ORCID Profile
0000-0003-1722-5954
Current Organisation
Queensland Museum
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In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Medicinal and Biomolecular Chemistry | Evolutionary Biology | Oceanography | Proteins and Peptides | Biological Oceanography | Sensory Systems | Pharmaceutical Sciences And Pharmacy | Animal Systematics, Taxonomy And Phylogeny | Neurosciences | Marine And Estuarine Ecology (Incl. Marine Ichthyology) | Microbial Systematics, Taxonomy And Phylogeny | Geology Not Elsewhere Classified | Conservation And Biodiversity | Oceanography Not Elsewhere Classified | Bioinformatics | Animal Systematics and Taxonomy |
Biological sciences | Earth sciences | Expanding Knowledge in the Medical and Health Sciences | Physical sciences | Living resources (incl. impacts of fishing on non-target species) | Integrated (ecosystem) assessment and management | Expanding Knowledge in the Chemical Sciences | Expanding Knowledge in the Biological Sciences | Treatments (e.g. chemicals, antibiotics) | Other
Publisher: MDPI AG
Date: 12-02-2019
DOI: 10.3390/MD17020115
Abstract: Marine natural products (MNPs) continue to be in the spotlight in the global drug discovery endeavor. Currently, more than 30,000 structurally erse secondary metabolites from marine sources have been isolated, making MNPs a profound, renewable source to investigate novel drug compounds. Marine sponges of the genus Suberea (family: Aplysinellidae) are recognized as producers of bromotyrosine derivatives, which are considered distinct chemotaxonomic markers for the marine sponges belonging to the order Verongida. This class of compounds exhibits structural ersity, ranging from simple monomeric molecules to more complex molecular scaffolds, displaying a myriad of biological and pharmacological potentialities. In this review, a comprehensive literature survey covering the period of 1998–2018, focusing on the chemistry and biological harmacological activities of marine natural products from marine sponges of the genus Suberea, with special attention to the biogenesis of the different skeletons of halogenated compounds, is presented.
Publisher: Wiley
Date: 04-2000
DOI: 10.1046/J.1463-6409.2000.00029.X
Abstract: Alvarez, B., Crisp, M.D., Driver, F., Hooper, J.N.A. & Van Soest, R.W.M. (2000). Phylogenetic relationships of the family Axinellidae (Porifera: Demospongiae) using morphological and molecular data. — Zoologica Scripta , 29, 169–198. Twenty‐seven species of marine sponges belonging to Axinellidae and related groups (Halichondriidae, Dictyonellidae, Agelasida) were selected to test the monophyly of Axinellidae and investigate their phylogenetic relationships using parsimony and maximum likelihood methods. Partial 28S rDNA sequences, including the D3 domain, and traditional morphological characters (mainly skeletal ones) were used independently to construct phylogenetic trees. Sequences were aligned using the appropriate model of secondary structure of the RNA and compared to that produced by the multiple sequence alignment program, ClustalW. The alignment using secondary structure constraints produced a better estimate of the phylogeny and was demonstrated to be an effective and objective method. Results of the cladistic analyses of the molecular and morphological data sets were not fully congruent the morphological data suggest that Axinellidae is monophyletic, however, the molecular data suggest that it is nonmonophyletic. The single most‐parsimonious tree derived from the molecular data showed that species of Axinella (except A. polypoides ) are united in a clade that is more closely related to members of Agelasida than to other species of Axinellidae the remaining members of Axinellidae form a monophyletic group that is closely related to the families Dictyonellidae and Halichondriidae. The consensus tree of 20 most‐parsimonious trees from the morphological analysis, on the other hand, showed that all the s led species of Axinellidae belong to a monophyletic group which is closely related to the species of Dictyonellidae and Halichondriidae. Only two branches were identical in both cladograms, the one uniting the species of Ptilocaulis and Reniochalina and the one with the species of Dictyonellidae. The robustness of the molecular and morphological trees (or parts of the trees), was tested using bootstrap, jack‐knife, PTP and T‐PTP tests. The results of the PTP test were significant indicating significant cladistic structure in both data sets. The bootstrap and jack‐knife values indicate that the molecular tree is in general better supported than the morphological one. The lack of morphological characters and the homoplastic nature of some may explain the weak support of the morphological tree. A T‐PTP test of nonmonophyly showed that the nonmonophyly of Axinellidae, as indicated by the results of the molecular analysis, is not significant however, a T‐PTP test of monophyly of Axinellidae, as indicated by the morphological tree, produced significant results. This indicates that the monophyly of Axinellidae based on morphological data cannot be rejected the family however, cannot be defined in terms of a unique diagnostic character common to all members of the ingroup. Tests of heterogeneity (reciprocal T‐PTP and partition homogeneity test) indicated that the data partitions are heterogeneous, which could be due to s ling errors (in either data set) or differences in the underlying phylogenies therefore data were not combined in a single analysis. Further, both data sets are unequally sized (95 informative molecular characters vs. 16 informative morphological characters), which means that the molecular signal could sw the morphological signal if the data is combined. Nonmonophyly of Axinellidae is supported by chemical and genetic evidence available in the literature and DNA sequences data of axinellid species from New Zealand. However, this needs to be confirmed using independent evidence from different genes (or gene regions), biochemistry, histology or cell ultrastructure. Therefore, no changes to the taxonomic position of the family in the higher classification are proposed at this stage.
Publisher: Cambridge University Press (CUP)
Date: 12-2007
DOI: 10.1017/S0025315407058201
Abstract: We present a 28S rDNA gene tree of selected Raspailiidae, Axinellidae and other demosponges to obtain insight into raspailiid phylogeny and character evolution. The Raspailiidae in our data set cluster in a well-supported clade, distinguished from Axinellidae, Agelasida and Hadromerida. Raspailia ( s . s .), Eurypon , Sollasella , Aulospongus and Ectyoplasia form a Raspailiidae clade. Some Raspailia subgenera, in particular R . ( Parasyringella ), are not retrieved monophyletically. Trikentrion falls into the Thrinacophorinae, and not the Cyamoninae as earlier hypothesized. The axinellid genera Ptilocaulis and Reniochalina also cluster with Raspailiidae, distant from the other Axinellidae. The suitability of particular morphological characters for raspailiid phylogeny is discussed.
Publisher: Cambridge University Press (CUP)
Date: 22-06-2016
DOI: 10.1017/S0025315415000831
Abstract: Three species of lithistid sponges, Neoaulaxinia zingiberadix, Isabella mirabilis and Neoschrammeniella fulvodesmus were collected from deep seamounts off New Caledonia to address questions about their population structure, gene flow and the relative contribution of sexual and asexual reproductive strategies to their populations. The sponges were tested by sequencing the ITS (internal transcribed spacer) and CO1 regions of their genomes. These rare and presumably ancient sponges have a distribution restricted to seamounts in the south-western Pacific. Deep seamounts represent geographically separated islands. Although the sponges could be expected to have sexual reproduction restricted to near neighbours due to low sexual dispersal opportunities via larvae, this study found surprisingly high levels of gene flow between the seamounts. Amongst the specimens of N. zingiberadix taken from two seamounts there was no population structure CO1 resulted in identical genotypes. For the population structure within N. fulvodesmus , as revealed by ITS, most of the variation was within each in idual from the six seamounts on which it occurred and CO1 revealed no difference between in iduals or seamounts. The third species I. mirabilis showed four genotypes based on CO1, which were distributed across all the seamounts. Indirect measures of different species showed a range of reproductive strategies from asexual to sexual, but with much higher connection between seamounts than previously thought. In idual seamounts did not show a separate population structure as one might expect from ‘islands’. The conclusion must be that these sponges have mechanisms to attain greater dispersal than previously thought.
Publisher: American Chemical Society (ACS)
Date: 26-01-2002
DOI: 10.1021/NP0104020
Abstract: Two new isomalabaricane triterpenes, stellettin H (1) and stellettin I (2), have been isolated from the marine sponge Rhabdastrella globostellata, collected from the Philippines. Stellettins A-D (3-6), (-)-stellettin E (7), and rhabdastrellic acid-A (8) were also isolated and characterized. Stellettin B (4) and (-)-stellettin E (7) showed selective cytotoxicity toward p21(WAF1/Cip1)-deficient human colon tumor (HCT-116) cells with IC(50) values of 0.043 and 0.039 microM, respectively.
Publisher: Springer Science and Business Media LLC
Date: 18-11-2012
DOI: 10.1007/S10482-011-9676-9
Abstract: Isolates from the marine actinobacterial genus Salinispora were cultured from marine sponges collected from along the length of the Great Barrier Reef (GBR), Queensland, Australia. Strains of two species of Salinispora, Salinispora arenicola and "Salinispora pacifica", were isolated from GBR sponges Dercitus xanthus, Cinachyrella australiensis and Hyattella intestinalis. Phylogenetic analysis of the 16S rRNA gene sequences of representative strains, selected via BOX-PCR screening, identified previously unreported phylotypes of the species "S. pacifica". The classification of these micro erse 16S rRNA groups was further confirmed by analysis of the ribonuclease P RNA (RNase P RNA) gene through both phylogenetic and secondary structure analysis. The use of RNase P RNA sequences combined with 16S rRNA sequences allowed distinction of six new intraspecies phylotypes of "S. pacifica" within the geographical area of the GBR alone. One of these new phylotypes possessed a localised regional distribution within the GBR.
Publisher: American Chemical Society (ACS)
Date: 20-01-2022
DOI: 10.1021/ACS.JNATPROD.1C01172
Abstract: Seven new polyaromatic bis-spiroketal-containing butenolides, the prunolides D-I (
Publisher: American Chemical Society (ACS)
Date: 14-02-2013
DOI: 10.1021/NP300828Y
Publisher: Wiley
Date: 06-2006
Publisher: Queensland Museum
Date: 2021
DOI: 10.17082/J.2204-1478.62.2021.2020-06
Abstract: A new species of carnivorous sponge, Lycopodina coralseaensis sp. nov., family Cladorhizidae, is described from the Louisiade Plateau, Coral Sea, off the east coast of Australia at bathyal depths of ~2000 m, collected during the RV Investigator 2019 voyage. The new species differs from other Lycopodina with stipitate morphology by lacking forceps microscleres, similar to L. nikitawimandi Ekins et al., 2020a but differing in having only a very small single size class of palmate anisochelae, and most importantly by having a third category of mycalostyle echinating the stem and basal holdfast. This new species is the sixth Lycopodina recently described as new from the Australian Exclusive Economic Zone (L. nikitawimandi, L. helios, L. cassida, L. brochidodroma and L. hystrix), bringing the total number of carnivorous sponges known so far from Australia to 26.
