ORCID Profile
0000-0002-9427-2028
Current Organisation
Universiti Putra Malaysia
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Publisher: Elsevier BV
Date: 15-12-2008
Publisher: Friends Science Publishers
Date: 03-2018
Publisher: Springer Science and Business Media LLC
Date: 12-2015
Publisher: Inter-Research Science Center
Date: 09-08-2017
DOI: 10.3354/AB00678
Publisher: Hindawi Limited
Date: 21-12-2019
DOI: 10.1155/2019/3480569
Abstract: Epigallocatechin-3-gallate (EGCG) is the most abundant bioactive polyphenolic compound among the green tea constituents and has been identified as a potential anticancer agent in colorectal cancer (CRC) studies. This study was aimed to determine the mechanism of actions of EGCG when targeting the endoplasmic reticulum (ER) stress pathway in CRC. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay was performed on HT-29 cell line and normal cell line (3T3) to determine the EGCG toxicity. Next, western blot was done to observe the expression of the related proteins for the ER stress pathway. The Caspase 3/7 assay was performed to determine the apoptosis induced by EGCG. The results demonstrated that EGCG treatment was toxic to the HT-29 cell line. EGCG induced ER stress in HT-29 by upregulating immunoglobulin-binding (BiP), PKR-like endoplasmic reticulum kinase (PERK), phosphorylation of eukaryotic initiation factor 2 alpha subunit (eIF2 α ), activating transcription 4 (ATF4), and inositol-requiring kinase 1 alpha (IRE1 α ). Apoptosis was induced in HT-29 cells after the EGCG treatment, as shown by the Caspase 3/7 activity. This study indicates that green tea EGCG has the potential to inhibit colorectal cancer cells through the induction of ER stress.
Publisher: Springer Science and Business Media LLC
Date: 24-10-2017
Publisher: Emerald
Date: 02-2013
DOI: 10.1108/00346651311295897
Abstract: Flaxseed (FL) is a nutrient rich seed but contains cyanogenic glycosides, which can release hydrogen cyanide (HCN) into the body after consumption. This study aims to determine the cyanogenic content of raw and heated (170°C, 15 min) flaxseed as well as its effect on the blood thiocyanate (SCN − ) concentration, a derivate of HCN, in rats. Changes in liver and kidney enzymes level of blood are further studied. Rats were assigned into groups of control, 10 percent, 20 percent and 30 percent of either raw or heated FL, in basal diet, for duration of 30 days. Cyanogenic content of FL analyzed using picrate method and blood thiocyanate concentration analyzed using high performance liquid chromatography. Raw ground flaxseed had 251.14±9.11 mg HCN/kg food, which significantly reduced by 8.5 percent after heating. Generally heated FL groups showed lower (but not significant) blood thiocyanate compared to raw FL groups. Blood SCN elevated by increasing the dosage of flaxseed. However, only in 30 percent raw FL group the difference was significant ( p .05). Liver and kidney enzymes were not changed significantly in all groups ( p .05). This study suggests that consumption of flaxseed in high dosage (20 percent to 30 percent) is better to be in heated form to result in lower blood thiocyanate concentration. This study may provide better understanding for safer usage of flaxseed in animals and even human interventional studies and may be applicable to flaxseed consuming food industries.
Publisher: Informa UK Limited
Date: 13-03-2013
DOI: 10.3109/09637486.2013.775227
Abstract: Evidences from several studies suggest that probiotics affect glucose homeostasis. This paper reviews the results of animal and human studies on the role of probiotics in modulating glucose homeostasis. A systematic literature search using multiple databases was conducted without time limitation. Primary outcomes evaluated were parameters related to glucose homeostasis. Secondary outcomes were inflammatory markers, lipid profile, body weight, and energy intake. A total of 17 animal studies and four human studies were identified. Among these, 16 animal studies and three human studies had documented significant improvements in at least one glucose homeostasis related parameter. Inflammatory markers and lipid profile were significantly improved in the animal model, while data from human studies were controversial. Changes in body weight and energy intake that could be due to probiotics supplementation were also inconclusive. Well-designed placebo-controlled clinical trials with validated outcome variables are needed to determine the effect of probiotics on glucose homeostasis.
