ORCID Profile
0000-0002-6233-5143
Current Organisation
Universiti Putra Malaysia
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Publisher: MDPI AG
Date: 23-11-2022
Abstract: This current investigation was undertaken both in laboratory and glasshouse for documentation and quantification of phytochemicals from different parts of the parthenium (Parthenium hysterophorus L.) plant through LC-MS and HPLC to study their effect on two crops namely, Bambara groundnut (Vigna subterranean L.) and maize (Zea mays L.), and six different types of weed e.g., Digitaria sanguinalis, Eleusine indica, Ageratum conyzoides, Cyperus iria, Euphorbia hirta, and Cyperus difformis. The parthenium methanolic leaf extracts at 25, 50, 75, and 100 g L−1 were sprayed in the test crops and weeds to assess their physiological and biochemical reactions after 6, 24, 48, and 72 h of spraying these compounds (HAS). The LC-MS analysis confirmed seven types of phytochemicals (caffeic acid, ferulic acid, vanillic acid, parthenin, chlorogenic acid, quinic acid, and p-anisic acid) in the parthenium leaf extract that were responsible for the inhibition of tested crops and weeds. From the HPLC analysis, higher amounts in leaf methanol extracts (40,752.52 ppm) than those of the stem (2664.09 ppm) and flower extracts (30,454.33 ppm) were recorded. Parthenium leaf extract at 100 g L−1 had observed higher phytotoxicity on all weed species except C. difformis. However, all crops were found safe under this dose of extraction. Although both crops were also affected to some extent, they could recover from the stress after a few days. The photosynthetic rate, transpiration rate, stomatal conductance, carotenoid and chlorophyll content were decreased due to the application of parthenium leaf extract. However, when parthenium leaf extract was applied at 100 g L−1 for 72 h, the malondialdehyde (MDA) and proline content were increased in all weeds. Enzymatic antioxidant activity (e.g., superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) contents) were also elevated as a result of the sprayed parthenium leaf extract. The negative impact of physiological and biochemical responses as a consequence of the parthenium leaf extract led the weed species to be stressed and finally killed. The current findings show the feasibility of developing bioherbicide from the methanolic extract of parthenium leaf for controlling weeds, which will be cost-effective, sustainable, and environment friendly for crop production during the future changing climate.
Publisher: MDPI AG
Date: 08-09-2021
DOI: 10.3390/AGRICULTURE11090856
Abstract: Parthenium weed is an invasive weed species of economic importance worldwide. Native to the American tropics, the infestation ability of Parthenium weed to a new habitat is largely influenced by environmental factors. Despite Parthenium weed invasion in Malaysia dated back to 2013, investigation on its ecological behavior is still lacking. Hence, extensive studies on the ecological behavior of two predominant Malaysian Parthenium weed populations were executed. In the Petri dish seed bioassay, germination of Parthenium weed seeds was evident at temperatures up to 80 °C. Parthenium weed was also germinable in saline condition of up to 250 mM, osmotic pressure ranging from −1.2 to 0 MPa, and a wide range of pH (4–9), thus these abiotic conditions are by no means the limiting factors for the Parthenium weed. The pot trial observed that this invasive weed grew readily in various Malaysian soil textures. Parthenium weed successfully emerged from 0 cm to not beyond 2 cm soil burial and retained its emergence capacity under different submergence periods in water. The most favorable soil moisture condition for Parthenium weed emergence was saturated (0 kPa), followed by field capacity (−30–−50 kPa), while no emergence occurred in drought (−70 kPa) as well as flooded soils. These indicate that both Parthenium weed populations possess high tolerance to various abiotic conditions in Malaysia. Results obtained in the current study have crucially become guidelines for the local government authorities in predicting wide spread of Parthenium weed in erse ecological zones, to further manage this pernicious weed efficiently.