A new species of lithistid sponge hiding within the Isabella mirabilis species complex (Porifera: Demospongiae: Tetractinellida) from seamounts of the Norfolk Ridge
Publisher: Magnolia Press
Date: 07-07-2016
DOI: 10.11646/ZOOTAXA.4136.3.2
Abstract: A population level study of the lithistid ('rock') sponge, Isabella mirabilis, revealed a new species, Isabella tanoa sp. nov., living on five seamounts on the Norfolk Ridge, SW Pacific, and representing the third species to be discovered since the genus was first described in 2005. Comparisons between the three species showed significant differences in morphological characters that corresponded to differences in their respective CO1 barcoding sequences. Conversely, three of the four genotypes of Isabella mirabilis remain unresolved using morphological markers.
Publisher: American Chemical Society (ACS)
Date: 16-11-2001
DOI: 10.1021/NP010246X
Abstract: Three new amino alcohols presumably deriving from L-alanine were isolated from the tropical marine sponge Haliclona n. sp. and characterized by 2D NMR, while a fourth amino alcohol was characterized as an acetamide derivative. Relative stereochemistry was deduced from the NMR characteristics of oxazolidinone derivatives and absolute stereochemistry secured by preparation and analysis of an MPA ester. The amino alcohol fraction from Haliclona n. sp. acts as an antifungal agent and inhibits the development of larvae of the ascidian Herdmania curvata.
Publisher: Magnolia Press
Date: 06-07-2016
DOI: 10.11646/ZOOTAXA.4136.2.11
Abstract: The use of nuclear markers, in addition to traditional mitochondrial markers, helps to clarify hidden patterns of genetic structure in natural populations (Palumbi & Baker, 1994). This is particularly evident among demosponges that possess slow mitochondrial evolutionary rates compared to Bilateria, where nuclear intron markers can aid in the understanding of shallow level phylogenetic relationships (Shearer et al., 2002). Ideally, these nuclear markers (i) are evolutionary well-conserved across different lineages, (ii) produce licons holding a number of sites with sufficient variability to answer the relevant phylogenetic question, (iii) derive from single copy genes (see review in Zhang & Hewitt, 2003). A popular method to lify intron markers uses EPIC (Exon-Primed, Intron-Crossing) primers that anneal to the more conserved flanking exon regions and subsequently bridge the intron during lification (Palumbi & Baker, 1994).
Publisher: CSIRO Publishing
Date: 2002
DOI: 10.1071/IT00036
Publisher: Springer Science and Business Media LLC
Date: 13-11-2015
DOI: 10.1007/S00239-014-9653-9
Abstract: We discovered for the first time a mitochondrial intron in a non-tetillid demosponge, which sheds new light on the interpretation of mitochondrial intron evolution among non-bilaterian animals and has consequences for phylogenetic and DNA barcoding studies. The newly discovered class 1 intron of Aplysinella rhax (Verongida) CO1 has an ORF for a putative LAGLIDADG-type and resembles other sponge and cnidarian mitochondrial introns. Our analysis of the Aplysinella rhax intron underlines that the patchy distribution of introns in sponges is caused by a combination of horizontal and vertical transmission. Further implications for CO1 phylogenetic and barcoding projects are discussed.
Publisher: Cambridge University Press (CUP)
Date: 12-02-2016
Publisher: Elsevier BV
Date: 08-2014
DOI: 10.1016/J.BMCL.2014.05.104
Abstract: Mass-guided fractionation of the MeOH extract from a specimen of the Australian marine sponge Hyrtios sp. resulted in the isolation of two new tryptophan alkaloids, 6-oxofascaplysin (2), and secofascaplysic acid (3), in addition to the known metabolites fascaplysin (1) and reticulatate (4). The structures of all molecules were determined following NMR and MS data analysis. Structural ambiguities in 2 were addressed through comparison of experimental and DFT-generated theoretical NMR spectral values. Compounds 1-4 were evaluated for their cytotoxicity against a prostate cancer cell line (LNCaP) and were shown to display IC50 values ranging from 0.54 to 44.9 μM.
Publisher: Public Library of Science (PLoS)
Date: 04-2010
Publisher: American Chemical Society (ACS)
Date: 11-2007
DOI: 10.1021/NP0703646
Abstract: The sponge Psammoclemma sp. was investigated as part of our studies to discover P2X 7 receptor antagonists for the treatment of inflammatory disease. The biological activity of this extract was found to be due to the cytotoxicity of two new bromotyrosine alkaloids, psammaplysenes C (1) and D (2), and not P2X 7-specific activity. Their structures were determined by 1D and 2D NMR spectroscopy.
Publisher: MDPI AG
Date: 27-12-2017
DOI: 10.3390/MD15010006
Publisher: Elsevier BV
Date: 03-2005
DOI: 10.1016/J.CHEMBIOL.2005.02.003
Abstract: Four species of marine sponges (Phylum Porifera, Order Dictyoceratida), which contain the filamentous cyanobacterial symbiont Oscillatoria spongeliae, were collected from four locations in Palau. The halogenated natural products associated with the symbiont were characterized from each s le, revealing that each species contained either chlorinated peptides, brominated diphenyl ethers, or no halogenated compounds. Analysis of the host sponges and the symbionts indicated that each species of sponge contained a distinct strain of morphologically similar cyanobacteria. Although cospeciation may be present in this group, we have identified that at least one host switching event has occurred in this symbiosis. Only the strain of O. spongeliae in the sponge containing the chlorinated compounds possessed genes involved in the biosynthesis of chlorinated leucine precursors, indicating that the chemical variation observed in these animals has a genetic foundation.
Publisher: MDPI AG
Date: 07-02-2021
DOI: 10.3390/MD19020095
Abstract: Two new fluorescent pteridine alkaloids, tedaniophorbasins A (1) and B (2), together with the known alkaloid N-methyltryptamine, were isolated, through application of mass directed purification, from the sponge Tedaniophorbas ceratosis collected from northern New South Wales, Australia. The structures of tedaniophorbasins A and B were deduced from the analysis of 1D/2D NMR and MS data and through application of 13C NMR DFT calculations. Tedaniophorbasin A possesses a novel 2-imino-1,3-dimethyl-2,3,7,8-tetrahydro-1H-[1,4]thiazino[3,2-g]pteridin-4(6H)-one skeleton, while tedaniophorbasin B is its 2-oxo derivative. The compounds show significant Stokes shifts (~14,000 cm−1) between excitation and emission wavelengths in their fluorescence spectra. The new compounds were tested for bioactivity against chloroquine-sensitive and chloroquine-resistant strains of the malaria parasite Plasmodium falciparum, breast and pancreatic cancer cell lines, and the protozoan parasite Trypanosoma brucei brucei but were inactive against all targets at 40 µM.
Publisher: American Chemical Society (ACS)
Date: 12-04-2008
DOI: 10.1021/NP070658R
Abstract: High-throughput screening of a plant and marine invertebrate extract library to find natural products with rat thyrotropin releasing hormorne (TRH) receptor 2 binding affinity led to the isolation of four new (1-4) and one known (5) spongian diterpene from the sponge Spongia sp. The structures were assigned from interpretation of 2D NMR and high-resolution ESIMS data. The absolute configurations of 1-4 were proposed on the basis of analysis of their CD spectra. Diterpenes 1-5 showed rat TRH receptor 2 binding affinity with IC(50) values of 23 microM, 70 microM, 400 microM, 600 microM, and 1 mM, respectively.
Publisher: American Chemical Society (ACS)
Date: 30-01-2004
DOI: 10.1021/JM030207D
Abstract: The Indo-Pacific marine sponge Ircinia ramosa has been found to contain two powerful (GI50 from 0.001 to <0.0001 microg/mL) murine and human cancer cell growth inhibitors. Both were isolated (10(-3)-10(-4)% yields) by cancer cell line bioassay-guided techniques and named irciniastatins A (1) and B (2). Structural elucidation by a combination of spectral analyses, primarily high resolution mass and 2D-NMR (principally APT, HMQC, HMBC, and ROESY) spectroscopy, revealed the unusual structures 1 and 2.
Publisher: Elsevier BV
Date: 12-2005
DOI: 10.1016/J.TOXICON.2005.08.009
Abstract: Contact with sponges (Phylum Porifera) usually results in minimal effects or abrasions, except for species that produce crinitoxins and can cause irritation and dermatitis. There are few reports of sponge stings, mainly in ers or collectors. We report a group of sponge stings from handling flame red/orange sponges on the beach, confirmed to be Tedania anhelans in five cases. All seven patients suffered immediate effects ranging from mild to severe pain, and local inflammation. A 38-year-old female and three children had delayed skin involvement including itchiness, pain, swelling and redness. Blistering and desquamation occurred in the female adult and limited desquamation in one child. Similar delayed effects have been reported in Tedania spp. stings previously.
Publisher: American Chemical Society (ACS)
Date: 03-2001
DOI: 10.1021/NP000546Z
Abstract: A new purine derivative microxine (1) was isolated from the Australian marine sponge Microxina sp. The compound was isolated via reversed-phase chromatography and its structure determined spectroscopically. Microxine was found to weakly inhibit cdc2 kinase activity with an IC(50) of 13 microM.
Publisher: American Chemical Society (ACS)
Date: 20-05-2008
DOI: 10.1021/OL800898Z
Abstract: Bioassay-guided fractionation of the methanol extract of the Australian sponge Neopetrosia exigua led to the isolation of exiguaquinol (2), a new pentacyclic hydroquinone that inhibited Helicobacter pylori glutamate racemase (MurI) with an IC(50) of 4.4 microM. Its structure and relative configuration were assigned on the basis of spectroscopic data. Exiguaquinol (2), bearing a novel pentacyclic ring skeleton, is the first natural product to show inhibition of H. pylori MurI. Its protein-ligand modeling is also discussed.
Publisher: Magnolia Press
Date: 17-04-2018
DOI: 10.11646/ZOOTAXA.4410.2.7
Abstract: A new species of Raspailia (Raspaxilla) frondosa sp. nov. is described from the deep seamounts of the Norfolk and New Caledonia Ridges. The morphology of the species resembles that of a frond or a fern, and its unique highly compressed axial skeleton of interlaced spongin fibres without spicules in combination with a radial extra axial skeleton of a perpendicular palisade of spicules, differentiate it from all other species of the subgenus. This species is compared morphologically to all 18 other valid species described in Raspailia (Raspaxilla).
