ORCID Profile
0000-0001-7392-3891
Current Organisation
University of Melbourne
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Forestry Sciences | Plant Physiology | Plant Physiology | Cell Metabolism | Forestry Biomass and Bioproducts | Plant Biology | Nutrition And Physiology | Tree Improvement (Selection, Breeding And Genetic Engineering) | Agroforestry |
Forestry | Native forests | Forestry not elsewhere classified | Living resources (flora and fauna) | Integration of Farm and Forestry | Forestry not elsewhere classified
Publisher: Oxford University Press (OUP)
Date: 25-07-2005
DOI: 10.1093/JXB/ERI231
Abstract: Many different techniques have been used for xylem sap collection, but few direct comparisons of techniques have been conducted and few comparisons have been based on comprehensive analyses of xylem sap. Moreover, the suitability of extraction techniques for use on plants grown under water-stress conditions has not been addressed. Xylem sap was extracted from both well-watered and water-stressed Zea mays plants using three different techniques. The main aim was to determine how the extraction method altered the correlations between sap constituents and stomatal conductance in order to determine which relationships change with extraction technique. A 'root pressure' technique was the simplest method of extracting large volumes of sap, but the low sap delivery rates altered the composition of sap. Two pressurization techniques that varied in the position from which sap was collected were tested. The pressurization techniques allowed for the control of delivery rates that influence sap constituent concentrations. The position from which xylem sap was collected on the plant was also found to be important. All three techniques produced consistent correlations between ABA and chloride delivery rates and changes in stomatal conductance, suggesting that each technique could be applied to identify certain putative xylem-borne signals.
Publisher: Elsevier BV
Date: 12-2011
DOI: 10.1016/J.PHYTOCHEM.2011.08.026
Abstract: The glycosylation of lipophilic small molecules produces many important plant secondary metabolites. The majority of these are O-glycosides with relatively fewer occurring as glucose esters of aromatic or aliphatic acids. In particular, monoterpene acid glucose esters have much lower structural ersity and distribution compared to monoterpene glycosides. Nevertheless, there have been over 20 monoterpene acid glucose esters described from trees in the genus Eucalyptus (Myrtaceae) in recent years, all based on oleuropeic acid, menthiafolic acid or both. Here we review all of the glucose esters containing these monoterpenoids identified in plants to date. Many of the compounds contain phenolic aglycones and all contain at least one α,β-unsaturated carbonyl, affording a number of important potential therapeutic reactivities such as anti-tumor promotion, carcinogenesis suppression, and anti-oxidant and anti-inflammatory activities. Additional properties such as cytotoxicity, bitterness, and repellency are suggestive of a role in plant defence, but we also discuss their localization to the exterior of foliar secretory cavity lumina, and suggest they may also protect secretory cells from toxic terpenes housed within these structures. Finally we discuss how the use of a recently developed protocol to isolate secretory cavities in a functional state could be used in conjunction with systems biology approaches to help characterize their biosynthesis and roles in plants.
Publisher: Wiley
Date: 03-12-2022
DOI: 10.1111/NPH.18581
Abstract: The economic and ecologically important genus Eucalyptus is rich in structurally erse specialized metabolites. While some specialized metabolite classes are highly prevalent across the genus, the cyanogenic glucoside prunasin is only produced by c. 3% of species. To investigate the evolutionary mechanisms behind prunasin biosynthesis in Eucalyptus , we compared de novo assembled transcriptomes, together with online resources between cyanogenic and acyanogenic species. Identified genes were characterized in vivo and in vitro . Pathway characterization of cyanogenic Eucalyptus c hora and Eucalyptus yarraensis showed for the first time that the final glucosylation step from mandelonitrile to prunasin is catalyzed by a novel UDP‐glucosyltransferase UGT87. This step is typically catalyzed by a member of the UGT85 family, including in Eucalyptus cladocalyx. The upstream conversion of phenylalanine to mandelonitrile is catalyzed by three cytochrome P450 (CYP) enzymes from the CYP79, CYP706, and CYP71 families, as previously shown. Analysis of acyanogenic Eucalyptus species revealed the loss of different ortholog prunasin biosynthetic genes. The recruitment of UGTs from different families for prunasin biosynthesis in Eucalyptus demonstrates important pathway heterogeneities and unprecedented dynamic pathway evolution of chemical defense within a single genus. Overall, this study provides relevant insights into the tremendous adaptability of these long‐lived trees.
