 in the total gas production and lag time when fermenting with RF preserved at D−20 °C. Therefore, storing RF at D−20 °C is preferable to storing it at −80 °C when access to fresh RF is limited.

Amelioration of thermal stress impacts in dairy cows

Publisher: CSIRO Publishing

Date: 2013

DOI: 10.1071/AN12384

Abstract: Heat stress negatively impacts on a variety of animal production parameters. Advances in management strategies have alleviated some of the negative impacts of thermal stress on farm animals, but production continues to markedly decrease during heat events in summer, particularly in dairy cattle. In this paper we introduce a Dairy Risk Assessment Program (DRAP). The DRAP is a user-friendly software package designed to assist users in predicting heat loads in dairy cow herds. DRAP was developed over three Australian summers using climatic data (temperature, humidity, solar radiation and wind speed), cow production data (milk yield and milk quality), and physiological data (respiration rate and body temperature). The data were used to develop mathematical algorithms which can predict animal response to climatic variables. This software package is designed to be used by the dairy industry to better manage cows during times of elevated environmental temperatures by equipping producers, managers, and dairy industry personnel with Dairy Heat Load Index (DHLI) values which were calculated based upon site information, stock characteristics, management practices, and mitigation variables specific to their dairy production unit. When a heat event is imminent producers can then introduce management strategies such as providing shade or additional water troughs or implementation of nutritional strategies. Some of these nutritional strategies include dietary chromium picolinate, betaine and antioxidant supplementation or altering the rate of starch fermentation. These nutritional strategies are discussed at some length in this paper.

Effects of nutrition and management on the production and composition of milk fat and protein: a review

Publisher: CSIRO Publishing

Date: 2004

DOI: 10.1071/AR03173

Abstract: The composition and functional properties of cow’s milk are of considerable importance to the dairy farmer, manufacturer, and consumer. Broadly, there are 3 options for altering the composition and/or functional properties of milk: cow nutrition and management, cow genetics, and dairy manufacturing technologies. This review considers the effects of nutrition and management on the composition and production of milk fat and protein, and the relevance of these effects to the feeding systems used in the Australian dairy industry. Dairy cows on herbage-based diets derive fatty acids for milk fat synthesis from the diet/rumen microorganisms (400–450 g/kg), from adipose tissues ( g/kg), and from de novo synthesis in the mammary gland (about 500 g/kg). However, the relative contributions of these sources of fatty acids to milk fat production are highly dependent upon feed intake, diet composition, and stage of lactation. Feed intake, the amount of starch relative to fibre, the amount and composition of long chain fatty acids in the diet, and energy balance are particularly important. Significant differences in these factors exist between pasture-based dairy production systems and those based on total mixed ration, leading to differences in milk fat composition between the two. High intakes of starch are associated with higher levels of de novo synthesis of fat in the mammary gland, resulting in milk fat with a higher concentration of saturated fatty acids. In contrast, higher intakes of polyunsaturated fatty acids from pasture and/or lipid supplements result in higher concentrations of unsaturated fatty acids, particularly oleate, trans-vaccenate, and conjugated linoleic acid (CLA) in milk fat. A decline in milk fat concentration associated with increased feeding with starch-based concentrates can be attributed to changes in the ratios of lipogenic to glucogenic volatile fatty acids produced in the rumen. Milk fat depression, however, is likely the result of increased rates of production of long chain fatty acids containing a trans-10 double bond in the rumen, in particular trans-10 18 : 1 and trans-10-cis-12 18 : 2 in response to diets that contain a high concentration of polyunsaturated fatty acids and/or starch. Low rumen fluid pH can also be a factor. The concentration and composition of protein in milk are largely unresponsive to variation in nutrition and management. Exceptions to this are the effects of very low intakes of metabolisable energy (ME) and/or metabolisable protein (MP) on the concentration of total protein in milk, and the effects of feeding with supplements that contain organic Se on the concentration of Se, as selenoprotein, in milk. In general, the first limitation for the synthesis of milk protein in Australian dairy production systems is availability of ME since pasture usually provides an excess of MP. However, low concentrations of protein in milk produced in Queensland and Western Australia, associated with seasonal variations in the nutritional value of herbage, may be a response to low intakes of both ME and MP. Stage of lactation is important in determining milk protein concentration, but has little influence on protein composition. The exception to this is in very late lactation where stage of lactation and low ME intake can interact to reduce the casein fraction and increase the whey fraction in milk and, consequently, reduce the yield of cheese per unit of milk. Milk and dairy products could also provide significant amounts of Se, as selenoproteins, in human diets. Feeding organic Se supplements to dairy cows grazing pastures that are low in Se may also benefit cow health. Research into targetted feeding strategies that make use of feed supplements including oil seeds, vegetable and fish oils, and organic Se supplements would increase the management options available to dairy farmers for the production of milks that differ in their composition. Given appropriate market signals, milk could be produced with lower concentrations of fat or higher levels of unsaturated fats, including CLA, and/or high concentrations of selenoproteins. This has the potential to allow the farmer to find a higher value market for milk and improve the competitiveness of the dairy manufacturer by enabling better matching of the supply of dairy products to the demands of the market.

Longitudinal DXA measurements demonstrate lifetime differences in lean and fat tissue deposition between boars and barrows under individual and group-penned systems

Publisher: CSIRO Publishing

Date: 2006

DOI: 10.1071/AR04266

Abstract: Thirty-two Large White × Landrace male pigs were used to determine the relationships between the rates of tissue deposition and age, in boars and barrows under 2 housing systems. A 2 × 2 factorial design was used, with the respective treatments being sex (boar or barrow) and housing condition (in idually penned system or group-penned system). Surgical castration was performed at 7 days of age. In idually housed pigs were used to provide an estimate of potential growth and were weaned at 10 days into in idual cages and provided with supplemental fermented skim milk for 2 weeks. Group-housed pigs were weaned at 24 days of age and reared in group pens of boars and castrates typical for commercial production. Conventional weaner, grower, and finisher diets were provided ad libitum to all pigs from weaning onwards. A Hologic QDR4500A dual-energy X-ray absorptiometer (DXA) was used to determine lean, fat, and bone composition at 4-weekly intervals from 10 until 150 days of age. Over the 20 weeks of the study, boars deposited less fat than barrows (136 v. 179 g/day, s.e.d. = 6.49, P 0.001). Over the same time frame, boars deposited more lean tissue than barrows when in idually penned (490 v. 444 g/day, s.e.d. = 14.4, P 0.05) but there was no difference in group-penned pigs (464 v. 443 g/day, s.e.d. = 14.4, P 0.1). This was more pronounced in the final 4 weeks of growth when in idually penned boars deposited 200 g/day more lean than barrows (P 0.001), with no increase in group-penned animals. However, during this growth phase, group penning further increased the fat deposition margin between boars and barrows where boars deposited 90 g/day less fat when in idually penned (P 0.001), but 140 g/day less fat (P 0.001) when group penned. The results show that the advantages of boars in terms of growth and lean tissue composition are substantially reduced in group-penned situations. However, because of fat deposition, boars retain some advantage over barrows in group-penned systems at liveweights over about 50 kg. These data may aid in the accurate prediction of the nutrient requirements and optimum slaughter weight for barrows.

Reducing the length of time between slaughter and the secondary gonadotropin-releasing factor immunization improves growth performance and clears boar taint compounds in male finishing pigs1

Publisher: Oxford University Press (OUP)

Date: 09-2011

DOI: 10.2527/JAS.2010-3267

Abstract: The objective of this study was to evaluate whether altering the timing of the secondary anti-gonadotropin-releasing factor (GnRF) immunization closer to slaughter in male finishing pigs would reduce the increase in P2 fat depth (6.5 cm from the midline over the last rib), while still limiting the incidence of boar taint. Entire male pigs are immunized against GnRF to reduce the concentration of testicular steroids that in turn limits the incidence of boar taint. Additionally, testicle measurements and color measurements were taken to examine whether they could be used to differentiate nonimmunized entire males from immunized male pigs. A total of 175 Large White × Landrace entire male pigs aged 16 wk (59 kg of BW) were used in a completely randomized design with 5 treatment groups based on the time that pigs received the secondary immunization before slaughter. Pigs were housed in groups of 7 and randomly allocated to 1 of 5 treatments with 5 replicates per treatment. The treatment groups were as follows: no secondary immunization before slaughter, and the secondary immunization given at 2, 3, 4, or 6 wk before slaughter. The P2 fat depth levels were reduced (P = 0.054) with the secondary immunization closer to slaughter (11.7, 11.3, 12.8, 12.6, and 13.7 mm for no secondary immunization, secondary immunization at 2, 3, 4, and 6 wk before slaughter, respectively). Androstenone concentration did not exceed the generally accepted industry sensory threshold of 1.0 µg/g of fat, and both androstenone concentration in the adipose tissue and testosterone concentrations in the blood were suppressed (P < 0.001) in all immunized pigs regardless of timing of the secondary immunization compared with pigs that did not receive the secondary immunization. Skatole concentration of all pigs in the experiment did not exceed the generally accepted industry sensory threshold of 0.2 µg/g. Testes weight was reduced (P < 0.001) with increased time between slaughter and the secondary immunization. Immunized pigs, regardless of time before slaughter, had greater L* (lightness) and b* (yellowness) color of the testicle surface (P < 0.001 and P = 0.020, respectively), and less a* (redness) color compared with entire males (P < 0.001). The study provides further evidence of the efficacy of the anti-GnRF immunization and indicates that the secondary immunization can be moved closer to slaughter, while still limiting the incidence of boar taint. Testicle measurements and color measurements together could provide a method of discrimination between carcasses from immunized entire males clear of boar taint and tainted carcasses.

Heat Stress Impacts on Lactating Cows Grazing Australian Summer Pastures on an Automatic Robotic Dairy

Publisher: MDPI AG

Date: 17-05-2020

DOI: 10.3390/ANI10050869

Abstract: The objective of this study was to measure the impacts of summer heat events on physiological parameters (body temperature, respiratory rate and panting scores), grazing behaviour and production parameters of lactating Holstein Friesian cows managed on an Automated Robotic Dairy during Australian summer. The severity of heat stress was measured using Temperature-Humidity Index (THI) and impacts of different THIs—low (≤72), moderate (73–82) and high (≥83)—on physiological responses and production performance were measured. There was a highly significant (p ≤ 0.01) effect of THI on respiratory rate (66.7, 84.7 and 109.1/min), panting scores (1.4, 1.9 and 2.3) and average body temperature of cows (38.4, 39.4 and 41.5 °C), which increased as THI increased from low to moderate to high over the summer. Average milk production parameters were also significantly (p ≤ 0.01) affected by THI, such that daily milk production dropped by 14% from low to high THI, milk temperature and fat% increased by 3%, whilst protein% increased by 2%. The lactation stage of cow had no significant effect on physiological parameters but affected (p ≤ 0.05) average daily milk yield and milk solids. Highly significant (p ≤ 0.01) positive correlations were obtained between THI and milk temperature, fat% and protein% whilst the reverse was observed between THI and milk yield, feed intake and rumination time. Under moderate and high THI, most cows sought shade, spent more time around watering points and showed signs of distress (excessive salivation and open mouth panting). In view of the expected future increase in the frequency and severity of heat events, additional strategies including selection and breeding for thermotolerance and dietary interventions to improve resilience of cows need to be pursued.

Phenolic bioaccessibility, antioxidant, and antidiabetic effects of indigenous fermented coffee beans after simulated gastrointestinal digestion and colonic fermentation

Publisher: Elsevier BV

Date: 08-2023

DOI: 10.1016/J.FBIO.2023.102920

Genotype and age effects on sheep meat production. 2. Carcass quality traits

Publisher: CSIRO Publishing

Date: 2007

DOI: 10.1071/EA07001

Abstract: The present study examines carcass quality traits in different genotypes of Australian sheep slaughtered at 4, 8, 14 and 22 months of age. Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Merino × Merino (M × M) and Border Leicester × Merino (BL × M) genotypes were compared. Sires were selected on the basis of growth (PDg, M and BL) and muscle (PDm) using estimated breeding values (EBVs). Approximately 150 mixed sex animals were slaughtered at each age (595 in total for the four slaughters) and carcass quality traits, including hot carcass weight, loin and semitendinosus (ST) muscle weights, GR fat, fat C, rump fat depth and eye muscle area (EMA), were determined at 45 min to 3 days postslaughter. With age, all carcass quality traits increased in magnitude. Merinos produced the lightest and leanest carcasses (P 0.01) with lowest values in nearly all the carcass quality traits. At 4 and 8 months of age carcass and loin weights, EMA and GR fat measures were greater (P 0.01) in second-cross PDg × BLM sheep than all other genotypes. However, traits related to muscle content were similar between first- and second-cross Poll Dorset genotypes at 14 and 22 months of age. The BL × M genotype had lower values than the PD × M genotypes for these traits, at all ages. Traits related to carcass fatness showed that sheep with Border Leicester genetics tend to deposit more fat (P 0.001) in the carcass than those with Merino or Poll Dorset genetics from 14 months of age onwards. Most carcass quality traits did not differ (P 0.05) between first-cross Poll Dorset genotypes selected for growth or muscling (PDg × M v. PDm × M). Sires with high muscling EBVs are associated more with shorter carcass length than low muscling EBVs. From ~8 months of age, wethers had greater values (P 0.05) than ewes of the same age for most carcass quality traits related to size and muscle development, but no effect was present for carcass quality traits that were related to fat deposition. It was clear that the carcass weight of M × M at 8 and 14 months were similar to that observed in PDg × BLM at 4 and 8 months, respectively. Loin weights of M × M and BL × M at 13 and 8 months were similar to that weights observed in PDg × BLM at 8 and 4 months of age, respectively. Animals slaughtered to a muscle weight specification will reach the target at a younger age when they have more Poll Dorset than Border Leicester background genetics. Alternatively animals slaughtered at a specified fat score (using fat C or rump fat depth) will reach the specification at a younger age when they have more Border Leicester than Poll Dorset genetics.

Resilience of small ruminants to climate change and increased environmental temperature: A review

Publisher: MDPI AG

Date: 17-05-2020

DOI: 10.3390/ANI10050867

Abstract: Climate change is a major global threat to the sustainability of livestock systems. Climatic factors such as ambient temperature, relative humidity, direct and indirect solar radiation and wind speed influence feed and water availability, fodder quality and disease occurrence, with production being most efficient in optimal environmental conditions. Among these climatic variables, ambient temperature fluctuations have the most impact on livestock production and animal welfare. Continuous exposure of the animals to heat stress compromises growth, milk and meat production and reproduction. The capacity of an animal to mitigate effects of increased environmental temperature, without progressing into stress response, differs within and between species. Comparatively, small ruminants are better adapted to hot environments than large ruminants and have better ability to survive, produce and reproduce in harsh climatic regions. Nevertheless, the physiological and behavioral changes in response to hot environments affect small ruminant production. It has been found that tropical breeds are more adaptive to hot climates than high-producing temperate breeds. The growing body of knowledge on the negative impact of heat stress on small ruminant production and welfare will assist in the development of suitable strategies to mitigate heat stress. Selection of thermotolerant breeds, through identification of genetic traits for adaption to extreme environmental conditions (high temperature, feed scarcity, water scarcity), is a viable strategy to combat climate change and minimize the impact on small ruminant production and welfare. This review highlights such adaption within and among different breeds of small ruminants challenged by heat stress.

Emerging Technologies Based on Artificial Intelligence to Assess the Quality and Consumer Preference of Beverages

Publisher: MDPI AG

Date: 11-2019

DOI: 10.3390/BEVERAGES5040062

Abstract: Beverages is a broad and important category within the food industry, which is comprised of a wide range of sub-categories and types of drinks with different levels of complexity for their manufacturing and quality assessment. Traditional methods to evaluate the quality traits of beverages consist of tedious, time-consuming, and costly techniques, which do not allow researchers to procure results in real-time. Therefore, there is a need to test and implement emerging technologies in order to automate and facilitate those analyses within this industry. This paper aimed to present the most recent publications and trends regarding the use of low-cost, reliable, and accurate, remote or non-contact techniques using robotics, machine learning, computer vision, biometrics and the application of artificial intelligence, as well as to identify the research gaps within the beverage industry. It was found that there is a wide opportunity in the development and use of robotics and biometrics for all types of beverages, but especially for hot and non-alcoholic drinks. Furthermore, there is a lack of knowledge and clarity within the industry, and research about the concepts of artificial intelligence and machine learning, as well as that concerning the correct design and interpretation of modeling related to the lack of inclusion of relevant data, additional to presenting over- or under-fitted models.

Evaluation of Sugarcane-Derived Polyphenols on the Pre-Weaning and Post-Weaning Growth of Gilt Progeny

Publisher: MDPI AG

Date: 05-06-2020

DOI: 10.3390/ANI10060984

Abstract: Gilt progeny (GP) exhibit poorer growth compared with sow progeny (SP), particularly in the pre-weaning and post-weaning period. Late gestation/lactation sow diets and weaner diets were supplemented with 0.5% Polygain (POL), a sugarcane extract rich in polyphenols, to collectively improve GP growth in these periods. Gilts (n = 60) and sows (n = 68, parities 2 and 3) were fed a control or POL diet. Weaned GP (n = 79) and SP (n = 92) born to these dams were also fed either a CON or POL diet. Gilts litters weighed less than sow litters at birth and 21 days (p 0.001 for both) and were not improved by POL (p = 0.80 and 0.54, respectively). GP were lighter than SP at day 7 and day 14 post-weaning (p 0.001 for both) and were not improved by the POL diet at these timepoints (p = 0.61 and 0.97, respectively). Plasma interleukin-1β (IL-1β) was increased at weaning despite POL supplementation (p = 0.022) and GP had reduced IL-1β overall (p = 0.021). Overall, POL was unable to improve growth in GP and the attenuated immune response seen in GP could be contributing to their poor growth performance.

Dietary conjugated linoleic acid differentially alters fatty acid composition and increases conjugated linoleic acid content in porcine adipose tissue

Publisher: Cambridge University Press (CUP)

Date: 11-2003

DOI: 10.1079/BJN2003982

Abstract: Conjugated linoleic acids (CLA) have been shown to decrease body fat content in pigs. It is possible that feeding pigs diets rich in CLA may increase carcass lipid CLA to levels that could provide health benefits when included as a part of a healthy diet. Therefore, the aim of the present study was to determine whether dietary CLA supplementation has any effect on the fatty acid composition of subcutaneous and intramuscular adipose tissue in pigs. Thirty-five female cross bred (Large White×Landrace) pigs (initial weight 57·2kg and initial P2 back fat 11·5mm) were used in the present study. Pigs were housed in idually and randomly allocated to one of six dietary treatments (0·00, 1·25, 2·50, 5·00, 7·50 and 10·00g CLA55 (55g CLA isomers/100g total fatty acids Natural Lipids Ltd, Hovdebygda, Norway)/kg) and fed their respective diets for 8 weeks. Twelve CLA isomers in the diet and in pig tissue lipids were separated by Ag + -HPLC. CLA was incorporated at fivefold higher levels in subcutaneous fat as compared with intramuscular fat and in a dose-dependant manner. Overall, the transfer efficiency of CLA was maximized at 5·00g CLA55/kg. However, there was clear selectivity in the uptake or incorporation of cis , trans -9,11 isomer over the trans , cis -10,12 isomer. In general, CLA supplementation produced significant changes in skeletal muscle and adipose tissue fatty acid composition, indicating that dietary CLA had a potent affect on lipid transport and metabolism in vivo . Significant increases in myristic, palmitic and palmitoleic acids and a reduction in arachidonic acid were observed, suggesting an alteration in activity of Δ 5 -, Δ 6 - and Δ 9 -desaturases in pig adipose tissue. In conclusion, feeding pigs diets supplemented with CLA increases carcass lipid CLA, but also results in changes in the fatty acid profile in pig fat that could potentially outweigh the benefits of CLA.

Betaine Improves Milk Yield in Grazing Dairy Cows Supplemented with Concentrates at High Temperatures

Publisher: MDPI AG

Date: 13-02-2019

DOI: 10.3390/ANI9020057

Abstract: Betaine is an organic osmolyte sourced from sugar beet that accumulates in plant cells undergoing osmotic stress. Since the accumulation of betaine lowers the energy requirements of animals and, therefore, metabolic heat production, the aim of this experiment was to investigate if betaine supplementation improved milk yield in grazing dairy cows in summer. One hundred and eighteen Friesian × Holstein cows were paired on days in milk and, within each pair, randomly allocated to a containing treatment of either 0 or 2 g/kg natural betaine in their concentrate ration for approximately 3 weeks during February/March 2015 (summer in Australia). The mean maximum February temperature was 30 °C. Cows were allocated approximately 14 kg dry matter pasture and 7.5 kg of concentrate pellets (fed in the milking shed) per cow per day and were milked through an automatic milking system three times per day. Betaine supplementation increased average daily milk yield by over 6% (22.0 vs. 23.4 kg/day, p 0.001) with the response increasing as the study progressed as indicated by the interaction (p 0.001) between betaine and day. Milk fat % (p = 0.87), milk protein % (p = 0.90), and milk somatic cell count (p = 0.81) were unchanged by dietary betaine. However, betaine supplementation increased milk protein yield (677 vs. 719 g/day, p 0.001) and fat yield (874 vs. 922 g/day, p 0.001) with responses again being more pronounced as the study progressed. In conclusion, dietary betaine supplementation increased milk and component yield during summer in grazing dairy cows.

Extra‐virgin and refined olive oils decrease plasma triglyceride, moderately affect lipoprotein oxidation susceptibility and increase bone density in growing pigs

Publisher: Wiley

Date: 17-07-2006

DOI: 10.1002/JSFA.2569

Estimating the impact of various pathway parameters on tenderness, flavour and juiciness of pork using Monte Carlo simulation methods

Publisher: Elsevier BV

Date: 06-2016

DOI: 10.1016/J.MEATSCI.2016.01.004

Abstract: Monte Carlo simulation was investigated as a potential methodology to estimate sensory tenderness, flavour and juiciness scores of pork following the implementation of key pathway interventions known to influence eating quality. Correction factors were established using mean data from published studies investigating key production, processing and cooking parameters. Probability distributions of correction factors were developed for single pathway parameters only, due to lack of interaction data. Except for moisture infusion, ageing period, aitchbone hanging and cooking pork to an internal temperature of >74°C, only small shifts in the mean of the probability distributions of correction factors were observed for the majority of pathway parameters investigated in this study. Output distributions of sensory scores, generated from Monte Carlo simulations of input distributions of correction factors and for in idual pigs, indicated that this methodology may be useful in estimating both the shift and variability in pork eating traits when different pathway interventions are applied.

Genotype and age effects on sheep meat production 4. Carcass composition predicted by dual energy X-ray absorptiometry

Publisher: CSIRO Publishing

Date: 2007

DOI: 10.1071/EA07008

Abstract: This study compares the carcass composition of different genotypes of Australian sheep slaughtered at four ages (110, 236, 412 and 662 days of age, or 4, 8, 14 and 22 months of age). The genotypes used were Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Merino × Merino (M × M) and Border Leicester × Merino (BL × M). Approximately 150 mixed sex animals were slaughtered at each age and the right sides (half carcass) were scanned by dual energy X-ray absorptiometry (DXA) for chemical lean, fat and bone mineral percentages of the carcass. Carcass lean percentage decreased (P 0.001) across sire groups from Merino to Poll Dorset to Border Leicester at each age, while fatness increased (P 0.001) in the opposite direction. With age, the magnitude of difference between genotypes for fatness became greater. There was a marked increase (P 0.01) in ash mineral percentages in M × M and PDg × M genotypes relative to other genotypes in the 14- and 22-month groups. There were no differences (P 0.1) between PDg × M and PDm × M in either chemical lean or fat percentages at any age but ash mineral percentage was higher in the PDg × M genotype at older ages (P 0.05). This in turn led to differences in chemical lean : ash mineral content at later ages between PDg × M and PDm × M. Wethers had higher chemical lean (P 0.05) and lower fat (P 0.05) percentages in the carcass than ewes at all ages but ash mineral and chemical lean : ash mineral content did not differ between sexes. It was clear that the level of chemical lean or fat or ash mineral composition reached by some genotypes at one age was equivalent to that reached by other genotypes at the next measurement age. Those genotypes that reach a specification at younger ages will be more productive for meat production. Purebred Merino genetics will always be less productive in terms of carcass weight and muscle related productive traits (loin weight, semitendinosus muscle weight, eye muscle area) than BL and PD genetics, but the relative productivity of PD and BL genetics will depend on the traits used in the specification [see also Ponn alam EN, Hopkins DL, Butler KL, Dunshea FR, Warner RD (2007) Genotype and age effects on sheep meat production. 2. Carcass quality traits. Australian Journal of Experimental Agriculure 47, 1147–1154]. The results suggest that selection for muscling had a greater impact on reducing ash mineral content associated with bone mineral mass than on increasing lean mass in the carcass.

Responses to homeostatic signals in ractopamine-treated pigs

Publisher: Cambridge University Press (CUP)

Date: 06-1995

DOI: 10.1079/BJN19950086

Abstract: The β-agonist ractopamine (RAC) promotes protein deposition with little effect on fat deposition in the pig. To assess whether the lack of effect on fat deposition was due to changes in response to homeostatic signals, eight crossbred gilts (73 kg body weight (BW)) with venous catheters were used to examine plasma metabolite and hormone concentrations before and after intravenous injections of insulin and the β 2 -agonist fenoterol during dietary RAC (0 or 20 mg/kg) treatment. Pigs received intravenous challenges of insulin (1μg/kg BW) on days 3, 9 and 23 and fenoterol (2 μg/kg BW) on days 4, 10 and 24 of treatment. RAC was then withdrawn from the diet and insulin and fenoterol challenges were repeated 6 and 7 d later respectively. Blood s les for the determination of metabolite and hormone concentrations were taken at -30, -20, -10, -1, 2·5, 5, 10, 20, 30, 45, 60 and 120 min relative to the challenges. Dietary RAC decreased basal plasma insulin concentrations but had no effect on plasma glucose or non-esterified fatty acids (NEFA). Hypoglycaemic responses to insulin were not affected by RAC while the anti-lipolytic effects of insulin tended to be augmented. Dietary RAC decreased the lipolytic response to fenoterol, this being evident after 4 d treatment. Hypoglycaemic response to fenoterol was not changed whereas the hyperinsulinaemic response to fenterol was attenuated by dietary RAC. Previous treatment with RAC did not influence basal hormone and metabolite concentrations or responses to homeostatic signals during the withdrawal period. While these results suggest little change in glucose metabolism, the de-sensitization of adipose tissue β-adrenergic receptors is consistent with the observations that dietary RAC has little effect on the rate of fat deposition in the growing pig.

Artificially extending photoperiod improves milk yield in dairy goats and is most effective in late lactation

Publisher: Elsevier BV

Date: 06-2013

DOI: 10.1016/J.SMALLRUMRES.2013.01.002

A Meta-Analysis of the Effectiveness of High, Medium, and Low Voltage Electrical Stimulation on the Meat Quality of Small Ruminants

Publisher: MDPI AG

Date: 02-11-2020

DOI: 10.3390/FOODS9111587

Abstract: The current study is a meta-analysis of the effects of electrical stimulation (ES, n = 28 papers) with different voltages combined with different ageing periods (0–3, 4–7, and 8–14 days) on the meat quality of small ruminants. A comprehensive search for published studies on meat quality of small ruminants investigating the application of low, medium, and high voltage electrical stimulation, was performed using Google Scholar, ScienceDirect, PubMed, and Scopus databases. Forest plots, funnel plots, and other statistical tools and tests were used in the study to analyze the results. Electrical stimulation significantly reduced ultimate pH (p 0.001), Warner–Bratzler shear force (WBSF) (p 0.001), cooking loss (p 0.05), and purge loss (p 0.001). In addition, sarcomere length (p 0.01), myofibrillar-fragmentation index (MFI) (p 0.001), and color (L*, a*, b*) (p 0.001) showed higher values in meat subjected to ES as compared with the control group. In conclusion, the meta-analysis revealed statistical proof of beneficial effects of ES on meat quality of small ruminants in terms of ultimate pH, tenderness, enhanced proteolysis, and higher colorimetric values.

A Meta-Analysis of Zilpaterol and Ractopamine Effects on Feedlot Performance, Carcass Traits and Shear Strength of Meat in Cattle

Publisher: Public Library of Science (PLoS)

Date: 30-12-2014

DOI: 10.1371/JOURNAL.PONE.0115904

Valorization of Food Waste to Produce Value-Added Products Based on Its Bioactive Compounds

Publisher: MDPI AG

Date: 10-03-2023

DOI: 10.3390/PR11030840

Abstract: The rapid growth of the global population and changes in lifestyle have led to a significant increase in food waste from various industrial, agricultural, and household sources. Nearly one-third of the food produced annually is wasted, resulting in severe resource depletion. Food waste contains rich organic matter, which, if not managed properly, can pose a serious threat to the environment and human health, making the proper disposal of food waste an urgent global issue. However, various types of food waste, such as waste from fruit, vegetables, grains, and other food production and processing, contain important bioactive compounds, such as polyphenols, dietary fiber, proteins, lipids, vitamins, organic acids, and minerals, some of which are found in greater quantities in the discarded parts than in the parts accepted by the market. These bioactive compounds offer the potential to convert food waste into value-added products, and fields including nutritional foods, bioplastics, bioenergy, biosurfactants, biofertilizers, and single cell proteins have welcomed food waste as a novel source. This review reveals the latest insights into the various sources of food waste and the potential of utilizing bioactive compounds to convert it into value-added products, thus enhancing people’s confidence in better utilizing and managing food waste.

Developing a cuts-based system to improve consumer acceptability of pork: Impact of gender, ageing period, endpoint temperature and cooking method

Publisher: Elsevier BV

Date: 11-2016

DOI: 10.1016/J.MEATSCI.2016.06.011

Abstract: The effect of gender (entire male, female and castrate), ageing period (2 or 7days) and endpoint temperature (70 or 75°C) on consumer perceptions of cuts from the loin (Musculuslongissimus thoracis et lumborum), silverside (Musculus biceps femoris) and shoulder (Musculus triceps brachii (roast) and Musculus supraspinatus (stir fry)) when roasted or stir fried (all primals) or grilled as steaks (loin only) was investigated. Higher scores for juiciness (P=0.035), flavour (P=0.017), overall liking (P=0.018), quality grade (P=0.026) were obtained from castrates than entire males, with females intermediate. Neither ageing period nor endpoint temperature, as main effects, influenced sensory scores. Loin steaks and silverside roasts obtained lower (P<0.001) scores for all sensory traits except aroma scores for shoulder cuts were highest (P<0.001). Cooking to 70°C improved (P<0.05) juiciness, flavor and overall liking scores of loin steaks compared with 75°C. Different pathway interventions are required to optimize eating quality of different pork cuts and the cooking methods used to prepare them.

Comparison of different dietary magnesium supplements on pork quality.

Publisher: Elsevier BV

Date: 03-1999

DOI: 10.1016/S0309-1740(98)00110-7

Abstract: Forty-eight crossbred (Large White×Landrace) boars were used to compare the effect of dietary magnesium aspartate (MgAsp), magnesium sulphate (MgSO(4)) and magnesium chloride (MgCl(2)) on muscle glycogenolysis and pork quality. The pigs were fed finisher feed supplemented with either MgAsp, MgSO(4) and MgCl(2) for 5 days prior to slaughter. At the abattoir, all pigs received 15 electric shocks from an electric goad 5min prior to slaughter. Pigs fed the diet supplemented with MgSO(4) had the highest plasma Mg concentrations at slaughter in comparison with pigs fed the MgAsp and MgCl(2) supplemented diets. There were no differences in plasma adrenaline and noradrenaline concentrations at slaughter between the different diets. Pigs fed the Mg diets had higher muscle glycogen concentrations in the Longissimus thoracis (LT) muscle at 5min and at 40min (except MgCl(2)) post-slaughter compared to pigs fed the control diet. Also pigs fed the Mg diets had lower muscle lactic acid concentrations in the LT at 5min post-slaughter and lower drip loss at 24hr post-slaughter compared to pigs fed the control diet. These results indicate that cheaper magnesium sources, MgSO(4) and MgCl(2), are as efficacious as MgAsp in reducing drip loss and improving pork quality.

Antioxidant dynamics in the live animal and implications for ruminant health and product (meat/milk) quality: role of vitamin E and selenium

Publisher: CSIRO Publishing

Date: 2014

DOI: 10.1071/AN14334

Abstract: The global population is predicted to grow to over 9 billion by the middle of 21st century, with 70% of people living in urban areas, and food demand is projected to grow by 70% by 2050. Climate change presents a series of challenges for global animal agriculture. As a result of thermal challenges associated with climate variability, availability of quality pasture, animal behaviour, physiological and immunological functions are potentially impacted. Oxidative status plays an important role in the regulation and maintenance of several physiological and immunological functions of the body. Ruminants are exposed to several environmental and metabolic challenges that can trigger oxidative stress. In this scenario, it is possible for an increase in free radical production and a depletion of antioxidant reserves, resulting in damage to lipids, proteins and DNA. Since oxidative stress can affect animal health and the quality of their products (meat/milk), antioxidant supplementation of ruminant diets represents a useful tool to sustain redox homeostasis when the ruminants are exposed to oxidative stress. This paper will examine the roles that oxidative stress plays in some physiological functions, and it will discuss the implications of antioxidant supplementation on ruminant health and production. Physiological levels of dietary antioxidants underpin efficient energy utilisation, optimal antioxidant potential, and balanced mitochondrial function to enhance protein deposition without impacting animal health. The research conducted over the last decade has improved the understanding of physiological functions of antioxidants, with selenium and vitamin E receiving particular attention. There is evidence that the functions of selenium and vitamin E extend beyond the classical antioxidant properties to immunomodulation especially when administered at higher doses than recommended. Improving the oxidative status of ruminants will play an important role in delivering high-quality milk and meat products to consumers. Considering the growing awareness among consumers of the benefits of antioxidant-rich food, there is a great opportunity for the livestock industries to focus on producing antioxidant-enriched milk and meat products or functional foods. Therefore, the premise of this paper is to review the recent developments in understanding antioxidant dynamics in ruminants and their role in reducing the impact of environmental stress and metabolic diseases. In addition, the paper will explore the putative implications that antioxidant supplementation has on the quality animal products and how the improved understanding can be best utilised to achieve efficient and sustainable animal production systems to ensure quality animal products for human consumption.

Lifetime and post-weaning determinants of performance indices of pigs

Publisher: CSIRO Publishing

Date: 2003

DOI: 10.1071/AR02172

Abstract: The present study was designed to determine the interrelationships between sex, weaning age, and weaning weight on subsequent growth performance. Ninety-six Large White × Landrace pigs were used in a 2 × 2 × 2 factorial experiment with the respective factors being: age at weaning (14 or 28 days), weight at weaning (heavy or light), and sex (boar or gilt). Eighty pigs were offered a high quality pelleted weaner diet ad libitum while the remaining 16 pigs (2 pigs from each treatment group) were removed from the sow and fasted for 24 h before being euthanased for determination of gut histology and enzymology. The remaining pigs were weaned into in idual pens and given an ad libitum diet containing 15.5 MJ DE/kg and 0.95 g available lysine/MJ DE. On Day 6 and 13 after weaning, 2 pigs from each group at each time had their feed removed and, 24 h later, were euthanased. From 3 weeks post-weaning, the remaining pigs were group-penned with contemporary pigs and fed commercial rations until slaughter at 23 weeks of age. In the first week after weaning, the heavy pigs and those weaned at 28 days ate more feed and grew faster, and gilts ate more and grew faster than boars over the same time. Pigs that were heavier at weaning were also heavier at every subsequent age. At slaughter, heavy boars weighed more than heavy gilts (110.5 v. 103.7 kg, P = 0.027), whereas this was not the case for light boars and gilts (94.1 v. 94.4 kg, P = 0.96). Whereas there were no effects of sex or weight at weaning on P2 backfat depth, pigs weaned at 14 days had more backfat at 23 weeks than pigs weaned at 28 days (13.1 v. 10.9 mm, P = 0.009). In conclusion, these data clearly indicate that the greatest determinants of immediate post-weaning performance under the present conditions were the age and weight of the pigs at weaning. However, the key determinant of lifetime growth rate appeared to be weight of pigs at weaning or, by inference, birth. Although age at weaning had no effect on lifetime growth rate, early-weaned pigs were fatter at slaughter.

High dietary vitamin E and selenium improves feed intake and weight gain of finisher lambs and maintains redox homeostasis under hot conditions

Publisher: Elsevier BV

Date: 04-2016

DOI: 10.1016/J.SMALLRUMRES.2016.02.011

Genetic Selection for Thermotolerance in Ruminants

Publisher: MDPI AG

Date: 11-11-2019

DOI: 10.3390/ANI9110948

Abstract: Variations in climatic variables (temperature, humidity and solar radiation) negatively impact livestock growth, reproduction, and production. Heat stress, for instance, is a source of huge financial loss to livestock production globally. There have been significant advances in physical modifications of animal environment and nutritional interventions as tools of heat stress mitigation. Unfortunately, these are short-term solutions and may be unsustainable, costly, and not applicable to all production systems. Accordingly, there is a need for innovative, practical, and sustainable approaches to overcome the challenges posed by global warming and climate change-induced heat stress. This review highlights attempts to genetically select and breed ruminants for thermotolerance and thereby sustain production in the face of changing climates. One effective way is to incorporate sustainable heat abatement strategies in ruminant production. Improved knowledge of the physiology of ruminant acclimation to harsh environments, the opportunities and tools available for selecting and breeding thermotolerant ruminants, and the matching of animals to appropriate environments should help to minimise the effect of heat stress on sustainable animal genetic resource growth, production, and reproduction to ensure protein food security.

