ORCID Profile
0000-0002-6538-8566
Current Organisations
Charles Sturt University
,
NSW Department of Primary Industries
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Publisher: Elsevier BV
Date: 08-2014
DOI: 10.1016/J.JCYT.2014.01.010
Abstract: Numerous protocols for the isolation of bovine aortic endothelial cells have been described in the previous literature. However, these protocols prevent researchers from obtaining the pure population of endothelial cells. Thus, this study aimed to develop a new and economical method for the isolation of pure endothelial cells by introducing a new strategy to the enzymatic digestion method proposed by previous researchers. With the use of this method, the lumen of a bovine aorta was filled with wash medium and the outer surface of the s le was washed with alcohol for 30 seconds. Under a laminar flow hood, the inner surface of the s le was covered with filter paper. Collagenase type II was dripped onto the filter paper as a digestion enzyme. The digestion fluid was seeded in T25 flasks and fed with complete medium every 3 days. The isolated cells were characterized by markers such as CD31, von Willebrand factor, 1,1'-dioctadecyl-1,3,3,3',3'-tetramethylindocarbocyanine perchlorate acetylated low-density lipoprotein and angiogenesis behavior. The purity of endothelial cells was detected by flow cytometry to be of nearly 90% purity these results were confirmed by immunostaining. Moreover, endothelial cells formed blood vessel-like tubes in a three-dimensional environment, which is specific dynamic behavior for endothelial cells. The new strategy presented in the current report enables isolation of a highly pure population of endothelial cells that can survive long-term culture without inducing an overgrowth of fibroblast cells.
Publisher: Wiley
Date: 25-04-2014
DOI: 10.1002/JBM.A.35186
Abstract: Extracellular environments can regulate cell behavior because cells can actively sense their mechanical environments. This study evaluated the adhesion, proliferation and morphology of endothelial cells on polydimethylsiloxane (PDMS)/alumina (Al2 O3 ) composites and pure PDMS. The substrates were prepared from pure PDMS and its composites with 2.5, 5, 7.5, and 10 wt % Al2 O3 at a curing temperature of 50°C for 4 h. The substrates were then characterized by mechanical, structural, and morphological analyses. The cell adhesion, proliferation, and morphology of cultured bovine aortic endothelial (BAEC) cells on substrate materials were evaluated by using resazurin assay and 1,1'-dioctadecyl-1,3,3,3',3'-tetramethylindocarbocyanine perchlorate-acetylated LDL (Dil-Ac-LDL) cell staining, respectively. The composites (PDMS/2.5, 5, 7.5, and 10 wt % Al2 O3 ) exhibited higher stiffness than the pure PDMS substrate. The results also revealed that stiffer substrates promoted endothelial cell adhesion and proliferation and also induced spread morphology in the endothelial cells compared with lesser stiff substrates. Statistical analysis showed that the effect of time on cell proliferation depended on stiffness. Therefore, this study concludes that the addition of different Al2 O3 percentages to PDMS elevated substrate stiffness which in turn increased endothelial cell adhesion and proliferation significantly and induced spindle shape morphology in endothelial cells.
Publisher: Wiley
Date: 22-09-2015
DOI: 10.1002/JBM.A.35561
Abstract: Extracellular matrices have drawn attention in tissue engineering as potential biomaterials for scaffold fabrication because of their bioactive components. Noninvasive techniques of scaffold fabrication and cross-linking treatments are believed to maintain the integrity of bioactive molecules while providing proper architectural and mechanical properties. Cartilage matrix derived scaffolds are designed to support the maintenance of chondrocytes and provide proper signals for differentiation of chondroinducible cells. Chondroinductive potential of bovine articular cartilage matrix derived porous scaffolds on human dermal fibroblasts and the effect of scaffold shrinkage on chondrogenesis were investigated. An increase in sulfated glycosaminoglycans production along with upregulation of chondrogenic genes confirmed that physically treated cartilage matrix derived scaffolds have chondrogenic potential on human dermal fibroblasts.
Publisher: Wiley
Date: 28-07-2023
DOI: 10.1111/AVJ.13274
Abstract: Our study aimed to investigate responses to supplementation of calcium (Ca), magnesium (Mg) and sodium (Na) to lactating ewes and lambs grazing barley forage from lamb marking to weaning. A 10‐ha paddock sown to barley was sub ided into eight plots as four replicates of two treatments. Merino ewes (n = 104) with lambs at foot were stratified to the eight plots (13 ewes and 21 lambs lot) based on number of lambs (twin or single) and ewes' weight. Supplemented groups had access to mineral supplements (30 g/ewe/day) supplying 12 g/ewe/day ground limestone, 12 g/ewe/day Causmag® and 6 g/ewe/day coarse salt in a ratio of 2: 2: 1 by weight (as fed) from day 0 (a day before lamb marking) after s le collection. Control groups were not supplemented with minerals. Blood, milk and urine s les from ewes and blood from lambs were collected at different time points, namely, a day prior to lamb marking (day 0), 14 days after the commencement of study (day 14), and 28 days after the commencement of study (day 28). Weight of the lambs was also recorded at each time point. We found that the concentration of the forage minerals (Ca, Mg and potassium (K)) was lower on day 28 than on day 0 (P 0.025). Liveweight gain was greater in the first 14 days compared with the second weight gain period (P 0.001). The interaction of time and treatment was significant for liveweight (P 0.001). Due to the improvement in weight gain of supplemented lambs, we recommend that mineral supplementation during late lactation is beneficial considering the low cost of minerals, even though the mineral content of the forage was not deficient.