Publisher: Elsevier BV
Date: 08-2009
Publisher: Elsevier BV
Date: 06-2009
DOI: 10.1016/J.PHYTOCHEM.2009.06.004
Abstract: The essential oils extracted from the embedded foliar secretory cavities of many Eucalyptus species are of economic value as pharmaceuticals and fragrance additives. Recent studies have indicated that Eucalyptus secretory cavities may not be exclusively involved in the biosynthesis and storage of essential oils. Therefore, we selected three species upon which to perform an examination of the contents of foliar secretory cavities: Eucalyptus froggattii, E. polybractea and E. globulus. This paper describes the isolation and structural characterization of two non-volatile glucose monoterpene esters, which we have named cuniloside B and froggattiside A, from within the secretory cavities of these species, and shows the presence of these compounds in solvent extracts of the leaves from two other species of Eucalyptus. Both compounds were found in high proportions relative to the essential oils extracted from the leaves. We propose that many other carbohydrate monoterpene esters previously isolated from bulk leaf extracts of various Eucalyptus species may also be localized within the non-volatile fraction of foliar secretory cavities.
Publisher: Elsevier
Date: 2013
Publisher: Elsevier BV
Date: 12-2011
DOI: 10.1016/J.PHYTOCHEM.2011.08.022
Abstract: The cyanogenic glucoside profile of Eucalyptus c hora was investigated in the course of plant ontogeny. In addition to amygdalin, three phenylalanine-derived cyanogenic diglucosides characterized by unique linkage positions between the two glucose moieties were identified in E. c hora tissues. This is the first time that multiple cyanogenic diglucosides have been shown to co-occur in any plant species. Two of these cyanogenic glucosides have not previously been reported and are named eucalyptosin B and eucalyptosin C. Quantitative and qualitative differences in total cyanogenic glucoside content were observed across different stages of whole plant and tissue ontogeny, as well as within different tissue types. Seedlings of E. c hora produce only the cyanogenic monoglucoside prunasin, and genetically based variation was observed in the age at which seedlings initiate prunasin biosynthesis. Once initiated, total cyanogenic glucoside concentration increased throughout plant ontogeny with cyanogenic diglucoside production initiated in saplings and reaching a maximum in flower buds of adult trees. The role of multiple cyanogenic glucosides in E. c hora is unknown, but may include enhanced plant defense and/or a primary role in nitrogen storage and transport.
Publisher: Oxford University Press (OUP)
Date: 28-04-2011
Publisher: American Chemical Society (ACS)
Date: 22-03-2006
DOI: 10.1021/PR050471Q
Abstract: The xylem in plants has mainly been described as a conduit for water and minerals, but emerging evidence also indicates that the xylem contains protein. To study the proteins in xylem sap, we characterized the identity and composition of the maize xylem sap proteome. The composition of the xylem sap proteome in maize revealed proteins related to different phases of xylem differentiation including cell wall metabolism, secondary cell wall synthesis, and programmed cell death. Many proteins were found to be present as multiple isoforms and some of these isoforms are glycosylated. Proteins involved in defense mechanisms were also present in xylem sap and the sap proteins were shown to have antifungal activity in bioassays.
Publisher: Elsevier BV
Date: 10-2015
Publisher: Elsevier BV
Date: 08-2008
Publisher: Elsevier BV
Date: 07-2020
Publisher: Springer Netherlands
Date: 2010
Publisher: Frontiers Media SA
Date: 02-07-2019
Publisher: Elsevier BV
Date: 08-2017
DOI: 10.1016/J.JINSPHYS.2017.06.005
Abstract: Pheromones are chemical compounds used to transmit information between in iduals of the same species. Pheromone composition is influenced by both genetic and environmental factors. Numerous studies, predominately of insects, have demonstrated a role for diet in pheromone expression. The chemical composition of spider web-silk varies with diet and in many species these chemicals are crucial to mate choice processes. Here, we investigated in idual variation in the chemical compounds found on the surface of web-silk of female Argiope keyserlingi, and further explored the degree to which they are influenced by diet, investment in egg sac production and site of collection. We observed variation in the web-based chemical cues both between and within in iduals. Additionally, we found that some of this variation could be explained by diet and gravid status but not by collection site. We discuss our findings in relation to mate choice processes and the costs and benefits of the observed variation in these web-based chemicals.
