ORCID Profile
0000-0001-6070-5035
Current Organisation
University of Queensland
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In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Nanotechnology | Nanomaterials | Nanotechnology | Colloid And Surface Chemistry | Chemical Engineering | Crop and Pasture Protection (Pests, Diseases and Weeds) | Biomedical Engineering not elsewhere classified | Nanobiotechnology | Biological And Medical Chemistry | Powder and Particle Technology | Crop and Pasture Production | Plant Nutrition | Chemical Engineering Not Elsewhere Classified | Nanotoxicology, Health and Safety | Nanomedicine | Plant Cell and Molecular Biology | Medical Biotechnology | Chemical Engineering not elsewhere classified | Horticultural Crop Protection (Pests, Diseases and Weeds) | Fluidisation and Fluid Mechanics | Gene Therapy | Nonlinear Optics and Spectroscopy |
Nervous system and disorders | Expanding Knowledge in the Biological Sciences | Inorganic industrial chemicals | Clay products | Preventive Medicine | Skin and Related Disorders | Substance Abuse | Nervous System and Disorders | Expanding Knowledge in Technology | Beneficiation or dressing of non-metallic minerals (incl. diamonds) | Control of Plant Pests, Diseases and Exotic Species in Farmland, Arable Cropland and Permanent Cropland Environments | Cardiovascular System and Diseases | Energy minerals not elsewhere classified | Expanding Knowledge in Engineering | Human Pharmaceutical Products not elsewhere classified | Expanding Knowledge in the Agricultural and Veterinary Sciences | Cancer and Related Disorders | Prevention—biologicals (e.g. vaccines) | Treatments (e.g. chemicals, antibiotics) | Crop and animal protection chemicals | Chemical fertilisers | Clay Products
Publisher: American Chemical Society (ACS)
Date: 23-12-2010
DOI: 10.1021/JP905347X
Publisher: Elsevier BV
Date: 12-2011
Publisher: American Chemical Society (ACS)
Date: 06-2010
DOI: 10.1021/JP103115Q
Publisher: Elsevier BV
Date: 05-2011
DOI: 10.1016/J.JHAZMAT.2011.02.078
Abstract: Triphosphate (TPP) is an important form of phosphate pollutants while its removal investigation has been just started now. This research examined the removal of triphosphate using Mg(2-x)Ca(x)FeCl-LDH (x = 0-2) as absorbents. We found that the removal of triphosphate over Mg(2)FeCl-LDH mainly underwent the surface adsorption and the near-edge intercalation, with the practical removal amount (9-11 mg(P)/g) corresponding to 10-15% of the theoretical one. In contrast, Ca(2)FeCl-LDH removed a higher amount of triphosphate (56.4 mg(P)/g). The comprehensive analysis of the triphosphate-uptake products with XRD/XPS/FTIR reveals that Ca(2)FeCl-LDH dissolves first and then released Ca(2+) ions react with triphosphate (TPP) to form insoluble Ca-TPP precipitate. Combination of these two different removal mechanisms enables Mg(0.5)Ca(1.5)FeCl-LDH to take up 84.2mg(P)/g from aqueous solution under similar conditions.
Publisher: Elsevier BV
Date: 03-2010
Publisher: Elsevier BV
Date: 03-2010
Publisher: Elsevier BV
Date: 12-2018
DOI: 10.1016/J.JCONREL.2018.11.008
Abstract: Currently, there are few strategies for controlling pathogenic bacteria, especially the pathotypes of Escherichia coli which are an emerging threat to public health worldwide. Here, multivalent vaccine formulations are reported for control of pathogenic E. coli. The formulations utilised clay nanoparticles, either layered double hydroxides (LDH) or hectorite (HEC), to complex with a cocktail of three recombinant antigens, intimin β (IB), proprietary antigen 1 (PAg1) and proprietary antigen 2 (PAg2). Acting as nano-adjuvants, LDH and HEC were able to stimulate strong, durable and balanced immune responses in mice. Moreover, LDH-IB-PAg1-PAg2 and HEC-IB-PAg1-PAg2 immunised mice developed potent mucosal immune responses and efficiently prevented adherence of enterohemorrhagic E. coli serotype O26 to mammalian cells. Notably, the multi-faceted immune responses elicited by the clay nanoparticle formulations were significantly higher than those induced by a QuilA formulation, without antigenic competition observed for the first time. The results of this study suggest that LDH and HEC offer considerable promise as effective multivalent vaccine carriers against important pathogens such as enteropathogenic E. coli.
Publisher: MDPI
Date: 07-04-2020
Publisher: Elsevier BV
Date: 09-2011
DOI: 10.1016/J.JHAZMAT.2011.06.091
Abstract: Flash fixation of heavy metals from electroplating wastewater (EPW) and pickling waste liquor (PWL) into ferrite lattice can be investigated by microwave hydrothermal process. The toxicity of wastewater may be reduced by the redox reaction between Cr(VI) in electroplating wastewater and Fe(II) in pickling waste liquor. Box-Behnken design (BBD) experiment gives optimal process condition of ferrite formation as follows: wastewater volume ratio (V(PWL):V(EPW) = 0.20), pH value 11 and retention time 15 min, on which formed ferrite has a soft magnetic property with high saturation magnetization (M(s)) 47.4 emu/g. The rapid ferrite process has lower activation energy 7.01 kJ/mol according to grain growth kinetics. Concerning the environmental and economy, we introduced a new and interesting method for water remediation simultaneously synthesizing ferrite by using microwave mediated hydrothermal processes.
Publisher: Elsevier BV
Date: 2012
Publisher: Informa Healthcare
Date: 17-08-2009
DOI: 10.1517/17425240903130585
Abstract: Layered double hydroxides (LDHs) have been known for many decades as catalyst and ceramic precursors, traps for anionic pollutants, catalysts and additives for polymers, but their successful synthesis on the nanometer scale a few years ago opened up a whole new field for their application in nanomedicine. The delivery of drugs and other therapeutic/bioactive molecules (e.g., peptides, proteins, nucleic acids) to mammalian cells is an area of research that is of tremendous importance to medicine and provides manifold applications for any new developments in the area of nanotechnology. Among the many different nanoparticles that have been shown to facilitate gene and/or drug delivery, LDH nanoparticles have attracted particular attention owing to their many desirable properties. This review aims to report recent progress in gene and drug delivery using LDH nanoparticles. It summarizes the advantages and disadvantages of using LDH nanoparticles as carriers for nucleic acids and drugs against the general background of bottlenecks that are encountered by cellular delivery systems. It describes further the models that have been proposed for the internalization of LDH nanoparticles into cells so far and discusses the intracellular fate of the particles and their cargo. The authors offer some remarks on how this field of research will progress in the near future and which challenges need to be overcome before LDH nanoparticles can be used in a clinical setting.
Publisher: Elsevier BV
Date: 10-2011
DOI: 10.1016/J.BIOMATERIALS.2011.05.083
Abstract: This paper reports a clear elucidation of the pathway for the cellular delivery of layered double hydroxide (LDH) nanoparticles intercalated with anti-restenotic low molecular weight heparin (LMWH). Cellular uptake of LMWH-LDH conjugates into cultured rat vascular smooth muscle cells (SMCs) measured via flow cytometry was more than ten times greater than that of LMWH alone. Confocal and transmission electron microscopy showed LMWH-LDH conjugates taken up by endosomes, then released into the cytoplasm. We propose that LMWH-LDH is taken up via a unique 'modified endocytic' pathway, whereby the conjugate is internalized by SMCs in early endosomes, sorted in late endosomes, and quickly released from late endosomes/lysosomes, avoiding degradation. Treatment of cells with LMWH-LDH conjugates suppressed the activation of ERK1/2 in response to foetal calf serum (FCS) for up to 24h, unlike unconjugated LMWH which had no significant effect at 24h. Improved understanding of the intracellular pathway of LMWH-LDH nanohybrids in SMC will allow for refinement of design for LDH nanomedicine applications.
Publisher: Elsevier BV
Date: 15-04-2010
DOI: 10.1016/J.JHAZMAT.2009.11.012
Abstract: This research has demonstrated that the Friedel phase, e.g. a chloride-containing hydrocalumite (Ca(2)Al(OH)(6)Cl(H(2)O)(2) x mH(2)O), can rapidly adsorb large amounts of SeO(4)(2-) (up to 1.37 mmol/g). SeO(4)(2-) is removed via anionic exchange, as evidenced by the expansion of the d-spacing from 0.78 nm of Cl-hydrocalumite to 0.97-0.98 nm of SeO(4)-hydrocalumite. The newly formed SeO(4)-adsorbed hydrocalumite is stable in water at pH 4-13, indicating the strong fixation of selenate within the phase. In contrast, intercalated selenate in the Freidel phase can be recovered by desorbing in the NaCl solution, which can also regenerate and recycle the used adsorbent. The findings in this research strongly suggest that the Friedel phase is a new, environmentally friendly and cost-effective adsorbent to adsorb selenate from wastewater streams and dilute solutions.
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2TB00021K
Abstract: We report the development of a photochromic nanoprobe that can be activated by remote light for sulphite fluorescence detection and imaging.
Publisher: Elsevier BV
Date: 09-2014
Publisher: American Chemical Society (ACS)
Date: 12-10-2021
Abstract: Oral vaccine has attracted much interest, as it can stimulate both mucosal and systemic immunity with noninvasive and good patient compliance. However, the oral vaccine efficiency is strongly constrained by the low absorption of antigens in the small intestine due to the mucosal barriers. Physicochemical characteristics of nanoparticles (NPs) have strong effects on antigen mucosal penetration, helping to improve immune response. However, surface functions of NPs on mucosal transportation have not been clearly understood. In this work, we elaborately investigated how the surface characteristics of mucoadhesive chitosan and its derivant act on oral antigen absorption and immune response. Core-shell chitosan- and
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C0CY00018C
Publisher: American Chemical Society (ACS)
Date: 27-10-2000
DOI: 10.1021/CM000371E
Publisher: Elsevier BV
Date: 05-2011
DOI: 10.1016/J.BIORTECH.2010.10.061
Abstract: This research for the first time investigated hydrogen production from the fresh leachate originated from municipal solid wastes. We found that fermentation of the leachate generated H(2) and was very much enhanced in the presence of extra phosphate in the batch reactor. The continuous expanded granular sludge bed (EGSB) reactor started to generate H(2) at day 20 and continued to 176 days with 120 mg/l of extra phosphate present. The highest chemical oxygen demand (COD) removal efficiency (66.9%) was achieved at liquid up-flow velocity of 3.7 m/h and hydraulic retention time of 12h. Under proposed optimal operation conditions, the mean H(2) production rate reached up to 2155 ml/(l day). We also found that over 80% liquid metabolites were acetic acid and ethanol, suggesting the ethanol-type fermentation was dominant in the bioreactor. These findings indicate that the fresh leachate can be used as the source for continuous hydrogen production.
Publisher: Wiley
Date: 22-12-2022
Abstract: Novel sonosensitizers with intrinsic characteristics for tumor diagnosis, efficient therapy, and tumor microenvironment regulation are appealing in current sonodynamic therapy. Herein, a manganese (Mn)‐layered double hydroxide‐based defect‐rich nanoplatform is presented as a new type of sono‐chemo sensitizer, which allows ultrasound to efficiently trigger reactive oxygen species generation for enhanced sono/chemo‐dynamic therapy. Moreover, such a nanoplatform is able to relieve tumor hypoxia and achieve augmented singlet oxygen production via catalyzing endogenous H 2 O 2 into O 2 . On top of these actions, the released Mn 2+ ions and immune‐modulating agent significantly intensify immune activation and reverse the immunosuppressive tumor microenvironment to the immunocompetent one. Consequently, this nanoplatform exhibits excellent anti‐tumor efficacy and effectively suppresses both primary and distant tumor growth, demonstrating a new strategy to functionalize nanoparticles as sono‐chemo sensitizers for synergistic combination cancer therapy.
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C4CC07885C
Abstract: Polymeric monofunctional nanoparticles with exactly one functional group on the surface of each nanoparticle are prepared.
Publisher: Elsevier BV
Date: 12-2014
DOI: 10.1016/J.JCIS.2014.08.043
Abstract: Nitrate reduction by zero-valent iron (Fe(0)) powder always works well only at controlled pH lower than 4 due to the formation of iron (hydr)oxides on its surface. Fe(0) powder combined with activated carbon (AC), i.e., Fe(0)/AC micro-electrolysis system, was first introduced to enhance nitrate reduction in aqueous solution. Comparative study was carried out to investigate nitrate reduction by Fe(0)/AC system and Fe(0) under near-neutral conditions, showing that the Fe(0)/AC system successfully reduced nitrate even at initial pH 6 with the reduction efficiency of up to 73%, whereas for Fe(0) only ∼10%. The effect of Fe(0) to AC mass ratio on nitrate reduction efficiency was examined. Easier nitrate reduction was achieved with more contact between Fe(0) and AC as the result of decreasing Fe(0) to AC mass ratio. Ferrous ion and oxidation-reduction potential were measured to understand the mechanism of enhanced nitrate reduction by Fe(0)/AC micro-electrolysis. The results suggest that a relative potential difference drives much more electrons from Fe(0) to AC, thus generating adsorbed atomic hydrogen which makes it possible for nitrate to be reduced at near-neural pH. Fe(0)/AC micro-electrolysis thus presents a great potential for practical application in nitrate wastewater treatment without excessive pH adjustment.
