ORCID Profile
0000-0001-7124-3542
Current Organisation
Hong Kong University of Science and Technology
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Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2BM00705C
Abstract: To overcome the endosomal barrier, we attached a fusogenic peptide (L17E) onto peptide self-assembled disks, which mimicked the functional subunits of the virus capsid and improved transfection efficiency.
Publisher: Wiley
Date: 06-10-2021
Abstract: Aromatic residues are widely used as building blocks for driving self‐assemblies in natural and designer biomaterials. The noncovalent interactions involving aromatic rings determine proteins’ structure and biofunction. Here, we studied the effects of changes in the proximity of the aromatic rings in a self‐assembling peptide for modulating interactions involving the aromatic residues. By changing the distance between the aromatic ring and peptide backbone and replacing the side chain with a sulfur atom, we altered the nanostructures and gene transfection efficiency of peptide‐DNA co‐assemblies. This study demonstrates the significance of subtle alterations in aromatic interactions and facilitates deeper understanding of the aromatic‐involving interactions.
Publisher: American Chemical Society (ACS)
Date: 21-06-2022
Abstract: Broadening the applicable tools for mRNA delivery provides more flexibility in research and those proven effective and safe can potentially be translated for clinical use. We report here a 27-amino acid peptide sequence mimicking the viral capsid protein, termed pepMAX, capable of co-assembling with mRNA into 100-150 nm nanostructures for efficient transfection of multiple cell lines. The mRNA loading and N/P ratio have been systematically optimized for each cell line. In HeLa, HEK293, and SKNMC, the transfection attained (>80%) is comparable with that of commercially available vectors Lipofectamine MessengerMAX
Publisher: MDPI AG
Date: 09-07-2019
DOI: 10.3390/LIFE9030059
Abstract: The discovery of the genetic roots of various human diseases has motivated the exploration of different exogenous nucleic acids as therapeutic agents to treat these genetic disorders (inherited or acquired). However, the physicochemical properties of nucleic acids render them liable to degradation and also restrict their cellular entrance and gene translation/inhibition at the correct cellular location. Therefore, gene condensation rotection and guided intracellular trafficking are necessary for exogenous nucleic acids to function inside cells. Diversified cationic formulation materials, including natural and synthetic lipids, polymers, and proteins eptides, have been developed to facilitate the intracellular transportation of exogenous nucleic acids. The chemical properties of different formulation materials determine their special features for nucleic acid delivery, so understanding the property–function correlation of the formulation materials will inspire the development of next-generation gene delivery carriers. Therefore, in this review, we focus on the chemical properties of different types of formulation materials and discuss how these formulation materials function as protectors and cellular pathfinders for nucleic acids, bringing them to their destination by overcoming different cellular barriers.
Publisher: American Chemical Society (ACS)
Date: 17-02-2023
No related grants have been discovered for Rong Ni.