ORCID Profile
0000-0002-9109-051X
Current Organisations
Harvard University T H Chan School of Public Health
,
Harvard University
,
Home Tuitin Providers
,
Econexus Ventures Limited
,
Maiseville Groupe
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Publisher: Wiley
Date: 10-02-2020
DOI: 10.1002/JMV.25695
Publisher: Informa UK Limited
Date: 20-06-2021
Publisher: Elsevier BV
Date: 02-2022
Publisher: Wiley
Date: 13-06-2023
Abstract: Protein‐based nanomaterials have broad applications in the biomedical and bionanotechnological sectors owing to their outstanding properties such as high biocompatibility and biodegradability, structural stability, sophisticated functional versatility, and being environmentally benign. They have gained considerable attention in drug delivery, cancer therapeutics, vaccines, immunotherapies, biosensing, and biocatalysis. However, so far, in the battle against the increasing reports of antibiotic resistance and emerging drug‐resistant bacteria, unique nanostructures of this kind are lacking, hindering their potential next‐generation antibacterial agents. Here, the discovery of a class of supramolecular nanostructures with well‐defined shapes, geometries, or architectures (termed “protein nanospears”) based on engineered proteins, exhibiting exceptional broad‐spectrum antibacterial activities, is reported. The protein nanospears are engineered via spontaneous cleavage‐dependent or precisely tunable self‐assembly routes using mild metal salt‐ions (Mg 2+ , Ca 2+ , Na + ) as a molecular trigger. The nanospears’ dimensions collectively range from entire nano‐ to micrometer scale. The protein nanospears display exceptional thermal and chemical stability yet rapidly disassemble upon exposure to high concentrations of chaotropes ( mm sodium dodecyl sulfate (SDS)). Using a combination of biological assays and electron microscopy imaging, it is revealed that the nanospears spontaneously induce rapid and irreparable damage to bacterial morphology via a unique action mechanism provided by their nanostructure and enzymatic action, a feat inaccessible to traditional antibiotics. These protein‐based nanospears show promise as a potent tool to combat the growing threats of resistant bacteria, inspiring a new way to engineer other antibacterial protein nanomaterials with erse structural and dimensional architectures and functional properties.
Publisher: Wiley
Date: 18-02-2020
DOI: 10.1002/JMV.25706
Publisher: American Chemical Society (ACS)
Date: 04-10-2022
Abstract: Antibiotic resistance represents a serious global health concern and has stimulated the development of antimicrobial nanomaterials to combat resistant bacteria. Protein-based nanoparticles combining characteristics of both proteins and nanoparticles offer advantages including high biocompatibility, attractive biodegradability, enhanced bioavailability and functional versatility. They have played an increasing role as promising candidates for broad applications ranging from biocatalysts and drug delivery to vaccine development to cancer therapeutics. However, their application as antibacterial biomaterials to address challenging antibiotic-resistance problems has not been explicitly pursued. Herein, we describe engineering protein-only nanoparticles against resistant Gram-positive bacteria. A self-assembling peptide (P
Publisher: Informa UK Limited
Date: 15-12-2020
Publisher: Wiley
Date: 11-12-2020
Publisher: American Chemical Society (ACS)
Date: 11-08-2022
Abstract: Nitric oxide (NO)-releasing nanoparticles are effective nanomedicines with erse therapeutic advantages compared with small molecule-based NO donors. Here, we report a new class of furoxan-based NO-releasing nanoparticles using a simple, creative yet facile coassembly approach. This is the first time we demonstrated that the coassembled NO-releasing nanoparticles with poly(ethylene glycol)
Publisher: Elsevier BV
Date: 04-2021
Publisher: Wiley
Date: 03-03-2022
DOI: 10.1002/WNAN.1785
Abstract: The emergence of SARS‐COV‐2, the causative agent of new coronavirus disease (COVID‐19) has become a pandemic threat. Early and precise detection of the virus is vital for effective diagnosis and treatment. Various testing kits and assays, including nucleic acid detection methods, antigen tests, serological tests, and enzyme‐linked immunosorbent assay (ELISA), have been implemented or are being explored to detect the virus and/or characterize cellular and antibody responses to the infection. However, these approaches have inherent drawbacks such as nonspecificity, high cost, are characterized by long turnaround times for test results, and can be labor‐intensive. Also, the circulating SARS‐COV‐2 variant of concerns, reduced antibody sensitivity and/or neutralization, and possible antibody‐dependent enhancement (ADE) have warranted the search for alternative potent therapeutics. Aptamers, which are single‐stranded oligonucleotides, generated artificially by SELEX (Evolution of Ligands by Exponential Enrichment) may offer the capacity to generate high‐affinity neutralizers and/or bioprobes for monitoring relevant SARS‐COV‐2 and COVID‐19 biomarkers. This article reviews and discusses the prospects of implementing aptamers for rapid point‐of‐care detection and treatment of SARS‐COV‐2. We highlight other SARS‐COV‐2 targets (N protein, spike protein stem‐helix), SELEX augmented with competition assays and in silico technologies for rapid discovery and isolation of theranostic aptamers against COVID‐19 and future pandemics. It further provides an overview on site‐specific bioconjugation approaches, customizable molecular scaffolding strategies, and nanotechnology platforms to engineer these aptamers into ultrapotent blockers, multivalent therapeutics, and vaccines to boost both humoral and cellular immunity against the virus. This article is categorized under: Therapeutic Approaches and Drug Discovery Emerging Technologies Diagnostic Tools Biosensing Therapeutic Approaches and Drug Discovery Nanomedicine for Infectious Disease Therapeutic Approaches and Drug Discovery Nanomedicine for Respiratory Disease
Location: United States of America
No related grants have been discovered for Christian K.O Dzuvor.