ORCID Profile
0000-0002-6402-7240
Current Organisation
IT University of Copenhagen
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Publisher: Cambridge University Press (CUP)
Date: 25-03-2010
DOI: 10.1017/S0007114510000802
Abstract: Malnutrition during foetal life can induce modifications in the phenotype of an in idual. The present study aimed to observe effects of low foetal life protein provision on modifications of the phenotype and changes in the progeny of 1-year-old female mink (F 1 generation) offspring of mothers fed a low-protein diet. Traits studied included reproductive performance, energy and protein metabolism, and key hepatic enzymes associated with glucose homeostasis and metabolic hormones. The F 0 generation offspring were fed either a low-protein (14 % of metabolisable energy (ME) from protein – FLP1) or an adequate-protein (29 % of ME from protein – FAP1) diet for the last 17·9 ( sd 3·6) d of gestation. The F 1 dams were studied at birth and at 1 year of age, during their first reproductive cycle, after maintenance on an adequate diet from birth and thereafter. Metabolic traits during gestation and lactation were largely unaffected by foetal life protein provision, but birth weight in the F 2 generation was higher ( P = 0·003) among FLP2 kits than among FAP2 kits. Furthermore, the relative abundance of pyruvate kinase mRNA was significantly ( P = 0·007) lower, and fructose-1,6-bisphosphatase mRNA tended ( P = 0·08) to be lower in FLP2 foetuses than in FAP2 foetuses, showing some similar difference in the F 2 generation and F 1 generation foetuses, suggesting an effect on some hepatic enzymes affecting glucose homeostasis being transmitted from the F 1 to the F 2 generation. These findings indicate that even though energy and nitrogen metabolism displayed no effect of protein provision during early life, programming effects still appeared at the molecular level in the following generation.
Publisher: Elsevier BV
Date: 02-2008
Publisher: Informa UK Limited
Date: 02-2010
DOI: 10.1080/17450390903299141
Abstract: Protein malnutrition in utero that induces permanent changes in metabolism has been investigated intensively in various animals in recent years, but to the best of our knowledge, not yet in the mink, a strict carnivore. In the present study, minks were fed either a low-protein (LP) diet, i.e., with a protein:fat:carbohydrate ratio of 14:51:35% of metabolisable energy (ME), or an adequate-protein diet (AP), i.e. 29:56:15% of ME, from when implantation was completed until parturition (17.9 +/- 3.6 days). Respiration and balance experiments were performed during both gestation and lactation. Plasma concentrations of leptin, IGF-1, and insulin were determined by radioimmunoassay the relative abundances of glucose-6-phosphatase (G-6-Pase), fructose-1,6-bisphosphatase (Fru-1,6-P2ase), phosphoenol-pyruvate carboxykinase (PEPCK), and pyruvate kinase (PKM2) were determined in liver, and abundances of adiponectin and leptin in adipose tissue were determined by real-time quantitative PCR (q PCR). The protein supply only affected quantitative metabolism traits during the period of differentiated feeding. The dietary composition was reflected in the nitrogen metabolism and substrate oxidation, but no effects remained during lactation. The LP dams tended to have a smaller liver mass in relation to body weight than did AP dams (2.5% vs. 2.9% p = 0.09), significantly less leptin mRNA (p < 0.05), and 30.6% fewer kits per mated female (p = 0.03). Furthermore, F1-generation kits exposed to protein restriction during foetal life (FLP1 10.3 g) had a lower birth weight (p = 0.004) than did F1-generation kits exposed to adequate protein (FAP1 11.3 g). Differences remained significant until 21 days of age (120.4 g vs. 127.6 g p = 0.005). The FLP1 foetuses displayed a lower abundance of Fru-1,6-P2ase mRNA (p = 0.007) and of PKM2 mRNA (p = 0.002) than did FAP1 foetuses. Whether these changes during foetal life cause permanent changes in the glucose homeostasis of the offspring and result in the transmission of epigenetic phenotypic changes, as seen in the rat, needs further investigation.
Publisher: Elsevier BV
Date: 2012
Publisher: CSIRO Publishing
Date: 2010
DOI: 10.1071/AN09142
Abstract: In this study we test the hypothesis that selecting sheep for a low behavioural reactivity to stressful situations will improve their metabolic efficiency, and thereby feed efficiency, during a controlled trial in an animal house. Twenty-four Merino wethers were used, 12 each from lines selected for high (HBR) and low (LBR) behavioural reactivity to stressful stimuli (human presence and social isolation). The sheep were habituated to the experimental procedures for 10 days, followed by 45 days during which voluntary feed intake was measured so that total daily energy intake was quantified. The sheep were weighed twice weekly before daily feeding. Feed efficiency was determined by measuring net feed intake, average daily weight gain and body condition score. Our hypothesis was not supported by the results of this study. There was no difference between LBR and HBR sheep in average daily weight gain or body condition score. The net feed intake of HBR sheep was lower than that of LBR sheep (P = 0.02), indicating that under the conditions of our experiment, HBR sheep were actually more feed efficient than LBR sheep. This study was carried out on sheep with steady intakes and in familiar surroundings. It is possible that LBR sheep may be more efficient than HBR sheep in more stressful situations.
Publisher: Wiley
Date: 03-08-2013
DOI: 10.1111/JPN.12108
Abstract: Low protein provision in utero and post-partum may induce metabolic disorders in adulthood. Studies in mink have mainly focused on short-term consequences of low protein provision in utero whereas the long-term responses to low protein (LP) provision in metabolically programmed mink are unknown. We investigated whether low protein provision in utero affects the long-term response to adequate (AP) or LP provision after weaning in male mink. Eighty-six male mink were exposed to low (19% of ME from CP crude protein) or adequate (31% of ME from CP) protein provision in utero, and to LP (~20% of ME from CP) or AP (30-42% of ME from CP) provision post-weaning. Being metabolically programmed by low protein provision in utero did not affect the response to post-weaning diets. Dietary protein content in the LP feed after weaning was below requirements evidenced by lower nitrogen retention (p < 0.001) preventing LP mink from attaining their growth potential (p < 0.02). LP mink had a lower liver, pancreas and kidney weight (p < 0.05) as well as lower plasma IGF-1 concentrations at 8 and 25 (p < 0.05) weeks, and a higher incidence of hepatic lipidosis at 25 weeks (p < 0.05). Furthermore, LP mink had a higher body fat (p < 0.05) and lower body CP content (p < 0.05) at 50 weeks of age. It is concluded that some effects of low protein provision in utero can be alleviated by an adequate nutrient supply post-partum. However, long-term exposure to low protein provision in mink reduces their growth potential and induces transient hepatic lipidosis and modified body composition.
Publisher: Elsevier BV
Date: 2008
Publisher: Elsevier BV
Date: 04-2016
Publisher: Elsevier BV
Date: 2016
No related grants have been discovered for Anne-Helene Tauson.