Publisher: American Chemical Society (ACS)
Date: 23-09-2019
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C7NR08644J
Abstract: An optimized dual-target lipid-coated calcium phosphate-based nanoplatform efficiently delivers the drug/gene to triple-negative human breast tumor cells in vitro and in vivo .
Publisher: Elsevier BV
Date: 12-2023
Publisher: Elsevier BV
Date: 05-2010
Publisher: IOP Publishing
Date: 09-10-2007
Publisher: American Chemical Society (ACS)
Date: 27-02-2017
Abstract: A novel layered double hydroxide (LDH) nanoparticle/thermogel composite drug delivery system (DDS) for sustained release of brimonidine (Bri) has been designed, prepared, and characterized in this study for treatment of severe glaucoma. Brimonidine is first loaded onto LDH (Bri@LDH) nanoparticles, which are then dispersed in the thermogel consisting of plenty of micelles based on poly(dl-lactic acid-co-coglycolic acid)-polyethylene glycol-poly(dl-lactic acid-co-coglycolic acid) (PLGA-PEG-PLGA) copolymer. The Bri@LDH/Thermogel DDS containing 125.0 μg/g of brimonidine has been found to sustainably release the drug for up to 144 h, significantly extending the drug release period compared to that from Bri@LDH nanoparticles. The Bri@LDH/Thermogel DDS is not cytotoxic to human corneal epithelial cells and shows good biocompatibility. In vivo drug release from the special contact lens made of Bri@LDH/Thermogel DDS has been sustained for at least 7 days, which more effectively modulates the relief of intraocular pressure (IOP). Thus, the Bri@LDH/Thermogel DDS is a promising drug delivery alternative that can be used for treatment of severe glaucoma.
Publisher: Springer Science and Business Media LLC
Date: 05-2014
Publisher: Elsevier BV
Date: 15-01-2008
Publisher: American Chemical Society (ACS)
Date: 07-08-2023
Publisher: Elsevier BV
Date: 03-2010
Publisher: Informa UK Limited
Date: 07-2014
DOI: 10.2147/IJN.S61633
Publisher: American Chemical Society (ACS)
Date: 22-02-2000
DOI: 10.1021/CM990355L
Publisher: American Chemical Society (ACS)
Date: 12-10-2001
DOI: 10.1021/CM010222B
Publisher: Elsevier BV
Date: 09-2009
Publisher: American Chemical Society (ACS)
Date: 09-06-2017
Abstract: Active targeting of nanoparticles (NPs) for cancer treatment has attracted increasing interest in the past decades. Various ligand modification strategies have been used to enhance the targeting of NPs to the tumor site. However, how to reproducibly fabricate erse targeting NPs with narrowly changeable biophysiochemical properties remains as a major challenge. In this study, layered double hydroxide (LDH) NPs were modified as a target delivery system. Two brain tumor targeting ligands, i.e., angiopep-2 and rabies virus glycoprotein, were conjugated to the LDH NPs via an intermatrix protein moiety, bovine serum albumin (BSA), simultaneously endowing the LDHs with excellent colloidal stability and targeting capability. The ligands were first covalently linked with BSA through the heterobifunctional cross-linker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate. Then, the ligand-linked BSA and pristine BSA were together coated onto the surface of LDHs through electrostatic interaction, followed by cross-linking with the cross-linker glutaraldehyde to immobilize these BSAs on the LDH surface. In this way, we are able to readily prepare colloidally stabilized tumor-targeted LDH NPs. The targeting efficacy of the ligand-conjugated LDH delivery system has been evidenced in the uptake by two neutral cells (U87 and N2a) compared to unmodified LDHs. This new approach provides a promising strategy for rational design and preparation of target nanoparticles as a selective and effective therapeutic treatment for brain tumors.
Publisher: Elsevier BV
Date: 06-2009
Publisher: Wiley
Date: 25-05-2022
DOI: 10.1002/EXP.20210181
Abstract: Nanoparticle‐based drug delivery has become one of the most popular approaches for maximising drug therapeutic potentials. With the notable improvements, a greater challenge hinges on the formulation of gasotransmitters with unique challenges that are not met in liquid and solid active ingredients. Gas molecules upon release from formulations for therapeutic purposes have not really been discussed extensively. Herein, we take a critical look at four key gasotransmitters, that is, carbon monoxide (CO), nitric oxide (NO), hydrogen sulphide (H 2 S) and sulphur dioxide (SO 2 ), their possible modification into prodrugs known as gas‐releasing molecules (GRMs), and their release from GRMs. Different nanosystems and their mediatory roles for efficient shuttling, targeting and release of these therapeutic gases are also reviewed extensively. This review thoroughly looks at the erse ways in which these GRM prodrugs in delivery nanosystems are designed to respond to intrinsic and extrinsic stimuli for sustained release. In this review, we seek to provide a succinct summary for the development of therapeutic gases into potent prodrugs that can be adapted in nanomedicine for potential clinical use.
Publisher: Elsevier BV
Date: 07-2010
DOI: 10.1016/J.BIOMATERIALS.2010.03.050
Abstract: Surgical procedures to remove atherosclerotic lesions and restore blood flow also injure the artery wall, promoting vascular smooth muscle cell (SMC) phenotypic change, migration, proliferation, matrix production and ultimately, restenosis of the artery. Hence identification of effective anti-restenotic strategies is a high priority in cardiovascular research, and SMCs are a key target for intervention. This paper presents the in vitro study of layered double hydroxides (LDHs) as drug delivery system for an anti-restenotic drug (low molecular weight heparin, LMWH). The cytotoxicity tests showed that LDH itself had very limited toxicity at concentrations below 50 microg/mL over 6-day incubation. LDH nanoparticles loaded with LMWH (LMWH-LDHs) were prepared and tested on rat vascular SMCs. When conjugated to LDH particles, LMWH enhanced its ability to inhibit SMC proliferation and migration, with greater than above 60% reduction compared with the control (growth medium) over 3 or 7-day incubation. Cellular uptake studies showed that compared with LMWH alone, LMWH-LDH hybrids were internalized by SMCs more rapidly, and uptake was sustained over a longer time, possibly revealing the mechanisms underlying the enhanced biological function of LMWH-LDH. The results suggest the potential of LMWH-LDH as an efficient anti-restenotic drug for clinical application.
Publisher: Elsevier BV
Date: 02-2006
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C7TB02527K
Abstract: This review focuses on the biomedical applications of upconversion luminescence nanomaterials, including lanthanide-doped inorganic nanocrystals and TTA-based UCNPs.
Publisher: American Chemical Society (ACS)
Date: 06-06-2013
DOI: 10.1021/ES400553E
Abstract: This paper reports that recycled electroplating sludge is able to efficiently remove greenhouse gas sulfur hexafluoride (SF6). The removal process involves various reactions of SF6 with the recycled sludge. Remarkably, the sludge completely removed SF6 at a capacity of 1.10 mmol/g (SF6/sludge) at 600 °C. More importantly, the evolved gases were SO2, SiF4, and a limited amount of HF, with no toxic SOF4, SO2F2, or SF4 being detected. These generated gases can be readily captured and removed by NaOH solution. The reacted solids were further found to be various metal fluorides, thus revealing that SF6 removal takes place by reacting with various metal oxides and silicate in the sludge. Moreover, the kinetic investigation revealed that the SF6 reaction with the sludge is a first-order chemically controlled process. This research thus demonstrates that the waste electroplating sludge can be potentially used as an effective removal agent for one of the notorious greenhouse gases, SF6.
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1BM00941A
Abstract: A reliable approach is developed to formulate a targeted lipid-coated nanomedicine based on UCNP and co-loaded with CO-prodrug and vitamin E, which can convert NIR light to UV photon to release CO for synergistical cancer therapy with vitamin E.
Publisher: American Chemical Society (ACS)
Date: 14-12-2022
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1NJ01843D
Abstract: A manganese dioxide-based nanoprobe is developed for fluorescence detection and imaging of glutathione (GSH) in yeast cells and onion tissues.
Publisher: Elsevier BV
Date: 05-2008
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C3TB21760D
Abstract: The recent advances of ultra-small fluorescence inorganic nanoparticles including quantum dots, metal nanoclusters, carbon and graphene dots, up-conversion nanocrystals, and silicon nanoparticles have been comprehensively reviewed.
Publisher: American Chemical Society (ACS)
Date: 09-09-2019
Abstract: Cancer recurrence and metastasis are worldwide challenges but current bimodular strategies such as combined radiotherapy and chemotherapy (CTX), and photothermal therapy (PTT) and immunotherapy have succeeded only in some limited cases. Thus in the present study, a multifunctional nanomedicine has been rationally designed via elegantly integrating three FDA-approved therapeutics, that is, indocyanine green (for PTT), doxorubicin (for CTX), and CpG (for immunotherapy) into the structure of layered double hydroxide (LDH) nanoparticles, aiming to completely prevent the recurrence and metastasis of invasive breast cancer. This multifunctional hybrid nanomedicine has been demonstrated to eliminate the primary tumor and efficiently prevent tumor recurrence and lung metastasis through combined PTT/CTX and induction of specific and strong immune responses mediated by the hybrid nanomedicine in a 4T1 breast cancer mouse model. Furthermore, the promoted in situ immunity has significantly inhibited the growth of reinoculated distant tumors. Altogether, our multifunctional LDH-based nanomedicine has showed an excellent efficacy in invasive cancer treatment using much lower doses of three FDA-approved therapeutics, providing a preclinical/clinical alternative to cost-effectively treat invasive breast cancer.
Publisher: American Chemical Society (ACS)
Date: 17-08-2000
DOI: 10.1021/CM000053F
Publisher: Elsevier BV
Date: 06-2012
DOI: 10.1016/J.BIORTECH.2011.11.114
Abstract: The inhibitory effect of high-strength NH(3)-N on anaerobic biodegradation of landfill leachates in an EGSB bioreactor has been investigated. The research compared start-up performance of the reactor treating the landfill leachate with NH(3)-N in 242-1200 mg/l to that treating the compost leachate with NH(3)-N in 38-410 mg/l. The observations showed that the performance of the reactor treating the landfill leachate was only marginally worse than that treating the compost leachate at the mesophilic temperature when NH(3)-N concentration was under 1500 mg/l. We also noted that NH(3)-N at the concentration of 1500-3000 mg/l inhibited the biodegradation. The comparative biodegradation performance at the mesophilic and atmospheric temperature demonstrated that the maximal OLR of atmospheric digestion was only reduced to 44 kg COD/m(3)d. These findings indicate that landfill leachates with NH(3)-N less than 1500 mg/l could be efficiently treated in the EGSB bioreactor even under the atmospheric condition with methane generated.
Publisher: Elsevier BV
Date: 30-10-2009
DOI: 10.1016/J.JHAZMAT.2009.05.070
Abstract: Friedel's salt (3CaO x Al2O3 x CaCl2 x 10 H2O or Ca4Al2(OH)12Cl2(H2O)4) is a calcium aluminate hydrate formed by hydrating cement or concrete in seawater at a low cost. In the current study, we carefully examined the adsorption behaviors of Friedel's salt for Cr(VI) from aqueous solution at different concentrations and various initial pHs. The adsorption kinetic data are well fitted with the pseudo-first-order Lageren equation at the initial Cr(VI) concentration from 0.10 to 8.00 mM. Both the experimental and modeled data indicate that Friedel's salt can adsorb a large amount of Cr(VI) (up to 1.4 mmol Cr(VI)/g) very quickly (t1/2 = 2-3 min) with a very high efficiency (>99% Cr(VI) removal at [Cr] < 4.00 mM with 4.00 g/L of adsorbent) in the pH range of 4-10. In particular, the competitive adsorption tests show that the Cr(VI) removal efficiency is only slightly affected by the co-existence of Cl(-) and HCO3(-). The Cr(VI)-fixation stability tests show that only less than 0.2% adsorbed Cr(VI) is leaching out in water at pH 4-10 for 24 h because the adsorption/exchange of Cr(VI) with Friedel's salt leads to the formation of a new stable phase (3CaO x Al2O3 x CaCrO4 x 10 H2O). This research thus suggests that Friedel's salt is a potential cost-effective adsorbent for Cr(VI) removal in wastewater treatment.