Publisher: Elsevier BV
Date: 11-2019
Publisher: Elsevier BV
Date: 11-2019
Publisher: Oxford University Press (OUP)
Date: 30-07-2022
DOI: 10.1093/BIOLINNEAN/BLAC089
Abstract: Spider webs are iconic ex les of extended phenotypes that are remarkably plastic across different environments. Orb webs are not only effective traps for capturing prey, but can also provide information to potential mates and, in some cases, potential predators and prey through silk-based chemicals. As with regular phenotypic traits, variability in the properties of spider webs is thought to be mediated by a combination of genetic and environmental effects. Here, we examined variation in several key features of the webs of the orb-weaving spider Argiope keyserlingi across five geographically disparate populations. We documented variation in web architecture and chemical properties of webs collected directly from the field. We then probed the potential for the underlying environmental driver of local insect abundance to explain this variation, by analysing the properties of orb webs constructed by the spiders from these different populations, but under identical laboratory conditions. We found no evidence of variation across populations in the architecture of webs constructed in the laboratory, despite the large geographic distances. Nonetheless, we discovered between population variation in the composition of chemicals found on the surface of silk and in the taxonomic distribution of available prey. Furthermore, there was a positive correlation between the quantity of nitrogenous compounds in web silks and female body condition. When combined, these findings suggest that environmental mechanisms can drive variation in web traits across spider populations.
Publisher: CSIRO Publishing
Date: 2008
DOI: 10.1071/FP07241
Abstract: A protocol for the micropropagation of Eucalyptus polybractea R.T. Baker (blue mallee) using axillary bud proliferation from lignotuber-derived explants is described. Three different ages of plants were used as explant sources: glasshouse-grown seedlings, field-grown saplings, and coppice of field-grown mature lignotubers. Explants from each source initiated successfully and no significant difference was observed for shoot proliferation, rooting success or hardening success between explant sources. Leaf oil quantity and quality for hardened clones transplanted to a field plantation were assessed after 3 months of growth. Ramets of all clones contained high quality oil with over 80% 1,8-cineole. For seedling-derived clones, foliar oil concentrations of ramets were higher than those of the ortets from which they were derived. For sapling and mature lignotuber derived clones the opposite was the case. This suggests that ontogenetic and physiological constraints may be influencing yield in the young ramets. The age of the explant source did not appear to influence the success of micropropagation, and as a result older plants (for which key oil traits are known) can be selected as elite plants for multiplying selected genotypes via micropropagation.
Publisher: Public Library of Science (PLoS)
Date: 15-03-2016
Publisher: Springer Science and Business Media LLC
Date: 29-11-2013
Publisher: Oxford University Press (OUP)
Date: 20-11-2021
Abstract: Research on terpene biosynthesis in the genus Eucalyptus (Myrtaceae) is poorly developed, but recently large numbers of terpene synthase (TPS) genes have been identified. Few of these have been characterized or their expression localized to specific tissues. A prime candidate for detailed examination of TPS gene expression is the bisexual eucalypt flower—composed of male and female reproductive organs, and vegetative tissues that may express different TPS genes. We aimed to characterize and compare the terpene profile and TPS genes expressed in anthers and gynoecia in the high oil-yielding Eucalyptus polybractea R.T. Baker. We hypothesized that gynoecia will produce greater amounts of defensive terpenes, whereas anthers will have a terpene profile that is biased towards a role in pollination. Microscopy of isolated anthers showed them to possess a single, prominent oil gland. Chemical analysis of whole floral structures at different stages of development showed total oil per unit dry mass increased as flower buds expanded, with highest concentrations in mature flower buds just prior to flower opening. The oil profile of gynoecia was dominated by the monoterpene 1,8-cineole, whereas that of isolated anthers were enriched with the monoterpene α-pinene. Through transcriptomic analysis and recombinant protein expression, we were able to identify monoterpene synthases responsible for the different profiles. Synthases for α-pinene and 1,8-cineole were expressed in each tissue type, but the relative expression of the former was higher in anthers. Sequence comparison and site-directed mutagenesis of the α-pinene synthase allowed us to identify amino acids that influence the α-pinene to β-pinene ratio of the product profile. We suggest the terpene constituents of anthers may have multiple roles including attracting pollinators through emission of volatile α-pinene, deterrence of palynivores through emission of volatile 1,8-cineole and adhesion of pollen to pollinators via the release of sticky α-pinene onto the anther surface.