Publisher: MDPI AG
Date: 27-07-2021
Abstract: Plant-based bioherbicides could be an effective alternative to current chemical herbicides for sustainable agriculture. Therefore, this research evaluated the weed control efficacy and crop-weed selectivity of the new plant-derived bioherbicide WeedLock compared to commercial herbicides in glasshouse and field conditions. In the glasshouse, the herbicides applied were WeedLock (672.75, 1345.50, 2691.00 L ha−1), glyphosate isopropyl-amine, and glufosinate-ammonium (1, 2, 4 L ha−1), over the untreated (control) on six weeds and four crops. In the field trial, typical weeds were allowed to grow at a uniform density across plots (2.5 × 2.5 m), and WeedLock (1345.50, 2691.00 L ha−1), glyphosate isopropyl-amine, and glufosinate-ammonium (2, 4 L ha−1) were applied along with untreated plot (control). A randomized complete block design was set with four replications for glasshouse and field experiments. WeedLock at 1345.50 L ha−1 showed efficacy similar to glyphosate isopropyl-amine and glufosinate-ammonium at 2 L ha−1 for Ageratum conyzoides L. in the glasshouse. Applied herbicides killed all tested crops except Zea mays L. at 1345.50 L ha−1 (WeedLock). WeedLock showed more than 50% efficacy at 35 days after spray, while 65% was produced by glyphosate isopropyl-amine and glufosinate-ammonium compared to the untreated plot (control). WeedLock has excellent potential to control weeds in both glasshouse and field conditions and showed a non-selective character.
Publisher: MDPI AG
Date: 14-06-2021
Abstract: The current study was designed to investigate the effect of Parthenium hysterophorus L. methanol extract on Ageratum conyzoides L., Oryza sativa f. spontanea (weedy rice) and Cyperus iria L. in glasshouse condition. Here, Parthenium hysterophorus methanol extract at 20, 40, and 60 g L−1 concentrations was applied on the test species to examine their physiological and biochemical responses at 6, 24, 48 and 72 h after spraying (HAS). The phytotoxicity of P. hysterophorus was strong on A. conyzoides compared to weedy rice and Cyperus iria at different concentrations and exposure times. There was a reduction in photosynthesis rate, stomatal conductance, transpiration, chlorophyll content and carotenoid content when plants were treated with P. hysterophorus extract concentrations. Exposure to P. hysterophorus (60 g L−1) at 24 HAS increased malondialdehyde (MDA) and proline content by 152% and 130%, respectively, in A. conyzoides compared with control. The activities of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD)) were also increased in the presence of P. hysterophorus extract. Present findings confirm that the methanol extract of P. hysterophorus can disrupt the physiological and biochemical mechanism of target weeds and could be used as an alternative to chemical herbicides.
Publisher: MDPI AG
Date: 29-07-2021
Abstract: Parthenium hysterophorus is an invasive weed species that competes aggressively with other plants and is also allelopathic. It poses a significant risk to human health, livestock, the environment, soil, and agriculture. However, given some clinical studies, its potential for antidiabetic, antioxidant, antitumor, herbicidal, pesticidal, and antimalarial therapies should be researched further in attempts to discover more relevant applications. It can be used as a nutrient-dense, readily available, and cheap fertilizer. Parthenium can also be used as an herbicide, an insecticide, and a phyto-remedial mediator to extract metals and dyes from agricultural waste. Here we provide basic information on the morphology, reproduction, environmental impacts, and management of this species. Effects of methanol, ethanol, hexane, acetone, and aqueous (water) Parthenium extracts are described. Because P. hysterophorus is said to be one of the world’s seven worst weeds, some control measures, including mechanical, chemical, cultural, and biological control, are discussed. The allelopathy of this weed is difficult to regulate, and there are both positive and negative interactions between Parthenium and other species due to allelochemical action. Several toxic phenolic compounds produced by P. hysterophorus are responsible for weed suppression, and we discuss details of their mode of action and potential applications.
