ORCID Profile
0000-0002-2331-127X
Current Organisation
University of Nottingham
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Publisher: American Society for Clinical Investigation
Date: 03-05-2010
DOI: 10.1172/JCI41560
Publisher: Elsevier BV
Date: 09-2005
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0BM00709A
Abstract: Silicone catheters impregnated with antibiotics and coated with an anti-attachment polyacrylate produce a device with dual anti-biofilm and anti-bacterial properties.
Publisher: American Vacuum Society
Date: 05-2023
DOI: 10.1116/6.0002604
Abstract: Secondary ion mass spectrometry (SIMS) offers advantages over both liquid extraction mass spectrometry and matrix assisted laser desorption mass spectrometry in that it provides the direct in situ analysis of molecules and has the potential to preserve the 3D location of an analyte in a s le. Polysaccharides are recognized as challenging analytes in the mass spectrometry of liquids and are also difficult to identify and assign using SIMS. Psl is an exopolysaccharide produced by Pseudomonas aeruginosa, which plays a key role in biofilm formation and maturation. In this Letter, we describe the use of the OrbiTrap analyzer with SIMS (3D OrbiSIMS) for the label-free mass spectrometry of Psl, taking advantage of its high mass resolving power for accurate secondary ion assignment. We study a P. aeruginosa biofilm and compare it with purified Psl to enable the assignment of secondary ions specific to the Psl structure. This resulted in the identification of 17 peaks that could confidently be ascribed to Psl fragments within the biofilm matrix. The complementary approach of the following neutral loss sequences is also shown to identify multiple oligosaccharide fragments without the requirement of a biological reference s le.
Publisher: Wiley
Date: 25-04-2006
Abstract: Mannose receptor (MR) is the best characterised member of a family of four endocytic molecules that share a common domain structure a cysteine-rich (CR) domain, a fibronectin-type II (FNII) domain and tandemly arranged C-type lectin-like domains (CTLD, eight in the case of MR). Two distinct lectin activities have been described for MR. The CR domain recognises sulphated carbohydrates while the CTLD mediate binding to mannose, fucose or N-acetylglucosamine. FNII domains are known to be important for collagen binding and this has been studied in the context of two members of the MR family, Endo180 and the phospholipase A2 receptor. Here, we have investigated whether the broad and effective lectin activity mediated by the CR domain and CTLD of MR is favoured to the detriment of FNII-mediated interaction(s). We show that MR is able to bind and internalise collagen in a carbohydrate-independent manner and that MR deficient macrophages have a marked defect in collagen IV and gelatin internalisation. These data have major implications at the molecular level as there are now three distinct ligand-binding sites described for MR. Furthermore our findings extend the range of endogenous ligands recognised by MR, a molecule firmly placed at the interface between homeostasis and immunity.
Publisher: Public Library of Science (PLoS)
Date: 19-05-2010
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Luisa Martinez-Pomares.