Publisher: CSIRO Publishing
Date: 2002
DOI: 10.1071/FP02027
Abstract: The rare Australian tree Eucalyptus yarraensis Maiden & Cambage is cyanogenic, a quantitative trait potentially indicative of genetic ersity. Cyanogenic plants are capable of releasing cyanide from endogenous cyanide-containing compounds. Cyanide is toxic or deterrent to generalist or non-adapted specialist herbivores. Consequently, cyanogenic plants are afforded an effective means of chemical defense. In this paper we characterize quantitative variation in cyanogenic capability, known as cyanogenic polymorphism, in E. yarraensis for the first time. We show that the cyanogenic glucoside prunasin (R-mandelonitrile-β-D-glucoside) is the only cyanogenic compound in E. yarraensis foliage. We also show that two natural populations of E. yarraensis display extensive intra- and inter-population variation in foliar prunasin concentration. The high prunasin concentrations reported in this paper represent the highest yet recorded for mature eucalypt leaves. The cyanogenic variation could not be attributed to measured physical and chemical parameters, supporting the hypothesis that the variation is genetically based. A preliminary progeny trial also supports this hypothesis, with narrow sense heritability estimated at 1.17 from three half-sibling families. The variation in cyanogenic capability may be a useful tool in the development of a conservation strategy for the species.
Publisher: Oxford University Press (OUP)
Date: 21-06-2010
DOI: 10.1093/JXB/ERQ160
Abstract: Recent reports suggest that early sensing of soil water stress by plant roots and the concomitant reduction in stomatal conductance may not be mediated by root-sourced abscisic acid (ABA), but that other xylem-borne chemicals may be the primary stress signal(s). To gain more insight into the role of root-sourced ABA, the timing and location of the expression of genes for key enzymes involved in ABA biosynthesis in Zea mays roots was measured and a comprehensive analysis of root xylem sap constituents from the early to the later stages of water stress was conducted. Xylem sap and roots were s led from plants at an early stage of water stress when only a reduction in leaf conductance was measured, as well as at later stages when leaf xylem pressure potential decreased. It was found that the majority of ABA biosynthetic genes examined were only significantly expressed in the elongation region of roots at a later stage of water stress. Apart from ABA, sulphate was the only xylem-borne chemical that consistently showed significantly higher concentrations from the early to the later stages of stress. Moreover, there was an interactive effect of ABA and sulphate in decreasing maize transpiration rate and Vicia faba stomatal aperture, as compared to ABA alone. The expression of a sulphate transporter gene was also analysed and it was found that it had increased in the elongation region of roots from the early to the later stages of water stress. Our results support the suggestion that in the early stage of water stress, increased levels of ABA in xylem sap may not be due to root biosynthesis, ABA glucose ester catabolism or pH-mediated redistribution, but may be due to shoot biosynthesis and translocation to the roots. The analysis of xylem sap mineral content and bioassays indicate that the anti-transpirant effect of the ABA reaching the stomata at the early stages of water stress may be enhanced by the increased concentrations of sulphate in the xylem which is also transported from the roots to the leaves.
Publisher: Informa UK Limited
Date: 08-07-2008
DOI: 10.1080/15226510802096242
Abstract: The wastes from some industrial processes and the tailings from gold mining contain elevated concentrations of cyanide, which reacts with iron in the media to form iron cyanide complexes. This research examined the transport and possible metabolism of ferrocyanide by two native Australian trees, blue mallee and sugar gum, and by sorghum. Hydroponic studies using 15N-labeled ferrocyanide showed that both tree species transported ferrocyanide into roots and displayed significant increases in 15N enrichment and concentration with no evidence of phytotoxicity. A subsequent experiment with blue mallee and membrane-transport inhibitors showed that 15N enrichment was significantly inhibited in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone, suggesting that ferrocyanide uptake is mediated partly by H+ -symporters. A study of the time dependence of 15N translocation showed a rapid equilibration of 15N from ferrocyanide in the root of blue mallee, accompanied by a slow increase in shoot 15N, suggestive of the metabolism of ferrocyanide in plant roots. A similar experiment with sorghum showed a more rapid translocation of 15N, suggesting that the transport and/or metabolism of ferrocyanide by roots of this species may differ. The results offer additional incentive for the use of these species as vegetative cover over cyanidation wastes and for cyanide phytoremediation.