Publisher: American Chemical Society (ACS)
Date: 06-01-2020
Abstract: Calcium phosphate (CP) nanoparticles (NPs) have been used in various applications for delivery of nucleic acid (NA) cargos with ideal biocompatibility and safety. However, some critical issues such as poor stability and aggregation in water solution hinder the industrial application of CP NPs. To further utilize CP NPs for NA delivery, this study specifically focused on the modification of CP NPs to achieve a rapid synthesis and improvement on dispersibility and colloidal stability by using a bisphosphonate (BP) and BSA (named as BCP NPs). Compared with CP NPs, BCP NPs show better stability and dispersity in the cell culture medium, higher efficiency in cellular uptake, and faster dissolution in acidic environments, which are essential requirements for NA vaccine delivery. The cell viability (MTT) assay indicates that BCP NPs have a similar or lower cytotoxicity than free alendronate and Lipofectamine 2000 reagent (L2K) to macrophages (MΦs), a type of typical antigen-presenting cells (APCs). Furthermore, BCP NPs exhibited 85% plasmid DNA (pDNA) loading efficiency and a good endosome escape property. Using a plasmid expressing enhanced green fluorescent protein (pEGFP) as a model system, we showed that BCP NP transfection resulted in a high-level EGFP expression in MΦs, which was even greater than the commercial L2K and electroporation method. This is the first application of a low amount of BP and BSA to modify CP-based NPs with low MΦ cytotoxicity and favorable dispersity, and our data suggest these BCP NPs are an excellent delivery platform for pDNA to MΦs.
Publisher: American Chemical Society (ACS)
Date: 29-03-2019
DOI: 10.1021/JACS.8B13898
Abstract: Biomedical investigations reveal that excessive formaldehyde generation is possibly a critical factor for tissue cancerization, cancer progression, and metastasis. Responsive molecular probes that can detect lysosomal formaldehyde in live cells and tumors and monitor drug-triggered formaldehyde scavenging contribute potentially to future cancer diagnosis and treatment monitoring. Herein, a novel "dual-key-and-lock" strategy-based ruthenium(II) complex probe, Ru-FA, is reported as an effective tool for formaldehyde detection in vitro and in vivo. Ru-FA shows weak luminescence due to photon-induced electron transfer (PET) process from Ru(II) center to electron withdrawing group 2,4-dinitrobenzene (DNB). Triggered by the specific reaction with formaldehyde (first "key") in an acidic microenvironment (second "key"), DNB is cleaved from Ru-FA, affording an emissive Ru(II) complex derivative, Ru-NR. Spectrometric analysis including steady-state and time-gated luminescence indicates that Ru-FA is favorable to be used as the probe for quantification of formaldehyde in human sera and mouse organs. Ru-FA is biocompatible and cell membrane permeable. Together with its smart "dual-key-and-lock" response to formaldehyde, luminescence imaging of lysosomal formaldehyde in live cells, visualization of tumor-derived endogenous formaldehyde, and monitoring of formaldehyde scavenging in mice were achieved, followed by the successful demonstration on detection of formaldehyde in tumors and other organs. These in vivo and in vitro detection confirm not only the excessive formaldehyde generation in tumors, but also the efficient drug administration to scavenge formaldehyde, demonstrating the potential application of Ru-FA in cancer diagnosis and treatment monitoring through lysosomal formaldehyde detection.
Publisher: American Chemical Society (ACS)
Date: 07-2010
DOI: 10.1021/JP102724S
Publisher: Wiley
Date: 13-12-2007
Publisher: Royal Society of Chemistry (RSC)
Date: 2010
DOI: 10.1039/B926239C
Publisher: Elsevier BV
Date: 03-2010
DOI: 10.1016/J.BIOMATERIALS.2009.10.058
Abstract: Although siRNAs have surpassed expectations in experiments to alter gene expression in vitro, the lack of an efficient in vivo delivery system still remains a challenge in siRNA therapeutics development and has been recognized as a major hurdle for clinical applications. In this paper we describe an inorganic nanoparticle-based delivery system that is readily adaptable for in vivo systems. Layered double hydroxide (LDH) nanoparticles, a family of inorganic crystals, tightly bind, protect, and release siRNA molecules and deliver them efficiently to mammalian cells in vitro. The uptake of siRNA-loaded LDH nanoparticles occurs via endocytosis, whereby the nanoparticles dissolve due to the low pH in the endosome, thereby aiding endosomal escape into the cytoplasm. The influence of LDH nanoparticles on cell viability and proliferation is negligible at concentrations <or=0.050 mg mL(-1), and a pronounced down-regulation of protein expression upon LDH mediated siRNA transfection of HEK293T cells is observed.
Publisher: American Chemical Society (ACS)
Date: 11-10-2007
DOI: 10.1021/ES071779G
Abstract: Zeolite matrix FAU is applied as an effective support that can be readily exchanged with ferric ions simply by wet ion exchange. The high exchange degree in this zeolite (USY) probably benefits from its larger channel dimension, which makes the diffusion of hydrated ferric ions into the channels easier. The as-prepared and subsequently calcined Fe-USY s les contain various kinds of iron species, which enable Fe-USY to efficiently decompose N2O to N2 and O2. The presence of O2 (20%), H2O (2%), or both reduces the N2O conversion only to a small extent at the same temperature. To test the feasibility and the catalytic activity in a practical situation, we prepared a monolithic Fe-USY/cordierite s le and investigated the N2O conversion under similar conditions. Such a cordierite-supported Fe-USY catalyst (approximately 9 wt % USY and 0.5 wt % Fe) shows the catalytic performance in N20 decomposition similar to the pure Fe-USY catalyst. Remarkably, both the pure Fe-USY and Fe-USY/cordierite catalysts demonstrate a very good durability because there is no activity lost after 100 and 144 h tests. Thus, the Fe-USY zeolite shows its potential as a cost-effective catalyst for N2O elimination in future applications.
Publisher: American Chemical Society (ACS)
Date: 12-02-2021
Abstract: Bone and bone-related diseases are the major cause of mobility hindrance and mortality in humans and there is no effective and safe treatment for most of them, especially, for bone and bone metastatic cancers. Bisphosphonates (BPs) are a group of small-molecule drugs for treating osteoporosis and bone cancers but have a very short half-life in circulation, requiring high doses and long-term repeat use that can cause severe side effects. Previous attempts of using nanoparticles to deliver BPs have issues of drug loading capacity and endosome escape/drug release. The present study reports the direct synthesis of BP nanoparticles by precipitating bone-favorable calcium ions and a third-generation BP, risedronate (Ca-RISNPs), to achieve high drug loading, endosomal release, and strong bone-targeting properties. The Ca-RISNPs are monodispersed with high stability at physiological pH but readily dissociate at endosomal pH conditions. They demonstrate strong penetration ability and uniform distribution in human bone and cartilage tissues and the superior drug and DNA (plasmid and oligo double strand DNA) delivery capacity in bone cells. These NPs also exhibit high specificity in killing tumor-associated macrophages (TAMs) and inhibit TAM-induced tumor cell migration. Collectively, our data indicate that this BP nanodrug platform has a great potential in managing bone-related diseases and cancers as a prolonged BP nanodrug and simultaneously as the bone-targeted drug delivery system.
Publisher: Elsevier BV
Date: 03-2010
DOI: 10.1016/J.BIORTECH.2009.07.003
Abstract: This research investigated the anaerobic biodegradation of fresh leachate from pretreated municipal solid waste (MSW) in an expanded granular sludge bed (EGSB) bioreactor under mesophilic conditions. The observations showed that this bioreactor, inoculated with anaerobic granular sludge, could be readily activated. The chemical oxygen demand (COD) removal efficiency varied between 88% and 97% under normal operation conditions, and was kept at 94-96% under the proposed optimal conditions. We noted that 60-80% of the produced biogas was methane that was yielded at a rate depending on the organic loading rate (OLR) and the liquid up-flow velocity (Vup). Significantly, 80% of loaded COD or 83% of biodegraded COD was converted to methane under the proposed optimal conditions. These findings indicate that the fresh leachate from pretreated MSW can be efficiently treated in the EGSB bioreactor, and moreover, methane, a renewable energy, can be continuously generated.
Publisher: American Chemical Society (ACS)
Date: 18-06-2004
DOI: 10.1021/CM0497529
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C7DT03725B
Abstract: OVA-CpG combined vaccines adjuvanted with both layered double hydroxide nanomaterials induced significantly stronger humoral and cell-medicated immune responses.
Publisher: Elsevier BV
Date: 05-2009
DOI: 10.1016/J.JCIS.2009.02.022
Abstract: Effective control and removal of nitrogen oxides (NO(x)) emission from vehicles exhausts under lean-burn condition is one of the most important targets in scientific research of environmental protection. A comprehensive introduction of NO(x) storage and reduction (NSR), the most promising lean-NO(x) control technology, is given including the sum-up of NSR materials, catalytic activity and related reaction mechanisms. Emphasis is put on the novel multifunctional NSR catalysts, derived from hydrotalcite-like compounds, with characteristic of simultaneous NO(x) strorage-decomposition-reduction. Finally, future research directions in the area of lean-NO(x) control based on mixed oxide catalysts derived from hydrotalcite-like materials is also proposed.
Publisher: Elsevier BV
Date: 11-2014
DOI: 10.1016/J.BIOMATERIALS.2014.07.055
Abstract: Modulation of the immune response is an important step in the induction of protective humoral and cellular immunity against pathogens. In this study, we investigated the possibility of using a nanomaterial conjugated with the toll-like receptor (TLR) ligand CpG to modulate the immune response towards the preferred polarity. MgAl-layered double hydroxide (LDH) nanomaterial has a very similar chemical composition to Alum, an FDA approved adjuvant for human vaccination. We used a model antigen, ovalbumin (OVA) to demonstrate that MgAl-LDH had comparable adjuvant activity to Alum, but much weaker inflammation. Conjugation of TLR9 ligand CpG to LDH nanoparticles significantly enhanced the antibody response and promoted a switch from Th2 toward Th1 response, demonstrated by a change in the IgG2a:IgG1 ratio. Moreover, immunization of mice with CpG-OVA-conjugated LDH before challenge with OVA-expressing B16/F10 tumor cells retarded tumor growth. Together, these data indicate that LDH nanomaterial can be used as an immune adjuvant to promote Th1 or Th2 dominant immune responses suitable for vaccination purposes.
Publisher: Elsevier BV
Date: 13-10-2010
Publisher: American Chemical Society (ACS)
Date: 22-03-2021
Publisher: Elsevier BV
Date: 02-2012
DOI: 10.1016/J.JCIS.2011.10.036
Abstract: Hydrocalumite (CaAl-LDH-Cl) was synthesized through a rehydration method involving a freshly prepared tricalcium aluminate (C(3)A) with CaCl(2) solution. To understand the intercalation behavior of sodium dodecylsulfate (SDS) with CaAl-LDH-Cl, X-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscopy (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), inductively coupled plasma-atomic emission spectrometer (ICP), and elemental analysis have been undertaken. The sorption isotherms with SDS reveal that the maximum sorption amount of SDS by CaAl-LDH-Cl could reach 3.67 mmol g(-1). The results revealed that CaAl-LDH-Cl holds a self-dissolution property, about 20-30% of which is dissolved. And the dissolved Ca(2+) and Al(3+) ions are combined with SDS to form CaAl-SDS or Ca-SDS precipitation. It has been highlighted that the composition of resulting products is strongly dependent upon the SDS concentration. With increasing SDS concentrations, the main resulting product changes from CaAl-SDS to Ca-SDS, and the value of interlayer spacing increased to 3.27 nm.
Publisher: Springer Science and Business Media LLC
Date: 22-10-2011
Publisher: Anser Press Pte. Ltd.
Date: 14-06-2023
DOI: 10.58567/CI02010003
Abstract: Cancer chemotherapy is generally associated with many severe adverse effects. Many cancer studies are currently focused on repurposing conventional non-toxic anti-parasite drugs for cancer treatment. Since cancer cells and parasites have many features in common, some anti-parasite drugs such as benzimidazoles have been recently found to possess the anti-cancer activity. Benzimidazoles act against cancer by inhibiting tubulin polymerization, inducing cancer cell apoptosis, arresting cell cycle and over-generating reactive oxygen specimen. In this review, we summarize the anticancer features of these drugs in recent investigations, lead to reconsideration of benzimidazoles as a family of anti-cancer chemotherapeutics with non-toxicity or low toxicity to the normal cells and tissues. We particularly highlight the recent progresses using nanoformulations for enhanced cancer therapy and provide our prospects in the future research.