Bioaccessibility, Bioavailability and Bioactivities of Carotenoids in Microalgae: A Review

Publisher: Informa UK Limited

Date: 14-01-2023

DOI: 10.1080/87559129.2023.2165095

Paradoxical increases of circulating nonesterified fatty acids in somatotropin treated cattle undergoing mild disturbances

Publisher: Elsevier BV

Date: 07-1997

DOI: 10.1016/S0739-7240(97)00021-0

Abstract: Effects of various doses of bovine somatotropin (bST) on plasma concentrations of nonesterified fatty acids (NEFA) were studied in a 14-d Latin square with six Holstein heifers. Animals were given daily injections of excipient or bST at 12:00 p.m. and fed twice daily at 7:00 a.m. and 7:00 p.m. On Day 14, plasma NEFA remained low through the day except around the 7:00 p.m. feeding when they were substantially elevated. The elevation was significantly greater in bST-treated animals and corresponded to the excitement of the animals in anticipation of the evening feeding. To further investigate this phenomenon, a second experiment was conducted in which nine growing Holstein steers were fed hourly and received either daily intramuscular (i.m.) injection of excipient or bST (120 mg/kg BW) for 15 d in a crossover design. Daily profiles of NEFA were obtained under undisturbed conditions or concurrently with intensive handling. Although no elevations could be detected in any case in control animals, bST caused a substantial rise in NEFA concentration only when animals were subjected to intensive handling. This suggested that NEFA peaks noted in bST-treated heifers in the first experiment resulted from increased ability of adipose tissue to respond to adrenergic stimulation associated with the anticipation of feeding. Consistent with this hypothesis, plasma NEFA concentrations in bST-treated steers were increased to a greater extent during a challenge involving i.v. injection of epinephrine. This lification of adipose tissue response by bST must be considered when conducting intensive studies. Even the minimal excitement associated with blood s ling can confound the results regarding lipid mobilization, and this may have contributed to the notion that ST is a lipolytic hormone.

Remotely Sensed Imagery for Early Detection of Respiratory Disease in Pigs: A Pilot Study

Publisher: MDPI AG

Date: 09-03-2020

DOI: 10.3390/ANI10030451

Abstract: Respiratory diseases are a major problem in the pig industry worldwide. Due to the impact of these diseases, the early identification of infected herds is essential. Computer vision technology, using RGB (red, green and blue) and thermal infrared imagery, can assist the early detection of changes in animal physiology related to these and other diseases. This pilot study aimed to identify whether these techniques are a useful tool to detect early changes of eye and ear-base temperature, heart rate and respiration rate in pigs that were challenged with Actinobacillus pleuropneumoniae. Clinical observations and imagery were analysed, comparing data obtained from animals that showed some signs of illness with data from animals that showed no signs of ill health. Highly significant differences (p 0.05) were observed between sick and healthy pigs in heart rate, eye and ear temperature, with higher heart rate and higher temperatures in sick pigs. The largest change in temperature and heart rate remotely measured was observed around 4–6 h before signs of clinical illness were observed by the skilled technicians. These data suggest that computer vision techniques could be a useful tool to detect indicators of disease before the symptoms can be observed by stock people, assisting the early detection and control of respiratory diseases in pigs, promoting further research to study the capability and possible uses of this technology for on farm monitoring and management.

Post-weaning and whole-of-life performance of pigs is determined by live weight at weaning and the complexity of the diet fed after weaning

Publisher: Elsevier BV

Date: 12-2017

DOI: 10.1016/J.ANINU.2017.01.001

Profiling Postprandial Thermogenesis in Muscle and Fat of Sheep and the Central Effect of Leptin Administration

Publisher: The Endocrine Society

Date: 27-12-2007

DOI: 10.1210/EN.2007-1311

Abstract: Brown adipose tissue thermogenesis is an important component of energy expenditure as exemplified in rodents. Other tissues such as white adipose tissue and muscle are also capable of thermogenesis, but regulation of heat production in these tissues is poorly understood. We used a relatively large animal model, the ovariectomized sheep, in which site-specific temperature measurements were made as an index of thermogenic output. Dataloggers were implanted into the retroperitoneal (visceral) fat, gluteal (sc) fat, and skeletal muscle of the hind limb, and were programmed to record temperature every 15 min. Animals (n = 4) were then placed on a feeding schedule (fed between 1100 and 1600 h) to entrain a postprandial response in thermogenesis. Baseline thermogenesis (0800–1100 h) was higher (P & 0.05) in visceral fat and muscle than in gluteal fat, whereas the litude of the postprandial increase was similar at all three sites. Intracerebroventricular infusion into the lateral ventricle of either vehicle (artificial cerebrospinal fluid) or leptin (10 μg/h at 100 μl/h) for 24 h (0900–0900) was performed in a cross-over design with a 1-wk recovery period between treatments. Central leptin infusion did not alter the basal thermogenic rate but markedly enhanced the postprandial response in both fat and muscle tissues. This was manifest by increased (P & 0.05) litude and duration of the postprandial thermogenic response, and the effect was greater in muscle and visceral fat than in gluteal fat. These data demonstrate that leptin is able to regulate thermogenesis in muscle, providing a novel target for the manipulation of energy balance.

The Effect of Sonication on Bubble Size and Sensory Perception of Carbonated Water to Improve Quality and Consumer Acceptability

Publisher: MDPI AG

Date: 12-09-2019

DOI: 10.3390/BEVERAGES5030058

Abstract: Bubbles are important for carbonated beverage quality since smaller bubbles contribute to higher acceptability. Therefore, the effects and acceptability of the application of audible sound in carbonated water were studied using three brands and applying five frequencies for one minute each in ascending order. Six s les, two from each brand, were used for treatments: (i) control and (ii) sonication. Physicochemical measurements consisted of total dissolved solids (TDS), electric conductivity (EC), pH, bubble size, and bubble size distribution. A sensory session (N = 30) was conducted using the Bio-Sensory application to assess acceptability and emotions using self-reported and biometric responses. Statistical analysis included: ANOVA (α = 0.05) and principal component analysis (PCA) for quantitative data and Cochran Q test with pairwise comparisons (p 0.05) for self-reported emotion responses. Results showed that the sonication effect for the s le with higher TDS, EC, and pH (SPS) reduced bubble size by 46%, while in those with lowest TDS, EC, and pH (IceS) caused an increase of 158% compared to the control. For s les with intermediate values (NuS), there were non-significant differences (p 0.05) compared to the control. Acceptability was higher for s les with sonication for the three brands. Emotional self-reported responses were more positive for s les with sonication, showing significant differences (p 0.05) for emotions such as “happy” and “pleased” during both sound and visual assessments. From PCA, a positive relationship between bubble size and liking of bubbles was found as well as for the number of medium bubbles and happy facial expression. The audible sound generated by ubiquitous sound systems may potentially be used by the industry, applying it to the bottled product to modify bubble size and improve quality and acceptability of carbonated beverages.

Accuracy of dual energy X-ray absorptiometry (DXA), weight and P2 back fat to predict whole body and carcass composition in pigs within and across experiments

Publisher: Elsevier BV

Date: 12-2003

DOI: 10.1016/S0301-6226(03)00077-0

Feeding Conjugated Linoleic Acid without a Combination of Medium-Chain Fatty Acids during Late Gestation and Lactation Improves Pre-Weaning Survival Rates of Gilt and Sow Progeny

Publisher: MDPI AG

Date: 15-02-2019

DOI: 10.3390/ANI9020062

Abstract: Feeding conjugated linoleic acid (CLA) or medium-chain fatty acids (MCFA) to dams has been shown to improve progeny growth and survival, and hence may be particularly advantageous to gilt progeny. Primiparous (n = 129) and multiparous sows (n = 123 parities 3 and 4) were fed one of four diets from day 107 of gestation (107.3 ± 0.1 days) until weaning (day 27.2 ± 0.1 of lactation): (i) control diet (ii) 0.5% CLA diet (iii) 0.1% MCFA diet and (iv) equal parts of (ii) and (iii). Progeny performance data were collected and, from a subset of sows (n = 78) and their piglets (n = 144), a colostrum (day 0), milk (day 21), and piglet serum s le (day 3) were analyzed for immunoglobulin G and several selected metabolites. Liveborn pre-weaning mortality tended to be lowest (p = 0.051) in piglets from sows fed 0.5% CLA. However, sows fed the CLA diet had more (p = 0.005) stillbirths than those on the other diets. There were few effects of diet or the dam parity x diet interaction (p ≥ 0.05) on other parameters. Overall, feeding CLA or MCFA did not improve the performance of primiparous sows, multiparous sows, or their progeny.

Dairy proteins and the regulation of satiety and obesity

Publisher: CSIRO Publishing

Date: 2007

DOI: 10.1071/EA06263

Abstract: Over the past decade there has been growing scientific evidence and public acceptance of the role that dietary protein plays in regulation of satiety, feed intake and obesity-related disorders. Dietary protein appears to suppress food intake and delay the return of hunger more than fats or carbohydrates in a manner not due to energy content alone. Also, high-protein diets support the maintenance of muscle mass when subjects reduce their energy intake, ensuring primarily adipose tissue loss. Some protein sources, particularly dairy, contain specific peptides or proteins that may elicit direct effects on satiety. The major proteins present in milk include β-lactalbumin, α-lactoglobulin, immunoglobulins, bovine serum albumin, and the various caseins. In addition, processed whey contains glycomacropeptide, which stimulates pancreatic and gastrointestinal secretion of hormones involved in satiety to a greater extent than whey alone. In the context of the literature, we show that a glycomacropeptide-rich whey protein isolate decreases feed intake and weight gain to a greater extent than a soy protein isolate in obese pigs. Also, insulin sensitivity is improved in pigs consuming high-protein diets, with these effects being independent of protein source. While, high-protein diets may decrease calcium balance and bone strength, it appears that these effects are attenuated by dairy proteins and dairy sources of calcium. These findings suggest that high-protein diets, and in particular those that contain whey proteins, may reduce hunger and food intake, thereby reducing fat deposition and improving insulin sensitivity.

Perennial Ryegrass Alkaloids Increase Respiration Rate and Decrease Plasma Prolactin in Merino Sheep under Both Thermoneutral and Mild Heat Conditions

Publisher: MDPI AG

Date: 19-08-2019

DOI: 10.3390/TOXINS11080479

Abstract: A study was undertaken to determine the effects of feeding two levels of perennial ryegrass alkaloids (nil vs. moderate) under two climatic conditions. Alkaloids were fed via endophyte-infected perennial ryegrass seed and hay. Twenty-four Merino ewe weaners (six months, initial BW 32 ± 1.7 kg) were used in a study that lasted for 21 days after 14 days of adaptation. Sheep were fed either a control or alkaloid (Alk, 110 μg/kg LW ergovaline and 75 μg/kg LW lolitrem B) supplemented diet. Sheep were exposed to either constant thermoneutral (TN, 21–22 °C, 49% RH) or mildly heated (HS, 33 °C 1000–1500 h, 28% relative humidity) conditions. Dietary Alk and HS reduced dry matter intake (DMI) (p 0.001, p = 0.02, respectively) with the combination of both reducing DMI by 42%. Reductions in DMI resulted in a lower daily gain in the Alk treatment (p 0.001). Feed digestibility was reduced in the combined treatment (p = 0.03). Rectal temperature, respiration rate, and skin temperature increased in the Alk treatment. Plasma prolactin concentrations were decreased by Alk and increased by mild HS. The data indicate that production is compromised in the presence of Alk and mild HS, with this effect being exacerbated by a combination of both.

LC-MS/MS Characterization of Phenolic Metabolites and Their Antioxidant Activities from Australian Native Plants

Publisher: MDPI AG

Date: 24-10-2022

DOI: 10.3390/METABO12111016

Abstract: Polyphenols are considered vital bioactive compounds beneficial for human health. The Australian flora is enriched with polyphenols which are not fully characterized yet. Thus, the main objective of this study was to identify and characterize the Australian native sandalwood nuts, wattle seeds, lemongrass, and old man saltbush for phenolic compounds and their antioxidant activities. In this study, we tentatively identified a total of 155 phenolic compounds including 25 phenolic acids, 55 flavonoids, 22 isoflavonoids, 22 tannins, 22 lignans, 33 stilbenes, 33 coumarins and derivatives, 12 tyrosols and derivatives, and 6 phenolic terpenes. The highest total phenolic content (TPC) (15.09 ± 0.88 mg GAE/g) was quantified in lemongrass, while the lowest TPC (4.17 ± 0.33 mg GAE/g) was measured in wattle seeds. The highest total flavonoid content (TFC) and total condensed tannins (TCT) were measured in lemongrass and wattle seeds, respectively. A total of 18 phenolic metabolites were quantified/semi-quantified in this experiment. Lemongrass contains a vast number of phenolic metabolites.

Digestive physiology of pigs 2018

Publisher: Elsevier BV

Date: 2019

DOI: 10.1017/S1751731119001769

Standardized ileal digestible lysine requirements of male pigs immunized against gonadotrophin releasing factor

Publisher: Oxford University Press (OUP)

Date: 05-2016

DOI: 10.2527/JAS.2015-9622

Abstract: An experiment was conducted to determine the standardized ileal digestible (SID) Lys requirement of entire male and male pigs immunized against gonadotrophin releasing factor (GnRF immunocastrates). A total of 420 entire male and immunocastrated (IC) male pigs weighing 60.1 kg BW (SEM 0.49) were used in a 2 × 5 factorial experiment with the main effects being gender (entire males or IC males) and 5 concentrations of SID Lys:DE ratio (0.32, 0.43, 0.54, 0.64, or 0.75 g SID Lys/MJ DE). The diets were fed for 6 wk until slaughter at 107.5 kg BW (SEM 5.72). Over the entire period, IC males had a greater ADG ( < 0.001), greater ADFI ( < 0.001), and lower G:F ( < 0.001) compared with entire males. Immunocastrated males had increased plasma urea nitrogen (PUN) concentrations compared with entire males from d 10 to 42 ( < 0.001 for all days). Plasma urea nitrogen concentration also increased as Lys concentrations increased from d 3 to 42 ( < 0.001 for all days). Using the linear-plateau model, the optimal ADG for entire males was achieved at SID Lys concentrations of 0.68, 0.62, 0.54, and 0.58 g/MJ DE whereas optimal G:F was achieved at SID Lys concentrations of 0.72, 0.60, 0.54, and 0.51 g/MJ DE for the time periods d 0 to 14, d 15 to 28, d 29 to 42, and d 0 to 42, respectively. For IC males, optimal ADG was achieved at SID Lys concentrations of 0.64, 0.43, 0.38, and 0.40 g/MJ DE whereas optimal G:F was achieved at SID Lys concentrations of 0.64, 0.43, 0.36, and 0.42 g/MJ DE for the same respective time periods. Using the quadratic polynomial model, maximum ADG for entire males was achieved at SID Lys concentrations of 0.62 and 0.58 g/MJ DE whereas maximum G:F was achieved at SID Lys concentrations of 0.59 and 0.68 g/MJ DE for d 29 to 42 and d 0 to 42, respectively. For IC pigs, maximum ADG was achieved at SID Lys concentrations of 0.69, 0.54, and 0.64 g/MJ DE whereas maximum G:F was achieved at SID Lys concentrations of 0.81, 0.54, and 0.64 g/MJ DE for d 0 to 14, d 29 to 42, and d 0 to 42, respectively. A solution could not be found using the quadratic polynomial model for entire males for d 0 to 14 for both ADG and G:F and for both entire males and IC males for d 15 to 28 within the range of Lys values tested. When both the growth performance and PUN values are considered, the results suggest that IC males show a response to dietary SID Lys similar to that of entire males for 2 wk after the second immunization against GnRF. After this, IC males have a lower requirement for SID Lys than entire males.

The testis is not the major source of circulating follistatin in the ram

Publisher: Bioscientifica

Date: 04-1996

DOI: 10.1677/JOE.0.1490055

Abstract: The aims of this study were to determine the plasma concentrations of follistatin in rams and to assess if the testis contributes to circulating follistatin and if there is uptake or production of follistatin by the head in rams. Catheters were inserted in the carotid artery, jugular vein and spermatic vein of intact rams during the non-breeding season (experiment 1 n =5) and breeding season (experiment 2 n =4). In experiment 1, blood s les were collected from 5 rams every 10 min for 4 h, commencing 20–60 min after surgery. After 2 h of s ling 1 μg gonadotrophin-releasing hormone (GnRH) was injected intravenously. In experiment 2, blood s les were collected from 4 of the rams used in experiment 1 by venipuncture 30 and 15 min before surgery and every 15 min throughout surgery. Commencing 1 h after surgery, matched s les were taken from each of the vessels every 10 min for 4 h (1–4 h after surgery), then every hour for 20 h (4–24 h after surgery) and then every 10 min for 4 h (24–28 h after surgery). In both experiments, follistatin secretion was non-pulsatile and there were no significant differences between the concentrations of follistatin in any of the vessels. There was a significant ( P ·05) increase in the concentrations of follistatin in each of the vessels throughout the 4 h of 10-min s ling in both experiments. In experiment 2 plasma concentrations of follistatin in the jugular vein were significantly ( P ·05) lower before surgery than at other stages of the experiment. During the non-breeding season (experiment 1) the concentrations of follistatin in all vessels were about 2-fold higher ( P ·001) than during the breeding season (experiment 2). Concentrations of follistatin were measured in the testicular tissue of the ram, bull, monkey and rat and were found to be 13·6, 2·1, 2·5, 0·8 ng/g testis respectively. In experiment 3, blood s les were collected every 15 min for 4 h from castrated rams ( n =6) in the absence of treatment with testosterone propionate (TP) and after 7 days of treatment with a physiological dose of TP during the breeding and non-breeding seasons. There was no effect of stage of breeding season or TP on the plasma concentrations of follistatin and these concentrations in the castrated rams were similar to the concentrations in the intact rams in experiment 2. In experiment 4, the function of Leydig cells was stimulated by administration of human chorionic gonadotrophin but this had no effect on plasma concentrations of follistatin. These experiments show that the concentrations of follistatin in the plasma of rams are measurable, that the testis is not the major contributor to circulating follistatin and that there is no significant uptake or production of follistatin by the head in rams. It appears that the contribution of the testis to circulating follistatin may vary with the stage of the breeding season, being greater during the non-breeding season than the breeding season. The gonadotrophins and testosterone do not appear to have a direct effect on the secretion of follistatin in rams. The increase in concentrations of circulating follistatin during surgery and more frequent blood s ling suggest a stress-related effect on the production of follistatin. Journal of Endocrinology (1996) 149, 55–63

Optimized conditions for rapd analysis inPinus radiata

Publisher: Springer Science and Business Media LLC

Date: 07-1998

DOI: 10.1007/BF02822712

Meta-Regression Analysis of Relationships between Fibre Type and Meat Quality in Beef and Pork—Focus on Pork

Publisher: MDPI AG

Date: 31-05-2023

DOI: 10.3390/FOODS12112215

Abstract: This meta-regression analysis was conducted to identify the relationship between fibretype cross-sectional area (CSA) and frequency (%) and meat quality traits, especially tenderness (sensory and Warner-Bratzler Shear Force, WBSF). Literature searches were conducted using specific keywords which resulted in 32 peer-reviewed manuscripts that contained averages and correlation coefficients for fibre type (frequency and CSA) and quality traits of longissimus muscle for beef and pork (7 and 25 studies respectively). Correlations were analysed in meta-regression using R-Studio and linear regression was also conducted. For the combined beef and pork analysis, only pH, WBSF, and drip loss were associated with fibre type frequency and CSA (p 0.05 for all). Limiting the analysis to pork, the key results were frequency of type I fibres were associated with decreased drip loss, increased cook loss, decreased lightness (L*) and increased sensory tenderness whereas frequency of type IIb fibres were associated with increased drip loss (p 0.05 for all). In addition, the CSA of type I and IIb fibres was associated with colour traits lightness and redness (p 0.05 for all). Future research should focus on fibre type across breeds and muscles to further understand the impacts of fibre type frequency and CSA on quality.

Plant and Dairy-Based Yogurts: A Comparison of Consumer Sensory Acceptability Linked to Textural Analysis

Publisher: MDPI AG

Date: 04-02-2022

DOI: 10.3390/FOODS11030463

Abstract: Yogurt, readily available in plant and dairy-based formulations, is widely consumed and linked with health benefits. This research is aimed to understand the sensory and textural spectrum of commercially available dairy and plant-based yogurts. In a preliminary study, qualitative focus group discussions (4 groups n = 32) were used to determine perceptions of 28 dairy and plant-based yogurts, identifying positive consumer perceptions of plant-based yogurts. A smaller subset of five spoonable and one drinkable yogurts—(Reference, Soy, Coconut, Cookies, Berry, and Drinkable) was subsequently selected for rheological and structural measurements, showing wide variations in the microstructure and rheology of selected yogurt s les. A quantitative blind sensory tasting (n = 117) showed varying yogurt acceptability, with Berry being the least-liked and Cookies being the most-liked yogurt, in terms of overall liking. The multi-factor analysis confirmed that compositional and textural elements, including protein content, gel firmness, and consistency coefficient, displayed a positive relationship with overall liking. In contrast, fat, sugar, and calories were negatively correlated to the overall liking. This research showed that texture and other compositional factors are significant determinants of the consumer acceptability of yogurt products and are essential properties to consider in product development.

Comparative Study on the Effect of Phenolics and Their Antioxidant Potential of Freeze-Dried Australian Beach-Cast Seaweed Species upon Different Extraction Methodologies

Publisher: MDPI AG

Date: 22-05-2023

DOI: 10.3390/PH16050773

Abstract: Brown seaweed is rich in phenolic compounds and has established health benefits. However, the phenolics present in Australian beach-cast seaweed are still unclear. This study investigated the effect of ultrasonication and conventional methodologies using four different solvents on free and bound phenolics of freeze-dried brown seaweed species obtained from the southeast Australian shoreline. The phenolic content and their antioxidant potential were determined using in vitro assays followed by identification and characterization by LC-ESI-QTOF-MS/MS and quantified by HPLC-PDA. The Cystophora sp. displayed high total phenolic content (TPC) and phlorotannin content (FDA) when extracted using 70% ethanol (ultrasonication method). Cystophora sp., also exhibited strong antioxidant potential in various assays, such as DPPH, ABTS, and FRAP in 70% acetone through ultrasonication. TAC is highly correlated to FRAP, ABTS, and RPA (p 0.05) in both extraction methodologies. LC-ESI-QTOF-MS/MS analysis identified 94 and 104 compounds in ultrasound and conventional methodologies, respectively. HPLC-PDA quantification showed phenolic acids to be higher for s les extracted using the ultrasonication methodology. Our findings could facilitate the development of nutraceuticals, pharmaceuticals, and functional foods from beach-cast seaweed.

Effects of dietary conjugated linoleic acid on haematological and humoral responses in the grower pig

Publisher: CSIRO Publishing

Date: 2004

DOI: 10.1071/AR04002

Abstract: The effects of conjugated linoleic acid (CLA) on the levels of total serum leucocytes, granulocytes including neutrophils, basophils, and eosinophils, as well as on monocytes and leucocytes were measured in pigs selected from a clean (minimal disease) herd. Thirty pigs were fed different rates of dietary CLA (0, 1.25, 2.5, 5.0, 7.5, and 10.0 g CLA-55/kg diet) for 8 weeks. Blood s les were collected at the end of the study for assessment of haematological and humoral responses to CLA supplementation. No difference in total white blood cells including the neutrophil, monocyte, and lymphocyte counts was observed among different dietary groups. A dose-dependent reduction (P = 0.02) in eosinophil concentrations suggests that CLA exerts anti-inflammatory activities. A 2-fold increase in the level of basophils was recorded in pigs fed lower levels of CLA (1.25 and 2.5 g CLA/kg diet) but the levels decreased gradually (P = 0.05) and were below the detection limit at the highest rate (10 g/kg) of CLA supplementation. The level of IgG was reduced by over 50% in CLA-fed pigs (P 0.001), although the response was quadratic in nature (P 0.001). T-cell population analysis showed that CD4+ cells tended (P = 0.06) to be reduced linearly with increasing inclusion of CLA in the diet. Our results suggest that dietary CLA modulates haematological and humoral responses in a dose-dependent manner.

Screening and Characterization of Phenolic Compounds and Their Antioxidant Capacity in Different Fruit Peels

Publisher: MDPI AG

Date: 09-2020

DOI: 10.3390/FOODS9091206

Abstract: Fruit peels have a erse range of phytochemicals including carotenoids, vitamins, dietary fibres, and phenolic compounds, some with remarkable antioxidant properties. Nevertheless, the comprehensive screening and characterization of the complex array of phenolic compounds in different fruit peels is limited. This study aimed to determine the polyphenol content and their antioxidant potential in twenty different fruit peel s les in an ethanolic extraction, including their comprehensive characterization and quantification using the LC-MS/MS and HPLC. The obtained results showed that the mango peel exhibited the highest phenolic content for TPC (27.51 ± 0.63 mg GAE/g) and TFC (1.75 ± 0.08 mg QE/g), while the TTC (9.01 ± 0.20 mg CE/g) was slightly higher in the avocado peel than mango peel (8.99 ± 0.13 mg CE/g). In terms of antioxidant potential, the grapefruit peel had the highest radical scavenging capacities for the DPPH (9.17 ± 0.19 mg AAE/g), ABTS (10.79 ± 0.56 mg AAE/g), ferric reducing capacity in FRAP (9.22 ± 0.25 mg AA/g), and total antioxidant capacity, TAC (8.77 ± 0.34 mg AAE/g) compared to other fruit peel s les. The application of LC-ESI-QTOF-MS/MS tentatively identified and characterized a total of 176 phenolics, including phenolic acids (49), flavonoids (86), lignans (11), stilbene (5) and other polyphenols (25) in all twenty peel s les. From HPLC-PDA quantification, the mango peel s le showed significantly higher phenolic content, particularly for phenolic acids (gallic acid, 14.5 ± 0.4 mg/g) and flavonoids (quercetin, 11.9 ± 0.4 mg/g), as compared to other fruit peel s les. These results highlight the importance of fruit peels as a potential source of polyphenols. This study provides supportive information for the utilization of different phenolic rich fruit peels as ingredients in food, feed, and nutraceutical products.

Inhibition of nitric oxide release pre-slaughter increases post-mortem glycolysis and improves tenderness in ovine muscles

Publisher: Elsevier BV

Date: 10-2008

DOI: 10.1016/J.MEATSCI.2008.02.002

Abstract: The aim of this experiment was to determine the effect of inhibiting the release of nitric oxide (NO) pre-slaughter in lambs on post-slaughter muscle metabolism and meat quality. Exercise was used as a positive control as NO is known to be released in skeletal muscle during exercise. Forty Border Leicester×Merino lambs were assigned to the treatments L-NAME (NO synthase inhibitor) infusion (0mg/kg vs. 30mg/kg, 135min pre-slaughter) and exercise (none vs. 15min immediately pre-slaughter). The inhibition of NO release using L-NAME reduced Warner-Bratzler shear force (WBSF) in the longissimus thoracis et lumborum (LTL) after 3days of ageing, while the Semimembranosous (SM) was unaffected. Inhibition of NO release with L-NAME resulted in altered glucose metabolism as indicated by reduced plasma glucose pre-slaughter particularly in exercised lambs, reduced LTL and SM glycogen of non-exercised lambs post-slaughter and increased SM lactate in exercised lambs post-slaughter. In conclusion, inhibition of NO Synthase with L-NAME pre-slaughter increases post-mortem glycolysis and improves tenderness in the loin muscle.

Bioaccessibility and bioavailability changes of phenolic compounds in pumpkins (Cucurbita moschata): A review

Publisher: Elsevier BV

Date: 06-2022

DOI: 10.1016/J.FBIO.2022.101753

Assessment of the bioaccessibility of phenolics from Australian grown lettuces by in vitro simulated gastrointestinal digestion and colonic fermentation

Publisher: Elsevier BV

Date: 08-2022

DOI: 10.1016/J.FBIO.2022.101754

Using Recombinant Superoxide Dismutase to Control Oxidative Stress in the Gastrointestinal Tract of Cyclic Heat-Stressed Pigs

Publisher: MDPI AG

Date: 21-08-2023

DOI: 10.3390/ANI13162681

Abstract: Climate change is associated with an increased frequency and intensity of heat waves, posing a threat of heat stress to pig production. Heat stress compromises the efficiency of pig production partly due to causing oxidative stress, intestinal dysfunction, and inflammatory responses. Superoxide dismutase is an antioxidant enzyme reported to reduce oxidative stress and inflammation. Therefore, this experiment aimed to investigate whether recombinant superoxide dismutase (rSOD) could ameliorate oxidative stress and inflammatory responses in heat-stressed grower pigs. Sixty-four female pigs (Large White × Landrace, 27.8 ± 1.65 kg, mean ± SD) were randomly allocated to a control diet (standard grower feed, CON) or the control diet supplemented with 50 IU recombinant superoxide dismutase (rSOD) for 14 days. After acclimation to the diet, pigs were then housed under thermoneutral (TN, 20 °C, 35–50% relative humidity) or cyclic heat stress conditions (CHS, at 35 °C: 9 a.m. to 5 p.m. and 28 °C: 5 p.m. to 9 a.m., 35–50% relative humidity) for 3 days. Heat stress increased respiration rate (RR), skin and rectal temperature (RR and RT) (p 0.001 for all), and reduced plasma thyroid hormone concentration (p 0.001). The amount of oxidized glutathione (GSH:GSSG) was increased in the jejunum and ileum of CHS pigs. In the jejunum, rSOD also increased the amount of oxidized glutathione in both TN and CHS pigs, without any change in endogenous SOD activity. In the ileum, rSOD prevented increases in oxidized glutathione formation in the CHS pigs only. Taken together, this may reflect increased oxidative stress in both the jejunum and ileum in CHS pigs. Alternatively, rSOD increased the conversion of reduced to oxidized glutathione independently of CHS, possibly reflecting an increased overall SOD activity due to the addition of exogenous SOD. In conclusion, the use of in-feed SOD enzymes at a dose of 50 IU/kg may be a useful strategy for preventing oxidative stress in pigs.

Artificial Intelligence Applied to a Robotic Dairy Farm to Model Milk Productivity and Quality based on Cow Data and Daily Environmental Parameters

Publisher: MDPI AG

Date: 24-05-2020

DOI: 10.3390/S20102975

Abstract: Increased global temperatures and climatic anomalies, such as heatwaves, as a product of climate change, are impacting the heat stress levels of farm animals. These impacts could have detrimental effects on the milk quality and productivity of dairy cows. This research used four years of data from a robotic dairy farm from 36 cows with similar heat tolerance (Model 1), and all 312 cows from the farm (Model 2). These data consisted of programmed concentrate feed and weight combined with weather parameters to develop supervised machine learning fitting models to predict milk yield, fat and protein content, and actual cow concentrate feed intake. Results showed highly accurate models, which were developed for cows with a similar genetic heat tolerance (Model 1: n = 116, 456 R = 0.87 slope = 0.76) and for all cows (Model 2: n = 665, 836 R = 0.86 slope = 0.74). Furthermore, an artificial intelligence (AI) system was proposed to increase or maintain a targeted level of milk quality by reducing heat stress that could be applied to a conventional dairy farm with minimal technology addition.

Selenium and vitamin E together improve intestinal epithelial barrier function and alleviate oxidative stress in heat‐stressed pigs

Publisher: Wiley

Date: 10-05-2016

DOI: 10.1113/EP085746

Exhaled breath condensate hydrogen peroxide concentration, a novel biomarker for assessment of oxidative stress in sheep during heat stress

Publisher: CSIRO Publishing

Date: 2016

DOI: 10.1071/AN14070

Abstract: The present study aimed to measure hydrogen peroxide (H2O2) in exhaled breath condensate (EBC) as an index of oxidative stress induced by heat stress (HS) and to evaluate the antioxidant potential of supranutritional levels of selenium (Se) and vitamin E under HS conditions in sheep. Thirty-two Merino × Poll Dorset ewes (in two replicates) were acclimated to indoor in idual pen feeding of a pelleted control diet (0.24 g Se and 10 IU of vitamin E/kg DM) for 1 week. Sheep were then randomly allocated to a 2 × 2 factorial design with the respective factors being dietary Se (0.24 and 1.20 mg/kg DM as Sel-Plex) and vitamin E (10 and 100 IU/kg DM) for 2 weeks. After 1 week acclimation to the diets the sheep were placed in metabolism cages in one of two climatic chambers set to either thermoneutral (18−21°C and 40–50% relative humidity) or HS (28−40°C and 30–40% relative humidity) conditions for 1 week. Average respiration rate and rectal temperature were increased (P 0.001) during HS. Similarly, H2O2 concentration in EBC and plasma reactive oxygen metabolites were increased significantly (P = 0.041 and P = 0.002, respectively), in sheep exposed to HS. The EBC H2O2 concentration was reduced (P 0.05) in sheep fed the diet supplemented with supranutritional levels of both Se and vitamin E. Sheep supplemented with antioxidants also had lower respiration rates (191 vs 232 breaths/min, P = 0.012) and rectal temperature (40.33 vs 40.58°C, P = 0.039) under peak HS (1300 hours and 1700 hours) compared with those on the control diet indicating an improved capacity to handle HS. These data suggest that the H2O2 concentration in EBC can be utilised as a novel biomarker to demonstrate oxidative stress induced by HS in sheep.

The accuracy of dual energy X-ray absorptiometry (DXA), weight, and P2 back fat to predict half-carcass and primal-cut composition in pigs within and across research experiments

Publisher: CSIRO Publishing

Date: 2004

DOI: 10.1071/AR04052

Abstract: A Hologic QDR4500A dual energy X-ray absorptiometer (DXA) was used to measure body composition in 199 half-carcasses ranging from 15 to 48 kg. Half-carcasses were from animals of mixed sex and of either Large White × Landrace or Large White × Landrace × Duroc descent. Half-carcasses were selected from 5 different experiments to evaluate DXA accuracy within and across experiments. Values determined by DXA including total tissue mass, fat tissue mass, lean tissue mass, and bone mineral content, for the half-carcass and the shoulder, loin, belly, and ham primal cuts were evaluated by comparison with manually dissected composition. Relationships between manually dissected values and measurements of weight and backfat at the P2 site were also evaluated. Manually dissected values were strongly related to DXA-derived values, more so than with weight and P2 or a combination of both, particularly in the measurement of fat composition. In contrast to estimates derived from weight and P2, DXA-derived estimates remained accurate even when between-experiment variation was included. However, because DXA estimates were different from manually dissected values, they would need to be adjusted with the use of appropriate regression equations to correct the in-built algorithms. These results demonstrate the efficacy of DXA as a non-destructive method for determining the composition of the half-carcass and primal cuts, and its greater precision than current routinely used methods.

Basal and hormone-stimulated metabolism in lambs varies with breed and diet quality

Publisher: Elsevier BV

Date: 02-2012

DOI: 10.1016/J.DOMANIEND.2011.10.001

Abstract: The present study investigated the effects of breed and diet quality on basal and hormone-stimulated energy metabolism in lambs. Twenty-four 7-mo-old merino (MM n = 12) and second-cross (2X n = 12) lambs were maintained indoors and fed ad libitum either a low-quality (7.8% crude protein [CP] and 8.1 MJ metabolizable energy [ME]/kg dry matter [DM]) or a moderate-quality (17.6% CP and 9.1 MJ ME/kg DM) diet in a crossover design. After 3 wk of feeding, lambs were injected intravenously with insulin (10 μg/kg body weight [BW]) and epinephrine (0.8 μg/kg BW) on consecutive days and blood s les were collected at -30, -15, -1, 3, 6, 10, 15, 20, 30, 45, 60, 90, and 120 min relative to time of injection. Lambs fed the low-quality diet had lower DM (P < 0.001), CP (P < 0.01), and ME (P < 0.001) intakes than lambs fed the moderate-quality diet. Baseline nonesterified fatty acid (NEFA) concentrations were higher (P < 0.001) in lambs fed a low-quality diet than in those fed a moderate-quality diet but there were no breed differences. Second-cross lambs had higher basal plasma concentrations of glucose (P < 0.001), lactate (P < 0.001), and cortisol (P < 0.02) than the MM lambs, although there was no effect of diet on any of these plasma variables. Insulin injection caused a rapid hypoglycemic response in all lambs but the response was more pronounced (P < 0.01) in MM lambs compared with 2X lambs. The cortisol response to insulin was twice as great (P < 0.05) in MM lambs compared with 2X lambs. There was a rebound in plasma NEFA concentrations after approximately 30 min postinjection that was most pronounced (P < 0.01) in MM lambs. Epinephrine injection caused a rapid increase in plasma NEFA, which tended to be lower in lambs fed the moderate-quality diet (P = 0.07) than in those fed the low-quality diet, but did not differ between breeds. Epinephrine injection caused rapid hyperglycemia, with the response being lower (P < 0.006) in lambs fed the moderate-quality diet compared with those fed the low-quality diet and greater (P < 0.050) in 2X than in MM lambs. Epinephrine injection caused a rapid increase in plasma lactate that tended to be greater (P = 0.07) in 2X lambs compared with MM lambs. The present study demonstrated clear breed differences in basal and hormone-stimulated metabolism, such that the 2X lambs appeared to be less sensitive to insulin and more sensitive to epinephrine than the MM lambs. These metabolic differences may be related to the fundamental differences in physiology that are associated with meat and wool production from 2X and MM breeds, respectively. They may also be related to adaptation of the MM breed to harsh environments through the ability of the body to metabolize fat resources, which are an efficient source of energy for survival.

Sheep category can be classified using machine learning techniques applied to fatty acid profiles derivatised as trimethylsilyl esters

Publisher: CSIRO Publishing

Date: 2010

DOI: 10.1071/AN10034

Abstract: Eruption of permanent incisors (dentition) is used as a proxy for age for defining meat quality in Australian sheep meat. However, this approach may not be reliable. While not presently available, an objective method could be used to determine sheep age, and thus sheep category, which would then potentially remove any inaccuracies that may occur in classifying sheep meat product. Statistical classification algorithms have been successfully used in bioinformatics. In this paper we review the performance of three algorithms (support vector machines, recursive partitioning and random forests) for determining sheep age. The algorithms were applied to the measured fatty acid profiles of fat s les from 533 carcasses 254 lamb ( year old), 131 hogget (~1–2 years old) and 148 mutton ( years old) s les. Three data pretreatments (range transformation, column mean centering and range transformation with mean centering) were also examined to determine their impact on the performance of the algorithms. The random forests algorithm, when applied to mean-centred data, gave 100% predictive accuracy when classifying sheep category. This approach could be used for the development of an objective test for determining sheep age and category.

