ORCID Profile
0000-0002-3033-0268
Current Organisation
Syiah Kuala University
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Publisher: F1000 Research Ltd
Date: 08-04-2022
DOI: 10.12688/F1000RESEARCH.110305.1
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10 13 PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10 13 PFU/mL to 10 9 PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.
Publisher: F1000 Research Ltd
Date: 15-06-2023
DOI: 10.12688/F1000RESEARCH.110305.2
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10 13 PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10 13 PFU/mL to 10 9 PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.
Publisher: F1000 Research Ltd
Date: 12-04-2023
DOI: 10.12688/F1000RESEARCH.109676.2
Abstract: Background : The decrease of immunity acquired from COVID-19 vaccines is a potential cause of breakthrough infection. Understanding the dynamics of immune responses of vaccine-induced antibodies post-vaccination is important. This study aimed to measure the level of anti-SARS-CoV-2 receptor-binding domain (RBD) total antibody in in iduals at different time points upon the receipt of the second dose of CoronaVac vaccine, as well as evaluate the plausible associated factors. Methods : A cross-sectional study was conducted among CoronaVac-vaccinated residents in Banda Aceh, Indonesia. The level of anti-SARS-CoV-2 RBD total antibody was measured using Elecsys immunoassay. A set of standardized and validated questionnaires were used to assess the demographics and other associated factors. Results : Our results showed waning anti-SARS-CoV-2 RBD total antibody titres over time post-vaccination. Compared to s les of the first month post-vaccination, the antibody titres were significantly lower than those of five-months (mean 184.6 vs. 101.8 U/mL, p = 0.009) and six-months post-vaccination (mean 184.6 vs. 95.59 U/mL, p = 0.001). This suggests that the length of time post-vaccination was negatively correlated with titre of antibody. A protective level of antibody titres (threshold of 15 U/mL) was observed from all the s les vaccinated within one to three months however, only 73.7% and 78.9% of the sera from five- and six-months possessed the protective titres, respectively. The titre of antibody was found significantly higher in sera of in iduals having a regular healthy meal intake compared to those who did not (mean 136.7 vs. 110.4 U/mL, p = 0.044), including in subgroup analysis that included those five to six months post-vaccination only (mean 79.0 vs. 134.5 U/mL, p = 0.009). Conclusions : This study provides insights on the efficacy of CoronaVac vaccine in protecting in iduals against SARS-CoV-2 infection over time, which may contribute to future vaccination policy management to improve and prolong protective strategy.
Publisher: F1000 Research Ltd
Date: 10-03-2022
DOI: 10.12688/F1000RESEARCH.109676.1
Abstract: Background : The decrease of immunity acquired from COVID-19 vaccines is a potential cause of breakthrough infection. Understanding the dynamics of immune responses of vaccine-induced antibodies post-vaccination is important. This study aimed to measure the level of neutralizing antibody (NAb) anti-SRBD in in iduals at different time points upon the receipt of the second dose of CoronaVac vaccine, as well as evaluate the plausible associated factors. Methods : A cross-sectional study was conducted among CoronaVac-vaccinated residents in Banda Aceh, Indonesia. The level of NAb titre was measured using Elecsys Anti-SARS-CoV-2 S immunoassay. A set of standardized and validated questionnaires were used to assess the demographics and other plausible associated factors. Results : Our results showed waning SARS-Cov-2 NAb titres over time post-vaccination. Compared to s les of the first month post-vaccination, the levels of NAb titres were significantly lower than those of five-months (mean 184.6 vs. 101.8 IU/mL, p = 0.009) and six-months post-vaccination (mean 184.6 vs. 95.59 IU/mL, p = 0.001). This suggests that the length of time post-vaccination was negatively correlated with antibody anti-SRBD titre. A protective level of NAbs titres (threshold of 15 IU/mL) was observed from all the s les vaccinated within one to three months however, only 73.7% and 78.9% of the sera from five- and six-months possessed the protective titres against SARS-CoV-2, respectively. The titre of NAb anti-SRBD was found significantly higher in sera of in iduals having a regular healthy meal intake compared to those who did not (mean 136.7 vs. 110.4 IU/mL, p = 0.044), including in subgroup analysis that included those five to six months post-vaccination only (mean 79.0 vs. 134.5 IU/mL, p = 0.009). Conclusions : This study provides insights on the efficacy of CoronaVac vaccine in protecting in iduals against SARS-CoV-2 infection over time, which may contribute to future vaccination policy management to improve and prolong protective strategy.
Publisher: MDPI AG
Date: 14-09-2022
DOI: 10.3390/SU141811537
Abstract: The controlled environment room, called an isolation room, has become a must have for medical facilities, due to the spreading of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), to isolate the high risk infected patients. To avoid the transmission of the virus through airborne routes, guidelines were published by the government and the association. A medical facility must comply with this document for high-risk patient treatment. A full-scale N class isolation room was built at Syiah Kuala University to investigate the performance in terms of the controller, temperature, pressure, humidity, and energy consumption. The isolation room was equipped with a proper capacity heating, ventilating, and air conditioning (HVAC) system, which consisted of an air conditioning compressor and a negative pressure generator (NPG), and its installation was ensured to fulfil the guidelines. Since the current NPG was controlled manually, a computer-based control system was designed, implemented, and compared with the manual control. The results showed that the computer-based control outputs better stability of pressure and electric power. For that reason, a computer-based control was chosen in the real case. To investigate the performance of the isolation room, a 24 h experiment was carried out under different parameter setups. The results showed that improvement of the control strategy for temperature and humidity is still necessary. The energy consumption during the activation of the NPG for the recommended negative pressure was slightly different. An additional piece of equipment to absorb the heat from the exhaust air would be promising to improve the energy efficiency.
No related grants have been discovered for Rudi Kurniawan.