Publisher: Oxford University Press (OUP)
Date: 17-12-2009
Abstract: A micropropagation protocol was recently developed for Eucalyptus polybractea R.T. Baker, a commercially important eucalypt grown in short-rotation coppice cultivation and harvested for its foliar 1,8-cineole oil. Micropropagation of elite E. polybractea trees has resulted in selection gains for foliar oil traits, but decreased above-ground biomass accumulation has been observed in clones compared to related half-sibling families. This study aims to use a greenhouse study to investigate if micropropagation induces somaclonal variation that can account for the reduction in above-ground biomass in E. polybractea clones. Secondly, the study aims to compare the coppicing ability of micropropagated clones with related half-sibling seedlings using de-topped plantation-grown saplings. The results of the greenhouse study suggest that micropropagation of E. polybractea induces somaclonal variation that manifests in more mature leaf morphologies such as increased foliar oil concentrations and lower specific leaf area (SLA), attributable to an isobilateral arrangement of increased palisade mesophyll layers. Lower SLA, rather than differences in root allocation, is likely to be a key contributor to the lower relative growth rates observed in early sapling growth of micropropagated clones. In the field study, all micropropagated and seedling-derived E. polybractea saplings coppiced vigorously in the 12 months after de-topping. The coppice growth was so vigorous in the 12 months after de-topping that total above-ground biomass equalled that of the 27-month-old saplings, irrespective of propagation source. The morphological distinction between leaves of micropropagated and seed-derived plants was no longer evident in the coppice regrowth. The results presented here suggest that the micropropagated leaf morphology and the resultant growth reduction is transient and micropropagated plants coppice just as vigorously as seed-derived plants. Therefore, micropropagation is unlikely to detrimentally influence above-ground biomass accumulation beyond the first harvest rotation.
Publisher: Elsevier BV
Date: 07-2017
Publisher: Springer Science and Business Media LLC
Date: 30-08-2006
Publisher: Elsevier BV
Date: 09-2010
DOI: 10.1016/J.CARRES.2010.07.029
Abstract: Short syntheses of cuniloside B and cypellocarpin C, (+)-(R)-oleuropeic acid-containing carbohydrates, are reported. Also disclosed are syntheses of the noreugenin glycosides, undulatoside A and corymbosins K(1) and K(2). Leaf extracts of 28 erse eucalypts revealed cuniloside B to be present in all, and cypellocarpin C to be present in most, of the species examined. The widespread occurrence of these carbohydrate monoterpenoid esters supports their roles in essential oil biosynthesis or mobilization from sites of synthesis to secretory cavity lumena.
Publisher: Springer Science and Business Media LLC
Date: 2010
Publisher: Oxford University Press (OUP)
Date: 02-2013
DOI: 10.1093/AOB/MCT010
Publisher: Oxford University Press (OUP)
Date: 19-07-2018
Abstract: Trees and shrubs from the genus Eucalyptus are characterized by the presence of numerous foliar oil glands that generally house mono- and sesquiterpenes. In some species, glands are also known to house substantial quantities of unrelated secondary metabolites such as volatile, aromatic β-triketones. It is not known if these compounds are co-housed with terpenes or if they are produced in distinct, metabolically specialized glands. We showed that Eucalyptus brevistylis-a species with appreciable foliar quantities of both β-triketones and terpenes-contains two visually distinct gland types in leaves, one that is translucent and the other golden-brown. Gas chromatographic analyses of solvent extracts of the two gland types showed that the translucent glands contain sesquiterpene alcohol cubenols and cubebols (termed 'sesquiterpene glands'), whereas the golden-brown glands contain predominantly the β-triketone conglomerone with lesser amounts of sesquiterpene hydrocarbon caryophyllenes (termed 'triketone glands'). Analysis of leaves from trees of different ages, from young saplings through to advanced age trees, showed a gradual increase in the abundance of sesquiterpene glands relative to triketone glands as plants aged. Such ontogenetic regulation of foliar secondary metabolite concentration appears to be a common feature of Eucalyptus species, albeit at different temporal scales. A similar ontogenetic pattern was observed in ageing leaves, with mature leaves having a higher proportion of sesquiterpene glands than young leaf tips. It is concluded that regulation of the relative abundances of the two gland types with ontogeny likely reflects the different herbivores present at the different life stages of leaves and whole plants. In particular, leaf tips and young plants may be advantaged by deploying higher amounts of insecticidal β-triketones. The concurrent deployment of two metabolically distinct gland types in leaves is a rare phenomenon and a novel finding for myrtaceous trees.