Effects of nitric oxide and oxidation in vivo and postmortem on meat tenderness

Publisher: Elsevier BV

Date: 09-2005

DOI: 10.1016/J.MEATSCI.2005.04.008

Abstract: Metabolic processes in muscle tissue in vivo result in the production of reactive oxygen species and oxidative compounds including superoxide anions and nitric oxide (NO). Reactive oxygen species can react with both lipids and proteins and often have deleterious effects, contributing to the onset of ageing and senescence as well as cell death. Nitric oxide (NO) is a free radical that is constantly produced or released throughout the body by erse tissues and is known to influence proteolytic activity in human and rodent skeletal muscle as well as being involved in regulation of calcium homeostasis in the muscle cell. The influence of nitric oxide on development of meat tenderness has been studied through postmortem manipulation and also through in vivo studies. The effect of NO on meat tenderness is postulated to be via its regulatory effects on the proteins calpain, cathepsins, ryanodine receptor channel in the sarcoplasmic reticulum (SR) and the sarcoplasmic-endoplasmic release calcium ATPase in the SR. NO is an oxidant although the effects of NO on effector proteins can be distinguished from a direct oxidation reaction. The onset of oxidation in meat postmortem is well known to produce off-odours, discolouration and unacceptable flavours associated with rancidity. Oxidation during the immediate postmortem period appears to inhibit tenderisation during ageing, probably through an inhibitory effect of oxidation on the calpain enzyme. Oxidation of muscle tissue occurring as a result of availability of oxygen during modified atmosphere packaging may also have deleterious consequences for tenderness development during storage of meat prior to retail display. In conclusion, it is proposed that postmortem meat tenderisation is influenced by skeletal muscle's release of NO pre-slaughter and the oxidation of proteases postmortem. This proposal is compatible with the existing tenderness model and will hopefully assist in increasing the accuracy of prediction of meat tenderness. Future directions for research are discussed.

Screening of Phenolic Compounds in Australian Grown Berries by LC-ESI-QTOF-MS/MS and Determination of Their Antioxidant Potential

Publisher: MDPI AG

Date: 29-12-2020

DOI: 10.3390/ANTIOX10010026

Abstract: Berries are grown worldwide with the most consumed berries being blackberries (Rubus spp.), blueberries (Vaccinium corymbosum), red raspberries (Rubus idaeus) and strawberries (Fragaria spp.). Berries are either consumed fresh, frozen, or processed into wines, juices, and jams. In recent times, researchers have focused their attention on berries due to their abundance in phenolic compounds. The current study aimed to evaluate the phenolic content and their antioxidant potential followed by characterization and quantification using LC-ESI-QTOF-MS/MS and HPLC-PDA. Blueberries were highest in TPC (2.93 ± 0.07 mg GAE/gf.w.) and TFC (70.31 ± 1.21 µg QE/gf.w.), whereas the blackberries had the highest content in TTC (11.32 ± 0.13 mg CE/gf.w.). Blueberries had the highest radical scavenging capacities for the DPPH (1.69 ± 0.09 mg AAE/gf.w.), FRAP (367.43 ± 3.09 µg AAE/gf.w.), TAC (1.47 ± 0.20 mg AAE/gf.w.) and ABTS was highest in strawberries (3.67 ± 0.14 mg AAE/gf.w.). LC-ESI-QTOF-MS/MS study identified a total of 65 compounds including 42 compounds in strawberries, 30 compounds in raspberries, 28 compounds in blueberries and 21 compounds in blackberries. The HPLC-PDA quantification observed phenolic acid (p-hydroxybenzoic) and flavonoid (quercetin-3-rhamnoside) higher in blueberries compared to other berries. Our study showed the presence of phenolic acids and provides information to be utilized as an ingredient in food, pharmaceutical and nutraceutical industries.

Seasonal and stage of lactation effects on milk fat composition in northern Victoria

Publisher: CSIRO Publishing

Date: 2013

DOI: 10.1071/AN11363

Abstract: Effect of herd nutrition, time of year and season of calving on milk fat composition and physical properties were examined on irrigated commercial dairy farms in northern Victoria that made use of split-calving and a erse range of feeding systems. Twenty-four farms were included in the study, and from each farm, morning and evening milk s les were collected from 16 cows that calved in autumn and 16 cows that calved in spring. There were no significant effects of season of calving on the concentration of fatty acids or phospholipids in milk fat, but there were interactions between season of calving and time of year (P 0.001). These differences could be attributed to changes in energy balance and body condition with stage of lactation. The phospholipids comprising mainly phosphotidylcholine (PC), phosphtidylethanolamine (PE) and sphingomyelin (SP) also varied, with PC and PE being highest in late lactation and SP lowest during peak and mid lactation for both calving groups. Milk fat colour and the concentrations of free fatty acids were more influenced by factors associated with time of year rather than stage of lactation. Milk fat colour in particular showed strong seasonal variation being distinctly lighter in summer–early autumn when compared with rest of the year. Increasing the amount of concentrates fed was associated with decreases in short-chain fatty acid concentration and increases in the solid fat content of milk fat. Variations in nutritional management practices had only small (non-significant) effects on fat composition.

A Comparative Study for Nutritional and Phytochemical Profiling of Coffea arabica (C. arabica) from Different Origins and Their Antioxidant Potential and Molecular Docking

Publisher: MDPI AG

Date: 11-08-2022

DOI: 10.3390/MOLECULES27165126

Abstract: Coffee is the most widely used beverage globally and contains many bioactive compounds, including phenolic compounds, alkaloids, triterpenes, organic acids, amino acids, hormones, and fatty acids. The main objective of this study was the comparative profiling of Australian, Colombian, Ethiopian, and Peruvian C. arabica using LC-ESI-QTOF-MS/MS. In this study, we tentatively identified 136 bioactive metabolites, including five (05) organic acids, six (06) alkaloids, three (03) amino acids (l-phenylalanine, l-tyrosine, and l-pyroglutamic acid), two (02) hormones (melatonin and serotonin), two fatty acids, one (01) furopyrans (goniothalenol), one (01) carotenoid (crocetin), three (03) terpenoids, thirty-eight (38) phenolic acids, forty-one (41) flavonoids, five (05) stilbenes, three (03) lignans and twenty-three (23) other polyphenols in C. arabica. The highest TPC value (17.74 ± 0.32 mg GAE/g) was measured in Colombian coffee while the lowest TPC value (10.24 ± 0.73 mg GAE/g) was in Peruvian coffee. Colombian coffee has a higher antioxidant potential than other studied coffee s les. A total of nineteen phenolic metabolites were mapped through LC-MS/MS. Quinic acid derivatives were quantified in higher concentrations than other metabolites. Furthermore, molecular docking predicted that chlorogenic acid is a main bioactive compound that contributes to anti-Alzheimer and anti-diabetic activities of C. arabica. The obtained results indicate that C. arabica contains a vast number of bioactive compounds which have potential health benefits. Furthermore, research could be conducted to validate the effect of these metabolites on the flavor profile of coffee beverages.

Modelling passive absorption properties of phytochemicals using physicochemical properties

Publisher: International Society for Horticultural Science (ISHS)

Date: 12-2015

DOI: 10.17660/ACTAHORTIC.2015.1106.32

Reducing the Fermentability of Wheat with a Starch Binding Agent Reduces Some of the Negative Effects of Heat Stress in Sheep

Publisher: MDPI AG

Date: 28-05-2022

DOI: 10.3390/ANI12111396

Abstract: The objective of this study was to investigate the effects of reducing the fermentability of grains on thermoregulatory responses in heat stressed (HS) lambs. To achieve this, wheat grain treated with a commercial starch binding agent, Bioprotect, is compared to maize, which has already demonstrated effects in ameliorating heat stress-induced thermoregulation responses and untreated wheat grains. An initial in vitro experiment was conducted to examine cumulative gas production from the fermentation of wheat grain with different dosages of the commercial starch binding agent, Bioprotect. Based on the in vitro results, an in vivo lamb experiment was conducted using 24 Merino lambs (1 year old 42.6 ± 3.6 kg BW). The lambs were offered one of three dietary treatments: a wheat-based diet (WD), a Bioprotect treated wheat-based diet (BD), and a maize-based diet (MD). Three successive 1-week experimental periods were conducted with lambs from all dietary groups (P1, P2, and P3). During P1, lambs were exposed to a TN environment and fed a 1.7× Maintenance feed intake (MF) level in P2, lambs were kept in a HS environment and fed a 1.7× MF level and in P3, animals were kept in a HS environment and fed a 2× MF level. The in vitro experiment revealed a reduction in cumulative gas production (p 0.05) from the Bioprotect treated wheat compared to untreated wheat s les. In the in vivo component of the study, the replacement of wheat with maize or 2% Bioprotect-treated wheat reduced the respiration rate (p 0.001) and heart rate (p ≤ 0.01) of lambs during HS. There was a reduction in the concentration of blood gas variables such as a base excess of blood (BE(b)) and extracellular fluid (BE(ecf)), bicarbonate (CHCO3−), the partial pressure of carbon dioxide (pCO2), the total concentration of carbon dioxide (ctCO2), and sodium (Na+) (p ≤ 0.001 for all) during the periods of HS compared to the thermoneutral conditions. Moreover, BD- and MD-fed lambs had a higher blood potassium concentration (K+) than the WD-fed lambs (p = 0.008). The results of the present study suggest that Bioprotect can be a viable feed treatment strategy for treating rapidly fermentable grains such as wheat to alleviate the effects of HS. Further, Bioprotect-treated wheat could be an option to replace maize in concentrate rations in jurisdictions where maize is cost-prohibitive or unavailable.

Comprehensive Profiling of Most Widely Used Spices for Their Phenolic Compounds through LC-ESI-QTOF-MS² and Their Antioxidant Potential

Publisher: MDPI AG

Date: 04-05-2021

DOI: 10.3390/ANTIOX10050721

Abstract: Spices have long been used to improve food flavor, due to their appealing fragrance and sensory attributes. Nowadays, spices-based bioactives, particularly phenolic compounds, have gained attention due to their wide range of significant effects in biological systems. The present study was conducted to characterize the 12 widely used spices (allspice, black cardamom, black cumin, black pepper, cardamom, cinnamon, clove, cumin, fennel, nutmeg, star-anise, and turmeric) for their phenolics with the liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS2), polyphenols estimation, and their antioxidant potential. Total phenolics, total flavonoids, and total tannin content and their antioxidant activities were estimated in all spices. Clove and allspice had the highest value of total polyphenol content (215.14 and 40.49 mg gallic acid equivalent (GAE) per g of s le), while clove and turmeric had the highest total flavonoids (5.59 mg quercetin equivalent (QE) per g of s le) and total tannin contents (23.58 mg catechin equivalent (CE) per g of s le), respectively. On the other hand, black cumin and black pepper had the highest phosphomolybdate activity (15.61 and 15.43 mg ascorbic acid equivalent (AAE) per g of s le), while clove was almost identified with highest free radical scavenging capacity. A positive correlation was observed among phenolic compounds and antioxidant activities. In this quest, a total of 79 phenolic compounds were tentatively characterized by using LC-ESI-QTOF-MS2 including 26 phenolic acids, 33 flavonoids, 16 other polyphenols, and 4 lignans. The high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA) quantification of phenolic compounds exhibited higher phenolic acids. These results provided us some valuable information that spices have powerful antioxidant potential that can be further used in human food and animal feed as a supplement for different health promoting applications.

Dietary antioxidants at supranutritional doses modulate skeletal muscle heat shock protein and inflammatory gene expression in sheep exposed to heat stress1,2

Publisher: Oxford University Press (OUP)

Date: 11-2014

DOI: 10.2527/JAS.2014-8047

Abstract: The objective of this study was to investigate the effects of chronic heat (thermal) stress and dietary antioxidant supplementation on the expression of heat shock proteins and inflammatory genes in the skeletal muscle of sheep. Twenty-four Merino × Poll Dorset crossbred ewes were allocated to either a control (10 IU vitamin E and 0.24 mg Se/kg DM) or high-antioxidant (VitE+Se 100 IU vitamin E and 1.20 mg Se/kg DM) diet and were exposed to 2 thermal (temperature) treatments (thermoneutral [TN]: 18°C-21°C and 26%-30% relative humidity heat stress [HS]: 28°C-40°C and 40%-50% relative humidity) for 1 wk. Physiological parameters were recorded daily, and muscle biopsies were conducted at the end of thermal treatments. Total RNA was extracted from muscle s les and reverse transcribed to cDNA for real-time PCR analysis. Respiration rates and rectal temperature were increased in response to HS (84.2 vs. 161 breaths per minute and 39.52°C vs. 40.06°C for TN and HS conditions, respectively P < 0.001). There were interactions between dietary and thermal treatments, indicating that dietary antioxidant supplementation reduced respiration rate (P = 0.097) and rectal temperature (P = 0.086) of sheep during HS but not TN conditions. Skeletal muscle heat shock transcription factor 1 (HSF1) mRNA abundance was increased by HS (1.3-fold P < 0.050) but was not changed (P = 0.77) by dietary antioxidant supplementation. The expression of skeletal muscle heat shock protein 70 (HSP70) mRNA was increased (P 0.05) of dietary antioxidant supplementation. There was, however, an interaction between thermal and dietary treatments such that dietary antioxidant supplementation ameliorated the effect of HS on NF-κB and TNF-α mRNA expression. Taken together, these results indicate that high dietary antioxidants modulate skeletal muscle expression of heat shock proteins, proinflammatory cytokine, and NF-κB transcription, which may protect against HS in sheep.

Dietary betaine supplementation has energy-sparing effects in feedlot cattle during summer, particularly in those without access to shade

Publisher: CSIRO Publishing

Date: 2014

DOI: 10.1071/AN13418

Abstract: Dietary betaine supplementation improves water retention in steers and may influence lean-tissue deposition, while also acting as an osmolyte to help regulate cellular osmotic balance. This study investigated the interactions between shade and dietary betaine on carcass characteristics, tissue enzyme activity and gene expression in 48 feedlot steers during summer. Steers were randomly allocated to a 4 × 2 factorial design with the factors being dietary betaine (0, 10, 20 or 40 g) and shade (with and without shade) for 120 days. Tissue s les were obtained at slaughter and analysed for gene expression of heat shock proteins 70 and 90 (HSP70/90) and expression of heat shock factor 1 (HSF1), and enzyme activity of fatty acid synthase (FAS) and glycerol-6-phosphate dehydrogenase (G6PDH). Carcasses were evaluated for quality. Carcass weight at slaughter was not altered by shade (P = 0.18) but tended to be increased by dietary betaine (306 v. 314 kg, P = 0.09). The P8 backfat was not altered by shade (P = 0.43) or dietary betaine (P = 0.32), although there was a within dietary betaine effect whereby P8 backfat tended to be greater in steers fed 10 g compared with 40 g betaine/day (17.4 v. 14.5 mm, P = 0.06). Muscle pH at 1 h (5.97 v. 6.03, P = 0.01) and 2 h (5.73 v. 5.80, P = 0.04) post-slaughter was higher in shaded steers, and muscle pH at 1 h post-slaughter was higher in steers fed 10 or 20 g than those fed 40 g betaine/day (6.03 v. 6.03 v. 5.95, P = 0.005). Gene expression was not altered by betaine, while adipose tissues expressed more of each gene than muscle (P 0.001). The mRNA expression of HSF1 and HSP90 was influenced by a shade × betaine interaction, although the direction of this interaction was irregular (P = 0.03 and 0.03, respectively). Adipose tissue FAS and G6PDH enzyme activity was unaffected by shade and betaine. The results of this study indicate that betaine supplementation may be a successful carcass modifier in growing feedlot steers during summer. Provision of shade during summer may reduce the rate of pH decline in the first 2 h after slaughter and reduce the risk of high rigor temperature.

Antioxidant, Alpha-Glucosidase Inhibition Activities, In silico Molecular Docking and Pharmacokinetics Study of Phenolic Compounds from Native Australian Fruits and Spices

Publisher: MDPI AG

Date: 23-01-2023

DOI: 10.3390/ANTIOX12020254

Abstract: Native Australian fruits and spices are enriched with beneficial phytochemicals, especially phenolic compounds, which are not fully elucidated. Therefore, this study aimed to analyze native Australian mountain-pepper berries (Tasmannia lanceolata), rosella (Hibiscus sabdariffa), lemon aspen (Acronychia acidula), and strawberry gum (Eucalyptus olida) for phenolic and non-phenolic metabolites and their antioxidant and alpha-glucosidase inhibition activities. Liquid chromatography–mass spectrometry–electrospray ionization coupled with quadrupole time of flight (LC-ESI-QTOF-MS/MS) was applied to elucidate the composition, identities, and quantities of bioactive phenolic metabolites in Australian native commercial fruits and spices. This study identified 143 phenolic compounds, including 31 phenolic acids, 70 flavonoids, 10 isoflavonoids, 7 tannins, 3 stilbenes, 7 lignans, 10 other compounds, and 5 limonoids. Strawberry gum was found to have the highest total phenolic content (TPC—36.57 ± 1.34 milligram gallic acid equivalent per gram (mg GAE/g), whereas lemon aspen contained the least TPC (4.40 ± 0.38 mg GAE/g). Moreover, strawberry gum and mountain pepper berries were found to have the highest antioxidant and anti-diabetic potential. In silico molecular docking and pharmacokinetics screening were also conducted to predict the potential of the most abundant phenolic compounds in these selected plants. A positive correlation was observed between phenolic contents and biological activities. This study will encourage further research to identify the nutraceutical and phytopharmaceutical potential of these native Australian fruits.

Porcine somatotropin and cysteamine hydrochloride improve growth performance and reduce back fat in finisher gilts

Publisher: CSIRO Publishing

Date: 2007

DOI: 10.1071/EA06243

Abstract: Porcine somatotropin (pST) treatment of pigs consistently improves daily gain and feed conversion ratio but the daily injections involved can be problematic. The sulfhydryl compound cysteamine hydrochloride (CSH) increases somatotropin secretion in several species, possibly through inhibition of somatostatin secretion. Therefore, it is possible that dietary CSH supplementation may provide a dietary means of increasing pST secretion and improving growth performance in finisher pigs. Eighteen female crossbred (Large white × Landrace) gilts (59.2 kg) were allocated to one of three treatments with the respective factors being a control diet, dietary CSH (0.7 g/kg) and intramuscular pST (5 mg/day) for 5 weeks. After slaughter the whole half carcass was cut into primal cuts which were then dissected to a retail level into lean, fat, bone and rind. Over the first 2 weeks of the study daily gain was increased by both pST (+46%, P 0.001) and CSH (+12%, P 0.05) while feed conversion ratio was decreased by pST (–30%, P 0.001) and CSH (–9.4%, P = 0.08) over this period. Over the entire 5 weeks there was a large and sustained increase in daily gain in pigs treated with pST (+38%, P 0.001), while dietary CSH tended to cause a more modest increase in daily gain (+7.4%, P = 0.06). As a result, final liveweight (+10.6 and +2.0 kg, respectively) and carcass weight (+5.8 and +1.6 kg, respectively) were increased by pST and CSH. Back fat was decreased by both pST and CSH (–1.7 and 1.0 mm, respectively). Total dissectible tissue was increased by pST (+9.1%, P 0.001) and CSH (+3.3%, P 0.05). In pigs treated with pST, the increased tissue was primarily as lean (+11.1%, P 0.008) and bone (+17.8%, P = 0.017), whereas an accumulation of non significant increases in lean, rind and bone was responsible for the increased dissectible tissue in the carcass of pigs supplemented with CSH. In conclusion, dietary CSH increased daily gain and carcass weight and decreased FCR and P2 backfat, with responses being most pronounced over the first 2 weeks of dietary supplementation and not as great as those caused by exogenous pST treatment.

Sa1441 Gas Sensor Capsules: A New Paradigm in Gastroenterology for Assessing Functional Roles of the Gut Microbiota

Publisher: Elsevier BV

Date: 04-2016

DOI: 10.1016/S0016-5085(16)31110-6

Insulin-like growth factor-I and analogues increase growth in artificially-reared neonatal pigs

Publisher: Cambridge University Press (CUP)

Date: 06-2002

DOI: 10.1079/BJNBJN2002574

Assessment of Feed Value of Chicory and Lucerne for Poultry, Determination of Bioaccessibility of Their Polyphenols and Their Effects on Caecal Microbiota

Publisher: MDPI AG

Date: 20-05-2022

DOI: 10.3390/FERMENTATION8050237

Abstract: Chicory and lucerne possess high feed value for poultry being good sources of protein and fiber. In addition, they are rich in polyphenols that help the body build an integrated antioxidant system to prevent damage from free radicals and positively modulate microbial populations in the gastrointestinal tract. These health-promoting effects of polyphenols depend on their bioaccessibility and absorption in the animal body. The present paper aimed to study the bioaccessibility of polyphenols from chicory and lucerne after subjecting the s les to gastric and intestinal phases of digestion in an in vitro model of chicken gut and assessment of their feed value by measuring the presence of fermentable substrates (in terms of gas production), SCFAs produced and their effects on gut microbiota population during in vitro cecal fermentation. Results revealed that the bioaccessibility of polyphenols varied with different polyphenol compounds. The highest bioaccessibility was recorded for p-hydroxybenzoic acid (90.8%) from chicory following the intestinal phase of digestion. The lowest bioaccessibility was observed for quercetin-3-rhamnoside (12.6%) from chicory after the gastric phase of digestion. From lucerne, the highest bioaccessibility was recorded for kaempferol-3-glucoside (77.5%) after the intestinal phase of digestion. Total gas production was higher for lucerne (39.9 mL/g) than chicory (28.1 mL/g). Similarly, total SCFAs production was higher after 24 h of cecal fermentation with lucerne (42.2 mmol L−1) as compared to chicory (38.1 mmol L−1). Results also revealed that the relative abundance of Clostridium was reduced with chicory (0.225%) and lucerne (0.176%) as compared to the control (0.550%) after 24 h of cecal fermentation. The relative abundance of Streptococcus was reduced by lucerne (4.845%) but was increased with chicory (17.267%) as compared to the control (5.204%) after 24 h of fermentation. These findings indicated that chicory and lucerne differentially affected the microbial populations during in vitro cecal fermentation.

Increasing the Dietary Concentration of Lupinus albus L. Decreased Feed Intake and Daily Gain of Immunocastrated Male Pigs

Publisher: MDPI AG

Date: 23-06-2021

DOI: 10.3390/ANI11071866

Abstract: An experiment was conducted to determine the appropriate dietary concentration of albus lupins that would lower feed intake and decrease backfat while optimizing the effect on the growth rate of immunocastrated male pigs. The pigs were fed albus lupins (varying from 0 to 200 g/kg) from 2 weeks after the last immunization against GnRF for 14 d prior to slaughter (where d 0 is the day of the last immunization against GnRF). Increasing the dietary albus lupin concentration decreased daily gain for d 15 to 28 (p = 0.004). Daily feed intake also decreased as the concentration of the albus lupins increased for d 15 to 28 (p 0.001). Carcass weight and backfat decreased as the concentration of dietary albus lupins increased (p = 0.011 and p = 0.024, respectively). The albus lupin concentration to maximize growth rate, minimize feed intake, maximize carcass weight and minimize backfat depth was 120, 142, 62.7 and 138 g/kg, respectively.

Impacts of Fermentation on the Phenolic Composition, Antioxidant Potential, and Volatile Compounds Profile of Commercially Roasted Coffee Beans

Publisher: MDPI AG

Date: 19-10-2023

DOI: 10.3390/FERMENTATION9100918

Basal diet and indigestible marker influence apparent digestibilities of nitrogen and amino acids of cottonseed meal and soybean meal in pigs

Publisher: Elsevier BV

Date: 09-2019

DOI: 10.1016/J.ANINU.2019.02.001

Understanding dominance: The effect of changing the definition of dominance when using TDS with consumers

Publisher: Wiley

Date: 02-05-2022

DOI: 10.1111/JOSS.12750

Abstract: Temporal dominance of sensations (TDS) is a widely used method to assess dynamic sensory perception. While TDS has been studied more extensively with trained panels, there is growing interest in testing the method with consumers. However, little is known about how consumers interpret the notion of “dominance” to decide which attribute they should select at a given point in time. This study investigated the effect of a changing definition of dominance on TDS results. Consumers, all of who had never undertaken a TDS test before, were recruited in three separate groups where they were briefly trained on TDS where dominance was defined as either: (a) most attention‐catching sensation (TDS session), (b) most intense sensation (TIS session), or (c) most changing sensation (TCS session). Results showed that TDS curves were similar between all three sessions, suggesting that consumers’ TDS results are only marginally influenced by the definition of dominance. TDS is being used increasingly by consumers, for sensory research both in academia and industry. This study demonstrates to users of the TDS method that the definition of dominance is unlikely to influence results when working with consumers. Furthermore, this study illustrates the capability of TDS using consumers for the sensory evaluation of food.

Antioxidative Properties and Phenolic Profile of the Core, Pulp and Peel of Commercialized Kiwifruit by LC-ESI-QTOF-MS/MS

Publisher: MDPI AG

Date: 08-09-2022

DOI: 10.3390/PR10091811

Abstract: The kiwifruit is cultivated globally due to its ersity of phytochemicals, especially phenolic compounds, which have antioxidant, anti-inflammatory and anti-cancer medical effects. However, only the pulp of the kiwifruit is consumed, while the peels and cores—which are also rich in phytochemicals—are usually wasted. Meanwhile, detailed information on the comparison among the three parts is still limited. In this study, the antioxidant potentials in the core, pulp, and peel of the three most commercialized kiwifruit cultivars (Australian-grown Hayward kiwifruit, New Zealand-grown Zesy002 kiwifruit, and New Zealand-grown organic Hayward kiwifruit) were selected. Their antioxidant capacities were tested, and their phenolic profiles were identified and characterized by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS). The antioxidant results showed that the peel of New Zealand-grown organic Hayward kiwifruit contained the highest total phenolic content (9.65 mg gallic acid equivalent (GAE) mg/g) and total antioxidant capacity (4.43 mg ascorbic acid equivalent (AAE) mg/g), respectively. In addition, the antioxidant capacity of the peel is generally higher than that of the pulp and cores in all species, especially ABTS (2,2-Azino-bis-3ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging ability), ranging from 13.25 mg AAE/g to 18.31 mg AAE/g. The LC-ESI-QTOF-MS/MS tentatively identified the phenolic compounds present in the three kiwifruit species, including 118 unique compounds in kiwifruit peel, 12 unique compounds in the kiwifruit cores, and three unique compounds in kiwifruit pulp. The comprehensive characterization of the phenolics in the kiwifruits’ parts indicates the importance of their waste part as a promising source of phenolics with antioxidant properties. Therefore, this study can guide the industry with meaningful information on kiwifruit waste, and can provide it with the utilization of food and pharmacological aspects.

Rosiglitazone maleate increases weight gain and body fat content in growing lambs

Publisher: CSIRO Publishing

Date: 2016

DOI: 10.1071/AN14885

Abstract: Thiazolidinediones (TZD) are synthetic orally active peroxisome proliferator-activated receptor γ ligands used in the treatment of diabetes mellitus. The peroxisome proliferator-activated receptor γ gene plays an important role in regulating fat cell development, energy balance, and lipid metabolism in adipose and skeletal muscle tissue. There is interest in pharmacologic or nutritional means that may complement genetic techniques to improve growth and carcass composition of lambs and the major aim of the present study was to determine whether TZD impact on growth performance and meat quality of growing lambs. An initial study with four cross-bred lambs confirmed that rosiglitazone maleate is absorbed after oral dosing for 7 days. A second study was conducted with 30 cross-bred lambs to investigate the effects of sex (ewe vs wether) and dose of orally administered rosiglitazone maleate (0, 8 and 24 mg/day) for 55 days on growth performance, body composition, plasma metabolites and insulin and meat quality. Feed intake tended to increase linearly with dose of TZD (1521, 1816 and 1878 g/day for 0, 8 and 24 mg/day, P = 0.07) over the entire study, and particularly during the second half of the study (P 0.05). There were both linear (P = 0.05) and quadratic (P = 0.04) responses in average daily gain to TZD (215, 270 and 261 g/day) with the quadratic response being most pronounced over the second half of the study (P = 0.004). As a result of the increased feed intake back fat (9.4, 11.1 and 13.5 mm, P 0.001) and carcass fat (27.5%, 29.2% and 30.1%, P = 0.05) increased linearly with dose of TZD. However, there was no effect of TZD on internal fat depots. Plasma non-esterified acid concentrations increased linearly (0.37, 0.39 and 0.41 mM, P = 0.01) whereas plasma insulin concentrations (23.2, 26.9 and 20.9 mU/L, P = 0.05) and the homeostatic model assessment (6.82, 7.73 and 5.98, P = 0.05) exhibited quadratic responses to TZD. There were no significant effects of TZD on muscle pH, temperature or colour although muscle pH was higher at any temperature in ewes (+ 0.05 of a pH unit, P = 0.036) than in wethers. In conclusion, these data confirm that rosiglitazone maleate was rapidly absorbed from the digestive tract of growing ruminant lambs and was metabolically active. Oral TZD treatment appeared to mitigate against the inhibitory effect of carcass fatness on feed intake but the additional energy consumed was in turn deposited as fat.

Dietary betaine reduces the negative effects of cyclic heat exposure on growth performance, blood gas status and meat quality in broiler chickens

Publisher: MDPI AG

Date: 16-05-2020

DOI: 10.3390/AGRICULTURE10050176

Abstract: Heat stress (HS) impairs growth performance and has a severe impact on lipid and protein metabolism, leading to serious adverse effects on meat quality. Forty-eight day-old-male Ross-308 chicks were assigned to two temperature conditions, thermoneutral or cyclical HS, and fed with either a control diet (CON) or the CON plus betaine (BET). Heat stress increased rectal temperature (p 0.001), respiration rate (p 0.001) and increased blood pH (p = 0.017), indicating that HS caused respiratory alkalosis. Heat stress reduced body weight during the final stage of growing period (p = 0.005), while BET improved it (p = 0.023). Heat stress tended to reduce breast muscle water content and drip loss (p = 0.089 and p = 0.082), while both were improved with BET (p = 0.008 and p = 0.001). Heat stress tended to reduce the myofibril fragmentation index (p = 0.081) whereas it increased with BET (p = 0.017). Heat stress increased thiobarbituric acid reactive substances (p = 0.017), while BET improved it (p = 0.008). Meat tenderness was not affected by HS, but was improved with BET (p 0.001). In conclusion, BET improved growth performance over the latter stages of the growing period, and improved product quality of broiler chickens when chickens exposed to HS.

Potential nutritional strategies for the amelioration or prevention of high rigor temperature in cattle - a review

Publisher: CSIRO Publishing

Date: 2014

DOI: 10.1071/AN13303

Abstract: Environmental conditions influence animal production from an animal performance perspective and at the carcass level post-slaughter. High rigor temperature occurs when the animal is hyperthermic pre-slaughter, and this leads to tougher meat. Hyperthermia can result from increased environmental temperature, exercise, stress or a combination of these factors. Consumer satisfaction with beef meat is influenced by the visual and sensory traits of the product when raw and cooked, with beef consumers commonly selecting tenderness of the product as the most important quality trait. High rigor temperature leads to a reduction in carcass and eating quality. This review examines some possible metabolic causes of hyperthermia, with focus on the importance of adipose tissue metabolism and the roles of insulin and leptin. Potential strategies for the amelioration or prevention of high rigor temperature are offered, including the use of dietary supplements such as betaine and chromium, anti-diabetic agents such as thiazolidinediones, vitamin D, and magnesium (Mg) to provide stress relief.

Growth performance and characterization of meat quality of broiler chickens supplemented with betaine and antioxidants under cyclic heat stress

Publisher: MDPI AG

Date: 22-08-2019

DOI: 10.3390/ANTIOX8090336

Abstract: Heat stress (HS) causes oxidative stress, which compromises broiler performance and meat quality. The aim of this study was to determine whether dietary antioxidants could be used as an amelioration strategy. Seventy-two day-old-male Ross-308 chicks were exposed to either thermoneutral or cyclical heat stress conditions. Diets were either control commercial diet (CON), CON plus betaine (BET), or with a combination of betaine, selenized yeast, and vitamin E (BET + AOX). Heat stress increased the rectal temperature (p 0.001), respiration rate (p 0.001), decreased blood pCO2 (p = 0.002), and increased blood pH (p = 0.02), which indicated the HS broilers had respiratory alkalosis. Final body weight was decreased by HS (p 0.001), whereas it was improved with BET (p = 0.05). Heat stress reduced cooking loss (p = 0.007) and no effect on drip loss, while BET decreased the drip loss (p = 0.01). Heat stress reduced the myofibril fragmentation index (p 0.001) and increased thiobarbituric acid reactive substances (p 0.001), while these were improved with the combination of BET + AOX (p = 0.003). In conclusion, BET overall improved growth rates and product quality in this small university study, whereas some additional benefits were provided by AOX on product quality in both TN and HS broilers.

Effect of high-protein feed supplements on concentrations of growth hormone (GH), insulin-like growth factor-I (IGF-I) and IGF-binding protein-3 in plasma and on the amounts of GH and messenger RNA fo

Publisher: Bioscientifica

Date: 09-1993

DOI: 10.1677/JOE.0.1380421

Abstract: Three groups of mature rams were maintained on diets of hay, hay+2% lupin or hay+2% cowpea for 11 weeks. Serial blood s les were taken at 15-min intervals for 12 h for the determination of GH and IGF-I content by radioimmunoassay and for IGF-binding protein-3 (IGFBP-3) levels by Western blotting. The rams were killed after 77 days of supplementary feeding and their pituitary glands analysed for content of GH and GH mRNA. Mean plasma GH and baseline GH levels were significantly ( P ·01) decreased in the rams fed lupin and cowpea compared with controls fed hay and GH pulse litude was significantly ( P ·001) decreased in the group fed the cowpea diet. The frequency of GH pulses was not significantly altered by either treatment. Plasma concentrations of IGF-I were elevated in rams fed lupin ( P ·001) or cowpea ( P ·05). IGFBP-3 levels were not significantly ( P ·05) altered by either treatment. There were no significant differences in pituitary content of GH mRNA but pituitary content of GH was increased in rams fed lupin ( P ·05) and cowpea ( P =0·07). In conclusion, a high-protein diet decreases plasma GH levels and increases IGF-I without changing plasma IGFBP-3 levels in rams. Thus ongoing synthesis of GH, as indicated by the mRNA levels, may cause a build up of GH stores in the pituitary gland. Journal of Endocrinology (1993) 138, 421–427

High dietary selenium and vitamin E supplementation ameliorates the impacts of heat load on oxidative status and acid-base balance in sheep1,2

Publisher: Oxford University Press (OUP)

Date: 07-2015

DOI: 10.2527/JAS.2014-8731

Abstract: The objective of this study was to determine the efficacy of supranutritional dietary selenium and vitamin E (Vit E) to ameliorate the effect of heat stress (HS) on oxidative status and acid-base balance in sheep. Thirty-two Merino × Poll Dorset ewes were acclimated to indoor in idual pen feeding of a pelleted control diet (0.24 g Se and 10 IU of Vit E/kg DM) for 1 wk. Sheep were then moved to metabolism cages in climatic chambers and randomly allocated to a 2 × 2 × 2 factorial design with the respective factors being dietary Se (0.24 and 1.20 mg/kg DM as Sel-Plex Alltech, Australia), Vit E (10 and 100 IU/kg DM), and temperature for 2 wk. After 1 wk of acclimation in metabolic cages, 1 climatic chamber continued on thermoneutral (TN) conditions (18°C to 21°C and 40% to 50% relative humidity [RH]), and the other one was set to HS conditions (28°C to 40°C and 30% to 40% RH) for 1 wk. The sheep were then returned to in idual pens and fed the control diet for 1 wk before being returned to the same diet as in the first period but a reversed thermal treatment for a further 2 wk. Physiological parameters were recorded 3 times daily, and blood s les were collected on d 1 and 7 of thermal treatment. Average respiration rate and rectal temperature of sheep were increased (P < 0.001) during HS however, combined supranutritional supplementation of Se and Vit E reversed the effects of HS. Sheep given the high Se and high Vit E diet had a lower respiration rate (191 vs. 232 breaths/min P = 0.012) and rectal temperature (40.33°C vs. 40.58°C P = 0.039) under peak HS (1700 h) compared with those fed the low Se and low Vit E diet. Plasma reactive oxygen metabolites concentrations were reduced (P = 0.048) by 20%, whereas biological antioxidant potential was increased (P = 0.17) by 10% in sheep fed the high Se and high Vit E diet compared with those fed the low Se and low Vit E diet. Blood pH was elevated (P = 0.007) and bicarbonate was reduced (P = 0.049) under HS, and again, these effects were ameliorated by the high Se and high Vit E diet. Both white blood cell glutathione peroxidase gene expression and red blood cell lysate glutathione peroxidase activity were increased in sheep fed the high Se and high Vit E diet. These data suggest that supranutritional dietary Se or Vit E can reduce some of the negative effects of HS. However, the synergism between the 2 antioxidants improves their potential to ameliorate the impacts of HS in sheep.

