ORCID Profile
0000-0002-9117-6911
Current Organisations
Universitas SembilanBelas November Kolaka
,
Cardiff University
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Publisher: Frontiers Media SA
Date: 17-03-2020
Publisher: Negah Scientific Publisher
Date: 30-10-2019
DOI: 10.32598/JCCNC.5.1.1
Publisher: Elsevier BV
Date: 09-2015
DOI: 10.1016/J.IJANTIMICAG.2015.04.015
Abstract: There is a strong association between vaginal and/or amniotic fluid Ureaplasma spp. colonisation and risk of preterm birth. The novel fluoroketolide antibiotic solithromycin (CEM-101) is active against Ureaplasma spp. in vitro. Evidence from ex vivo and in vivo models suggests that, unlike most macrolide antibiotics, solithromycin readily crosses the placenta. Solithromycin metabolism varies according to species in pregnant sheep, the bioactive metabolites CEM-214 and N-acetyl-CEM-101 (NAc-CEM-101) have been shown to accumulate in the amniotic cavity following maternal solithromycin administration, potentially contributing to its antimicrobial effects. To determine the antimicrobial activity of these metabolites against Ureaplasma spp., the effects of solithromycin, CEM-214, NAc-CEM-101 and the comparator azithromycin were tested on a collection of 100 clinical Ureaplasma spp. isolates from the UK and Australia using a modified 96-well broth microdilution method. MIC90 values observed for the combined cohort were: solithromycin, 0.125 mg/L CEM-214, 0.5mg/L NAc-CEM-101, 0.5mg/L and azithromycin, 2mg/L. Solithromycin showed 34-fold greater activity against Ureaplasma spp. isolates than azithromycin, whilst CEM-214 and NAc-CEM-101 possessed ca. 22% and 17% of the activity of solithromycin, respectively, significantly greater than that of azithromycin. One bacterial isolate showed resistance to azithromycin (MIC=16 mg/L) but had a much lower MIC for solithromycin (MIC=0.25mg/L). In conclusion, the metabolites of solithromycin had reduced, but still potent, activity against 100 clinical Ureaplasma spp. isolates in vitro. This may be important in some instances such as pregnancy, however studies to determine levels of the metabolites in these settings are required.
Publisher: Springer Science and Business Media LLC
Date: 2017
Abstract: Several recent studies have identified a potential role for intrauterine Candida albicans in adverse pregnancy outcomes, including preterm birth. There is, however, a limited understanding of the impact of intrauterine candida infection on fetal well-being in early pregnancy. Using a sheep model of early pregnancy, the aims of this study were to determine (1) the ability of experimentally induced intrauterine C albicans to infect the fetus and (2) whether C albicans exposure in early pregnancy is associated with alterations in fetal cardiac function, as measured by spectral tissue Doppler imaging analysis of fetal cardiac function. Merino ewes carrying singleton pregnancies at 89 days' gestation (term is ∼150 days) received C albicans (n = 8) via ultrasound-guided intra-amniotic injection. Saline-exposed fetuses served as controls (n = 6). Spectral tissue Doppler imaging echocardiography and amniotic fluid collection were performed at baseline and 24 and 72 hours after intrauterine C albicans injection. Fetal tissues were collected at postmortem for analysis of infection and inflammation. Relative to saline control, intrauterine C albicans infection resulted in pronounced increases in amniotic fluid tumor necrosis factor α (TNF-α P < .05) and cytokine/chemokine messenger RNA (interleukin [IL] 1β, IL-6, TNF-α, and monocyte chemoattractant protein 1 P < .05) in the fetal myocardium, lung, skin, and liver at 72 and 96 hours postinfection. Spectral tissue Doppler imaging showed diastolic dysfunction at 24 hours and severe biventricular diastolic dysfunction 72 hours postinfection. Intrauterine C albicans infection in a sheep model of early pregnancy causes systemic fetal candidiasis, which is associated with a robust systemic inflammatory response and progressive cardiac dysfunction detectable by spectral tissue Doppler imaging.
Publisher: Poltekkes Kemenkes Surakarta
Date: 23-09-2021
Abstract: Background: The prevalence of chronic disease in elderly increase year by year worldwide and it’s related to mortality and decrease of quality of life. The majority of elderly patients complaints about sleep disorders and that affects sleep quality, health status and quality of life. Psychological aspect was identified as one main contributor in sleep disorders among elderly who suffer from chronic disease. This study aims to determine the effect of psychological distress on sleep quality among elderly patients with chronic disease. Methods: This study applied a quantitative research design with cross-sectional approach. The study conducted in a teaching university hospital from October to November 2021. Population is elderly with chronic diseases, the participants selected by using total s ling method. Data collection using psychological distress related questionnaire and Pittsburg Sleep Quality Index (PSQI). Data analysis applied Fishers’ Exact test. Results: There are 52 older patients participate in this study. The study finding 85.6% participants have psychological distress and 51.9% have poor quality of sleep. Based on Fishers’ Exact test analysis, the p-value was 0.308 (statistically insignificant). Conclusion: This study finding report that psychological distress statistically has no significant affect on sleep quality among elder patients who suffer from chronic disease. However, some participants have poor sleep quality. Study in advance to investigate how psychological status affect sleep quality is needed.
