ORCID Profile
0000-0001-5497-4087
Current Organisations
University of the Ryukyus
,
Natural History Museum
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Publisher: Springer Science and Business Media LLC
Date: 10-2023
Publisher: Cold Spring Harbor Laboratory
Date: 30-05-2020
DOI: 10.1101/2020.05.27.116905
Abstract: As global bio ersity declines, there’s an increasing need to create an educated and engaged society. Having people from all ages participate in measuring bio ersity where they live helps to create awareness. Recently, the use of environmental DNA (eDNA) for bio ersity surveys has gained momentum. Here, we test whether s ling eDNA and metabarcoding can be used for rapid urban bio ersity surveys for educational purposes. We s led 2×1 L of water from each of 15 locations in the city of Trondheim, Norway, including a variety of freshwater, marine and brackish habitats. DNA was extracted, lified in triplicate for the COI gene and sequenced. The obtained data were analysed on the novel mBRAVE platform, an online open access software and computing resource. The water s les were collected in two days by two people and the lab analysis was completed in five days by one person. Overall, we detected the presence of 501 taxa identified as belonging to 435 species, representing 90 orders and 18 phyla. On average, only 5.4% of the taxa were shared among six replicates per site. Based on the observed ersity, three distinct clusters were detected and related to geographic distribution of sites. There were some taxa shared between the habitats, with a substantial presence of terrestrial biota. Our results match expected patterns of bio ersity in the landscape and show that with minimal s ling effort, hundreds of species can be detected. Thus, using eDNA analysis of water is promising for rapid bio ersity surveys, and it is likely that more detailed results could be obtained by optimising field and lab methods for particular groups of interest. We recommend that rapid eDNA surveys, with openly available services and softwares, can be used to raise awareness in the importance of bio ersity.
Publisher: Magnolia Press
Date: 28-05-2018
DOI: 10.11646/ZOOTAXA.4424.1.1
Abstract: A molecular phylogeny and a review of family-group classification are presented for 137 species (ca. 125 genera) of the insect family Cicadidae, the true cicadas, plus two species of hairy cicadas (Tettigarctidae) and two outgroup species from Cercopidae. Five genes, two of them mitochondrial, comprise the 4992 base-pair molecular dataset. Maximum-likelihood and Bayesian phylogenetic results are shown, including analyses to address potential base composition bias. Tettigarcta is confirmed as the sister-clade of the Cicadidae and support is found for three subfamilies identified in an earlier morphological cladistic analysis. A set of paraphyletic deep-level clades formed by African genera are together named as Tettigomyiinae n. stat. Taxonomic reassignments of genera and tribes are made where morphological examination confirms incorrect placements suggested by the molecular tree, and 11 new tribes are defined (Arenopsaltriini n. tribe, Durangonini n. tribe, Katoini n. tribe, Lacetasini n. tribe, Macrotristriini n. tribe, Malagasiini n. tribe, Nelcyndanini n. tribe, Pagiphorini n. tribe, Pictilini n. tribe, Psaltodini n. tribe, and Selymbriini n. tribe). Tribe Tacuini n. syn. is synonymized with Cryptotympanini, and Tryellina n. syn. is synonymized with an expanded Tribe Lamotialnini. Tribe Hyantiini n. syn. is synonymized with Fidicinini. Tribe Sinosenini is transferred to Cicadinae from Cicadettinae, Cicadatrini is moved to Cicadettinae from Cicadinae, and Ydiellini and Tettigomyiini are transferred to Tettigomyiinae n. stat from Cicadettinae. While the subfamily Cicadinae, historically defined by the presence of timbal covers, is weakly supported in the molecular tree, high taxonomic rank is not supported for several earlier clades based on unique morphology associated with sound production.
Publisher: Elsevier BV
Date: 04-2015
DOI: 10.1016/J.AJO.2014.12.025
Abstract: To investigate changes in choroidal thickness after aflibercept therapy for neovascular age-related macular degeneration (AMD). Retrospective, consecutive, interventional case series. This study included 102 eyes of 102 patients with treatment-naïve neovascular AMD. All 102 eyes underwent 3 consecutive monthly 2.0 mg intravitreal aflibercept injections at baseline, 1 month, and 2 months. Choroidal thickness during 3 months were evaluated using either swept-source optical coherence tomography (OCT) or enhanced-depth imaging OCT. Of the 102 eyes, 46 eyes (45.1%) were diagnosed as typical neovascular AMD and 56 eyes (54.9%) as polypoidal choroidal vasculopathy. After intravitreal aflibercept injections, the mean subfoveal choroidal thickness decreased from 252.0 ± 99.7 μm at baseline to 217.9 ± 95.6 μm at 3 months (P < .0001 percentage change from baseline, 86.5%). Mean choroidal thickness measured at 3 mm from the foveal center in the superior, inferior, temporal, and nasal directions also decreased significantly from 258.7 ± 85.9 μm to 236.4 ± 84.6 μm, 229.9 ± 93.0 μm to 208.6 ± 86.5 μm, 237.4 ± 86.5 μm to 214.6 ± 79.5 μm, and 183.7 ± 97.0 μm to 162.3 ± 90.6 μm, respectively (P < .0001 for all directions). Both subtypes of neovascular AMD demonstrated a similar trend toward decreasing choroidal thickness during the follow-up period. Choroidal thickness significantly decreased not only at the foveal center but also in the entire macula after 3 monthly intravitreal aflibercept injections for neovascular AMD.
Publisher: American Association for the Advancement of Science (AAAS)
Date: 29-06-2018
Abstract: National laws fearing biopiracy squelch taxonomy studies
Publisher: Wiley
Date: 24-10-2020
DOI: 10.1002/EDN3.152
Publisher: Elsevier BV
Date: 10-2023
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Benjamin Price.