Dietary Lecithin Decreases Skeletal Muscle COL1A1 and COL3A1 Gene Expression in Finisher Gilts

Publisher: MDPI AG

Date: 07-06-2016

DOI: 10.3390/ANI6060038

Physiological Effects of Ergot Alkaloid and Indole-Diterpene Consumption on Sheep under Hot and Thermoneutral Ambient Temperature Conditions

Publisher: MDPI AG

Date: 02-06-2016

DOI: 10.3390/ANI6060037

Response of plasma glucose, insulin, and nonesterified fatty acids to intravenous glucose tolerance tests in dairy cows during a 670-day lactation

Publisher: American Dairy Science Association

Date: 2015

DOI: 10.3168/JDS.2014-8205

Abstract: This experiment investigated the metabolic response of dairy cows undergoing an extended lactation to a frequently s led intravenous glucose tolerance test. The experiment used 12 multiparous Holstein cows that calved in late winter in a seasonally calving pasture-based system and were managed for a 670-d lactation by delaying rebreeding. In each of four 5-wk experimental periods commencing at approximately 73, 217, 422, and 520 (±9.1) days in milk (DIM), cows were offered a diet of perennial ryegrass (73 and 422 DIM) or pasture hay and silage (217 and 520 DIM) supplemented with 1kg of DM grain (control CON) or 6kg of DM grain (GRN) as a ration. Daily energy intake was approximately 160 and 215 MJ of metabolizable energy/cow for the CON and GRN treatments, respectively. At all other times, cows were managed as a single herd and grazed pasture supplemented with grain to an estimated minimum daily total intake of 180 MJ of metabolizable energy/cow. Cows were fitted with an indwelling jugular catheter during the final week of each experimental period. The standard intravenous glucose tolerance test using 0.3g of glucose per kilogram of body weight was performed on each cow at approximately 100, 250, 460, and 560 DIM. Plasma concentrations of glucose, insulin, and nonesterified fatty acids (NEFA) responses were measured. Milk yield, milk solids yield, body weight, and basal plasma glucose were greater in the GRN compared with the CON treatment. The area under the plasma response curve relative to baseline (AUC) for glucose, insulin, and NEFA and their apparent fractional clearance rates indicated varied whole body responsiveness to insulin in terms of glucose metabolism throughout the 670-d lactation. The glucose AUC 0 to 20 min postinfusion was increased at 560 DIM, indicating reduced utilization of glucose by the mammary gland at this stage of lactation. The NEFA clearance rate, 6 to 30 min postinfusion, was greater at 460 and 560 DIM. These data indicated an increase in lipogenic activity or a decrease in lipolysis as lactation progressed, suggestive of an overall increase in responsiveness to insulin in terms of whole body lipid metabolism as lactation progressed. These observations are consistent with decreased priority of lactation beyond 300 DIM. Cows in the GRN treatment had decreased whole body responsiveness to hyperglycemia compared with CON cows in terms of glucose clearance and AUC for the glucose response. Variation in the response curves of plasma glucose, NEFA, and insulin was predominantly a result of stage of lactation and not diet. This may be due to changes in mammary gland uptake of glucose that is independent of insulin and the responsiveness of peripheral tissues to the actions of insulin at different stages of the lactation that are independent of diet.

Effect of growth hormone administration on IGF binding protein-3 mRNA levels in porcine tissues

Publisher: Bioscientifica

Date: 06-1999

DOI: 10.1677/JME.0.0220261

Abstract: The effect of short-term GH treatment on steady-state insulin-like growth factor binding protein-3 (IGFBP-3) mRNA levels in liver, kidney, longissimus dorsi muscle, stomach and jejunum was examined in pigs. Ten female crossbred pigs were allocated to either saline or GH (70 microg/kg/day) treatment by subcutaneous injection for 4 days. They were allowed to feed ad libitum, and were weighed daily. At the end of the treatment period, the pigs were slaughtered and s les of liver, kidney, skeletal muscle, stomach and jejunum were collected and total RNA was extracted. Steady-state levels of IGFBP-3 mRNA were quantified by RNase protection assay and were compared with the level of IGF-I class 1 and class 2 transcripts. IGFBP-3 mRNA increased in response to GH in both liver and kidney, but not in the other tissues s led. Hepatic IGF-I mRNA responded to short-term GH treatment with a fourfold increase in IGF-I class 1 mRNA and an eightfold increase in IGF-I class 2 mRNA, which was liver specific. IGF-I class 1 mRNA was not responsive to GH treatment in other tissues. The short-term nature of this treatment suggests that the increase in hepatic IGFBP-3 and IGF-I transcripts is a relatively early response to treatment with GH, and that the increase in plasma concentrations of IGFBP-3 in response to GH are derived from the liver, the kidney, or both.

Leptin-mediated effects of undernutrition or fasting on luteinizing hormone and growth hormone secretion in ovariectomized ewes depend on the duration of metabolic perturbation

Publisher: Wiley

Date: 03-2004

DOI: 10.1111/J.0953-8194.2004.01157.X

Early weaning has minimal effects on lifetime growth performance and body composition of pigs

Publisher: CSIRO Publishing

Date: 2010

DOI: 10.1071/AN09059

Abstract: Two hundred and forty pigs (120 entire boars and 120 gilts) were selected in three replicates of 40 boars and 40 gilts and housed in pens of 20 pigs of the same sex. Pigs were allocated to a 2 by 2 factorial experiment, with the respective factors being sex (entire male or female) and age at weaning (13 or 21 days). Pigs within each replicate were weaned on the same day, with the pigs’ farrowing date differing by ~8 days for the two weaning ages. Pigs were offered ad libitum access to feed for the entire experimental period. Eight randomly selected pigs from each pen were tagged as focus animals. These animals underwent dual energy X-ray absorptiometry (DXA) scanning six times from weaning through to slaughter to measure changes in body composition. The animals weaned at 13 days of age were lighter at weaning (4.68 ± 1.16 and 6.84 ± 1.34 kg, respectively, for the animals weaned at 13 and 21 days, P 0.001). During the first 4 days after weaning pigs weaned at 21 days of age consumed more feed (72.1 v. 30.9 g/day, P 0.001) and gained faster (35.0 v. –63.0 g/day, P = 0.042) than those weaned at 13 days. The pigs weaned at 13 days did, however, ‘catch up’ to be the same weight as those weaned at 21 days by 53 days of age (17.4 and 17.8 kg, respectively, P = 0.33). Daily gain from birth to 146 days of age did not differ across treatments (610, 597, 640, 657 g/day, respectively, for the gilts weaned at 13 days, gilts weaned at 21 days, boars weaned at 13 days and boars weaned at 21 days, P = 0.31). DXA analyses indicated that the animals weaned at 13 days had a greater percentage of lean tissue at 119 days of age (78.4 and 76.8%, respectively, P = 0.039) although this was not maintained through to slaughter. There were no treatment effects on the percentage of adipose tissue from 90 to 146 days of age, although the DXA estimated adipose tissue mass was greater at 146 days of age in the animals weaned at 21 days (13.8 and 15.2 kg, respectively, for the animals weaned at 13 and 21 days of age, P = 0.023, s.e.d. 0.60). These data suggest that weaning age predominately influences growth immediately after weaning, and does not have a major influence on lifetime growth performance or body composition at commercial slaughter weights.

Hyperinsulinaemia, supplemental protein and branched-chain amino acids when combined can increase milk protein yield in lactating sows

Publisher: Cambridge University Press (CUP)

Date: 03-2005

DOI: 10.1079/BJN20041366

Abstract: The aim of this study was to determine whether dietary supplementation with branched-chain amino acids, and the infusion of insulin and dextrose, would increase milk protein secretion in the sow. The experiment involved sixteen lactating sows fed either a normal lactation diet (162 g/kg crude protein, n 8) or a high-protein diet (230 g/kg crude protein, n 8) supplemented with branched-chain amino acids (valine, isoleucine and leucine). Sows were either infused with insulin and dextrose or not infused at all during mid (day 5–10) and late (day 17–22) lactation in a single reversal design. Blood s les were analysed for glucose, and the dextrose infusion rate was adjusted to maintain the blood glucose level within 15 % of pre-infusion levels. Milk (10·1 v. 11·1 kg/d P =0·014) and\\ lactose (628 v. 727 g/d P =0·002) yield increased with insulin infusion, whereas milk protein content (5·0 % v. 5·5 % P =0·007) was increased in diets supplemented with protein and branched-chain amino acids. Piglet growth was increased by feeding the higher-protein diet (237 v. 273 g/d P =0·05) but not significantly increased by insulin infusion (245 v. 265 g/d P =0·11). These effects were additive such that the combined treatment resulted in a 24 % (56 g/d P ·05) increase in piglet growth rate. These data demonstrate that increasing the dietary protein/branched-chain amino acid content can increase milk protein secretion but not milk yield. The infusion of insulin and dextrose increased milk and milk lactose yields, and tended to increase milk protein yield but not milk protein content. These effects are additive and translate to increased protein yield and piglet growth.

The impact of pre-slaughter stress on beef eating quality

Publisher: MDPI AG

Date: 27-08-2019

DOI: 10.3390/ANI9090612

Abstract: The study evaluated the relationship between pre-slaughter stress, plasma biomarkers and consumer-evaluated eating quality of pasture raised beef cattle (n = 488). The design tested steer only, heifer only and mixed sex cattle with a comparison of direct kill versus a 14 day rest period in abattoir holding paddocks prior to slaughter. Experiment One sourced cattle from four farms and tested shipping and road transport. Experiment Two sourced cattle from four farms and tested a commercial saleyard pathway. The impact on treatment on untrained consumer eating quality scores were tested on five muscle groups, m. psoas major, m. longissimus dorsi lumborum, m. biceps femoris, m. semitendinosis, and m. infraspinatus. Across all muscles, a two-week rest period had the biggest improvement in sensory score. Mixed groups scored lower in the outside muscle than non-mixed groups. However, the mixing response was inconsistent in the eye round muscle and not significant in the other muscles. Plasma glucose and L-lactate indicated a marked acute stress response at slaughter with a small detrimental impact on consumer score. The muscle damage enzyme markers creatine kinase (CK) and aspartate aminotransferase (AST) were strongly associated with a lower meat quality score (MQ4). Neither β-hydroxybutyrate (βHB) nor non-esterified fatty acids (NEFA) were associated with MQ4, suggesting that fat mobilisation does not impact consumer sensory score.

A two-pool model of tritiated water kinetics to predict body composition in unfasted lactating goats

Publisher: Cambridge University Press (CUP)

Date: 12-1988

DOI: 10.1017/S0003356100003585

Abstract: A two-pool model of tritiated water kinetics was investigated as a means of partitioning total body water into empty body water and gut water in 17 lactating goats. Empty body water, gut water and total body water were of a similar magnitude to, and highly correlated with, a rapidly equilibrating tritiated water pool, a more slowly equilibrating pool and the sum of these two pools, respectively. Empty body fat was poorly correlated with both live weight and empty body weight ( R 2 = 0·42 and 0·51, respectively). However, there was a strong inverse relationship between the water and fat contents of the empty body. Consequently, empty body fat was accurately predicted by a multiple regression equation which included both empty body weight and empty body water as independent variables ( R 2 = 0·97). Substitution of these variables with estimates derived from tritiated water kinetics still resulted in a high correlation ( R 2 = 0·88). Tritiated water kinetics offered little improvement over live weight alone in the prediction of empty body protein, empty body ash or fat-free empty body.

Muscle vitamin E and retail colour of meat from lambs fed lucerne or grain-based diets at two antioxidant levels.

Publisher: Elsevier BV

Date: 06-2016

DOI: 10.1016/J.JNIM.2015.12.206

Muscle Antioxidant Enzymes Activity and Gene Expression Are Altered by Diet-Induced Increase in Muscle Essential Fatty Acid (α-linolenic acid) Concentration in Sheep Used as a Model

Publisher: MDPI AG

Date: 28-03-2019

DOI: 10.3390/NU11040723

Abstract: This study investigated the effect of dietary manipulations on muscle fatty acid composition, the activities and relative mRNA expressions of antioxidant enzymes and the relationship between muscle enzyme activity or mRNA expression and alpha linolenic acid (ALA) concentration in sheep. Eighty-four lambs blocked on liveweight were randomly allocated to four dietary treatments, lucerne pasture (Lucerne), annual ryegrass pasture (Ryegrass), feedlot pellets (Feedlot) or annual ryegrass plus feedlot pellets (RyeFeedlot). After six weeks of feeding, lambs were slaughtered and within 30 min post-mortem, s les collected from the longissimus lumborum (LL) muscle for RNA isolation and measurement of antioxidant enzyme activities. At 24 h post-mortem, LL s les were collected for determination of fatty acid concentrations. Feedlot treatment decreased ALA, eicosapentaenoic (EPA), docosapentaenoic (DPA) and docosahexaenoic (DHA) concentrations compared with other treatments and increased linoleic acid (LA) and arachidonic acid (AA) compared with Lucerne and Ryegrass (p 0.001). The activity of Glutathione peroxidase (GPX1, p 0.001) and Superoxide dismutase (SOD2, p 0.001) enzymes in the muscle increased with Lucerne compared to other treatments. Lucerne increased muscle gpx1 mRNA expression by 1.74-fold (p = 0.01) and 1.68-fold (p = 0.05) compared with Feedlot and other diets, respectively. The GPX1 (r2 = 0.319, p = 0.002) and SOD2 (r2 = 0.244, p = 0.009) enzyme activities were positively related to ALA. There was a positive linear relationship between muscle gpx1 (r2 = 0.102, p = 0.017) or sod2 (r2 = 0.049, p = 0.09) mRNA expressions and ALA concentration. This study demonstrates that diet can affect concentrations of ALA and other fatty acids as well as change activities and gene expression of antioxidant enzymes in muscle. Increased antioxidant activity may, in turn, have beneficial effects on the performance, health and wellbeing of animals and humans.

Cinnamon: A Natural Feed Additive for Poultry Health and Production—A Review

Publisher: MDPI AG

Date: 07-07-2021

DOI: 10.3390/ANI11072026

Abstract: The increased bacterial resistance to synthetic antibiotics and consumer awareness about the health and food safety concerns have triggered the ban on the use of antibiotic growth promotors (AGPs) in the poultry industry. This situation encouraged the poultry sector and industry to explore safe alternatives to AGPs and focus on developing more sustainable feed management strategies to improve the intestinal health and growth performance of poultry. Consequently, phytogenic feed additives (PFAs) have emerged as natural alternatives to AGPs and have great potential in the poultry industry. In recent years, cinnamon (one of the most widely used spices) has attracted attention from researchers as a natural product with numerous health benefits for poultry. The essential oils in cinnamon, in particular, are of interest because of their antioxidant, anti-microbial, anti-inflammatory, antifungal, and hypocholesterolaemic effects, in addition to their ability to stimulate digestive enzymes in the gut. This review mainly emphasizes the potential impact of cinnamon as a natural feed additive on overall gut health, nutrient digestibility, blood biochemical profile, gene expression, gut microbiota and immune response.

Dietary Lipids Influence Bioaccessibility of Polyphenols from Black Carrots and Affect Microbial Diversity under Simulated Gastrointestinal Digestion

Publisher: MDPI AG

Date: 17-08-2020

DOI: 10.3390/ANTIOX9080762

Abstract: The bioaccessibility and activity of polyphenols is dependent on their structure and entrapment in the food matrix. While dietary lipids are known to transit into the colon, the impact of different lipids on the microbiome, and their interactions with dietary polyphenols are largely unknown. Here, we investigated the effect of dietary lipids on the bioaccessibility of polyphenols from purple/black carrots and adaptation of the gut microbiome in a simulated in vitro digestion-fermentation. Coconut oil, sunflower oil, and beef tallow were selected to represent common dietary sources of medium-chain fatty acids (MCFAs), long-chain polyunsaturated fatty acids (PUFAs), and long-chain polysaturated fatty acids (SFAs), respectively. All lipids promoted the bioaccessibility of both anthocyanins and phenolic acids during intestinal digestion with coconut oil exhibiting the greatest protection of anthocyanins. Similar trends were shown in antioxidant assays (2,2-Diphenyl-1-pricrylhydrazyl (DPPH), ferric reducing ability (FRAP), and total phenolic content (TPC)) with higher phytochemical bioactivities observed with the addition of dietary lipids. Most bioactive polyphenols were decomposed during colonic fermentation. Black carrot modulated ersity and composition of a simulated gut microbiome. Dramatic shifts in gut microbiome were caused by coconut oil. Inclusion of sunflower oil improved the production of butyrate, potentially due to the presence of PUFAs. The results show that the impact of polyphenols in the digestive tract should be considered in the context of other components of the diet, particularly lipids.

Immunisation against gonadotrophin-releasing hormone (GnRH) increases growth and reduces variability in group-housed boars

Publisher: CSIRO Publishing

Date: 2011

DOI: 10.1071/AN11021

Abstract: It is generally accepted that entire boars are leaner and more efficient than barrows but that they have poorer meat quality with an increased risk of boar taint. An additional issue in producing boars to meet market specifications is variation in growth performance, which may be exacerbated by high levels of aggression and mounting behaviour in group-housed animals. Immunisation against gonadotrophin-releasing hormone (GnRH), leading to immunological castration, offers a means of reducing aggression and mounting behaviour while maintaining most of the production efficiencies associated with entire boars. A total of 120 control boars, 120 immunised boars and 60 barrows (physically castrated at 5 days of age) were used in a study conducted in two replicates. Each replicate consisted of two pens each of boars, immunised boars and barrows, each pen containing 15 pigs, and two blocks of 15 in idually-housed boars and immunised boars. The group-housed pigs had access to two single place electronic feeders per pen. Immunised boars were given the anti-GnRH vaccine (Improvac) at 14 and 18 weeks of age. In the 2 weeks immediately after the secondary immunisation at 18 weeks, average daily gain was greater in the immunised boars than the boars or barrows, particularly in those that were group housed, as indicated by the interaction (P = 0.035). From 18 to 23 weeks of age average daily gain was greater (P = 0.005) in immunised boars than in control boars and barrows and was greater (P 0.001) in in idually- than in group-housed pigs. The average daily gain of group-housed immunised boars and in idually-housed control boars were similar over the 5-week period after secondary immunisation (1090 vs 1099 g/day), being ~20 and 15% higher than the group-housed control boars and barrows, respectively. The average daily feed intake was greater (P = 0.011) in immunised boars than in control boars with barrows intermediate. The standard deviation of bodyweight increased with age but was lower (P from 0.032 to 0.09) in immunised boars than in control boars and barrows between 18 and 22 and 17 and 21 weeks of age. Carcass fighting damage and pork pH were higher (P 0.05) for control boars than for immunised boars or barrows. In conclusion, immunisation against GnRH increased average daily gain and average daily feed intake while decreasing variation in bodyweight and improving carcass and pork quality.

Impact of processing and storage on protein digestibility and bioavailability of legumes

Publisher: Informa UK Limited

Date: 21-02-2023

DOI: 10.1080/87559129.2022.2039690

Dietary Betaine Impacts Metabolic Responses to Moderate Heat Exposure in Sheep

Publisher: MDPI AG

Date: 19-05-2023

DOI: 10.3390/ANI13101691

Abstract: Dietary betaine supplementation can ameliorate physiological responses to heat exposure (HE) in sheep. This experiment measured metabolic responses to glucose (intravenous glucose tolerance, IVGTT), insulin (insulin tolerance test, ITT), and adrenocorticotropic hormone (ACTH) challenges in Merino ewes (n = 36, 39.7 kg) maintained at thermoneutral (TN, 21 °C) or HE (18–43 °C) and supplemented with either 0, 2, or 4 g/day dietary betaine (n = 6 per group). Sheep had ad libitum access to water and were pair-fed such that the intake of the TN sheep mimicked that of the HE sheep. After 21 days of treatment, sheep were fitted with jugular catheters and subjected to consecutive daily challenges (IVGTT, ITT, and ACTH, d 21–23, respectively), followed by skeletal muscle and subcutaneous adipose tissue biopsy collections for gene expression analysis (d 24). The HE-treated sheep had a greater insulin:glucose ratio (p = 0.033), a greater estimated homeostatic model assessment of insulin resistance (HOMAIR p = 0.029), and a reduced revised quantitative insulin sensitivity check index (RQUICKI p = 0.015). Sheep fed betaine (2 + 4 g/day) had a greater basal plasma insulin (p = 0.017) and a reduced basal non-esterified fatty acid (NEFA p = 0.036) concentration, while the RQUICKI was reduced (p = 0.001) in sheep fed betaine. The results suggested that betaine supplementation alters lipid metabolism by potentially improving insulin signaling, although these responses differ between TN and HE conditions. There was no other impact of temperature or dietary treatments on the tissue gene expressions measured. Our results support the notion that betaine, in part, acts to modify lipid metabolism.

Mesenteric infusion of a volatile fatty acid prevents body weight loss and transiently restores luteinising hormone pulse frequency in ovariectomised, food-restricted ewes

Publisher: Wiley

Date: 12-07-2011

DOI: 10.1111/J.1365-2826.2011.02173.X

Abstract: Pulsatile luteinising hormone (LH) secretion is suppressed by food restriction and rapidly restored by return to ad lib. feeding concomitant with an increase in the oxidation of free fatty acids, although there is no increase in plasma leptin concentrations or body fat content in ovariectomised ewes. The ingestion of food may stimulate LH secretion by increasing availability of oxidisable metabolic substrates. Ruminal digestion is characterised by the production of volatile fatty acids and, of these, propionate is the major gluconeogenic substrate, and both glucose and propionate are oxidisable in a variety of tissues. To examine whether increases in mesenteric propionate concentrations are sufficient for restoration of pulsatile LH secretion during a period of food restriction, adult, food-restricted, hypogonadotrophic, ovariectomised ewes received mesenteric vein infusions of 5 μmol/min/kg body weight (BW) propionate or saline, whereas normal weight, ad lib.-fed ewes received mesenteric infusions of saline for 10 days. Blood s les were taken every 10 min for 5 h before the start of the 10-day infusion period, and continued throughout the first 5 h of infusion on the afternoon of day 1, and in the morning on days 2, 7 and 10. Propionate-infused, food-restricted and ad lib.-fed, saline-infused ewes showed a significantly higher LH pulse frequency compared to that of food-restricted-saline-infused ewes on postinfusion days 1 and 2 but not on days 7 and 10, and only the saline-infused, food-restricted group lost a significant amount of body weight. These results indicate that the reproductive system can respond acutely to infusion of metabolic fuels such as propionate, although a sustained recovery of pulsatile LH secretion requires more than an increase in this single metabolic substrate.

Nutritional strategies affect carcass and pork quality but have no effect on intramuscular fat content of pork

Publisher: CSIRO Publishing

Date: 2012

DOI: 10.1071/AN11138

Abstract: Fifty crossbred (Large White × Landrace × Duroc) female finisher pigs were used to determine the effect of nutritional strategies on intramuscular fat content. The dietary treatments were (A) Control: commercial grower and finisher diet, (Day 68–166), (B) –15% P : E and –vitamin A: a 15% reduced protein : energy grower diet with no supplemental vitamin A (Day 68–110), followed by a commercial finisher diet (Day 111–166), (C) sugar: a grower diet supplemented with 10% sugar (Day 68–110), followed by a commercial finisher diet (Day 111–166), (D) zinc: a grower diet supplemented with 250 ppm zinc (Day 68–110), followed by a commercial finisher diet (Day 111–166), and (E) lecithin: a diet supplemented with 3 g/kg lecithin in the grower and finisher diet (Day 68–166). The effects of lecithin supplementation on compression characteristics of the M. semitendinosus were also studied. These data indicate that there were no significant effects of dietary manipulations on intramuscular fat content. During the grower phase (Day 68–110) pigs offered the low protein : energy and vitamin A-deficient diet had a poorer feed : gain compared with those offered diet containing supplemental sugar. Dietary lecithin supplementation decreased (P 0.05) hardness and chewiness values for the M. semitendinosus compared with pigs offered the Control diet. Pigs offered the lecithin-supplemented diet also tended (P = 0.090) to have lower cook loss compared with pigs offered the Control diet. Dietary zinc supplementation during the grower phase improved (P 0.05) the carcass dressing % compared with pigs offered the other diets. Dietary sugar or zinc increased (P 0.05) the amount of lean in the belly and may be a means to control the rapid rise in the ratio of fat to lean in the belly during the finisher phase. These data indicate that dietary lecithin supplementation has the potential to improve the tenderness of pork but that intramuscular fat is difficult to manipulate nutritionally from an already moderate amount.

The energy value of Lupinus angustifolius and Lupinus albus for growing pigs

Publisher: Elsevier BV

Date: 2000

DOI: 10.1016/S0377-8401(99)00115-7

Comparative Assessment of Thermotolerance in Dorper and Second-Cross (Poll Dorset/Merino × Border Leicester) Lambs

Publisher: MDPI AG

Date: 20-12-2020

DOI: 10.3390/ANI10122441

Abstract: The objective of this study was to compare the thermotolerance of second-cross (SC Poll Dorset × Merino × Border Leicester) and Dorper lambs. Dorper and SC lambs (4–5 months of age) were subjected to cyclic heat stress (HS) (28–40 °C). The temperature was increased to 38–40 °C between 800 and 1700 h daily and maintained at 28 °C for the remainder of the day (30–60% relative humidity (RH)) in climatic chambers for 2 weeks (n = 12/group), with controls maintained in a thermoneutral (TN) (18–21 °C, 40–50% RH) environment (n = 12/group). Basal respiration rate (RR), rectal temperature (RT) and skin temperature (ST) were higher (p 0.01) in SC lambs than in Dorpers. HS increased RR, RT and ST (p 0.01) in both genotypes, but the levels reached during HS were lower (p 0.01) in Dorpers. HS increased (p 0.01) water intake to a greater extent in SC lambs, while feed intake was reduced (p 0.05) by HS in SC lambs but not in Dorpers. HS increased (p 0.01) blood urea nitrogen and creatinine in SC lambs only. Plasma non-esterified fatty acid concentrations were reduced (p 0.05) by HS in SC lambs but increased (p 0.05) in Dorpers. There was no effect of HS on pO2, cHCO3− and cSO2, but higher (p 0.01) blood pH and lower (p 0.01) pCO2 were recorded under HS in both genotypes. Blood electrolytes and base excess were reduced (p 0.01) under HS, while a genotype difference (p 0.05) was only observed in blood K+ and hemoglobin concentrations. Basal plasma prolactin concentrations were lower (p 0.01) in Dorpers but were elevated at a similar level during HS (p 0.01) in both genotypes. Dorper lambs are more resilient to HS than SC lambs. Future research should focus on confirming whether the better heat tolerance of Dorpers is translated to better returns in terms of growth performance and carcass traits over the summer months.

Seasonal variation in milk production and cheese yield from commercial dairy farms located in northern Victoria is associated with pasture and grazing management and supplementary feeding practices

Publisher: CSIRO Publishing

Date: 2007

DOI: 10.1071/EA04168

Abstract: A study of irrigated pasture-based commercial dairy farms that made use of split calving (two distinct periods of calving autumn and spring) was undertaken between April 2001 and March 2002 in northern Victoria, to examine associations between herd nutrition, time of year and season of calving and the production and composition of milk. On average, herds that had access to higher digestibility pasture or were fed more cereal grain-based concentrates produced more milk. However, the average marginal yield of 4% fat corrected milk/kg cereal grain-based concentrates was less than responses achieved under experimental conditions in northern Victoria. Herds that calved in autumn had different production characteristics to those that calved in spring, in that they did not show an early lactation peak in milk yield and produced milk with lower average concentrations of crude protein, casein and fat. Despite this, herds that calved in autumn had greater persistency of milk yield in mid to late lactation, when they tended to be better fed on pasture, so that yields of milk solids over a notional 310-day lactation were similar for both calving groups (523 v. 529 kg fat + protein autumn v. spring, respectively), but herds that calved in autumn produced milk with a lower potential to yield cheddar cheese (10.2 v. 10.6 kg cheese/100 kg milk P 0.01). Farms that produced milk in the lowest quartile for potential to yield cheddar cheese differed from the top quartile in that they: (i) milked fewer cows (175 v. 250) (ii) fed less supplements (5.6 v. 9.4 kg DM/cow.day) (iii) walked their herds shorter distances between pasture and the dairy (2.2 v. 3.2 km/day) (iv) allocated lower herbage allowances (33 v. 43 kg DM/cow.day) and (v) grazed pastures at a mass low enough to have restricted pasture intake ( 3 t DM/ha), about twice the frequency of farms (0.40 v. 0.17) in the top quartile. Greater productivity of the dairy industry in northern Victoria could be achieved through better grazing and pasture management and supplementary feeding practices on farms.

The Synergism of Biochemical Components Controlling Lipid Oxidation in Lamb Muscle

Publisher: Wiley

Date: 06-06-2014

DOI: 10.1007/S11745-014-3916-5

Abstract: Lipid oxidation of M. longissimus lumborum in fresh or vacuum packaged (aged) lamb meat stored at 3 °C for 0 or 4 weeks, respectively and displayed under refrigerated conditions for a further 4 days was assessed by measuring the concentration of malondialdehyde (MDA) in meat using the thiobarbituric acid reactive substances procedure. The effects of vitamin E, heme iron and polyunsaturated fatty acids (n-6 and n-3) on lipid oxidation were examined. Results showed a strong positive relationship between heme iron, n-6 and n-3 fatty acids and lipid oxidation when vitamin E was below 2.95 mg/kg muscle. When lipid oxidation was related to vitamin E concentration and the other three variables, respectively, any increase in heme iron or n-6 or n-3 fatty acids concentration did not influence lipid oxidation. Management of diet to elevate muscle vitamin E concentration above 3.45 mg/kg muscle is beneficial to maintain the level of lipid oxidation below 2.4 mg MDA/kg muscle in meat stored for up to 4 weeks. This demonstrates that vitamin E concentration in muscle has a greater influence on controlling lipid oxidation in muscle tissues than do heme iron or polyunsaturated fatty acids.

Invited review: An evaluation of the likely effects of individualized feeding of concentrate supplements to pasture-based dairy cows

Publisher: American Dairy Science Association

Date: 03-2015

DOI: 10.3168/JDS.2014-8475

Abstract: In pasture-based dairy systems, supplementary feeds are used to increase dry matter intake and milk production. Historically, supplementation involved the provision of the same amount of feed (usually a grain-based concentrate feed) to each cow in the herd during milking (i.e., flat-rate feeding). The increasing availability of computerized feeding and milk monitoring technology in milking parlors, however, has led to increased interest in the potential benefits of feeding in idual cows (i.e., in idualized or differential feeding) different amounts and types of supplements according to one or more parameters (e.g., breeding value for milk yield, current milk yield, days in milk, body condition score, reproduction status, parity). In this review, we consider the likely benefits of in idualized supplementary feeding strategies for pasture-based dairy cows fed supplements in the bail during milking. A unique feature of our review compared with earlier publications is the focus on in idualized feeding strategies under practical grazing management. Previous reviews focused primarily on research undertaken in situations where cows were offered ad libitum forage, whereas we consider the likely benefits of in idualized supplementary feeding strategies under rotational grazing management, wherein pasture is often restricted to all or part of a herd. The review provides compelling evidence that between-cow differences in response to concentrate supplements support the concept of in idualized supplementary feeding.

Application of small angle X-ray scattering synchrotron technology for measuring ovine meat quality

Publisher: Elsevier BV

Date: 07-2016

DOI: 10.1016/J.MEATSCI.2016.03.005

Abstract: A small angle X-ray scattering (SAXS) synchrotron was used to evaluate 100 ovine m. longissimus lumborum, representing lamb (n=50) and sheep (n=50). The diffraction of X-rays gives information on muscle myofibril structure and fat content. The linear relationships between SAXS measures with measures such as, shear force, intramuscular fat content (IMF) and collagen content/solubility, were investigated. A relationship was found between the d-spacing of the actin/myosin fibril spacing (SAX1 and SAX2) and the cross sectional area of the rhombohedral unit cell (Cell area) and shear force after 1 and 5day ageing. There was a positive relationship between IMF and a SAXS Fat area measure. There was a muscle site effect on SAX1, SAX2 and Cell area, with the cranial site having a larger distance between myofibrils. The potential of SAXS as a powerful research tool to determine not only the structural components of ovine tenderness, but also the fat content related to IMF is evident.

Exploring Meal and Snacking Behaviour of Older Adults in Australia and China

Publisher: MDPI AG

Date: 03-04-2020

DOI: 10.3390/FOODS9040426

Abstract: Sensory perception and food preferences change as we age. This paper encompassed two studies with the aim being to investigate meal and snacking behaviour of older adults towards food, especially meat products, and understand the desirable characteristics of those products. A qualitative multivariate analysis (QMA) focus group with Australian and Chinese older (60–81 years old) adults was conducted. A conjoint concept database was used to determine older consumers’ wants and needs for food in Australia and China. The QMA suggested that Australian consumers are not eating a proper breakfast or dinner but are ‘snacking’ throughout the day. In contrast, Chinese consumers are eating three regular meals through the day and occasionally snacks. For both groups, texture and flavour were key drivers for food choice. Difficulty in eating meat products was evident, e.g., beef jerky was found too dry and hard. Older consumers in China and Australia differed in responses to the four food categories investigated in terms of product traits and segmentation. Both the conjoint analysis and QMA showed that demographics have an impact on consumer preferences towards food. This research suggested that there is an opportunity to create ready-to-eat, nutrient dense products to enhance the wellness of older consumers.

Towards Sustainable Livestock Production: Estimation of Methane Emissions and Dietary Interventions for Mitigation

Publisher: MDPI AG

Date: 28-05-2021

DOI: 10.3390/SU13116081

Abstract: The increasing need for sustainable livestock production demands more research in the field of greenhouse gas (GHG), particularly methane (CH4), measurement and mitigation. Dietary interventions, management, and biotechnological strategies to reduce the environmental impacts and economic implications of enteric CH4 emissions are needed. While the use of biotechnological interventions and management strategies can be challenging on a routine basis, feed additive supplementation appears to be the most researched, developed, and ready to use strategy to mitigate enteric CH4 emissions. This paper discusses various recently developed feeding strategies to reduce enteric CH4 emissions in livestock. Additionally, the manuscript reviews various technologies developed for CH4 estimation since the accurate and reliable estimation of CH4 emissions can be a limiting step in the development and adoption of any mitigation strategy.

Milk production and body composition of single-bearing East Friesian×Romney and Border Leicester×Merino ewes

Publisher: Elsevier BV

Date: 10-2015

DOI: 10.1016/J.SMALLRUMRES.2015.08.006

Influence of housing type and age in female pigs. 1. Effects on growth performance and fat deposition and distribution in the carcasses of female Large White × Landrace pigs grown from 5.5 to 120 kg l

Publisher: CSIRO Publishing

Date: 2011

DOI: 10.1071/AN10180

Abstract: One hundred and sixty female Large White × Landrace pigs were obtained at 3 weeks of age, average liveweight (LW) 5.5 ± 0.08 kg, stratified on LW and allocated to four treatments in a factorial design that consisted of two housing treatments, conventional (C) or deep-litter (D), across two growth periods: early (3–13 weeks of age) and late (13–24 weeks of age). At 13 weeks of age eight pigs per treatment (n = 32) were slaughtered, and the remaining pigs (n = 128) moved to new pens where they remained until slaughter at 24 weeks of age. Moving pigs into a new housing system caused a growth reduction, as indicated by significantly lower LW (P = 0.003), compared with pigs that remained within the same housing system, regardless of whether the new system was C or D. Carcass composition results indicated that pigs finished in the D system (24 weeks of age) were not fatter than pigs raised in C housing, with pigs raised entirely in C housing tending to be the fattest (P = 0.090). There was an effect of housing on fat distribution within the carcass where pigs finished in D housing had significantly less fat in the belly primal compared with pigs finished in the C facilities (35.3 versus 31.2%, P = 0.030). These findings suggest that the strategy of moving pigs from D housing to C housing for finishing, to reduce carcass fatness and improve pig growth performance, was not successful as pigs were fatter, lighter and less efficient than pigs of the same age housed in D from wean to finish.

Phytochemical and Safety Evaluations of Finger Lime, Mountain Pepper, and Tamarind in Zebrafish Embryos

Publisher: MDPI AG

Date: 28-06-2022

DOI: 10.3390/ANTIOX11071280

Abstract: Plants play a pivotal role in drug discovery, constituting 50% of modern pharmacopeia. Many human diseases, including age-related degenerative diseases, converge onto common cellular oxidative stress pathways. This provides an opportunity to develop broad treatments to treat a wide range of diseases in the ageing population. Here, we characterize and assess the toxicological effects of finger lime (Citrus australasica), mountain pepper (Tasmannia lanceolata), and small-leaved tamarind (Diploglottis australis) extracts. The characterization demonstrates that these Australian native plants have antioxidant potential and, importantly, they have high concentrations of distinct combinations of different antioxidant classes. Using zebrafish larvae as a high-throughput pre-clinical in vivo toxicology screening model, our experiment effectively discriminates which of these extracts (and at what exposure levels) are suitable for development towards future therapies. The LC50-96h for finger lime and tamarind were mg/L, and 1.70 mg/L for mountain pepper. Critically, this work shows that adverse effects are not correlated to the properties of these antioxidants, thus highlighting the need for combining characterization and in vivo screening to identify the most promising plant extracts for further development. Thus, we present a high-throughput pre-clinical screening that robustly tests natural plant products to utilize the ersity of antioxidant compounds for drug development.

Compensatory feeding during early gestation for sows with a high weight loss after a summer lactation increased piglet birth weight but reduced litter size

Publisher: Oxford University Press (OUP)

Date: 03-08-2021

DOI: 10.1093/JAS/SKAB228

Abstract: Sows mated in summer produce a greater proportion of born-light piglets (& .1 kg) which contributes to increased carcass fatness in the progeny population. The reasons for the low birth weight of these piglets remain unclear, and there have been few successful mitigation strategies identified. We hypothesized that: 1) the low birth weight of progeny born to sows mated in summer may be associated with weight loss during the previous summer lactation and 2) increasing early gestation feed allowance for the sows with high lactational weight loss in summer can help weight recovery and improve progeny birth weight. Sows were classified as having either low (av. 1%) or high (av. 7%) lactational weight loss in their summer lactation. All the sows with low lactational weight loss (LLStd) and half of the sows with high lactational weight loss received a standard gestation feeding regime (HLStd) (2.6 kg/d day 0–30 gestation), whereas the rest of the sows with high lactational weight loss received a compensatory feed allowance (HLComp) (3.5 kg/d day 0–30 gestation). A comparison of LLStd (n = 75) versus HLStd sows (n = 78) showed that this magnitude of weight loss over summer lactation did not affect the average piglet or litter birth weight, but such results may be influenced by the higher litter size (P = 0.030) observed in LLStd sows. A comparison of HLStd versus HLComp (n = 81) sows showed that the compensatory feeding increased (P = 0.021) weight gain of gestating sows by 6 kg, increased (P = 0.009) average piglet birth weight by 0.12 kg, tended to reduce (P = 0.054) the percentage of born-light piglets from 23.5% to 17.1% but reduced the litter size by 1.4 (P = 0.014). A subgroup of progeny stratified as born-light (0.8–1.1 kg) or -normal (1.3–1.7 kg) from each sow treatment were monitored for growth performance from weaning until 100 kg weight. The growth performance and carcass backfat of progeny were not affected by sow treatments. Born-light progeny had lower feed intake, lower growth rate, higher G:F, and higher carcass backfat than born-normal progeny (all P & 0.05). In summary, compensatory feeding from day 0 to 30 gestation in the sows with high weight loss during summer lactation reduced the percentage of born-light progeny at the cost of a lower litter size, which should improve growth rate and carcass leanness in the progeny population born to sows with high lactational weight loss.

