Josef Pfeuffer

Cell volume and water exchange in neural cells monitored by diffusion weighted 1H NMR spectroscopy

Publisher: Elsevier

Date: 2000

DOI: 10.1016/S0079-6123(00)25015-2

Expression of aquaporins in Xenopus laevis oocytes and glial cells as detected by diffusion-weighted 1H NMR spectroscopy and photometric swelling assay

Publisher: Elsevier BV

Date: 11-1998

DOI: 10.1016/S0167-4889(98)00111-6

Abstract: Expression of aquaporins (AQP) and water permeability were studied in Xenopus laevis oocytes and immobilized glial cells by a pulsed-field gradient spin echo NMR technique and a photometric swelling assay. Oocytes injected with poly(A) RNA from C6-BU-1 cells showed increased swelling behavior under hypoosmotic stress due to expressed water channels as compared to control oocytes. The swelling could be reversibly inhibited by HgCl2. Furthermore, the intracellular relaxation time and the apparent intracellular diffusion coefficient of water in oocytes were determined by diffusion-weighted 1H NMR experiments to be T2=36 ms and Dapp, intra=0.18x10-3 mm2/s. In immobilized C6 and F98 cells the mean exchange time of intracellular water was found to be 51 ms which increased to 75 ms upon chronic treatment (4 days) in hypertonic medium. Additional hybrid depletion experiments with antisense oligonucleotides directed against AQP1 were performed on oocytes and C6 cells. Moreover, different water channel subtypes of glial cells were assessed by a reverse transcriptase polymerase chain reaction assay. With this, the mRNA encoding AQP1 could be detected in primary cultures and glial cell lines, whereas AQP4 mRNA was found in astroglia-rich primary cultures, but not in F98 and C6 cells. Our results show that water permeability in glial cells is mainly mediated by water channels which play an important role in the regulation of water flow in brain under normal and pathological conditions.

Pulsed arterial spin labelling at ultra-high field with a B 1 + -optimised adiabatic labelling pulse

Publisher: Springer Science and Business Media LLC

Date: 15-04-2016

DOI: 10.1007/S10334-016-0555-2

Abstract: Arterial spin labelling (ASL) techniques benefit from the increased signal-to-noise ratio and the longer T 1 relaxation times available at ultra-high field. Previous pulsed ASL studies at 7 T concentrated on the superior regions of the brain because of the larger transmit radiofrequency inhomogeneity experienced at ultra-high field that hinders an adequate inversion of the blood bolus when labelling in the neck. Recently, researchers have proposed to overcome this problem with either the use of dielectric pads, through dedicated transmit labelling coils, or special adiabatic inversion pulses. We investigate the performance of an optimised time-res led frequency-offset corrected inversion (TR-FOCI) pulse designed to cause inversion at much lower peak B 1 (+) . In combination with a PICORE labelling, the perfusion signal obtained with this pulse is compared against that obtained with a FOCI pulse, with and without dielectric pads. Mean grey matter perfusion with the TR-FOCI was 52.5 ± 10.3 mL/100 g/min, being significantly higher than the 34.6 ± 2.6 mL/100 g/min obtained with the FOCI pulse. No significant effect of the dielectric pads was observed. The usage of the B 1 (+) -optimised TR-FOCI pulse results in a significantly higher perfusion signal. PICORE-ASL is feasible at ultra-high field with no changes to operating conditions.

Siemens Healthcare

Location: Germany

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Linkage Projects - Grant ID: LP200301393

Start Date: 02-2022

End Date: 02-2025

Amount: $380,115.00

Funder: Australian Research Council