ORCID Profile
0000-0002-6854-2100
Current Organisation
University of California, San Diego
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Publisher: Elsevier BV
Date: 07-2010
Publisher: Microbiology Society
Date: 09-2013
Abstract: Acinetobacter spp. are a erse group of Gram-negative bacteria frequently implicated in nosocomial infections. Genotypic methods have been instrumental in studying Acinetobacter , but few offer high resolution, rapid turnaround time, technical ease and high inter-laboratory reproducibility, which has h ered understanding of disease incidence, transmission patterns and ersity within this genus. Here, we further evaluated multilocus PCR electrospray ionization/mass spectrometry (PCR/ESI-MS), a method that is simple and robust, and provides both species characterization and strain-level resolution of Acinetobacter spp. on a single platform. We examined 125 Acinetobacter isolates from 21 hospitals, laboratories and medical centres spanning four counties in Arizona, USA, using PCR/ESI-MS. We compared PCR/ESI-MS with an in-house lified fragment length polymorphism (AFLP) genotyping scheme. PCR/ESI-MS demonstrated that Acinetobacter spp. from Arizonan hospitals had similar species and strain distributions to other US civilian hospitals. Furthermore, we showed that the PCR/ESI-MS and AFLP genotypes were highly congruent, with the former having the advantages of robust inter-laboratory reproducibility, rapid turnaround time and simple experimental set-up and data analysis. PCR/ESI-MS is an effective and high-throughput platform for strain typing of Acinetobacter baumannii and for identification of other Acinetobacter spp., including the emerging nosocomial pathogens Acinetobacter pittii and Acinetobacter nosocomialis .
Publisher: Public Library of Science (PLoS)
Date: 15-01-2010
Publisher: American Society for Microbiology
Date: 02-11-2016
Abstract: Whole-genome sequence (WGS) data are commonly used to design diagnostic targets for the identification of bacterial pathogens. To do this effectively, genomics databases must be comprehensive to identify the strict core genome that is specific to the target pathogen. As additional genomes are analyzed, the core genome size is reduced and there is erosion of the target-specific regions due to commonality with related species, potentially resulting in the identification of false positives and/or false negatives. IMPORTANCE A comparative analysis of 1,130 Burkholderia genomes identified unique markers for many named species, including the human pathogens B. pseudomallei and B. mallei . Due to core genome reduction and signature erosion, only 38 targets specific to B. pseudomallei /mallei were identified. By using only public genomes, a larger number of markers were identified, due to unders ling, and this larger number represents the potential for false positives. This analysis has implications for the design of diagnostics for other species where the genomic space of the target and/or closely related species is not well defined.
Publisher: American Society for Microbiology
Date: 06-2012
DOI: 10.1128/JCM.06737-11
Abstract: Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei . In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1- orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia -like fimbrial/ Burkholderia thailandensis -like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.
Location: United States of America
No related grants have been discovered for Rebecca Colman.