Influence of housing type and age in female pigs. 2. Effects on biochemical indicators of fat metabolism and the fatty acid profile of belly fat and back fat depots

Publisher: CSIRO Publishing

Date: 2011

DOI: 10.1071/AN10181

Abstract: Weaning pigs into deep-litter (D) housing systems and then moving them into conventional (C) housing facilities affects the growth paths of the pigs and can result in differences in carcass composition which may be explained by altered fat metabolism. To examine this proposition experimentally, 160 female Large White × Landrace pigs were obtained at 3 weeks of age, average liveweight 5.5 ± 0.08 kg and were stratified by weight to four treatments. The treatments consisted of two housing treatments, C or D, across two growth periods: (i) early (3–13 weeks of age) and (ii) late (13–24 weeks of age). At ~13 weeks of age eight pigs per experimental treatment (n = 32) were slaughtered and the remaining pigs (n = 128) moved to new pens where they were housed until slaughter at ~24 weeks of age. To 13 weeks of age, the effect of housing type on lipogenesis did not reach significance (P 0.05). At 24 weeks of age there were some treatment differences in fatty acid profile (P ≤ 0.05) and the concentration of plasma glycerol (P = 0.002) and non-esterified fatty acids (P = 0.019). There were trends for lipogenic enzyme activity to differ between treatments also (P 0.100). Results suggested fat deposition was lower in D-finished pigs compared with C-finished pigs, rejecting the hypothesis that D-finished pigs would be fatter. However, most of the differences in the biochemical measurements were explained by the significant reduction in growth that occurred when pigs changed housing environments, rather than as an effect of the housing environment itself. Indicators of lipogenesis suggested that lipogenic rate was lowest in pigs moved from C to D housing compared with other treatment groups that had remained within the same housing, C or D, throughout the experiment or had moved from D housing to C housing at 13 weeks of age.

The use of different models for the estimation of residual feed intake (RFI) as a measure of feed efficiency in meat sheep.

Publisher: Elsevier BV

Date: 05-2008

DOI: 10.1016/J.ANIFEEDSCI.2007.05.013

In vivo actions of IGF analogues with poor affinities for IGFBPs: Metabolic and growth effects in pigs of different ages and GH responsiveness

Publisher: Elsevier BV

Date: 1995

DOI: 10.1016/0955-2235(95)00007-0

Abstract: IGF-I analogues that bind poorly to IGFBPs are substantially more potent than IGF-I at stimulating growth in rats. However, rodents differ from other mammals because they contain only minimal circulating levels of IGF-II and they are poorly responsive to GH. In this report we review a series of experiments carried out in pigs, a species that is both GH responsive and has high blood concentrations of IGF-II. Intravenous bolus administration of IGFs to 55 kg pigs depressed blood glucose with the potency greatest for analogues such as des (1-3) IGF-I, R3IGF-I and Long R3IGF-I that showed the weakest binding to pig IGFBP-3, a similar efficacy pattern to that reported in the rat. Chronic subcutaneous administration of Long R3IGF-I, however, reduced growth rates, led to a depression in food intake and lowered concentrations of IGF-I, IGF-II and IGFBP-3. IGF-I itself depressed IGF-II concentrations and did not stimulate growth. Subcutaneous infusion of IGFs over a 3-day period, also in 55 kg pigs, demonstrated that analogues that bound least well to IGFBP-3 were the most effective at reducing the concentration of this binding protein, suggesting that the inhibition of growth was related to the depression of IGFBP-3. On the other hand, IGF-I and Long R3IGF-I increased growth rats in neonatal pigs, especially under conditions of reduced food intake. As these anabolic effects occur at a developmental stage where the animals are insensitive to GH in a manner analogous to the situation in rats, it is plausible that the feed-back inhibition of GH secretion explains the catabolic response to IGFs in older pigs.

Dietary betaine improves intestinal barrier function and ameliorates the impact of heat stress in multiple vital organs as measured by evans blue dye in broiler chickens

Publisher: MDPI AG

Date: 23-12-2020

DOI: 10.3390/ANI10010038

Abstract: In a 2 × 2 factorial design, 60 male Ross-308 broilers were fed either a control or 1 g/kg betaine diet and housed under thermoneutral (TN) or heat stress (HS) conditions. Broilers were acclimated to diets for 1 week under TN (25 °C), then either kept at TN or HS, where the temperature increased 8 h/day at 33 °C and 16 h/day at 25 °C for up to 10 days. Respiration rate (RR) was measured at four time points, and on each of 1, 2, 3, 7 and 10 days of HS, 12 broilers were injected with 0.5 mg/kg of Evans Blue Dye (EBD) solution to quantify regional changes in tissue damage. Betaine was quantified in tissues, and ileal damage was assessed via morphometry and transepithelial resistance (TER). Heat stress elevated RR (p 0.001) and resulted in reduced villous height (p = 0.009) and TER (p 0.001), while dietary betaine lowered RR during HS (p 0.001), increased betaine distribution into tissues, and improved ileal villous height (p 0.001) and TER (p = 0.006). Heat stress increased EBD in the muscle and kidney of chickens fed the control diet but not in those receiving betaine. Overall, these data indicate that supplemented betaine is distributed to vital organs and the gastrointestinal tract, where it is associated with improved tolerance of HS. Furthermore, EBD markers help reveal the effects of HS on organs dysfunction.

The influence of dietary energy intake on growth performance and tissue deposition in pigs between 80 and 120 kg liveweight

Publisher: CSIRO Publishing

Date: 2004

DOI: 10.1071/AR04041

Abstract: Eighty crossbred pigs of a composite genotype were allocated at 80 kg liveweight to a 2 × 5 factorial experiment involving 2 sexes (boars and gilts) and 5 levels of dietary energy intake ranging from about 55% estimated ad libitum up to 100% ad libitum intake. The diet was formulated to be protein-adequate and contained 14.4 MJ DE/kg and 0.55 g available lysine/MJ DE and the pigs were slaughtered at approximately 120 kg liveweight. Growth rate and food conversion efficiency increased linearly, in response to increasing digestible energy (DE) intake, with boars consistently out-performing gilts at each level of DE intake. Protein deposition rate in the whole empty body of pigs was consistently higher in boars than in gilts and linearly related to DE intake in both sexes, with no evidence of a plateau at high energy intakes, suggesting no intrinsic limit to protein deposition in these pigs up to 120 kg liveweight. When pigs were offered the protein-adequate diet ad libitum between 80 and 120 kg liveweight, boars and gilts consumed 47.7 and 40.9 MJ DE/day, respectively, and protein deposition rates in the whole empty body of pigs reached 247 and 182 g/day, respectively. Maintenance energy requirements were estimated to be 351 kJ DE/kg0.75.day for both boars and gilts [maximal model including nominal DE level (treated as a 5-level factor), actual DE intake (treated as a continuous variable), and sex (treated as a 2-level factor)] or 506 and 566 kJ DE/kg0.75.day for boars and gilts, respectively [reduced model including actual DE intake (treated as a continuous variable) and sex (treated as a 2-level factor)]. Statistical analyses show that the maximal model reflects the data more closely than the minimal model, suggesting that the lower maintenance estimate of 351 kJ DE/kg0.75.day could reflect reality better. In conclusion, genetically improved boars, and to a lesser extent gilts, have a high capacity for lean growth, and their upper limit to protein retention might not be reached below about 120 kg liveweight.

Selenium-Enriched Agaricus bisporus Mushroom Protects against Increase in Gut Permeability ex vivo and Up-Regulates Glutathione Peroxidase 1 and 2 in Hyperthermally-Induced Oxidative Stress in Rats

Publisher: MDPI AG

Date: 24-06-2014

DOI: 10.3390/NU6062478

The effects of post-weaning progestagen treatment (Regumate) of early-weaned primiparous sows on subsequent reproductive performance

Publisher: Elsevier BV

Date: 06-1998

DOI: 10.1016/S0378-4320(98)00088-8

Abstract: This study investigated the effects of feeding the orally active progestagen, altrenogest (Regumate) post-weaning on the subsequent reproductive performance of early weaned sows. Ninety (90) Large White/Landrace first parity sows were randomly assigned to three treatments. Treatment 1 (EW) and treatment 3 (CW) sows were weaned on day 12 and day 24 post-partum, respectively while treatment 2 sows (EW-R) were weaned on day 12 post-partum and received an in idual daily dose of 20 mg of Regumate on days 13 to 24 post-partum inclusive. Each sow was mated naturally at least twice at the first post-weaning or post-treatment oestrus and slaughtered on days 25-28 of pregnancy to determine the number of corpora lutea and embryos. Regumate-to-oestrus and weaning-to-oestrus intervals were similar for EW-R and CW sows (6.2 vs. 5.6 days). However, both intervals were significantly shorter (P < 0.01) than the weaning-to-oestrus interval of EW sows (7.3 days). An excellent synchronization of oestrus was achieved with Regumate treatment with 97% of treated sows in oestrus within 7 days of Regumate withdrawal compared with 64% for EW sows (P 0.05). Treatment with Regumate resulted in a significant increase in ovulation rate (16.9 vs. 15.4 and 14.9 for treatments EW-R, EW and CW, respectively P 0.05). These results indicate that Regumate feeding is a potential management tool to alleviate the diminished reproductive performance associated with early weaning regimes since it leads to successful control of oestrus, higher ovulation and embryo survival rates and thus a greater potential litter size.

Changes in insulin, glucose and ketone bodies, but not leptin or body fat content precede restoration of luteinising hormone secretion in ewes

Publisher: Wiley

Date: 11-05-2007

DOI: 10.1111/J.1365-2826.2007.01551.X

Abstract: The reproductive system, including pulsatile luteinising hormone (LH) secretion, is inhibited by deficits in energy availability and restored by energy surfeits. Plasma LH, insulin, leptin, ghrelin, glucose, ketone body, and nonesterified fatty acid concentrations were measured in ovariectomised, food-restricted ewes before and after return to ad libitum feeding to determine the factors that change in time to account for the restoration of pulsatile LH secretion. At 07.00 h, blood was s led every 10 min for 5 h from ovariectomised, hypogonadotrophic, chronically food-restricted and ad libitum-fed ewes (Fed). At 12.00 h, four of the food-restricted sheep were given ad libitum access to food (Re-Fed), while three ewes continued to be food restricted (Restricted). S ling continued for 5 h and resumed again on the mornings of days 2, 4, and 9. A pulse of LH was seen within 1 h of re-feeding in all Re-Fed ewes, and interpulse interval (IPI) was significantly shorter in Re-Fed compared to Restricted ewes and longer than in Fed ewes during the period after re-feeding. Re-Fed LH IPI was not restored to that of Fed ewes until sometime between days 4 and 9. The first pulse occurred within minutes, whereas restoration of IPI occurred after 4-8 days. Prior to the initial LH pulses seen in Re-Fed ewes, plasma ketone bodies first fell and then rose to levels significantly above those in Restricted ewes. Significant changes in circulating insulin, ghrelin, glucose, and total ketone body concentrations, daily food intake and lean body mass preceded restoration of Re-Fed LH IPI some time between days 4 and 9, but there were no significant changes in adiposity or circulating leptin concentrations, consistent with the hypothesis that LH pulses are reinitiated by changes in the availability of oxidisable metabolic fuels and possibly insulin, but not leptin concentrations.

Factors influencing the incidence of high rigor temperature in beef carcasses in Australia

Publisher: CSIRO Publishing

Date: 2014

DOI: 10.1071/AN13455

Abstract: Beef carcasses undergoing rapid pH fall while the loin muscle temperature is still high are described as heat-shortened, heat-toughened or ‘high rigor temperature’ carcasses, with subsequent negative effects on quality traits. The aim of the study was to quantify the occurrence of high rigor temperature in beef carcasses across Australia and to identify the causative factors. Data was collected over 4–5 days at each of seven beef processing plants from 1512 beef carcasses. The beef carcasses were from both grass- and grain-fed cattle ranging in days on grain feeding from 0 (grass-fed) to 350 days and the category of cattle ranged from veal to ox and cow. Data collected on the day of slaughter included the duration of electrical inputs at the immobiliser, electrical stimulation and hide puller, longissimus muscle pH and temperature decline, hot carcass weight and P8 fat depth. At grading, ultimate pH, eye muscle area, wetness of the loin surface and colour score were also collected. The temperature at pH 6 was calculated and if it was °C, the carcass was defined as ‘high rigor temperature’. Modelling of the data was conducted using GLMM and REML. The occurrence of high rigor temperature across all seven beef processing plants was 74.6% ranging from 56 to 94% between beef processing plants. Increasing days in the feedlot and heavier carcass weights were highly correlated and both caused an increase in the predicted temperature at pH 6 and in the % high rigor temperature (P 0.05 for both). Longer duration of electrical inputs at the hide puller, fatter grass-fed cattle and fatter male (castrate) carcasses had a higher temperature at pH 6 and higher % high rigor temperature. Modelling showed that if the time to reach pH 6 in the longissimus muscle was 65 v. 105 min, the % high rigor temperature carcasses reduced from 98 to 19% in grain-fed cattle and 93 to 7% in grass-fed cattle. Higher plasma insulin levels at slaughter were associated with a higher temperature at pH 6 (rigor temperature) (P 0.001). In conclusion, in order to reduce the incidence of high rigor temperature in grain-fed beef carcasses, methods for identifying high rigor temperature carcasses will be required and while some management strategies can be implemented now, others require further research.

Feed efficiency and body composition are related to cortisol response to adrenocorticotropin hormone and insulin-induced hypoglycemia in rams

Publisher: Elsevier BV

Date: 08-2010

DOI: 10.1016/J.DOMANIEND.2010.03.003

Abstract: Metabolic rate and energy consumption increase through the activation of the hypothalamic-pituitary-adrenal axis when an animal is exposed to a stressor. Residual feed intake (RFI) as a measure of efficiency has been shown to be related to exogenous adrenocorticotropin hormone (ACTH)-stimulated cortisol concentrations, which is indicative of the relationship between an animal's response to stress and the efficiency with which the energy is used for growth and production. In this study, we tested the hypothesis that sheep with low post-ACTH serum cortisol concentration relative to the other sheep in the flock have lower RFI values and lower cortisol concentrations following insulin-induced hypoglycemia. Adrenocorticotropin hormone (2.0 microg/kg body weight)-stimulated cortisol concentrations were measured in 100 sheep. The extreme responders were selected (n = 12 high cortisol, n = 12 low cortisol), and feed efficiency and body composition parameters were measured. A second ACTH challenge and an insulin challenge were administered. More efficient sheep (more negative RFI value) were found to have lower (P < 0.05) cortisol concentrations following both an ACTH challenge and an insulin challenge. Low-cortisol sheep (low response to ACTH or insulin) were found to have a lower (P < 0.05) proportion of fat tissue in comparison to the high-cortisol animals. These data clearly indicate that an animal's response to exogenous ACTH or insulin-induced hypoglycemia as a stressor is related (P < 0.05) to efficiency of energy use when measured as RFI. These data have important implications in enabling identification of animals that are superior in terms of feed efficiency and for understanding the physiological mechanisms underlying efficiency of energy use.

Comprehensive Metabolite Fingerprinting of Australian Black and Green Olives and Their Antioxidant and Pharmacokinetics Properties

Publisher: MDPI AG

Date: 13-06-2023

DOI: 10.3390/SEPARATIONS10060354

Abstract: Polyphenols, especially flavonoids, are well-known for their bioactive antioxidant properties. Therefore, this study aimed to analyze Australian black (ripe) and green olives (unripe) for phenolic and non-phenolic metabolites, antioxidant activities, and pharmacokinetic properties. Liquid chromatography–mass spectrometry coupled with quadrupole–time of flight (LC–ESI–QTOF–MS/MS) was applied to elucidate the composition, identification, and characterization of bioactive metabolites from Australian olives. This study identified 110 metabolites, including phenolic acids, flavonoids, stilbenes, lignans, and other compounds (phenolic terpenes, tyrosols, fatty acids, and terpenoids). Luteolin (flavonoid) and verbascoside (hydroxycinnamic acid) are identified with higher concentrations in black olives. Black (ripe) olives were measured at a higher TPC (10.94 ± 0.42 mg GAE/g) and total antioxidant potential than green olives. The pharmacokinetic properties (absorption, distribution, metabolism, excretion, toxicity) of phenolic compounds for human health were evaluated to predict the potential of the most abundant metabolites in olives. Gastrointestinal absorption and Caco-2 cell permeability of metabolites in olives were also predicted. This study will develop into further research to identify the Australian olives’ therapeutic, nutraceutical, and phytopharmaceutical potential.

Age and nutrition influence the concentrations of three branched chain fatty acids in sheep fat from Australian abattoirs

Publisher: Elsevier BV

Date: 11-2010

DOI: 10.1016/J.MEATSCI.2010.04.009

Abstract: The characteristic mutton odour, associated with the cooked meat of older sheep, can be problematic for some consumers who find the odour disagreeable. Branch chain fatty acids (BCFAs) are considered to be the main determinants of mutton odour. In this study, the aim was to identify the factors influencing the BCFA content of animals at abattoirs in Australia. S les of subcutaneous fat from over the chump (gluteus medius) were collected from 533 sheep carcasses at abattoirs in New South Wales, Victoria and Western Australia. The carcasses were from sheep differing in age, gender, breed and nutrition. The concentrations of three branched chain fatty acids (BCFAs) namely, 4-methyloctanoic (MOA), 4-ethyloctanoic (EOA) and 4-methylnonanoic acids (MNA), were determined. Statistical modelling showed that, with pre-slaughter nutrition in the model as a random term, BCFA concentrations could be used for discriminating the age of sheep. Fat s les from lamb carcasses had lower MOA and EOA concentrations and a higher concentration of MNA in comparison to hogget and mutton (P<0.05). When nutrition was excluded as a random effect from the statistical model, the MOA and MNA concentrations did not differentiate between lamb, hogget and mutton whereas, for EOA, lamb had a lower concentration than mutton (P<0.05) with hogget intermediate. An interaction existed between age and gender (P<0.05) where female lambs had lower EOA concentrations relative to the mutton but not for castrates.

The pattern of fat and lean muscle tissue deposition differs in the different pork primal cuts of female pigs during the finisher growth phase

Publisher: Elsevier BV

Date: 12-2004

DOI: 10.1016/J.LIVPRODSCI.2004.04.005

The effect of Lupinus albus on growth performance, body composition and satiety hormones of male pigs immunized against gonadotrophin releasing factor

Publisher: MDPI AG

Date: 02-03-2017

DOI: 10.3390/ANI7030015

Ractopamine supplementation increases lean deposition in entire and immunocastrated male pigs

Publisher: CSIRO Publishing

Date: 2009

DOI: 10.1071/AN09076

Abstract: Sixty entire male pigs (Large White × Landrace × Duroc crossbred) were in idually reared from 45 to 114 kg liveweight in a 2 by 3 factorial experiment to determine the interactive effects of sex (entire male pigs v. male pigs immunologically castrated using Improvac with vaccinations administered at 13 weeks of age and 5 weeks before slaughter) and a ractopamine feeding program (constantly fed 0 or 5 ppm ractopamine for 26 days before slaughter v. a step-up program where 5 ppm of ractopamine was fed for 14 days followed by 10 ppm ractopamine for the final 12 days before slaughter) on growth performance, carcass composition and pork quality. Following the second vaccination, immunocastrated pigs ate more (P 0.001) and grew faster (P 0.05) than entire male pigs without affecting feed conversion efficiency. Dietary ractopamine supplementation for the last 26 days before slaughter improved feed conversion ratio (P = 0.024) and daily gain (P = 0.046). Dietary ractopamine supplementation also increased carcass total tissue (P = 0.023) and total lean (P = 0.027) content without affecting the objective meat quality (P 0.05) in both entire and immunocastrated male pigs. The effects of dietary ractopamine and immunocastration were additive, such that pigs that were immunocastrated and received ractopamine grew 18% faster than control entire males. However, a step-up program of ractopamine supplementation did not provide further improvements in growth performance and carcass composition when compared with constant 5 ppm ractopamine supplementation. These findings indicate that ractopamine supplementation improved growth performance in entire and immunocastrated male pigs, thereby offering a means of improving growth performance of entire males without detrimental effects on pork quality.

Replacing starch with fat in the diet is more effective at enhancing overall performance in finisher than grower pigs

Publisher: Cambridge University Press (CUP)

Date: 24-03-2015

DOI: 10.1017/S0021859614001257

Abstract: The addition of supplementary fat to pig diets above current dietary recommendations has generally been viewed in a negative manner because it is considered that the surplus energy will be deposited into adipose tissue, and may therefore be detrimental to carcass value. The current study sought to investigate the effects of adding supplementary fat, in place of starch, to increase the energy density of male and female pig diets on growth performance and carcass quality. A total of 288 Large White × Landrace male and female pigs (boars and gilts) were randomly allocated to pens of 12 pigs each. The effects of (i) sex (gilts v. boars), (ii) added dietary fat level (as tallow 0 v. 40 and 80 g/kg) and (iii) within added dietary fat level (40 v. 80 g fat/kg), were tested for a 5-week period (Period 1) ( n = 4). These diets were formulated to contain 13·5, 14·3 and 15·2 MJ digestible energy/kg for 0, 40 and 80 g added fat/kg, respectively the calculated standardized ileal digestible lysine content was kept constant at 8·8 g/kg of diet. Thereafter the experiment was designed to test for the same effects over a second 5-week period (Period 2), during which pigs from the initial three treatments were offered the diets with 0 and 40 g added fat/kg. Treatment effects were also assessed over the whole 10 weeks of the study. There was a positive linear effect of dietary fat supplementation in Period 1 on average daily weight gain, whereas dietary fat level in Period 1 had no effect on performance in Period 2, overall, or on carcass weight and P2 backfat thickness (i.e. thickness of fat at 65 mm down the left side from the midline, at the level of the head of the last rib) at 10 weeks. In contrast, supplementation of the diet with 40 g fat/kg during Period 2, regardless of the diet offered in Period 1, increased ADG in the second period and over the entire experiment. There were minimal effects of supplementary fat on feed intake in the entire experiment however feed conversion ratio (FCR) was influenced by sex over the entire study and tended to be reduced by dietary fat supplementation in Period 2. The diet with 40 g added fat/kg fed in Period 2 increased carcass weight by an average of 2·8 kg and P2 backfat thickness by 1·2 mm. Collectively, the data from the current study suggest that dietary fat supplementation is more effective in finisher pigs than in grower pigs in terms of growth rate, FCR and carcass weight.

Selenium-enriched Agaricus bisporus increases expression and activity of glutathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon

Publisher: Elsevier BV

Date: 03-2014

DOI: 10.1016/J.FOODCHEM.2013.09.074

Abstract: The effect of dietary supplementation with Se-enriched Agaricus bisporus on cytosolic gluthathione peroxidase-1 (GPx-1), gastrointestinal specific glutathione peroxidase-2 (GPx-2), thioredoxin reductase-1 (TrxR-1) and selenoprotein P (SeP) mRNA expression and GPx-1 enzyme activity in rat colon was examined. Rats were fed for 5weeks with control diet (0.15μg Se/g feed) or Se-enriched diet fortified with selenised mushroom (1μg Se/g feed). The mRNA expression levels were found to be significantly (P<0.01) up-regulated by 1.65-fold and 2.3-fold for GPx-1 and GPx-2, respectively, but were not significantly different for TrxR-1 and SeP between the 2 diet treatments. The up-regulation of GPx-1 mRNA expression was consistent with GPX-1 activity level, which was significantly (P<0.05) increased by 1.77-fold in rats fed with the Se-enriched diet compared to the control diet. The results showed that selenised A. bisporus can positively increase GPx-1 and GPx-2 gene expression and GPx-1 enzyme activity in rat colon.

The Effect of Heat Stress on Respiratory Alkalosis and Insulin Sensitivity in Cinnamon Supplemented Pigs

Publisher: MDPI AG

Date: 15-04-2020

DOI: 10.3390/ANI10040690

Abstract: With increases in the frequency, intensity and duration of heat waves forecast plus expansion of tropical agriculture, heat stress (HS) is both a current and an emerging problem. As cinnamon has been shown to increase insulin sensitivity, which is part of the adaptive response to HS, the aim of this experiment was to determine if cinnamon could improve insulin sensitivity and ameliorate HS in grower pigs. In a 2 × 2 factorial design, 36 female Large White × Landrace pigs were fed control (0%) vs. cinnamon (1.5%) diets and housed for 7 day under thermoneutral (20 °C, TN) vs. HS conditions (8 h 35 °C/16 h 28 °C, 35% relative humidity). At the completion of the challenge, insulin sensitivity was assessed by an intravenous glucose tolerance test (IVGTT). Heat stress increased parameters such as respiration rate and rectal temperature. Furthermore, biochemical changes in blood and urine indicated the pigs were experiencing respiratory alkalosis. Minimal modelling of parameters of insulin sensitivity showed that HS pigs had a lower insulin response to the IVGTT and improved insulin sensitivity. Cinnamon had additive effects with heat stress, reflected in lowering the insulin area under curve (AUC) and elevated insulin sensitivity compared to TN. However, this apparent improvement in insulin sensitivity did not ameliorate any of the other physiological symptoms of HS.

Repeatability of pig body composition measurements using dual energy X-ray absorptiometry and influence of animal size and subregional analyses

Publisher: CSIRO Publishing

Date: 2006

DOI: 10.1071/EA04279

Abstract: Fifteen Large White × Landrace male pigs were used to investigate the influence of animal size and subregional analysis technique on dual energy X-ray absorptiometry body composition measurements and their repeatability. Pigs were scanned in triplicate with an Hologic QDR4500A X-ray absorptiometer at the beginning of the study (3 weeks of age, liveweight 5–10 kg) and then every 4 weeks until 19 weeks of age. Measurements made by dual energy X-ray absorptiometry included total tissue mass, lean tissue mass, fat tissue mass and bone mineral content. The QDR4500 software allows the scanned image to be ided into head, arms, legs and trunk using an in-built regional analysis grid that contains algorithms unique to each region. Different regional grid manipulations were performed at each scan to evaluate the effects of incorporating subregions into a whole body analysis over time. The dual energy X-ray absorptiometry measurements were highly repeatable and measurement repeatability improved as animal size increased. When results were averaged across regional grid placement and scan time, the most repeatable measurement was total tissue mass (CV = 0.21%), followed by lean tissue mass (CV = 0.59%), bone mineral content (CV = 2.50%) and fat tissue mass (CV = 2.71%). The placement of the regional analysis grid influenced the repeatability of all measurements except for total weight however, this influence reduced with increasing animal size. It is recommended that the body of the scan image be positioned in the arm region and the head in the head region of the software regional analysis grid to measure whole body composition in pigs because it provides the most repeatable measure overall. Animal placement into the trunk region or utilising the full regional analysis option as specified by the manufacturer provided less repeatable results.

Consumption of raw brown onions variably modulate plasma lipid profile and lipoprotein oxidation in pigs fed a high-fat diet

Publisher: Wiley

Date: 11-10-2005

DOI: 10.1002/JSFA.1976

The effect of handling pre-slaughter and carcass processing rate post-slaughter on pork quality.

Publisher: Elsevier BV

Date: 12-1998

DOI: 10.1016/S0309-1740(98)00055-2

Abstract: Forty-eight male crossbred (Large White-Landrace) pigs were used in a 2×2 factorial design to determine the effect of pre-slaughter handling (minimal and negative handling prior to slaughter) and the rate of carcass processing post-slaughter [normal rate (45min) and delayed rate (70min) from time of exsanguination to carcass entering the chiller] on muscle glycolysis and pork quality. Pigs negatively (using an electric goad) handled at the abattoir just prior to slaughter had lower muscle glycogen concentrations in the Longissimus thoracis (LT) and the Biceps femoris (BF) at all times post-slaughter and lower lactic acid at 5, 45 and 70min post-slaughter compared to pigs minimally (no use of electric goads) handled prior to slaughter. Negative handling of pigs just prior to slaughter also resulted in pork which had higher surface exudate and a higher incidence of PSE compared with pigs minimally handled prior to slaughter. A prolonged rate of carcass processing resulted in reduced muscle glycogen in the LT and BF at all times post-slaughter. Delays in carcass processing rate also affected pork quality, as the meat was paler in comparison with pig carcasses that were processed without any delays. The results from this experiment have indicated that the use of electric goads to move pigs at the abattoir, and delays in carcass processing post-slaughter, can have a detrimental influence on ultimate pork quality.

Bioavailability of selenium from selenium‐enriched milk assessed in the artificially reared neonatal pig

Publisher: Wiley

Date: 06-2008

DOI: 10.1111/J.1747-0080.2008.00259.X

Differential effects of natural palm oil, chemically- and enzymatically-modified palm oil on weight gain, blood lipid metabolites and fat deposition in a pediatric pig model

Publisher: Springer Science and Business Media LLC

Date: 18-05-2011

DOI: 10.1186/1475-2891-10-53

Emerging technologies with the potential to improve feed efficiency in swine

Publisher: Wageningen Academic Publishers

Date: 2012

DOI: 10.3920/978-90-8686-756-1_13

IGF feedback effects on growth hormone secretion in ewes: evidence for action at the pituitary but not the hypothalamic level.

Publisher: Bioscientifica

Date: 02-1995

DOI: 10.1677/JOE.0.1440323

Abstract: The putative negative feedback effects of IGF-I and IGF-II on GH secretion were tested by intracerebroventricular (icv) and intrapituitary administration to sheep. Over two consecutive days, serial jugular blood s les were taken at 10 min intervals for 6 h from ewes ( n =3/group) fitted with indwelling stainless steel cannulae into the lateral or third cerebral ventricles. The sheep were injected (icv) with either vehicle or purified ovine IGF-I (2, 4 or 8 μg). IGF-I injection had no effect on plasma GH secretion. Serial blood s les were taken from a second group of nine ewes in which ovine or recombinant human (rh) IGF-I was infused (2·5 μg/h for 2 h) into the third ventricle once again, IGF-I failed to affect the episodic pattern of GH secretion. Three ewes fitted with indwelling stainless steel cannulae placed in the anterior pituitary gland were consecutively infused with either ovine or rhIGF-I (2·5 μg/h for 2 h) or vehicle. Plasma GH concentrations were suppressed in 3/3 sheep from 1–1·5 h after the commencement of infusion and GH levels remained low for the remainder of the s ling period. In another group of five ewes synergistic effects of IGF-I and IGF-II on GH secretion were tested by icv infusion of rhIGF-I, rhIGF-II, or rhIGF-I+rhIGF-II (5 μg/h for 2 h) or vehicle (sterile 10 mm HCl/saline). Each sheep received each treatment in a randomised design. Infusion (icv) of IGF-I and IGF-II alone or in combination failed to alter GH secretion. These observations suggest that IGF-I derived from peripheral tissues may modulate GH release at the pituitary level but that IGF-I acts neither alone nor in conjunction with IGF-II as a negative feedback regulator of GH secretion via the hypothalamus in the ewe. Journal of Endocrinology (1995) 144, 323–331

Influence of photoperiod and gonadal status on food intake, adiposity, and gene expression of hypothalamic appetite regulators in a seasonal mammal

Publisher: American Physiological Society

Date: 2007

DOI: 10.1152/AJPREGU.00417.2006

Abstract: We studied the effects of photoperiod on metabolic profiles, adiposity, and gene expression of hypothalamic appetite-regulating peptides in gonad-intact and castrated Soay rams. Groups of five to six animals were studied 6, 18, or 30 wk after switching from long photoperiod (LP: 16 h of light) to short photoperiod (SP: 8 h of light). Reproductive and metabolic indexes were measured in blood plasma. Expression of neuropeptide Y (NPY), proopiomelanocortin (POMC), and leptin receptor (ObRb) in the arcuate nucleus was measured using in situ hybridization. Testosterone levels of intact animals were low under LP, increased to a peak at 16 wk under SP, and then declined. Voluntary food intake (VFI) was high under LP in both intact and castrated animals, decreased to a nadir at 12–16 wk under SP, and then recovered, but only in intact rams as the reproductive axis became photorefractory to SP. NPY gene expression varied positively and POMC expression varied negatively with the cycle in VFI, with differences between intact and castrate rams in the refractory phase. ObRb expression decreased under SP, unrelated to changes in VFI. Visceral fat weight also varied between the intact and castrated animals across the cycle. We conclude that 1) photoperiodic changes in VFI reflect changes in NPY and POMC gene expression, 2) changes in ObRb gene expression are not necessarily determinants of changes in VFI, 3) gonadal status affects the pattern of VFI that changes with photoperiod, and 4) in the absence of gonadal factors, animals can eat less but gain adiposity.

Feeding Sows Lucerne, or Diets with Similar Energy and Nutritional Profiles to Lucerne, Improves the Pre-Weaning Performance of Piglets

Publisher: MDPI AG

Date: 15-11-2021

DOI: 10.3390/AGRICULTURE11111146

Abstract: Feeding fibre and branched-chain amino acids (BCAA) to sows during late gestation and lactation, respectively, have been shown to improve litter weights at weaning. Therefore, supplementing primiparous sow diets with lucerne chaff, a feedstuff high in fibre, BCAA and tryptophan, may improve the performance of their progeny. Experiment 1 investigated the effects of feeding primiparous sows (n = 118) either (i) a control diet (CON) (ii) CON with 1 kg of lucerne chaff (LUC) or (iii) a diet formulated to have a similar digestible energy and BCAA content as CON + LUC (SIMLUC 1) until farrowing. The LUC and SIMLUC 1 diets improved day 21 litter weight (p = 0.055). In Experiment 2 primiparous (n = 111) and multiparous sows (parities 2–5, n = 112) were fed either the CON or SIMLUC diet (SIMLUC 2) from day 110 of gestation to farrowing (PreF), or to day 10 post-farrowing (PreF + PostF). The SIMLUC 2 diet tended to be more beneficial to primiparous compared to multiparous sows when fed PreF, as indicated by an interaction between diet and parity on day 21 average piglet weights (p = 0.078). Overall, SIMLUC is most effective in improving primiparous progeny performance as evidenced by their D21 weights.

Metabolic and production responses to different porcine somatotropin injection regimes in pigs

Publisher: CSIRO Publishing

Date: 2002

DOI: 10.1071/AR01141

Abstract: Thirty-two female crossbred pigs (initial weight 75 kg) were used in this experiment to investigate the effect of differing porcine somatotropin (pST) regimes on growth performance. Pigs were kept in in idual pens and fed ad libitum a wheat-based diet formulated to contain 14.4 MJ DE/kg and 180 g ideal protein/kg. Treatments were daily injection with saline (Sal), daily injection with pST (5 mg) (D), bi-daily injection with pST (10 mg) (2D), and injection with pST (12 mg) every Monday, Wednesday, and Friday (MWF). On the days that pigs were not receiving pST they were injected with saline (0.5 mL). All pST regimes caused a reduction (P 0.001) in feed intake and feed conversion ratio (FCR), although there was no significant effect on average daily gain. Feed intake decreased after the first injection and, for the D group, remained low and constant. Feed intake for the 2D group was also reduced but not to as great an extent as for the D group. Feed intake in the MWF group showed a clear temporal response, being low during the week but increasing over the latter part of the weekend. Thus, feed intake from Sunday until Monday mornings was 400 g greater (P 0.001) than from Saturday until Sunday mornings. Therefore, it appears that the effects of pST on feed intake may be reduced when a 3-day interval is used between injections. Back fat was significantly reduced with all pST injection regimes, whereas estimated fat deposition was only significantly reduced in the D and 2D pigs. Plasma urea nitrogen was increased, whereas glucose was decreased during pST administration and the average concentrations of both metabolites during the first week of administration were strongly related (P 0.001) to FCR over the full 3 weeks of the study (R = 0.733 and -0.683, respectively). Although daily injection with pST appears to give the greatest reduction in feed intake and fat deposition, an alternative pST injection regime involving less frequent pST injections can also improve growth performance similarly in finisher pigs.

The Impact of Heat Stress on Immune Status of Dairy Cattle and Strategies to Ameliorate the Negative Effects

Publisher: MDPI AG

Date: 27-12-2022

DOI: 10.3390/ANI13010107

Abstract: Heat stress (HS) is well known to influence animal health and livestock productivity negatively. Heat stress is a multi-billion-dollar global problem. It impairs animal performance during summer when animals are exposed to high ambient temperatures, direct and indirect solar radiations, and humidity. While significant developments have been achieved over the last few decades to mitigate the negative impact of HS, such as physical modification of the environment to protect the animals from direct heat, HS remains a significant challenge for the dairy industry compromising dairy cattle health and welfare. In such a scenario, it is essential to have a thorough understanding of how the immune system of dairy cattle responds to HS and identify the variable responses among the animals. This understanding could help to identify heat-resilient dairy animals for breeding and may lead to the development of climate resilient breeds in the future to support sustainable dairy cattle production. There are sufficient data demonstrating the impact of increased temperature and humidity on endocrine responses to HS in dairy cattle, especially changes in concentration of hormones like prolactin and cortisol, which also provide an indication of the likely im-pact on the immune system. In this paper, we review the recent research on the impact of HS on immunity of calves during early life to adult lactating and dry cows. Additionally, different strategies for amelioration of negative effects of HS have been presented.