Publisher: Wiley
Date: 07-03-2012
DOI: 10.1111/J.1365-2818.2011.03593.X
Abstract: Multiphoton fluorescence lifetime imaging provides an excellent tool for imaging deep within plant tissues while providing a means to distinguish between fluorophores with high spatial and temporal resolution. Ideal candidates for the application of multiphoton fluorescence lifetime imaging to plants are the embedded secretory cavities found in numerous species because they house complex mixtures of secondary metabolites within extracellular lumina. Previous investigations of this type of structure have been restricted by the use of sectioned material resulting in the loss of lumen contents and often disorganization of the delicate secretory cells thus it is not known if there is spatial segregation of secondary metabolites within these structures. In this paper, we apply multiphoton fluorescence lifetime imaging to investigate the spatial arrangement of metabolites within intact secretory cavities isolated from Eucalyptus polybractea R.T. Baker leaves. The secretory cavities of this species are abundant (up to 10 000 per leaf), large (up to 6 nL) and importantly house volatile essential oil rich in the monoterpene 1,8-cineole, together with an immiscible, non-volatile component comprised largely of autofluorescent oleuropeic acid glucose esters. We have been able to optically section into the lumina of secretory cavities to a depth of ∼80 μm, revealing a unique spatial organization of cavity metabolites whereby the non-volatile component forms a layer between the secretory cells lining the lumen and the essential oil. This finding could be indicative of a functional role of the non-volatile component in providing a protective region of low diffusivity between the secretory cells and potentially autotoxic essential oil.
Publisher: The Royal Society
Date: 30-03-2016
Abstract: Social insects use cuticular hydrocarbons (CHCs) to convey different social signals, including colony or nest identity. Despite extensive investigations, the exact source and identity of CHCs that act as nest-specific identification signals remain largely unknown. Perhaps this is because studies that identify CHC signals typically use organic solvents to extract a single s le from the entire animal, thereby analysing a cocktail of chemicals that may serve several signal functions. We took a novel approach by first identifying CHC profiles from different body parts of ants ( Iridomyrmex purpureus ), then used behavioural bioassays to reveal the location of specific social signals. The CHC profiles of both workers and alates varied between different body parts, and workers paid more attention to the antennae of non-nest-mate and the legs of nest-mate workers. Workers responded less aggressively to non-nest-mate workers if the CHCs on the antennae of their opponents were removed with a solvent. These data indicate that CHCs located on the antennae reveal nest-mate identity and, remarkably, that antennae both convey and receive social signals. Our approach and findings could be valuably applied to chemical signalling in other behavioural contexts, and provide insights that were otherwise obscured by including chemicals that either have no signal function or may be used in other contexts.
Publisher: Public Library of Science (PLoS)
Date: 09-05-2016
Publisher: Springer Netherlands
Date: 2014
Publisher: Public Library of Science (PLoS)
Date: 20-07-2012
Publisher: Elsevier BV
Date: 05-2008
Publisher: Elsevier BV
Date: 10-2007
Publisher: Informa UK Limited
Date: 23-08-2010
DOI: 10.3109/13880209.2010.485619
Abstract: The pharmaceutical alkaloid huperzine A (HupA), currently used in herbal supplements and medicines worldwide, is predominantly sourced from the Chinese lycopod Huperzia serrata (Thunb. ex Murray) Trev. (Lycopodiaceae), which on average contains only 0.08 mg HupA g(-1) dry weight, and is experiencing a rapid decline in China due to over-harvesting. To find a high-yielding, natural source of HupA and/or the related huperzine B (HupB) that could potentially be used as the starting material in a commercial propagation program. We surveyed 17 Huperzia species (15 indigenous to Australia and southeast Asia) for their foliar HupA and HupB concentrations. We also studied intra-specific variation for the huperzines in four species that were available in sufficient numbers, and determined tissue-specific accumulation in larger specimens. HupA was detected in 11 Australasian and southeast Asian species, with eight also containing HupB, albeit at much lower concentrations. A H. elmeri (Herter) Holub plant from the Philippines had one of the highest HupA concentrations recorded (1.01 mg g(-1) dry wt) and it also had the highest HupB content of all plants surveyed (0.34 mg g(-1) dry wt). Intra-specific HupA and HupB concentrations were extremely variable, and at the intra-plant level, reproductive strobili were found to accumulate the highest HupA concentrations. Select Huperzia species from Australia and southeast Asia have potential as the starting material for establishing commercial HupA plantations, but the high intra-specific variability observed suggests that detailed screening is needed to isolate high huperzine-yielding in iduals.