Publisher: Springer Science and Business Media LLC
Date: 17-04-2021
Publisher: Cambridge University Press (CUP)
Date: 20-04-2021
DOI: 10.1017/WSC.2021.33
Abstract: Weedy rice ( Oryza sativa f. spontanea or O. sativa complex) has become a severe threat to Malaysian rice ( Oryza sativa L.) granaries after the direct-seeding method of rice cultivation was introduced in the late 1980s. Since then, researchers have studied the biology and ecology of weedy rice and espoused the evolutionary theory of the origin of Malaysian weedy rice. This review paper aimed to synthesize the body of knowledge about weedy rice and the evolution of herbicide-resistant (HR) weedy rice in Malaysia. The imidazolinone (IMI) herbicide component of the Clearfield® Production System (CPS) rice package is among the most effective tools for weedy rice control. However, dependence solely on this technology and farmers’ ignorance about the appropriate use of IMI herbicides with the CPS rice package have resulted in the evolution of IMI-resistant (IMI-R) weedy rice. This has reduced the efficacy of IMI herbicides on weedy rice, ultimately nullifying the benefit of CPS rice in affected fields. At present, it is assumed that IMI-R weedy rice populations are widely distributed across the rice granaries in Malaysia. Therefore, it is important that integrated management measures be adopted comprehensively by Malaysian rice growers to curb the spread of IMI-R weedy rice problem in Malaysia, especially in fields planted with CPS rice. This review focuses on the biology of Malaysian weedy rice, the history of the establishment of weedy rice in Malaysian rice fields, the impact of HR rice technology on the evolution of IMI-R weedy rice in Malaysia, the distribution of resistant weedy rice populations across Peninsular Malaysia rice granaries, the weedy rice resistance mechanisms, and weedy rice management. The synthesis of all this information is helpful to researchers, policy makers, the private agricultural industry, advisers to farmers, and proactive farmers themselves with the goal of working toward sustainable rice production.
Publisher: MDPI AG
Date: 15-06-2021
Abstract: Weed management is an arduous undertaking in crop production. Integrated weed management, inclusive of the application of bioherbicides, is an emerging weed control strategy toward sustainable agriculture. In general, bioherbicides are derived either from plants containing phytotoxic allelochemicals or certain disease-carrying microbes that can suppress weed populations. While bioherbicides have exhibited great promise in deterring weed seed germination and growth, only a few in vitro studies have been conducted on the physiological responses they evoke in weeds. This review discusses bioherbicide products that are currently available on the market, bioherbicide impact on weed physiology, and potential factors influencing bioherbicide efficacy. A new promising bioherbicide product is introduced at the end of this paper. When absorbed, phytotoxic plant extracts or metabolites disrupt cell membrane integrity and important biochemical processes in weeds. The phytotoxic impact on weed growth is reflected in low levels of root cell ision, nutrient absorption, and growth hormone and pigment synthesis, as well as in the development of reactive oxygen species (ROS), stress-related hormones, and abnormal antioxidant activity. The inconsistency of bioherbicide efficacy is a primary factor restricting their widespread use, which is influenced by factors such as bioactive compound content, weed control spectrum, formulation, and application method.
Publisher: MDPI AG
Date: 04-08-2023
Abstract: The need to meet food demand becomes more urgent as it is forecasted to increase by 50% over the next century. Thus, agronomists promote sensible tools and approaches to eradicate factors that h er crop production, mainly weeds. The constant use of chemical herbicides to control weeds leads to an increased risk of herbicide-resistant weed populations, environmental pollution, unsafe agricultural products, and negative effects on human health. These problems have caused an interest among researchers to replace synthetic herbicides with alternatives. The purpose of this review was to present the current knowledge base on allelopathic tropical plants and their potential for use in the development of natural product-based, environmentally friendly herbicides for sustainable agriculture, and to stimulate future discussion on this topic. The defence mechanisms of tropical plants have received particular attention because of their potential weed control ability as a natural pesticide that can prevent the overuse of synthetic pesticides. The ancient knowledge of the toxic properties of various tropical plants gives us a basis for creating a novel pest control approach. The synthesis of biopesticides based on allelochemicals opens up the possibility of utilizing natural compounds in crop protection and demonstrates the ability to deal with evolved pesticide resistance.
