ORCID Profile
0000-0002-5618-6321
Current Organisation
GNS Science
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Microbiology Society
Date: 04-2014
Abstract: A strictly aerobic, thermophilic, moderately acidophilic, non-spore-forming bacterium, strain P373 T , was isolated from geothermally heated soil at Waikite, New Zealand. Cells were filamentous rods, 0.2–0.4 µm in diameter and grew in chains up to 80 µm in length. On the basis of 16S rRNA gene sequence similarity, strain P373 T was shown to belong to the family Chitinophagaceae (class Sphingobacteriia ) of the phylum Bacteroidetes , with the most closely related cultivated strain, Chitinophaga pinensis UQM 2034 T , having 87.6 % sequence similarity. Cells stained Gram-negative, and were catalase- and oxidase-positive. The major fatty acids were i-15 : 0 (10.8 %), i-17 : 0 (24.5 %) and i-17 : 0 3-OH (35.2 %). Primary lipids were phosphatidylethanolamine, two unidentified aminolipids and three other unidentified polar lipids. The presence of sulfonolipids ( N -acyl-capnines) was observed in the total lipid extract by mass spectrometry. The G+C content of the genomic DNA was 47.3 mol% and the primary respiratory quinone was MK-7. Strain P373 T grew at 35–63 °C with an optimum temperature of 60 °C, and at pH 5.5–8.7 with an optimum growth pH of 7.3–7.4. NaCl tolerance was up to 5 % (w/v) with an optimum of 0.1–0.25 % (w/v). Cell colonies were non-translucent and pigmented vivid yellow–orange. Cells displayed an oxidative chemoheterotrophic metabolism. The distinct phylogenetic position and the phenotypic characteristics separate strain P373 T from all other members of the phylum Bacteroidetes and indicate that it represents a novel species in a new genus, for which the name Thermoflavifilum aggregans gen. nov., sp. nov. is proposed. The type strain of the type species is P373 T ( = ICMP 20041 T = DSM 27268 T ).
Publisher: Microbiology Society
Date: 12-2015
Abstract: An aerobic, thermophilic and cellulolytic bacterium, designated strain WKT50.2 T , was isolated from geothermal soil at Waikite, New Zealand. Strain WKT50.2 T grew at 53–76 °C and at pH 5.9–8.2. The DNA G+C content was 58.4 mol%. The major fatty acids were 12-methyl C 18 : 0 and C 18 : 0 . Polar lipids were all linked to long-chain 1,2-diols, and comprised 2-acylalkyldiol-1- O -phosphoinositol (diolPI), 2-acylalkyldiol-1- O -phosphoacylmannoside (diolP-acylMan), 2-acylalkyldiol-1- O -phosphoinositol acylmannoside (diolPI-acylMan) and 2-acylalkyldiol-1- O -phosphoinositol mannoside (diolPI-Man). Strain WKT50.2 T utilized a range of cellulosic substrates, alcohols and organic acids for growth, but was unable to utilize monosaccharides. Robust growth of WKT50.2 T was observed on protein derivatives. WKT50.2 T was sensitive to icillin, chlor henicol, kanamycin, neomycin, polymyxin B, streptomycin and vancomycin. Metronidazole, lasalocid A and trimethoprim stimulated growth. Phylogenetic analysis of 16S rRNA gene sequences showed that WKT50.2 T belonged to the class Thermomicrobia within the phylum Chloroflexi , and was most closely related to Thermorudis peleae KI4 T (99.6% similarity). DNA–DNA hybridization between WKT50.2 T and Thermorudis peleae DSM 27169 T was 18.0%. Physiological and biochemical tests confirmed the phenotypic and genotypic differentiation of strain WKT50.2 T from Thermorudis peleae KI4 T and other members of the Thermomicrobia . On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain WKT50.2 T represents a novel species, for which the name Thermorudis pharmacophila sp. nov. is proposed, with the type strain WKT50.2 T ( = DSM 26011 T = ICMP 20042 T ). Emended descriptions of Thermomicrobium roseum , Thermomicrobium carboxidum , Thermorudis peleae and Sphaerobacter thermophilus are also proposed, and include the description of a novel respiratory quinone, MK-8 2,3-epoxide (23%), in Thermomicrobium roseum .
Publisher: Springer Science and Business Media LLC
Date: 04-08-2017
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Karen Houghton.