Publisher: Institute of Advanced Engineering and Science
Date: 06-2021
DOI: 10.11591/IJPHS.V10I2.20722
Abstract: Coronavirus disease (COVID-19) infection has been a major public health-related issue worldwide, including Indonesia. The COVID-19 cases still escalating until this study finished in June 2020 due to the disease positively spreading from person to person. To reduce the spreading of the disease, investigating Indonesian’s knowledge, attitudes, and practice on COVID prevention and mitigation during the outbreak period was crucial. A survey cross-sectional was conducted and using social networking apps to recruit participants. All Indonesian residents who have 18 years old and over and living in Indonesia during the COVID-19 outbreak were voluntarily invited to participate in this study. There are 3464 participants involved in this study. The mean age was 27.63 years, and mostly were women, domicile in Java, held bachelor degrees, students, Muslims, and single/never married. The lowest mean practices score was found among residents who live in Maluku, Papua, and Papua Barat, senior high qualification, unemployed, indigenous religions, and single/never married. The majority of Indonesian residents have good knowledge, positive attitudes, and good practice to promote COVID-19 prevention and mitigation to reduce the pandemic spreading within the country. However, there is a lack of knowledge, attitudes, and practice among a particular group of participants.
Publisher: LP3M Universitas Sains Al Quran
Date: 30-09-2019
Abstract: Kanker termasuk salah satu penyakit yang berkontribusi terhadap kematian di seluruh dunia. Kanker payudara adalah jenis kanker yang sering diterjadi pada wanita, terutama pada wanita yang usianya menjelang 50 tahun. Di Indonesia, angka kejadian kanker semakin meningkat dari tahun ke tahun, dimana salah satu pemicu adalah adanya perubahan gaya hidup. Upaya yang dapat dilakukan untuk menurunkan insiden kanker payudara stadium lanjut yaitu dengan melakukan pemeriksaan payudara secara rutin. Dengan pemeriksaan tersebut yang dilakukan teratur maka penemuan atau skrining dan pendeteksian secara dini dapat mengurangi angka kejadian kanker payudara stadium lanjut. Kegiatan ini dilakukan berupa penyuluhan dengan metode ceramah tanya jawab serta demonstrasi mengenai Breast Self Examination (BSE). Tujuan kegiatan ini diharapkan dapat meningkat pengetahuan dan skill para kader kesehatan sehingga mereka dapat menjadi tenaga yang mahir dalam melakukan pemeriksaan BSE tersebut. Lebih lanjut, kader juga diharapkan dapat mengajarkan kepada para wanita usia subur lainnnya di wilayah tempat tingggalnya. Setelah kegiatan ini laksanakan, pengetahuan dan keter ilan kader kesehatan terkait pencegahan dan deteksi dini kanker payudara dan pemeriksaan payudara sendiri mengalami peningkatan. Kesimpulan pelatihan dapat meningkatkan pengetahuan dan keter ilan kader kesehatan terkait pencegahan dan deteksi dini kanker payudara dan pemeriksaan payudara sendiri secara mandiri.
Publisher: Center for Journal Management and Publication, Lambung Mangkurat University
Date: 08-2020
Publisher: Universitas Muhammadiyah Magelang
Date: 31-01-2021
DOI: 10.31603/NURSING.V8I1.3942
Abstract: Currently, cancer includes breast cancer is a predominant disease treated in palliative care services. Breast cancer incidence increased significantly during the last decade and can progress to a late or advanced stage. At this advanced stage, the incidence of the fungating wound occurs in 5-10% of breast cancer patients. The study aims to identify assessment and symptoms management of fungating breast cancer in the palliative care setting. This study applied a literature review method. Searching for articles using 4 journal databases including DOAJ, Google Scholar, Proquest, and Science Direct. 17 articles that met the inclusion criteria of the study. The literature review identifies that the assessment tools are holistic assessment methods, wound assessment charts, and time framework assessments. The major symptoms are mal-odor, pain, exudation, peri-wound maceration and bleeding, psychological and spiritual issues. The studies, which focus on assessment and symptoms management of fungating breast cancer was limited. This affects the comprehensiveness of the review study. Investigation on quality of life among fungating breast cancer patients shortly is needed.