Publisher: Wiley
Date: 09-2002
DOI: 10.1046/J.1365-294X.2002.01570.X
Abstract: Leucetta 'chagosensis' is a widespread calcareous sponge, occurring in shaded habitats of Indo-Pacific coral reefs. In this study we explore relationships among 19 ribosomal DNA sequence types (the ITS1-5.8S-ITS2 region plus flanking gene sequences) found among 54 in iduals from 28 locations throughout the western Pacific, with focus on the Great Barrier Reef (GBR). Maximum parsimony analysis revealed phylogeographical structuring into four major clades (although not highly supported by bootstrap analysis) corresponding to the northern/central GBR with Guam and Taiwan, the southern GBR and subtropical regions south to Brisbane, Vanuatu and Indonesia. Subsequent nested clade analysis (NCA) confirmed this structure with a probability of > 95%. After NCA of geographical distances, a pattern of range expansion from the internal Indonesian clade was inferred at the total cladogram level, as the Indonesian clade was found to be the internal and therefore oldest clade. Two distinct clades were found on the GBR, which narrowly overlap geographically in a line approximately from the Whitsunday Islands to the northern Swain Reefs. At various clade levels, NCA inferred that the northern GBR clade was influenced by past fragmentation and contiguous range expansion events, presumably during/after sea level low stands in the Pleistocene, after which the northern GBR might have been recolonized from the Queensland Plateau in the Coral Sea. The southern GBR clade is most closely related to subtropical L. 'chagosensis', and we infer that the southern GBR probably was recolonized from there after sea level low stands, based on our NCA results and supported by oceanographic data. Our results have important implications for conservation and management of the GBR, as they highlight the importance of marginal transition zones in the generation and maintenance of species rich zones, such as the Great Barrier Reef World Heritage Area.
Publisher: Elsevier BV
Date: 02-2007
Publisher: American Chemical Society (ACS)
Date: 20-04-2000
DOI: 10.1021/NP990431Z
Abstract: The new cytotoxic compounds, mycalamides C (3) and D (4), have been isolated from the marine sponge Stylinos n. sp., along with the known theopederin E (1) and mycalamide A (2).
Publisher: Oxford University Press (OUP)
Date: 09-2008
Abstract: A Polycomb-Group (PcG) complex, FERTILIZATION INDEPENDENT SEED (FIS), represses endosperm development in Arabidopsis thaliana until fertilization occurs. The Hieracium genus contains apomictic species that form viable seeds asexually. To investigate FIS function during apomictic seed formation, FERTILIZATION INDEPENDENT ENDOSPERM (FIE), encoding a WD-repeat member of the FIS complex, was isolated and downregulated in sexual and apomictic Hieracium species. General downregulation led to defects in leaf and seed development, consistent with a role in developmental transitions and cell fate. PcG-like activity of Hieracium FIE was also supported by its interaction in vitro with the Arabidopsis CURLY LEAF PcG protein. By contrast, specific downregulation of FIE in developing seeds of sexual Hieracium did not result in autonomous endosperm proliferation but led to seed abortion after cross-pollination. Furthermore, in apomictic Hieracium, specific FIE downregulation inhibited autonomous embryo and endosperm initiation, and most autonomous seeds displayed defective embryo and endosperm growth. Therefore, FIE is required for both apomictic and fertilization-induced seed initiation in Hieracium. Since Hieracium FIE failed to interact with FIS class proteins in vitro, its partner proteins might differ from those in the FIS complex of Arabidopsis. These differences in protein interaction were attributed to structural modifications predicted from comparisons of Arabidopsis and Hieracium FIE molecular models.
Publisher: Wiley
Date: 17-08-2012
Publisher: MDPI AG
Date: 18-06-2018
DOI: 10.3390/MD16060214
Publisher: Elsevier BV
Date: 08-2014
DOI: 10.1016/J.BMCL.2014.05.054
Abstract: High throughput screening of a pre-fractionated natural product library identified 11 active fractions showing ApoE modulation activity. Mass-directed fractionation of one active crude extract from the Australian marine sponge Callyspongia sp. resulted in the isolation of 13 metabolites, including three new bromotyrosine derivatives, callyspongic acid (1), 3,5-dibromo-4-methoxyphenylpyruvic acid (2), N-acetyl-3-bromo-4-hydroxylphenylethamine (3), and ten known compounds (4-13). The structure elucidation of compounds 1-3 was based on their 1D and 2D NMR and MS spectroscopic data. 3,5-Dibromo-4-methoxyphenylpyruvic acid (2) showed weak activity in increasing the apolipoprotein E secretion from human CCF-STTG1 cells at the concentration of 40 μM.
Publisher: Pacific Science
Date: 10-2013
DOI: 10.2984/67.4.1
Publisher: Public Library of Science (PLoS)
Date: 27-04-2012
Publisher: American Chemical Society (ACS)
Date: 05-04-2008
DOI: 10.1021/NP0706623
Abstract: The anticancer target isoprenylcysteine carboxyl methyltransferase (Icmt) was the focus of a natural product high-throughput screening c aign. The Australian marine sponge Pseudoceratina sp. yielded aplysamine 6, a new bromotyrosine derivative with an alpha,beta-unsaturated amide linkage, as the bioactive constituent. Its structure was determined by 1D and 2D NMR spectroscopy.
Publisher: MDPI AG
Date: 02-01-2018
DOI: 10.3390/NU10010033
Publisher: American Chemical Society (ACS)
Date: 11-2006
DOI: 10.1021/NP060244I
Abstract: Five new norsesterterpenes, mooloolabenes A-E (1-5), and the new sesterterpene mooloolaldehyde (6), related to the scalarane family of compounds, were isolated from an acetone extract of the Australian sponge Hyattella intestinalis. Structural elucidation, including relative stereochemical assignment, was based on spectroscopic analysis. All compounds tested showed cytotoxic activity against the P388 cell line.
Publisher: Elsevier BV
Date: 09-2015
Publisher: American Chemical Society (ACS)
Date: 14-01-1999
DOI: 10.1021/JO981034G
Abstract: Four imidazo-azolo-imidazole alkaloids, axinellamines A-D, have been isolated from an Australian marine sponge, Axinella sp. (order: Halichondrida: family: Axinellidae). These compounds contain a unique perhydrocyclopenta-imidazo-azolo-imidazole carbon skeleton. Three of these compounds had bactericidal activity against Helicobacter pylori at 1000 &mgr M.
Publisher: Elsevier BV
Date: 07-1996
Publisher: American Chemical Society (ACS)
Date: 14-03-2013
DOI: 10.1021/NP300648D
Abstract: Two new bromotyrosine alkaloids, pseudoceralidinone A (1) and aplysamine 7 (2), along with three known compounds were isolated from the Australian sponge Pseudoceratina verrucosa. Their structures were characterized by NMR and MS data and the synthetic route. Their cytotoxicity was evaluated against cancer cell lines (HeLa and PC3) and a noncancer cell line (NFF).
Publisher: Magnolia Press
Date: 20-07-2023
DOI: 10.11646/ZOOTAXA.5318.3.4
Abstract: Four new species of encrusting Hamacantha (Vomerula) are described from bathyal depths of seamounts off Queensland and Tasmania in southeast Australia (H. (V.) novacula sp. nov., H. (V.) melliflura sp. nov., H. (V.) ridleyi sp. nov., H. (V.) levii sp. nov.). A previously described species known only from New Caledonia (H. (V.) acerata Lévi, 1993) has also been redescribed. A tabular comparison of all known species of H. (Vomerula) is provided, bringing the number of known species in the subgenus to 26. This is also the first record of the genus Hamacantha from the Australian Exclusive Economic Zone.
Publisher: American Chemical Society (ACS)
Date: 31-01-2011
DOI: 10.1021/NP100613P
Publisher: Oxford University Press (OUP)
Date: 07-05-2013
DOI: 10.1093/ICB/ICT038
Abstract: Contemporary collections of sponges in the Indo-west Pacific have escalated substantially due to pharmaceutical discovery, national bioregional planning, and compliance with international conventions on the seabed and its marine genetic resources beyond national jurisdictions. These partially processed operational taxonomic unit (OTU) collections now vastly outweigh the expertise available to make them better "known" via complete taxonomy, yet for many bioregions they represent the most significant body of currently available knowledge. Increasing numbers of cryptic species, previously undetected morphologically, are now being discovered by molecular and chemical analyses. The uncoordinated and fragmented nature of many previous collections, however, means that knowledge and expertise gained from a particular project are often lost to future projects without a bio ersity informatics legacy. Integrating these erse data (GIS OTUs images molecular, chemical, and other datasets) required a two-way iterative process so far unavailable for sponges with existing bio ersity informatics tools. SpongeMaps arose from the initial need for online collaboration to integrate morphometric data with molecular barcodes, including the Porifera Tree of Life (PorTol) project. It provides interrogation of existing data to better process new collections capacity to create new OTUs publication of online pages for in idual species, so as to interpret GIS and other data for online bio ersity databases and services and automatic links to external datasets for taxonomic hierarchy, specimen GIS and mapping, DNA sequence data, chemical structures, and images.
Publisher: Cold Spring Harbor Laboratory
Date: 12-2008
DOI: 10.1101/PDB.EMO108
Abstract: Sponges are one of the earliest branching metazoans. In addition to undergoing complex development and differentiation, they can regenerate via stem cells and can discern self from nonself (“allorecognition”), making them a useful comparative model for a range of metazoan-specific processes. Molecular analyses of these processes have the potential to reveal ancient homologies shared among all living animals and critical genomic innovations that underpin metazoan multicellularity. Amphimedon queenslandica (Porifera, Demospongiae, Haplosclerida, Niphatidae) is the first poriferan representative to have its genome sequenced, assembled, and annotated. Amphimedon exemplifies many sessile and sedentary marine invertebrates (e.g., corals, ascidians, bryozoans): They disperse during a planktonic larval phase, settle in the vicinity of conspecifics, ward off potential competitors (including incompatible genotypes), and ensure that brooded eggs are fertilized by conspecific sperm. Using genomic and expressed sequence tag (EST) resources from Amphimedon , functional genomic approaches can be applied to a wide range of ecological and population genetic processes, including fertilization, dispersal, and colonization dynamics, host-symbiont interactions, and secondary metabolite production. Unlike most other sponges, Amphimedon produce hundreds of asynchronously developing embryos and larvae year-round in distinct, easily accessible brood chambers. Embryogenesis gives rise to larvae with at least a dozen cell types that are segregated into three layers and patterned along the body axis. In this article, we describe some of the methods currently available for studying A. queenslandica , focusing on the analysis of embryos, larvae, and post-larvae.