Publisher: Elsevier BV
Date: 09-2011
DOI: 10.1016/J.SCITOTENV.2011.07.014
Abstract: Biopharmaceutical R&D complexes are major emission sources of volatile organic compounds (VOCs), which may pose potential health risks for staff on site and residents nearby. In this paper health risk assessments were performed for the VOCs in the ambient air of a typical biopharmaceutical R&D complex in China. Results showed halogenated and alkyl compounds were dominant components among 24 major VOCs from 9 selected s ling sites, inside or around the complex. The principal component analysis (PCA) indicated VOCs were generated predominantly from the biopharmaceutical research activities (factor 1 (F1), 71.6%) and traffic vehicles (factor 2 (F2), 15.4%), which were confirmed by contour maps of five selected VOCs (benzene, toluene, chlorobenzene, methylene chloride and n-hexane) simulated by Golden Software Surfer. The cumulative cancer risks for the staff on site and residents nearby were investigated and results showed the risk value were 1.01E--5 and 2.03E--5, respectively, higher than the threshold value of 1.0E--6. These results indicated that elevated VOCs from biopharmaceutical R&D complex are potential risks to the public health. Furthermore, the human health risk assessment revealed that 1,2-dichloroethane, methylene chloride, carbon tetrachloride and benzene were the dominant risk contributors for staff on site, while methyl chloride, carbon tetrachloride, 1,2-dichloroethane and tetrahydrofuran for residents nearby. As a conclusion, this work suggests that proper control strategy should be taken for VOCs releasing to minimize the public health risks, especially for the halogenated compounds.
Publisher: Springer Science and Business Media LLC
Date: 16-05-2022
DOI: 10.1038/S41477-022-01152-8
Abstract: Whitefly (Bemisia tabaci) is a phloem-feeding global agricultural pest belonging to the order Hemiptera. Foliar application of double-stranded RNA (dsRNA) represents an attractive avenue for pest control however, limited uptake and phloem availability of the dsRNA has restricted the development of RNA interference (RNAi)-based biopesticides against sap-sucking insects. Following high-throughput single and combinational target gene identification for additive effects, we report here that foliar application of dsRNA loaded onto layered double hydroxide (LDH), termed BioClay, can effectively disrupt multiple whitefly developmental stages in planta. Adjuvants were shown to enhance uptake and movement of foliar-applied dsRNA to vascular bundles and into the whitefly. Notably, delivering the dsRNA as a BioClay spray instead of as naked dsRNA improved protection against immature insect stages, demonstrating the platform's potential to extend the benefits offered by RNA insecticides towards complete life cycle control of whitefly and potentially other pests.
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7TB01635B
Abstract: New hybrid nanoparticles can efficiently deliver dsDNA/siRNA to cancer cells, with the gene release precisely controlled in the endosomal pH range.
Publisher: Wiley
Date: 14-04-2018
Abstract: Nanomaterials have been widely tested as new generation vaccine adjuvants, but few evoke efficient immunoreactions. Clay nanoparticles, for ex le, layered double hydroxide (LDH) and hectorite (HEC) nanoparticles, have shown their potent adjuvanticity in generating effective and durable immune responses. However, the mechanism by which clay nanoadjuvants stimulate the immune system is not well understood. Here, it is demonstrated that LDH and HEC-antigen complexes form loose agglomerates in culture medium/serum. They also form nodules with loose structures in tissue after subcutaneous injection, where they act as a depot for up to 35 d. More importantly, clay nanoparticles actively and continuously recruit immune cells into the depot for up to one month, and stimulate stronger immune responses than FDA-approved adjuvants, Alum and QuilA. Sustained antigen release is also observed in clay nanoparticle depots, with 50-60% antigen released after 35 d. In contrast, Alum-antigen complexes show minimal antigen release from the depot. Importantly, LDH and HEC are more effective than QuilA and Alum in promoting memory T-cell proliferation. These findings suggest that both clay nanoadjuvants can serve as active vaccine platforms for sustained and potent immune responses.
Publisher: Elsevier BV
Date: 06-2009
Publisher: American Chemical Society (ACS)
Date: 27-04-2012
DOI: 10.1021/JP300045U
Publisher: Elsevier BV
Date: 05-2011
DOI: 10.1016/J.BIORTECH.2010.11.056
Abstract: This research investigated the calcium effect on the anaerobic treatment of fresh leachate in an expanded granular sludge bed (EGSB) bioreactor under mesophilic conditions. The observations show that the bioreactor, inoculated with anaerobic granular sludge, can be started up only in about 40 days for the treatment of calcium-containing fresh leachate with chemical oxygen demand (COD) removal efficiency above 90% and organic loading rate up to 72.84 kg COD/m(3) day. The calcium accumulation onto the granules was monotonically related to the calcium concentration, accounting for 17-18 wt.% of Ca in the suspended solid in the form of calcium carbonate, phosphates hosphonates and carboxylates. The mineral formation significantly increased the granule settling velocity (by ∼ 50%) and the suspended solid concentration (by ∼ 100%). However, the effect of calcium precipitation on the specific methanogenic activity and the CH(4) production rate was complex, first positive during the start-up but later on negative.
Publisher: Elsevier BV
Date: 2013
Publisher: Elsevier BV
Date: 08-2014
DOI: 10.1016/J.JHAZMAT.2014.07.001
Abstract: The wide use of metal electroplating involving CN(-) necessitates the cost-effective treatment of both CN and metals (Zn, Cu, Ni etc.). In this research, we developed a novel strategy - Ni(2+)-assisted layered double hydroxide (LDH) precipitation - to simultaneously remove aqueous CN and Zn/Ni metals. The strategy is to convert CN(-)/Zn(CN)4(2-) to Ni(CN)4(2-) first, and then to quickly precipitate Ni(CN)4(2-)/CN(-) into LDH crystals. The conversion has been clearly evidenced by the change of CN characteristic FTIR bands of Zn-CN solution before and after adding Ni(NO3)2. The intercalation and efficient removal of CN have also been confirmed through the formation of LDH crystals XRD and SEM. In particular, a set of optimized experimental factors has been obtained by investigating their effects on CN removal efficiency in the simulated tests. Remarkably, over 95% CN were removed with high removal efficiencies of metals. Our results thus suggest that the current strategy is a quick, efficient and promising way to simultaneously treat both Ni and metals/CN rich electroplating wastewaters.
Publisher: Elsevier BV
Date: 02-2019
DOI: 10.1016/J.COLSURFB.2018.11.035
Abstract: Antibiotic-resistant bacterial infections are a global health problem. A commonly-used antibiotic Penicillin G was incorporated into ZnAl-layered double hydroxides (PNG/LDH) with a varied amount of PNG. PNG/LDH nanocomposites were well characterized in structure and composition using elemental analysis, X-ray diffraction pattern, Fourier transform infrared spectroscopy and TEM images, revealing that PNG were mostly adsorbed on the LDH surfaces at a lower PNG loading but some were intercalated into LDH interlayers at a higher PNG loading. The typical release profile of PNG and Zn
Publisher: Elsevier BV
Date: 10-2013
Publisher: American Chemical Society (ACS)
Date: 07-12-2008
DOI: 10.1021/ES072085A
Abstract: Various XnY(3-n)Al-hydrotalcite-like compounds (HTlcs) were synthesized by the constant pH coprecipitation method with the (X2+ + Y2+)/Al3+ molar ratio fixed at 3.0. Well-mixed oxides Xn,Y(3-n)AlO were derived from corresponding HTlcs precursors upon calcination. Physicochemical characterization with X-ray diffraction analysis, Fourier transform infrared spectroscopy, thermogravimetric analysis (TGA), and the Brunauer-Emmett-Teller equation indicates that the derived oxides are of either periclase or spinel phase, with an interparticle pore diameter of 9.6-15.4 nm. These oxides generally show a high CO2 adsorption capability at 350 degrees C. For ex le, CaCoAlO captures 1.39 mmol/g of CO2 (i.e., 6.12 wt %) from a gas mixture (8% CO2 in N2) at 350 degrees C and 1 atm in a fixed-bed reactor within 20 min. All other mixed oxides adsorb 0.87-1.28 mmol/g (3.83-5.63 wt %) of CO2. Therefore, these mixed oxides are potential cost-effective CO2 sorbents for environmental remediation. In addition, the CO2 adsorption behavior is well-described with the deactivation model. The species of CO2 formed on the sorbents are various carbonates, as revealed by in situ IR spectra as well as TGA.
Publisher: American Chemical Society (ACS)
Date: 02-02-2009
DOI: 10.1021/JP810112C
Publisher: American Chemical Society (ACS)
Date: 12-10-2001
DOI: 10.1021/CM010347G
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D2BM01432G
Abstract: A biomimetic CpG-layered double hydroxide nanoparticle-engineered cancer cell membrane nano-vaccine with mannose conjugation to boost anti-cancer immunity with a long-term anti-tumor immune response was proposed.
Publisher: Elsevier BV
Date: 09-2019
DOI: 10.1016/J.COLSURFB.2019.06.013
Abstract: The search for effective alternatives to traditional antibiotics to avoid antibiotic resistant bacteria is growing worldwide. ZnO nanoparticles are found to effectively inhibit growth and proliferation of bacteria, and ZnO-based layered double hydroxides (ZnO-based LDHs) have been intensively investigated for this purpose. However, the nanocomposites are made in a multi-step preparation process with severe agglomeration and limited bactericidal ability. In this research, ZnO-dotted nanohybrids using Zn
Publisher: Royal Society of Chemistry (RSC)
Date: 2009
DOI: 10.1039/B819379G
Publisher: Elsevier BV
Date: 05-2022
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1TB01408K
Abstract: The ATP-modified Calcium Phosphate (ACP) nano-adjuvant for DNA vaccines. ATP plays two key roles in this system: (1) a stabiliser for CP to inhibit the particle size growth and aggregation, and (2) the immunological adjuvant for DNA vaccine.
Publisher: American Chemical Society (ACS)
Date: 26-09-2017
Publisher: Springer Science and Business Media LLC
Date: 30-10-2021
DOI: 10.1186/S12951-021-01096-9
Abstract: Multi-modal therapy has attracted increasing attention as it provides enhanced effectiveness and potential stimulation of the immune community. However, low accumulation at the tumor sites and quick immune clearance of the anti-tumor agents are still insurmountable challenges. Hypothetically, cancer cell membrane (CCM) can homologously target the tumor whereas multi-modal therapy can complement the disadvantages of singular therapies. Meanwhile, moderate hyperthermia induced by photothermal therapy can boost the cellular uptake of therapeutic agents by cancer cells. CCM-cloaked indocyanine green (ICG)-incorporated and abraxane (PTX-BSA)-loaded layered double hydroxide (LDH) nanosheets (LIPC NSs) were fabricated for target efficient photo-chemotherapy of colorectal carcinoma (CRC). The CCM-cloaked LDH delivery system showed efficient homologous targeting and cytotoxicity, which was further enhanced under laser irradiation to synergize CRC apoptosis. On the other hand, CCM-cloaking remarkably reduced the uptake of LDH NSs by HEK 293T cells and macrophages, implying mitigation of the side effects and the immune clearance, respectively. In vivo data further exhibited that LIPC NSs enhanced the drug accumulation in tumor tissues and significantly retarded tumor progression under laser irradiation at very low therapeutic doses (1.2 and 0.6 mg/kg of ICG and PTX-BSA), without observed side effects on other organs. This research has demonstrated that targeting delivery efficiency and immune-escaping ability of LIPC NSs are tremendously enhanced by CCM cloaking for efficient tumor accumulation and in situ generated hyperthermia boosts the uptake of LIPC NSs by cancer cells, a potential effective way to improve the multi-modal cancer therapy.
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/C9TB02515D
Abstract: Heparinization of polymeric filtration membranes enhances antithrombogenicity and anti-fouling while maintaining the permeable property, increasing the membrane life time and reducing the patients’ economic burden.
Publisher: American Chemical Society (ACS)
Date: 17-02-2007
DOI: 10.1021/JP0683723
Publisher: Elsevier BV
Date: 04-2007
DOI: 10.1016/J.JCIS.2006.12.052
Abstract: We report the preparation and characterization of a novel nanocomposite adsorbent for anionic dye removal. The nanocomposite adsorbent was prepared by heterocoagulation of delaminated bentonite and layered double hydroxide (LDH) colloids. The effects of preparation conditions, LDH loading, particle size, and calcination temperature of the modified material on the physicochemical properties of this composite adsorbent have been investigated. The optimal conditions for best Reactive Yellow 2 (RY2) dye removal efficiency are a weight ratio of LDH to bentonite of 1:1, LDH particle size 100 nm, and calcination temperature 673 K. The adsorption equilibrium data can be fitted well by the widely accepted adsorption isotherm models.
Publisher: Wiley
Date: 19-11-2015
Abstract: Colon cancer is one of the most common cancers worldwide with high mortality. A major issue in colon cancer treatment is drug-resistance and metastasis that have been ascribed to the cancer stem cells. In this study, colon cancer stem cells were isolated through sphere culture and verified with the cancer stem cell markers CD133, CD44, and CD24. It was demonstrated that the PI3K/Akt/mTOR signalling pathway was highly activated in the colon cancer stem cells and that inhibition of the PI3K/Akt/mTOR pathway by the inhibitor BEZ235 suppressed the colon cancer stem cell proliferation with reduced stemness indicated by CD133 and Lgr5 expressions. Treatment with insulin as a known activator of the PI3K/Akt pathway increased CD133 expression and decreased the effects of BEZ235 on colon cancer proliferation and survival. The data presented here collectively suggest that the PI3K/Akt/mTOR pathway underpins the stemness of colon cancer stem cells and BEZ235 is potentially a good drug candidate for treatment of colon cancer drug resistance and metastasis.
