ORCID Profile
0000-0002-2527-5559
Current Organisations
University of Technology Sydney
,
Charles Darwin University
,
Forensic Science Queensland
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Publisher: Informa UK Limited
Date: 03-2023
Publisher: Springer Science and Business Media LLC
Date: 24-11-2021
DOI: 10.1007/S00414-021-02748-Z
Abstract: In 2019 and 2020, disaster victim identification (DVI) simulations were conducted at the Australian Facility for Taphonomic Experimental Research. Whole and fragmented cadavers were positioned to replicate a building collapse scenario and left to decompose for up to 4 weeks. This study evaluated the utility of the ANDE™ 6C Rapid DNA System and the RapidHIT
Publisher: MDPI AG
Date: 21-10-2021
DOI: 10.3390/FORENSICSCI1030014
Abstract: Advances in forensic biology have increased the options for the collection, s ling, preservation and processing of human remains for DNA-based identification. Combined with a plethora of commercial DNA testing kits that are far more forgiving of inhibited and degraded s les, efficient DNA approaches to post-mortem s les are explored here for DNA-based identification of compromised human remains. Approaches which preserve s le and reduce analytical turnaround times whilst saving resources also have the potential to expedite the identification process, to provide answers to grieving families sooner, or to provide leads in a criminal investigation. Targeting s le types that are minimally-invasive and do not require extensive preparation and testing protocols also has benefit for disaster victim identification (DVI) by facilitating field s ling. We have assessed minimally-invasive and simple to collect s le types compatible with minimal pre-treatment and efficient DNA profiling approaches. Incubating nail, distal phalanges and whole digits in 500 µL of PrepFiler™ Lysis Buffer for 2 h was an efficient and simple method, limiting or removing s le preparation. A reduced 15 min incubation also yielded DNA profiles suggesting a shorter incubation may lyse sufficient DNA. Preservative solutions offer an even simpler process in some cases. Furthermore, the efficient approaches described in this study offer storage solutions and are compatible with backend automated processing. This study will inform further research to develop and optimise efficient protocols. These DNA approaches should not be pursued for every s le more compromised s les may best be submitted to the laboratory for more effective extraction and genotyping.
Publisher: Elsevier BV
Date: 11-2018
DOI: 10.1016/J.FSIGEN.2018.08.016
Abstract: DNA profiling has emerged as the gold standard for the identification of victims in mass disaster events providing an ability to identify victims, reassociate remains and provide investigative leads at a relatively low cost, and with a high degree of discrimination. For the majority of s les, DNA-based identification can be achieved in a fast, streamlined and high-throughput manner. However, a large number of remains will be extremely compromised, characteristic of mass disasters. Advances in technology and in the field of forensic biology have increased the options for the collection, s ling, preservation and processing of s les for DNA profiling. Furthermore, recent developments now allow a vast array of new genetic markers and genotyping techniques to extract as much genetic information from a s le as possible, ensuring that identification is not only accurate but also possible where material is degraded, or limited. Where historically DNA profiling has involved comparison with ante mortem s les or relatives, now DNA profiling can direct investigators towards putative victims or relatives, for comparison through the determination of externally visible characteristics, or biogeographical ancestry. This paper reviews the current and emerging tools available for maximising the recovery of genetic information from post mortem s les in a disaster victim identification context.
Location: Australia
No related grants have been discovered for Jeremy Watherston.