Publisher: Informa UK Limited
Date: 20-09-2008
DOI: 10.1080/14786410701763411
Abstract: The new compound ianthesine E (1) was isolated from the Great Barrier Reef marine sponge Pseudoceratina sp. along with the known metabolites 11-hydroxyaerothionin (2), aerothionin (3) and 11,19-dideoxyfistularin 3 (4). Structures were determined on the basis of their spectroscopic data. The compounds were tested for inhibition of [(3)H] DPCPX binding to adenosine A(1) receptors in a whole cell binding assay. At 100 microM, aerothionin was the most potent, inhibiting 67% of binding, followed by ianthesine E and 11-hydroxyaerothionin which inhibited 61% of binding, and 11,19-dideoxyfistularin which initiated 51% of binding Ianthesine E (EC(50) 60 microM), aerothionin (EC(50) 42 microM) and 11,19-dideoxyfistularin-3 (EC(50) 2.6 microM) exhibited moderate cytotoxicity against the HeLa cell line.
Publisher: American Chemical Society (ACS)
Date: 11-1997
DOI: 10.1021/NP970287M
Publisher: Oxford University Press (OUP)
Date: 18-08-2010
Abstract: The transposition of parts of the mitochondrial (mt) genetic material into the nuclear genome (NUMTs) occurs in a wide range of eukaryotes. Here, we show that NUMTs exist for nearly all regions of the mt genome in the demosponge Amphimedon queenslandica, a representative of the oldest phyletic lineage of animals. Because the sponge NUMTs are small and noncoding, and transposed via a DNA intermediate, as in eumetazoans, we infer that the transpositonal processes underlying NUMT formation in contemporary animals existed in their most recent common ancestor. In contrast to most bilaterians, Amphimedon NUMTs are inserted into regions of high gene density. Given the common features of metazoan NUMTs, the reduction in animal mt genome sizes relative to other eukaryotes may be the product of the mt DNA transposition mechanisms that evolved along the metazoan stem.
Publisher: Elsevier BV
Date: 04-2011
Publisher: American Chemical Society (ACS)
Date: 28-04-2001
DOI: 10.1021/NP000637W
Publisher: American Chemical Society (ACS)
Date: 17-10-2002
DOI: 10.1021/JA020814A
Abstract: A new marine natural product dysinosin A 1 has been isolated from a new genus and species of sponge of the family Dysideidae found near Lizard Island, North Queensland, Australia. Dysinosin A is a potent inhibitor of the blood coagulation cascade factor VIIa and an inhibitor of the serine protease thrombin. Among the distinctive features of dysinosin A are the presence of a 5,6-dihydroxy-octahydroindole-2-carboxylic acid, 3-amino-ethyl 1-N-amidino-Delta-3-pyrroline, a sulfated glyceric acid, and d-leucine, assembled through three peptidic linkages. Dysinosin A inhibited factor VIIa at a Ki of 108 nM and thrombin at a Ki of 452 nM. The identification of the 1-N-amidino-Delta-3-pyrroline and 5,6-dihydroxy-octahydroindole-2-carboxylic acid as P1 and P2 moieties respectively, should pave the way for the design and synthesis of new structure-based inhibitors.
Publisher: American Chemical Society (ACS)
Date: 20-04-2002
DOI: 10.1021/NP010581L
Abstract: Two new pyrroloquinoline alkaloids, isobatzelline E (1) and batzelline D (2), together with the known compounds batzelline C (3), isobatzelline C (4), and makaluvamine D (5), were isolated from an Indopacific collection of the marine sponge Zyzzya fuliginosa the known compounds makaluvamines A (9), H (10), J (7), K (8), and P (6) were obtained from Z. fuliginosa collected in Papua New Guinea. The structures were elucidated by interpretation of 1D (1)H and (13)C NMR spectra and 2D HSQC and HSQC-LR spectra. Compounds 1-10 were isolated because the crude extracts of both Zyzzya species inhibited HIV-1 envelope-mediated cell fusion. However, the inhibition profile observed for the pure compounds 1-10 mirrors that reported for the inhibition of topoisomerase II by other pyrroloquinolines, leaving open the possibility that the activity results from interactions with DNA-modifying enzymes.
Publisher: Magnolia Press
Date: 19-09-2023
Publisher: American Chemical Society (ACS)
Date: 13-03-2002
DOI: 10.1021/NP010347V
Abstract: 1,2-Bis(1H-indol-3-yl)ethane-1,2-dione (1), a bisindole alkaloid, was isolated from the marine sponge Smenospongia sp. The known compounds indole-3-carbaldehyde (2), 6-bromoindole-3-carbaldehyde (3), and tryptamine (4) and mixtures of the (Z/E) isomers of 6-bromo-2'-demethylaplysinopsin (5a/5b) and 6-bromo-3'-deimino-2',4'-bis(demethyl)-3'-oxoaplysinopsin (6a/6b) were also isolated.
Publisher: American Chemical Society (ACS)
Date: 11-09-1999
DOI: 10.1021/NP9901635
Abstract: The CH(2)Cl(2) extract of the marine sponge Xestospongia testudinaria inhibited [(3)H]DPCPX binding to rat-brain adenosine A(1) receptors. Bioassay-guided fractionation led to the isolation of a known brominated acetylenic fatty acid 1 as the active component. Also isolated were two novel sterol esters 2 and 3. All structures were determined on the basis of their spectroscopic data.
Publisher: MDPI AG
Date: 25-10-2016
DOI: 10.3390/D8040021
Publisher: American Chemical Society (ACS)
Date: 06-12-2012
DOI: 10.1021/NP3006474
Abstract: Three new cyclic depsipeptides, ne hamides B (2), C (3), and D (4), were isolated from the Australian sponge Ne hius huxleyi. The planar structural characterization of these molecules was elucidated using 2D NMR experiments and ESI-FTICR-MS(n). Their configurations were determined by Marfey's method and J-based NMR analysis. These new metabolites inhibited the growth of human cell lines (A549, HeLa, LNCaP, PC3, and NFF) with IC(50) values ranging from 88 to 370 nM. However, ne hamide D causes A549 cell proliferation at subcytotoxic doses and should be treated cautiously as a cytotoxic compound.
Publisher: Springer Science and Business Media LLC
Date: 24-04-2007
DOI: 10.1007/S00239-006-0146-3
Abstract: Many rDNA molecular phylogenetic studies result in trees that are incongruent to either alternative gene tree reconstructions and/or morphological assumptions. One reason for this outcome might be the application of suboptimal phylogenetic substitution models. While the most commonly implemented models describe the evolution of independently evolving characters fairly well, they do not account for character dependencies such as rRNA strands that form a helix in the ribosome. Such nonindependent sites require the use of models that take into account the coevolution of the complete nucleotide pair (doublet). We analyzed 28S rDNA (LSU) demosponge phylogenies using a "doublet" model for pairing sites (rRNA-helices) and compared our findings with the results of "standard" approaches using Bayes factors. We demonstrate that paired and unpaired sites of the same gene result in different reconstructions and that usage of a doublet model leads to more reliable demosponge trees. We show the influence of more sophisticated models on phylogenetic reconstructions of early-branching metazoans and the phylogenetic relationships of demosponge orders.
Publisher: Elsevier BV
Date: 06-2008
Publisher: Elsevier BV
Date: 11-2003
Publisher: American Chemical Society (ACS)
Date: 04-11-2013
DOI: 10.1021/NP400617H
Abstract: Chemical investigations of two specimens of Trikentrion flabelliforme collected from Australian waters have resulted in the identification of four new indole alkaloids, trikentramides A-D (9-12). The planar chemical structures for 9-12 were established following analysis of 1D/2D NMR and MS data. The relative configurations for 9-12 were determined following the comparison of (1)H NMR data with data previously reported for related natural products. The application of a quantum mechanical modeling method, density functional theory, confirmed the relative configurations and also validated the downfield carbon chemical shift observed for one of the quaternary carbons (C-5a) in the cyclopenta[g]indole series. The indole-2,3-dione motif present in trikentramides A-C is rare in nature, and this is the first report of these oxidized indole derivatives from a marine sponge.
Publisher: Oxford University Press (OUP)
Date: 19-10-2006
Abstract: The first mitochondrial (mt) genomes of demosponges have recently been sequenced and appear to be markedly different from published eumetazoan mt genomes. Here we show that the mt genome of the haplosclerid demosponge Amphimedon queenslandica has features that it shares with both demosponges and eumetazoans. Although the A. queenslandica mt genome has typical demosponge features, including size, long noncoding regions, and bacterialike rRNA genes, it lacks atp9, which is found in the other demosponges sequenced to date. We found strong evidence of a recent transposon-mediated transfer of atp9 to the nuclear genome. In addition, A. queenslandica bears an incomplete tRNA set, unusual amino acid deletion patterns, and a putative control region. Furthermore, the arrangement of mt rRNA genes differs from that of other demosponges. These genes evolve at significantly higher rates than observed in other demosponges, similar to previously observed nuclear rRNA gene rates in other haplosclerid demosponges.
Publisher: American Chemical Society (ACS)
Date: 07-1999
DOI: 10.1021/JO990404D
Abstract: Adociasulfate 1 (1), adociasulfate 7 (2), and adociasulfate 8 (3), which are inhibitors of proton pump activity in hen bone-derived membrane vesicles, were isolated from an extract of the sponge Adocia sp. (Chalinidae). Structure elucidation by 2D-NMR spectroscopy revealed that they are novel hexaprenoid hydroquinone sulfates.
Publisher: Oxford University Press (OUP)
Date: 04-01-2017
DOI: 10.1093/HMG/DDW418
Abstract: To understand the cause of Parkinson's disease (PD), it is important to determine the functional interactions between factors linked to the disease. Parkin is associated with autosomal recessive early-onset PD, and controls the transcription of PGC-1α, a master regulator of mitochondrial biogenesis. These two factors functionally interact to regulate the turnover and quality of mitochondria, by increasing both mitophagic activity and mitochondria biogenesis. In cortical neurons, co-expressing PGC-1α and Parkin increases the number of mitochondria, enhances maximal respiration, and accelerates the recovery of the mitochondrial membrane potential following mitochondrial uncoupling. PGC-1α enhances Mfn2 transcription, but also leads to increased degradation of the Mfn2 protein, a key ubiquitylation target of Parkin on mitochondria. In vivo, Parkin has significant protective effects on the survival and function of nigral dopaminergic neurons in which the chronic expression of PGC-1α is induced. Ultrastructural analysis shows that these two factors together control the density of mitochondria and their interaction with the endoplasmic reticulum. These results highlight the combined effects of Parkin and PGC-1α in the maintenance of mitochondrial homeostasis in dopaminergic neurons. These two factors synergistically control the quality and function of mitochondria, which is important for the survival of neurons in Parkinson's disease.
