ORCID Profile
0000-0002-2853-4421
Current Organisation
University Of Strathclyde
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Publisher: Elsevier BV
Date: 08-2014
DOI: 10.1016/J.JCYT.2014.01.010
Abstract: Numerous protocols for the isolation of bovine aortic endothelial cells have been described in the previous literature. However, these protocols prevent researchers from obtaining the pure population of endothelial cells. Thus, this study aimed to develop a new and economical method for the isolation of pure endothelial cells by introducing a new strategy to the enzymatic digestion method proposed by previous researchers. With the use of this method, the lumen of a bovine aorta was filled with wash medium and the outer surface of the s le was washed with alcohol for 30 seconds. Under a laminar flow hood, the inner surface of the s le was covered with filter paper. Collagenase type II was dripped onto the filter paper as a digestion enzyme. The digestion fluid was seeded in T25 flasks and fed with complete medium every 3 days. The isolated cells were characterized by markers such as CD31, von Willebrand factor, 1,1'-dioctadecyl-1,3,3,3',3'-tetramethylindocarbocyanine perchlorate acetylated low-density lipoprotein and angiogenesis behavior. The purity of endothelial cells was detected by flow cytometry to be of nearly 90% purity these results were confirmed by immunostaining. Moreover, endothelial cells formed blood vessel-like tubes in a three-dimensional environment, which is specific dynamic behavior for endothelial cells. The new strategy presented in the current report enables isolation of a highly pure population of endothelial cells that can survive long-term culture without inducing an overgrowth of fibroblast cells.
Publisher: Wiley
Date: 25-04-2014
DOI: 10.1002/JBM.A.35186
Abstract: Extracellular environments can regulate cell behavior because cells can actively sense their mechanical environments. This study evaluated the adhesion, proliferation and morphology of endothelial cells on polydimethylsiloxane (PDMS)/alumina (Al2 O3 ) composites and pure PDMS. The substrates were prepared from pure PDMS and its composites with 2.5, 5, 7.5, and 10 wt % Al2 O3 at a curing temperature of 50°C for 4 h. The substrates were then characterized by mechanical, structural, and morphological analyses. The cell adhesion, proliferation, and morphology of cultured bovine aortic endothelial (BAEC) cells on substrate materials were evaluated by using resazurin assay and 1,1'-dioctadecyl-1,3,3,3',3'-tetramethylindocarbocyanine perchlorate-acetylated LDL (Dil-Ac-LDL) cell staining, respectively. The composites (PDMS/2.5, 5, 7.5, and 10 wt % Al2 O3 ) exhibited higher stiffness than the pure PDMS substrate. The results also revealed that stiffer substrates promoted endothelial cell adhesion and proliferation and also induced spread morphology in the endothelial cells compared with lesser stiff substrates. Statistical analysis showed that the effect of time on cell proliferation depended on stiffness. Therefore, this study concludes that the addition of different Al2 O3 percentages to PDMS elevated substrate stiffness which in turn increased endothelial cell adhesion and proliferation significantly and induced spindle shape morphology in endothelial cells.
Publisher: Elsevier BV
Date: 05-2014
Publisher: Walter de Gruyter GmbH
Date: 2017
Abstract: While considering the importance of the interface between utees and prosthesis sockets, we study an anthropomorphic prosthesis socket whose size can be dynamically changed according to the requirements of the residual limb. First, we introduce the structure and function of the anthropomorphic prosthesis socket. Second, we study the dynamic model of the prosthesis system and analyze the dynamic characteristics of the prosthesis socket system, the inputs of an oscillometric pump, and the control mechanism of force-sensitive resistor (FSR) pressure signals. Experiments on 10 healthy subjects using the designed system yield an average detection result between 102 and 112 kPa for the FSR pressure sensor and 39 and 41 kPa for the oscillometric pump. Results show the function of the FSR pressure signal in maintaining the contact pressure between the sockets and the residual limb. The potential development of an auto-adjusted socket that uses an oscillometric pump system will provide prosthetic sockets with controllable contact pressure at the residual limb. Moreover, this development is an attractive research area for researchers involved in rehabilitation engineering, prosthetics, and orthotics.
