ORCID Profile
0000-0002-5426-5878
Current Organisations
University of Western Australia
,
Institute of Physical Chemistry, Polish Academy of Sciences
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Publisher: SPIE
Date: 10-02-2011
DOI: 10.1117/12.875212
Publisher: SPIE
Date: 26-04-2016
DOI: 10.1117/12.2214712
Publisher: OSA
Date: 2013
Publisher: SPIE
Date: 08-03-2012
DOI: 10.1117/12.908848
Publisher: SPIE
Date: 20-03-2013
DOI: 10.1117/12.2007208
Publisher: AIP Publishing
Date: 06-2021
DOI: 10.1063/5.0052258
Abstract: Many bioimaging studies, including those in engineered tissue constructs, intravital microscopy in animal models, and medical imaging in humans, require cellular-resolution imaging of structures deep within a s le. Yet, many of the current approaches are limited in terms of resolution, but also in invasiveness, repeatable imaging of the same location, and accessible imaging depth. We coin the term micro-endomicroscope to describe the emerging class of small, cellular-resolution endoscopic imaging systems designed to image cells in situ while minimizing perturbation of the s le. In this Perspective, we motivate the need for further development of micro-endomicroscopes, highlighting applications that would greatly benefit, reviewing progress, and considering how photonics might contribute. We identify areas ripe for technological development, such as micro-scanners and small lens systems, that would advance micro-endomicroscope performance. With the right developments in photonics, many possibilities exist for new minimally invasive translatable imaging tools across the scientific, pre-clinical, and clinical spectrum: from longitudinal studies of engineered tissue constructs, to tracking disease progression in animal models, to expanding the ability to diagnose and develop treatments for diseases without the need for invasive medical procedures.
Publisher: Optica Publishing Group
Date: 24-01-2020
DOI: 10.1364/BOE.382755
Abstract: The organization of fibrillar tissue on the micrometer scale carries direct implications for health and disease but remains difficult to assess in vivo . Polarization-sensitive optical coherence tomography measures birefringence, which relates to the microscopic arrangement of fibrillar tissue components. Here, we demonstrate a critical improvement in leveraging this contrast mechanism by employing the improved spatial resolution of focus-extended optical coherence microscopy (1.4 µm axially in air and 1.6 µm laterally, over more than 70 µm depth of field). Vectorial birefringence imaging of sheep cornea ex vivo reveals its lamellar organization into thin sections with distinct local optic axis orientations, paving the way to resolving similar features in vivo .
Publisher: BMJ
Date: 03-2010
Abstract: To demonstrate the applicability of ultrahigh-speed, ultrahigh-resolution spectral optical coherence tomography (SOCT) to cross-sectional imaging of the capsular bag in vivo. The ultrahigh-speed and ultrahigh-resolution SOCT prototype was designed and constructed at Nicolaus Copernicus University (Torun, Poland). To obtain an ultrahigh speed up to 100,000 lines/s a new spectrometer with fast CMOS line-scan camera was built. A femtosecond laser with a central wavelength of 780 nm and Deltalambda=160 nm enabled imaging with an axial resolution of 2.3 microm and lateral resolution of 10 microm in tissue. Lens capsules of two healthy eyes were examined with the aid of the instrument using two- and three-dimensional scanning protocols. The prototype provided ultrahigh-resolution tomograms composed of 8000 A-scans with an acquisition time of 0.16 s. The quality was sufficient to evaluate the capsular bag and to estimate its thickness. It was possible to visualise a separate layer of lens epithelium, to the authors' knowledge the first such visualisation. Three-dimensional data were used to produce lens-capsule thickness maps. Ultrahigh-resolution, ultrahigh-speed SOCT based on a femtosecond laser allows two- and three-dimensional evaluation of a capsular bag and lens epithelium. The instrument provides new information of scientific and clinical value.