Publisher: Elsevier BV
Date: 03-2009
DOI: 10.1016/J.BMC.2009.01.065
Abstract: Bioassay-guided fractionation of the organic extract from the marine sponge Acanthella costata resulted in the isolation of the known natural product, (-)-dibromophakellin (1). Using a fluorescence imaging plate reader (FLIPR) based assay, compound 1 was identified as displaying agonist activity against the alpha(2B) adrenoceptor, with an EC(50) of 4.2muM. Debromination and Suzuki-Miyaura coupling reactions were undertaken in order to provide structure activity data about the pyrrole ring of this marine metabolite. These synthetic studies generated the known natural product analogues, (-)-phakellin (2), and (-)-monobromophakellin (3), along with the new synthetic derivatives (-)-4-bromo-5-phenylphakellin (5) and (-)-4,5-diphenylphakellin (6). Substitution of the C-5 Br of 1 with H (2 and 3) or phenyl (5 and 6) resulted in loss of activity indicating that Br at C-5 is required for agonist activity.
Publisher: Cambridge University Press (CUP)
Date: 12-11-2016
DOI: 10.1017/S0025315415001721
Abstract: Suitable genetic markers for population studies in sponges are necessary to further our understanding of bio ersity and dispersal patterns, and contribute to conservation efforts. Due to the slow mitochondrial substitution rates in demosponges, nuclear introns are among the preferable markers for phylogeographic studies, but so far only the second intron of the ATP synthetase beta subunit-gene (ATPSβ) has been successfully established. In the present study, we analyse the intron of the Lysidyl Aminoacyl Transfer RNA Synthetase (LTRS), another potential marker to study demosponge intraspecific relationships, on s les of Neopetrosia chaliniformis from various locations in the Indo-Pacific and compare its variation with a mitochondrial marker (CO2). LTRS recovers several reciprocal monophyletic groups among the Indo-Pacific N. chaliniformis and provides a potential alternative to ATPSβ.
Publisher: CSIRO Publishing
Date: 2002
DOI: 10.1071/MF00153
Abstract: The influence of environmental variability on body form and tissue structure of Cinachyrella australiensis is reported for populations from three sites within Darwin Harbour, Northern Territory, Australia, that varied considerably in hydrological conditions. External morphology of these sponges differed among sites ranging from typical spherical shapes to flattened forms. A large proportion of dry weight consisted of inorganic matter, i.e. silica spicules, varying between 62.9% and 78.2%. Sites with highest water velocity and sediment size were significantly correlated with sponge populations having the greatest inorganic content and lowest organic cellular content and the thickest oxea. Thicker oxea may in part account for the higher structural content of sponges at these sites. There was no significant difference in oxea length among sites. It is concluded that sponges subjected to highly perturbed environs with large water flow and sedimentation regimes may devote more energy to spicule reinforcement relative to organic content. These robust sponges have the potential to make an important structural contribution to their habitats.
Publisher: Springer Science and Business Media LLC
Date: 03-1993
DOI: 10.1007/BF01923536
Publisher: Elsevier BV
Date: 2010
Publisher: American Chemical Society (ACS)
Date: 05-2005
DOI: 10.1021/NP049595S
Abstract: Bioassay-guided fractionation of the MeOH extract of the sponge Oceanapia sp. using the Helicobacter pylori enzyme, aspartyl semialdehyde dehydrogenase, ASD, to detect antibacterial activity, led to the isolation of a new pyridoacridine alkaloid, petrosamine B (1). Petrosamine B is a bright blue compound that is sparingly soluble in many organic solvents. The structure of 1 was determined from detailed NMR studies performed in TFA/D2O. Petrosamine B was found to be a weak inhibitor of ASD with an IC50 of 306 microM.
Publisher: American Chemical Society (ACS)
Date: 24-03-2000
DOI: 10.1021/NP9904657
Abstract: The combined CH(2)Cl(2) and MeOH crude extract of a new species of the marine sponge Dysidea, collected in Northern Australia was found to inhibit the binding of [I125] interleukin-8 [IL-8] to the human recombinant IL-8 receptor type A at 500 microg/mL. Bioassay-guided fractionation led to the isolation of three new polyoxygenated sterols 3, 4, and 5. Their structures were assigned on the basis of 1D and 2D NMR experiments, and relative stereochemistries were established by ROESY correlations and analysis of coupling constants. The IC(50) values for inhibition of IL-8Ra for sterols 3, 4, and 5 were 20, 5.5, and 4.5 microM, respectively.
Publisher: CSIRO Publishing
Date: 2010
DOI: 10.1071/CH09617
Abstract: The new metabolite psammaplin N (8) that contains a sulfoxide moiety has been isolated together with psammaplins A (1), I (4a), and J (2) from a specimen of the marine sponge Aplysinella rhax (order Verongida family Aplysinidae) collected in South East Queensland. An NMR study on psammaplin J reveals its facile conversion to psammaplins A and I when exposed to methanol.
Publisher: Magnolia Press
Date: 11-06-2014
DOI: 10.11646/ZOOTAXA.3814.4.1
Abstract: Extensive surveys of the bio ersity on the seafloor of the inter-reef regions of the Great Barrier Reef, Australia, have resulted in the collection of large numbers of sponges, many of which are likely new to science. Identification of these sponges, however, was made difficult by the absence in some specimens of key diagnostic characters, such as megascleres. We used an integrated approach to the taxonomy of these sponges, incorporating morphological examination by SEM, analysis of DNA sequence data (using the COI barcoding fragment of mtDNA) and preliminary studies of the chemistry of the sponges, to describe the new species, which were found to contain no native spicules other than acanthose microrhabds. Here, we propose two new species of Theonella Gray, 1868: Theonella deliqua n. sp. (found in association with a single unidentified species of siliquariid mollusc) and Theonella maricae n. sp. from the Great Barrier Reef. Further, we propose the new combination of Theonella xantha (Sutcliffe, Hooper and Pitcher 2010) n. comb. for another microrhabd-only-bearing species. On the basis of our gene trees, we recognise Theonella (and Theonellidae Lendenfeld, 1903) within Astrophorida Sollas, 1887. We discuss the potential for chemotaxonomic and DNA-based insights into the origins and radiation of species of Theonella and explore the evolutionary significance of the reduced morphology of the three additional species recognised here.
Publisher: Springer Science and Business Media LLC
Date: 03-2012
Publisher: Magnolia Press
Date: 12-05-2020
DOI: 10.11646/ZOOTAXA.4774.1.1
Abstract: This research presents 17 new species of carnivorous sponges from the family Cladorhizidae s led at bathyal and abyssal depths off the east coast of Australia during the RV Investigator 2017 Abyss Expedition. The species are comprised of six genera: Abyssocladia escheri sp. nov., A. annae sp. nov., A. gliscofila sp. nov., Asbestopluma (Asbestopluma) maxisigma sp. nov., Cladorhiza australis nov. sp., C. poritea sp. nov., C. investigator sp. nov., C. moniqueae sp. nov., C. pentaeiros sp. nov., Chondrocladia (Chondrocladia) freycinetensis sp. nov., Ch. (Ch.) callistemonex sp. nov., Ch. (Ch.) zygainadentonis sp. nov., Lycopodina nikitawimandi sp. nov., L. helios sp. nov., L. cassida sp. nov., L. brochidodroma sp. nov. and Euchelipluma claudochela sp. nov. Only three carnivorous species, A. desmophora, As. (As.) desmophora and C. (Meliiderma) tasmaniensis, have been recorded from Australia previously. This expedition also recovered two described species commonly recorded from the Southern Hemisphere Ch. (Ch.) clavata and L. calyx. This paper also redescribes two species from the South Pacific i.e. C. mirabilis and C. similis.
Publisher: MDPI AG
Date: 22-07-2015
DOI: 10.3390/MD13074520
Publisher: Elsevier BV
Date: 12-2007
DOI: 10.1016/J.BMCL.2007.10.021
Abstract: Isoprenylcysteine methyltransferase (Icmt) catalyzes the carboxyl methylation of oncogenic proteins in the final step of a series of post-translational modifications. The inhibition of Icmt provides an attractive and novel anticancer target. A natural product high-throughput screening c aign was conducted to discover inhibitors of Icmt. The Australian marine sponge, Pseudoceratina sp., yielded spermatinamine, a novel alkaloid with a bromotyrosyl-spermine-bromotyrosyl sequence, as the bioactive constituent. Its structure was determined by 1D and 2D NMR spectroscopy. Spermatinamine is the first natural product inhibitor of Icmt.
Publisher: Elsevier BV
Date: 06-1996
Publisher: Springer Science and Business Media LLC
Date: 21-03-2017
Publisher: American Chemical Society (ACS)
Date: 15-07-2016
DOI: 10.1021/ACS.JNATPROD.6B00168
Abstract: Four bicyclic and three pentacyclic guanidine alkaloids (1-7) were isolated from a French Polynesian Monanchora n. sp. sponge, along with the known alkaloids monalidine A (8), enantiomers 9-11 of known natural product crambescins, and the known crambescidins 12-15. Structures were assigned by spectroscopic data interpretation. The relative and absolute configurations of the alkaloids were established by analysis of (1)H NMR and NOESY spectra and by circular dichroism analysis. The new norcrambescidic acid (7) corresponds to interesting biosynthetic variation within the pentacyclic core. All compounds exhibited antiproliferative and cytotoxic efficacy against KB, HCT116, HL60, MRC5, and B16F10 cancer cells, with IC50 values ranging from 4 nM to 10 μM.