Publisher: Elsevier BV
Date: 2015
DOI: 10.1016/J.JCIS.2014.09.010
Abstract: It is well known that delivery efficiency to cells is highly dependent on particle size and the administered dose. However, there is a marked discrepancy in many reports, mainly due to the inconsistency in assessment of various parameters. In this particular research, we designed experiments using layered double hydroxide nanoparticles (LDH NPs) to specifically elucidate the effect of particle size, dose and dye loading manner on cellular uptake. Using the number of LDH NPs taken up by HCT-116 cells as the indicator of delivery efficiency, we found that (1) the size of sheet-like LDH in the range of 40-100 nm did not significantly affect their cellular uptake (2) cellular uptake of 40 and 100 nm LDH NPs was increased proportionally to the number concentration below a critical value, but remained relatively constant beyond the critical value and (3) the effect of the dye loading manner is mainly dependent on the loading capacity or yield. In particular, the loading capacity is determined by the NP specific surface area. This research may be extended to a larger size range to examine the size effect, but suggests that it is necessary to set up a protocol to evaluate the effects of NP's physicochemical properties on the cellular delivery efficiency.
Publisher: Wiley
Date: 15-12-2014
Abstract: Although many studies reporting the organ-level biodistribution of nanoparticles (NPs) in animals, very few have addressed the fate of NPs in organs at the cellular level. The liver appears to be the main organ for accumulation of NPs after intravenous injection. In this study, for the first time, the in vivo spatiotemporal disposition of recently developed mercaptosuccinic acid (MSA)-capped cadmium telluride/cadmium sulfide (CdTe/CdS) quantum dots (QDs) is explored in rat liver using multiphoton microscopy (MPM) coupled with fluorescence lifetime imaging (FLIM), with subcellular resolution (∼1 μm). With high fluorescence efficiency and largely improved stability in the biological environment, these QDs show a distinct distribution pattern in the liver compared to organic dyes, rhodamine 123 and fluorescein. After intravenous injection, fluorescent molecules are taken up by hepatocytes and excreted into the bile, while negatively charged QDs are retained in the sinusoids and selectively taken up by sinusoidal cells (Kupffer cells and liver sinusoidal endothelial cells), but not by hepatocytes within 3 h. The results could help design NPs targeting the specific types of liver cells and choose the fluorescent markers for appropriate cellular imaging.
Publisher: American Chemical Society (ACS)
Date: 27-01-2021
Publisher: Elsevier BV
Date: 08-2008
DOI: 10.1016/J.JCONREL.2008.05.021
Abstract: Current investigations show that layered double hydroxide (LDH) nanoparticles have high potential as effective non-viral agents for cellular drug delivery due to their low cytotoxicity, good biocompatibility, high drug loading, control of particle size and shape, targeted delivery and drug release control. Two types of Mg(2)Al-LDH nanoparticles with fluorescein isothiocyanate (FITC) were controllably prepared. One is morphologically featured as typical hexagonal sheets (50-150 nm laterally wide and 10-20 nm thick), while the other as typical rods (30-60 nm wide and 100-200 nm long). These LDH(FTIC) nanoparticles are observed to immediately transfect into different mammalian cell lines. We found that internalized LDH(FITC) nanorods are quickly translocated into the nucleus while internalized LDH(FITC) nanosheets are retained in the cytoplasm. Inhibition experiments show that the cellular uptake is a clathrin-mediated time- and concentration-dependent endocytosis. Endosomal escape of LDH(FITC) nanoparticles is suggested to occur through the deacidification of LDH nanoparticles. Since quick nuclear targeting of LDH(FITC) nanorods requires an active process, and although the exact mechanism is yet to be fully understood, it probably involves an active transport via microtubule-mediated trafficking processes. Targeted addressing of two major subcellular compartments by simply controlling the particle morphology/size could find a number of applications in cellular biomedicine.
Publisher: Elsevier BV
Date: 07-2008
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0NR04881J
Abstract: We report a photochromic molecule doped polymer nanoprobe in which the emission can be modulated by light and lysosomal hydrogen peroxide for multicolour fluorescence “double-check” imaging.
Publisher: CRC Press
Date: 23-03-2004
Publisher: Elsevier BV
Date: 03-2014
DOI: 10.1016/J.BIOMATERIALS.2013.12.095
Abstract: In this research we employed layered double hydroxide nanoparticles (LDHs) to simultaneously deliver an anticancer drug 5-fluorouracil (5-FU) and Allstars Cell Death siRNA (CD-siRNA) for effective cancer treatment. The strategy takes advantage of the LDH anion exchange capacity to intercalate 5-FU into its interlayer spacing and load siRNA on the surface of LDH nanoparticles. LDH nanoparticles have been previously demonstrated as an effective cellular delivery system for 5-FU and siRNA separately in various investigations. More excitedly, the combination of CD-siRNA and anticancer drug 5-FU with the same LDH particles significantly enhanced cytotoxicity to three cancer cell lines, e.g. MCF-7, U2OS and HCT-116, compared to the single treatment with either CD-siRNA or 5-FU. This enhancement is probably a result of coordinate mitochondrial damage process. Thus, the strategy to co-deliver siRNA and an anticancer drug by LDHs has great potential to overcome the drug resistance and enhance cancer treatment.
Publisher: American Chemical Society (ACS)
Date: 17-11-2001
DOI: 10.1021/NL010045D
Publisher: American Chemical Society (ACS)
Date: 08-2006
DOI: 10.1021/JP062281O
Abstract: We report a simple but efficient method to prepare stable homogeneous suspensions containing monodispersed MgAl layered double hydroxide (LDH) nanoparticles that have wide promising applications in cellular drug (gene) delivery, polymer/LDH nanocomposites, and LDH thin films for catalysis, gas separation, sensing, and electrochemical materials. This new method involves a fast coprecipitation followed by controlled hydrothermal treatment under different conditions and produces stable homogeneous LDH suspensions under variable hydrothermal treatment conditions. Moreover, the relationship between the LDH particle size and the hydrothermal treatment conditions (time, temperature, and concentration) has been systematically investigated, which indicates that the LDH particle size can be precisely controlled between 40 and 300 nm by adjusting these conditions. The reproducibility of making the identical suspensions under identical conditions has been confirmed with a number of experiments. The dispersion of agglomerated LDH aggregates into in idual LDH crystallites during the hydrothermal treatment has been further discussed. This method has also been successfully applied to preparing stable homogeneous LDH suspensions containing various other metal ions such as Ni(2+), Fe(2+), Fe(3+), Co(2+), Cd(2+), and Gd(3+) in the hydroxide layers and many inorganic anions such as Cl(-), CO(3)(2-), NO(3)(-), and SO(4)(2-).
Publisher: American Chemical Society (ACS)
Date: 07-08-2018
Publisher: Royal Society of Chemistry (RSC)
Date: 04-12-2003
DOI: 10.1039/B207540G
Publisher: American Chemical Society (ACS)
Date: 31-01-2012
DOI: 10.1021/CM2033417
Publisher: MDPI AG
Date: 26-11-2014
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D3GC02995F
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C3TB20202J
Publisher: Wiley
Date: 22-03-2005
Publisher: American Chemical Society (ACS)
Date: 20-12-2014
DOI: 10.1021/ES403884E
Abstract: In this investigation, kirschsteinite-dominant stainless steel slag (SSS) has been found to decompose sulfur hexafluoride (SF6) with the activity higher than pure metal oxides, such as Fe2O3 and CaO. SSS is mainly made up of CaO·FeO·SiO2(CFS)/MgO·FeO·MnO(RO) phase conglomeration. The SF6 decomposition reaction with SSS at 500-700 °C generated solid MF2/MF3 and gaseous SiF4, SO2/SO3 as well as HF. When 10 wt % of SSS was replaced by Fe2O3 or CaO, the SF6 decomposition amount decreased from 21.0 to 15.2 or 15.0 mg/g at 600 °C. The advantage of SSS over Fe2O3 or CaO in the SF6 decomposition is related to its own special microstructure and composition. The dispersion of each oxide component in SSS reduces the sintering of freshly formed MF2/MF3, which is severe in the case of pure metal oxides and inhibits the continuous reaction of inner components. Moreover, SiO2 in SSS reacts with SF6 and evolves as gaseous SiF4, which leaves SSS with voids and consequently exposes inner oxides for further reactions. In addition, we have found that oxygen significantly inhibited the SF6 decomposition with SSS while H2O did not, which could be explained in terms of reaction pathways. This research thus demonstrates that waste material SSS could be potentially an effective removal reagent of greenhouse gas SF6.
Publisher: American Chemical Society (ACS)
Date: 26-06-2007
DOI: 10.1021/JP0678352
Publisher: MDPI AG
Date: 29-04-2014
DOI: 10.3390/IJMS15057409
Publisher: Elsevier BV
Date: 12-2012
Publisher: Elsevier BV
Date: 12-2013
Publisher: American Chemical Society (ACS)
Date: 28-08-2008
DOI: 10.1021/IE8004226
Publisher: Elsevier BV
Date: 07-06-2010
Publisher: Wiley
Date: 06-06-2017
Abstract: Recently, Mn(II)-containing nanoparticles have been explored widely as an attractive alternative to Gd(III)-based T
Publisher: Wiley
Date: 12-04-2007
Publisher: Springer Science and Business Media LLC
Date: 03-190728634
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4CP00514G
Abstract: Atomistic simulations elucidate the mechanism of high selectivity and low flow resistance of molecular capture in carbon nanotube networks.
Publisher: Elsevier BV
Date: 2020
DOI: 10.1016/J.JHAZMAT.2019.120935
Abstract: Manganese slag is a hazardous waste, which lacks proper treatment. For the first time, an effective catalyst for selective catalytic reduction of nitric oxide was synthesized from manganese slag by a sol-gel method. The obtained catalyst had an excellent low-temperature activity and high hydrothermal stability. It removed 46.3% of nitric oxide (990 ppm) at a temperature as low as 100 °C its removal increased to 100% at 220 °C, which lasted for more than 2000 min. Moreover, hydrothermal treatment at 400 °C showed little influence on its activity. Even after hydrothermal treatment at 900 °C, the catalyst still removed 39.7% of NO at 220 °C, 22.7% higher than another catalyst synthesized from pure reagents. The hydrothermal stability was attributed to an amorphous layer of MnSi
Publisher: Wiley
Date: 12-07-2012
Abstract: Targeted local delivery of a nanoparticle-based, antibody-targeted, and low molecular weight heparin (LMWH) delivery system successfully reduces restenosis and thrombus formation in an animal model. An antibody recognizing cross-linked fibrin (XLF) D-dimer is successfully conjugated to layered double hydroxide nanoparticles. Use of the anti-XLF-conjugated LMWH-carrying layered double hydroxide nanoparticles shows successful targeting of the nanoparticles (red) to the injured artery wall (green), resulting in decreased neointimal thickening and thrombus formation.
Publisher: Elsevier BV
Date: 04-2015
Publisher: American Chemical Society (ACS)
Date: 11-11-2019
Abstract: Overproduction of reactive oxygen species (ROS) is commonly known as a key factor in the progression of many chronic inflammation diseases such as atherosclerosis and rheumatoid arthritis. In this study, a metal oxide nanodot-coated layered double hydroxide (LDH) nanocomposite is constructed for theranostics of ROS-related diseases. This is the first time that both cerium oxide and iron oxide nanoparticles (NPs) were attached on the surface of LDH NPs through electrostatic interaction via a nanoengineering approach. LDHs served as nanocarriers, cerium oxide NPs served as therapeutic agents due to the antioxidant properties, and iron oxide NPs served as magnetic resonance imaging (MRI) contrast agents. In vitro studies have demonstrated that the constructed nanocomposites have good biocompatibility, good antioxidant capacity to reduce ROS level in the cells, as well as satisfying cell imaging effect in MRI. Functionalization of LDH surface with cerium oxide NPs and iron oxide NPs allows the simultaneous therapy and diagnosis of ROS-related diseases and may also allow biodistribution tracking of the therapeutic cerium oxide NPs.
Publisher: Wiley
Date: 03-2015
DOI: 10.1002/RMV.1825
Abstract: Cervical cancer is a major disease with high mortality. All cervical cancers are caused by infection with human papillomaviruses (HPV). Although preventive vaccines for cervical cancer are successful, treatment of cervical cancer is far less satisfactory because of multidrug resistance and side effects. In this review, we summarize the recent application of nanotechnology to the diagnosis and treatment of cervical cancer as well as the development of HPV vaccines. Early detection of cervical cancer enables tumours to be efficiently removed by surgical procedures, leading to increased survival rate. The current method of detecting cervical cancer by Pap smear can only achieve 50% sensitivity, whereas nanotechnology has been used to detect HPVs with greatly improved sensitivity. In cervical cancer treatment, nanotechnology has been used for the delivery of anticancer drugs to increase treatment efficacy and decrease side effects. Nanodelivery of HPV preventive and therapeutic vaccines has also been investigated to increase vaccine efficacy. Overall, these developments suggest that nanoparticle-based vaccine may become the most effective way to prevent and treat cervical cancer, assisted or combined with some other nanotechnology-based therapy.
