ORCID Profile
0000-0003-1166-9976
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Publisher: Wiley
Date: 09-09-2020
DOI: 10.1111/PCE.13649
Abstract: Drought is a major stress impacting forest ecosystems worldwide. We utilized quantitative trait loci (QTL) analysis to study the genetic basis of variation in (a) drought resistance and recovery and (b) candidate traits that may be associated with this variation in the forest tree Eucalyptus globulus. QTL analysis was performed using a large outcrossed F
Publisher: Springer Science and Business Media LLC
Date: 2007
Publisher: Springer Science and Business Media LLC
Date: 11-04-2016
Publisher: Wiley
Date: 25-03-2008
DOI: 10.1111/J.1469-8137.2008.02417.X
Abstract: * Formylated phloroglucinols (FPCs) are key defensive compounds that influence herbivory by mammals and arthropods in eucalypts. However, the genetic architecture underlying variation in their levels remains poorly understood. * Quantitative trait loci (QTL) analysis for the concentrations of two major FPCs, sideroxylonal A and macrocarpal G, was conducted using juvenile leaves from 112 clonally duplicated progenies from an outcross F2 of Eucalyptus globulus. * Two unlinked QTL were located for macrocarpal, while another unlinked QTL was located for sideroxylonal. The sideroxylonal QTL collocated with one for total sideroxylonal previously reported using adult Eucalyptus nitens foliage, providing independent validation in a different evolutionary lineage and a different ontogenetic stage. * Given the potential widespread occurrence of these QTL, their ontogenetic stability, and their impact on a range of dependent herbivores, it is possible that they have extended phenotypic effects in the Australian forest landscape.
Publisher: Scientific Societies
Date: 08-2018
DOI: 10.1094/PDIS-01-18-0003-RE
Abstract: Intumescence is a nonpathogenic physiological disorder characterized by leaf blistering. This disorder can affect growth and development in glasshouses and growth chambers and may be confused with pathogenic diseases. We used quantitative trait loci (QTL) analysis to examine the genetic basis of variation in intumescence severity in Eucalyptus globulus, and test for colocation with previously detected QTLs for pathogen susceptibility. QTL analysis used the phenotype means of open-pollinated (OP) families of an outcrossed F 2 mapping family (OP F 3 n = 300) of E. globulus and the linkage map constructed in the F 2 . We validate this phenotyping approach for QTL analysis by assessing a trait previously used for QTL discovery in the F 2 and showing the same major QTL was detected with the OP F 3 . For intumescence severity, five putative QTLs were detected across four linkage groups. Four of these did not colocate with previously reported QTLs for fungal pathogen susceptibility in Eucalyptus, suggesting the mechanisms underlying susceptibility to intumescence and to the two fungal pathogens are largely independent. This study demonstrates there is a genetic basis for variation in intumescence severity, reports the first QTL for intumescence severity in plants, and provides a robust framework for investigating the potential mechanisms involved.
Publisher: Springer Science and Business Media LLC
Date: 28-08-2019
Publisher: Springer Science and Business Media LLC
Date: 22-05-2017
Publisher: Springer Science and Business Media LLC
Date: 16-11-2011
Publisher: Oxford University Press (OUP)
Date: 2008
DOI: 10.1534/GENETICS.107.081414
Abstract: Foliar fungal pathogens from the genus Mycosphaerella affect eucalypts in natural forests and plantations worldwide. QTL analysis was conducted to dissect the genetic control of resistance in Eucalyptus globulus to a natural infection by Mycosphaerella leaf disease, using a clonally replicated outbred F2 family (112 genotypes) planted in a field trial. Two major QTL, with high LOD support (20.2 and 10.9) and high genomewide significance, explained a large proportion (52%) of the phenotypic variance in the severity of damage by Mycosphaerella cryptica, which may be indicative of oligogenic control. Both QTL were validated in a second F2 family and one was validated in a third F2 family. The mean values of different genotype classes at both major QTL argue for Mendelian inheritance with resistance dominant over susceptibility. There were strong correlations between the levels of Mycosphaerella damage in related genetic material planted in three widely separated locations in Tasmania. These findings together provide evidence that the genes controlling resistance to Mycosphaerella damage are stable in different genetic backgrounds and across different environments.
