ORCID Profile
0000-0002-1363-9562
Current Organisation
Federation University
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Publisher: Wiley
Date: 02-08-2012
Abstract: A series of Eu and Tb complexes of four different chiral ligands incorporating an azaxanthone sensitiser has been evaluated as probes for the bicarbonate anion. Their binding affinities were assessed at ambient pH with bicarbonate, lactate, citrate, phosphate and serum albumin. Binding was signalled by modulation of circularly polarised luminescence and apparent affinity constants were measured by examining changes in emission intensity ratios. Competition experiments show that with these species and ATP present at normal physiological values, bicarbonate can be determined selectively over the concentration range 10 to 35 mM. Bicarbonate levels are also reported by using a mixture of Eu and Tb complexes of a common ligand, examining the ratio of red/green emitted light. These methods have been adapted for the determination of bicarbonate in human serum and used for the assessment of mitochondrial levels of bicarbonate in several different cell types with confocal microscopy.
Publisher: Elsevier BV
Date: 04-2010
DOI: 10.1016/J.CBPA.2009.10.003
Abstract: Useful probes of the intracellular environment are required for a wide range of bioactive species including metal ions, oxyanions and pH. These probes need to be targeted to specific organelles (mitochondria, nucleus and lysosomes) in order to allow direct observation of the changes in these regions. Critical probe design features for luminescent lanthanide complexes are defined, together with a review of published sub-cellular localisation profiles. Cell uptake by macropinocytosis has been demonstrated for a wide range of probes and the importance of minimising perturbation of cellular homeostasis emphasised, so that cell viability, proliferation and membrane permeability are not compromised.
Publisher: Royal Society of Chemistry (RSC)
Date: 2008
DOI: 10.1039/B802838A
Abstract: Experimental measurements and theoretical analysis of magnetic properties, structural dynamics and acid-base equilibria for several lanthanide(III) complexes with tetraazacyclododecane derivatives as 19F NMR chemical shift pH probes are presented pKa values vary between 6.9 and 7.7, with 18 to 40 ppm chemical shift differences between the acidic and basic forms for Ho(III) complexes possessing T1 values of 10 to 30 ms (4.7-9.4 T, 295 K).
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C8AN01747F
Abstract: A thiocoumarin that exhibits varying fluorescence responses to metal ions in different solvents can be used in a single-probe multiple-solvent array for distinguishing metal ions.
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6CC03680E
Abstract: Pyrophosphate recognition using indicator displacement assays is compared to that of analogous receptors with covalently attached indicators.
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2CC34267G
Abstract: Europium and terbium complexes of two structurally related ligands have been evaluated as optical probes to monitor changes in lysosomal pH calibration using ionophores and fluorescent probes allows monitoring of the time dependence of lysosomal pH change, examining the green/red intensity ratio from internalised Tb-Eu complexes.
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7AN01200D
Abstract: A review of fluorescent sensing arrays for anions and cations, highlighting promising strategies and directions for future research.
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C1CC11853F
Abstract: Image and spectral intensity from bicarbonate-selective europium(III) probes localised in the mitochondria of cells is modulated reversibly by variation of external pCO(2), and is suppressed by addition of the carbonic anhydrase inhibitor, acetazolomide.
Publisher: Springer Science and Business Media LLC
Date: 28-09-2017
DOI: 10.1038/S41467-017-00852-6
Abstract: Advances in bottom-up material design have been significantly progressed through DNA-based approaches. However, the routine integration of semiconducting properties, particularly long-range electrical conduction, into the basic topological motif of DNA remains challenging. Here, we demonstrate this with a coordination polymer derived from 6-thioguanosine (6-TG-H), a sulfur-containing analog of a natural nucleoside. The complexation reaction with Au(I) ions spontaneously assembles luminescent one-dimensional helical chains, characterized as {Au I (μ-6-TG)} n , extending many μm in length that are structurally analogous to natural DNA. Uniquely, for such a material, this gold-thiolate can be transformed into a wire-like conducting form by oxidative doping. We also show that this self-assembly reaction is compatible with a 6-TG-modified DNA duplex and provides a straightforward method by which to integrate semiconducting sequences, site-specifically, into the framework of DNA materials, transforming their properties in a fundamental and technologically useful manner.
Publisher: Royal Society of Chemistry (RSC)
Date: 2016
DOI: 10.1039/C6AN00510A
Abstract: A four-membered array based on fluorescent thiophenes is capable of distinguishing transition metal ions.
No related grants have been discovered for David Smith.