Publisher: Elsevier BV
Date: 05-2013
Publisher: Elsevier BV
Date: 03-2015
Publisher: Elsevier BV
Date: 10-2017
DOI: 10.1016/J.COVIRO.2017.07.009
Abstract: Exogenous application of double-stranded RNA (dsRNA) for virus resistance in plants represents a very attractive alternative to virus resistant transgenic crops or pesticides targeting virus vectors. However, the instability of dsRNA sprayed onto plants is a major challenge as spraying naked dsRNA onto plants provides protection against homologous viruses for only 5 days. Innovative approaches, such as the use of nanoparticles as carriers of dsRNA for improved stability and sustained release, are emerging as key disruptive technologies. Knowledge is still limited about the mechanism of entry, transport and processing of exogenously applied dsRNA in plants. Cost of dsRNA and regulatory framework will be key influencers towards practical adoption of this technology.
Publisher: Oxford University Press (OUP)
Date: 09-07-2021
Abstract: Topical application of double-stranded RNA (dsRNA) can induce RNA interference (RNAi) and modify traits in plants without genetic modification. However, delivering dsRNA into plant cells remains challenging. Using developing tomato (Solanum lycopersicum) pollen as a model plant cell system, we demonstrate that layered double hydroxide (LDH) nanoparticles up to 50 nm in diameter are readily internalized, particularly by early bicellular pollen, in both energy-dependent and energy-independent manners and without physical or chemical aids. More importantly, these LDH nanoparticles efficiently deliver dsRNA into tomato pollen within 2–4 h of incubation, resulting in an 89% decrease in transgene reporter mRNA levels in early bicellular pollen 3-d post-treatment, compared with a 37% decrease induced by the same dose of naked dsRNA. The target gene silencing is dependent on the LDH particle size, the dsRNA dose, the LDH–dsRNA complexing ratio, and the treatment time. Our findings indicate that LDH nanoparticles are an effective nonviral vector for the effective delivery of dsRNA and other biomolecules into plant cells.
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7CP02701J
Abstract: Membrane interactions are critical for the successful use of inorganic nanoparticles as antimicrobial agents and as carriers of, or co-actives with, antimicrobial peptides (AMPs).
Publisher: Elsevier BV
Date: 04-2008
Publisher: Elsevier BV
Date: 10-2008
DOI: 10.1016/J.JCIS.2008.06.062
Abstract: In this research, we investigated the effect of dynamic anion adsorption/exchange on the surface charging property of Mg(2)AlClLDH and Mg(2)AlCO(3)LDH particles that show the average zeta potential of 41 and 34 mV in the as-prepared suspension, respectively. The addition of NaCl up to 3x10(-3) M in the suspension does not obviously affect the zeta potential of both LDHs, which can be attributed to the less affinity of Cl(-) to LDH. The introduction of Na(2)CO(3) severely reduces the zeta potential at the CO(3)(2-) concentration higher than 1x10(-4) M, and to the negative value in both LDH systems at ca. 2x10(-3) M, which is presumably resulted from the exchange and the re-orientation of CO(3)(2-) in a tilt/vertical style on the surface. All four organic anions (dodecyl sulfate, folate, citrate and polyacrylate) also significantly affect the zeta potential of the LDH particles. At the lower concentrations of organic anionic groups (<1x10(-4) M), the zeta potential was slightly affected, i.e. limited exchange/adsorption. However, the concentration increasing to some point suddenly decreases and reverses the zeta potential of the LDH particles, which is presumably caused by the hydrophobic interactions that bind the hydrophobic hydrocarbon chains (especially in dodecyl sulfate) into the micelle-like bilayer bunches on the LDH surface. In addition, the effect of pH in 5.5-11.0 on the LDH particle surface charging is mainly reflected through the conversion of CO(3)(2-) to HCO(3)(-)/H(2)CO(3) when pH decreases from ca. 11 to 6, with limited contribution from protonation/deprotonation and exchange/adsorption.
Publisher: American Chemical Society (ACS)
Date: 05-02-2021
Publisher: Elsevier BV
Date: 12-2013
DOI: 10.1016/J.JCIS.2013.08.008
Abstract: In this report, a detailed FTIR fitting analysis was used to recognize Mg, Zn and Al homogeneous distribution in Mg(x)Zn(y)Al(x+y)/2-Layered double hydroxide (LDH) hydroxyl layer. In detail, OH-Mg2Al:OH-Mg3 ratios decreased from 95.2:4.8 (MIR) and 94.2:5.8 (NIR) to 58.9:41.1 (MIR) and 61.8:38.2 (NIR), when Mg:Al increased from 2.2:1.0 to 4.1:1.0 in MgAl-LDHs. These fitting results were similar with theoretical calculations of 94.3:5.7 and 59.0:41.0. In a further analysis of Mg(x)Zn(y)Al(x+y)/2-LDHs, OH bonded Zn2Mg, Zn2Al, MgZnAl, Mg2Al and Mg2Zn peaks were identified at 3420, 3430, 3445-3450, 3454 and 3545 cm(-1), respectively. With the decrease of Mg:Zn from 3:1 to 1:3, metal-hydroxyl bands changed from OH-Mg2Al and MgZnAl (with a ratio of 49.4:50.6) to OH-MgZnAl and Zn2Al (with a ratio of 55.0:45.0). They were also similar with theoretical calculations of 47.6:52.4 and 54.6:45.4. As a result, these results show that there is an ordered cation distribution in Mg(x)Zn(y)Al(x+y)/2-LDH, and FTIR is feasible in recognizing this structure.
Publisher: Wiley
Date: 20-08-2019
Publisher: American Chemical Society (ACS)
Date: 02-05-2018
Publisher: American Chemical Society (ACS)
Date: 12-02-2010
DOI: 10.1021/IE901469C
Publisher: American Chemical Society (ACS)
Date: 13-01-2007
DOI: 10.1021/ES061538T
Abstract: Co(2.5)Mg(0.5)/Al1 and Co(2.5)Mg(0.5)/X(0.5)Al(0.5) hydrotalcite-like compounds (where X = Fe, Mn, Zr, La) were synthesized by a constant-pH coprecipitation. The derived oxides from hydrotalcites upon calcination at 800 degrees C for 4 h in static air are mainly of spinel phase, with a surface area of 14.2-23.8 m2/g, where new phase ZrO2 and La2O3 are segregated in Zr- and La-containing oxides, respectively. Incorporation of the fourth element has assisted the reduction of transition-metal cations in the oxide catalysts, which may lead to the enhancement of the NO storage capacity in O2 at 100 degrees C for all catalysts. However, at 300 degrees C, only Zr- and La-containing catalysts improve the NO storage performance. Substantially, La-containing catalyst excels over all other catalysts in NO storage capability both at 100 and 300 degrees C. More remarkably, the NO storage at 300 degrees C (7.56 mg/g) is much higher than that at 100 degrees C (4.69 mg/g). NO adsorption/desorption routes have been proposed to explain the NO storage, the NO-to-NO2 conversion, and the reduction (decomposition) of NO to N2O/N2 in O2 on the catalysts. In addition, the negative influences of CO2 or H2O on the NO storage/reduction have been further revealed in this research.
Publisher: Springer Science and Business Media LLC
Date: 08-01-2010
Publisher: Elsevier BV
Date: 11-2012
DOI: 10.1016/J.JHAZMAT.2012.08.039
Abstract: This paper describes the enhanced Cr(VI)-contaminated soil remediation via a combination of electrokinetics (EK) with a calcined-hydrotalcite-based permeable reactive barrier (PRB). First, this combination proved to be feasible, and remarkably facilitated Cr(VI) remediation in a column test. Then, lightly-to-severely (0.16-1.65 mg/g) Cr(VI)-contaminated soil was remediated in a simulated test with the calcined hydrotalcite as the PRB under an voltage of 10-30 V (i.e. an electric field intensity of 0.7-2.0 V/cm). The observations demonstrated that both PRB and EK are critical to efficient remediation and the high de-contamination efficiency is supposedly attributed to the synergistic effect, for which EK concentrates anionic chromate to the anode region and PRB media (calcined hydrotalcite) absorbs and immobilizes it. Thus we have shown that the combined PRB-EK system is highly adaptive and effective in remediation of a larger area contaminated with chromate and various anionic pollutants.
Publisher: MDPI AG
Date: 07-06-2012
Publisher: Wiley
Date: 22-06-2021
DOI: 10.1002/VIW.20200139
Abstract: Oxygen‐derived free radicals (ODFRs) are partially reduced oxygen reactive intermediates in living organisms during various biological processes, such as mitochondrial respirations, enzyme‐catalyzed oxidations, and radiolysis. Of various ODFRs being identified, superoxide anion (O 2 •– ) and hydroxyl (•OH) radicals are extensively investigated. Due to their high reactivity, these radicals can oxidize many biomolecules, including nucleic acids, proteins, lipids, and carbohydrates, which would damage some key cellular components. Under oxidative stress, elevated oxygen radical levels are closely associated with various diseases, such as neurodegenerative diseases, cardiovascular diseases, rheumatoid arthritis, and cancers. In this context, a number of responsive nanoprobes have been developed for luminescent detection of ODFRs in living organisms. In this review, we briefly introduce the production of ODFRs and their biological importance for various diseases. We then extensively summarize responsive nanoprobes for detection and bioimaging of these two oxygen radicals, O 2 •– and •OH, which are designed and developed based on their specific oxidation mechanisms in the recent decade. Challenges and some future research directions are also proposed as the conclusion.
Publisher: Elsevier BV
Date: 12-2006
Publisher: Bentham Science Publishers Ltd.
Date: 09-2013
Publisher: American Chemical Society (ACS)
Date: 18-12-2009
DOI: 10.1021/JP807411X
Publisher: Elsevier BV
Date: 12-2008
Publisher: Elsevier BV
Date: 08-2012
Publisher: MDPI AG
Date: 24-10-2022
Abstract: Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an infectious disease that has become a serious burden on global public health. This study screened and yielded specific nanobodies (Nbs) against SARS-CoV-2 spike protein receptor binding domain (RBD), following testing its basic characteristics. A nanobody phage library was established by immunizing a camel with RBD protein. After three rounds of panning, the positive colonies were screened by enzyme-linked immunosorbent assay (ELISA). By sequencing, four different sequences of nanobody gene fragments were selected. The four nanobody fusion proteins were expressed and purified, respectively. The specificity and affinity of the four nanobodies were identified by ELISA. Our results showed that an immune phage display library against SARS-CoV-2 has been successfully constructed with a library capacity of which was 4.7 × 108 CFU. The four purified nanobodies showed specific high-affinity binding SARS-CoV-2 S-RBD. Among these, the antigen binding affinity of Nb61 was more comparable to that of commercial rabbit anti-SARS-CoV-2 S-RBD antibodies. In sum, our study has obtained four nanobody strains against SARS-CoV-2 S-RBD with significant affinity and specificity, therefore laying an essential foundation for further research as well as the applications of diagnostic and therapeutic tools of SARS-CoV-2.
Publisher: American Chemical Society (ACS)
Date: 02-11-2018
DOI: 10.1021/ACSSENSORS.8B00776
Abstract: An effective bioanalytical method for rapid, sensitive, specific, and in situ sensing of nitric oxide (NO) is the key for further unveiling the biological functions of this gasotransmitter molecule in vitro and in vivo. In this contribution, a new fluorescence probe for sensing and imaging of NO in live systems was developed. The probe, FP-NO, was designed by exploring a novel sensing mechanism, i.e., the rotation of the N-N single bond of a coumarin derivative. FP-NO was prepared by incorporating a recognition unit, thiosemicarbazide moiety into a coumarin fluorophore. The weakly fluorescent FP-NO quickly and selectively reacts with NO to form a highly fluorescent product, FP-P. Such an enhancement of fluorescence emission allows NO detection with high sensitivity. The detection limit was 47.6 nM. The reaction mechanism was validated by HRMS titration analysis and the "OFF-ON" fluorescence response mechanism was rationalized by theoretical computation. FP-NO is biocompatible and live cell membrane permeable. The feasibility of FP-NO as the fluorescence probe for imaging and flow cytometry analysis of exogenous NO in MCF-7 cells and exogenous NO production in inflamed J774A.1 macrophage cells was then evaluated. Visualization of exogenous and endogenous NO production in live zebrafish was then achieved, implying the potential application of FP-NO in the studies of the NO roles in live organisms.
