ORCID Profile
0000-0001-8286-5574
Current Organisation
University of California Riverside
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Publisher: Springer Science and Business Media LLC
Date: 11-03-2022
DOI: 10.1038/S41467-022-28981-7
Abstract: The RAP (RNA-binding domain abundant in Apicomplexans) protein family has been identified in various organisms. Despite expansion of this protein family in apicomplexan parasites, their main biological functions remain unknown. In this study, we use inducible knockdown studies in the human malaria parasite, Plasmodium falciparum , to show that two RAP proteins, PF3D7_0105200 ( Pf RAP01) and PF3D7_1470600 ( Pf RAP21), are essential for parasite survival and localize to the mitochondrion. Using transcriptomics, metabolomics, and proteomics profiling experiments, we further demonstrate that these RAP proteins are involved in mitochondrial RNA metabolism. Using high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (eCLIP-seq), we validate that Pf RAP01 and Pf RAP21 are true RNA-binding proteins and interact specifically with mitochondrial rRNAs. Finally, mitochondrial enrichment experiments followed by deep sequencing of small RNAs demonstrate that Pf RAP21 controls mitochondrial rRNA expression. Collectively, our results establish the role of these RAP proteins in mitoribosome activity and contribute to further understanding this protein family in malaria parasites.
Publisher: Public Library of Science (PLoS)
Date: 16-04-2014
Location: United States of America
No related grants have been discovered for Anil Bhatia.