ORCID Profile
0000-0001-6597-9406
Current Organisations
Instituto de Biotecnología, Universidad Nacional Autónoma de México
,
Università degli Studi di Milano
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Publisher: Elsevier BV
Date: 05-2013
DOI: 10.1016/J.PEP.2013.02.007
Abstract: Five variants of human β-defensins (HBDs) were expressed in Escherichia coli using two vector systems (pET28a(+) and pQE30) with inducible expression by IPTG. The last vector has not been previously reported as an expression system for HBDs. The recombinant peptides were different in their lengths and overall charge. The HBDs were expressed as soluble or insoluble proteins depending on the expression system used, and the final protein yields ranged from 0.5 to 1.6 mg of peptide/g of wet weight cells, with purities higher than 90%. The recombinant HBDs demonstrated a direct correlation between antimicrobial activity and the number of basic charged residues that is, their antimicrobial activity was as follows: HBD3-M-HBD2 > HBD3 = HBD3-M = HB2-KLK > HBD2 when assayed against E. coli, Staphylococcus aureus and Pseudomonas aeruginosa. Interestingly, HBD2 had the best antimicrobial activity against the Mycobacterium tuberculosis strain H37Rv (1.5 μM) and the heterologous tandem peptide, HBD3-M-HBD2, had the best minimal inhibitory concentration (MIC) value (2.7 μM) against a multidrug resistance strain (MDR) of M. tuberculosis, demonstrating the feasibility of the use of HBDs against pathogenic M. tuberculosis reported to be resistant to commercial antibiotics.
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D0SC05017B
Abstract: Generic in silico methodology – CrystalGrower – for simulating crystal habit and nanoscopic surface topology to determine crystallisation free energies.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C4CE01752H
Abstract: A 5-fold cds net and 1D network with a new mode of entanglement were formed from N , N ′-di(3-pyridyl)suberoamide with Cu( ii ) salts.
Publisher: Wiley
Date: 07-04-2021
Abstract: Metal‐organic frameworks (MOFs) are known for their versatile combination of inorganic building units and organic linkers, which offers immense opportunities in a wide range of applications. However, many MOFs are typically synthesized as multiphasic polycrystalline powders, which are challenging for studies by X‐ray diffraction. Therefore, developing new structural characterization techniques is highly desired in order to accelerate discoveries of new materials. Here, we report a high‐throughput approach for structural analysis of MOF nano‐ and sub‐microcrystals by three‐dimensional electron diffraction (3DED). A new zeolitic‐imidazolate framework (ZIF), denoted ZIF‐EC1, was first discovered in a trace amount during the study of a known ZIF‐CO 3 ‐1 material by 3DED. The structures of both ZIFs were solved and refined using 3DED data. ZIF‐EC1 has a dense 3D framework structure, which is built by linking mono‐ and bi‐nuclear Zn clusters and 2‐methylimidazolates (mIm − ). With a composition of Zn 3 (mIm) 5 (OH), ZIF‐EC1 exhibits high N and Zn densities. We show that the N‐doped carbon material derived from ZIF‐EC1 is a promising electrocatalyst for oxygen reduction reaction (ORR). The discovery of this new MOF and its conversion to an efficient electrocatalyst highlights the power of 3DED in developing new materials and their applications.
Publisher: Wiley
Date: 07-04-2021
Publisher: Wiley
Date: 29-09-2008
Publisher: International Union of Crystallography (IUCr)
Date: 12-09-2013
DOI: 10.1107/S0108767313020655
Abstract: Nets in which different vertices have identical barycentric coordinates ( i.e. have collisions) are called unstable. Some such nets have automorphisms that do not correspond to crystallographic symmetries and are called non-crystallographic. Ex les are given of nets taken from real crystal structures which have embeddings with crystallographic symmetry in which colliding nodes either are, or are not, topological neighbors (linked) and in which some links coincide. An ex le is also given of a crystallographic net of exceptional girth (16), which has collisions in barycentric coordinates but which also has embeddings without collisions with the same symmetry. In this last case the collisions are termed unforced .
Publisher: American Chemical Society (ACS)
Date: 03-12-2019
Publisher: Elsevier BV
Date: 03-2013
DOI: 10.1016/J.PEPTIDES.2013.01.009
Abstract: Peptide de13a was previously purified from the venom of the worm-hunting cone snail Conus delessertii from the Yucatán Channel, México. This peptide has eight cysteine (Cys) residues in the unique arrangement C-C-C-CC-C-C-C, which defines the cysteine framework XIII ("-" represents one or more non-Cys residues). Remarkably, δ-hydroxy-lysine residues have been found only in conotoxin de13a, which also contains an unusually high proportion of hydroxylated amino acid residues. Here, we report the cDNA cloning of the complete precursor De13.1 of a related peptide, de13b, which has the same Cys framework and inter-Cys spacings as peptide de13a, and shares high protein/nucleic acid sequence identity (87%/90%) with de13a, suggesting that both peptides belong to the same conotoxin gene superfamily. Analysis of the signal peptide of precursor De13.1 reveals that this precursor belongs to a novel conotoxin gene superfamily that we chose to name gene superfamily G. Thus far superfamily G only includes two peptides, each of which contains the same, distinctive Cys framework and a high proportion of amino acid residues with hydroxylated side chains.
Publisher: American Chemical Society (ACS)
Date: 07-05-2018
Location: Mexico
No related grants have been discovered for Davide M. Proserpio.