Publisher: Informa UK Limited
Date: 12-2014
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4AY00666F
Abstract: Archimedes revisited: computer assisted micro-volumetric modification of the liquid displacement method for porosity measurement of highly porous light materials.
Publisher: Springer Science and Business Media LLC
Date: 07-05-2015
DOI: 10.1038/SREP09806
Abstract: Scaffold design from xenogeneic bone has the potential for tissue engineering (TE). However, major difficulties impede this potential, such as the wide range of properties in natural bone. In this study, sintered cortical bones from different parts of a bovine-femur impregnated with biodegradable poly(ethylene glycol) (PEG) binder by liquid phase adsorption were investigated. Flexural mechanical properties of the PEG-treated scaffolds showed that the scaffold is stiffer and stronger at a sintering condition of 1000°C compared with 900°C. In vitro cytotoxicity of the scaffolds evaluated by Alamar Blue assay and microscopic tests on human fibroblast cells is better at 1000°C compared with that at 900°C. Furthermore, in vitro biocompatibility and flexural property of scaffolds derived from different parts of a femur depend on morphology and heat-treatment condition. Therefore, the fabricated scaffolds from the distal and proximal parts at 1000°C are potential candidates for hard and soft TE applications, respectively.
Publisher: Public Library of Science (PLoS)
Date: 08-02-2013
Publisher: CSIRO Publishing
Date: 21-04-2021
DOI: 10.1071/AN19139
Abstract: Context Dual-purpose crops (for grazing and grain) are an important part of the feedbase in mixed farming systems in the medium-rainfall zone of southern Australia. On these farms, non-wool sheep breeds such as the Dorper may provide an opportunity to increase lamb production while reducing labour costs compared with traditional sheep breeds. Aims This study was designed to compare lamb production systems based on White Dorper and Merino ewes joined to a terminal sire, while exploiting a feedbase that included dual-purpose crops. Methods Two experiments were conducted at Wagga Wagga, New South Wales, during 2013 (Expt 1) and 2014 (Expt 2). In February in both experiments, White Dorper ewes were joined to either White Dorper or White Suffolk rams (lambs designated DD and WSD, respectively), and Merino ewes were joined to White Suffolk rams (lambs designated WSM). In Expt 1 a dual-purpose wheat (Triticum aestivum L.) crop was established and the paddock sub ided into nine plots (0.93 ha each). Pregnant ewes were allocated to plots on the basis of genotype (DD, WSD or WSM grazing separate plots) after blocking for number of fetuses identified at mid-pregnancy scanning, with three replicates based on genotype. Lambing commenced on 28 June, and ewes grazed the crop from 27 June to 14 August and then continued to graze in the same groups on a lucerne (Medicago sativa L.)–clover (Trifolium spp.) pasture sub ided into nine plots (2.1 ha), maintaining the same replicates until weaning on 2 October. In Expt 2, the cropping paddock was sub ided into six plots (1.86 ha each) sown to either dual-purpose wheat or canola (Brassica napus L.), with three replicates. Lambing commenced on 13 July. Merino and White Dorper ewes grazed the crops concurrently from 19 June to 12 August, and then lucerne–clover pasture until weaning on 29 September. In both experiments a mineral supplement was fed to ewes grazing wheat during the crop-grazing period. Key results Feed on offer was low (0.33 t/ha) in dual-purpose wheat crops at the start of grazing in Expt 1 and did not differ among genotypes at the cessation of grazing crops. Feed on offer was higher in wheat than canola at the commencement of grazing in Expt 2 (2.6 vs 2.3 t/ha P = 0.009), and a higher stocking rate was maintained on wheat than canola from the start of lambing. White Dorper ewes maintained a higher body condition score than Merino ewes throughout both experiments. In Expt 2, some ewes had serum magnesium and calcium levels below the normal range, and a high number of ewes (20%) required assistance at lambing. Number of lambs weaned per ewe scanned pregnant was similar among genotypes in both experiments. WSD lambs were heaviest (P 0.05) at weaning and DD lambs had greatest (P 0.05) fat depth over the eye muscle at weaning. Conclusions and implications When grazed on a feedbase typical of mixed farms in the medium-rainfall zone of southern NSW, White Dorper ewes were in higher body condition than Merino ewes however, reproductive rates were similar. Joining White Dorper ewes to a terminal sire may increase weaning weight of lambs compared with joining to a White Dorper ram, or a Merino maternal system.
Publisher: Elsevier BV
Date: 11-2018
Publisher: Elsevier BV
Date: 06-2020
Publisher: No publisher found
Date: 2014
Location: United States of America
No related grants have been discovered for Forough Ataollahi.