Publisher: IEEE
Date: 2006
Publisher: Elsevier BV
Date: 03-2012
DOI: 10.1016/J.JCIS.2011.12.046
Abstract: In this paper, we report the novel finding that the cellular delivery efficiency of siRNAs or their mimic double-stranded (ds)DNA using layered double hydroxide (LDH) nanoparticles is dependent upon the nucleotide sequence. Efficacy of LDH-mediated delivery of four different siRNAs into cortical neurons and NIH 3T3 cells was found to vary widely (from 6 to 80%, and 2-11%, respectively). Our investigation into the formation of dsDNA-LDH complexes through monitoring the dsDNA:LDH mass ratio at the point of zero charge (PZC) indicated that the degree of intercalation of the in idual dsDNA sequences into the LDH nanoparticles varied significantly. The dsDNA:LDH mass ratio at the PZC was found to be dependent on the nucleotide sequence. We further observed that PZC for each sequence was positively related to the extent of LDH-mediated internalization of the equivalent siRNA into neurons and fibroblasts. This novel finding therefore suggests that the mass ratio at the PZC is a useful predictive tool with which to assess the intercalation efficiency of selected siRNA sequences into the LDH interlayer and subsequent internalization into the cell cytoplasm. This finding will allow a more controlled approach to the design of suitable siRNA sequences for LDH-mediated siRNA delivery.
Publisher: Wiley
Date: 23-10-2007
Publisher: American Chemical Society (ACS)
Date: 26-07-2022
Abstract: Cancer immunotherapy efficacy is largely limited by the suppressive tumor immune microenvironment (TIME) where antitumor immune cells are inhibited and tumor antigens continue to mutate or be lost. To remodel the TIME, we here applied weakly alkaline layered double hydroxide nanoparticles (LDH NPs) to neutralize the excess acid and block autophagy of tumor cells for neoadjuvant cancer immunotherapy. Peritumoral injection of LDH NPs provided a long-term and efficient acid-neutralization in the TIME, blocked the lysosome-mediated autophagy pathway in tumor cells, and increased the levels of antitumor tumor-associated macrophages and T cells. These LDH NPs captured tumor antigens released in the tumor tissues and effectively inhibited the growth of both melanoma and colon tumors
Publisher: MDPI AG
Date: 18-07-2022
DOI: 10.3390/PHARMACEUTICS14071488
Abstract: To overcome the severe side effects of cancer chemotherapy, it is vital to develop targeting chemotherapeutic delivery systems with the potent inhibition of tumour growth, angiogenesis, invasion and migration at low drug dosages. For this purpose, we co-loaded a conventional antiworm drug, albendazole (ABZ), and a TOPK inhibitor, OTS964, into lipid-coated calcium phosphate (LCP) nanoparticles for skin cancer treatment. OTS- and ABZ-loaded LCP (OTS-ABZ-LCP) showed a synergistic cytotoxicity against skin cancer cells through their specific cancerous pathways, without obvious toxicity to healthy cell lines. Moreover, dual-targeting the programmed death ligand-1 (PD-L1) and folate receptor overexpressed on the surface of skin cancer cells completely suppressed the skin tumour growth at low doses of ABZ and OTS. In summary, ABZ and OTS co-loaded dual-targeting LCP NPs represent a promising platform with high potentials against complicated cancers where PD-L1/FA dual targeting appears as an effective approach for efficient and selective cancer therapy.
Publisher: MDPI AG
Date: 28-01-2019
DOI: 10.3390/NANO9020159
Abstract: Suitable carriers are crucial to RNAi applications for cancer genotherapy and T-cell immunotherapy. In this research, we selected two extensively-investigated biocompatible inorganic nanoparticle carriers, i.e., layered double hydroxide (LDH) and lipid-coated calcium phosphate (LCP) and then compared their efficacy for siRNA delivery in T cells, in order to understand which carrier is more efficient in delivering functional programmed cell death protein 1 siRNA (PD-1 siRNA) to suspended T lymphocytes. Both LDH and LCP nanoparticles quickly delivered gene segment to mouse T cell lines (EL4), while the LCP nanoparticles exhibited more cellular uptake and higher PD-1 gene silence efficiency. We further demonstrated that LCP nanoparticles successfully reduced the expression of PD-1 in human ex vivo tumor infiltrating lymphocytes (TILs). Thus, LCP nanoparticles can be used as a better nano-carrier for gene therapy in lymphocytes, especially in regards to TIL-related cancer immunotherapy.
Publisher: American Chemical Society (ACS)
Date: 02-07-2009
DOI: 10.1021/IE900412C
Publisher: American Chemical Society (ACS)
Date: 21-07-2007
DOI: 10.1021/IE0705958
Publisher: Bentham Science Publishers Ltd.
Date: 09-2013
DOI: 10.2174/1381612811319350009
Abstract: Restenosis, the re-narrowing of a blood vessel after removal of atherosclerotic plaque, is a major limitation of surgical treatments for atherosclerosis. Various attempts to prevent or treat restenosis by pharmacological or mechanical approaches have had limited success in clinical trials. Hence, there is wide interest in developing new strategies to prevent or treat restenosis. This review discusses 'a new-generation therapy' that uses functional nanoparticles to effectively deliver active drug molecules. The potential platforms for nanoparticle-based solutions to restenosis include organic (e.g. polymers, liposomes, and proteins) and inorganic nanoparticles (e.g. layered double hydroxides, titanium oxide nanotubes, and magnetic nanoparticles,). Many in vitro and in vivo studies based on these platforms demonstrate the feasibility and potential of using nanoparticle drug delivery systems for preventing or treating restenosis, but as yet few have reached clinical trials. It is suggested that using inorganic nanoparticles to target deliver multi-functional drugs will be a promising approach to preventing or treating restenosis.
Publisher: Bentham Science Publishers Ltd.
Date: 09-2013
DOI: 10.2174/1381612811319350011
Abstract: Exposure of human skin to nanoparticles (NPs) is increasing with the development of nanotechnology and new applications of NPs in medicine. Safety concerns have sparked debate on the capacity of NPs to penetrate through skin and enter into the body. This article attempts to summarize the recent evidence on whether NPs penetrate human skin and the factors that may affect the penetration. Skin structure and penetration mechanisms are reviewed to provide background information. Size, shape, formulation, surface properties and application methods and their effects on skin penetration are specifically discussed. Finally, the relationship between skin penetration and nanotoxicity is reviewed to further emphasise the importance of the research in this area.
Publisher: Elsevier BV
Date: 10-2014
Publisher: American Chemical Society (ACS)
Date: 26-09-2014
DOI: 10.1021/ES502391Y
Abstract: Electroplating sludges, once regarded as industrial wastes, are precious resources of various transition metals. This research has thus investigated the recycling of an electroplating sludge as a novel carbon-doped metal (Fe, Ni, Mg, Cu, and Zn) catalyst, which was different from a traditional carbon-supported metal catalyst, for effective NO selective catalytic reduction (SCR). This catalyst removed >99.7% NO at a temperature as low as 300 °C. It also removed NO steadily (>99%) with a maximum specific accumulative reduced amount (MSARA) of 3.4 mmol/g. Gas species analyses showed that NO removal was accompanied by evolving N2 and CO2. Moreover, in a wide temperature window, the sludge catalyst showed a higher CO2 selectivity (>99%) than an activated carbon-supported metal catalyst. Structure characterizations revealed that carbon-doped metal was transformed to metal oxide in the sludge catalyst after the catalytic test, with most carbon (2.33 wt %) being consumed. These observations suggest that NO removal over the sludge catalyst is a typical SCR where metals/metal oxides act as the catalytic center and carbon as the reducing reagent. Therefore, our report probably provides an opportunity for high value-added utilizations of heavy-metal wastes in mitigating atmospheric pollutions.
Publisher: American Chemical Society (ACS)
Date: 10-2006
DOI: 10.1021/IE060757K
Publisher: Elsevier BV
Date: 02-2013
Publisher: Wiley
Date: 09-08-2021
DOI: 10.1002/EOM2.12132
Abstract: The agrochemical is often applied to enhance the agricultural production. Nanotechnology has advanced agricultural biotechnology in the new “agri‐tech revolution.” Eco‐friendly nanoparticles as carriers can essentially actualize the application of biomolecules as sustainable agrochemicals to increase their biological performance and reduce deterioration of the ecosystem. Particularly, these nanocarrier‐biomolecule hybrids will be ideal for topical applications to enhance the crop yield and quality. This review summarizes the current efforts in developing nanoparticles as carriers of biomolecules for topical delivery. First, the applicable biomolecules for plant growth regulation and the pathogen control are briefly introduced. The criteria and strategies for research and industrial development of nanocarrier‐biomolecule hybrids are then proposed, which better involves both researchers and manufacturers in the beginning for an efficient R& D process. The review further discusses several typical nanoparticle‐biomolecule hybrids as the candidates of next‐generation agrochemicals with advanced scientific merits and high perspectives of industrialization as the conclusion. image
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C1JM11695A
Publisher: Wiley
Date: 16-03-2007
Abstract: In this paper we report the preparation and characterization of [Gd(dtpa)](2-) intercalated layered double hydroxide (LDH) nanomaterials. [Gd(dtpa)](2-) (gadolinium(III) diethylene triamine pentaacetate) was transferred into LDH by anionic exchange. The intercalation of [Gd(dtpa)](2-) into LDH was confirmed by X-ray diffraction for the new phase with the interlayer spacing of 3.5-4.0 nm and by FTIR for the characteristic vibration peaks of [Gd(dtpa)](2-). The morphology of the nanoparticles was influenced by the extent of [Gd(dtpa)](2-) loading, in which the poly-dispersity quality decreased as the [Gd(dtpa)](2-) loading was increased. Compared with the morphology of the original Mg(2)Al-Cl-LDH nanoparticles (hexagonal plate-like sheets of 50-200 nm), the modified LDH-Gd(dtpa) nanoparticles are bar-like with a width of 30-60 nm and a length of 50-150 nm. LDH-Gd(dtpa) was expected to have an increased water proton magnetic resonance relaxivity due to the intercalation of [Gd(dtpa)](2-) into the LDH interlayer that led to slower molecular anisotropic tumbling compared with free [Gd(dtpa)](2-) in solution. Indeed, LDH-nanoparticle suspension containing approximately 1.6 mM [Gd(dtpa)](2-) exhibits a longitudinal proton relaxivity r(1) of approximately 16 mM(-1) s(-1) and a transverse proton relaxivity r(2) of approximately 50 mM(-1) s(-1) at room temperature and a magnetic field of 190 MHz, which represents an enhancement four times (r(1)) and 12 times (r(2)) that of free [Gd(dtpa)](2-) in solution under the same reaction conditions. We have thus tailored LDH-nanoparticles into a novel contrast agent with strong relaxivity, promising for great potential applications in magnetic resonance imaging.
Publisher: Elsevier BV
Date: 02-2018
DOI: 10.1016/J.NANO.2017.11.007
Abstract: Internal bleeding is defined as the loss of blood that occurs inside of a body cavity. After a traumatic injury, hemorrhage accounts for over 35% of pre-hospital deaths and 40% of deaths within the first 24 hours. Coagulopathy, a disorder in which the blood is not able to properly form clots, typically develops after traumatic injury and results in a higher rate of mortality. The current methods to treat internal bleeding and coagulopathy are inadequate due to the requirement of extensive medical equipment that is typically not available at the site of injury. To discover a potential route for future research, several current and novel treatment methods have been reviewed and analyzed. The aim of investigating different potential treatment options is to expand available knowledge, while also call attention to the importance of research in the field of treatment for internal bleeding and hemorrhage due to trauma.
Publisher: Springer Science and Business Media LLC
Date: 12-09-2010
Publisher: Elsevier BV
Date: 09-2011
DOI: 10.1016/J.BIORTECH.2011.03.002
Abstract: Fresh compost leachate was used as a nutrients source to facilitate anaerobic fermentative hydrogen production from glucose inoculated with mixed culture. The optimum condition for hydrogen production was predicted by response surface methodology (RSM). The model showed the maximum cumulative hydrogen volume (469.74 mL) and molar hydrogen yield (1.60 mol H2/mol glucose) could be achieved at 6174.93 mg/L glucose and 3383.20 mg COD/L leachate. According to the predicted optimal condition, four tests were carried out to validate the predicted values and evaluate the leachate's effect on co-fermentation with juice wastewater. A maximum cumulative hydrogen volume of 587.05 ± 15.08 mL was obtained in co-fermentation test, and the molar hydrogen yield reached 2.06 ± 0.06 mol H2/mol glucose. The co-fermentation of fresh leachate and glucose/juice wastewater was a combination of acetic acid and butyric acid type-fermentation. The results demonstrated that leachate can serve as a nutrients source for biohydrogen production.