Publisher: Lembaga Layanan Pendidikan Tinggi - LLDIKTI - Wilayah X
Date: 15-07-2022
Abstract: Stunting merupakan masalah kurangnya asupan gizi dalam waktu yang cukup lama, sehingga mengakibatkan gangguan pertumbuhan karena kurang gizi kronis sehingga menyebabkan tinggi badan anak lebih rendah atau pendek (Kerdil) dari standar usianya. Secara teori banyak faktor yang dapat menyebabkan anak menderita stunting. Sebagai proiritas nasional bangsa Indonesia, upaya pencegahan dan penanggulangan stunting harus dihadapi secara bersama oleh seluruh elemen bangsa. Penelitian ini bertujuan untuk menganalisis determinan kejadian stunting di Kabupaten Kolaka. Penelitian ini menggunakan pendekatan studi kualitatif dengan metode wawancara secara mendalam dimana, data dikumpulkan dari proses wawancara semi-terstruktur, observasi, analisis teks/ studi dokumen dan menggunakan rekaman audio dan vidio. Hasil penelitian menunjukkan kenaikan berat badan pada saat hamil tidak optimal, pengetahuan yang dimiliki orang tua juga kurang, orang tua masih merokok, pemberian makanan tambahan yang tidak tepat sasaran, makanan instan yang tidak sesuai kebutuhan anak masih diberikan serta informasi kesehatan yang sangar kurang didapatkan. Adapun kesimpulan dalam penelitian ini yaitu kurangnya pengetahuan yang dimiliki orang tua sehingga memberikan d ak terhadap perilaku dalam mengasuh anaknya tidak memperhatikan tumbuh kembangnya. Tidak khawatir Ketika pertumbuhan dan perkembangan anaknya berada dibawah standar. Salah satu penyebabnya yaitu informasi Kesehatan mengenai stunting mulai dari penyebab hingga akibat yang didapatkan itu kurang Kata kunci : Stunting prengetahuan merokok sumber makanan informasi kesehatan
Publisher: Lembaga Layanan Pendidikan Tinggi - LLDIKTI - Wilayah X
Date: 20-02-2022
Publisher: Universitas Muhammadiyah Bengkulu
Date: 27-04-2022
Abstract: Comparison of EORTC QLQ-C30 and WHOQOL-BREF Measurement Results to Measure Quality of Life in CancerPatients: A Literature Review. It is important for nurses to carry out assessments and evaluate the condition of cancer patients, one of them by measuring their quality of life. The aim of this literature review is to identify the results of measurements of quality of life in cancer patients using EORTC QLQ-C30 and WHOQOL-BREF. Method: Using the PRISMA principle by searching journals through databases from ProQuest, CINAHL, PubMed, and Google Scholar conducted from June - July 2020. Article search is limited from 2010-2020. Assessment of journal article quality using the Joanna Briggs Institute (JBI) checklist. Results: Of 952 journals found, there are 14 journals that fit the research criteria. Quality of life scores measured by EORTC QLQ-C30 are higher than those measured by WHOQOL-BREF. EORTC QLQ-C30 and WHOQOL-BREF have different measurement aspects. Researchers recommend using the EORTC QLQ-C30 to measure the quality of life in cancer patients.
Publisher: STIKES Aisyah Pringsewu Lampung
Date: 09-12-2020
Publisher: Universitas Gadjah Mada
Date: 03-2021
Abstract: Latar Belakang: Hipertensi sebagai penyakit tidak menular saat ini sangat meningkat dan merupakan penyakit pembuluh darah yang dapat menyebabkan terjadinya kematian mendadak sehingga penyakit ini dikenal sebagai silent killer. Meningkatnya persentase ketidakpatuhan meminum obat hipertensi disebabkan berbagai alasan dan hal ini membuat banyaknya pengobatan non-farmakologi yang bersifat alternatif dan komplementer yang bermunculan, salah satunya adalah terapi bekam.Tujuan: Untuk mengetahui pengaruh terapi bekam basah pada penderita hipertensi dalam menurunkan tekanan darah.Metode: Desain penelitian yang digunakan dalam penelitian ini adalah quasy eksperimen dengan pendekatan Control Group Design pre-post test. Populasi dalam penelitian ini adalah penderita hipertensi yang berada di wilayah kerja Puskesmas Kolaka yang memenuhi kriteria inklusi. Teknik pengambilan s el dengan purposive s ling dengan jumlah s el sebanyak 40 responden. Instrumen dilakukan dengan pengukuran tekanan darah dengan menggunakan alat pengukuran tekanan darah dan data dianalisis dengan menggunakan uji friedman test.Hasil: Setelah dilakukan pembekaman basah selama tiga bulan berturut-turut, tekanan darah sistole dan diastole mengalami penurunan secara signifikan pada kelompok intervensi sebesar 0,000 (p 0,05) dan kelompok kontrol (p ,05) sehingga disimpulkan bahwa ada perbedaan rata-rata tekanan darah pada ketiga interval waktu pengukuran pada kelompok intervensi.Kesimpulan: Terapi bekam memiliki pengaruh dalam menurunkan tekanan darah pada penderita hipertensi.