Publisher: SPIE
Date: 02-03-2015
DOI: 10.1117/12.2076890
Publisher: SPIE
Date: 24-04-2017
DOI: 10.1117/12.2251519
Publisher: The Optical Society
Date: 15-10-2018
DOI: 10.1364/BOE.9.005437
Publisher: SPIE
Date: 12-02-2009
DOI: 10.1117/12.808555
Publisher: AIP Publishing
Date: 04-2022
DOI: 10.1063/5.0072223
Abstract: Mammographic breast density is a strong breast cancer risk factor, and its routine clinical measurement could potentially be used to identify women at higher risk of breast cancer and/or monitor primary prevention strategies. Previous reports of optical breast spectroscopy (OBS), a novel approach to measuring breast density, demonstrated that it is safe (no ionizing radiation), portable, low-cost, and does not require image interpretation but have been limited to small, single-center studies. Reference measurements taken on a phantom breast prior to and after each woman’s OBS assessment are required for the calibration of the system transfer function as a part of processing participant data. To inform the validity of participant data, a detailed description of the reference measurements and a repeatability analysis of these measurements taken before and after participant assessment is presented. Reference measurements for OBS from 539 women aged 18–40 years were obtained as a part of a high-throughput epidemiological pilot study. Of these, measurements from 20 women with no useable data due to device failure (3.7%) were excluded and from another 12 women due to user error. The intra-class correlation (ICC) within complete pairs of reference data (taken before and after assessment) was high (all ICC & 0.84). The analysis presented here confirms the OBS participant data as valid for use in ongoing epidemiological research, providing further supporting evidence of OBS as a measure of breast density. A novel method of measuring breast density is needed to bridge large gaps in the knowledge of breast density in younger women and its relation to later-life breast cancer risk.
Publisher: The Optical Society
Date: 21-07-2014
DOI: 10.1364/OE.22.018177
Publisher: Optica Publishing Group
Date: 06-08-2009
DOI: 10.1364/OE.17.014880
Abstract: We present an application of in vivo anterior segment imaging of the human eye with an ultrahigh speed swept source OCT instrument. For this purpose, a dedicated OCT system was designed and constructed. This instrument enables axial zooming by automatic reconfiguration of spectral sweep range an enhanced imaging range mode enables imaging of the entire anterior segment while a high axial resolution mode provides detailed morphological information of the chamber angle and the cornea. The speed of 200,000 lines/s enables high s ling density in three-dimensional imaging of the entire cornea in 250 ms promising future applications of OCT for optical corneal topography, pachymetry and elevation maps. The results of a preliminary quantitative corneal analysis based on OCT data free form motion artifacts are presented. Additionally, a volumetric and real time reconstruction of dynamic processes, like pupillary reaction to light stimulus or blink-induced contact lens movements are demonstrated.
Publisher: OSA
Date: 2010
Publisher: Optica Publishing Group
Date: 14-10-2020
DOI: 10.1364/BOE.402402
Abstract: Corneal biomechanics play a fundamental role in the genesis and progression of corneal pathologies, such as keratoconus in corneal remodeling after corneal surgery and in affecting the measurement accuracy of glaucoma biomarkers, such as the intraocular pressure (IOP). Air-puff induced corneal deformation imaging reveals information highlighting normal and pathological corneal response to a non-contact mechanical excitation. However, current commercial systems are limited to monitoring corneal deformation only on one corneal meridian. Here, we present a novel custom-developed swept-source optical coherence tomography (SSOCT) system, coupled with a collinear air-puff excitation, capable of acquiring dynamic corneal deformation on multiple meridians. Backed by numerical simulations of corneal deformations, we propose two different scan patterns, aided by low coil impedance galvanometric scan mirrors that permit an appropriate compromise between temporal and spatial s ling of the corneal deformation profiles. We customized the air-puff module to provide an unobstructed SSOCT field of view and different peak pressures, air-puff durations, and distances to the eye. We acquired multi-meridian corneal deformation profiles (a) in healthy human eyes in vivo , (b) in porcine eyes ex vivo under varying controlled IOP, and (c) in a keratoconus-mimicking porcine eye ex vivo . We detected deformation asymmetries, as predicted by numerical simulations, otherwise missed on a single meridian that will substantially aid in corneal biomechanics diagnostics and pathology screening.