Publisher: Wiley
Date: 11-08-2016
DOI: 10.1111/NPH.13600
Abstract: Plant cuticular wax compounds perform functions that are essential for the survival of terrestrial plants. Despite their importance, the genetic control of these compounds is poorly understood outside of model taxa. Here we investigate the genetic basis of variation in cuticular compounds in Eucalyptus globulus using quantitative genetic and quantitative trait loci ( QTL ) analyses. Quantitative genetic analysis was conducted using 246 open‐pollinated progeny from 13 native sub‐races throughout the geographic range. QTL analysis was conducted using 112 clonally replicated progeny from an outcross F 2 population. Nine compounds exhibited significant genetic variation among sub‐races with three exhibiting signals of ersifying selection. Fifty‐two QTL were found with co‐location of QTL for related compounds commonly observed. Notable among these was the QTL for five wax esters, which co‐located with a gene from the KCS family, previously implicated in the biosynthesis of cuticular waxes in Arabidopsis. In combination, the QTL and quantitative genetic analyses suggest the variation and differentiation in cuticular wax compounds within E. globulus has a complex genetic origin. Sub‐races exhibited independent latitudinal and longitudinal differentiation in cuticular wax compounds, likely reflecting processes such as historic gene flow and ersifying selection acting upon genes that have erse functions in distinct biochemical pathways.
Publisher: Springer Science and Business Media LLC
Date: 06-03-2013
Publisher: Oxford University Press (OUP)
Date: 07-2014
Abstract: A change in the timing or rate of developmental events throughout ontogeny is referred to as heterochrony, and it is a major evolutionary process in plants and animals. We investigated the genetic basis for natural variation in the timing of vegetative phase change in the tree Eucalyptus globulus, which undergoes a dramatic change in vegetative morphology during the juvenile-to-adult transition. Quantitative trait loci analysis in an outcross F2 family derived from crosses between in iduals from a coastal population of E. globulus with precocious vegetative phase change and in iduals from populations in which vegetative phase change occurs several years later implicated the microRNA EglMIR156.5 as a potential contributor to this heterochronic difference. Additional evidence for the involvement of EglMIR156.5 was provided by its differential expression in trees with early and late phase change. Our findings suggest that changes in the expression of miR156 underlie natural variation in vegetative phase change in E. globulus, and may also explain interspecific differences in the timing of this developmental transition.
Publisher: Wiley
Date: 20-03-2013
DOI: 10.1111/NPH.12237
Abstract: Eucalypts are one of the most planted tree genera worldwide, and there is increasing interest in marker‐assisted selection for tree improvement. Implementation of marker‐assisted selection requires a knowledge of the stability of quantitative trait loci ( QTL s). This study aims to investigate the stability of QTL s for wood properties and growth across contrasting sites and multiple pedigrees of E ucalyptus globulus . Saturated linkage maps were constructed using 663 genotypes from four separate families, grown at three widely separated sites, and were employed to construct a consensus map. This map was used for QTL analysis of growth, wood density and wood chemical traits, including pulp yield. Ninety‐eight QTL s were identified across families and sites: 87 for wood properties and 11 for growth. These QTL s mapped to 38 discrete regions, some of which co‐located with candidate genes. Although 16% of QTL s were verified across different families, 24% of wood property QTL s and 38% of growth QTL s exhibited significant genotype‐by‐environment interaction. This study provides the most detailed assessment of the effect of environment and pedigree on QTL detection in the genus. Despite markedly different environments and pedigrees, many QTL s were stable, providing promising targets for the application of marker‐assisted selection.
Publisher: Elsevier BV
Date: 05-2016
Publisher: Springer Science and Business Media LLC
Date: 17-11-2011
Publisher: Springer Science and Business Media LLC
Date: 12-11-2022
DOI: 10.1007/S11295-022-01572-9
Abstract: To understand the potential of forests to adapt to wildfire, we studied the genetic architecture of fire-related structural, damage and recovery traits in a globally important Australian forest tree species, Eucalyptus globulus . Fourteen traits were evaluated in an outcrossed F 2 population in a field trial in Tasmania, Australia, which was burnt by a wildfire 14 years after planting. The trial also included open-pollinated families of the grandparental dwarf and tall ecotypes used to produce the F 2 population. We studied the phenotypic correlations within the F 2 population and performed quantitative trait loci (QTL) analyses using a linkage map comprised of 472 markers. Ecotype comparisons revealed that almost all traits were under genetic control, with trees of the dwarf ecotype significantly more damaged and mainly recovering from lignotubers, whereas tall ecotype trees mainly recovered from epicormic resprouts extending for a variable height up the stem. Within the F 2 , tree size was negatively correlated with fire damage and positively correlated with recovery. Genetic control of fire-related traits was confirmed by the detection of 38 QTL in the F 2 population. These QTL accounted for 4 to 43% of the phenotypic variation in these traits. Several QTL co-located and likely reflect pleiotropic effects. However, many independent QTL were detected, including QTL for crown consumption and trunk scorch, epicormic resprouting, resprout herbivory, and seedling establishment. The QTL detected argue that many genetically controlled mechanisms are responsible for variation in fire damage and recovery.