Publisher: MDPI AG
Date: 23-10-2019
DOI: 10.3390/MD17110598
Abstract: There is an urgent need to discover and develop new anthelmintics for the treatment of parasitic nematodes of veterinary importance to circumvent challenges linked to drug resistant parasites. Being one of the most erse natural ecosystems, the marine environment represents a rich resource of novel chemical entities. This study investigated 2000 extracts from marine invertebrates, collected from Australian waters, for anthelmintic activity. Using a well-established in vitro bioassay, these extracts were screened for nematocidal activity against Haemonchus contortus — a socioeconomically important parasitic nematode of livestock animals. Extracts (designated Mu-1, Ha-1 and Ha-2) from two marine sponges (Monanchora unguiculata and Haliclona sp.) each significantly affected larvae of H. contortus. In idual extracts displayed a dose-dependent inhibition of both the motility of exsheathed third-stage larvae (xL3s) and the development of xL3s to fourth-stage larvae (L4s). Active fractions in each of the three extracts were identified using bioassay-guided fractionation. From the active fractions from Monanchora unguiculata, a known pentacyclic guanidine alkaloid, fromiamycalin (1), was purified. This alkaloid was shown to be a moderately potent inhibitor of L4 development (half-maximum inhibitory concentration (IC50) = 26.6 ± 0.74 µM) and L4 motility (IC50 = 39.4 ± 4.83 µM), although it had a relatively low potency at inhibiting of xL3 motility (IC50 ≥ 100 µM). Investigation of the active fractions from the two Haliclona collections led to identification of a mixture of amino alcohol lipids, and, subsequently, a known natural product halaminol A (5). Anthelmintic profiling showed that 5 had limited potency at inhibiting larval development and motility. These data indicate that fromiamycalin, other related pentacyclic guanidine alkaloids and/or halaminols could have potential as anthelmintics following future medicinal chemistry efforts.
Publisher: Elsevier BV
Date: 12-1993
Publisher: Cambridge University Press (CUP)
Date: 12-2007
DOI: 10.1017/S0025315407058195
Abstract: We present the most comprehensive cytochrome oxidase subunit 1 gene tree published to date for demosponges based on new sequences. The CO1 barcoding fragment is sequenced for 65 species from the Caribbean Sea, and its gene tree reconstructed. Although its deeper nodes are not particularly well-supported, the gene tree provides a variety of information for new phylogenetic patterns, as well as support for previously published 28S rDNA gene trees. In our analysis Halichondriidae cluster with Suberitidae, supporting previous 28S rDNA data. Chelae-bearing Poecilosclerida are monophyletic but most taxa lacking chelae in this dataset cluster more distantly. Haplosclerida are not resolved monophyletically under this fragment. While some species exhibit distinct barcodes, some genera contain species that share CO1 haplotypes.
Publisher: American Chemical Society (ACS)
Date: 18-06-2008
DOI: 10.1021/NP8001628
Publisher: Cambridge University Press (CUP)
Date: 04-05-2015
DOI: 10.1017/S0025315415000521
Abstract: Sponge species are infamously difficult to identify for non-experts due to their high morphological plasticity and the paucity of informative morphological characters. The use of molecular techniques certainly helps with species identification, but unfortunately it requires prior reference sequences. Holotypes constitute the best reference material for species identification, however their usage in molecular systematics and taxonomy is scarce and frequently not even attempted, mostly due to their antiquity and preservation history. Here we provide case studies in which we demonstrate the importance of using holotype material to answer phylogenetic and taxonomic questions. We also demonstrate the possibility of sequencing DNA fragments out of century-old holotypes. Furthermore we propose the deposition of DNA sequences in conjunction with new species descriptions.
Publisher: Magnolia Press
Date: 28-05-2021
DOI: 10.11646/ZOOTAXA.4979.1.8
Abstract: The peer-reviewed journal Zootaxa has accelerated the rate of sponge (Porifera) species discoveries in 289 peer-reviewed papers published between 2002 up until the end of 2020, describing 725 new species, six new subspecies, 27 new genera, four new subgenera, and 123 new species and genus names needed to resolve existing homonyms. Zootaxa has been the most prolific of all taxonomic journals in its contributions to describing new taxa of Porifera in modern times. This present article analyses these taxonomic contributions over the past 20 years of Zootaxa, including their trends and highlights pertaining to sponge publications.
Publisher: Magnolia Press
Date: 13-11-2020
DOI: 10.11646/ZOOTAXA.4878.2.2
Abstract: This research presents three new species of carnivorous sponges from the family Cladorhizidae from the Great Australian Bight, South Australia. This research also shows a clear separation within the species currently known as Cladorhiza into those with an arbuscular or tree-like morphology from the Atlantic, to those of differing morphologies and propose three new genera, i.e. Bathytentacular gen. nov., Abyssosdiskos gen. nov. and Nullarbora gen. nov. and the resurrection of an old genus Axoniderma. nov. The three new species described in this paper are Nullarbora heptaxia sp. nov., Abyssocladia oxyasters sp. nov. and Lycopodina hystrix sp. nov. A new species in the family Guitarridae, Guitarra davidconryi sp. nov., a family closely related to the carnivorous sponges is also described in this paper. These new species are the first recorded carnivorous species from South Australia and increase the number of species recorded from around Australia to 25.
Publisher: Elsevier BV
Date: 09-2014
Publisher: American Chemical Society (ACS)
Date: 20-04-2009
DOI: 10.1021/NP8008013
Abstract: Three new marine alkaloids, clavatadines C-E (1-3), together with the three known compounds aerophobin 1 (4), purealdin L (5), and aplysinamisine II (6) were isolated from extracts of the sponge Suberea clavata by bioassay-guided fractionation using a serine protease factor XIa assay. Their structures were determined by 1D and 2D NMR spectroscopy. Compounds 1-6 exhibited weak inhibition of factor XIa.
Publisher: Public Library of Science (PLoS)
Date: 03-07-2012
Publisher: Hindawi Limited
Date: 28-01-2020
DOI: 10.1111/JZS.12351
Publisher: American Chemical Society (ACS)
Date: 30-05-2008
DOI: 10.1021/JM800314B
Abstract: Bioassay-guided fractionation of a CH2Cl2/MeOH extract of the sponge Suberea clavata using the serine protease factor XIa to detect antithrombotic activity led to the isolation of the new marine natural products, clavatadines A and B. Clavatadines A and B inhibited factor XIa with IC50's of 1.3 and 27 microM, respectively. A crystal structure of protein-inhibitor (clavatadine A) complex was obtained and revealed interesting selective binding and irreversible inhibition of factor XIa. The cocrystal structure provides guidance for the design and synthesis of future factor XIa inhibitors as antithrombotic agents.
Publisher: Magnolia Press
Date: 23-05-2023
DOI: 10.11646/ZOOTAXA.5293.3.2
Abstract: This research presents three new species of carnivorous sponges from the family Cladorhizidae from the Great Barrier Reef, in Queensland, Australia: Abyssocladia falkor sp. nov., Abyssocladia jeanvaceleti sp. nov. and Axoniderma wanda sp. nov. They were collected by ROV during the expedition FK200802—Seamounts, Canyons & Reefs of the Coral Sea Cruise on the RV Falkor from the Schmidt Ocean Institute. In addition, the ROV collection of two complete specimens enabled the redescription of two other Australian species of carnivorous sponge (Chondrocladia (Chondrocladia) zygainadentonis Ekins et al., 2020a and Asbestopluma (Asbestopluma) maxisigma Ekins et al., 2020a), previously known from the East coast of Australia based on incomplete specimens.
Publisher: American Chemical Society (ACS)
Date: 21-11-2007
DOI: 10.1021/NP070366Q
Abstract: As part of our studies to discover P2X 7 receptor antagonists, the sponge Callyspongia sp. was investigated. A new tripyridine alkaloid niphatoxin C ( 1) was isolated and had P2X 7 receptor antagonism however, cytotoxicity of THP-1 cells was the predominant biological effect at higher concentrations. Its structure was determined by 1- and 2-D NMR spectroscopy.
Publisher: PeerJ
Date: 24-09-2018
DOI: 10.7717/PEERJ.5586
Abstract: Correct identification and classification of sponges is challenging due to ambiguous or misleading morphological features. A particular case is a blue keratose sponge occasionally referred to as the “Blue Photo Sponge” among aquarists, which appears frequently (and in several cases unintended) in private aquaria. This spicule-less species, occasionally specified as Collospongia auris Bergquist, Cambie & Kernan 1990, not only displays a high phenotypic plasticity in growth form and colour, it also proliferates in aquacultures under standard conditions unlike most other sponges. Therefore, this species is regarded as a pest for most aquarists. In turn, the ease of cultivation and propagation in aquacultures qualifies this species as a model organism for a wide array of scientific applications. For these purposes, correct identification and classification are indispensable. We reconstructed ribosomal gene trees and determined this species as Lendenfeldia chondrodes (De Laubenfels, 1954) (Phyllospongiinae), distant to Collospongia auris , and corroborated by skeletal features. Additionally, the resulting phylogeny corroborated major shortcomings of the current Phyllospongiinae classification—its consequences are discussed.
Publisher: CSIRO Publishing
Date: 2012
DOI: 10.1071/CH12010
Abstract: The isolation and structure elucidation of seven new oxygenated terpenoids and eight known terpene metabolites from marine invertebrates collected at the Inner Gneerings Reef, South East Queensland, is discussed. Investigation of the sponge Coscinoderma matthewsi yielded an epoxylactone derivative (1) of the known furanoterpene tetradehydrofurospongin-1 (2). A chemical investigation of the dissected nudibranch Chromodoris albopunctata provided the new oxygenated diterpenes 12α-acetoxyspongian-16-one (10), 20-acetoxyspongian-16-one (12), 20-oxyspongian-16-one propionate (13) and 12α,20-dioxyspongian-16-one dipropionate (14) in conjunction with three other known diterpene metabolites, while two new chromodorolides, D (17) and E (18), in addition to four known diterpenes were isolated from a Dysidea sp.
Publisher: Wiley
Date: 15-04-2014
Publisher: Cambridge University Press (CUP)
Date: 12-2007
DOI: 10.1017/S0025315407058183
Abstract: The ribosome is the location of protein translation and therefore a pivotal macromolecular complex for all organisms. The RNA molecules involved in the formation and functioning of the ribosome (rRNA) are partially single-stranded (loops) and partially double-stranded (helices or stems) as a result of pairing of complementary regions in either their own or other rRNA subunits. This pattern provides the rRNA with a secondary structure crucial for its functionality. The stability of these secondary structures is mediated by their base compositions: a helix rich in G-C pairs possesses a higher thermodynamic stability than an A-T rich counterpart. However, the base composition of these structures is neither homogeneous throughout the molecule nor throughout the demosponge taxa. Here, we present patterns of biased nucleotide composition in demosponge 28S rDNA. We analyse their correlation in respect to environment and taxonomy. We find significantly higher G+C contents in haplosclerid demosponges compared to other orders and investigate evidence for an association between water temperature and rRNA base composition in demosponges.
