ORCID Profile
0000-0002-9942-4502
Current Organisations
International Iberian Nanotechnology Laboratory
,
University of Oxford
Does something not look right? The information on this page has been harvested from data sources that may not be up to date. We continue to work with information providers to improve coverage and quality. To report an issue, use the Feedback Form.
Publisher: Wiley
Date: 14-11-2022
Abstract: In the context of personalized medicine, the analysis of single cells is key in order to understand the origin and evolution of cancer to provide an accurate prognosis. Microfluidics and microdroplets are increasingly used for the handling and understanding of the behavior of single cells, as they offer the perfect isolated environment. However, due to the small volumes handled, it is necessary to couple this technology with an ultrasensitive detection technique. Herein, surface‐enhanced Raman scattering (SERS) spectroscopy and droplet microfluidics are combined toward the multiplex phenotypic analysis of single cancer cells. For this, cancer cells are labeled with different SERS tags that recognize membrane proteins and encapsulated in idually in microdroplets. Afterward, single cells within microdroplets are imaged by SERS spectroscopy. To the best of the authors’ knowledge, this is the first time that a multiplex phenotypic SERS analysis of single cells in microdroplets is shown. This integrated optofluidic platform paves the way toward the multiplex and automated characterization of cell populations in cancer patients.
Publisher: Springer Science and Business Media LLC
Date: 03-02-2020
DOI: 10.1007/S00429-020-02029-2
Abstract: In the hippoc al CA1 area, the GABAergic trilaminar cells have their axon distributed locally in three layers and also innervate the subiculum. Trilaminar cells have a high level of somato-dendritic muscarinic M2 acetylcholine receptor, lack somatostatin expression and their presynaptic inputs are enriched in mGluR8a. But the origin of their inputs and their behaviour-dependent activity remain to be characterised. Here we demonstrate that (1) GABAergic neurons with the molecular features of trilaminar cells are present in CA1 and CA3 in both rats and mice. (2) Trilaminar cells receive mGluR8a-enriched GABAergic inputs, e.g. from the medial septum, which are probably susceptible to hetero-synaptic modulation of neurotransmitter release by group III mGluRs. (3) An electron microscopic analysis identifies trilaminar cell output synapses with specialised postsynaptic densities and a strong bias towards interneurons as targets, including parvalbumin-expressing cells in the CA1 area. (4) Recordings in freely moving rats revealed the network state-dependent segregation of trilaminar cell activity, with reduced firing during movement, but substantial increase in activity with prolonged burst firing ( 200 Hz) during slow wave sleep. We predict that the behaviour-dependent temporal dynamics of trilaminar cell firing are regulated by their specialised inhibitory inputs. Trilaminar cells might support glutamatergic principal cells by disinhibition and mediate the binding of neuronal assemblies between the hippoc us and the subiculum via the transient inhibition of local interneurons.
Publisher: Society for Neuroscience
Date: 29-03-2019
Publisher: Springer Science and Business Media LLC
Date: 03-03-2018
Location: United Kingdom of Great Britain and Northern Ireland
No related grants have been discovered for Linda Katona.