A Comparative Investigation on Phenolic Composition, Characterization and Antioxidant Potentials of Five Different Australian Grown Pear Varieties

Publisher: MDPI AG

Date: 20-01-2021

DOI: 10.3390/ANTIOX10020151

Abstract: Pear (Pyrus communis L.) is widely spread throughout the temperate regions of the world, such as China, America and Australia. This fruit is popular among consumers due to its excellent taste and perceived health benefits. Various bioactive compounds, which contribute to these health benefits, have been detected in the pear fruits, including a range of phenolic compounds. Five Australian grown pear varieties, which include Packham’s Triumph, Josephine de Malines, Beurre Bosc, Winter Nelis and Rico were selected for this study to examine the phenolic compounds in pears. Beurre Bosc exhibited the highest total polyphenol content (TPC) (3.14 ± 0.02 mg GAE/g), total tannin content (TTC) (1.43 ± 0.04 mg CE/g) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) (5.72 ± 0.11 mg AAE/g), while the Josephine de Malines variety was high in total flavonoid content (TFC) (1.53 ± 0.09 mg QE/g), ferric reducing antioxidant power (FRAP) (4.37 ± 0.04 mg AAE/g), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (4.44 ± 0.01 mg AAE/g) and total antioxidant capacity (TAC) (5.29 ± 0.09 mg AAE/g). The liquid chromatography coupled with electrospray-ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS) data indicate that a total of 73 phenolic compounds were detected in Beurre Bosc (37 compounds), Josephine de Malines (34), Rico (22), Packham’s Triumph (15) and Winter Nelis (9), respectively. From HPLC-PDA quantification, the Beurre Bosc pear variety showed significantly higher in phenolic acids (chlorogenic acid 17.58 ± 0.88 mg/g) and while flavonoids were significantly higher in Josephine de Malines (catechin 17.45 ± 1.39 mg/g), as compared to other pear varieties. The analyses suggest that the Australian grown pears might contain an ideal source of phenolic compounds which benefit human health. The information provided by the present work can serve as practical supporting data for the use of pears in the nutraceutical, pharmaceutical and food industries.

Gut Microbiota-Polyphenol Interactions in Chicken: A Review

Publisher: MDPI AG

Date: 11-08-2020

DOI: 10.3390/ANI10081391

Abstract: The gastrointestinal tract of the chicken harbors very complex and erse microbial communities including both beneficial and harmful bacteria. However, a dynamic balance is generally maintained in such a way that beneficial bacteria predominate over harmful ones. Environmental factors can negatively affect this balance, resulting in harmful effects on the gut, declining health, and productivity. This means modulating changes in the chicken gut microbiota is an effective strategy to improve gut health and productivity. One strategy is using modified diets to favor the growth of beneficial bacteria and a key candidate are polyphenols, which have strong antioxidant potential and established health benefits. The gut microbiota-polyphenol interactions are of vital importance in their effects on the gut microbiota modulation because it affects not only the composition of gut bacteria but also improves bioavailability of polyphenols through generation of more bioactive metabolites enhancing their health effects on morphology and composition of the gut microbiota. The object of this review is to improve the understanding of polyphenol interactions with the gut microbiota and highlights their potential role in modulation of the gut microbiota of chicken.

Colostrum Protein Isolate Increases Gut and Whole Body Growth and Plasma IGF-I in Neonatal Pigs

Publisher: Asian Australasian Association of Animal Production Societies

Date: 27-04-2011

DOI: 10.5713/AJAS.2011.90490

The Impact of Wet Fermentation on Coffee Quality Traits and Volatile Compounds Using Digital Technologies

Publisher: MDPI AG

Date: 13-01-2023

DOI: 10.3390/FERMENTATION9010068

Abstract: Fermentation is critical for developing coffee’s physicochemical properties. This study aimed to assess the differences in quality traits between fermented and unfermented coffee with four grinding sizes of coffee powder using multiple digital technologies. A total of N = 2 coffee treatments—(i) dry processing and (ii) wet fermentation—with grinding levels (250, 350, 550, and 750 µm) were analysed using near-infrared spectrometry (NIR), electronic nose (e-nose), and headspace/gas chromatography–mass spectrometry (HS-SPME-GC-MS) coupled with machine learning (ML) modelling. Most overtones detected by NIR were within the ranges of 1700–2000 nm and 2200–2396 nm, while the enhanced peak responses of fermented coffee were lower. The overall voltage of nine e-nose sensors obtained from fermented coffee (250 µm) was significantly higher. There were two ML classification models to classify processing and brewing methods using NIR (Model 1) and e-nose (Model 2) values as inputs that were highly accurate (93.9% and 91.2%, respectively). Highly precise ML regression Model 3 and Model 4 based on the same inputs for NIR (R = 0.96) and e-nose (R = 0.99) were developed, respectively, to assess 14 volatile aromatic compounds obtained by GC-MS. Fermented coffee showed higher 2-methylpyrazine (2.20 ng/mL) and furfuryl acetate (2.36 ng/mL) content, which induces a stronger fruity aroma. This proposed rapid, reliable, and low-cost method was shown to be effective in distinguishing coffee postharvest processing methods and evaluating their volatile compounds, which has the potential to be applied for coffee differentiation and quality assurance and control.

Dietary conjugated linoleic acid improves carcass leanness without altering meat quality in the growing pig

Publisher: CSIRO Publishing

Date: 2005

DOI: 10.1071/EA04144

Abstract: One constraint facing the pig industry is that ad libitum feeding can often result in high levels of body fat and technologies which can reduce the ratio of lean to fat deposition in the pig are continually being explored. Conjugated linoleic acids have been shown to decrease body fat content in pigs. Therefore, the aim of this study was to determine whether dietary conjugated linoleic acids supplementation has any effect on meat quality and carcass characteristics in finisher pigs. Sixty female crossbred (Large White × Landrace) pigs (average initial weight 56.6 ± 1.9 kg and average initial P2 backfat 11.4 ± 1.3 mm) were used in the present study. Pigs were in idually housed and randomly allocated to 1 of 6 dietary treatments: 0, 0.125, 0.25, 0.50, 0.75 or 1.0% (w/w) of conjugated linoleic acids-55. The wheat-based diets were formulated to contain 14.3 MJ DE and 9.3 g available lysine per kg and were fed ad libitum for 8 weeks. Pigs were slaughtered and meat quality was determined on the longissimus thoracis using standard techniques. Dietary conjugated linoleic acids reduced subcutaneous back fat in a linear manner with effects being most pronounced in the middle back fat layer. There was also a linear (P .001) decrease in intramuscular fat with increasing dietary conjugated linoleic acids supplementation. However, there was no effect of conjugated linoleic acids on subjective measures of marbling of the loin. Also, loin muscle ultimate pH (P = 0.94), lightness values (P = 0.46) subjective colour scores (P = 0.79), cooking loss (P = 0.71), drip loss (P = 0.40), shear force (P = 0.61) and subjective measures of wetness/firmness (P = 0.19) were unaffected. Dietary conjugated linoleic acids did not alter oxidation, as measured by the level of TBARs at day 1 post-slaughter (P = 0.38) or after 9 days of simulated retail display (P = 0.35). These data confirm that dietary conjugated linoleic acids can improve carcass quality by decreasing back fat depths without having any detrimental effects on meat quality.

Dual energy X-ray absorptiometry predicts the effects of dietary protein on body composition of pigs

Publisher: CSIRO Publishing

Date: 2006

DOI: 10.1071/EA04266

Abstract: The present study investigated the potential of dual energy X-ray absorptiometry to determine the effect of dietary protein on live pig body composition compared with the standard methods. The experiment utilised 48 Large White × Landrace gilts, stratified on liveweight at 17 weeks of age (initial liveweight about 60 kg), and allocated within strata to diets containing either 85 or 100% of dietary protein requirements. The diets were fed ad libitum and contained 14.6 or 16.7% protein, respectively. Pigs were housed in idually to allow weekly measurement of feed intake and liveweight. A Hologic QDR4500A dual energy X-ray absorptiometer was used to determine lean, fat and ash composition of pigs initially and 4 weeks later at the end of the experiment. Daily gain was increased by about 200 g in pigs fed the protein-adequate diet, but feed intake was not affected. Therefore, pigs fed adequate protein had a lower feed conversion ratio (2.92 v. 3.52 g/g, P .001). Feeding the protein-adequate diet increased lean deposition by about 150 g/day (577 v. 429 g/day, P .001) and ash deposition by about 4 g/day (28.7 v. 25.0 g/day, P .001), but did not affect fat accretion. This resulted in carcasses with a higher lean content (54.0 v. 50.7 kg, P .001) and ash content (2.35 v. 2.28 kg, P .05) but unaltered fat content. Therefore, an inadequate level of dietary protein leads to suboptimal growth in lean tissue and bone mineral, with no pronounced effect on fat. These observations were substantiated by chemical analysis and available corrective equations were useful in correcting differences between dual energy X-ray absorptiometry outputs and chemical values. After correction, dual energy X-ray absorptiometry estimates differed less than 5% from the chemical values for lean, protein, water and ash and 10% for lipid. In addition, reduced standard error of the differences around most dual energy X-ray absorptiometry measurements, relative to chemical analysis, allowed small changes in body composition to be detected with increased confidence. These data support the efficacy of using dual energy X-ray absorptiometry in nutritional studies of pigs.

Dietary Lecithin Supplementation Can Improve the Quality of the M. Longissimus thoracis.

Publisher: MDPI AG

Date: 25-11-2015

DOI: 10.3390/ANI5040405

Optimizing conditions for DNA isolation fromPinus radiata

Publisher: Springer Science and Business Media LLC

Date: 04-1998

DOI: 10.1007/BF02822773

Output of selenium in milk, urine, and feces is proportional to selenium intake in dairy cows fed a total mixed ration supplemented with selenium yeast

Publisher: American Dairy Science Association

Date: 10-2010

DOI: 10.3168/JDS.2010-3186

Abstract: Fifteen rumen fistulated Holstein cows in late lactation and fed a total mixed ration offered ad libitum were supplemented with Se yeast to provide 0, 11, 20, 30, or 42 mg of supplemental Se/day to test the hypothesis that amounts of Se secreted in milk, excreted in urine and feces, and apparently retained in tissues would increase in direct proportion to Se intake. One-half of the yeast supplement was placed directly into the rumen through the fistula of each cow just before milking in the morning and again in the evening, and estimates of average daily excretion of Se were made using total collections of urine and feces from 25 to 31 d after treatments commenced. Amounts of Se secreted daily in milk and apparently retained in tissues increased linearly with average daily intake of Se. The amount of Se excreted in feces and total excretion of Se in urine plus feces increased curvilinearly with Se intake, such that proportionately less Se was excreted as the amount of Se fed increased. On average, total Se excretion accounted for 66%, Se secretion in milk accounted for 17%, and Se apparently retained in tissues accounted for 17% of total Se intake by cows. Thus, in herds fed large amounts of Se yeast, most of the Se will be excreted and retained on-farm. High concentrations of Se will be found where urine and feces accumulate (e.g., yards and effluent ponds), and effluent management practices must be tailored to avoid environmental issues.

LC-ESI-QTOF/MS Profiling of Australian Mango Peel By-Product Polyphenols and Their Potential Antioxidant Activities

Publisher: MDPI AG

Date: 18-10-2019

DOI: 10.3390/PR7100764

Abstract: Mango (Mangifera indica L.) is one of the most important fruits in the world. Mango peel is an important by-product that is rich in polyphenols and it could have high economic value if it is effectively utilized. Phenolic characterization is an essential step in the commercial utilization of mango peel by-products as food ingredients. Herein, qualitative and quantitative analyses of two Australian mango peel “Keitt” and “Kensington Pride” (K& P) by-products were conducted while using liquid chromatography coupled to electrospray ionisation and quadrupole time of flight mass spectrometry (LC-ESI-QTOF/MS) and high-performance liquid chromatography coupled to photodiode array detector (HPLC-PDA). A total of 98 polyphenols compounds were tentatively identified in both Keitt peel and K& P peel extracts, with greater concentrations of these compounds being detected in Keitt peel. The total phenolic content (TPC), total flavonoid content (TFC), and a total tannin content (TTC) were determined. The antioxidant activity of mango peel by-products was determined while using 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay, ferric reducing antioxidant power (FRAP) assay, and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging assay. Keitt peel contained higher concentrations of total phenolic compounds, flavonoids, and tannins and had higher antioxidant capacity in DPPH, FRAP, and ABTS assays as compared to K& P peel. In HPLC-PDA quantification, the predominant phenolic compounds in Keitt peel and K& P peel were catechin (62.32 ± 0.01 mg/gd.w.) and syringic acid (17.78 ± 0.01 mg/gd.w).

Dietary Monounsaturated Fat in Early Life Regulates IGFBP2: Implications for Fat Mass Accretion and Insulin Sensitivity

Publisher: Wiley

Date: 12-2011

DOI: 10.1038/OBY.2011.55

Abstract: The aim of this study was to investigate effects of dietary supplementation with fat or sugar on body composition (BC) and insulin sensitivity (IS) in maturing pigs. Fifty newborn pigs randomized to a control diet or 18% saturated fat (SF), 18% monounsaturated fat (MUF), 18% mixed fat (MF), or 50% sucrose (SUC), from 1 to 16 weeks of age. Outcomes included weight gain, BC (dual energy X-ray absorptiometry, DXA), IS (fasting insulin and hyperinsulinaemic-euglycaemic cl s), fasting Non-Esterified Fatty Acid (NEFA) concentrations, and mRNA expression of genes involved in lipogenesis and IS in skeletal muscle (SM), subcutaneous (SAT), and visceral adipose tissue (VAT). In vitro studies examined direct effects of fatty acids on insulin-like growth factor-binding protein 2 (IGFBP2) mRNA in C2C12 myotubes. While SUC-fed pigs gained most weight (due to larger quantities consumed P < 0.01), those fed fat-enriched diets exhibited more weight gain per unit energy intake (P < 0.001). Total (P = 0.03) and visceral (P = 0.04) adiposity were greatest in MUF-fed pigs. Whole-body IS was decreased in those fed fat (P = 0.04), with fasting insulin increased in MUF-fed pigs (P = 0.03). SM IGFBP2 mRNA was increased in MUF-fed pigs (P = 0.009) and, in all animals, SM IGFBP2 mRNA correlated with total (P = 0.007) and visceral (P = 0.001) fat, fasting insulin (r = 0.321 P = 0.03) and change in NEFA concentrations (r = 0.285 P = 0.047). Furthermore, exposure of in vitro cultured myotubes to MUF, but not SF, reduced IGFBP2 mRNA suggesting a converse direct effect. In conclusion, diets high in fat, but not sugar, promote visceral adiposity and insulin resistance in maturing pigs, with evidence that fatty acids have direct and indirect effects on IGFBP2 mRNA expression in muscle.

Short-Term Infusion of LongR3 Insulin-like Growth Factor (IGF)-I Decreases Hepatic IGF-I mRNA but Not IGF Binding Protein-3 mRNA Expression in Pigs

Publisher: Elsevier BV

Date: 04-2002

DOI: 10.1006/GCEN.2002.7796

The Importance of Dietary Antioxidants on Oxidative Stress, Meat and Milk Production, and Their Preservative Aspects in Farm Animals: Antioxidant Action, Animal Health, and Product Quality—Invited Rev

Publisher: MDPI AG

Date: 24-11-2022

DOI: 10.3390/ANI12233279

Abstract: The biological effects of oxidative stress and associated free radicals on farm animal performance, productivity, and product quality may be managed via dietary interventions—specifically, the provision of feeds, supplements, and forages rich in antioxidants. To optimize this approach, it is important first to understand the development of free radicals and their contributions to oxidative stress in tissue systems of farm animals or the human body. The interactions between prooxidants and antioxidants will impact redox homeostasis and, therefore, the well-being of farm animals. The impact of free radical formation on the oxidation of lipids, proteins, DNA, and biologically important macromolecules will likewise impact animal performance, meat and milk quality, nutritional value, and longevity. Dietary antioxidants, endogenous antioxidants, and metal-binding proteins contribute to the ‘antioxidant defenses’ that control free radical formation within the biological systems. Different bioactive compounds of varying antioxidant potential and bio-accessibility may be sourced from tailored feeding systems. Informed and successful provision of dietary antioxidants can help alleviate oxidative stress. However, knowledge pertaining to farm animals, their unique biological systems, and the applications of novel feeds, specialized forages, bioactive compounds, etc., must be established. This review summarized current research to direct future studies towards more effective controls for free radical formation/oxidative stress in farm animals so that productivity and quality of meat and milk can be optimized.

Abattoir Factors Influencing the Incidence of Dark Cutting in Australian Grain-Fed Beef

Publisher: MDPI AG

Date: 10-02-2021

DOI: 10.3390/ANI11020474

Abstract: The aim of this study was to evaluate the effect of carcass traits, lairage time and weather conditions during lairage and abattoir factors that impact the incidence of dark cutting in 142,228 grain-fed carcasses, as defined by Meat Standards Australia (MSA) guidelines. This study was conducted over a 12-month period analysing data from cattle that were supplied from seven feedlots and processed at three abattoirs. Abattoir data indicated that the average incidence of dark cutting within the study was 2.8%. Increased wind speeds (WSs) and rain during lairage at the abattoir was associated with an increased risk of dark cutting, whereas variation in ambient temperature and/or relative humidity did not influence dark cutting. Heavier carcasses with whiter fat, larger hump heights, more rib fat, higher marble scores and lower ossification had lower incidences of dark cutting. The factors abattoir, time in lairage, time to grading and grader within Abattoir had significant effects on the incidence of dark cutting. The results from this study suggest that reducing the time in lairage and increasing the time between slaughter and grading are the two major ways to reduce dark cutting in MSA carcasses.

Altered set-point of the hypothalamus determines effects of cortisol on food intake, adiposity, and metabolic substrates in sheep

Publisher: Elsevier BV

Date: 2010

DOI: 10.1016/J.DOMANIEND.2009.07.006

Abstract: Chronic elevation of glucocorticoid concentrations is detrimental to health. We investigated effects of chronic increase in plasma cortisol concentrations on energy balance and endocrine function in sheep. Because food intake and reproduction are regulated by photoperiod, we performed experiments in January (JAN) and August (AUG), when appetite drive is either high or low, respectively. Ovariectomized ewes were treated (intramuscularly) daily with 0.5mg Synacthen Depot(R) (synthetic adrenocorticotropin: ACTH) or saline for 4 wk. Blood s les were taken to measure plasma concentrations of cortisol, luteinising hormone (LH), follicle-stimulating hormone (FSH), growth hormone (GH), leptin, insulin, and glucose. Adrenocorticotropin treatment increased concentrations of cortisol. During JAN, treatment reduced food intake transiently, but increased food intake in AUG. Leptin concentrations were reduced and glucose concentrations were greater in AUG, and insulin concentrations were similar throughout the year. Treatment with ACTH increased leptin concentrations in AUG only, whereas insulin concentrations increased in JAN only. Synacthen treatment increased glucose concentrations, with a greater effect in JAN. Changes in truncal adiposity and ACTH-induced cortisol secretion were positively correlated in JAN and negatively correlated in AUG. Treatment reduced the plasma LH pulse frequency in JAN and AUG, with an effect on pulse litude in JAN only. Treatment did not affect plasma GH or FSH concentrations. We conclude that chronically elevated cortisol concentrations can affect food intake, adiposity, and reproductive function. In sheep, effects of chronically elevated cortisol concentrations on energy balance and metabolism depend upon metabolic setpoint, determined by circannual rhythms.

Screening and characterization of phenolic compounds from australian grown bananas and their antioxidant capacity

Publisher: MDPI AG

Date: 25-09-2021

DOI: 10.3390/ANTIOX10101521

Abstract: Bananas are an essential source of staple food and fruit worldwide and are widely regarded as the world’s largest fruit crop, with more than 100 million tons total annual production. Banana peel, a by-product that represents about 40% of the entire banana’s weight, and pulp are rich in bioactive compounds and have a high antioxidant capacity. As the production of polyphenols in fruit and vegetables is highly dependent on environmental conditions, genetic factors, and the level of maturity, this study aims to characterize six Australian banana cultivars in various stages of ripening for their phenolic compounds using the liquid chromatography-electrospray ionization quadrupole time of flight mass spectrometry (LC-ESI-QTOF-MS/MS), polyphenols quantification with the high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA), and their antioxidant capacity. All bananas were analysed for total polyphenols content (TPC), total flavonoids content (TFC), and total tannin content (TTC) and their antioxidant activities. Ripe Ducasse peel and pulp contained the highest amounts of total polyphenols content (1.32 and 1.28 mg gallic acid equivalent (GAE) per gram of s le), total tannin contents (3.34 mg catechin equivalent (CE) per gram of s le), and free radical scavenging capacity (106.67 mg ascorbic acid equivalent (AAE) per g of s le). In contrast, ripe Plantain peel had the greatest total flavonoids (0.03 mg quercetin equivalent (QE) per g of s le). On the other hand, unripe Ladyfinger pulp possessed the highest total antioxidant activity (1.03 mg AAE/g of s le). There was a positive correlation between flavonoids and antioxidant activities. By using LC-ESI-QTOF-MS/MS, a total of 24 phenolic compounds were tentatively characterized in this research, including six phenolic acids, 13 flavonoids, and five other polyphenols. Quantification of phenolic compounds by the high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA) revealed a higher content of phenolic acids. These findings confirmed that banana peel and pulp have considerable antioxidant activity and can be employed in human food and animal feed for variant health enhancement uses.

Climate Change and Goat Production: Enteric Methane Emission and Its Mitigation

Publisher: MDPI AG

Date: 07-12-2018

DOI: 10.3390/ANI8120235

Abstract: The ability of an animal to cope and adapt itself to the changing climate virtually depends on the function of rumen and rumen inhabitants such as bacteria, protozoa, fungi, virus and archaea. Elevated ambient temperature during the summer months can have a significant influence on the basic physiology of the rumen, thereby affecting the nutritional status of the animals. Rumen volatile fatty acid (VFA) production decreases under conditions of extreme heat. Growing recent evidence suggests there are genetic variations among breeds of goats in the impact of heat stress on rumen fermentation pattern and VFA production. Most of the effects of heat stress on rumen fermentation and enteric methane (CH4) emission are attributed to differences in the rumen microbial population. Heat stress-induced rumen function impairment is mainly associated with an increase in Streptococcus genus bacteria and with a decrease in the bacteria of Fibrobactor genus. Apart from its major role in global warming and greenhouse effect, enteric CH4 is also considered as a dietary energy loss in goats. These effects warrant mitigating against CH4 production to ensure optimum economic return from goat farming as well as to reduce the impact on global warming as CH4 is one of the more potent greenhouse gases (GHG). The various strategies that can be implemented to mitigate enteric CH4 emission include nutritional interventions, different management strategies and applying advanced biotechnological tools to find solution to reduce CH4 production. Through these advanced technologies, it is possible to identify genetically superior animals with less CH4 production per unit feed intake. These efforts can help the farming community to sustain goat production in the changing climate scenario.

Intracerebroventricular infusion of leptin elevates the secretion of luteinising hormone without affecting food intake in long-term food-restricted sheep, but increases growth hormone irrespective of bodyweight

Publisher: Bioscientifica

Date: 2001

DOI: 10.1677/JOE.0.1680067

Abstract: Leptin can act as a satiety factor and exert neuroendocrine effects, but most studies have been performed in fasted animals. We aimed to determine the effect of chronic under-nutrition on the response to a 3-day intracerebroventricular infusion of leptin with regard to food intake and the secretion of pituitary hormones. Ovariectomised ewes (n=6) had a mean (+/-s.e.m. ) bodyweight of 56+/-0.8 kg on a diet available ad libitum (ad lib) or 33.4+/-1 kg on a restricted diet. The differential bodyweight was achieved by dietary means over a period of 6 months prior to the commencement of the study. Leptin (4 microg/h) or vehicle (artificial cerebrospinal fluid (aCSF)) was infused into the third cerebral ventricle for 3 days. Blood s les were taken prior to commencement and on day 3 of infusion for the assay of plasma hormone levels. The experiment was repeated one week later in a cross-over design. Food intake and metabolic status were monitored daily. The luteinising hormone (LH) pulse litude was lower (P .05) but plasma growth hormone (GH) levels were higher (P .05) in the food-restricted animals. Plasma levels of glucose, lactate, insulin, urea and triglycerides were similar in the two groups but non-esterified fatty acid levels were higher (P .01) in the animals on an ad lib diet. Leptin reduced (P .05) food intake only in the animals fed an ad lib diet. Leptin increased (P .05) the secretion of LH in the food-restricted group only and increased (P .05) GH irrespective of bodyweight. In conclusion, leptin does not alter food intake in animals on a restricted diet but can increase the secretion of LH in the same animals. The treatment of leptin was not sufficient to reduce plasma GH levels in the food-restricted animals, suggesting that other factors or mechanisms must be involved in the regulation of this axis.

Effect of dietary protein on body composition and insulin resistance using a pig model of the child and adolescent

Publisher: Wiley

Date: 06-2008

DOI: 10.1111/J.1747-0080.2008.00263.X

Dietary antioxidants at supranutritional doses improve oxidative status and reduce the negative effects of heat stress in sheep1,2

Publisher: Oxford University Press (OUP)

Date: 08-2014

DOI: 10.2527/JAS.2014-7714

Abstract: The present study was undertaken to investigate the impact of heat (thermal) stress and dietary antioxidant supplementation on the oxidative and physiological status of sheep. Twenty-four Merino × Poll Dorset crossbred ewes were housed in 1 of 2 climatic chambers (thermoneutral or heat stress) and offered either a control (10 IU vitamin E/kg DM and 0.24 mg Se/kg DM) or high antioxidant (100 IU vitamin E/kg DM and 1.20 mg Se/kg DM) diet. The sheep were exposed to 2 thermal (temperature) treatments (thermoneutral [TN]: 18-21°C and 26-30% relative humidity and heat stress [HS]: 28-40°C and 40-50% relative humidity) for 2 wk in a single reversal design. After 1 wk of dietary treatment, animals in 1 chamber were subjected to HS for 1 wk, with the temperature being increased to 40°C between 0900 and 1700 h and then maintained at 28°C overnight. Those sheep in the TN group were maintained at 18 to 21°C. Physiological parameters were recorded 4 times a day (0900, 1300, 1700, and 2100 h) and blood s les were collected on d 1 and 7 of heat treatment. Plasma s les and red blood cell lysates were assayed for oxidative stress biomarkers. The thermal treatments were then reversed and the above measures repeated. All measured physiological parameters were elevated (P < 0.001) by thermal treatment. Respiration rate was lower during HS in sheep supplemented with antioxidants as indicated by a diet × temperature × time interaction (P = 0.010). There was 13% decline (P = 0.014) in feed intake of the unsupplemented animals during HS whereas the same was maintained in sheep supplemented with high doses of antioxidants. Plasma reactive oxygen metabolites concentrations were reduced (114 vs. 85 units/dL P < 0.005) while biological antioxidant potential tended to be increased (3,688 vs. 3,985 μmol/L P = 0.070) in heat stressed sheep supplemented with antioxidants. The oxidative stress index was 30% lower (P < 0.001) in supplemented sheep (2.16 ± 0.06 arbitrary units) during HS than in unsupplemented sheep (3.12 ± 0.08 arbitrary units). Plasma advanced oxidation protein products tended (P = 0.070) to decrease in antioxidant supplemented heat stressed sheep as compared to their unsupplemented counterparts. It was concluded that heat stress negatively affects the oxidative status of sheep along with the physiological responses and some of these affects can be ameliorated through dietary antioxidants supplementation at supranutritional concentrations.

Nutritional strategies to alleviate heat stress in pigs

Publisher: CSIRO Publishing

Date: 2015

DOI: 10.1071/AN15255

Abstract: Pigs are comparatively less heat tolerant than other species of production animals, which poses challenges for stock productivity and management during seasonal heat waves that occur in summer. The issues surrounding heat and pig production are predicted to increase, based on the actions of climate change increasing the intensity, frequency and duration of heat waves. Furthermore, future growth areas of pig production are going to be in tropical regions such as South-east Asia and Latin America. Efforts by the pig to dissipate excess body heat come at a cost to health and ert energy away from growth, compromising efficient pig production. Management of heat stress requires multiple strategies, and recent research is improving the understanding of the application of nutritional strategies to ameliorate the effects of heat stress. In particular the use of feed additives is an important, flexible and economical method to alleviate heat stress and the intensive nature of pig production lends itself to the use of additives. Some specific ex les include antioxidants, betaine and chromium, which have been proved effective or being tested in mitigating some certain impacts of heat stress in pigs. The aim of this review is to summarise recent advances in the nutritional management of heat stress in pigs.

Phenolic Profiling of Five Different Australian Grown Apples

Publisher: MDPI AG

Date: 09-03-2021

DOI: 10.3390/APP11052421

Abstract: Apples (Malus domestica) are one of the most widely grown and consumed fruits in the world that contain abundant phenolic compounds that possess remarkable antioxidant potential. The current study characterised phenolic compounds from five different varieties of Australian grown apples (Royal Gala, Pink Lady, Red Delicious, Fuji and Smitten) using LC-ESI-QTOF-MS/MS and quantified through HPLC-PDA. The phenolic content and antioxidant potential were determined using various assays. Red Delicious had the highest total phenolic (121.78 ± 3.45 mg/g fw) and total flavonoid content (101.23 ± 3.75 mg/g fw) among the five apple s les. In LC-ESI-QTOF-MS/MS analysis, a total of 97 different phenolic compounds were characterised in five apple s les, including Royal Gala (37), Pink Lady (54), Red Delicious (17), Fuji (67) and Smitten (46). In the HPLC quantification, phenolic acid (chlorogenic acid, 15.69 ± 0.09 mg/g fw) and flavonoid (quercetin, 18.96 ± 0.08 mg/g fw) were most abundant in Royal Gala. The obtained results highlight the importance of Australian apple varieties as a rich source of functional compounds with potential bioactivity.

Growth hormone but not insulin-like growth factor-I improves wound strength in pigs.

Publisher: Wiley

Date: 04-1997

DOI: 10.1046/J.1524-475X.1997.50209.X

The use of biochemical measurements to identify pre-slaughter stress in pasture finished beef cattle

Publisher: MDPI AG

Date: 31-07-2019

DOI: 10.3390/ANI9080503

Abstract: This study considered the relationship between pre-slaughter stressors and plasma biomarkers in 488 pasture-raised cattle across two experiments. The design aimed to test groups consisting of steer only, heifer only, and mixed sex cattle under direct kill versus rested (14 days in abattoir holding paddocks) protocols. In Experiment One, cattle were sourced from four farms, and transported by trucks and ships on the same day. In Experiment Two, cattle were sourced from four farms where a comparison was made between marketing via two commercial saleyards or direct farm gate consignment to abattoir. Blood s les were collected at exsanguination for subsequent analyses and relation to meat quality attributes. Muscle damage, as indicated by creatine kinase, is the biomarker most correlated to ultimate pH and muscle glycogen concentrations. A two-week rest period is effective for lowering this enzyme and improving muscle glycogen concentration. Although the cattle was subjected to a range of stress inducing treatments, we found that plasma biomarkers alone appeared insufficient for use as diagnostic stress indicators.

Bioaccessibility and movement of phenolic compounds from tomato (Solanum lycopersicum) during in vitro gastrointestinal digestion and colonic fermentation.

Publisher: Royal Society of Chemistry (RSC)

Date: 2022

DOI: 10.1039/D2FO00223J

Abstract: Tomato ( Solanum lycopersicum ) is one of the most popular vegetables and has great nutritional values due to the presence of numerous bioactive compounds, especially phenolic compounds.

LC-ESI-QTOF-MS/MS Characterisation of Phenolics in Herbal Tea Infusion and Their Antioxidant Potential

Publisher: MDPI AG

Date: 10-05-2021

DOI: 10.3390/FERMENTATION7020073

Abstract: Ginger (Zingiber officinale R.), lemon (Citrus limon L.) and mint (Mentha sp.) are commonly consumed medicinal plants that have been of interest due to their health benefits and purported antioxidant capacities. This study was conducted on the premise that no previous study has been performed to elucidate the antioxidant and phenolic profile of the ginger, lemon and mint herbal tea infusion (GLMT). The aim of the study was to investigate and characterise the phenolic contents of ginger, lemon, mint and GLMT, as well as determine their antioxidant potential. Mint recorded the highest total phenolic content, TPC (14.35 ± 0.19 mg gallic acid equivalent/g) and 2,2′-azino-bis(3-e-thylbenzothiazoline-6-sulfonic acid), ABTS (24.25 ± 2.18 mg ascorbic acid equivalent/g) antioxidant activity. GLMT recorded the highest antioxidant activity in the reducing power assay, RPA (1.01 ± 0.04 mg ascorbic acid equivalent/g) and hydroxyl radical scavenging assay, •OH-RSA (0.77 ± 0.08 mg ascorbic acid equivalent/g). Correlation analysis showed that phenolic content positively correlated with the antioxidant activity. Venn diagram analysis revealed that mint contained a high proportion of exclusive phenolic compounds. Liquid chromatography coupled with electrospray ionisation and quadrupole time of flight tandem mass spectrometry (LC-ESI-QTOF-MS/MS) characterised a total of 73 phenolic compounds, out of which 11, 31 and 49 were found in ginger, lemon and mint respectively. These characterised phenolic compounds include phenolic acids (24), flavonoids (35), other phenolic compounds (9), lignans (4) and stilbene (1). High-performance liquid chromatography photometric diode array (HPLC-PDA) quantification showed that GLMT does contain a relatively high concentration of phenolic compounds. This study presented the phenolic profile and antioxidant potential of GLMT and its ingredients, which may increase the confidence in developing GLMT into functional food products or nutraceuticals.

Genotype and age effects on sheep meat production. 5. Lean meat and fat content in the carcasses of Australian sheep genotypes at 20-, 30- and 40-kg carcass weights

Publisher: CSIRO Publishing

Date: 2008

DOI: 10.1071/EA08054

Abstract: Lean meat and fat content of Australian sheep genotypes were compared at 20-, 30- and 40-kg carcass weights. Sheep comprised Poll Dorsetgrowth × Border Leicester Merino (PDg × BLM), Poll Dorsetgrowth × Merino (PDg × M), Poll Dorsetmuscling × Merino (PDm × M), Border Leicester × Merino (BL × M) and Merino × Merino (M × M) genotypes. Lambs were raised as a mixed flock under grazing and slaughtered at 4, 8, 14 and 22 months of age with each slaughter time involving ~150 mixed sex animals. At 24 h after slaughter, chilled carcasses were halved along the backbone and the right sides were used for determination of lean, fat and ash percentages using dual energy X-ray absorptiometry. Within a particular age group and genotype, animals growing at faster rates and reaching heavier carcass weights had lower carcass lean meat content than slower growing animals. Merino carcasses weighing 20 and 30 kg had similar levels of lean meat to PD × M genotypes, which was greater than that from the BL × M genotype. Second-cross PD × BLM carcasses weighing 20 kg at 4 months and 30 kg at 8 months had similar carcass fat and lean percentages to 20-kg Merino carcasses at 8 months and first-cross PD × M carcasses weighing 30 kg at 14 months, respectively. At 40-kg carcass weight, 22-month-old Merinos had similar levels of leanness to carcasses from 22-month-old PD × M animals and carcasses from 14-month-old second-cross PD × BLM animals. Carcass lean meat content decreased with increasing carcass weight and first- cross BL × M animals had the lowest carcass lean across all weight categories. There was a major acceleration in carcass fatness between 14 and 22 months associated with a reduction in muscle deposition. Results indicate that age of the animal should be taken into account when carcass lean and fat contents are compared at a particular carcass weight. Merinos will achieve weight/composition specifications at least equally well to crossbreds but will take longer with a likely increase in production costs.

Increasing Selenium Concentration in Milk: Effects of Amount of Selenium from Yeast and Cereal Grain Supplements

Publisher: American Dairy Science Association

Date: 09-2007

DOI: 10.3168/JDS.2006-800

Abstract: Two experiments were conducted to establish responses in milk Se concentrations in grazing dairy cows to different amounts of dietary Se yeast, and to determine the effects of the Se concentration of the basal diet. The hypothesis tested was that the response in milk, blood, and tissue Se concentrations to supplemental Se would not be affected by whether the Se was from the basal diet or from Se yeast. In addition, by conducting a similar experiment in either early (spring experiment 1) or late (autumn experiment 2) lactation, we hypothesized that different Se input-output relationships would result. Both 6-wk experiments involved 60 multiparous Holstein-Friesian cows, all of which had calved in spring. They were allocated to 1 of 10 dietary Se treatments that included 2 types of crushed triticale grain (low Se, approximately 165 microg of Se/kg of DM or high Se, approximately 580 microg/kg of DM) fed at 4 kg of DM/d, and 1 kg of DM/d of pellets formulated to carry 5 quantities of Se yeast (0, 4, 8, 12, or 16 mg of Se). Daily total Se intakes ranged from 18 mg/cow in both experiments. Milk Se concentrations plateaued after 15 and 7 d of supplementation in experiments 1 and 2, respectively, and then remained at plateau concentrations. Average milk Se concentrations for the plateau period increased as the amount of Se yeast increased, and low- and high-Se grain treatments were different at all quantities of Se yeast, although there was a tendency for this difference to diminish at the greatest concentrations of yeast. There were significant positive, linear relationships between Se intake and the concentrations of Se in milk, which were not affected by the source of Se, and the relationships were similar for both experiments. Therefore, the output of Se in milk in experiment 1 was greater than that in experiment 2 because the milk yield of the cows in early lactation was greater. The estimated proportions of Se partitioned to destinations other than milk and feces increased with the amount of Se in the diet and were greater in experiment 2 than in experiment 1, a result that was supported by Se concentrations in whole blood and plasma and in semitendinosus muscle tissue. If high-Se products are to be produced for human nutrition, it is important to be able to develop feeding systems that produce milk with consistent and predictable Se concentrations so that products can consistently meet specifications. The results indicate that this objective is achievable.

Bubbles, Foam Formation, Stability and Consumer Perception of Carbonated Drinks: A Review of Current, New and Emerging Technologies for Rapid Assessment and Control

Publisher: MDPI AG

Date: 20-11-2019

DOI: 10.3390/FOODS8120596

Abstract: Quality control, mainly focused on the assessment of bubble and foam-related parameters, is critical in carbonated beverages, due to their relationship with the chemical components as well as their influence on sensory characteristics such as aroma release, mouthfeel, and perception of tastes and aromas. Consumer assessment and acceptability of carbonated beverages are mainly based on carbonation, foam, and bubbles, as a flat carbonated beverage is usually perceived as low quality. This review focuses on three beverages: beer, sparkling water, and sparkling wine. It explains the characteristics of foam and bubble formation, and the traditional methods, as well as emerging technologies based on robotics and computer vision, to assess bubble and foam-related parameters. Furthermore, it explores the most common methods and the use of advanced techniques using an artificial intelligence approach to assess sensory descriptors both for descriptive analysis and consumers’ acceptability. Emerging technologies, based on the combination of robotics, computer vision, and machine learning as an approach to artificial intelligence, have been developed and applied for the assessment of beer and, to a lesser extent, sparkling wine. This, has the objective of assessing the final products quality using more reliable, accurate, affordable, and less time-consuming methods. However, despite carbonated water being an important product, due to its increasing consumption, more research needs to focus on exploring more efficient, repeatable, and accurate methods to assess carbonation and bubble size, distribution and dynamics.

