ORCID Profile
0000-0001-5882-9573
Current Organisations
Bangabandhu Sheikh Mujibur Rahman Agricultural University
,
University of Nottingham
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Publisher: Oxford University Press (OUP)
Date: 28-12-2019
DOI: 10.1111/LAM.13256
Abstract: Bacterial communities strongly influence the digestion, health and immune status of fish. This study investigates the microbial distribution of the anterior, middle and distal gut sections of three economically important carp species in Bangladesh, rohu, catla and mrigal (commonly known as Indian major carps), using 16S rRNA-based Illumina sequencing technology. The alpha- ersity measurement with one-way ANOVA indicated high species richness, Shannon and Simpson indices in the middle and distal gut, while the anterior gut of IMCs had the lowest ersity. At the phylum level, there was high abundance of Proteobacteria in the GITs of rohu and mrigal, whereas Fusobacteria was dominant in the anterior and middle guts of catla. At the genus level, erse microbial communities were identified across the three GIT sections, with six indicator genera found in rohu, catla and mrigal, as revealed by linear discriminant analysis (LDA) at a 0·05 level of significance. Of the 218 genera identified, only 33 were common across the anterior, middle and distal guts of all three species. Bacterial ersity was significantly higher (P < 0·05) in mrigal, followed by catla and rohu, respectively. Alongside the common bacteria Aeromonas, Enterobacter and Serratia, the overwhelming abundance of Cetobacterium, Shewanella and Plesiomonas warrants further investigation. SIGNIFICANCE AND IMPACT OF THE STUDY: This study investigates the microbial communities of the gastrointestinal tracts (GITs) of three Indian major carp (IMC) species-rohu, catla and mrigal, obtained from a polyculture pond under the same feeding regime. Diverse microbial communities were found, with significantly different relative abundances and ersities of phyla and genera. The results provide valuable information on GIT microbial communities that may be useful for nutrition and health management in IMCs.
Publisher: Elsevier BV
Date: 04-2020
Publisher: Oxford University Press (OUP)
Date: 29-04-2019
DOI: 10.1111/JAM.14257
Abstract: This study conducted bacterial community, virulence and antibiogram profiling inside the hindgut and skin of freshly caught hilsa fish and those sold at markets. The results of 16S rRNA-based high-throughput sequencing showed a higher number of bacterial genera in marketed fish s les than in fresh fish s les. The total operational taxonomic units, genus counts and ersity index were significantly higher (P > 0·05) in marketed fish, which also had abundant pathogenic bacterial groups. Skin s les had a lower profusion of pathogenic bacteria than gut s les. A total of 52 bacterial isolates from nine species were identified in this study, of which 25 were from a Chittagong market and 22 were from a Dhaka market, whereas only five were from fresh hilsa. The polymerase chain reaction lification of 12 species-specific virulence genes in the 52 isolates, namely, aer, hly, chxA, toxB, rtxC, sfa, uge, norB, trx, toxA, ipaH, sigA and coa, indicated a high number of positive s les containing Vibrio cholerae, Aeromonas spp., Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus. Antibiogram profiling of these bacteria against 10 commercial antibiotics showed high-resistance patterns of the isolates against sulfamethoxazole, kanamycin, neomycin, icillin and tetracycline. The results reveal the spread of multidrug-resistant bacteria in hilsa fish marketed for human consumption in Bangladesh. This study highlights the risk of spreading environmentally and clinically pathogenic bacteria in fish sold for human consumption in Bangladesh. Such bacteria come from aquatic pollution and poor handling, storage and transportation practices that may predispose fish to major outbreaks of infectious and waterborne diseases.
Publisher: Oxford University Press (OUP)
Date: 10-2022
DOI: 10.1111/LAM.13739
Abstract: The present study aimed to characterize and compare the skin and gut microbial communities of rohu at various post-harvest stages of consumption using quantitative real-time polymerase chain reaction and 16S rRNA-based licon sequencing. Real-time PCR lification detected higher copy numbers for coliform bacteria—Escherichia coli, Salmonella enterica and Shigella spp. in the marketed fish—compared to fresh and frozen s les. The 16S rRNA data revealed higher alpha ersity measurements in the skin of fish from different retail markets of Dhaka city. Beta ordination revealed distinct clustering of bacterial OTUs for the skin and gut s les from three different groups. At the phylum level, Proteobacteria was most abundant in all groups except the Fusobacteria in the control fish gut. Although Aeromonas was found ubiquitous in all types of s les, erse bacterial genera were identified in the marketed fish s les. Nonetheless, low species richness was observed for the frozen fish. Most of the differentially abundant bacteria in the skin s les of marketed fish are opportunistic human pathogens enriched at different stages of postharvest handling and processing. Therefore, considering the microbial contamination in the aquatic environment in Bangladesh, post-harvest handling should be performed with proper methods and care to minimize bacterial transmission into fish.
Publisher: Wiley
Date: 18-11-2020
DOI: 10.1111/JFD.13304
Location: Bangladesh
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for A.Q.M. Robiul Kawser.