ORCID Profile
0000-0003-4876-2410
Current Organisations
University of Barcelona
,
Hospital Clínic de Barcelona
,
Universitat de Barcelona
,
Institut d'Investigacions Biomèdiques August Pi i Sunyer
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: BMJ
Date: 19-09-2008
Publisher: Wiley
Date: 07-1998
DOI: 10.1002/(SICI)1098-2795(199807)50:3<345::AID-MRD11>3.0.CO;2-3
Abstract: Antibiotic resistance challenges public health on many fronts, and it is increasingly clear that it must be addressed in the environment to control emerging resistance and infections in humans and animals. Here, we outline how the US Centers for Disease Control and Prevention is addressing antibiotic resistance in the environment.
Publisher: Impact Journals, LLC
Date: 08-10-2019
Publisher: Oxford University Press (OUP)
Date: 14-02-2019
DOI: 10.1002/STEM.2966
Abstract: When considering the clinical applications of autologous cell replacement therapy of human induced pluripotent stem cells (iPSC)-derived cells, there is a clear need to better understand what the immune response will be before we embark on extensive clinical trials to treat or model human disease. We performed a detailed assessment comparing human fibroblast cell lines (termed F1) reprogrammed into human iPSC and subsequently differentiated back to fibroblast cells (termed F2) or other human iPSC-derived cells including neural stem cells (NSC) made from either retroviral, episomal, or synthetic mRNA cell reprogramming methods. Global proteomic analysis reveals the main differences in signal transduction and immune cell protein expression between F1 and F2 cells, implicating wild type (WT) toll like receptor protein 3 (TLR3). Furthermore, global methylome analysis identified an isoform of the human TLR3 gene that is not epigenetically reset correctly upon differentiation to F2 cells resulting in a hypomethylated transcription start site in the TLR3 isoform promoter and overexpression in most human iPSC-derived cells not seen in normal human tissue. The human TLR3 isoform in human iPSC-NSC functions to suppress NF-KB p65 signaling pathway in response to virus (Poly IC), suggesting suppressed immunity of iPSC-derived cells to viral infection. The sustained WT TLR3 and TLR3 isoform overexpression is central to understanding the altered immunogenicity of human iPSC-derived cells calling for screening of human iPSC-derived cells for TLR3 expression levels before applications. Stem Cells 2019 :476–488
Publisher: Oxford University Press (OUP)
Date: 16-09-2022
Abstract: Biomedical science is rapidly developing in terms of more transparency, openness and reproducibility of scientific publications. This is even more important for all studies that are based on results from basic semen examination. Recently two concordant documents have been published: the 6th edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen, and the International Standard ISO 23162:2021. With these tools, we propose that authors should be instructed to follow these laboratory methods in order to publish studies in peer-reviewed journals, preferable by using a checklist as suggested in an Appendix to this article.
Location: United States of America
Location: Spain
No related grants have been discovered for Rafael Oliva.