ORCID Profile
0000-0001-6631-6321
Current Organisation
Nanyang Technological University
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Publisher: Springer Science and Business Media LLC
Date: 17-06-2015
DOI: 10.1038/SREP11425
Abstract: Despite significant advancements over the years, there remains an urgent need for low cost diagnostic approaches that allow for rapid, reliable and sensitive detection of malaria parasites in clinical s les. Our previous work has shown that magnetic resonance relaxometry (MRR) is a potentially highly sensitive tool for malaria diagnosis. A key challenge for making MRR based malaria diagnostics suitable for clinical testing is the fact that MRR baseline fluctuation exists between in iduals, making it difficult to detect low level parasitemia. To overcome this problem, it is important to establish the MRR baseline of each in idual while having the ability to reliably determine any changes that are caused by the infection of malaria parasite. Here we show that an approach that combines the use of microfluidic cell enrichment with a saponin lysis before MRR detection can overcome these challenges and provide the basis for a highly sensitive and reliable diagnostic approach of malaria parasites. Importantly, as little as 0.0005% of ring stage parasites can be detected reliably, making this ideally suited for the detection of malaria parasites in peripheral blood obtained from patients. The approaches used here are envisaged to provide a new malaria diagnosis solution in the near future.
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D3LC00379E
Publisher: Cold Spring Harbor Laboratory
Date: 04-05-2023
DOI: 10.1101/2023.05.03.539013
Abstract: The growing interest in regenerative medicine has opened new avenues for novel cell therapies using stem cells. Bone Marrow Aspirate (BMA) is an important source of stromal mesenchymal stem cells (MSCs). Conventional MSC harvesting from BMA relies on archaic centrifugation methods, often leading to poor yield due to osmotic stress, high centrifugation force, convoluted workflow, and long experimental time (∼ 2 – 3 hours). To address these issues, we have developed a scalable microfluidic technology based on Deterministic Lateral Displacement (DLD) for MSC isolation. This passive, label-free cell sorting method capitalizes on the morphological differences between MSCs and blood cells (leukocytes and RBCs) for effective separation using an inverted L-shaped pillar array. To improve throughput, we developed a novel portable multiplexed DLD system that can process 2.5 mL of raw BMA in 20 ± 5 minutes, achieving a 2-fold increase in MSC recovery compared to centrifugation methods. Taken together, we envision the developed DLD platform will enable fast and efficient isolation of MSCs from BMA for effective downstream cell therapy in clinical settings.
Publisher: Springer Science and Business Media LLC
Date: 02-07-2021
DOI: 10.1038/S42003-021-02295-8
Abstract: Sialyl-Lewis x (sLe x , CD15s) is a tetra-saccharide on the surface of leukocytes required for E-selectin-mediated rolling, a prerequisite for leukocytes to migrate out of the blood vessels. Here we show using flow cytometry that sLe x expression on basophils and mast cell progenitors depends on fucosyltransferase 6 ( FUT6 ). Using genetic association data analysis and qPCR, the cell type-specific defect was associated with single nucleotide polymorphisms (SNPs) in the FUT6 gene region (tagged by rs17855739 and rs778798), affecting coding sequence and/or expression level of the mRNA. Heterozygous in iduals with one functional FUT6 gene harbor a mixed population of sLe x+ and sLe x- basophils, a phenomenon caused by random monoallelic expression (RME). Microfluidic assay demonstrated FUT6 -deficient basophils rolling on E-selectin is severely impaired. FUT6 null alleles carriers exhibit elevated blood basophil counts and a reduced itch sensitivity against insect bites. FUT6 -deficiency thus d ens the basophil-mediated allergic response in the periphery, evident also in lower IgE titers and reduced eosinophil counts.
Publisher: Springer Science and Business Media LLC
Date: 12-02-2013
DOI: 10.1038/SREP01259
Publisher: Elsevier BV
Date: 07-2021
Location: United States of America
No related grants have been discovered for Han Wei Hou.