Publisher: Public Library of Science (PLoS)
Date: 14-09-2020
Publisher: MDPI AG
Date: 18-08-2022
Abstract: The utilization of the invasive weed, Parthenium hysterophorus L. for producing value-added products is novel research for sustaining our environment. Therefore, the current study aims to document the phytotoxic compounds contained in the leaf of parthenium and to examine the phytotoxic effects of all those phytochemicals on the seed sprouting and growth of Crabgrass Digitaria sanguinalis (L.) Scop. and Goosegrass Eleusine indica (L.) Gaertn. The phytotoxic substances of the methanol extract of the P. hysterophorus leaf were analyzed by LC-ESI-QTOF-MS=MS. From the LC-MS study, many compounds, such as terpenoids, flavonoids, amino acids, pseudo guaianolides, and carbohydrate and phenolic acids, were identified. Among them, seven potential phytotoxic compounds (i.e., caffeic acid, vanillic acid, ferulic acid, chlorogenic acid, quinic acid, anisic acid, and parthenin) were documented, those are responsible for plant growth inhibition. The concentration needed to reach 50% growth inhibition in respect to germination (ECg50), root length (ECr50), and shoot length (ECs50) was estimated and the severity of phytotoxicity of the biochemicals was determined by the pooled values (rank value) of three inhibition parameters. The highest growth inhibition was demarcated by caffeic acid, which was confirmed and indicated by cluster analysis and principal component analysis (PCA). In the case of D. sanguinalis, the germination was reduced by 60.02%, root length was reduced by 76.49%, and shoot length was reduced by 71.14% when the chemical was applied at 800 μM concentration, but in the case of E. indica, 100% reduction of seed germination, root length, and shoot length reduction occurred at the same concentration. The lowest rank value was observed from caffeic acids in both E. indica (rank value 684.7) and D. sanguinalis (909.5) caused by parthenin. It means that caffeic acid showed the highest phytotoxicity. As a result, there is a significant chance that the parthenium weed will be used to create bioherbicides in the future.
Publisher: Cold Spring Harbor Laboratory
Date: 23-01-2022
DOI: 10.1101/2022.01.21.477310
Abstract: Herbicides made from natural molecules may be a good environmentally friendly alternative to synthetic chemical herbicides for weed control. As a result, this investigation was carried out to ascertain the phytotoxicity of Parthenium hysterophorus L. as well as to identify its phenolic components. Germination of seeds and development of seedlings of Vigna subterranea (L.) Verdc, Raphanus sativus (L.) Domin, Cucurbita maxima Duchesne., Cucumis sativus L., Solanum lycopersicum L., Capsicum frutescens L., Zea mays L., Abelmoschus esculentus (L.) Moench, Daucus carota L., Digitaria sanguinalis (L.) Scop and Eleusine indica (L.) Gaertn were investigated using P. hysterophorus leaf, stem, and flower methanol extracts. Six concentrations (25, 50, 75, 100, and 150 g L −1 ) were comparison to the control (distilled water). The concentration of extracts increased, the rate of the seed sprouting and seedling growth decreased. EC 50 values showed that the extraction of leaf of P. hysterophorus (811) was phytotoxic in comparison to the stem (1554) and flower (1109) extract. According to PCA analysis, Raphanus sativus, Solanum lycopersicum, Capsicum frutescens, Abelmoschus esculentus, Daucus carota, Digitaria sanguinalis , and Eleusine indica were all very susceptible to allelochemicals. A LC-MS analysis revealed that the P. hysterophorus leaf extract contained 7 phenolic compounds that were responsible for inhibition. These studies also revealed that the leaf of P. hysterophorus is a major source of phytotoxicity, which could be valuable in the future for developing a natural herbicide.