Publisher: The Optical Society
Date: 16-12-2013
DOI: 10.1364/BOE.5.000259
Publisher: The Optical Society
Date: 07-05-2015
DOI: 10.1364/OL.40.002277
Publisher: Wiley
Date: 13-07-2018
Abstract: We employ optical coherence tomography (OCT) and optical coherence microscopy (OCM) to study conjunctival lymphatics in porcine eyes ex vivo. This study is a precursor to the development of in vivo imaging of the collecting lymphatics for potentially guiding and monitoring glaucoma filtration surgery. OCT scans at 1300 nm and higher‐resolution OCM scans at 785 nm reveal the lymphatic vessels via their optical transparency. Equivalent signal characteristics are also observed from blood vessels largely free of blood (and devoid of flow) in the ex vivo conjunctiva. In our lymphangiography, vessel networks were segmented by compensating the depth attenuation in the volumetric OCT/OCM signal, projecting the minimum intensity in two dimensions and thresholding to generate a three‐dimensional vessel volume. Vessel segmentation from multiple locations of a range of porcine eyes ( n = 21) enables visualization of the vessel networks and indicates the varying spatial distribution of patent lymphatics. Such visualization provides a new tool to investigate conjunctival vessels in tissue ex vivo without need for histological tissue processing and a valuable reference on vessel morphology for the in vivo label‐free imaging studies of lymphatics to follow.
Publisher: Photonics Society of Poland
Date: 10-2018
Abstract: The deformation litudes measured with air-puff OCT are sensitive to both (intraocular pressure) IOP and biomechanical properties of the cornea. Analysis of the litudes of corneal deformation is challenging due to interrelation of IOP and corneal biomechanics. In this study, we used natural diurnal IOP fluctuations to investigate corneal deformations in a number of subjects whose eyes were measured multiple times during a day. The results of analysis, based on corneal hysteresis, revealed a corneal hysteresis parameter, which remains constant during a day for each in idual eye. We hypothesize that above-mentioned metric might correlate with biomechanical properties of the cornea without influence of IOP. Full Text: PDF ReferencesMeek KM, Tuft SJ, Huang Y, Gill PS, Hayes S, Newton RH, Bron AJ, "Changes in Collagen Orientation and Distribution in Keratoconus Corneas", Invest Ophthalmol Vis Sci, 2005. 46(6): p. 1948-56. CrossRef Zimmermann DR, Fisher RW, Winterhalter KH, Witmer R, Vaughan L, "Comparative studies of collagens in normal and keratoconus corneas", Exp Eye Res, 1988. 46(3): p. 431-42. CrossRef Andreassen TT, Simonsen AH, and Oxlund H, "Biomechanical properties of keratoconus and normal corneas", Experimental Eye Research, 1980. 31(4): p. 435-441. CrossRef Heijl A, Leske MC, Bengtsson B, Hyman L, Bengtsson B, Hussein M, "Reduction of Intraocular Pressure and Glaucoma Progression", Arch Ophthalmol, 2002. 120(10): p. 1268-79. CrossRef Chauhan BC and Drance SM, "The influence of intraocular pressure on visual field damage in patients with normal-tension and high-tension glaucoma", Investigative Ophthalmology & Visual Science, 1990. 31(11): p. 2367-2372. DirectLink Gelaw Y, "The impact of central corneal thickness on intraocular pressure among Ethiopian glaucoma patients: a cross-sectional study", BMC Ophthalmology, 2012. 12(1): p. 58. CrossRef Doughty MJ and Zaman ML, "Human Corneal Thickness and Its Impact on Intraocular Pressure Measures: A Review and Meta-analysis Approach", Surv Ophthalmol, 2000. 44(5): p. 367-408. CrossRef Liu J, and Roberts CJ, "Influence of corneal biomechanical properties on intraocular pressure measurement: Quantitative analysis", J Cataract Refract Surg, 2005. 31(1): p. 146-55. CrossRef Ehlers N, Hansen FK, and Aasved H, "Biometric Correlations of Corneal Thickness", Acta Ophthalmol (Copenh), 1975. 53(4): p. 652-9. CrossRef Harada Y, Hirose N, Tawara A, "The Influence of Central Corneal Thickness and Corneal Curvature Radius on The Intraocular Pressure as Measured By Different Tonometers: Noncontact and Goldmann Applanation Tonometers", J Glaucoma, 2008. 17(8): p. 619-25. CrossRef Alonso-Caneiro D, Karnowski K, Kaluzny BJ, Kowalczyk A, Wojtkowski M, "Assessment of corneal dynamics with high-speed swept source Optical Coherence Tomography combined with an air puff system", Optics Express, 2011. 19(15): p. 14188-14199. CrossRef Dorronsoro C, Pascual D, Perez-Merino P, Kling S and Marcos S, "Dynamic OCT measurement of corneal deformation by an air puff in normal and cross-linked corneas", Biomedical Optics Express, 2012. 3(3): p. 473-487. CrossRef Karnowski K, Kaluzny BJ, Szkulmowski M, Gora M, Wojtkowski M, "Corneal topography with high-speed swept source OCT in clinical examination", Biomedical Optics Express, 2011. 2(9): p. 2709-2720. CrossRef A. N. S. Institute, "American National Standard for Safe use of Lasers," (American National Standards Institute, Orlando, FL, 2000) DirectLink David R, Zangwill L, Briscoe D, Dagan M, Yagev R, Yassur Y, "Diurnal intraocular pressure variations: an analysis of 690 diurnal curves", Br J Ophthamlom, 1992, 76(5): p. 280-282 CrossRef Maczynska E, Karnowski K, Szulzycki K, Malinowska M, Dolezyczek H, Cichanski A, Wojtkowski M, Kaluzny BJ, Grulkowski I, Journal of Biophotonics (to be published).