Wheat bran affects the site of fermentation of resistant starch and luminal indexes related to colon cancer risk: a study in pigs.

Publisher: BMJ

Date: 12-1999

DOI: 10.1136/GUT.45.6.840

Abstract: Recent studies suggest that resistant starch (effective in producing butyrate and lowering possibly toxic ammonia) is rapidly fermented in the proximal colon the distal colon especially would, however, benefit from these properties of resistant starch. To determine whether wheat bran (a rich source of insoluble non-starch polysaccharides), known to hasten gastrointestinal transit, could carry resistant starch through to the distal colon and thus shift its site of fermentation. Twenty four pigs were fed four human type diets: a control diet, or control diet supplemented with resistant starch, wheat bran, or both. Intestinal contents and faeces were collected after two weeks. Without wheat bran, resistant starch was rapidly fermented in the caecum and proximal colon. Supplementation with wheat bran inhibited the caecal fermentation of resistant starch, resulting in an almost twofold increase (from 12.9 (2.5) to 20.5 (2.1) g/day, p<0.05) in resistant starch being fermented between the proximal colon and faeces. This resulted in higher butyrate (133%, p<0.05) and lower ammonia (81%, p<0.05) concentrations in the distal colonic regions. Wheat bran can shift the fermentation of resistant starch further distally, thereby improving the luminal conditions in the distal colonic regions where tumours most commonly occur. Therefore, the combined consumption of resistant starch and insoluble non-starch polysaccharides may contribute to the dietary modulation of colon cancer risk.

Feedlot Factors Influencing the Incidence of Dark Cutting in Australian Grain-Fed Beef

Publisher: MDPI AG

Date: 05-08-2022

DOI: 10.3390/ANI12151989

Abstract: It has been well-established that dark cutting (DC) is a multifactorial issue that is associated with numerous animal and management factors. However, there is limited understanding of the feedlot-based factors that contribute to the influence of DC. The aim of this study was to evaluate the effect of climate, animal, and feedlot factors on the incidence of pH non-compliance in Australian grain-fed cattle. For this study, feedlot and abattoir records from 142,228 in idual cattle over a 1-year period were investigated. These data incorporated records from seven feedlots that consigned cattle to three abattoirs. The average incidence of DC in these carcasses was 2.8%. The production factors that were associated with increased risk of DC included feedlot, sex, hormone growth promotants (HGP), cattle health, and days on feed (DOF). Additionally, DC also increased by reduced solar radiation (SR, W/m2), lower wind speeds (WS, m/s), increased ambient temperature (TA, °C), higher rainfall, a higher average temperature–humidity index (THI), and increased duration of time above heat-load-index threshold of 86 (HLI ≥ 86) during the 7 days prior to feedlot departure. This study identified the feedlot factors that increase the risk of DC from a feedlot-management perspective.

Body Condition Score, Rumination, Intake, Milk Production and Milk Composition of Grazing Dairy Cows Supplemented with Rumen-Protected Lysine and Methionine

Publisher: MDPI AG

Date: 31-08-2021

DOI: 10.3390/DAIRY2030037

Abstract: The study utilised a pasture grazing based, voluntary traffic automatic milking system to investigate milk production of cows fed a pasture-based diet and supplemented with a pellet formulated with vs. without rumen-protected lysine and methionine (RPLM). The study adopted a switch-over design (over two periods of 5 and 10 weeks, respectively) and used 36 cows and equally allocated them into two experimental groups. The RPLM (Trial) pellet had 2% lower crude protein, but similar metabolizable energy content compared to the Control pellet. Pellet intake was 10.0 and 9.4 kg/day/cow. Milk yield was 36.2 and 34.4 kg/day/cow (p = 0.23), and energy corrected milk was 35.1 and 33.8 kg/day/cow (p = 0.076), and milk solids was 2.55 and 2.46 kg/cow/day (p = 0.073) in the Control and Trial groups, respectively. Milk fat%, milk protein%, milk fat: protein ratio, milking frequency and rumination time were not different between the two groups (p 0.05). In period 1, plasma glucose was 3.1 mmol/L for both groups and milk urea were 150 and 127 mg/L in the Control and Trial groups, respectively. Both plasma glucose (as a proxy for energy supply) and milk urea (as a proxy for nitrogen use efficiency NUE) were not different between groups (p 0.05). This study showed that under a grazing pasture system, feeding lactating dairy cows a low protein pellet with RPLM supplementation, maintained milk production performance and NUE, compared with cows fed a high protein Control pellet diet with no RPLM. Further research should assess the long-term (seasonal) effects of feeding a diet formulated with RPLM on cow intake, health and reproductive performance.

Bioactive compounds in microalgae and their potential health benefits

Publisher: Elsevier BV

Date: 10-2022

DOI: 10.1016/J.FBIO.2022.101932

Porcine somatotropin alters body composition and the distribution of fat and lean tissue in the finisher gilt

Publisher: CSIRO Publishing

Date: 2005

DOI: 10.1071/EA04135

Abstract: The present study was designed to determine whether porcine somatotropin (pST) reduces whole animal and belly fat using dual energy X-ray absorptiometry and manual dissection. The study utilised 24 Large White × Landrace gilts selected at 16 weeks of age with an approximate liveweight of 80 kg and housed in in idual pens. Gilts were stratified on liveweight into 8 blocks and 1 pig from each block was assigned to either 0, 5 or 10 mg per day of pST. Pigs were fed ad libitum a wheat-based diet containing 200 g crude protein, 10.2 g available lysine and 14.6 MJ DE/kg, to ensure that responses to pST were expressed. Feed intake and liveweight were measured on a weekly basis. An Hologic QDR4500A dual energy X-ray absorptiometer was used to determine lean, fat and ash composition of pigs initially and again 4 weeks later at the end of the experiment. After slaughter, the composition of the whole half carcass as well as the shoulder, ham, belly and loin primal cuts was determined with dual energy X-ray absorptiometry and verified with manual dissection. Daily pST treatment decreased feed intake (3440, 2710 and 2537 g/day for 0, 5 or 10 mg pST per day, respectively P .001) and decreased feed conversion ratio (2.95, 2.18 and 2.03 g/g P .001) even though there was no significant effect on daily gain. Pigs treated with pST deposited more lean tissue (620, 839 and 873 g/day P .05) and less fat (384, 218 and 176 g/day P .001) than control animals, but there was no effect of pST on ash deposition. As a consequence, pigs treated with 5 and 10 mg pST/day contained 5 and 9 kg less dissectible fat than control gilts, respectively. A dose dependent decrease in belly, loin, ham and shoulder fat was also observed, although the decrease in belly fat was more pronounced than for the whole carcass and other primal cuts. Overall, pST treatment has the potential to decrease carcass and especially belly fat in pigs and increase consumer acceptance of pork in markets that place a premium on carcass fat and lean yield in the belly region. The results determined with dual energy X-ray absorptiometry were confirmed by manual dissection.

The Greater Proportion of Born-Light Progeny from Sows Mated in Summer Contributes to Increased Carcass Fatness Observed in Spring

Publisher: MDPI AG

Date: 10-11-2020

DOI: 10.3390/ANI10112080

Abstract: The backfat of pig carcasses is greater in spring than summer in Australia. The unexplained seasonal variation in carcass backfat creates complications for pig producers in supplying consistent lean carcasses. As a novel explanation, we hypothesised that the increased carcass fatness in spring was due to a greater percentage of born-light progeny from sows that were mated in summer and experienced hot conditions during early gestation. The first part of our experiment compared the birth weight of piglets born to the sows mated in summer (February, the Southern Hemisphere) with those born to sows mated in autumn (May the Southern Hemisphere), and the second part of the experiment compared the growth performance and carcass fatness of the progeny that were stratified as born-light (0.7–1.1 kg) and born-normal (1.3–1.7 kg) from the sows mated in these two seasons. The results showed that the sows mated in summer experienced hotter conditions during early gestation as evidenced by an increased respiration rate and rectal temperature, compared with those mated in autumn. The sows mated in summer had a greater proportion of piglets that were born ≤1.1 kg (24.2% vs. 15.8%, p 0.001), lower average piglet birth weight (1.39 kg vs. 1.52 kg, p 0.001), lower total litter weights (18.9 kg vs. 19.5 kg, p = 0.044) and lower average placental weight (0.26 vs. 0.31 kg, p = 0.011) than those mated in autumn, although litter sizes were similar. Feed intake and growth rate of progeny from 14 weeks of age to slaughter (101 kg live weight) were greater for the born-normal than born-light pigs within the progeny from sows mated in autumn, but there was no difference between the born-light and normal progeny from sows mated in summer, as evidenced by the interaction between piglet birth weight and sow mating season (Both p 0.05). Only the born-light piglets from the sows mated in summer had a greater backfat thickness and loin fat% than the progeny from the sows mated in autumn, as evidenced by a trend of interaction between piglet birth weight and sow mating season (Both p 0.10). In conclusion, the increased proportion of born-light piglets (0.7–1.1 kg range) from the sows mated in summer contributed to the increased carcass fatness observed in spring.

An Extended Photoperiod Increases Milk Yield and Decreases Ovulatory Activity in Dairy Goats

Publisher: MDPI AG

Date: 15-10-2020

DOI: 10.3390/ANI10101879

Abstract: Short day length is associated with reduced milk production in dairy ruminants. Dairy ruminants have been kept in lit sheds during winter to extend the day length and stimulate milk production. However, there studies are few on the effect of an extended photoperiod on the ensuing reproductive performance of dairy goats. The aim of this study was to examine the effect of long day photoperiod (LDPP) and exposure to bucks on milk production and plasma progesterone and prolactin in dairy goats. The study was conducted in 122 non-pregnant lactating dairy goats over 18 weeks from April to August (late autumn and winter in the Southern Hemisphere). The goats were kept in open sided sheds in which the control treatment received ambient lighting while the LDPP treatment received 16 h of light, including artificial lighting. In June, July and August synchronised does were randomly assigned each month to the presence or absence of a buck and ovulatory activity determined from plasma progesterone. Plasma progesterone concentrations were reduced (0.73 vs. 0.46 pmol, p 0.001) while prolactin concentrations were increased (0.095 vs. 1.33 ng/mL, p 0.001) in LDPP goats. The former response was most marked in late winter (0.58 vs. 0.004 pmol, p 0.001) indicating a lack of functional corpora lutea. While there was no overall effect of buck exposure on plasma progesterone concentrations there was a three-way interaction such that plasma progesterone concentrations were increased (p 0.05) by exposure to bucks in LDPP goats in August (late winter) but not at other times. Milk production was increased in LDPP goats over the latter stages of the study (1. 55 vs. 1.82 L/d, p 0.05). Also, persistency of lactation was greater in LDPP goats with fewer goats drying off (13 vs. 0%, p 0.05). These findings suggest that LDPP can increase milk production and persistence while decreasing ovulatory activity in dairy goats.

Mineral and Citrate Concentrations in Milk Are Affected by Seasons, Stage of Lactation and Management Practices

Publisher: MDPI AG

Date: 29-01-2019

DOI: 10.3390/AGRICULTURE9020025

Abstract: This study was conducted to examine associations between nutrition, time of year and season of calving on milk mineral concentrations in 24 pasture-based dairy farms. There was substantial variation in the concentrations (mean with range in parentheses) of Ca 1072 (864–1310) mg/kg citrate 1579 (880–2080) mg/kg P 885 (640–1040) mg/kg Mg 98 (73–122) mg/kg Na 347 (248–554) mg/kg K 1534 (1250–2010) mg/kg and S 295 (155–372) mg/kg with most of the variation associated with stage of lactation, although the influence of days in milk was different for different minerals. Feeding practices were also important in determining the concentrations of some components. Milk Ca, citrate, P, and K concentrations were greater (p 0.05) in cows receiving mineral supplements, while Ca, P and Mg were positively correlated (p 0.05) with the amount of concentrates fed. Milk citrate and K concentrations were positively correlated (p 0.05) with herbage allowance. Milk Se (15 ( –37) µg/kg) and Zn (3.4 (2.2–4.9) mg/kg) also varied with time of year, with Se concentrations also greater (13.7 vs 11.6 µg/kg) in spring compared with autumn calving herds and positively correlated (p 0.05) with the amount of concentrates fed and mineral supplementation. These data indicate that calving pattern and feeding practices could be used to reduce variation in the measured milk mineral concentrations.

The Impact of Plant Phytochemicals on the Gut Microbiota of Humans for a Balanced Life

Publisher: MDPI AG

Date: 23-07-2022

DOI: 10.3390/IJMS23158124

Abstract: The gastrointestinal tract of humans is a complex microbial ecosystem known as gut microbiota. The microbiota is involved in several critical physiological processes such as digestion, absorption, and related physiological functions and plays a crucial role in determining the host’s health. The habitual consumption of specific dietary components can impact beyond their nutritional benefits, altering gut microbiota ersity and function and could manipulate health. Phytochemicals are non-nutrient biologically active plant components that can modify the composition of gut microflora through selective stimulation of proliferation or inhibition of certain microbial communities in the intestine. Plants secrete these components, and they accumulate in the cell wall and cell sap compartments (body) for their development and survival. These compounds have low bioavailability and long time-retention in the intestine due to their poor absorption, resulting in beneficial impacts on gut microbiota population. Feeding diets containing phytochemicals to humans and animals may offer a path to improve the gut microbiome resulting in improved performance and/or health and wellbeing. This review discusses the effects of phytochemicals on the modulation of the gut microbiota environment and the resultant benefits to humans however, the effect of phytochemicals on the gut microbiota of animals is also covered, in brief.

Effects of β-agonist zilpaterol hydrochloride feeding and supplementation period on growth and carcass characteristics of Lori-Bakhtiari lambs

Publisher: Elsevier BV

Date: 06-2014

DOI: 10.1016/J.SMALLRUMRES.2014.01.005

IGFBP-2 inhibits adipogenesis and lipogenesis in human visceral, but not subcutaneous, adipocytes

Publisher: Springer Science and Business Media LLC

Date: 05-11-2014

DOI: 10.1038/IJO.2014.192

Abstract: IGF-binding protein (IGFBP)-2 is the principal IGFBP produced by white adipocytes during adipogenesis, and circulating levels are reduced in obesity. Overexpression of IGFBP-2 in transgenic mice prevents obesity, but depot-specific effects of IGFBP-2 on adipo/lipogenesis are unknown. The present study aimed to investigate whether IGFBP-2 affects adipo/lipogenesis in a depot-specific manner and explore potential mechanisms. Following adipocyte characterisation, IGFBP-2 levels were measured from human subcutaneous and visceral preadipocytes, and IGFBP-2 dose-responses were then undertaken with exogenous IGFBP-2 in an in vitro IGF-I-free system to examine adipo/lipogenesis. Following this, both types of adipocytes were transfected with human siRNA IGFBP-2 to assess auto- ara-/intra-crine effects, with and without additional add-back IGFBP-2. To elucidate the potential mechanisms, visceral preadipocytes were treated with either wild-type or Heparin Binding Domain (HBD)-mutant IGFBP-2 (which is unable to bind to cell-surface components), and experiments were also undertaken using Echistatin (an integrin receptor blocker). Outcomes included gene expression profiles, protein levels and phosphorylation and lipid staining. Human visceral adipocytes produced significantly more IGFBP-2 than subcutaneous adipocytes. Subsequent dose-responses to IGFBP-2 demonstrated significant reductions in adipo/lipogenesis in visceral, but not subcutaneous, adipocytes in response to increasing IGFBP-2. Silencing IGFBP-2 resulted in exaggerated adipo/lipogenesis in visceral, but not subcutaneous, adipocytes, an effect completely inhibited by add-back IGFBP-2. These effects occurred in the absence of changes in IGF-I levels. HBD-mutant IGFBP-2 had reduced effects compared with wild-type IGFBP-2. Wild-type IGFBP-2 increased phosphorylation of focal adhesion kinase (FAK) and decreased phosphatase and tensin homolog (PTEN) levels, suggestive of integrin-mediated signalling. Blockade of this signalling, using Echistatin, completely negated the effects of IGFBP-2 on visceral adipo/lipogenesis. IGFBP-2 inhibits both adipogenesis and lipogenesis in visceral, but not subcutaneous, adipocytes. This depot-specific impairment appears to be independent of IGF-I and involves cell-surface association of IGFBP-2 and activation of integrin signalling pathways.

Modelling and Validation of Computer Vision Techniques to Assess Heart Rate, Eye Temperature, Ear-Base Temperature and Respiration Rate in Cattle

Publisher: MDPI AG

Date: 06-12-2019

DOI: 10.3390/ANI9121089

Abstract: Precision livestock farming has emerged with the aim of providing detailed information to detect and reduce problems related to animal management. This study aimed to develop and validate computer vision techniques to track required features of cattle face and to remotely assess eye temperature, ear-base temperature, respiration rate, and heart rate in cattle. Ten dairy cows were recorded during six handling procedures across two consecutive days using thermal infrared cameras and RGB (red, green, blue) video cameras. Simultaneously, core body temperature, respiration rate and heart rate were measured using more conventional ‘invasive’ methods to be compared with the data obtained with the proposed algorithms. The feature tracking algorithm, developed to improve image processing, showed an accuracy between 92% and 95% when tracking different areas of the face of cows. The results of this study also show correlation coefficients up to 0.99 between temperature measures obtained invasively and those obtained remotely, with the highest values achieved when the analysis was performed within in idual cows. In the case of respiration rate, a positive correlation (r = 0.87) was found between visual observations and the analysis of non-radiometric infrared videos. Low to high correlation coefficients were found between the heart rates (0.09–0.99) obtained from attached monitors and from the proposed method. Furthermore, camera location and the area analysed appear to have a relevant impact on the performance of the proposed techniques. This study shows positive outcomes from the proposed computer vision techniques when measuring physiological parameters. Further research is needed to automate and improve these techniques to measure physiological changes in farm animals considering their in idual characteristics.

Functionality and genomics of selenium and vitamin E supplementation in ruminants

Publisher: CSIRO Publishing

Date: 2016

DOI: 10.1071/AN15263

Abstract: Selenium (Se) and vitamin E are essential micronutrients for animal health and production. The major function of both Se and vitamin E is to prevent the oxidative damage of biological membranes and they can influence growth, reproduction, immune function, health, and product quality in ruminants. Both Se and vitamin E are important for maintaining low cellular and systemic concentrations of reactive oxygen species and lipid hydroperoxides, to ensure optimum cellular function. Discovery of various selenoproteins and vitamin E-responsive genes has contributed significantly to improving our understanding about multiple functions of Se and vitamin E. There is evidence that these functions extend beyond the classical antioxidant properties to immunomodulation and intracellular cell signalling and gene regulation. Research in recent years has also shown that supranutritional supplementation of Se and vitamin E is required to improve the performance of ruminants under certain stressful conditions such as heat stress and during transition period. Considering the growing awareness among consumers of the benefits of antioxidant-rich food, there is a great opportunity for the livestock industries to focus on producing antioxidant-enriched milk and meat products or functional foods. The present review focuses on the recent developments in understanding multiple functions of Se and vitamin E at the cellular and molecular level and the effects of supranutritional supplementation on ruminant performance. In addition, the paper also articulates the potential opportunities to produce functional foods enriched with antioxidants, and underlines the need for optimum supplementation of these micronutrients for efficient ruminant production.

Effects of dietary fat and conjugated linoleic acid on plasma metabolite concentrations and metabolic responses to homeostatic signals in pigs

Publisher: Cambridge University Press (CUP)

Date: 12-2002

DOI: 10.1079/BJN2002726

Abstract: Sixteen female cross-bred (Large White × Landrace) pigs (initial weight 65 kg) with venous catheters were randomly allocated to four treatment groups in a 2×2 factorial design. The respective factors were dietary fat (25 or 100 g/kg) and dietary conjugated linoleic acid (CLA 0 or 10 g CLA-55/kg). Pigs were fed every 3 h (close to ad libitum digestible energy intake) for 8 d and were bled frequently. Plasma glucose and non-esterified fatty acid (NEFA) responses to insulin and adrenaline challenges were determined on day 8. Plasma concentrations of NEFA were significantly increased (10·5 and 5·4 % for low- and high-fat diets respectively, P =0·015) throughout the experiment, suggesting that there was a possible increase in fat mobilisation. The increase in lipolysis, an indicator of ß-adrenergic stimulated lipolysis, was also evident in the NEFA response to adrenaline. However, the increase in plasma triacylglycerol (11·0 and 7·1 % for low- and high-fat diets respectively, P =0·008) indicated that CLA could have reduced fat accretion via decreased adipose tissue triacylglycerol synthesis from preformed fatty acids, possibly through reduced lipoprotein lipase activity. Plasma glucose, the primary substrate for de novo lipid synthesis, and plasma insulin levels were unaffected by dietary CLA suggesting that de novo lipid synthesis was largely unaffected ( P =0·24 and P =0·30 respectively). In addition, the dietary CLA had no effect upon the ability of insulin to stimulate glucose removal.

Acute exercise stress and electrical stimulation influence the consumer perception of sheep meat eating quality and objective quality traits

Publisher: CSIRO Publishing

Date: 2005

DOI: 10.1071/EA03270

Abstract: The effects of acute exercise stress and electrical stimulation on lamb eating quality and objective measurements of quality were evaluated on the Longissimus thoracis et lumborum (LTL) and the Gluteus medius (GM) muscles. Meat quality attributes were also assessed on the LTL, Semimembranosus (SM) and the Semispinalis capitis (SC) muscles. Forty-eight Poll Dorset × Border Leicester–Merino ewes of about 6 months old were randomly allocated to treatments in a 2 × 2 factorial design, with the respective factors being exercise (Ex, 0 v. 15 min) and post-slaughter low voltage electrical stimulation (control v. ES). In general, exercise reduced the rate of pH fall and increased the ultimate pH of all 3 muscles (P .05). This effect was more pronounced in the SM than in the LTL and SC. Exercised LTL and SM muscles also had lower surface lightness (L*) values and higher muscle total water content, indicating an increased incidence of dark cutting meat. Exercised LTL steaks were rated higher for tenderness and juiciness by the taste panelists and had lower Warner-Bratzler shear force values, as is often observed with high ultimate pH meat. Electrical stimulation reduced initial pH (pH0.5) values, but resulted in a slower rate of pH fall in the LTL and SM thereafter. Electrical stimulation resulted in an elevation of temperature at rigor in the SM by 10°C (15 v. 25°C) and in the LTL by 3.1°C (7.4 v. 10.5°C). While no effect of electrical stimulation was observed with instrumental analysis, electrical stimulation reduced sensory tenderness scores and overall liking for the GM, and tended to reduce flavour and juiciness of both the GM and LTL. In conclusion, acute exercise pre-slaughter improved eating quality, but caused dark cutting meat. The negative effect of low voltage stimulation on the eating quality of the GM was probably due to a high rigor temperature and associated heat toughening.

Producing milk with uniform high selenium concentrations on commercial dairy farms

Publisher: CSIRO Publishing

Date: 2011

DOI: 10.1071/AN10133

Abstract: Six herds on five commercial dairy farms were involved in the production of high selenium (Se) milk. The farms had a range of herd sizes, herd structures, feeding systems and milk production per cow. On all farms, pelleted concentrate supplements containing Se yeast were fed twice daily in the dairy for 16 days. The objectives were to: (1) produce milk with Se concentrations exceeding 225 μg/kg on the five farms for pilot-scale production of a high protein milk powder (2) validate a predictive relationship between Se intake and milk Se concentration developed in research and (3) examine the time taken from the introduction of Se yeast to steady-state concentrations of Se in milk under a range of commercial farming conditions. We hypothesised that the relationship between Se intake and its concentration in milk found in research would apply on commercial farms. Daily Se intake, which was primarily from Se yeast in the pelleted concentrates, varied from 35 to 51 mg Se/cow. Grazed pasture and conserved forage contributed less than 1 mg Se/cow on all farms. The time taken from the introduction of pellets containing Se yeast to steady-state milk Se concentrations was 4–7 days. The steady-state Se concentrations in milk varied from 166 to 247 µg/kg, but these concentrations were only 55–72% of predicted values. All the milk produced from the five farms on the last 2 days of feeding of Se-enriched pellets was used to produce a milk protein concentrate with a Se concentration of 5.4 mg/kg. Factors that might have affected Se incorporation into milk and the implications of these results for commercial production of high Se milk or milk products are discussed.

Consumption of brown onions (Allium cepa var. cavalier and var. destiny) moderately modulates blood lipids, haematological and haemostatic variables in healthy pigs

Publisher: Cambridge University Press (CUP)

Date: 02-2004

DOI: 10.1079/BJN20031036

Abstract: Although garlic and onions have long been associated with putative cardiovascular health benefits, the effects of different commercially available onions and level of intake have not been studied. Therefore, the aim of the present study was to evaluate the potential health benefits of raw onions using the pig as a biomedical model. Twenty-five female (Large White×Landrace) pigs were used in a (2×2)+1 factorial experiment. Pigs were fed a standard grower diet supplemented with 100 g tallow/kg with the addition of Allium cepa var. cavalier or var. destiny at 0, 10 or 25 g/MJ digestible energy for 6 weeks. Overall, the consumption of onions resulted in significant reductions in plasma triacylglycerol however, the reductions were most pronounced in pigs fed destiny onions (−26 %, P =0·042). Total plasma cholesterol and LDL:HDL ratios were not significantly different. Onion supplementation, regardless of the variety, resulted in dose-dependent reductions in erythrocyte counts and Hb levels, while the white blood cell concentrations, particularly lymphocytes, were increased in pigs that consumed onions. Furthermore, indices of blood clotting were largely unaffected by onion consumption. In conclusion, dietary supplementation with raw brown onions has moderate lipid-modulating and immunostimulatory properties. However, daily onion intake g/MJ digestible energy could be detrimental to erythrocyte numbers.

Association of Phenotypic Markers of Heat Tolerance with Australian Genomic Estimated Breeding Values and Dairy Cattle Selection Indices

Publisher: MDPI AG

Date: 16-05-2023

DOI: 10.20944/PREPRINTS202305.1076.V1

Abstract: Dairy cattle predicted by genomic breeding values to be heat tolerant are known to have less milk production decline and lower core body temperature increases in response to elevated temperatures. In a study conducted at the University of Melbourne’s Dookie Robotic Dairy Farm during summer, we identified the most 20 heat-susceptible and 20 heat-tolerant cows in a herd of 150 Holstein Friesian lactating cows based on their phenotypic responses (changes in respiration rate, surface body temperature, panting score, and milk production). Hair s les were collected from the tip of the cows& #039 tail following standard genotyping protocols. The results indicated variation in feed saved and HT genomic estimated breeding values (GEBVs) (P≤0.05) across age, indicating a potential for their selection. As expected, the thermotolerant group had higher GEBVs for HT and feed saved but lower for milk production. In general, younger cows had superior GEBVs for Balanced Performance Index (BPI), Type Weighted Index (TWI) and Australian Selection Index (ASI), whilst older cows were superior in fertility, feed saved (FS) and HT. The study demonstrated highly significant (P≤ 0.001) negative correlations (-0.28 to -0.74) between HT and GEBVs for current Australian Dairy cattle selection indices (BPI, TWI, ASI, HWI) and significant (P≤ 0.05) positive correlations between HT and GEBVs for traits like FS (0.45) and fertility (0.25). Genomic selection for HT will help improve cow efficiency and sustainability of dairy production under hot summer conditions. However, a more extensive study involving more lactating cows across multiple farms is recommended to confirm the associations between the phenotypic predictors of HT and GEBVs.

Long [R3] insulin-like growth factor-I reduces growth, plasma growth hormone, IGF binding protein-3 and endogenous IGF-I concentrations in pigs.

Publisher: Bioscientifica

Date: 12-1997

DOI: 10.1677/JOE.0.1550559

Abstract: Growth hormone (GH) improves growth performance in the pig. Analogues of insulin-like growth factor-I (IGF-I) that bind poorly to IGF binding proteins (IGFBP) stimulate growth in the rat but, in contrast, inhibit growth in the pig. This study was designed to determine the effect of IGF peptides alone or in combination with porcine GH (pGH) on growth characteristics and plasma hormone concentrations in finisher pigs. A four-day infusion of Long [R3] IGF-I (LR3IGF-I 180 micrograms/kg/day) decreased the average daily gain, food intake, and plasma IGFBP-3, IGF-I and insulin concentrations. The mean plasma GH concentration was decreased by 23% and the area under the GH peaks was reduced by 60%. Co-administration of pGH (30 micrograms/kg/day) with LR3IGF-I had no interactive effect on growth performance, and plasma insulin, IGFBP-3 and IGF-I concentrations remained suppressed. The area under the GH peaks was not restored with this combination treatment although mean plasma GH concentrations were elevated in all animals receiving pGH. Infusion of IGF-I (180 micrograms/kg/day) decreased plasma insulin and mean GH concentrations but had no significant effect on IGFBP-3 concentrations. Average daily gain and feed intake were not changed by IGF-I treatment. A combination of IGF-I and pGH injection (30 micrograms/kg/day) increased plasma IGFBP-3 concentrations but plasma insulin levels remained suppressed. Plasma glucose levels were unaffected by any treatment. The study demonstrates that both IGF-I and LR3IGF-I suppress plasma GH concentrations in finisher pigs. This, in turn, may be responsible for the reduction in the plasma concentration of IGF-I, IGFBP-3 and insulin seen in LR3IGF-I-treated animals. The decrease in these parameters may contribute to the inhibitory effect of LR3IGF-I on growth performance in the pig.

Differences in Hedonic Responses, Facial Expressions and Self-Reported Emotions of Consumers Using Commercial Yogurts: A Cross-Cultural Study

Publisher: MDPI AG

Date: 29-05-2021

DOI: 10.3390/FOODS10061237

Abstract: Hedonic scale testing is a well-accepted methodology for assessing consumer perceptions but is compromised by variation in voluntary responses between cultures. Check-all-that-apply (CATA) methods using emotion terms or emojis and facial expression recognition (FER) are emerging as more powerful tools for consumer sensory testing as they may offer improved assessment of voluntary and involuntary responses, respectively. Therefore, this experiment compared traditional hedonic scale responses for overall liking to (1) CATA emotions, (2) CATA emojis and (3) FER. The experiment measured voluntary and involuntary responses from 62 participants of Asian (53%) versus Western (47%) origin, who consumed six ergent yogurt formulations (Greek, drinkable, soy, coconut, berry, cookies). The hedonic scales could discriminate between yogurt formulations but could not distinguish between responses across the cultural groups. Aversive responses to formulations were the easiest to characterize for all methods the hedonic scale was the only method that could not characterize differences in cultural preferences, with CATA emojis displaying the highest level of discrimination. In conclusion, CATA methods, particularly the use of emojis, showed improved characterization of cross-cultural preferences of yogurt formulations compared to hedonic scales and FER.

Heat Stress and Histopathological Changes of Vital Organs: A Novel Approach to Assess Climate Resilience in Farm Animals

Publisher: MDPI AG

Date: 09-01-2023

DOI: 10.3390/SU15021242

Abstract: Heat stress causes functional and metabolic alterations in different cells and tissues. There are several pathomorphological changes and biomarkers associated with head load in adaptive and productive organs of livestock. Heat stress-induced histopathological alterations in livestock were categorized as degenerative changes (fatty degeneration, steatosis, hydropic degeneration), necrosis (pyknosis, fibrosis), circulatory disturbances (hyperemia, edema, hemorrhage, congestion, thrombosis, ischemia), growth disturbances (hyperplasia, atrophy) and focal/diffuse inflammation (vascular changes, exudation). Upon immunohistochemical analysis, the biomarkers identified in growth-related organs were HSP70, HSP60, GABA, GABAAR, GABABR, HSP90, GnRH, LH, FSH, m6A, Nrf2, and C/EBPβ. The biomarkers in the reproductive organs were HSP70, Bax, Bcl-2, GABA, GABAAR, GABABR, Caspase-3, HSP90, HSPB9, HSPB10, HSF1, HSP40, T, E2, Cyt-C, CAT, BCL2L1, and VEGF. The identified biomarkers in the immune organs were CD3+ T cells, CD4+ T cells, CD8+ T cells, HSP70, and Bcl-2. All these biomarkers could serve as reliable variables in heat stress assessment in livestock. Further, HSP70, HSP90, HSP60, NPY, HSP27, Bcl-2, NF-κB, AQP2, Insulin, CD3+ T cells, CD4+ T cells, CD172a, EGF, AQP1, AQP3, AQP4, AQP5, CRYAB, GHR, 5-HT, CCK, and GLP-1 are heat stress-related biomarkers in adaptive organs that help in assessing the climate resilience of a livestock species and improving understanding about adaptive mechanisms. Among these biomarkers, HSP70 was established to be the ideal cellular biomarker for scaling heat response in livestock. Thus, examining heat-stressed organ histopathology and identifying cellular markers by immunohistochemistry may lay the foundation for screening climate-resilient livestock breeds in the challenging climatic scenario. Further, such an approach could help in developing concepts to combat the detrimental consequences of heat stress to ensure sustainability in livestock production.

LC-ESI-QTOF/MS characterisation of phenolic acids and flavonoids in polyphenol-rich fruits and vegetables and their potential antioxidant activities

Publisher: MDPI AG

Date: 17-09-2019

DOI: 10.3390/ANTIOX8090405

Abstract: Polyphenols are naturally occurring compounds found largely in fruits and vegetables. The antioxidant properties of these polyphenols including total phenolic content (TPC), total flavonoid content (TFC), tannin content, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH), 2,2′-azinobis-(3-ethylbenzo-thiazoline-6-sulfonic acid) (ABTS) scavenging abilities and ferric ion reducing antioxidant power (FRAP) were measured among sixteen (16) plant foods (mango, blueberry, strawberry, black carrot, raspberry, dark grapes, garlic, ginger, onion, cherry, plum, apple, papaya, peach, pear and apricot) by modifying, standardising and translating existing antioxidant methods using a 96-well plate reader. Eighteen targeted phenolic acids and flavonoids were characterised and quantified using high-performance liquid chromatography-photometric diode array (HPLC-PDA) and verified by modifying an existing method of liquid chromatography coupled with electrospray-ionisation triple quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF/MS). While most of these compounds were accurately detected by the HPLC-PDA at a low concentration, a few polyphenols in low concentrations could be only be characterised using the LC-ESI-QTOF/MS method. Our results showed that mango possessed the highest overall antioxidant activity, phenolic acid and flavonoid content among the selected fruits. Factor analysis (FA) and Pearson’s correlation tests showed high correlations among ABTS, DPPH, FRAP and phenolic acids, implying the comparable capabilities of scavenging the DPPH/ABTS free radicals and reducing ferric ions from the antioxidant compounds in the s les. Phenolic acids contributed significantly to the antioxidant activities, and flavonoids contributed more to tannin content based on the correlations. Overall, methods modified and standardized in this study can provide better understanding of high throughput technologies and increase the reliability of antioxidant data of different plant foods.

Betaine and Isoquinoline Alkaloids Protect against Heat Stress and Colonic Permeability in Growing Pigs

Publisher: MDPI AG

Date: 21-10-2020

DOI: 10.3390/ANTIOX9101024

Abstract: Heat stress (HS) compromises productivity of pork production, in part as a result of increased oxidative stress and inflammatory responses, particularly within the gastrointestinal tract. This study aimed to investigate whether plant-derived betaine and isoquinoline alkaloids could ameliorate HS in pigs. Fifty female Large White × Landrace grower pigs, which were acclimated to control (CON), control plus betaine (BET), or control plus isoquinoline alkaloids (IQA) diets for 14 days were then exposed to heat stress or thermoneutral condition. Both BET and IQA partially ameliorated increases in respiration rate (p = 0.013) and rectal temperature (p = 0.001) associated with HS conditions. Heat stress increased salivary cortisol concentrations and reduced plasma creatinine, lactate, and thyroid hormone concentrations. Heat stress increased colon FD4 permeability, which was reduced by IQA (p = 0.030). Heat stress increased inflammation in the jejunum and ileum, as indicated by elevated interleukin-1β (p = 0.022) in the jejunum and interleukin-1β (p = 0.004) and interleukin-8 (p = 0.001) in the ileum. No differences in plasma total antioxidant capacity (TAC) were observed with HS, but betaine increased plasma TAC compared to IQA. Dietary BET increased betaine concentrations in the jejunum, ileum (p 0.001 for both), plasma, liver, kidney (p 0.010 for all), urine (p = 0.002) and tended to be higher in muscle (p = 0.084). Betaine concentration was not influenced by HS, but it tended to be higher in plasma and accumulated in the liver. These data suggest that betaine and isoquinoline alkaloids supplementation ameliorated consequences of heat stress in grower pigs and protected against HS induced increases in colonic permeability.

LC-ESI/QTOF-MS Profiling of Chicory and Lucerne Polyphenols and Their Antioxidant Activities

Publisher: MDPI AG

Date: 08-06-2021

DOI: 10.3390/ANTIOX10060932

Abstract: Chicory and lucerne are used as specialised forages in sheep or dairy production systems in some parts of the world. Recently, these plants are gaining attention as raw materials in the search for natural antioxidants for use in animal feeds, human foods and nutraceutical formulations. The antioxidant potential of these plants is credited to polyphenols, a subgroup of phytochemicals. Therefore, phenolic characterisation is an essential step before their use as ingredients in animal feeds, human food or nutraceutical preparations. In this study, we performed qualitative and quantitative analysis of polyphenols in chicory and lucerne. Profiling of polyphenols from chicory and lucerne was performed by LC-ESI/QTOF-MS with a total of 80 phenolic compounds identified in chicory and lucerne. The quantification of polyphenols was achieved by high performance liquid chromatography, coupled with a photo diode array (HPLC-PDA). Chicoric acid was the major phenolic acid found in chicory, with the highest concentration (1692.33 ± 0.04 µg/g DW) among all the polyphenols quantified in this study. 2-hydroxybenzoic acid was the major phenolic acid found in lucerne, with the highest concentration of 1440.64 ± 0.04 µg/g DW. Total phenolic, flavonoids and total tannin contents were measured, and the antioxidant potential was determined by 2,2-Diphenyl-1-picrylhydrazyl, Ferric Reducing Antioxidant Power, 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic Acid, Hydroxyl (OH−) Radical Scavenging Activity, Chelating Ability of Ferrous Ion (Fe2+) and Reducing Power (RPA) assays. Both chicory (8.04 ± 0.33 mg AAE/g DW) and lucerne (11.29 ± 0.25 mg AAE/g DW) showed high values for Hydroxyl (OH−) Radical Scavenging Activity. The current study allowed us to draw a profile of polyphenols from chicory and lucerne. They provided a molecular fingerprint useful for the application of these plant materials in human foods, animal feeds and pharmaceutical formulations.