Publisher: Cold Spring Harbor Laboratory
Date: 19-12-2019
DOI: 10.1101/2019.12.19.882159
Abstract: The IMI-herbicides rice package has been recognized by all means among the most efficient chemical approaches for weedy rice control nowadays. Inevitably, the continuous and sole dependence, as well as ignorance on the appropriate use of imidazolinone herbicides in the IMI-herbicides rice package by rice growers has caused the development of herbicide resistance in weedy rice populations across many IMI-herbicides rice package adopted countries, inclusive of Malaysia. Hence, a comprehensive study was conducted to elucidate the occurrence, level, and mechanisms endowing resistance to IMI-herbicides on field-reported resistant (R) weedy rice populations collected from IMI-rice fields in K ung Simpang Sanglang, Perlis (A), K ung Behor Mentalon, Perlis (B), and K ung Sungai Kering, Kedah (C). The collected weedy rice populations were compared with a susceptible weedy rice population (S), an imidazolinone-resistant rice cultivar (IMI-rice), and a susceptible local rice cultivar (MR219). Dose-response experiments were carried out using commercial IMI-herbicides (premix of imazapic and imazapyr) available in the IMI-herbicides rice package, in the seed bioassay and whole-plant dose-response. Based on the Resistance Index (RI) quantification in both experiments, the cross-resistance pattern of weedy rice populations and rice varieties to imazapic and imazapyr was determined. Molecular investigation was carried out by comparing acetohydroxyacid synthase (AHAS) gene sequences between resistant (R) weedy rice populations (A, B, and C), S population, IMI-rice, and MR219. Evidently, the AHAS gene sequences of R weedy rice were identical to the IMI-rice, revealing that amino acid substitution of Ser-653-Asn occurs in both R populations and IMI-rice, but neither in MR219 nor S plants. In vitro assays were conducted using analytical grade imidazolinone herbicides of imazapic (99.3%) and imazapyr (99.6%) with seven concentrations. The results demonstrated that the AHAS enzyme extracted from R populations and IMI-rice were less sensitive to IMI-herbicides in comparison to S and MR219, further supporting the IMI-herbicides resistance was conferred by target site mutation. In conclusion, the basis of imidazolinone resistance in selected populations of Malaysia weedy rice was due to a Ser-653-Asn mutation that reduced sensitivity of the target site to IMI-herbicides. The current study presents the first report of resistance mechanism in weedy rice in Malaysian rice fields.
Publisher: MDPI AG
Date: 15-07-2021
Abstract: The allelopathic effect of various concentrations (0, 6.25, 12.5, 50 and 100 g L−1) of Parthenium hysterophorus methanol extract on Cyperus iria was investigated under laboratory and glasshouse conditions. No seed germination was recorded in the laboratory when P. hysterophorus extract was applied at 50 g L−1. In the glasshouse, C. iria was mostly injured by P. hysterophorus extract at 100 g L−1. The phytochemical constituents of the methanol extract of P. hysterophorus were analyzed by LC-ESI-QTOF-MS=MS. The results indicated the presence of phenolic compounds, terpenoids, alkaloids, amino acids, fatty acids, piperazines, benzofuran, indole, amines, azoles, sulfonic acid and other unknown compounds in P. hysterophorus methanol extract. A comparative study was also conducted between P. hysterophorus extract (20, 40 and 80 g L−1) with a synthetic herbicide (glyphosate and glufosinate ammonium at 2 L ha−1) as a positive control and no treatment (negative control) on Ageratumconyzoides, Oryzasativa and C. iria. The growth and biomass of test weeds were remarkably inhibited by P. hysterophorus extract. Nevertheless, no significant difference was obtained when P. hysterophorus extract (80 g L−1) and synthetic herbicides (glyphosate and glufosinate ammonium) were applied on A.conyzoides.
No related grants have been discovered for Muhammad Saiful Ahmad Hamdani.