ORCID Profile
0000-0001-6362-6607
Current Organisations
University of Jyväskylä
,
Te?ted Oy
,
Beijing Institute of Technology
,
Affiliated Hospital of Southwest Medical University
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Publications
SARSPLEX: Multiplex Serological ELISA with a Holistic Approach
Publisher: MDPI AG
Date: 22-11-2022
DOI: 10.3390/V14122593
Abstract: Currently, there are over 602 million severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) cases and 6.4 million COVID-19 disease-related deaths worldwide. With ambitious vaccine strategies, reliable and accurate serological testing is needed to monitor the dynamics of the novel coronavirus pandemic and community immunity. We set out to improve serological testing of the immune response against SARS-CoV-2. We hypothesize that by multiplexing the serological diagnostic test kit (SARSPLEX) and screening for three antibodies, an even more robust diagnostic can be developed. A total of 293 sera were analyzed for IgM, IgG, or IgA immune reactions to the subunit 1 spike glycoprotein and the nucleocapsid protein in a standardized ELISA platform. Testing IgM, IgG, and IgA demonstrated high positive and negative agreements compared to RT-PCR and serology reference tests. Comparison with the pre-2019-CoV (n = 102) s les highlighted the specificity of this test kit and indicated that no unspecific binding, even with the summer flu patients (n = 44), was detected. In addition, SARSPLEX demonstrated to be a valuable occupational surveillance tool used in a functional medicine facility. With increased and broader testing, SARSPLEX will be a valuable tool in monitoring immunity and aid in prioritizing access to the SARS-CoV-2 vaccine for high-risk patients.
Leptin accelerates the pathogenesis of heterotopic ossification in rat tendon tissues via mTORC1 signaling
Publisher: Wiley
Date: 05-06-2017
DOI: 10.1002/JCP.25955
Abstract: Leptin, an adipocyte‐derived cytokine associated with bone metabolism, is believed to play a critical role in the pathogenesis of heterotopic ossification (HO). The effect and underlying action mechanism of leptin were investigated on osteogenic differentiation of tendon‐derived stem cells (TDSCs) in vitro and the HO formation in rat tendons. Isolated rat TDSCs were treated with various concentrations of leptin in the presence or absence of mTORC1 signaling specific inhibitor rapamycin in vitro. A rat model with Achilles tenotomy was employed to evaluate the effect of leptin on HO formation together with or without rapamycin treatment. In vitro studies with TDSCs showed that leptin increased the expression of osteogenic biomarkers (alkaline phosphatase, runt‐related transcription factor 2, osterix, osteocalcin) and enhanced mineralization of TDSCs via activating the mTORC1 signal pathway (as indicated by phosphorylation of p70 ribosomal S6 kinase 1 and p70 ribosomal S6). However, mTORC1 signaling blockade with rapamycin treatment suppressed leptin‐induced osteogenic differentiation and mineralization. In vivo studies showed that leptin promoted HO formation in the Achilles tendon after tenotomy, and rapamycin treatment blocked leptin‐induced HO formation. In conclusion, leptin can promote TDSC osteogenic differentiation and heterotopic bone formation via mTORC1 signaling in both vitro and vivo model, which provides a new potential therapeutic target for HO prevention.
Hot deformation behavior and constitutive model of TC18 alloy during compression
Publisher: Springer Science and Business Media LLC
Date: 08-2014
Hot deformation behavior of TC18 titanium alloy
Publisher: National Library of Serbia
Date: 2013
DOI: 10.2298/TSCI1305523J
Abstract: Isothermal compression tests of TC18 titanium alloy at the deformation temperatures ranging from 25?C to 800?C and strain rate ranging from 10-4 to 10-2 s-1 were conducted by using a WDW-300 electronic universal testing machine. The hot deformation behavior of TC18 was characterized based on an analysis of the true stress-true strain curves of TC18 titanium alloy. The curves show that the flow stress increases with increasing the strain rate and decreases with increasing the temperature, and the strain rate play an important role in the flow stress when increasing the temperatures. By taking the effect of strain into account, an improved constitutive relationship was proposed based on the Arrhenius equation. By comparison with the experimental results, the model prediction agreed well with the experimental data, which demonstrated the established constitutive relationship was reliable and can be used to predict the hot deformation behavior of TC18 titanium alloy.
Related Organisations
University Hospital Carl Gustav Carus At Technology University Of Dresden
Location: Germany
Related Funding Activities
No related grants have been discovered for Weidong Song.