ORCID Profile
0000-0002-9960-7579
Current Organisations
Centro Hospitalar Lisboa Norte EPE
,
Faculty of Medicine
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Publisher: Elsevier BV
Date: 2017
Publisher: Elsevier BV
Date: 07-2018
Publisher: BMJ
Date: 27-06-2018
Publisher: Wiley
Date: 09-2017
Publisher: Wiley
Date: 23-09-2015
Publisher: Wiley
Date: 21-11-2018
DOI: 10.1111/AOS.13622
Abstract: Previous studies report that the response of retinal vessels to a decrease in oxygen (hypoxia) is vasodilation, thus increasing blood flow. We aimed to characterize the changes in retinal microvasculature induced by a mild hypoxia stress test in a healthy population, using ocular coherence tomography angiography (OCT-A) technology. Interventional repeated-measures study. The standardized hypoxia challenge test (HCT) was performed to all volunteers, according to the British Thoracic Society protocol. Ocular coherence tomography angiography (OCT-A) was performed at three time-points (baseline, during HCT and 30' posthypoxia). Macular and peripapillary vessel densities were assessed using the built-in software. To minimize bias, analysis was performed separately in right (OD) and left (OS) eyes. Repeated-measures anova and mean comparison analysis were used as statistical tests (stata v13). Studied population included 30 healthy subjects (14 women), with a mean age of 28.8 ± 4.2 [range 22-37] years. Baseline vessel density increased in hypoxic conditions and subsequently decreased to near-baseline values in posthypoxia conditions. This pattern was observed for both eyes in both parafovea (OD: 55.3 ± 2.3 to 56.7 ± 1.9 to 55.8 ± 1.9, p < 0.05 OS: 56.9 ± 2.1 to 57.9 ± 1.9 to 57.3 ± 1.7, p < 0.05) and peripapillary (OD: 60.5 ± 0.5 to 62.6 ± 0.5 to 60.1 ± 0.4, p < 0.05 OS: 60.4 ± 0.4 to 62.3 ± 0.5 to 60.7 ± 0.4, p < 0.05) areas. To our knowledge, there are no published data specifically addressing mild hypoxia conditions and retinal microvasculature changes, using OCT-A. This pilot study may pave way to better understand vascular responses in disease setting.
Publisher: Wiley
Date: 09-2017
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 06-2017
Publisher: Wiley
Date: 09-2017
Publisher: Frontiers Media SA
Date: 12-06-2019
Publisher: Wiley
Date: 14-09-2016
Publisher: Wiley
Date: 21-08-2016
DOI: 10.1002/DNEU.22331
Abstract: Clustering of acetylcholine receptors (AChR) at the postsynaptic membrane is a crucial step in the development of neuromuscular junctions (NMJ). During development and after denervation, aneural AChR clusters form on the sarcolemma. Recent studies suggest that these receptors are critical for guiding and initiating synaptogenesis. The aim of this study is to investigate the effect of agrin and laminin-1 agents with known AChR clustering activity on NMJ formation and muscle maturation. Primary myoblasts were differentiated in vitro on collagen, laminin or collagen and laminin-coated surfaces in the presence or absence of agrin and laminin. The pretreated cells were then subject to innervation by PC12 cells. The number of neuromuscular junctions was assessed by immunocytochemical co-localization of AChR clusters and the presynaptic marker synaptophysin. Functional neuromuscular junctions were quantitated by analysis of the level of spontaneous as well as neuromuscular blocker responsive contractile activity and muscle maturation was assessed by the degree of myotube striation. Agrin alone did not prime muscle for innervation while a combination of agrin and laminin pretreatment increased the number of neuromuscular junctions formed and enhanced acetylcholine based neurotransmission and myotube striation. This study has direct clinical relevance for treatment of denervation injuries and creating functional neuromuscular constructs for muscle tissue repair.
Publisher: Wiley
Date: 23-09-2015
Publisher: S. Karger AG
Date: 21-10-2016
DOI: 10.1159/000448116
Abstract: b i Purpose: /i /b To determine intra- and inter-rater agreement of anterior lamina cribrosa depth (ALCD) manual measurements using enhanced-depth imaging optical coherence tomography (EDI-OCT). b i Methods: /i /b Observational study. EDI-OCT of the optic nerve head was performed in healthy subjects. ALCD was defined as the perpendicular distance between the line connecting both edges of Bruch's membrane opening and the anterior border of the lamina cribrosa (LC), at the maximum depth point. Two experienced operators performed manual ALCD measurements of the LC independently, and one of the observers performed the same measurements twice. Intra- and inter-rater agreement was evaluated using intraclass correlation coefficients (ICC) and concordance correlation coefficients (CCC) for the right eye vertical (REV) and horizontal (REH) scans and left eye vertical (LEV) and horizontal (LEH) scans. b i Results: /i /b 120 eyes of 61 subjects, with a mean age of 62.1 ± 15.0 years. The mean REV, REH, LEV and LEH ALCD were 456.2 ± 84.3, 444.5 ± 92.2, 436.7 ± 81.6, 427.6 ± 82.7 μm, respectively. ALCD intra- and inter-rater ICC and intra- and inter-rater CCC varied between 0.85-0.95, 0.84-0.93, 0.85-0.95 and 0.84-0.93, respectively. b i Conclusions: /i /b ALCD manual intra- and inter-rater measurements with EDI-OCT showed high agreement. EDI-OCT is a reliable tool for ALCD measurement, which can provide potentially useful information for integrated glaucoma management.