Publisher: Inter-Research Science Center
Date: 2007
DOI: 10.3354/MEPS340073
Publisher: Springer Science and Business Media LLC
Date: 2002
Publisher: Cambridge University Press (CUP)
Date: 09-09-2016
DOI: 10.1017/S0025315415001149
Abstract: The Indonesian archipelago is a ‘hotspot’ for invertebrate bio ersity (‘Coral Triangle’). In this area of ‘peak’ bio ersity, the origins of this high species ersity have often been debated. Xestospongia testudinaria is one of the sponge species that dominates coral reef sponge communities in this region. The role of the so-called ‘giant barrel sponge’ for the reef ecosystem has been studied repeatedly, as have its various bioactive compounds. However, the genetic variation of this iconic sponge in the region remains unknown. We investigate over 200 barrel sponge s les from Indonesia, and neighbouring as well as more distant localities (Saudi Arabia, Tanzania, Thailand, Taiwan, Java, Sulawesi and the Great Barrier Reef, Australia) using the mitochondrial cytochrome oxidase subunit 1. We compare our results with those from the studies on the congeneric barrel sponges Xestospongia muta from the Caribbean, and Xestospongia bergquistia from the Indo-Pacific, and observe a high degree of overlapping haplotypes between the three barrel sponge species, likely indicating the presence of ancestral polymorphisms. We discuss the implications of these findings to better interpret the phylogeography of barrel sponge taxa in the Indo-Pacific.
Publisher: Elsevier BV
Date: 03-2004
Publisher: American Chemical Society (ACS)
Date: 23-10-2007
DOI: 10.1021/NP070156D
Abstract: Specimens of the Indo-Pacific sponge Acanthella cavernosa Dendy collected from locations along the Eastern coastline of Australia have been shown to contain a range of sesquiterpenes including isothiocyanate 1, isocyanide 10, and the isocyanates 15 and 22. These metabolite studies have provided a basis for chemical comparisons between sponge populations from different geographic locations and between in idual specimens collected from a single location.
Publisher: Public Library of Science (PLoS)
Date: 07-01-2015
Publisher: American Chemical Society (ACS)
Date: 22-02-2007
DOI: 10.1021/JO062007Q
Abstract: The distribution of the P2X7 receptor in inflammatory cells suggests that P2X7 antagonists have a significant role to play in the treatment of inflammatory disease. We conducted a natural product high-throughput screening c aign to discover P2X7 receptor antagonists. The Australian marine sponge Stylissa flabellata yielded two new bisimidazo-pyrano-imidazole bromopyrrole ether alkaloids, stylissadines A (IC50 0.7 microM) and B (IC50 1.8 microM), as the specific bioactive constituents. The compounds inhibit BzATP-mediated pore formation in THP-1 cells. Also present in this extract was considerable nonspecific bioactivity in the hemeolysin specificity assay. A new pyrrole-imidazole alkaloid, konbu'acidin B, and the known pyrrole-imidazole alkaloids 4,5-dibromopalau'amine and massadine were also isolated and had nonspecific activity. ROESY and proton coupling constant data indicated that the stereochemistry at C12, C17, and C20 in 4,5-dibromopalau'amine should be revised to 12R, 17S, 20S. By analogy, the relative stereochemistry of palau'amine, 4-bromopalau'amine, styloguanidine, 3-bromostyloguanidine, and 2,3-dibromostyloguanidine should also be revised to 12R, 17S, 20S. Stylissadines A and B are the most potent natural product P2X7 antagonists to be isolated to date and provide a novel class of P2X7 receptor inhibitors. They are also the first ex les of tetrameric pyrrole-imidazole alkaloids.
Publisher: Elsevier BV
Date: 04-2014
DOI: 10.1016/J.YMPEV.2014.01.023
Abstract: The octocorals of the Ellisellidae constitute a erse and widely distributed family with sub isions into genera based on colonial growth forms. Branching patterns are repeated in several genera and congeners often display region-specific variations in a given growth form. We examined the systematic patterns of ellisellid genera and the evolution of branching form ersity using molecular phylogenetic and ancestral morphological reconstructions. Six of eight included genera were found to be polyphyletic due to biogeographical incompatibility with current taxonomic assignments and the creation of at least six new genera plus several reassignments among existing genera is necessary. Phylogenetic patterns of ersification of colony branching morphology displayed a similar transformation order in each of the two primary ellisellid clades, with a sea fan form estimated as the most-probable common ancestor with likely origins in the Indo-Pacific region. The observed parallelism in evolution indicates the existence of a constraint on the genetic elements determining ellisellid colonial morphology. However, the lack of correspondence between levels of genetic ergence and morphological ersity among genera suggests that future octocoral studies should focus on the role of changes in gene regulation in the evolution of branching patterns.
Publisher: MDPI AG
Date: 21-05-2020
DOI: 10.3390/MD18050272
Abstract: Four new brominated tyrosine metabolites, aplyzanzines C–F (1–4), were isolated from the French Polynesian sponge Pseudoceratina n. sp., along with the two known 2-aminoimidazolic derivatives, purealidin A (5) and 6, previously isolated, respectively, from the sponges Psammaplysilla purpurea and Verongula sp. Their structures were assigned based on the interpretation of their NMR and HRMS data. The compounds exhibited quorum sensing inhibition (QSi) and antifouling activities against several strains of bacteria and microalgae. To our knowledge, the QSi activity of this type of bromotyrosine metabolite is described here for the first time.
Publisher: Elsevier BV
Date: 06-2012
DOI: 10.1016/J.YMPEV.2012.02.024
Abstract: The demosponge orders Dictyoceratida and Dendroceratida are historically assigned to the keratose (or "horny") sponges, which are mostly devoid of primary skeletal elements, but possess an elaborate skeleton of organic fibres instead. This paucity of complex mineral skeletal elements makes their unambiguous classification and phylogenetic reconstruction based on morphological features difficult. Here we present the most comprehensive molecular phylogeny to date for the Dendroceratida, Dictyoceratida, and also other sponge orders that largely lack a mineral skeleton or skeletal elements at all (i.e. Verongida, Halisarcida, Chondrosida), based on independent mitochondrial and nuclear markers. We used molecular data to validate the coherence of all recognised orders, families and subfamilies that are currently defined using morphological characteristics. We discussed the significance of morphological and chemotaxonomic characters for keratose sponges, and suggested adapted definitions for the classification of dendroceratid, dictyoceratid, and verongid higher taxa. Also, we found that chondrosid sponges are non-monophyletic with respect to Halisarcida. Verongida and Dendroceratida were monophyletic, however most of their classically recognised families were not recovered. This indicated that the current distinction between dendritic and mesh-like fibre skeletons is not significant at this level of classification. Dysideidae were found to be the sister-group to the remaining Dictyoceratida. Irciniidae formed a distinct clade, however Thorectidae and Spongiidae could not be separated with the molecular markers used. Finally, we are establishing the name Verongimorpha for the clade combining verongid, chondrosid and halisarcid taxa and readjust the content of its sister-clade Keratosa.
Publisher: Springer Science and Business Media LLC
Date: 10-05-2023
DOI: 10.1007/S10531-023-02613-Y
Abstract: The distribution of marine sponges in the tropical Southwest Pacific Ocean is largely unexplored despite the vital ecological role of sponges in coral reefs and their value as sources of metabolites for drug design. Several collection c aigns to the French Polynesian archipelagos (Society, Marquesas, Tuamotu, Gambier, and Austral) were conducted to assess the bio- and chemo ersity of the island groups. In the course of these scientific expeditions, more than 200 identified sponge specimens were acquired, for which we were able to assign 102 Molecular Operational Taxonomic Units (MOTUs). Based on these MOTUs, we assessed, in the largest analysis of its kind for this area to date, the sponge composition and faunistic overlaps of the marine province Southeast Polynesia with Marquesas and Central Polynesia. We also compared the sponge fauna of these Eastern Indo-Pacific provinces with marine provinces of the adjacent Central Indo-Pacific realm. Our findings corroborate that sponge faunal similarity within marine realms is higher than among realms, and follows the marine barriers to gene flow observed for other taxa. We detected high levels of provincial endemism for marine sponges, consistent with findings from other Indo-Pacific regions. At the level of province, geographical distance and ocean surface currents influence faunal similarity, and constitute the primary factors for the connectivity of sponge faunas between the disjunct and remote island groups in the tropical Southwest Pacific Ocean.
Publisher: Springer Science and Business Media LLC
Date: 22-05-2013
DOI: 10.1038/NATURE12188
Publisher: Springer Science and Business Media LLC
Date: 05-02-2012
Publisher: Pensoft Publishers
Date: 08-11-2012
Publisher: American Chemical Society (ACS)
Date: 24-01-2011
DOI: 10.1021/NP100620X
Abstract: Sixteen new compounds, comprising nine new plakortolides K-S (1-9), four seco-plakortolides (10-13), and three plakortones (14-16), were isolated from the Australian sponge Plakinastrella clathrata. Structural elucidation, including relative configurational assignment, was based on extensive spectroscopic analysis, while the absolute configurations of 1-4 were deduced from (1)H NMR analyses on MPA esters derived from Zn/AcOH reduction products. Diastereomeric sets of plakortolides, e.g., K and L, or M and N, differed in configuration at C-3/C-4 rather than at C-6, a stereochemical result that compromises a biosynthetic pathway involving Diels-Alder cycloaddition of molecular oxygen to a Δ(3,5)-diunsaturated fatty acid precursor. The biosynthesis may plausibly involve cyclization of a 6-hydroperoxydienoic acid intermediate following stereospecific introduction of the hydroperoxy group into a polyketide-derived precursor. Isolated seco-plakortolides converted under mild conditions into plakortones with full retention of configuration, suggesting C-6 hydroxy attack on an α,β-unsaturated lactone intermediate. The NMR data reported for the compound named plakortolide E are inconsistent with the current literature structure and are those of the corresponding seco-plakortolide (19). The reported conversion of the metabolite into a plakortone ether on storage is consistent with this structural revision.
Publisher: Magnolia Press
Date: 30-10-2014
DOI: 10.11646/ZOOTAXA.3878.6.5
Abstract: The subgenus Clathria (Thalysias) Duchassaing & Michelotti, 1864 has 97 valid species, of which 27 are recorded in the Atlantic Ocean. However, only three species are known from Brazil so far. Here we provide the redescription of Clathria (Thalysias) basiarenacea (Boury- Esnault, 1973), based on the discovery of new characters (additional category of auxiliary styles, and details of spicules), and describe a new species of Clathria (Thalysias) repens sp. nov., that differs from sister species in having a live orange color, a massively encrusting repent growth form with lamellate folds and anastomosed projections, and three categories of structural styles, of which the two auxiliary styles have microspined heads. We also invalidate the record of Clathria (Thalysias) procera (Ridley, 1884) for Brazil.
