ORCID Profile
0000-0003-1759-934X
Current Organisation
Hong Kong University of Science and Technology
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Publisher: Wiley
Date: 21-05-2022
Abstract: Liquid‐liquid phase separation (LLPS) drives membraneless organelles (MLOs) formation for organizing biomolecules. Artificial MLOs (AMLOs) have been constructed mostly via the LLPS of engineered proteins capable of regulating limited types of biomolecules. Here, leveraging a minimalist AMLO, driven by LLPS of polymer‐oligopeptide hybrids, enrichment, recruitment, and release of multifaceted cargoes are quantitatively shown, including small fluorescent molecules, fluorophore‐containing macromolecules, proteins, DNAs, and RNAs. Cargoes show up to 10 5 ‐fold enrichment, whilst recruitment and release are triggered by variations of temperature, pH, and/or ionic strength. Also, the first efficacious, rapid, and reversible control of aggregation‐induced emission with over 30 folds of modulation of overall fluorescence intensity is achieved, by intensifying the aggregation of luminogens in AMLO. The AMLO is a simple yet versatile platform for potential drug delivery and biosensor applications.
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2BM00705C
Abstract: To overcome the endosomal barrier, we attached a fusogenic peptide (L17E) onto peptide self-assembled disks, which mimicked the functional subunits of the virus capsid and improved transfection efficiency.
Publisher: Wiley
Date: 06-10-2021
Abstract: Aromatic residues are widely used as building blocks for driving self‐assemblies in natural and designer biomaterials. The noncovalent interactions involving aromatic rings determine proteins’ structure and biofunction. Here, we studied the effects of changes in the proximity of the aromatic rings in a self‐assembling peptide for modulating interactions involving the aromatic residues. By changing the distance between the aromatic ring and peptide backbone and replacing the side chain with a sulfur atom, we altered the nanostructures and gene transfection efficiency of peptide‐DNA co‐assemblies. This study demonstrates the significance of subtle alterations in aromatic interactions and facilitates deeper understanding of the aromatic‐involving interactions.
Publisher: American Chemical Society (ACS)
Date: 21-06-2022
Abstract: Broadening the applicable tools for mRNA delivery provides more flexibility in research and those proven effective and safe can potentially be translated for clinical use. We report here a 27-amino acid peptide sequence mimicking the viral capsid protein, termed pepMAX, capable of co-assembling with mRNA into 100-150 nm nanostructures for efficient transfection of multiple cell lines. The mRNA loading and N/P ratio have been systematically optimized for each cell line. In HeLa, HEK293, and SKNMC, the transfection attained (>80%) is comparable with that of commercially available vectors Lipofectamine MessengerMAX
Publisher: Wiley
Date: 28-09-2023
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2MH00537A
Abstract: Leveraging complex coacervation of a polycation and a bivalent anion with aggregation-induced emission characteristics, we accomplish eight basic logic operations, producing Boolean-like ‘outputs’ with contrast higher than one order of magnitude.
Publisher: Elsevier BV
Date: 06-2022
Publisher: American Chemical Society (ACS)
Date: 17-02-2023
No related grants have been discovered for Ying Chau.