Publisher: American Society for Microbiology
Date: 09-2014
DOI: 10.1128/AAC.03187-14
Abstract: Intrauterine infection with Ureaplasma spp. is strongly associated with preterm birth and adverse neonatal outcomes. We assessed whether combined intraamniotic (IA) and maternal intravenous (IV) treatment with one of two candidate antibiotics, azithromycin (AZ) or solithromycin (SOLI), would eradicate intrauterine Ureaplasma parvum infection in a sheep model of pregnancy. Sheep with singleton pregnancies received an IA injection of U. parvum serovar 3 at 85 days of gestational age (GA). At 120 days of GA, animals ( n = 5 to 8/group) received one of the following treatments: (i) maternal IV SOLI with a single IA injection of vehicle (IV SOLI only) (ii) maternal IV SOLI with a single IA injection of SOLI (IV+IA SOLI) (iii) maternal IV AZ and a single IA injection of vehicle (IV AZ only) (iv) maternal IV AZ and a single IA injection of AZ (IV+IA AZ) or (v) maternal IV and single IA injection of vehicle (control). Lambs were surgically delivered at 125 days of GA. Treatment efficacies were assessed by U. parvum culture, quantitative PCR, enzyme-linked immunosorbent assay, and histopathology. Amniotic fluid (AF) from all control animals contained culturable U. parvum . AF, lung, and chorioamnion from all AZ- or SOLI-treated animals (IV only or IV plus IA) were negative for culturable U. parvum . Relative to the results for the control, the levels of expression of interleukin 1β (IL-1β), IL-6, IL-8, and monocyte chemoattractant protein 2 (MCP-2) in fetal skin were significantly decreased in the IV SOLI-only group, the MCP-1 protein concentration in the amniotic fluid was significantly increased in the IV+IA SOLI group, and there was no significant difference in the histological inflammation scoring of lung or chorioamnion among the five groups. In the present study, treatment with either AZ or SOLI (IV only or IV+IA) effectively eradicated macrolide-sensitive U. parvum from the AF. There was no discernible difference in antibiotic therapy efficacy between IV-only and IV+IA treatment regimens relative to the results for the control.
Publisher: Sekolah Tinggi Ilmu Kesehatan Baiturrahim Jambi
Date: 16-06-2023
Abstract: As an archipelago country, Indonesia has almost 70% of its territory consisting of the sea, and most of the population has a livelihood as fishermen. Traditional fishermen generally are at risk of experiencing barotrauma and decompression due to the low of knowledge and lack of e facilities. This activity aims to improve fisherman ers’ knowledge on barotrauma dna decompression management. Educational methods applied such as teaching, roleplay and discussion. There are 50 traditional fishermen who voluntarily participated, 43 participants (86.0%) have good knowledge about barotrauma and decompression prevention and management, 20 participants (40%) can demonstrate Basic Life Support by guidance, and 12 participants (24%) were able to demonstrate independently. It is hoped that from this activity the fisherman ers community more familiar and understand about barotrauma and decompression as risks from traditionally ing activities.
Publisher: Lembaga Layanan Pendidikan Tinggi - LLDIKTI - Wilayah X
Date: 06-12-2022
Abstract: Most health services recognize the enormous benefits of implementing patient spiritual care in health services. This is realized because humans need not only physically healthy, but also socially, psychologically and spiritually must be maintained from bad influences around them. The purpose of this study was to explore the barriers to the application of spiritual care in health services through a socioecological model perspective. This study used an interview method with a total s le of 18 people. Barriers to the application of spiritual care services from a socioecological perspective, at the level of patient/intrapersonal, family/interpersonal, nurses/service providers, and nursing managers/service providers. Some of the obstacles found were the inability to worship, limited religious facilities, limited knowledge and skills of spiritual care and inadequate human resources. Conclusion This study highlights the need to overcome barriers to spiritual care services at various socio-ecological levels in an effort to improve social, psychological health. and spirituality of the patient as an independent effort of the patient in helping his recovery.