Publisher: Springer Science and Business Media LLC
Date: 2012
Publisher: Springer Science and Business Media LLC
Date: 10-05-2021
DOI: 10.1038/S42003-021-02009-0
Abstract: Corymbia citriodora is a member of the predominantly Southern Hemisphere Myrtaceae family, which includes the eucalypts ( Eucalyptus , Corymbia and Angophora ~800 species). Corymbia is grown for timber, pulp and paper, and essential oils in Australia, South Africa, Asia, and Brazil, maintaining a high-growth rate under marginal conditions due to drought, poor-quality soil, and biotic stresses. To dissect the genetic basis of these desirable traits, we sequenced and assembled the 408 Mb genome of Corymbia citriodora , anchored into eleven chromosomes. Comparative analysis with Eucalyptus grandis reveals high synteny, although the two erged approximately 60 million years ago and have different genome sizes (408 vs 641 Mb), with few large intra-chromosomal rearrangements. C. citriodora shares an ancient whole-genome duplication event with E. grandis but has undergone tandem gene family expansions related to terpene biosynthesis, innate pathogen resistance, and leaf wax formation, enabling their successful adaptation to biotic/abiotic stresses and arid conditions of the Australian continent.
Publisher: Springer Science and Business Media LLC
Date: 07-2009
Publisher: Springer Science and Business Media LLC
Date: 06-09-2014
Publisher: Springer Science and Business Media LLC
Date: 15-02-2017
Publisher: CSIRO Publishing
Date: 2001
DOI: 10.1071/BT00094
Abstract: The pattern of variability in chloroplast DNA (cpDNA) of Eucalyptus globulus Labill. (Myrtaceae) was studied using 270 s les from southern Australia. Forty variable sequence characters were found, defining 105 haplotypes. Haplotypes were assigned to three major cpDNA clades based on their phylogeny. The pattern of cpDNA variation did not conform to subspecies boundaries however, there was a strong geographic structure to the distribution of clades and haplotypes. One clade (JC) was geographically central and widespread and was found in 163 s les from Tasmania and continental Australia. Less-common clades occurred in more localised regions—southern (Js, 77 s les) and eastern (Jet, 12 s les). The distribution of the J et and Js clades coincides with hypothesised glacial refugia in Tasmania, suggesting limited seed-mediated dispersal since deglaciation.
Publisher: Wiley
Date: 07-09-2017
DOI: 10.1111/NPH.13316
Abstract: We examined genome‐wide patterns of DNA sequence ersity and ergence among six species of the important tree genus Eucalyptus and investigated their relationship with genomic architecture. Using c . 90 range‐wide in iduals of each Eucalyptus species ( E. grandis , E. urophylla , E. globulus , E. nitens , E. dunnii and E. camaldulensis ), genetic ersity and ergence were estimated from 2840 polymorphic ersity arrays technology markers covering the 11 chromosomes. Species differentiating markers ( SDM s) identified in each of 15 pairwise species comparisons, along with species ersity ( H HW ) and ergence ( F ST ), were projected onto the E. grandis reference genome. Across all species comparisons, SDM s totalled 1.1–5.3% of markers and were widely distributed throughout the genome. Marker ergence ( F ST and SDM s) and ersity differed among and within chromosomes. Patterns of ersity and ergence were broadly conserved across species and significantly associated with genomic features, including the proximity of markers to genes, the relative number of clusters of tandem duplications, and gene density within or among chromosomes. These results suggest that genomic architecture influences patterns of species ersity and ergence in the genus. This influence is evident across the six species, encompassing erse phylogenetic lineages, geography and ecology.
Publisher: Springer Science and Business Media LLC
Date: 2013
Publisher: Springer Science and Business Media LLC
Date: 10-03-2018
Publisher: Springer Science and Business Media LLC
Date: 09-08-2016
No related grants have been discovered for Jules Freeman.