ARDC Research Link Australia

ORCID Profile
Orcid icon. 0000-0002-8391-5462

Current Organisations
University College London , Centre Hospitalier Universitaire Vaudois , Great Ormond Street Hospital For Children NHS Foundation Trust , Sainsbury-Wellcome Centre for Neural Circuits and Behaviour

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Publications

Publication

A deep learning algorithm for 3D cell detection in whole mouse brain image datasets

Publisher: Public Library of Science (PLoS)

Date: 28-05-2021

DOI: 10.1371/JOURNAL.PCBI.1009074

Abstract: Understanding the function of the nervous system necessitates mapping the spatial distributions of its constituent cells defined by function, anatomy or gene expression. Recently, developments in tissue preparation and microscopy allow cellular populations to be imaged throughout the entire rodent brain. However, mapping these neurons manually is prone to bias and is often impractically time consuming. Here we present an open-source algorithm for fully automated 3D detection of neuronal somata in mouse whole-brain microscopy images using standard desktop computer hardware. We demonstrate the applicability and power of our approach by mapping the brain-wide locations of large populations of cells labeled with cytoplasmic fluorescent proteins expressed via retrograde trans-synaptic viral infection.

Publication

Consequences of gestational diabetes mellitus on neonatal cardiovascular health: MySweetHeart Cohort study

Publisher: Springer Science and Business Media LLC

Date: 28-11-2022

DOI: 10.1038/S41390-022-02390-4

Abstract: Hyperglycaemic disorders of pregnancy are associated with offspring cardiovascular alterations. MySweetHeart cohort study aimed to assess the effect of maternal gestational diabetes (GDM) on offsprings’ cardiovascular health. Newborns underwent clinical and echocardiographic examinations between 2016 and 2020. Compared to mothers without GDM ( n = 141), mothers with GDM ( n = 123) were more likely to have had GDM in previous pregnancies and had higher weight, BMI, blood glucose, and HbA1c. Newborns of both groups showed similar clinical characteristics. Echocardiography was performed on the 3rd (interquartile range, IQR, 2nd–4th) day of life in 101 offsprings of mothers without and 116 offsprings of mothers with GDM. Left ventricular (LV) mass was similar. Children born to mothers with GDM had a thicker posterior LV wall ( z -score +0.15, IQR –0.38/0.62, versus +0.47, IQR –0.11/+1.1, p = 0.004), a smaller end-systolic (1.3 mL, IQR 1.0–1.5 mL, versus 1.4 mL, IQR 1.2–1.8 mL, p = 0.044) but a similar end-diastolic LV volume. They also had shorter tricuspid valve flow duration and aortic valve ejection time, lower tricuspid E-wave and pulmonary valve velocities. Newborns of mothers with or without GDM had similar clinical characteristics and LV mass. However, some echocardiographic differences were detected, suggesting an altered myocardial physiology among infants of mothers with GDM. ClinicalTrials.gov (NCT02872974). Hyperglycaemic disorders of pregnancy are known to be associated with offspring cardiovascular alterations. Clinical characteristics and estimated left ventricular (LV) mass were similar in children issued from mothers with and without gestational diabetes (GDM). Children born to mothers with GDM had a thicker posterior LV wall and a smaller end-systolic LV volume. Although LV mass is not different, myocardial physiology may be altered in these infants. Further studies should investigate the endothelial function of this population and the cardiovascular evolution of these children over time.