ARDC Research Link Australia

ORCID Profile
Orcid icon. 0000-0001-9055-1073

Current Organisations
University of Helsinki , Karolinska Institutet , Texas A&M University at Qatar , Univerzitet u Beogradu Institut za Fiziku

Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.

Publications

Publication

Genomic evidence for contrasting patterns of host‐associated genetic differentiation across shared host‐plant species in leaf‐ and bud‐galling sawflies

Publisher: Wiley

Date: 23-01-2023

DOI: 10.1111/MEC.16844

Abstract: Resource specialization and ecological speciation arising through host‐associated genetic differentiation (HAD) are frequently invoked as an explanation for the high ersity of plant‐feeding insects and other organisms with a parasitic lifestyle. While genetic studies have demonstrated numerous ex les of HAD in insect herbivores, the rarity of comparative studies means that we still lack an understanding of how deterministic HAD is, and whether patterns of host shifts can be predicted over evolutionary timescales. We applied genome‐wide single nucleotide polymorphism and mitochondrial DNA sequence data obtained through genome resequencing to define species limits and to compare host‐plant use in population s les of leaf‐ and bud‐galling sawflies (Hymenoptera: Tenthredinidae: Nematinae) collected from seven shared willow (Salicaceae: Salix ) host species. To infer the repeatability of long‐term cophylogenetic patterns, we also contrasted the phylogenies of the two galler groups with each other as well as with the phylogeny of their Salix hosts estimated based on RADseq data. We found clear evidence for host specialization and HAD in both of the focal galler groups, but also that leaf gallers are more specialized to single host species compared with most bud gallers. In contrast to bud gallers, leaf gallers also exhibited statistically significant cophylogenetic signal with their Salix hosts. The observed discordant patterns of resource specialization and host shifts in two related galler groups that have radiated in parallel across a shared resource base indicate a lack of evolutionary repeatability in the focal system, and suggest that short‐ and long‐term host use and ecological ersification in plant‐feeding insects are dominated by stochasticity and/or lineage‐specific effects.

Publication

Label-free third harmonic generation imaging and quantification of lipid droplets in live filamentous fungi

Publisher: Springer Science and Business Media LLC

Date: 05-11-2022

DOI: 10.1038/S41598-022-23502-4

Abstract: We report the utilization of Third-Harmonic Generation microscopy for label-free live cell imaging of lipid droplets in the hypha of filamentous fungus Phycomyces blakesleeanus . THG microscopy images showed bright spherical features dispersed throughout the hypha cytoplasm in control conditions and a transient increase in the number of bright features after complete nitrogen starvation. Colocalization analysis of THG and lipid-counterstained images disclosed that the cytoplasmic particles were lipid droplets. Particle Size Analysis and Image Correlation Spectroscopy were used to quantify the number density and size of lipid droplets. The two analysis methods both revealed an increase from 16 × 10 −3 to 23 × 10 −3 lipid droplets/µm 2 after nitrogen starvation and a decrease in the average size of the droplets (range: 0.5–0.8 µm diameter). In conclusion, THG imaging, followed by PSA and ICS, can be reliably used for filamentous fungi for the in vivo quantification of lipid droplets without the need for labeling and/or fixation. In addition, it has been demonstrated that ICS is suitable for THG microscopy.

Publication