Dietary lecithin improves feed efficiency without impacting meat quality in immunocastrated male pigs and gilts fed a summer ration containing added fat

Publisher: Elsevier BV

Date: 06-2018

DOI: 10.1016/J.ANINU.2018.01.008

The β3-adrenergic agonist (BRL35135A) improves feed efficiency and decreases visceral but not subcutaneous fat in lambs

Publisher: Elsevier BV

Date: 2013

DOI: 10.1016/J.SMALLRUMRES.2012.07.028

The effects of immuno- and surgical-castration on the behaviour and consequently growth of group-housed, male finisher pigs

Publisher: Elsevier BV

Date: 04-2003

DOI: 10.1016/S0168-1591(02)00256-3

Non-contact heart rate and blood pressure estimations from video analysis and machine learning modelling applied to food sensory responses: A case study for chocolate

Publisher: MDPI AG

Date: 03-06-2018

DOI: 10.3390/S18061802

Effect of processing on polyphenols in butternut pumpkin (Cucurbita moschata)

Publisher: Elsevier BV

Date: 10-2022

DOI: 10.1016/J.FBIO.2022.101925

Influence of different systems for feeding supplements to grazing dairy cows on milk fatty acid composition

Publisher: Cambridge University Press (CUP)

Date: 24-02-2014

DOI: 10.1017/S002202991400003X

Abstract: This study investigated the effects of different strategies for feeding supplements to grazing dairy cows on the proportions of fatty acids in milk. Two hundred and sixteen cows were fed supplementary grain and forage according to one of 3 different strategies (1) Control: cows grazed perennial ryegrass pasture (14 kg dry matter/d) supplemented with milled barley grain fed in the milking parlour and pasture silage offered in the paddock (2) Partial mixed ration 1 (PMR1): same pasture allotment and supplement as Control strategy, but the supplements presented as a mixed ration after each milking in feedpad, and (3) Partial mixed ration 2 (PMR2): same pasture allotment, supplemented with a mixed ration of milled barley grain, alfalfa hay, corn silage and crushed corn grain fed in a feedpad. Within each strategy, cows were assigned to receive either 6, 8, 10 or 12 kg dry matter supplement/cow per d. Milk fatty acid proportions from cows fed Control and PMR1 strategies were similar and different from those fed PMR2, particularly at 10 to 12 kg dry matter supplement/cow per d. The reduction in milk fat yield and concentration in cows fed high amounts of supplement as Control and PMR1 was coincident with 4 × increase in 10 t- 18:1 proportion. The composition of the partial mixed ration (PMR) and the amount offered affected milk fatty acid proportions and milk fat content, however, the method of supplementation did not.

Bioaccessibility and bioactivities of phenolic compounds from roasted coffee beans during in vitro digestion and colonic fermentation

Publisher: Elsevier BV

Date: 08-2022

DOI: 10.1016/J.FOODCHEM.2022.132794

Abstract: Bioaccessibility and bioactivity of phenolic compounds in coffee beans relate to roasting and digestion process. This study aimed to estimate phenolic content, antioxidant potential, bioaccessibility, and changes in short chain fatty acids (SCFAs) production during in vitro digestion and colonic fermentation of commercial roasted (light, medium and dark) coffee beans. There was no significant difference found among all three different roasting levels. TPC and DPPH were enhanced 15 mg GAE/g and 60 mg TE/g during gastrointestinal digestion, respectively. For colonic fermentation, the highest TPC and FRAP of all coffee beans was found at 2 and 4 h, respectively. The gastric bioaccessibility of most of the phenolic compounds were relatively higher due to thermal phenolic degradation. Total SCFAs production was only up to 0.02 mM because of thermal polysaccharide decomposition. Light roasted beans exhibited relatively higher phenolic bioaccessibility, antioxidant activities and SCFAs production, which would be more beneficial to gut health.

Porcine somatotropin (pST) administered using a commercial delivery system improves growth performance of rapidly growing, group-housed finisher pigs

Publisher: CSIRO Publishing

Date: 2002

DOI: 10.1071/AR01013

Abstract: Porcine somatotropin (pST) treatment of pigs consistently improves average daily gain (ADG) and feed conversion ratio (FCR). However, most studies have been conducted with in idually housed pigs, of unimproved genotype and over a lighter finisher phase than current practice. The present study was designed to determine whether a commercial pST (Reporcin) treatment regime would improve growth performance in heavy boars and gilts (initial weight 79 kg) of an improved genotype and housed under commercial conditions. The 2 2 factorial experiment involved 160, 19-week-old Large White Landrace pigs (80 males and 80 females) in 20 pens of 8 pigs en. The respective factors were sex (boars and gilts) and dose of pST (0 and 5 mg/day). Pigs were fed a wheat-based diet formulated to contain 200 g crude protein, 10.2 g available lysine, and 14.6 MJ DE/kg to ensure that responses to pST and sex were expressed. Injections of pST were given daily for 28 days prior to slaughter using a commercial applicator designed for this purpose. Pigs were bled by venipuncture on days 7 and 28 of treatment and the plasma s les analysed for plasma urea nitrogen (PUN) and glucose. The degree of stomach ulceration at slaughter was assessed by a veterinary pathologist. Daily pST treatment increased ADG (P = 0.003), particularly in gilts, as indicated by the interaction (P = 0.015) between sex and pST. Thus, gilts treated with pST grew 23% faster than control gilts (1093 v. 1273 g/day), whereas the pST-treated boars grew only slightly faster (+2.5%) than control boars (1261 v. 1291 g/day). Feed intake was similar for boars and gilts and was decreased in both sexes by 10% during pST treatment. FCR was higher in control gilts than in boars and was improved by pST treatment. This was particularly evident in the gilts such that there was no difference in the FCR of pST-treated gilts and boars. Back fat at slaughter was reduced by 2.3 and 3.2 mm in boars and gilts treated with pST, respectively. The only stomach lesions observed were very minor in severity and there was no effect of pST on the proportion of pigs exhibiting stomach ulcers (7/80 and 9/79 for control and pST-treated pigs, respectively χ2 = 0.31, P = 0.58) or visible possible injection site lesions (0/80 and 1/79 χ2 = 1.01, P = 0.31). The PUN response mirrored the effects of sex and pST on FCR. Indeed, there was a high correlation (R = +0.84, P 0.001) between FCR and PUN. In conclusion, pST treatment of finisher pigs of an improved genotype and housed under simulated commercial conditions improved growth performance, decreased back fat, and negated sex differences.

LC-ESI-QTOF-MS/MS Characterization of Seaweed Phenolics and Their Antioxidant Potential

Publisher: MDPI AG

Date: 24-06-2020

DOI: 10.3390/MD18060331

Abstract: Seaweed is an important food widely consumed in Asian countries. Seaweed has a erse array of bioactive compounds, including dietary fiber, carbohydrate, protein, fatty acid, minerals and polyphenols, which contribute to the health benefits and commercial value of seaweed. Nevertheless, detailed information on polyphenol content in seaweeds is still limited. Therefore, the present work aimed to investigate the phenolic compounds present in eight seaweeds [Chlorophyta (green), Ulva sp., Caulerpa sp. and Codium sp. Rhodophyta (red), Dasya sp., Grateloupia sp. and Centroceras sp. Ochrophyta (brown), Ecklonia sp., Sargassum sp.], using liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QTOF-MS/MS). The total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC) were determined. The antioxidant potential of seaweed was assessed using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay, a 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging assay and a ferric reducing antioxidant power (FRAP) assay. Brown seaweed species showed the highest total polyphenol content, which correlated with the highest antioxidant potential. The LC-ESI-QTOF-MS/MS tentatively identified a total of 54 phenolic compounds present in the eight seaweeds. The largest number of phenolic compounds were present in Centroceras sp. followed by Ecklonia sp. and Caulerpa sp. Using high-performance liquid chromatography-photodiode array (HPLC-PDA) quantification, the most abundant phenolic compound was p-hydroxybenzoic acid, present in Ulva sp. at 846.083 ± 0.02 μg/g fresh weight. The results obtained indicate the importance of seaweed as a promising source of polyphenols with antioxidant properties, consistent with the health potential of seaweed in food, pharmaceutical and nutraceutical applications.

Dietary lecithin improves dressing percentage and decreases chewiness in the longissimus muscle in finisher gilts

Publisher: Elsevier BV

Date: 03-2014

DOI: 10.1016/J.MEATSCI.2013.10.028

Abstract: The influence of dietary lecithin at doses of 0, 4, 20 or 80 g/kg fed to finisher gilts for six weeks prior to slaughter on growth performance, carcass quality and pork quality was investigated. M. longissimus lumborum (loin) was removed from 36 pig carcasses at 24h post-mortem for Warner-Bratzler shear force, compression, collagen content and colour analyses. Dietary lecithin increased dressing percentage (P=0.009). Pork chewiness and collagen content were decreased by dietary lecithin (P 0.05, respectively). Dietary lecithin reduced loin muscle L* values and increased a* values (P<0.05, respectively) but no changes on b* values (P=0.56). The data showed that dietary lecithin improved dressing percentage and resulted in less chewy and less pale pork.

Dietary ractopamine promotes growth, feed efficiency and carcass responses over a wide range of available lysine levels in finisher boars and gilts

Publisher: CSIRO Publishing

Date: 2013

DOI: 10.1071/AN11351

Abstract: The aim of this study was to investigate the performance and carcass responses of finisher boars and gilts offered a range of dietary lysine levels and three levels of dietary ractopamine hydrochloride (RAC). The study involved three experiments of 90 pigs each, totalling 270 in idually penned pigs in a 2 by 5 by 3 factorial design comprising two sexes (gilts, boars), five levels of dietary lysine [0.40, 0.48, 0.56, 0.64, and 0.72 g available lysine per MJ of digestible energy (DE), respectively] and three RAC dose regimes (0, 5 and 10 mg/kg) for 28 days. An outbreak of pneumonia (Actinobaccilus pleuropneumonia) at Day 26 in Experiment 1 compromised Day 28 data however, Day 21 data was considered suitable across all three experiments. The results indicate that 0.56 g available lysine/MJ DE is sufficient to maximise average daily gain (ADG), feed conversion ratio (FCR) and carcass weight in gilts. Control boars indicated that ADG and FCR were not limited by the lysine : energy ratios fed in this study. Increasing levels of dietary lysine linearly increased ADG (P 0.001), improved FCR (P 0.001) and increased carcass weight (P = 0.001). Likewise, increasing dietary RAC further improved ADG (P = 0.001), FCR (P = 0.002) and carcass weight (P = 0.075) linearly. The critical lysine levels calculated for ADG and FCR in gilts fed diets supplemented with RAC were less than required for controls. Boars had higher critical lysine levels than gilts when supplemented with dietary RAC, and increasing dietary RAC increased critical lysine levels for ADG and FCR in gilts and boars. An interaction (P = 0.016) between dietary lysine and RAC occurred for FCR, such that the response to 5 mg/kg dietary RAC diminished in diets containing 0.64 g and 0.72 g available lysine/MJ DE however, these diets elicited a response when supplemented with 10 mg/kg RAC. Responses in ADG, FCR and carcass weight to dietary RAC were noted when dietary lysine was at or below the current recommendations for RAC diets, and it was suggested that this may have been due to reduced efficiency of lysine utilisation due to chronic disease challenge. A Sex × RAC interaction (P = 0.027) occurred for carcass P2, indicating the higher RAC dose reduced carcass P2 in boars but not in gilts. When formulating finisher pig diets between 60 and 90 kg liveweight, consideration of the lysine : energy requirements for boars and gilts is needed in order to maximise ADG, FCR and carcass characteristics. When supplementing pigs with dietary RAC, a wide range of lysine : energy levels maybe employed however, this is dependent on RAC inclusion level and probably herd health.

Effects of infusing nitric oxide donors and inhibitors on plasma metabolites, muscle lactate production and meat quality in lambs fed a high quality roughage-based diet

Publisher: Elsevier BV

Date: 07-2015

DOI: 10.1016/J.MEATSCI.2015.02.007

Abstract: As nitric oxide (NO) is postulated to be a mediator of the effects of pre-slaughter stress on meat quality the aims of this experiment were to investigate the effects of modulating NO pharmacologically on meat quality of sedentary lambs. As pharmacological NO donors are prohibitively expensive to use in the lamb model L-Arginine, the substrate for NO synthase (NOS) was infused into lambs and increased NO production by ~30%. In a 2 × 2 factorial design we infused either L-Arginine (500 mg/kg) or the NOS inhibitor L-N(G) nitroarginine methyl ester hydrochloride (L-NAME, 30 mg/kg) 190 min pre-slaughter and investigated meat quality in the Longissimus thoracis lumborum (LTL) or Semimembranosus (SM). The principal outcome of the experiment was that L-NAME inhibited proteolysis and reduced tenderness in the SM. These data indicate that events pre-slaughter that affect NO synthesis can influence meat tenderness, potentially via altered muscle metabolism or modulation of proteolytic enzymes.

LC-ESI-QTOF-MS/MS Characterization of Phenolic Compounds in Common Commercial Mushrooms and Their Potential Antioxidant Activities

Publisher: MDPI AG

Date: 03-06-2023

DOI: 10.3390/PR11061711

Abstract: Mushrooms have a long history of use as food and medicine. They are rich in various nutrients and bioactive compounds, particularly phenolic compounds. In this study, ten mushroom species were selected, and solvent extraction using 80% ethanol was used to extract phenolic compounds. Total phenolic content (TPC), total flavonoid content (TFC) and total condensed tannin content (TCT) were measured to evaluate phenolic content in different mushroom varieties. In the mushroom varieties tested, brown portobello mushroom had the highest TPC (396.78 ± 3.12 µg GAE/g), white cup mushroom exhibited the highest TFC (275.17 ± 9.40 μg CE/g), and shiitake mushroom presented the highest TCT (13.80 ± 0.21 µg QE/g). Antioxidant capacity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2,2′-azino-bis-3ethylbenzothiazoline-6-sulfonic acid (ABTS) and total antioxidant capacity (TAC) assays. The highest DPPH free radical scavenging ability was found in white cup mushroom (730.14 ± 55.06 µg AAE/g), while the greatest iron-reducing ability (FRAP) was recorded for shiitake mushroom (165.32 ± 10.21 μg AAE/g). Additionally, Swiss brown mushroom showed the highest ABTS antioxidant capacity (321.31 ± 5.7 μg AAE/g), and the maximum TAC value was found in shiitake mushroom (24.52 ± 1.2 μg AAE/g). These results highlight that most of the mushroom varieties studied showed high phenolic contents and demonstrated strong antioxidant activity, with shiitake mushrooms standing out due to their high TCT and FRAP values, and the highest TAC value among the varieties studied. In addition, LC-ESI-QTOF-MS/MS was used to characterize the mushroom s les, and tentatively identified a total of 22 phenolic compounds, including 11 flavonoids, 4 lignans, 3 phenolic acids, 2 stilbenes and 2 other phenolic compounds in all mushroom s les. The research results of this study showed that mushrooms are a good source of phenolic compounds with strong antioxidant potential. The results can provide a scientific basis for the development of mushroom extracts in functional food, health products, and other industries.

A test of the lipostat theory in a seasonal (ovine) model under natural conditions reveals a close relationship between adiposity and melanin concentrating hormone expression

Publisher: Elsevier BV

Date: 04-2009

DOI: 10.1016/J.DOMANIEND.2008.12.002

Abstract: A study was undertaken in Corriedale ewes to test the lipostat theory using data obtained from a model of seasonal change in food intake and body composition. The theory predicts adipose-derived factors signal to the brain and vice versa, to maintain homeostasis. It is held that leptin acts on cells in the brain to regulate food intake and energy expenditure, through "first order" neurons in the arcuate nucleus (ARC). These cells are thought to receive information that is relayed to "second order" neurons, to regulate food intake and other functions. In this study, groups (n=4-5) of ovariectomized ewes were maintained under natural conditions and s led at various points across the year. Food intake, body composition and indices of metabolic function were measured prior to collection of brains for in situ hybridization analysis. Expression of genes encoding for neuropeptide Y (NPY), pro-opiomelanocortin (POMC), orexin (ORX), melanin concentrating hormone (MCH) and leptin receptor (ObRb) was quantified. NPY gene expression was high when food intake was also high but, across the year, changes in NPY and POMC gene expression did not correspond predictably to plasma leptin levels or leptin receptor gene expression. Negative correlation was found between adiposity (omental and whole body fat) and gene expression of MCH and ORX, suggesting that changes in expression of genes for "second order" orexigenic peptides are closely linked to changes of metabolic state, even when similar relationships cannot be shown for expression of genes in "first order" neurons. These data provide support for the lipostat theory.

Session 2: Livestock Nutrition

Publisher: Wiley

Date: 11-2002

DOI: 10.1046/J.1440-6047.11.S5.2.X

Evaluation of solvent-extracted canola meal for growing pigs and lactating sows

Publisher: CSIRO Publishing

Date: 2001

DOI: 10.1071/AR01011

Abstract: The response of weaner pigs, grower/finisher pigs, and lactating sows to inclusion levels of solventextracted canola meal up to 250, 300, and 202 g/kg, respectively, was examined in 3 experiments. The average composition of the canola meal used in the experiments was 884 g DM/kg, 376 g CP/kg, 41 g fat/kg, 21.6 g lysine/kg, 12.4 MJ digestible energy (DE)/kg, and 4.5 mol glucosinolates/g. In all experiments, canola meal replaced other common protein sources in equal DE and equal lysine diets that were offered ad libitum to pigs. In Expt 1, 49 male pigs were weaned at 19.6 days of age and 6.27 kg liveweight and allocated to 7 diets containing 0–250 g/kg of canola meal for 42 days. Growth rate and voluntary feed intake were not significantly affected by level of canola meal up to 250 g/kg. However, there was a significant linear response in feed conversion ratio (FCR), indicating that as the level of canola meal increased, feed efficiency was improved. In Expt 2, pigs were randomly allocated at about 63 days of age and 24 kg liveweight to 16 group pens, each containing 12 pigs. Four pens were each allocated to one of 4 diets containing 0–300 g/kg canola meal up to slaughter at 140 days of age when pigs were approximately 97 kg liveweight. Canola meal level had no significant effect on growth performance, carcass quality, or weight of the thyroid gland. In Expt 3, 386 mixed parity sows were randomly allocated over a 70-day period to diets containing 0, 101, and 202 g/kg of canola meal that were offered during the lactation period of about 25 days. Average piglet growth between Day 3 and weaning was 244 g/day and was unaffected by inclusion level of canola meal in the sow diet. However, there was a significant positive linear response between sow feed intake and canola meal, which was particularly evident in the sows allocated during the initial period of the experiment when environmental temperatures were hotter. Average feed intake during lactation was 5.08, 5.50, and 5.67 for sows offered 0, 101, and 202 g/kg of canola meal, respectively. In conclusion, solvent-extracted canola meal can be included at levels up to 250, 300, and 202 g/kg in weaner pig, grower/finisher pig, and lactating sow diets, respectively, without adversely affecting the performance of these classes of pigs.

Current recommended levels of dietary lysine in finisher pig diets are sufficient to maximise the response to ractopamine over 28 days but are insufficient in the first 7 days

Publisher: CSIRO Publishing

Date: 2013

DOI: 10.1071/AN11348

Abstract: Dietary ractopamine increases lean tissue deposition and responses increase as dose is increased provided sufficient dietary lysine is supplied. In Australia, diets supplemented with ractopamine (RAC) are formulated with 0.56 g available lysine per MJ digestible energy. The present study was conducted to investigate the interactions between dietary RAC and lysine on growth and carcass characteristics in ad libitum fed (13.8 MJ/kg) boars and gilts. The study involved 108 in idually penned pigs at 17 weeks of age (64.1 ± 0.57 kg) in a 2 by 2 by 3 factorial design, with the respective factors being sex (gilt or boar), dietary lysine (low and high, i.e. 0.56 or 0.65 g available lysine/MJ digestible energy, respectively) and dietary RAC (0, 5 or 20 mg/kg) for 28 days. Over the 28-day study duration, both lysine diets containing dietary RAC were sufficient to elicit a response in average daily gain (ADG) (+5.8%, P = 0.026) and carcass weight (3%, P = 0.045), but not in feed efficiency (FE) (P = 0.555). However, over the period of the first 7 days, there were interactions between the effects of RAC and lysine for FE (P = 0.025) and ADG (P = 0.023), with both traits being responsive only to dietary RAC containing the high lysine, which increased FE (+9.1%, P = 0.002) and ADG (+7.2%, P = 0.068). Dietary RAC improved FE in the latter stages of the study, namely Days 15–21 (+5.7%, P = 0.031) and Days 22–28 (+4.9%, P = 0.040). The high RAC diet reduced carcass P2 backfat (–16.5%, P 0.001) and fat tissue deposition (–6.2%, P = 0.074) and high lysine tended to reduce fat tissue deposition (–13.3%, P = 0.072). A sex by lysine interaction (P = 0.043) was observed for lean tissue deposition at 28 days, such that only the high-lysine diet increased lean deposition in boars (+11%, P 0.05) but not in gilts. Dietary RAC tended to increase lean deposition (+14.0%, P = 0.067) in the first 14 days however, only the high RAC diet increased lean deposition (+9.6%, P 0.05) over 28 days. In conclusion, the current recommended supplementation levels of lysine for commercial gilts and boars fed RAC may limit the response to dietary RAC if the feeding regime is for short durations and boars will not maximise their lean tissue deposition rates.

The effect of immunization against GnRF on nutrient requirements of male pigs: a review

Publisher: Elsevier BV

Date: 2013

DOI: 10.1017/S1751731113001407

Impacts of Heat Stress on the Physiological and Production Responses of Lactating Dairy Cows Grazing Pastures over Hot Summer Months

Publisher: MDPI

Date: 19-01-2020

DOI: 10.3390/PROCEEDINGS2019036062

The influence of dietary magnesium supplement type, and supplementation dose and duration, on pork quality and the incidence of PSE pork

Publisher: CSIRO Publishing

Date: 2000

DOI: 10.1071/AR99090

Abstract: Ninety crossbred (Large White × Landrace) female pigs were used to compare the effects on ultimate pork quality of different magnesium (Mg) compounds [magnesium aspartate (MgAsp) and magnesium sulfate (MgSO4)], Mg dose (1.6 and 3.2 g elemental Mg), and Mg supplementation period (2 and 5 days) in pigs subjected to an acute stressor pre-slaughter. Pigs fed the Mg-supplemented diets had lower muscle lactic acid concentrations at 24 h post-slaughter than pigs fed the control diet. There were no significant differences in muscle glycogen concentrations in the Longissimus thoracis (LT) at 5 min, 40 min, or 24 h post-slaughter between the control and Mg-supplemented diets. Pigs fed the Mg-supplemented diets produced less pale colour pork (i.e. darker colour) and had reduced drip loss and reduced incidence of pale, soft, exudative (PSE) pork in the LT muscle compared with pigs fed the control diet. Within the Mg diets, pigs fed the MgAsp-supplemented diet produced less pale LT muscle colour than pigs fed the MgSO4-supplemented diet. There were no significant differences in LT pork quality parameters (colour and percent drip loss) regardless of whether the high or low Mg dose or the 2- or 5-day supplementation period was used. This experiment has shown that the use of dietary MgAsp supplementation at a dose of 1.6 g elemental Mg (20 g MgAsp) for 2 days prior to slaughter significantly improved pork quality and reduced the incidence of PSE pork.

LC-ESI-QTOF-MS/MS Profiling and Antioxidant Activity of Phenolics from Custard Apple Fruit and By-Products

Publisher: MDPI AG

Date: 09-05-2021

DOI: 10.3390/SEPARATIONS8050062

Abstract: Custard apple is an edible fruit grown in tropical and subtropical regions. Due to its abundant nutrient content and perceived health benefits, it is a popular food for consumption and is utilized as a medicinal aid. Although some published research had provided the phenolic compound of custard apple, the comprehensive phenolic profiling of Australian grown custard apple is limited. Hence, this research aimed to evaluate the phenolic content and antioxidant potential by various phenolic content and antioxidant assays, followed by characterization and quantification of the phenolic profile using LC-ESI-QTOF-MS/MS and HPLC-PDA. African Pride peel had the highest value in TPC (61.69 ± 1.48 mg GAE/g), TFC (0.42 ± 0.01 mg QE/g) and TTC (43.25 ± 6.70 mg CE/g), followed by Pink’s Mammoth peel (19.37 ± 1.48 mg GAE/g for TPC, 0.27 ± 0.03 mg QE/g for TFC and 10.25 ± 1.13 mg CE/g for TTC). African Pride peel also exhibited the highest antioxidant potential for TAC (43.41 ± 1.66 mg AAE/g), FRAP (3.60 ± 0.14 mg AAE/g) and ABTS (127.67 ± 4.60 mg AAE/g), whereas Pink’s Mammoth peel had the highest DPPH (16.09 ± 0.34 mg AAE/g), RPA (5.32 ± 0.14 mg AAE/g), •OH-RSA (1.23 ± 0.25 mg AAE/g) and FICA (3.17 ± 0.18 mg EDTA/g). LC-ESI-QTOF-MS/MS experiment successfully characterized 85 phenolic compounds in total, encompassing phenolic acids (20), flavonoids (42), stilbenes (4), lignans (6) and other polyphenols (13) in all three parts (pulp, peel and seeds) of custard apple. The phenolic compounds in different portions of custard apples were quantified by HPLC-PDA, and it was shown that African Pride peel had higher concentrations of the most abundant phenolics. This is the first study to provide the comprehensive phenolic profile of Australian grown custard apples, and the results highlight that each part of custard apple can be a rich source of phenolics for the utilization of custard apple fruit and waste in the food, animal feeding and nutraceutical industries.

The β3-adrenergic agonist (BRL35135A) acutely increases oxygen consumption and plasma intermediate metabolites in sheep

Publisher: CSIRO Publishing

Date: 2011

DOI: 10.1071/AN10019

Abstract: There is evidence that an atypical adrenoreceptor subtype is involved in mediating some of the physiological effects of catecholamines, particularly in some adipose tissue sites. Therefore, three experiments were conducted to determine the metabolic and energetic responses to oral administration of the purported β3-agonist BRL35135A in ruminant lambs. The post-prandial increase in O2 consumption (0.109 versus 0.139 L/min) and CO2 production (0.102 versus 0.127 L/min) at 30 min after feeding was greater (P 0.05) in the lambs receiving 5 mg of the BRL35135A. Treatment × time interactions over the period between –50 and 220 min indicate significant increases in plasma non-esterified fatty acids (P 0.001), glucose (P 0.001) and lactate (P = 0.024) in lambs consuming a single oral dose of 5 mg BRL35135A. In a subsequent experiment there were similar interactions over the period between –120 and 1440 min for non-esterified fatty acids (P 0.001), glucose (P 0.001) and lactate (P 0.001) in lambs consuming a lower oral dose of 1 mg BRL35135A. The effects of BRL35135A on plasma non-esterified fatty acids (P = 0.95), glucose (P = 0.84) and lactate (P = 0.68) were not modified by the β1- and β2-adrenergic antagonist alprenolol suggesting that the effects were mediated via β3-adrenergic receptor subtypes. In conclusion, these experiments indicate that BRL35135A is acutely active in sheep when given with feed, as indicated by increases in respiratory gas exchange and plasma metabolite concentrations.

Association of Thermotolerance with Milk Production, Feed Saver, Fertility and Fat Percentage Breeding Values in Holstein Friesian Dairy Cattle

Publisher: MDPI

Date: 19-01-2020

DOI: 10.3390/PROCEEDINGS2019036063

LC-MS/MS-QTOF Screening and Identification of Phenolic Compounds from Australian Grown Herbs and Their Antioxidant Potential

Publisher: MDPI AG

Date: 05-11-2021

DOI: 10.3390/ANTIOX10111770

Abstract: Culinary spices and herbs have been used to impart a characteristic flavour and aroma in food due to their appealing fragrance. Recently, bioactive compounds from herbs, especially phenolics, have gained much attention due to their potential health outcomes. The aim of this study was to characterize and quantify the phenolic compounds from 10 widely used Australian-grown herbs (oregano, rosemary, bay, basil, sage, fenugreek, dill, parsley, mint and thyme). For this purpose, liquid chromatography mass spectrometry (LC-MS) was used for the complete profiling of polyphenolic compounds and quantification of abundant phenolic compounds was completed with high-performance liquid chromatography—photodiode array detection (HPLC-PDA). Polyphenols from Australian-grown herbs were estimated through total phenolic content (TP), total flavonoids (TF) and total tannins (TT) along with their in-vitro antioxidant activities. Oregano and mint were estimated with the highest value of TP (140.59 ± 9.52 and 103.28 ± 8.08 mg GAE/g, milligram gallic acid equivalent/gram) while rosemary and mint had the highest TF (8.19 ± 0.74 and 7.05 ± 0.43 mg QE (quercetin equivalent)/g). In this study, eighty-four (84) phenolic compounds were screened and confirmed through LC-MS/MS by comparing their masses and fragmentation pattern with published libraries. The results of this study validate the use of these herbs as bioactives and their positive impact on human health.

The impact of antioxidant supplementation and heat stress on carcass characteristics, muscle nutritional profile and functionality of lamb meat

Publisher: MDPI AG

Date: 28-07-2020

DOI: 10.3390/ANI10081286

Abstract: The impact of antioxidant supplementation and short-term heat stress on lamb body weight gain, meat nutritional profile and functionality (storage stability of lipids and colour) of lamb meat was investigated. A total of 48 crossbred ((Merino × Border Leicester) × Dorset) lambs (42 ± 2 kg body weight, 7 mo age) were randomly allocated to three dietary treatments (n = 16) by liveweight (LW) that differed in dosage of vitamin E and selenium (Se) in the diet. Vitamin E and Se levels in the control (CON), moderate (MOD) and supranutritional (SUP) dietary treatments were 28, 130 and 228 mg/kg DM as α-tocopherol acetate and 0.16, 0.66 and 1.16 mg Se as SelPlex™/kg DM, respectively. After four weeks of feeding in in idual pens, including one week of adaptation, lambs were exposed to two heat treatments. Animals were moved to metabolism cages for one week and subjected to heat treatments: thermoneutral (TN 18–21 °C and 40–50% relative humidity) and heat stress (HS 28–40 °C and 30–40% relative humidity) conditions, respectively. Final LW and hot carcass weight were influenced by dietary treatments with higher final live weight (FLW) (p = 0.05 46.8 vs. 44.4 and 43.8 kg, respectively) and hot carcass weight (HCW) (p = 0.01 22.5 vs. 21.3 and 21.0 kg, respectively) recorded in lambs fed the SUP as opposed to the CON and MOD diets. Vitamin E concentration in the longissimus lumborum (LL) muscle tended to be higher in lambs fed MOD or SUP diets than the CON group. Lipid oxidation of aged meat at 72 h of simulated retail display was reduced by antioxidant supplementation. Short-term (one week) heat stress treatment significantly increased muscle linoleic acid and total omega-6 concentrations compared with the CON group. The results demonstrate that four-week antioxidant supplementation at the SUP level improved animal productivity by increasing LW and carcass weight and the functionality of meat exhibited by reduced lipid oxidation. An increase in muscle omega-6 fatty acid concentration from short-term heat stress may induce oxidative stress via proinflammatory action.

Consumer acceptability, eye fixation, and physiological responses: A study of novel and familiar chocolate packaging designs using eye-tracking devices

Publisher: MDPI AG

Date: 12-07-2019

DOI: 10.3390/FOODS8070253

Abstract: Eye fixations on packaging elements are not necessarily correlated to consumer attention or positive emotions towards those elements. This study aimed to assess links between the emotional responses of consumers and the eye fixations on areas of interest (AOI) of different chocolate packaging designs using eye trackers. Sixty participants were exposed to six novel and six familiar (commercial) chocolate packaging concepts on tablet PC screens. Analysis of variance (ANOVA) and multivariate analysis were performed on eye tracking, facial expressions, and self-reported responses. The results showed that there were significant positive correlations between liking and familiarity in commercially available concepts (r = 0.88), whereas, with novel concepts, there were no significant correlations. Overall, the total number of fixations on the familiar packaging was positively correlated (r = 0.78) with positive emotions elicited in people using the FaceReader™ (Happy), while they were not correlated with any emotion for the novel packaging. Fixations on a specific AOI were not linked to positive emotions, since, in some cases, they were related to negative emotions elicited in people or not even associated with any emotion. These findings can be used by package designers to better understand the link between the emotional responses of consumers and their eye fixation patterns for specific AOI.

Relations between plasma non-esterified fatty acid metabolism and body fat mobilization in primiparous lactating goats

Publisher: Cambridge University Press (CUP)

Date: 07-1989

DOI: 10.1079/BJN19890007

Abstract: During early lactation ruminants can mobilize considerable amounts of body fat to maintain milk production. The aim of the present study was to evaluate the efficacy of tritiated water (TOH) and non-esterified fatty acid (NEFA) kinetics as means of monitoring adipose tissue fat mobilization in lactating goats. Body fat, as estimated by a two-pool model of TOH kinetics, and NEFA entry rate were measured in four primiparous goats at days 11, 37 and 72 post partum. Estimated body fat decreased by an average of 64 g/d between days 11 and 37 of lactation, tending to increase between days 37 and 72. Plasma NEFA concentrations and NEFA entry rate decreased as lactation advanced, being significantly lower at day 72 than at day 11 of lactation. Both plasma concentrations of NEFA and NEFA entry rate were negatively correlated with calculated energy balance. Plasma NEFA concentrations and NEFA entry rate at days 11 and 37 of lactation were positively related to average body fat losses over the subsequent stage of lactation. These results demonstrate that NEFA kinetics reflect fat mobilization in primiparous lactating goats, particularly during negative energy balance.

Evaluation of common vetch (Vicia sativa cv. Morava) for growing pigs

Publisher: CSIRO Publishing

Date: 2005

DOI: 10.1071/EA04111

Abstract: The response to the inclusion of common vetch (Vicia sativa cv. Morava) at levels up to 225 g/kg diet was evaluated in growing pigs between 91 and 161 days of age. A total of 312 male pigs, selected at a liveweight of 37.6 ± 0.24 kg, were group housed in pens of 19–20 pigs, each initially in commercial grower facilities, which were then split within treatment into pens of 9–10 pigs in commercial finisher facilities. Pens were randomly allocated to 1 of 4 diets, 4 pens per diet. Vetch replaced peas in the wheat-based, protein-adequate diets at levels of 0, 75, 150 and 225 g/kg diet, with the digestible energy and lysine levels being similar for each diet. Pigs were offered the experimental grower diets for 4 weeks, followed by the corresponding experimental finisher diet for 6 weeks. Growth performance was not significantly affected by level of vetch up to 225 g/kg during the first 4 weeks of feeding with average growth rate, feed intake and feed conversion efficiency being 871.5 g/day, 1.90 kg/day, and 2.20 kg feed/kg gain, respectively. However, a significant negative linear response was observed for feed intake in pigs between 119 and 161 days of age, which resulted in voluntary feed intake decreasing from 2.62 to 2.44 kg/day when inclusion of Morava vetch increased from 0 to 225 g/kg. A significant curvilinear response was also observed in growth rate and feed conversion ratio during this period. These significant effects during the later growth phase resulted in significant decreases in voluntary feed intake and growth rate from 2.34 to 2.17 kg/day, and from 948 to 873 g/day, respectively, for the entire growth period as Morava vetch was increased from 0 to 225 g/kg diet. Thus, it is suggested that Morava vetch could be fed at rates up to 225 g/kg for pigs between 91 and 119 days of age, but should be reduced to less than 150 g/kg if feeding continues beyond 119 days of age.

The apparent ileal digestibility of amino acids in common vetch (Vicia sativa cv. Morava)

Publisher: CSIRO Publishing

Date: 2005

DOI: 10.1071/EA04112

Abstract: A total in of 12 entire male pigs were selected at about 32 kg liveweight and randomly allocated to 2 dietary treatments containing 0 and 400 g/kg vetch (Vicia sativa cv. Morava), respectively. Pigs were offered 1.3 kg of their respective diets for 15 days. The diets also contained 0.2 g/kg n-hextriacontane as an indigestible marker to allow the determination of amino acid digestibility at the terminal ileum. On day 15, pigs were anaesthetised, a ventral abdominal midline incision made and s les of faecal and ileal digesta collected. S les were used to measure the apparent ileal digestibility of the amino acids in the diets and the amino acid digestibility in vetch was calculated by difference. The apparent ileal digestibilities of lysine, threonine, methionine and arginine were 71.4, 53.5, 52.1 and 78.4%, respectively. The apparent ileal digestibility of the amino acids in vetch were relatively high and suggest common vetch (Vicia sativa cv. Morava) has the potential to be a viable alternative protein source for use in pig diets.

University Of Melbourne

Location: Australia

View Organisation

La Trobe University - Melbourne Campus

Location: Australia

View Organisation

University Of Leeds

Location: United Kingdom of Great Britain and Northern Ireland

View Organisation

Industrial Transformation Research Hubs - Grant ID: IH120100053

Start Date: 05-2014

End Date: 05-2018

Amount: $2,000,000.00

Funder: Australian Research Council

Linkage Projects - Grant ID: LP190101161

Start Date: 05-2021

End Date: 05-2025

Amount: $852,000.00

Funder: Australian Research Council