ORCID Profile
0000-0001-5021-1234
Current Organisations
King's College London
,
Universidade Estadual do Oeste do Paraná
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Publications
A consensus protocol for functional connectivity analysis in the rat brain
Publisher: Springer Science and Business Media LLC
Date: 27-03-2023
CSF1R inhibitor JNJ-40346527 attenuates microglial proliferation and neurodegeneration in P301S mice
Publisher: Oxford University Press (OUP)
Date: 26-08-2019
DOI: 10.1093/BRAIN/AWZ241
Abstract: Microglia have been implicated in amyloid beta-induced neuropathology, but their role in tau-induced neurodegeneration remains unclear. Mancuso et al. report that blockade of microglial proliferation by CSF1R inhibitor JNJ-40346527 modifies brain inflammation and ameliorates disease progression in P301S tauopathy mice. CSF1R inhibition may have therapeutic potential in tau-mediated neurodegenerative diseases.
FUNCTIONAL PROFILE OF ACTIVE OLDER ADULTS WITH LOW BACK PAIN, ACCORDING TO THE ICF
Publisher: FapUNIFESP (SciELO)
Date: 08-2016
DOI: 10.1590/1517-869220162204159647
Abstract: ABSTRACT Introduction: The International Classification of Functioning, Disability and Health (ICF) considers multiples aspects of functionality. It is believed that this tool can help to classify the functionality of older adults with low back pain (LBP) . Objectives: To describe the functionality of active older adults with LBP according to the ICF. Methods: A transversal study was conducted using the brief ICF core set for low back pain, to establish functional profiles of 40 older adults. The ICF categories were considered valid when ≥20% of participants showed some disability. Results: Thirty-two of the 35 categories of the brief ICF core set could be considered representative of the s le. Conclusion: The brief ICF core set for LBP results demonstrated that this classification system is representative for describing the functional profile of the s le.
Neuroprotection by remote ischemic conditioning in the setting of acute ischemic stroke: a preclinical two-centre study
Publisher: Springer Science and Business Media LLC
Date: 09-10-2020
DOI: 10.1038/S41598-020-74046-4
Abstract: Reperfusion is the only existing strategy for patients with acute ischemic stroke, however it causes further brain damage itself. A feasible therapy targeting reperfusion injury is remote ischemic conditioning (RIC). This was a two-centre, randomized, blinded international study, using translational imaging endpoints, aimed to examine the neuroprotective effects of RIC in ischemic stroke model. 80 male rats underwent 90-min middle cerebral artery occlusion. RIC consisted of 4 × 5 min cycles of left hind limb ischemia. The primary endpoint was infarct size measured on T2-weighted MRI at 24 h, expressed as percentage of the area-at-risk. Secondary endpoints were: hemispheric space-modifying edema, infarct growth between per-occlusion and 24 h MRI, neurofunctional outcome measured by neuroscores. 47 rats were included in the analysis after applying pre-defined inclusion criteria. RIC significantly reduced infarct size (median, interquartile range: 19% [8% 32%] vs control: 40% [17% 59%], p = 0.028). This effect was still significant after adjustment for apparent diffusion coefficient lesion size in multivariate analysis. RIC also improved neuroscores (6 [3 8] vs control: 9 [7 11], p = 0.032). Other secondary endpoints were not statistically different between groups. We conclude that RIC in the setting of acute ischemic stroke in rats is safe, reduces infarct size and improves functional recovery.
Tissue Optimisation Strategies for High Quality Ex Vivo Diffusion Imaging
Publisher: Cold Spring Harbor Laboratory
Date: 14-12-2021
DOI: 10.1101/2021.12.13.472113
Abstract: Ex vivo diffusion imaging can be used to study healthy and pathological tissue microstructure in the rodent brain with microscopic resolution, providing a link between in vivo MRI and ex vivo microscopy techniques. A major challenge for the successful acquisition of ex vivo diffusion imaging data however are changes in the relaxivity and diffusivity of brain tissue following perfusion fixation. In this study we address this question by examining the combined effects of tissue preparation factors that influence image quality, including tissue rehydration time, fixative concentration and contrast agent concentration. We present an optimisation strategy combining these factors to manipulate the T1 and T2 of fixed tissue and maximise signal-to-noise ratio (SNR) efficiency. Applying this strategy in the rat brain resulted in a doubling of SNR and an increase in SNR per unit time by 135% in grey matter and 88% in white matter. This enabled the acquisition of excellent quality high-resolution (78 µm isotropic voxel size) diffusion data in less than 4 days, with a b -value of 4000 s/mm 2 , 30 diffusion directions and a field of view of 40 × 13 × 18 mm, using a 9.4 Tesla scanner with a standard 39 mm volume coil and a 660 mT/m 114 mm gradient insert. It was also possible to achieve comparable data quality for a standard resolution (150 µm) diffusion dataset in 2 1 / 4 hours. In conclusion, the optimisation strategy presented here may be used to improve signal quality, increase spatial resolution and/or allow faster acquisitions in preclinical ex vivo diffusion MRI experiments.
Tissue optimization strategies for high‐quality ex vivo diffusion imaging
Publisher: Wiley
Date: 04-12-2022
DOI: 10.1002/NBM.4866
Abstract: Ex vivo diffusion imaging can be used to study healthy and pathological tissue microstructure in the rodent brain with high resolution, providing a link between in vivo MRI and ex vivo microscopy techniques. Major challenges for the successful acquisition of ex vivo diffusion imaging data however are changes in the relaxivity and diffusivity of brain tissue following perfusion fixation. In this study we address this question by examining the combined effects of tissue preparation factors that influence signal‐to‐noise ratio (SNR) and consequently image quality, including fixative concentration, contrast agent concentration and tissue rehydration time. We present an optimization strategy combining these factors to manipulate the and of fixed tissue and maximize SNR efficiency. We apply this strategy in the rat brain, for a diffusion‐weighted spin echo protocol with TE = 27 ms on a 9.4 T scanner with a 39 mm volume coil and 660 mT/m 114 mm gradient insert. We used a reduced fixative concentration of 2% paraformaldehyde (PFA), rehydration time more than 20 days, 15 mM Gd‐DTPA in perfusate and TR 250 ms. This resulted in a doubling of SNR and an increase in SNR per unit time of 135% in cortical grey matter and 88% in white matter compared with 4% PFA and no contrast agent. This improved SNR efficiency enabled the acquisition of excellent‐quality high‐resolution (78 m isotropic voxel size) diffusion data with b = 4000 s/mm , 30 diffusion directions and a field of view of mm 3 in less than 4 days. It was also possible to achieve comparable data quality for a standard resolution (150 m) diffusion dataset in h. In conclusion, the tissue optimization strategy presented here may be used to improve SNR, increase spatial resolution and/or allow faster acquisitions in preclinical ex vivo diffusion MRI experiments.
Effect of photobiomodulation in the patellofemoral pain syndrome; randomized clinical trial in young women
Publisher: Elsevier BV
Date: 04-2021
Related Organisations
King's College London
Location: United Kingdom of Great Britain and Northern Ireland
Universidade De São Paulo Faculdade De Medicina De Ribeirão Preto
Location: Brazil
Related Funding Activities
No related grants have been discovered for diana Cash.