ORCID Profile
0000-0002-4364-0521
Current Organisation
CSIRO Black Mountain Laboratories
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Publisher: Oxford University Press (OUP)
Date: 11-02-2016
DOI: 10.1093/JXB/ERW035
Publisher: Springer Science and Business Media LLC
Date: 02-07-2009
Publisher: Cold Spring Harbor Laboratory
Date: 29-08-2020
DOI: 10.1101/2020.08.27.271205
Abstract: Phosphorus (P) is an essential plant nutrient and regular applications are essential in most farming systems to maintain high yields. Yet the P fertilizers applied to crops and pastures are derived from non-renewable resources. It is therefore important to find agronomic and genetic strategies for using this resource efficiently, especially since only a proportion of the applied P is absorbed by crops. The aim of this study was to identify Quantitative Trait Loci (QTL) for P use efficiency (PUE) in wheat using a Multiparent Advanced Generation InterCross (MAGIC) population grown in the field. The 357 genotypes were arranged in paired plots with and without P fertilization. Yield and biomass were measured and PUE was calculated as either the performance of the genotype relative to the average response to fertilization, or the performance of the genotype relative to the average resilience in the absence of fertilization. Five trials were conducted over three years in Australia at three sites with contrasting clay and sandy soil types. Genotypic variation for response and resilience were identified in all trials with moderate to strong correlation with productivity with and without P between trials. Multiparent Whole Genome Average Interval Mapping (WGAIM) QTL analyses were conducted on the four traits (Biomass / Yield × P Response / Resilience) across the five trials and identified 130 QTL in total. QTL within 10 cM of each other were clustered into 56 groups that were likely to represent identical or linked loci. Of the clusters 27 (48%) contained only a single QTL but 17 (31%) contained 3 or more in different trials or traits. This suggests multiple biological mechanisms driving PUE in different environments. Eight of the 56 groups collocated with QTL for seedling root hair length identified in the same MAGIC population in an earlier study. Identification of genetic loci for phosphorus use efficiency in a multigenic population of Australian wheats grown on contrasting soils.
Publisher: Springer Science and Business Media LLC
Date: 27-03-2008
Publisher: Oxford University Press (OUP)
Date: 28-08-2015
DOI: 10.1093/JXB/ERV403
Publisher: Springer Science and Business Media LLC
Date: 27-03-2010
Publisher: Wiley
Date: 24-02-2014
DOI: 10.1111/PPL.12150
Publisher: CSIRO Publishing
Date: 23-08-2022
DOI: 10.1071/SR22012
Abstract: Context Analytical procedures and technologies for soil analyses can be prohibitively expensive for small laboratories and researchers in developing countries. There is a need for low cost and high-throughput methods for assaying pH, extractable aluminium and phosphorus when conducting field trials on acid soils. Aims We investigated methods to develop rapid yet inexpensive colorimetric assays for the assay of pH, extractable aluminium and Colwell phosphorus in soil extracts. Methods We developed a colorimetric method to measure soil pH enabling pH to be quantified in a high-throughput assay. Similarly, two existing methods for extractable aluminium and Colwell P were modified for high throughput assays also using microtiter plates. Key results All three methods yielded linear relationships when using absorbance to quantify the parameters with the high throughput methods. Furthermore, there was a strong correlation between pH values of the soil s les obtained with the colorimetric assay and pH values measured with a glass electrode. Conclusions We demonstrated that the rapid assays for all three methods can be implemented to characterise field sites through the mapping of distributions for extractable Al, Colwell P and pH. Implications The high-throughput methods described here will be useful for researchers who conduct field trials to map variations in soil pH, soluble Al and Colwell P. Although the focus of the current work was on acid soils, the colorimetric pH and Colwell P methods can also be applied to non-acid soils.
No related grants have been discovered for Chandrakumara Weligama.