Publisher: Wiley
Date: 14-03-2008
DOI: 10.1002/JSFA.3186
Publisher: Springer Science and Business Media LLC
Date: 17-03-2017
DOI: 10.1007/S00394-016-1199-8
Abstract: Evidence of a possible connection between gut microbiota and several physiological processes linked to type 2 diabetes is increasing. However, the effect of multi-strain probiotics in people with type 2 diabetes remains unclear. This study investigated the effect of multi-strain microbial cell preparation-also refers to multi-strain probiotics-on glycemic control and other diabetes-related outcomes in people with type 2 diabetes. A randomized, double-blind, parallel-group, controlled clinical trial. Diabetes clinic of a teaching hospital in Kuala Lumpur, Malaysia. A total of 136 participants with type 2 diabetes, aged 30-70 years, were recruited and randomly assigned to receive either probiotics (n = 68) or placebo (n = 68) for 12 weeks. Primary outcomes were glycemic control-related parameters, and secondary outcomes were anthropomorphic variables, lipid profile, blood pressure and high-sensitivity C-reactive protein. The Lactobacillus and Bifidobacterium quantities were measured before and after intervention as an indicator of successful passage of the supplement through gastrointestinal tract. Intention-to-treat (ITT) analysis was performed on all participants, while per-protocol (PP) analysis was performed on those participants who had successfully completed the trial with good compliance rate. With respect to primary outcomes, glycated hemoglobin decreased by 0.14 % in the probiotics and increased by 0.02 % in the placebo group in PP analysis (p < 0.05, small effect size of 0.050), while these changes were not significant in ITT analysis. Fasting insulin increased by 1.8 µU/mL in placebo group and decreased by 2.9 µU/mL in probiotics group in PP analysis. These changes were significant between groups at both analyses (p < 0.05, medium effect size of 0.062 in PP analysis and small effect size of 0.033 in ITT analysis). Secondary outcomes did not change significantly. Probiotics successfully passed through the gastrointestinal tract. Probiotics modestly improved HbA1c and fasting insulin in people with type 2 diabetes.
Publisher: Elsevier BV
Date: 08-2009
Publisher: Elsevier BV
Date: 2006
Publisher: Springer Science and Business Media LLC
Date: 17-08-2015
Publisher: Emerald
Date: 04-2004
DOI: 10.1108/00346650410529005
Abstract: This study was aimed to estimate and compare the contents of protein and amino acids in raw, boiled and fried fishes of Indian mackerel “kembong” ( Rastrelliger kanagurta ), sardine ( Sardina pilchardus ), red tilapia ( Oreochromis mossambicusx ) and black tilapia ( Oreochromis mossambicus ). Protein contents of raw mackerel, sardine, red and black tilapia were 8.1±0.0, 8.4±0.1, 9.6±0.4 and 9.0±0.0 percent, respectively. In a boiled fish, the protein contents were 7.9±0.1, 7.7±0.0, 7.5±0.1 and 8.9±0.1 percent, respectively, and for a fried fish the values were 8.6±0.5, 8.9±0.1, 9.1±0.2 and 8.4±0.0 percent, respectively. It was found that there was a significant difference ( p .01) in the protein content of the raw fish compared to the heat‐treated ones for all the fishes. The study detected 17 components of essential amino acids (lysine, histidine, threonine, valine, methionine, leucine, isoleucine and phenylalanine) and non‐essential amino acids (arginine, aspartic acid, serine, glutamic acid, proline, glycine, alanine, cystein and tyrosine) in all the fishes. There was no significant difference in amino acids content among the boiled and fried fishes. In conclusion, heat treatment for five minutes in boiling water (100°C) and frying for three minutes in palm oil (160°C) did not alter the quality of protein in all the fishes studied.
Publisher: Hindawi Limited
Date: 21-03-2020
DOI: 10.1155/2020/7958041
Abstract: In many studies, green tea epigallocatechin-3-gallate (EGCG) has already shown its therapeutic effects in colorectal cancer cells (CRC). However, its mechanism of actions in CRC is poorly elucidated. Hence, this study attempts to elucidate the mechanism of actions of green tea ECGG via iron chelation activity in CRC. In order to investigate this property, HT-29 cell lines (CRC) were treated with EGCG for 24 h, 48 h, and 72 h. From western blot analysis, EGCG had upregulated transferrin receptor (TfR) protein and downregulated Ferritin-H (FtH) protein indicating that iron chelation activity has occurred in CRC. Meanwhile, the molecular docking study demonstrated that EGCG is able to strongly interact the ferritin protein with a high binding affinity (−7.3 kcal/mol) via strong hydrogen bindings to glutamic acid 64 and lysine 71 two moderate hydrogen bindings to asparagine 74 and a hydrophobic interaction to the hydrophobic pocket of lysine 71. The strong interaction predicted between EGCG to ferritin may lead to inhibition of ferritin by EGCG, thus supporting the downregulation of FtH observed in in vitro studies. Molecular docking study of TfR to EGCG cannot be modulated based on the in vitro results. In conclusion, EGCG possesses iron chelator property in CRC and this potential could be further exploited for CRC treatment.
No related grants have been discovered for Amin Ismail.