ORCID Profile
0000-0001-6171-5606
Current Organisations
Aalborg Universitet
,
Aalborg University
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Publisher: Oxford University Press (OUP)
Date: 03-2003
DOI: 10.1086/367663
Abstract: Serological analysis is often used for the diagnosis of chlamydial infections. However, an increase in Chlamydia antibodies has been reported in patients with parvovirus and Mycoplasma infections. Whether this antibody response is the result of dual infection or nonchlamydial antigen stimulation is unknown. In a randomized study, 48 healthy volunteers either were immunized against yellow fever, polio, diphtheria, and tetanus (the group receiving intervention with nonchlamydial antigen) or received saline injections (the placebo group). The change in antibody levels was compared between the 2 groups. The Chlamydia recombinant lipopolysaccharide enzyme-linked immunosorbent assay (Medac) showed an increase in the antibody titer in the intervention group, compared with that in the control group (for immunoglobulin M, P=.004 for immunoglobulin A, P=.038 and for immunoglobulin G, P=.056), but no differences between study groups was found when the C. pneumoniae enzyme immunoassay (EIA ThermoLabsystems), the C. pneumoniae EIA (Medac), and the microimmunofluorescence test (MRL) were used. An increase in antibodies to Chlamydia organisms can be measured after exposure to nonchlamydial antigens, depending on the test used.
Publisher: Springer Science and Business Media LLC
Date: 12-1991
DOI: 10.1007/BF01984929
Publisher: Springer Science and Business Media LLC
Date: 2003
Publisher: The Endocrine Society
Date: 08-2004
DOI: 10.1210/ER.2003-0012
Publisher: Springer Science and Business Media LLC
Date: 2002
Abstract: Chlamydia pneumoniae infection has been detected by serological methods, but PCR is gaining more interest. A number of different PCR assays have been developed and some are used in combination with serology for diagnosis. Real-time PCR could be an attractive new PCR method therefore it must be evaluated and compared to conventional PCR methods. We compared the performance of a newly developed real-time PCR with a conventional PCR method for detection of C. pneumoniae. The PCR methods were tested on reference s les containing C. pneumoniae DNA and on 136 nasopharyngeal s les from patients with chronic cough. We found the same detection limit for the two methods and clinical performance was equal for the real-time PCR and for the conventional PCR method, although only three s les tested positive. To investigate whether the low prevalence of C. pneumoniae among patients with chronic cough was caused by suboptimal PCR efficiency in the s les, PCR efficiency was determined based on the real-time PCR. Seventeen of twenty randomly selected clinical s les had similar PCR efficiency to s les containing pure genomic C. pneumoniae DNA. These results indicate that the performance of real-time PCR is comparable to that of conventional PCR, but this needs to be confirmed further. Real-time PCR can be used to investigate the PCR efficiency which gives a rough estimate of how well the real-time PCR assay work in a specific s le type. Suboptimal PCR efficiency of PCR is not a likely explanation for the low positivity rate of C. pneumoniae in patients with chronic cough.
No related grants have been discovered for Svend Birkelund.