Publisher: Informa UK Limited
Date: 10-2010
Publisher: CSIRO Publishing
Date: 2006
DOI: 10.1071/FP05293
Abstract: Cyanogenesis is the release of cyanide from certain organisms upon tissue disruption. Tissue disruption, such as that caused by folivory, brings cyanogenic glycosides into contact with catabolic enzymes and toxic HCN is subsequently released. The process provides a measure of defence against generalist herbivores. Within the genus Eucalyptus, several species have been identified as cyanogenic and all of these store cyanide exclusively in the form of the cyanogenic glycoside prunasin. Here we report for the first time cyanogenesis in Eucalyptus c hora subsp. humeana L.A.S. Johnson & K.D. Hill. We found that foliage contains at least five different cyanogenic glycosides, three of which were purified and identified (prunasin, sambunigrin and amygdalin). Two natural populations of E. c hora trees were screened for cyanogenesis, and quantitative polymorphism was measured at both sites. Trees varied in their capacity for cyanogenesis from 0.014 to 0.543 mg CN g–1 DW in one population and from 0.011 to 0.371 mg CN g–1 DW in the other. A progeny trial, testing both cyanogenesis and carbon-based defence (namely total phenolics and condensed tannins), was performed with seed sourced from two cyanogenic, open-pollinated maternal trees. Interestingly, the seedlings exhibited markedly lower levels of cyanogenesis and condensed tannins than the adult population, with some in iduals completely lacking one or both of the chemical defences. Total phenolic concentrations, however, were significantly higher in the seedlings than in the parental population from which the seed was sourced. Eucalyptus c hora is relatively unique among cyanogenic trees having multiple foliar cyanogenic glycosides and an apparently marked ontogenetic regulation of cyanogenic capacity.
Publisher: Frontiers Media SA
Date: 10-01-2017
Publisher: Elsevier BV
Date: 05-2013
DOI: 10.1016/J.TPLANTS.2013.01.001
Abstract: Plants are sessile organisms and dependent on deployment of secondary metabolites for their response to biotic and abiotic challenges. A trade-off is envisioned between resources allocated to growth, development, and reproduction and to the biosynthesis, storage, and maintenance of secondary metabolites. However, increasing evidence suggests that secondary metabolites serve auxiliary roles, including functions associated with primary metabolism. In this opinion article, we examine how the costs of plant chemical defense can be offset by multifunctional biosynthesis and the optimization of primary metabolism. These additional benefits may negate the trade-off between primary and secondary metabolism, and provide plants with an innate plasticity required for growth, development, and interactions with their environment.
Publisher: Public Library of Science (PLoS)
Date: 26-08-2019
Publisher: Elsevier BV
Date: 08-2016
Publisher: Cold Spring Harbor Laboratory
Date: 03-03-2020
DOI: 10.1101/2020.03.02.971010
Abstract: The origin of a terrestrial flora in the Ordovician required adaptation to novel biotic and abiotic stressors. Oil bodies, a synapomorphy of liverworts, accumulate secondary metabolites, but their function and development are poorly understood. Oil bodies of Marchantia polymorpha develop within specialized cells as one single large organelle. Here, we show that a CLASS I HOMEODOMAIN LEUCINE-ZIPPER (C1HDZ) transcription factor controls the differentiation of oil body cells in two different ecotypes of the liverwort M. polymorpha , a model genetic system for early ergent land plants. In flowering plants, these transcription factors primarily modulate responses to abiotic stresss including drought. However, loss-of-function alleles of the single ortholog gene, Mp C1HDZ , in M. polymorpha did not exhibit phenotypes associated with abiotic stress. Rather Mp c1hdz mutant plants were more susceptible to herbivory and total plant extracts of the mutant exhibited reduced antibacterial activity. Transcriptomic analysis of the mutant revealed a reduction in expression of genes related to secondary metabolism that was accompanied by a specific depletion of oil body terpenoid compounds. Through time lapse imaging we observed that MpC1HDZ expression maxima precede oil body formation indicating that MpC1HDZ mediates differentiation of oil body cells. Our results indicate that M. polymorpha oil bodies, and MpC1HDZ, are critical for defense against herbivory but not for abiotic stress-tolerance. Thus, C1HDZ genes were co-opted to regulate separate responses to biotic and abiotic stressors in two distinct land plant lineages.