Publisher: SAGE Publications
Date: 27-05-2020
Abstract: The conservative techniques of treating knee osteoarthritis (kOA) include wearing orthoses such as knee braces and laterally wedged insoles and applying gait modification techniques such as toe-in gait and toe-out gait. This study aimed at assessing the immediate effects of these techniques in improving physical function of healthy and kOA participants. Five Osteoarthritis Research Society International (OARSI) recommended performance-based tests were randomly applied to measure physical function: (1) 30-second chair stand test (30CST), (2) 40-m (4 × 10) fast-paced walk test (40FPW), (3) stair climb test (SCT), (4) timed up and go test (TUGT) and (5) 6-minute walk test (6MWT) during a single-visit on 20 healthy and 20 kOA patients (age: 59.5 ± 7.33 and 61.5 ± 8.63 years, BMI: 69.95 ± 9.86 and 70.45 ± 8.80 kg/m 2 ). The interventions included natural gait, toe-out gait, toe-in gait, laterally wedged insoles and knee brace. Analysis was performed through repeated-measures ANOVA and independent s le t-test. 30CST and TUGT showed no significant differences for the five test conditions ( p 0.05). Toe-out showed profound effects via pairwise comparison in impairing the physical function while knee brace improved it during 40FPW, SCT and 6MWT. In general, all the tested conservative techniques except laterally wedged insoles had immediate effects on physical performance measures in both healthy and medial knee osteoarthritis participants. The valgus knee brace improved the parameters the most, while toe-out gait impaired them the most. Future studies can develop strategies for improving gait retraining methods on the basis of issues identified by this study.
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C5RA17326D
Abstract: This study describes how various loadings of two ions with different size and charge, such as silver and tantalum, can affect the mechanical and biological properties of calcium silicate (CS).
Publisher: Sergii Iermakov
Date: 30-04-2023
DOI: 10.15561/26649837.2023.0205
Abstract: Background and Study Aim. Established studies show that athletes with longer fingers and broader hand surfaces have more muscular grips. Therefore, some research studies have examined various contributing factors and anthropometric characteristics. Thus, the aim of this study was to investigate the effect of hand dimensions and selected anthropometric characteristics on handgrip strength in recreational tenpin bowlers. Material and Methods. This cross-sectional study recruited 32 (12 females, 20 males) healthy Malaysian recreational tenpin bowlers from Kuala Lumpur. Their anthropometric characteristics including height, weight, body mass index (BMI), the dimensions of the right hand, and age were measured accordingly. Handgrip strength was assessed using a Takei 5401 Grip D (Digital Grip Dynamometer) with 3 trials for both hands. A Pearson correlation coefficient and multiple regression analysis were used to study the relationship between the parameters. Results. The body height and the minimum breadth of the right hand had a significant impact on handgrip strength among recreational tenpin bowlers. There was a significant difference between males and females in left and right handgrip strength (p .05). Males showed a greater handgrip strength compared to females in both hands’ strength. Body height (p = 0.00) and the minimum breadth of the hand (p = 0.03) were found to be significantly correlated with the handgrip strength thus indicating the two variables as strong predictors of handgrip strength. Conclusions. This study confirms that there is a relationship between anthropometric characteristics and handgrip strength in Malaysian recreational tenpin bowlers. Hence, it will be a great note for new bowlers to advance their bowling performance.
Publisher: American Chemical Society (ACS)
Date: 09-07-2014
DOI: 10.1021/AM500845X
Abstract: Calcium silicate (CaSiO3, CS) ceramics are promising bioactive materials for bone tissue engineering, particularly for bone repair. However, the low toughness of CS limits its application in load-bearing conditions. Recent findings indicating the promising biocompatibility of graphene imply that graphene can be used as an additive to improve the mechanical properties of composites. Here, we report a simple method for the synthesis of calcium silicate/reduced graphene oxide (CS/rGO) composites using a hydrothermal approach followed by hot isostatic pressing (HIP). Adding rGO to pure CS increased the hardness of the material by ∼40%, the elastic modulus by ∼52%, and the fracture toughness by ∼123%. Different toughening mechanisms were observed including crack bridging, crack branching, crack deflection, and rGO pull-out, thus increasing the resistance to crack propagation and leading to a considerable improvement in the fracture toughness of the composites. The formation of bone-like apatite on a range of CS/rGO composites with rGO weight percentages ranging from 0 to 1.5 has been investigated in simulated body fluid (SBF). The presence of a bone-like apatite layer on the composite surface after soaking in SBF was demonstrated by X-ray diffraction (XRD) and field emission scanning electron microscopy (FESEM). The biocompatibility of the CS/rGO composites was characterized using methyl thiazole tetrazolium (MTT) assays in vitro. The cell adhesion results showed that human osteoblast cells (hFOB) can adhere to and develop on the CS/rGO composites. In addition, the proliferation rate and alkaline phosphatase (ALP) activity of cells on the CS/rGO composites were improved compared with the pure CS ceramics. These results suggest that calcium silicate/reduced graphene oxide composites are promising materials for biomedical applications.