Publisher: Lembaga Layanan Pendidikan Tinggi - LLDIKTI - Wilayah X
Date: 21-06-2022
Abstract: Penyakit hipertensi yang menjadi faktor utama kerusakan kardiovaskuler dapat memberikan d ak negatif pada semua organ terutama, jantung, ginjal bahkan serangan stroke secara tiba-tiba jika tidak segera teratasi dan membuat kualitas hidup penderita semakin memburuk. Penelitian ini bertujuan untuk mengeksplor pengalaman penderita hipertensi kronik dalam menjalani terapi bekam. Data didapatkan dengan melakukan wawancara kepada pasien hipertensi kronik sebanyak 5 partisipan yang didapatkan berdasarkan kriteria inklusi dengan menggunakan tehnik purposive s ling dengan menngunakan teknik collaizi dalam menganalisis hasil wawancara. Hasil yang didapatkan dalam penelitian ini dihasilkan 4 tema yakni 1) dimensi fisiologis berupa: nyeri yang berkurang/ hilang, mengurangi/ menghentikan konsumsi obat dan kualitas tidur meningkat, 2) dimensi psikologis berupa: perasaan senang, nyaman, stres dan cemas berkurang, 3) dimensi sosial berupa: dukungan keluarga dan 4) dimensi lingkungan berupa: keamanan dan pembiayaan yang lebih ekonomis. Terapi bekam memberikan pengaruh yang sangat baik dalam meningkatkan kualitas hidup penderita hipertensi kronik.
Publisher: Universitas Muhammadiyah Magelang
Date: 20-11-2022
DOI: 10.31603/NURSING.V9I2.7081
Abstract: Chronic non-communicable diseases have become a health concern in the health care system worldwide, especially in older people. Managing the disease was crucial to improving the quality of life among those older people. This study aims to identify self-management among older people with chronic non-communicable diseases. A cross-sectional study method was conducted in two primary healthcare centers. The respondents were recruited using accidental s ling, and 40 older people participated. The socio-demographics, lifestyle, and self-management were extracted by using paper-based questionnaires. All socio-demographic and lifestyle data statistically show no significant impact on the self-management of chronic non-communicable diseases among older people. Predominantly participants were old-old category (55%), women (62.5), the middle level of education (50%), household (42.5%), married (67.5%), consuming salted fish (80%), salted egg (50%). Since the study result is statistically insignificant, the large s le size may be needed to increase the study result. Keywords: Non-communicable disease community nursing elderly care quality of life self-management
Publisher: Universitas Imelda Medan
Date: 31-03-2023
DOI: 10.52943/JIKEPERAWATAN.V9I1.1201
Abstract: Prevalensi gangguan kesehatan mental terus meningkat setiap tahunnya di seluruh dunia. Rendahnya literasi kesehatan mental diidentifikasi menjadi penyebab utama meningkatnya prevalansi gangguan mental termasuk pada mahasiswa. Sebagian besar mahasiswa mengalami gangguan kesehatan mental tetapi kurang dari sebagian yang mendapatkan pelayanan bahkan tidak mengetahui bahwa mereka mengalami gangguan kesehatan mental. Penelitian ini bertujuan untuk mengetahui tingkat literasi mahasiswa terkait kesehatan mental agar menjadi masukan bagi institusi mengenai upaya peningkatan literasi mahasiswa terkhusus kesehatan mental. Penelitian ini menggunakan desain penelitian kuantitatif dengan pendekatan cross-sectional study. Studi yang dilakukan Universitas Hasanuddin sejak Mei s ai dengan Oktober 2022. Populasi penelitian ini adalah Mahasiswa rumpun Kesehatan di Universitas Hasanuddin, peserta dipilih dengan menggunakan metode purposive s ling. Pengumpulan data menggunakan kuesioner yang terdiri dari data sosiodemografi dan data riwayat literasi kesehatan mental. Hasil analisis pada penelitian ini diperoleh bahwa lebih dari sebagian mahasiswa Ilmu Keperawatan Universitas Hasanuddin memiliki tingkat literasi kesehatan mental yang tinggi yaitu 64.4%. Selain itu mahasiswa yang mengakses informasi mengenai kesehatan mental yang memiliki literasi kesehatan yang tinggi yaitu 71.2%. Hasil uji chi-square menunjukkan bahwa terdapat hubungan antara domisili dengan literasi kesehatan mental dengan p-value 0.025. Berdasarkan hasil penelitian, mahasiswa di Program Studi Ilmu Keperawatan di Universitas Hasanuddin memiliki literasi kesehatan mental yang tergolong tinggi serta terdapat hubungan antara asal domisili dengan literasi kesehatan mental. Kedepannya, dengan berlandaskan studi ini, diharapkan adanya penelitian lebih lanjut mengenai pengaruh variabel lain yang berhubungan dengan literasi kesehatan mental seperti budaya, dukungan keluarga, dukungan dari kelompok dan pertemanan.
