ORCID Profile
0000-0002-2068-7669
Current Organisation
Universidad Católica de la Santísima Concepción
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Publisher: Elsevier BV
Date: 15-10-2007
Publisher: Wiley
Date: 09-07-2014
DOI: 10.1002/YEA.3025
Abstract: Metabolic engineering of microbial strains to produce aromatic compounds deriving from the shikimate pathway is of great interest to the chemical industry as a more sustainable alternative for feedstock production. Chorismate is a significant intermediate in the shikimate pathway. In this study, the formation of phenylalanine and phenylpyruvate as by-products in strains engineered downstream of the chorismate node for increased aromatic production was explored in yeast fermentations. Tracer experiments showed that these compounds are synthesized de novo during fermentation, under conditions in which their synthesis was genetically blocked. Chorismate stability evaluation, as well as deletion mutation analysis throughout the phenylalanine biosynthesis pathway, suggested that this synthesis was a result of intracellular, non-enzymatic rearrangement of chorismate to phenylpyruvate via prephenate, which was followed by enzymatic transamination of phenylpyruvate to form phenylalanine. These results not only aid in the development of strain-engineering strategies to avoid the accumulation of by-products during fermentations aimed at increased aromatics production, but also deepen our understanding of yeast metabolism.
Publisher: Elsevier BV
Date: 03-2020
Publisher: Elsevier BV
Date: 11-2018
Publisher: Springer Science and Business Media LLC
Date: 07-03-2019
Publisher: Elsevier BV
Date: 03-2019
DOI: 10.1016/J.MARPOLBUL.2019.01.025
Abstract: In this study we have identified and characterized microplastic particles (MPs) found in six fish species of commercial importance in central Chile. The fish species belong to different trophic levels and were obtained from the oceanic and coastal habitats. To analyze MPs, the fish gastrointestinal content was extracted, analyzed and characterized using a microscopy equipped with Fourier-transform infrared spectroscopy (FT-IR). The MPs found in fish s les were mainly constituted by red microfibers (70-100%) with sizes ranging between 176 and 2842 μm. Polyester, polyethylene (PE) and polyethylene terephthalate (PET) were identified as the prevalent polymers detected. The coastal species showed the presence of microfibers with a higher size and abundance (71%) compared to oceanic species (29%), suggesting there is a greater exposure risk. These findings are consistent with results found in other investigations worldwide. However, further research is still needed to accurately establish the potential exposure risk for the public consuming these fish and the impact of MPs in the Chilean fishery activities.
Publisher: Elsevier BV
Date: 2013
DOI: 10.1016/J.JBIOTEC.2012.04.014
Abstract: Aromatics are amongst the most important bulk feedstocks for the chemical industry, however, no viable bioprocess exists today and production is still dependent on petro-chemistry. In this article the production of aromatic precursors such as p-hydroxybenzoic acid (PHBA) and p-amino benzoic acid (PABA) in Saccharomyces cerevisiae was evaluated using metabolic network analysis. Theoretical mass yields for PHBA and for PABA obtained by metabolic network analysis were 0.58 and 0.53 g g(glucose)⁻¹, respectively. A major setback for microbial production of aromatics is the high toxicity of the products. Therefore, PHBA and PABA toxicity was evaluated in S. cerevisiae. Minimal inhibitory concentrations of 38.3 g L⁻¹ for PHBA and 0.62 g L⁻¹ for PABA were observed. However, PABA toxicity could be alleviated in adaptation experiments. Finally, metabolic engineering was used to create proof of principle first generation strains of S. cerevisiae. Overall accumulation of 650 μM PHBA and 250 μM PABA could be achieved.
Publisher: Elsevier BV
Date: 02-2019
Publisher: Elsevier BV
Date: 2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9RA00536F
Publisher: Elsevier BV
Date: 12-2020
Publisher: Elsevier BV
Date: 02-2020
Publisher: Elsevier BV
Date: 10-2005
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9RA04198B
Publisher: Elsevier BV
Date: 02-2020
Publisher: Wiley
Date: 28-02-2005
DOI: 10.1002/BIT.20443
Publisher: Elsevier BV
Date: 07-2023
Publisher: Elsevier BV
Date: 05-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8RA01887A
Publisher: Springer Science and Business Media LLC
Date: 03-01-2023
No related grants have been discovered for Dariela Núñez.