Publisher: American Chemical Society (ACS)
Date: 19-05-2010
DOI: 10.1021/NP100010Z
Abstract: Three new bastadins, bastadin 25 (1), 15-O-sulfonatobastadin 11 (2), and bastadin 26 (3), were isolated from a MeOH extract of the Australian marine sponge Ianthella flabelliformis. Their structures were determined by interpretation of 1D and 2D NMR spectra and mass spectrometry. Bastadin 26 (3) showed potent affinity for the guinea pig delta-opioid receptors with a K(i) value of 100 nM. The other two bastadins had a 100-fold lower affinity. The three compounds were also tested for their affinity to guinea pig micro- and kappa-opioid receptors and shown to have either no affinity or only very weak affinity toward both of these opioid receptors.
Publisher: Wiley
Date: 22-10-2008
Publisher: Wiley
Date: 04-2023
DOI: 10.1111/MAEC.12747
Abstract: Sponges host a wide range of cryptic bio ersity both internally and externally. This study quantified the bio ersity inhabiting, or associated with, three morphologically distinct coral reef sponges. Coelocarteria singaporensis , Coscinoderma mathewsi and Ircinia microconulosa were collected from fringing reefs at Orpheus Island, central Great Barrier Reef, across monsoonal seasons (wet and dry) and diurnal periods (morning, afternoon and night for C . singaporensis ) to quantify Sponge Associated Biota (SAB). A total of 8489 SAB was sorted from 77 sponges. Twenty‐six taxa, across 3 kingdoms and 12 phyla were recorded. Sponge‐associated biota was dominated by Mollusca in all three sponge hosts (range ~ 46%–50%) but taxa were found from a number of other phyla including, Arthropoda, Annelida, Echinodermata, Cnidaria, Foraminifera and Nemertea. There was no effect of season on abundances of SAB within the three species of sponge, but there was a significant effect associated with sponge host species, driven by a higher number of SAB in C . singaporensis and I . microconulosa . The diurnal patterns of SAB within C . singaporensis showed consistent abundances of mobile invertebrate groups with molluscs, echinoderms and arthropods dominating these s les. This study also highlights the ecological role and contribution of SAB in the reef ecosystems.
Publisher: MDPI AG
Date: 04-06-2014
DOI: 10.3390/MD12063399
Publisher: American Chemical Society (ACS)
Date: 28-02-2004
DOI: 10.1021/NP0204592
Abstract: By use of bioassay (murine P388 lymphocytic leukemia cell line) guided isolation procedures, extracts of the Republic of Singapore marine sponge Hymeniacidon sp. were found to contain demethyloxyaaptamine (1) and aaptamine (3) as prominent cancer cell growth inhibitory constituents accompanied by the trace, albeit more active, component isoaaptamine (4). The isolation, X-ray structure elucidation, and antineoplastic and antimicrobial activities of isoaaptamine (4) have been summarized. Because of instability, isoaaptamine (4) was converted to a stable sodium phosphate prodrug designated hystatin 1 (7).
Publisher: American Chemical Society (ACS)
Date: 14-02-2002
DOI: 10.1021/NP010383U
Abstract: The polar extract of the Vanuatu sponge Reniera n. sp., which showed immunomodulating activity in preliminary tests, was found to contain a cyclic tripeptide, which we named renieramide (1). This metabolite is identical to a synthetic derivative mentioned in a patent concerning the preparation of cyclic peptides of the OF4949 family of anticancer agents. We describe here the first isolation of this metabolite from natural sources and its complete characterization by spectroscopic and chemical approaches. Renieramide (1) possesses a 17-membered cyclic side-chain-linked biphenyl ether skeleton, typical of the class that includes the natural products OF4949 I-IV, K13, and eurypamides. A tridimensional model of 1, obtained by NMR restrained molecular mechanics and dynamics, is also presented.
Publisher: Springer Science and Business Media LLC
Date: 31-08-2017
Publisher: Cambridge University Press (CUP)
Date: 09-11-2005
DOI: 10.1017/S0025315405012543
Abstract: The genus Desmoxya Hallmann, 1917, erected for south-east Australian Higginsia lunata Carter, 1885, was assigned to the synonymy of Higginsia Higgin, 1877 (Porifera: Halichondrida) by the latest revision of the taxonomy of the Porifera (Hooper & van Soest, 2002). The discovery of an undescribed species living in the bathyal reefs of the North Atlantic Rockall Bank with characters closely similar to H. lunata justifies the resurrection of Desmoxya . Accordingly the North Atlantic material is described as a new species in Desmoxya , viz. D. pelagiae sp. nov. The new species differs from its Australian congener in habit (smooth thin crusts vs massive with digitiform processes) and spicule sizes (smaller megascleres, twice-sized acanthoxeas and single vs two sizes of trichodragmas). A probable third member of this genus is Higginsia anfractuosa Hooper & Lévi, 1993, described from shallow-water reefs near New Caledonia. For reasons of priority the name of the family to which Higginsia and Desmoxya are assigned needs to be changed from Desmoxyidae Hallmann, 1917 to Heteroxyidae Dendy, 1905, as this senior synonym was overlooked when Heteroxya was assigned to the same family as Higginsia and Desmoxya in Hooper & van Soest, 2002.
Publisher: American Chemical Society (ACS)
Date: 23-04-2014
DOI: 10.1021/NP500119E
Abstract: Mass-directed fractionation of an extract from the Australian marine sponge Aplysinella sp., from the Great Barrier Reef, resulted in the isolation of four new bromotyrosine derivatives, aplysinellamides A-C (1-3) and aplysamine-1-N-oxide (4), along with six known compounds (5-10). The structure elucidation of compounds 1-4 was based on their 1D and 2D NMR and MS spectroscopic data. Aplysamine-1 (6) increased the apolipoprotein E secretion from human CCF-STTG1 astrocytoma cells by 2-fold at the concentration of 30 μM.
Publisher: American Chemical Society (ACS)
Date: 24-06-2008
DOI: 10.1021/NP800212G
Abstract: Three new diterpene alkaloids, agelasine J (3), agelasine K (4), and agelasine L (5), were isolated from the marine sponge Agelas cf. mauritiana collected in the Solomon Islands. The structures of these compounds were elucidated by physical data analyses. They displayed in vitro antimalarial activity against Plasmodium falciparum.
Publisher: Georg Thieme Verlag KG
Date: 22-03-2016
Abstract: Sponges are a useful source of bioactive natural products. Members of the family Mycalidae, in particular, have provided a variety of chemical structures including alkaloids, polyketides, terpene endoperoxides, peptides, and lipids. This review highlights the compounds isolated from Mycalid sponges and their associated biological activities. A erse group of 190 compounds have been reported from over 40 specimens contained in 49 references. Over half of the studies have reported on the biological activities for the compounds isolated. The polyketides, in particular the macrolides, displayed potent cytotoxic activities (< 1 µM), and the alkaloids, in particular the 2,5-disubstituted pyrrole derivatives, were associated with moderate cytotoxic activities (1-20 µM). The pyrrole alkaloids and the cyclic peroxides appear to be phylogenetically restricted to sponges and thus might prove useful when applied to sponge taxonomy. The observed ersity of chemical structures suggests this family makes a good target for targeted biodiscovery projects.
Publisher: Elsevier BV
Date: 11-1997
Publisher: American Chemical Society (ACS)
Date: 03-2008
DOI: 10.1021/NP700738K
Publisher: Wiley
Date: 08-2012
Abstract: Four s les of Suberea ianthelliformis were investigated and furnished five new and 13 known brominated tyrosine-derived compounds. Two of the new compounds were identified as araplysillin N20-formamide and its N-oxide derivative. Three other new compounds, araplysillins IV, V, and VI, were isolated and identified as analogs of araplysillin II. Most of these compounds exhibit moderate inhibitory activities against chloroquine-resistant and -sensitive strains of Plasmodium falciparum, and were investigated for their PFTase inhibitory properties. The chemical content of the investigated sponges is correlated with their molecular phylogeny.
Publisher: American Chemical Society (ACS)
Date: 06-08-2002
DOI: 10.1021/NP020228V
Abstract: Four beta-carbolines, plakortamines A-D, two cyclic peroxides, epiplakinic acids G and H, and two related gamma-lactones, (2S,4R)- and (2R,4R)-2,4-dimethyl-4-hydroxy-16-phenylhexadecanoic acid 1,4-lactones, were isolated from the deep-water sponge Plakortis nigra from Palau. The structures of the eight new metabolites were elucidated by interpretation of spectroscopic data. Most of the metabolites inhibited the HCT-116 human colon tumor cell line.
Publisher: CSIRO Publishing
Date: 2002
DOI: 10.1071/IS02015
Location: No location found
Location: Australia
Location: United Kingdom of Great Britain and Northern Ireland
Start Date: 2003
End Date: 2007
Funder: Cooperative Research Centres, Australian Government Department of Industry
View Funded ActivityStart Date: 2003
End Date: 2006
Funder: Cooperative Research Centres, Australian Government Department of Industry
View Funded ActivityStart Date: 2007
End Date: 2010
Funder: Australian Research Council
View Funded ActivityStart Date: 2005
End Date: 2008
Funder: European Commission
View Funded ActivityStart Date: 2008
End Date: 2010
Funder: Alfred P. Sloan Foundation
View Funded ActivityStart Date: 2008
End Date: 2011
Funder: Australian Research Council
View Funded ActivityStart Date: 2008
End Date: 2013
Funder: National Science Foundation
View Funded ActivityStart Date: 2008
End Date: 2010
Funder: National Institutes of Health
View Funded ActivityStart Date: 2006
End Date: 2008
Funder: Australian Biological Resources Study
View Funded ActivityStart Date: 2000
End Date: 2002
Funder: Australian Biological Resources Study
View Funded ActivityStart Date: 2009
End Date: 2012
Funder: Australian Biological Resources Study
View Funded ActivityStart Date: 2008
End Date: 2011
Funder: Australian Biological Resources Study
View Funded ActivityStart Date: 2014
End Date: 2016
Funder: Australian Biological Resources Study
View Funded ActivityStart Date: 2010
End Date: 2013
Funder: Australian Biological Resources Study
View Funded ActivityStart Date: 2016
End Date: 2019
Funder: Australian Biological Resources Study
View Funded ActivityStart Date: 2015
End Date: 2018
Funder: Australian Research Council
View Funded ActivityStart Date: 03-2008
End Date: 07-2011
Amount: $76,881.00
Funder: Australian Research Council
View Funded ActivityStart Date: 12-2015
End Date: 07-2018
Amount: $174,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 09-2007
End Date: 12-2011
Amount: $1,093,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 12-2003
End Date: 12-2004
Amount: $10,000.00
Funder: Australian Research Council
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