Publisher: SAGE Publications
Date: 26-10-2018
Abstract: The aim of this study is to assess the agreement between manual and automated gonioscopy for iridocorneal angle opening. The research is a cross-sectional observational study. Manual and automated gonioscopy were performed to consecutive patients in a glaucoma clinic. Iridocorneal angle opening grading was performed according to Shaffer’s classification. Automated gonioscopy was performed with NGS-1 automated gonioscope (NIDEK Co., Gamagori, Japan). The automated gonio-photos were graded by two independent observers. Agreement between automated and manual gonioscopy and also among raters was ascertained by Fleiss’ kappa statistic and comparison of area under curve. In total, 88 eyes of 47 subjects were analysed. Mean age was 63 ± 10 years. Twenty eyes (22.7%) were excluded from grading due to poor quality images. Angle closure was detected in 23.4% with dynamic gonioscopy in comparison with 4.3% using automated image grading. The agreement for angle closure diagnosis between dynamic and automated gonioscopy was low (κ = 0.09 ± 0.10 p = 0.18). The area under curve for detecting eyes with angle closure showed poor accuracy between automated and manual methods (area under curve: 0.53 ± 0.05, 95% confidence interval: 0.44–0.62). There was modest inter-rater agreement for angle opening assessment of automated images with Fleiss’ kappa of 0.17 (95% confidence interval: 0.035–0.238). Manual and automated gonioscopy showed only slight agreement for the assessment of iridocorneal angle opening status. Further improvements of the NGS-1 automated gonioscopy and technique are desired for widespread use in a real-life setting.
Publisher: GN1 Genesis Network
Date: 2017
DOI: 10.5935/0004-2749.20170050
Abstract: Studies have suggested that corneal biomechanical properties influence intraocular pressure (IOP) measurements, namely central corneal thickness (CCT) and corneal hysteresis (CH). The present study aimed to investigate the associations of CH and CCT with glaucoma development. We performed a review of the literature and meta-analysis of observational studies (2006-2016) that included both adult glaucoma patients and controls and reported CCT and CH as outcomes. Nineteen studies were conside red eligible, and the mean difference (MD) between groups (patient and control) for both variables was used for statistical analyses. A total of 1,213 glaucoma and 1,055 healthy eyes were studied. Quan titative analysis suggested that CH was significantly lower in the glaucoma group than in the control group (MD=-1.54 mmHg, 95% CI [-1.68, -1.41], P<0.0001). Additionally, CCT was significantly lower in the glaucoma group than in the control group (MD=-8.49 µm, 95% CI [-11.36, -5.62], P<0.001). Corneal properties appear to differ between glaucoma patients and healthy controls. Our results emphasize the importance of corneal biomechanical properties in IOP interpretation and should support further studies on the influence of CH and CCT in glaucoma screening and diagnosis.
Publisher: Wiley
Date: 23-09-2015
Publisher: Wiley
Date: 27-03-2019
DOI: 10.1111/AOS.14103
Publisher: Wiley
Date: 18-03-2020
DOI: 10.1111/AOS.14400
Publisher: Wiley
Date: 25-03-2018
DOI: 10.1111/AOS.13697
Publisher: Wiley
Date: 18-05-2020
DOI: 10.1111/AOS.14467
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 2019
Publisher: Jaypee Brothers Medical Publishing
Date: 08-2016
Publisher: Wiley
Date: 09-2017
Publisher: SAGE Publications
Date: 13-09-2016
DOI: 10.5301/EJO.5000867
Abstract: To investigate the association between anterior lamina cribrosa depth (ALCD), determined with enhanced depth imaging spectral-domain optical coherence tomography (EDI-OCT), and intraocular pressure (IOP) in a healthy Portuguese population. In this cross-sectional observational study conducted between January and July 2015, 2 optic nerve head (ONH)–centered EDI-OCT cross-scans were performed and ALCD was defined as the perpendicular distance between the line connecting both edges of Bruch's membrane opening and the anterior border of the lamina cribrosa at the maximum depth point. A multivariate regression model was performed to assess the association of IOP and ALCD. The studied population included 59 subjects (35 women) with a mean age of 61.7 ± 15.1 years. Mean vertical and horizontal maximum ALCD was 444.5 ± 92.2 μm and 427.6 ± 82.7 μm, respectively. When controlling for age and spherical equivalent, maximum vertical and horizontal ALCD were positively correlated with intraocular pressure (R 2 .20) by 8.58 μm (95% confidence interval [CI] 2.80-14.36 μm p .01) and 8.25 μm (95% CI 2.71-13.78 μm p .01) per mm Hg of IOP, respectively. Our s le of healthy subjects presented a statistically significant positive correlation between IOP and ALCD when controlling for possible confounding factors. These results may trigger further studies to better elucidate the role of IOP in the morphologic and functional dynamics of the ONH.
Publisher: Springer Science and Business Media LLC
Date: 04-07-2020
Publisher: Wiley
Date: 14-09-2016
Publisher: Wiley
Date: 09-2017
Publisher: Springer Science and Business Media LLC
Date: 21-04-2021
No related grants have been discovered for Luis Abegão Pinto.