Publisher: Wiley
Date: 04-11-2015
DOI: 10.1111/CTS.12344
Publisher: SPIE
Date: 04-03-2016
DOI: 10.1117/12.2209615
Publisher: The Optical Society
Date: 22-03-2019
Publisher: Public Library of Science (PLoS)
Date: 26-01-2015
Publisher: The Optical Society
Date: 29-08-2011
DOI: 10.1364/BOE.2.002709
Publisher: Optica Publishing Group
Date: 12-08-2020
DOI: 10.1364/BOE.400723
Abstract: We present in-vivo imaging of the mouse brain using custom made Gaussian beam optical coherence microscopy (OCM) with 800nm wavelength. We applied new instrumentation to longitudinal imaging of the glioblastoma (GBM) tumor microvasculature in the mouse brain. We have introduced new morphometric biomarkers that enable quantitative analysis of the development of GBM. We confirmed quantitatively an intensive angiogenesis in the tumor area between 3 and 14 days after GBM cells injection confirmed by considerably increased of morphometric parameters. Moreover, the OCM setup revealed heterogeneity and abnormality of newly formed vessels.
Publisher: The Optical Society
Date: 18-01-2018
Publisher: Wiley
Date: 14-10-2018
Publisher: SPIE
Date: 19-04-2017
DOI: 10.1117/12.2254815
Publisher: The Optical Society
Date: 06-08-2014
DOI: 10.1364/OL.39.004727
Publisher: Springer Science and Business Media LLC
Date: 23-06-2017
DOI: 10.1038/S41598-017-04220-8
Abstract: Imaging of living cells based on traditional fluorescence and confocal laser scanning microscopy has delivered an enormous amount of information critical for understanding biological processes in single cells. However, the requirement for a high numerical aperture and fluorescent markers still limits researchers’ ability to visualize the cellular architecture without causing short- and long-term photodamage. Optical coherence microscopy (OCM) is a promising alternative that circumvents the technical limitations of fluorescence imaging techniques and provides unique access to fundamental aspects of early embryonic development, without the requirement for s le pre-processing or labeling. In the present paper, we utilized the internal motion of cytoplasm, as well as custom scanning and signal processing protocols, to effectively reduce the speckle noise typical for standard OCM and enable high-resolution intracellular time-lapse imaging. To test our imaging system we used mouse and pig oocytes and embryos and visualized them through fertilization and the first embryonic ision, as well as at selected stages of oogenesis and preimplantation development. Because all morphological and morphokinetic properties recorded by OCM are believed to be biomarkers of oocyte/embryo quality, OCM may represent a new chapter in imaging-based preimplantation embryo diagnostics.
Publisher: Institute of Electrical and Electronics Engineers (IEEE)
Date: 10-2022
Publisher: OSA
Date: 2017
Publisher: The Optical Society
Date: 20-10-2017
DOI: 10.1364/BOE.8.005127
Publisher: SPIE
Date: 09-02-2012
DOI: 10.1117/12.911124
Publisher: The Optical Society
Date: 11-07-2011
DOI: 10.1364/OE.19.014188
Publisher: SPIE
Date: 28-08-2016
DOI: 10.1117/12.2236988
Publisher: OSA
Date: 2015
Publisher: OSA
Date: 2014
Publisher: Wiley
Date: 23-06-2020
Publisher: IEEE
Date: 10-2015
Location: Poland
No related grants have been discovered for Karol Karnowski.