Publisher: Lembaga Pendidikan Tinggi Wilayah 1 Sumatera Utara
Date: 10-2022
Abstract: Introduction: Breast cancer has been a new burden and health related issues around the world include Indonesia. The Indonesian Government introduced the cancer care day since the last decade. Healthcare volunteers (HCVs) have been incorporated in the national program under prima health care supervision. The role of HCVs is to assist health care professional and mediate the rural population to actively participate in the health program. This study aims to identify knowledge and practices on breast cancer prevention among rural HCVs. Methods: A cross-sectional study conducted with a total of 52 respondents form a sub-district in Sulawesi Tenggara, rural HCVs, aged 18 years and above, who were recruited voluntarily. Data collection using validated knowledge and practices on breast cancer prevention questionnaires. Results: The participation rate was 65% from total RHCVs, age mean score 35.9 (19-55), all respondents were women, Moslem (90%), was married (86%), and have children (84%), was 90% have an elementary and secondary level of education, and household (80%). Nearly half of the participants have inadequate knowledge on breast cancer prevention. Conclusion: The knowledge and practice among volunteers are remain lack. Study in advance to investigate determinants knowledge and practices on breast cancer program among rural health care volunteers in large s le size is needed.
Publisher: STIKES Aisyah Pringsewu Lampung
Date: 22-03-2021
Publisher: American Society for Microbiology
Date: 02-2014
DOI: 10.1128/JCM.03036-13
Abstract: Ureaplasma sp. infection in neonates and adults underlies a variety of disease pathologies. Of the two human Ureaplasma spp., Ureaplasma parvum is clinically the most common. We have developed a high-resolution melt (HRM) PCR assay for the differentiation of the four serovars of U. parvum in a single step. Currently U. parvum strains are separated into four serovars by sequencing the promoter and coding region of the multiple-banded antigen (MBA) gene. We designed primers to conserved sequences within this region for PCR lification and HRM analysis to generate reproducible and distinct melt profiles that distinguish clonal representatives of serovars 1, 3, 6, and 14. Furthermore, our HRM PCR assay could classify DNA extracted from 74 known (MBA-sequenced) test strains with 100% accuracy. Importantly, HRM PCR was also able to identify U. parvum serovars directly from 16 clinical swabs. HRM PCR performed with DNA consisting of mixtures of combined known serovars yielded profiles that were easily distinguished from those for single-serovar controls. These profiles mirrored clinical s les that contained mixed serovars. Unfortunately, melt curve analysis software is not yet robust enough to identify the composition of mixed serovar s les, only that more than one serovar is present. HRM PCR provides a single-step, rapid, cost-effective means to differentiate the four serovars of U. parvum that did not lify any of the known 10 serovars of Ureaplasma urealyticum tested in parallel. Choice of reaction reagents was found to be crucial to allow sufficient sensitivity to differentiate U. parvum serovars directly from clinical swabs rather than requiring cell enrichment using microbial culture techniques.
Publisher: Universitas Gadjah Mada
Date: 28-02-2022
Abstract: Latar Belakang: Penyakit kronis merupakan isu terkait kesehatan yang lazim ditemukan pada lansia yang dapat meningkatkan tingkat morbiditas dan mortalitas pasien lansia. Kualitas tidur pasien lansia dengan penyakit kronis dapat terganggu dan dapat menimbulkan efek buruk terhadap status kesehatan. Tujuan: Menganalisis kualitas tidur beserta determinannya pada pasien lanjut usia dengan penyakit kronis di RS Unhas Makassar. Metode: Penelitian ini dilakukan secara deskriptif analitik dengan metode survey cross-sectional pada pasien lanjut usia yang terdiagnosis penyakit kronis dan sedang dalam Perawatan di RS Unhas Makassarsebanyak 40 orang. Analisis data menggunakan uji Chi-Square dan uji Mann-whitney. Hasil: Hasil penelitian ini menunjukkan tidak ada pengaruh antara jumlah komorbit yang dimiliki, lama penyakit yang diderita, penggunaan obat-obatan serta status psikologis terhadap kualitas tidur pada usia lanjut. Satu-satunya risiko yang berpengaruh terhadap kualitas tidur pada penelitian ini adalah jenis kelamin dengan p value 0,049. Kualitas tidur yang buruk lebih sering terjadi ada lansia berjenis kelamin laki-laki dibandingkan dengan perempuan. Kesimpulan: Jenis kelamin merupakan prediktor terhadap kualitas tidur lansia, sedangkan faktor lainnya tidak menunjukkan signifikansinya. Untuk penelitian selanjutnya, maka penelitian dengan penetapan kriteria s el yang lebih selektif serta melibatkan jumlah responden yang lebih besar sangat dibutuhkan.