Publisher: Informa UK Limited
Date: 02-2014
Publisher: MDPI AG
Date: 10-02-2014
DOI: 10.3390/MA7020980
Publisher: IEEE
Date: 08-2012
Publisher: IEEE
Date: 08-2012
Publisher: Public Library of Science (PLoS)
Date: 08-02-2013
Publisher: Elsevier BV
Date: 02-2014
DOI: 10.1016/J.JMBBM.2013.10.024
Abstract: The focus of this study is to investigate the effect of Al2O3 on α-calcium silicate (α-CaSiO3) ceramic. α-CaSiO3 was synthesized from CaO and SiO2 using mechanochemical method followed by calcinations at 1000°C. α-CaSiO3 and alumina were grinded using ball mill to create mixtures, containing 0-50w% of Al2O3 loadings. The powders were uniaxially pressed and followed by cold isostatic pressing (CIP) in order to achieve greater uniformity of compaction and to increase the shape capability. Afterward, the compaction was sintered in a resistive element furnace at both 1150°C and 1250°C with a 5h holding time. It was found that alumina reacted with α-CaSiO3 and formed alumina-rich calcium aluminates after sintering. An addition of 15wt% of Al2O3 powder at 1250°C were found to improve the hardness and fracture toughness of the calcium silicate. It was also observed that the average grain sizes of α-CaSiO3 /Al2O3 composite were maintained 500-700nm after sintering process.
Publisher: Informa UK Limited
Date: 20-06-2015
Publisher: Wiley
Date: 14-08-2013
DOI: 10.1111/IJAC.12151
Publisher: Index Copernicus
Date: 30-01-2012
Publisher: Springer Science and Business Media LLC
Date: 07-05-2015
DOI: 10.1038/SREP09806
Abstract: Scaffold design from xenogeneic bone has the potential for tissue engineering (TE). However, major difficulties impede this potential, such as the wide range of properties in natural bone. In this study, sintered cortical bones from different parts of a bovine-femur impregnated with biodegradable poly(ethylene glycol) (PEG) binder by liquid phase adsorption were investigated. Flexural mechanical properties of the PEG-treated scaffolds showed that the scaffold is stiffer and stronger at a sintering condition of 1000°C compared with 900°C. In vitro cytotoxicity of the scaffolds evaluated by Alamar Blue assay and microscopic tests on human fibroblast cells is better at 1000°C compared with that at 900°C. Furthermore, in vitro biocompatibility and flexural property of scaffolds derived from different parts of a femur depend on morphology and heat-treatment condition. Therefore, the fabricated scaffolds from the distal and proximal parts at 1000°C are potential candidates for hard and soft TE applications, respectively.
Publisher: Public Library of Science (PLoS)
Date: 25-06-2013
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6RA21210G
Abstract: A model of a controlled drug release mechanism of a dielectric core–shell composite carrier.
Publisher: Public Library of Science (PLoS)
Date: 17-09-2014
Publisher: Springer Science and Business Media LLC
Date: 16-05-2013
Abstract: Despite the rapid advancement of left ventricular assist devices (LVADs), adverse events leading to deaths have been frequently reported in patients implanted with LVADs, including bleeding, infection, thromboembolism, neurological dysfunction and hemolysis. Cannulation forms an important component with regards to thrombus formation in assisted patients by varying the intraventricular flow distribution in the left ventricle (LV). To investigate the correlation between LVAD cannula placement and potential for thrombus formation, detailed analysis of the intraventricular flow field was carried out in the present study using a two way fluid structure interaction (FSI), axisymmetric model of a passive LV incorporating an inflow cannula. Three different cannula placements were simulated, with device insertion near the LV apex, penetrating one-fourth and mid-way into the LV long axis. The risk of thrombus formation is assessed by analyzing the intraventricular vorticity distribution and its associated vortex intensity, amount of stagnation flow in the ventricle as well as the level of wall shear stress. Our results show that the one-fourth placement of the cannula into the LV achieves the best performance in reducing the risk of thrombus formation. Compared to cannula placement near the apex, higher vortex intensity is achieved at the one-fourth placement, thus increasing wash out of platelets at the ventricular wall. One-fourth LV penetration produced negligible stagnation flow region near the apical wall region, helping to reduce platelet deposition on the surface of the cannula and the ventricular wall.
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for NOOR AZUAN ABU OSMAN.