Publisher: Springer Science and Business Media LLC
Date: 07-2012
Publisher: MDPI AG
Date: 16-03-2022
DOI: 10.3390/IJMS23063190
Abstract: Flavonoids are ubiquitous polyphenolic compounds in plants, long recognised for their health-promoting properties in humans. Methylated flavonoids have received increasing attention due to the potential of methylation to enhance medicinal efficacy. Recently, Eucalyptus species with high levels of the O-methylated flavanone pinostrobin have been identified. Pinostrobin has potential commercial value due to its numerous pharmacological and functional food benefits. Little is known about the identity or mode of action of the enzymes involved in methylating flavanones. This study aimed to identify and characterise the methyltransferase(s) involved in the regiospecific methylation of pinostrobin in Eucalyptus and thereby add to our limited understanding of flavanone biosynthesis in plants. RNA-seq analysis of leaf tips enabled the isolation of a gene encoding a flavanone 7-O-methyltransferase (EnOMT1) in Eucalyptus. Biochemical characterisation of its in vitro activity revealed a range of substrates upon which EnOMT1 acts in a regiospecific manner. Comparison to a homologous sequence from a Eucalyptus species lacking O-methylated flavonoids identified critical catalytic amino acid residues within EnOMT1 responsible for its activity. This detailed molecular characterisation identified a methyltransferase responsible for chemical ornamentation of the core flavanone structure of pinocembrin and helps shed light on the mechanism of flavanone biosynthesis in Eucalyptus.
Publisher: Springer Science and Business Media LLC
Date: 29-06-2007
DOI: 10.1007/S00442-007-0787-Y
Abstract: Many studies have shown that similarly aged plants within a species or population can vary markedly in the concentration of defence compounds they deploy to protect themselves from herbivores. Some studies have also shown that the concentration of these compounds can change with development, but no empirical research has mapped such an ontogenetic trajectory in detail. To do this, we grew cyanogenic Eucalyptus yarraensis seedlings from three half-sibling families under constant glasshouse conditions, and followed their foliar cyanogenic glycoside (prunasin) concentration over time for 338 days after sowing (DAS). Plants in all families followed a similar temporal pattern. Plants increased in foliar prunasin concentration from a very low level (10 mug cyanide (CN) equivalents g(-1)) in their first leaves, to a maximum of, on average, 1.2 mg CN g(-1) at about 240 DAS. From 240 to 338 DAS, prunasin concentration gradually decreased to around 0.7 mg CN g(-1). Significant differences between families in maximum prunasin concentration were detected, but none were detected in the time at which this maximum occurred. In parallel with these changes in prunasin concentration, we detected an approximately linear increase in leaf mass per unit leaf area (LMA) with time, which reflected a change from juvenile to adult-like leaf anatomy. When ontogenetic trajectories of prunasin against LMA were constructed, we failed to detect a significant difference between families in the LMA at which maximum prunasin concentration occurred. This remarkable similarity in the temporal and ontogenetic trajectories between in iduals, even from geographically remote families, is discussed in relation to a theoretical model for ontogenetic changes in plant defence. Our results show that ontogeny can constrain the expression of plant chemical defense and that chemical defense changes in a nonlinear fashion with ontogeny.
Publisher: Elsevier BV
Date: 05-2019
Publisher: Elsevier BV
Date: 06-2008
DOI: 10.1016/J.TPLANTS.2008.04.003
Abstract: Chemical signals are important for plant adaptation to water stress. As soils become dry, root-sourced signals are transported via the xylem to leaves and result in reduced water loss and decreased leaf growth. The presence of chemical signals in xylem sap is accepted, but the identity of these signals is controversial. Abscisic acid (ABA), pH, cytokinins, a precursor of ethylene, malate and other unidentified factors have all been implicated in root to shoot signaling under drought. This review describes current knowledge of, and advances in, research on chemical signals that are sent from roots under drought. The contribution of these different potential signals is discussed within the context of their role in stress signaling.
Publisher: Elsevier BV
Date: 07-2002
Start Date: 12-2017
End Date: 12-2020
Amount: $421,854.00
Funder: Australian Research Council
View Funded ActivityStart Date: 01-2010
End Date: 12-2015
Amount: $615,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 2007
End Date: 06-2009
Amount: $261,000.00
Funder: Australian Research Council
View Funded Activity