Publisher: The Korean Society of Critical Care Medicine
Date: 30-11-2022
Abstract: The provision of palliative care in the intensive care unit (ICU) is increasing. While some scholars have suggested the goals of palliative care to not be aligned with the ICU, some evidence show benefits of the integration. This review aimed to explore and synthesize research that identified barriers and facilitators in the provision of palliative care in the ICU. This review utilized Preferred Reporting Items for Systematic Reviews and Meta-Analyses Scoping Review guidelines based on population, concept, and context. We searched for eligible studies in five electronic databases (Scopus, PubMed, ProQuest, Science Direct, and Sage) and included studies on the provision of palliative care (concept) in the ICU (context) that were published in English between 2005–2021. We describe the provision of palliative care in terms of barriers and facilitators. We also describe the study design and context. A total of 14 papers was included. Several barriers and facilitators in providing palliative care in the ICU were identified and include lack of capabilities, family boundaries, practical issues, cultural differences. Facilitators of the provision of palliative care in an ICU include greater experience and supportive behaviors, i.e., collaborations between health care professionals. This scoping review demonstrates the breadth of barriers and facilitators of palliative care in the ICU. Hospital management can consider findings of the current review to better integrate palliative care in the ICU.
Publisher: Elsevier BV
Date: 08-2014
DOI: 10.1016/J.AJOG.2014.02.025
Abstract: Ureaplasma spp are the most commonly isolated microorganisms in association with preterm birth. Maternal erythromycin administration is a standard treatment for preterm prelabor rupture of membranes. There is little evidence of its effectiveness in eradicating Ureaplasma spp from the intrauterine cavity and fetus. We used a sheep model of intrauterine Ureaplasma spp infection to investigate the efficacy of repeated maternal intramuscular and intraamniotic erythromycin treatment to eradicate such an infection. Thirty ewes with singleton pregnancies received an intraamniotic injection of 10(7) color change units of erythromycin-sensitive Ureaplasma parvum serovar 3 at 55 days' gestation. At 116 days' gestation, 28 ewes with viable fetuses were randomized to receive (1) intraamniotic and maternal intramuscular saline solution treatment (n = 8), (2) single intraamniotic and repeated maternal intramuscular erythromycin treatment (n = 10), or (3) single maternal intramuscular and repeated intraamniotic erythromycin treatment (n = 10). Fetuses were surgically delivered at 125 days' gestation. Treatment efficacy was assessed by culture, quantitative polymerase chain reaction, and histopathologic evaluation. Animals treated with intraamniotic erythromycin had significantly less viable U parvum serovar 3 in the amniotic fluid at delivery. However, neither combination of maternal intramuscular and intraamniotic erythromycin treatment successfully cleared U parvum serovar 3 from the amniotic fluid or fetal tissues. Three de novo erythromycin-resistant U parvum isolates were identified in erythromycin-treated animals. Erythromycin treatment, given both to the ewe and into the amniotic cavity, fails to eradicate intrauterine and fetal U parvum serovar 3 infection and may lead to development of erythromycin resistant U parvum.
Publisher: American Society for Microbiology
Date: 11-2014
DOI: 10.1128/AAC.03721-14
Abstract: Treatment of intrauterine infection is likely key to preventing a significant proportion of preterm deliveries before 32 weeks of gestation. Azithromycin (AZ) may be an effective antimicrobial in pregnancy however, few gestation age-approriate data are available to inform the design of AZ-based treatment regimens in early pregnancy. We aimed to determine whether a single intra-amniotic AZ dose or repeated maternal intravenous (i.v.) AZ doses would safely yield therapeutic levels of AZ in an 80-day-gestation (term is 150 days) ovine fetus. Fifty sheep carrying single pregnancies at 80 days gestation were randomized to receive either: (i) a single intra-amniotic AZ administration or (ii) maternal intravenous AZ administration every 12 h. Amniotic fluid, maternal plasma, and fetal AZ concentrations were determined over a 5-day treatment regimen. Markers of liver injury and amniotic fluid inflammation were measured to assess fetal injury in response to drug exposure. A single intra-amniotic administration yielded significant AZ accumulation in the amniotic fluid and fetal lung. In contrast, repeated maternal intravenous administrations achieved high levels of AZ accumulation in the fetal lung and liver and a statistically significant increase in the fetal plasma drug concentration at 120 h. There was no evidence of fetal injury in response to drug exposure. These data suggest that (i) repeated maternal i.v. AZ dosing yields substantial fetal tissue uptake, although fetal plasma drug levels remain low (ii) transfer of AZ from the amniotic fluid is less than transplacental transfer and (iii) exposure to high concentrations of AZ did not elicit overt changes in fetal white blood cell counts, amniotic fluid monocyte chemoattractant protein 1 concentrations, or hepatotoxicity, all consistent with an absence of fetal injury.
Publisher: Elsevier BV
Date: 02-2015
DOI: 10.1016/J.AJOG.2015.09.076
Abstract: The preterm birth syndrome (delivery before 37 weeks gestation) is a major contributor to the global burden of perinatal morbidity and death. The cause of preterm birth is complex, multifactorial, and likely dependent, at least in part, on the gestational age of the fetus. Intrauterine infection is frequent in preterm deliveries that occur at <32 weeks gestation understanding how the fetus responds to proinflammatory insult will be an important step towards early preterm birth prevention. However, animal studies of infection and inflammation in prematurity commonly use older fetuses that possess comparatively mature immune systems. Aiming to characterize acute fetal responses to microbial agonist at a clinically relevant gestation, we used 92-day-old fetuses (62% of term) to develop a chronically catheterized sheep model of very preterm pregnancy. We hypothesized that any acute fetal systemic inflammatory responses would be driven by signaling from the tissues exposed to Escherichia coli lipopolysaccharide that is introduced into the amniotic fluid. Eighteen ewes that were carrying a single fetus at 92 days of gestation had recovery surgery to place fetal tracheal, jugular, and intraamniotic catheters. Animals were recovered for 24 hours before being administered either intraamniotic E coli lipopolysaccharide (n = 9) or sterile saline solution (n = 9). S les were collected for 48 hours before euthanasia and necroscopy. Fetal inflammatory responses were characterized by microarray analysis, quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay. Intraamniotic lipopolysaccharide reached the distal trachea within 2 hours. Lipopolysaccharide increased tracheal fluid interleukin-8 within 2 hours and generated a robust inflammatory response that was characterized by interleukin-6 signaling pathway activation and up-regulation of cell proliferation but no increases in inflammatory mediator expression in cord blood RNA. In very preterm sheep fetuses, lipopolysaccharide stimulates inflammation in the fetal lung and fetal skin and stimulates a systemic inflammatory response that is not generated by fetal blood cells. These data argue for amniotic fluid-exposed tissues that play a key role in driving acute fetal and intrauterine inflammatory responses.
Publisher: Poltekkes Kemenkes Kendari
Date: 30-04-2023
Abstract: Nelayan penyelam memiliki resiko gangguan kesehatan dan ancaman terhadap kecatatan dan bahkan kematian. Masalah yang lazim terjadi pada nelayan penyelam yaitu dekompresi dan barotrauma, dimana keduanya dapat menyebabkan masalah kesehatan yang fatal sehingga penelitian terkait upaya pencegahan dekompresi dan barotrauma menjadi penting untuk dilakukan. Penelitian ini bertujuan untuk mengetahui tingkat pengetahuan, sikap, dan praktik masyarakat nelayan terhadap upaya pencegahan dekompresi dan barotrauma di K ung Nelayan Untia Kota Makassar. Penelitian dilakukan dengan menggunakan metode cross-sectional study. Populasi dan s el merupakan masyarakat nelayan di K ung Nelayan Untia Kota Makassar. Hasil penelitian mengenai pencegahan dekompresi dan barotrauma di K ung Nelayan Untia Kota Makassar menunjukkan nelayan yang memiliki pengetahuan baik sebanyak 86%, sikap dengan kategori baik sebanyak 98%, dan praktik dengan kategori baik sebanyak 58%. Kemudian berbeda pada variabel sikap, ditemukan pengaruh antara pengetahuan dan praktik dengan penggunaan alat bantu napas sebagai upaya pencegahan dekompresi dan barotrauma nelayan di K ung Nelayan Untia Kota Makassar dengan p-value sebesar 0.003 dan 0.040. Hal ini disinyalir masih banyak faktor lain yang juga dapat mempengaruhi sikap nelayan antara lain pengalaman pribadi, emosional, bahkan kebudayaan.
Publisher: Wiley
Date: 14-11-2016
DOI: 10.1111/AJI.12599
Abstract: Complement is a central defence against sepsis, and increasing complement insufficiency in neonates of greater prematurity may predispose to increased sepsis. Ureaplasma spp. are the most frequently cultured bacteria from preterm blood s les. A sheep model of intrauterine Ureaplasma parvum infection was used to examine in vivo Ureaplasma bacteraemia at early and late gestational ages. Complement function and Ureaplasma killing assays were used to determine the correlation between complement potency and bactericidal activity of sera ex vivo. Ureaplasma was cultured from 50% of 95-day gestation lamb cord blood s les compared to 10% of 125-day gestation lambs. Bactericidal activity increased with increased gestational age, and a direct correlation between functional complement activity and bactericidal activity (R Ureaplasma bacteraemia in vivo was confined to early preterm lambs with low complement function, but Ureaplasma infection itself did not diminish complement levels.
Location: Indonesia
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Start Date: 2013
End Date: 2013
Funder: Australia Awards
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