Publisher: American Chemical Society (ACS)
Date: 12-2017
Abstract: The relation between a catalytic center and the surrounding carbon-oxygen groups influences the catalytic activity in various reactions. However, the impact of this relation on catalysis is usually discussed separately. For the first time, we proved that carbon-oxygen groups increased the reducibility of Fe-C bonds toward NO reduction. Experimentally, we compared the reductive activities of materials with either one or both factors, i.e., carbon-oxygen groups and Fe-C bonds. As a result, graphene oxide-supported Fe (with both factors) showed the best activity, duration of activity, and selectivity. This material reduced 100% of NO to N
Publisher: Elsevier BV
Date: 07-2012
DOI: 10.1016/J.JCIS.2012.03.024
Abstract: The influence of droplet orientation on the flow directed organization of nanoparticles in evaporating nanofluid droplets is important for the efficiency of foliar applied fertilizers and contamination adhesion to the exterior of buildings. The so called "coffee ring" deposit resulting from the evaporation of a sessile nanofluid drop on a hydrophilic surface has received much attention in the literature. Deposits forming on hydrophobic surfaces in the pendant drop position (i.e. hanging drop), which are of importance in foliar fertilizer and exterior building contamination, have received much less attention. In this study, the deposit patterns resulting from the evaporation of water droplets containing silica nanoparticles on hydrophobic surfaces orientated in the sessile or pendant configuration are compared. In the case of a sessile drop the well known coffee ring pattern surrounding a thin nanoparticle layer was formed. A deposit consisting of a thin coffee ring surrounding a bump was formed in the pendant position. A mechanism involving flow induced aggregation at the droplet waist, settling, orientation dependant accumulation within the drop and pinning of the contact line is suggested to explain the findings. Differences in the contact area and adhesion of deposits with surface orientation will affect the efficiency and rainfastness of foliar fertilizers and the cleanliness of building exteriors.
Publisher: Elsevier BV
Date: 2017
Publisher: Elsevier BV
Date: 2013
DOI: 10.1016/J.JCIS.2012.08.003
Abstract: Water-soluble CdTe/CdS quantum dots (QDs) with tuneable emissions were prepared in aqueous solution at pH=6-7 via refluxing and hydrothermal treatment. The resultant CdTe/CdS QDs are stabilized with mercaptosuccinic acid (MSA) and show high fluorescence quantum yields (maximum QY is 84%). Characterization with UV-Vis, PL, XPS, XRD and TEM demonstrates a core (CdTe)-shell (CdS) structure, which leads to high fluorescence quantum yields. The effective protection from CdS shell and MSA enables CdTe QDs to be chemically stable in a pH range of 6-9 and less toxic. These merits make our CdTe/CdS QDs very promising for bio-imaging applications, as exemplified by labelling HEK 293 cells.
Publisher: American Chemical Society (ACS)
Date: 13-03-2008
DOI: 10.1021/IE0716060
Publisher: American Chemical Society (ACS)
Date: 25-05-2018
Abstract: A delivery platform with highly selective permeability through the blood-brain barrier (BBB) is essential for brain disease treatment. In this research, we designed and prepared a novel target nanoplatform, that is, layered double hydroxide (LDH) nanoparticle conjugated with targeting peptide-ligand Angiopep-2 (Ang2) or rabies virus glycoprotein (RVG) via intermatrix bovine serum albumin for brain targeting. In vitro studies show that functionalization with the target ligand significantly increases the delivery efficiency of LDH nanoparticles to the brain endothelial (bEnd.3) cells and the transcytosis through the simulated BBB model, that is, bEnd.3 cell-constructed multilayer membrane. In vivo confocal neuroimaging of the rat's blood-retina area dynamically demonstrates that LDH nanoparticles modified with peptide ligands have shown a prolonged retention period within the retina vessel in comparison with the pristine LDH group. Moreover, Ang2-modified LDH nanoparticles are found to more specifically accumulate in the mouse brain than the control and RVG-modified LDH nanoparticles after 2 and 48 h intravenous injection. All these findings strongly suggest that Ang2-modified LDHs can serve as an effective targeting nanoplatform for brain disease treatment.
Publisher: Elsevier BV
Date: 2013
DOI: 10.1016/J.JCIS.2012.09.033
Abstract: Small interfering RNAs (siRNAs) are a potentially powerful new class of pharmaceutical drugs for many disease. However, the delivery of unprotected siRNAs is ineffective due to their susceptibility to degradation by ubiquitous nucleases under physiological conditions. Layered double hydroxide nanoparticles (LDHs) have been found to be efficient carriers of anionic drugs and nucleic acids. Our previous research has shown that LDHs (with the Z-average particle size of approximately 110 nm) can mediate siRNA delivery in mammalian cells, resulting in gene silencing. However, short double-stranded nucleic acids are mostly adsorbed onto the external surface and not well protected by LDHs. In order to enhance the intercalation of siRNA into the LDH interlayer and the efficiency of subsequent siRNA delivery, we prepared smaller LDHs (with the Z-average particle size of approximately 45 nm) with an engineered non-aqueous method. We demonstrate here that dsDNA/siRNA is more effectively intercalated into these small LDH nanoparticles, more dsDNA/siRNA is transfected into HEK 293T cells, and more efficient silencing of the target gene is achieved using smaller LDHs. Thus, smaller LDH particles have greater potential as a delivery system for the application of RNA interference.
Publisher: Wiley
Date: 30-06-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C1JM12054A
Publisher: American Chemical Society (ACS)
Date: 09-11-2010
DOI: 10.1021/ES102884V
Abstract: Heavy metal ions (Ni(2+), Zn(2+), and Cr(3+)) can be effectively removed from real polynary metal ions-bearing electroplating wastewaters by a carbonation process, with ∼99% of metal ions removed in most cases. The synchronous formation of layered double hydroxide (LDH) precipitates containing these metal ions was responsible for the self-purification of wastewaters. The constituents of formed polynary metals-LDHs mainly depended on the Ni(2+):Zn(2+):Cr(3+) molar ratio in wastewaters. LDH was formed at pH of 6.0-8.0 when the Ni(2+)/Zn(2+) molar ratio ≥ 1 where molar fraction of trivalent metal in the wastewaters was 0.2-0.4, otherwise ZnO, hydrozincite, or amorphous precipitate was observed. In the case of LDH formation, the residual concentration of Ni(2+), Zn(2+), and Cr(3+) in the treated wastewaters was very low, about 2-3, ∼2, and ∼1 mg/L, respectively, at 20-80 °C and pH of 6.0-8.0, indicating the effective incorporation of heavy metal ions into the LDH matrix. Furthermore, the obtained LDH materials were used to adsorb azoic dye GR, with the maximum adsorption amount of 129-134 mg/g. We also found that the obtained LDHs catalyzed more than 65% toluene to decompose at 350 °C under ambient pressure. Thus the current research has not only shown effective recovery of heavy metal ions from the electroplating wastewaters in an environmentally friendly process but also demonstrated the potential utilization of recovered materials.
Publisher: Bentham Science Publishers Ltd.
Date: 11-2013
Publisher: Walter de Gruyter GmbH
Date: 2006
Abstract: This paper briefly reviews the recent progress in using layered double hydroxide (LDH) nanomaterials as cellular delivery agents. The advantages of LDHs as cellular delivery agents are summarized, and the processes of interaction/de-intercalation of anionic drugs (genes) into/from LDH nanoparticles are discussed. Then the cellular delivery of LDH-drug (gene) nanohybrids and subsequent intracellular processes are presumably proposed. At the end, some challenges and remarks for efficient delivery of drugs (genes) via LDH nanoparticles are provided to the best of our knowledge.
Publisher: American Chemical Society (ACS)
Date: 10-02-2005
DOI: 10.1021/CM048085G
Publisher: Springer Science and Business Media LLC
Date: 09-01-2017
Abstract: Topical application of pathogen-specific double-stranded RNA (dsRNA) for virus resistance in plants represents an attractive alternative to transgenic RNA interference (RNAi). However, the instability of naked dsRNA sprayed on plants has been a major challenge towards its practical application. We demonstrate that dsRNA can be loaded on designer, non-toxic, degradable, layered double hydroxide (LDH) clay nanosheets. Once loaded on LDH, the dsRNA does not wash off, shows sustained release and can be detected on sprayed leaves even 30 days after application. We provide evidence for the degradation of LDH, dsRNA uptake in plant cells and silencing of homologous RNA on topical application. Significantly, a single spray of dsRNA loaded on LDH (BioClay) afforded virus protection for at least 20 days when challenged on sprayed and newly emerged unsprayed leaves. This innovation translates nanotechnology developed for delivery of RNAi for human therapeutics to use in crop protection as an environmentally sustainable and easy to adopt topical spray.
Publisher: Elsevier BV
Date: 02-2012
Publisher: Wiley
Date: 14-09-2017
Abstract: Multifunctional nanoparticles for cancer theranosis have been widely explored for effective cancer detection and therapy. In this work, dually functionalised manganese-based layered double hydroxide nanoparticles (Mn-LDH) were examined as an effective anticancer drug/gene delivery system and for T
Publisher: American Chemical Society (ACS)
Date: 20-05-2008
DOI: 10.1021/CM703602T
Publisher: Springer Science and Business Media LLC
Date: 17-10-2017
DOI: 10.1038/S41598-017-13570-2
Abstract: In recent decades, diseases caused by pathogenic Escherichia coli ( E. coli ), enterohaemorrhagic E. coli (EHEC) O26 have been increasingly reported worldwide, which are as severe as those caused by EHEC strain O157:H7 and require effective intervention strategies. Herein, we report the application of clay nanoparticles, i.e. hectorites as effective nano-adjuvants for vaccination against EHEC O26 colonization. We show that medium size HEC (hectorite, around 73~77 nm diameter) is able to induce efficient humoral and cellular immune responses against EHEC antigen - intimin β (IB), which are significantly higher than those triggered by commercially used adjuvants - QuilA and Alum. We also demonstrate that mice immunized with IB adjuvanted with HEC nanoparticles elicit sufficient secretion of mucosal IgA, capable of providing effective protection against EHEC O26 binding to ruminant and human cells. In addition, we demonstrate for the first time that hectorites are able to initiate maturation of RAW 264.7 macrophages, inducing expression of co-stimulatory cytokines at a low nanoparticle concentration (10 μg/mL). Together these data strongly suggest that hectorite with optimized size is a highly efficient vaccine nano-adjuvant.
Publisher: Walter de Gruyter GmbH
Date: 22-07-2021
Abstract: Immunotherapy has made great progress in recent years while most cancer patients cannot benefit from it. Photochemotherapy combination strategy holds great promise for developing novel immunotherapy for the patients bearing immunosuppressive tumors such as colon cancer. In this research, a novel core/shell-structured polydopamine (PDA)-based nanoplatform is constructed to load two Food and Drug Administration (FDA)-approved cytotoxic drugs, i.e. immunostimulatory doxorubicin (Dox) and immunomodulatory curcumin (Cur) to achieve immunostimulatory photochemotherapy of primary colon tumors upon 808 nm near infrared (NIR) irradiation (1 W/cm 2 for 5 min) and subsequent prevention of rechallenged distant colon tumors. The experimental data have shown that PDA-mediated photothermal therapy (PTT) synergized two therapeutic drugs in inducing colon cancer cell death and very efficiently inhibited the primary tumor growth (by ∼92%) at very low doses of therapeutics (0.25, 5, and 30 mg/kg of Dox, Cur, and PDA, respectively). More significantly, the combined photochemotherapy promoted strong adaptive antitumor immune responses and successfully prevented tumorigenesis in the setting of tumor rechallenge model. Our research has thus demonstrated the promising efficacy of this photochemotherapeutic nanoformulation for colon cancer treatment and provided a way to improve immunostimulatory effects of conventional chemotherapeutic drugs.
Publisher: Elsevier BV
Date: 06-2000
Publisher: Springer Science and Business Media LLC
Date: 07-08-2014
Publisher: Springer Science and Business Media LLC
Date: 25-09-2014
Publisher: Wiley
Date: 03-04-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1NR00881A
Abstract: MgAl-layered double hydroxide (LDH) has a similar composition to the commercial aluminum adjuvant, but is highly biocompatible. The excellent adjuvanticity and biocompatibility make LDH the most potential clinical candidate adjuvant.
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0TB00856G
Abstract: A novel fluorescent protein-probe hybrid was devised for ratiometric detection and imaging of intracellular H 2 O 2 with high sensitivity and selectivity.
Publisher: Springer Science and Business Media LLC
Date: 09-10-2014
Publisher: Oxford University Press (OUP)
Date: 22-09-2022
Abstract: RNA interference is triggered in plants by the exogenous application of double-stranded RNA or small interfering RNA (siRNA) to silence the expression of target genes. This approach can potentially provide insights into metabolic pathways and gene function and afford plant protection against viruses and other plant pathogens. However, the effective delivery of biomolecules such as siRNA into plant cells is difficult because of the unique barrier imposed by the plant cell wall. Here, we demonstrate that 40-nm layered double hydroxide (LDH) nanoparticles are rapidly taken up by intact Nicotiana benthamiana leaf cells and by chloroplasts, following their application via infiltration. We also describe the distribution of infiltrated LDH nanoparticles in leaves and demonstrate their translocation through the apoplast and vasculature system. Furthermore, we show that 40-nm LDH nanoparticles can greatly enhance the internalization of nucleic acids by N. benthamiana leaf cells to facilitate siRNA-mediated downregulation of targeted transgene mRNA by & % within 1 day of exogenous application. Together, our results show that 40-nm LDH nanoparticle is an effective platform for delivery of siRNA into intact plant leaf cells.
Publisher: Elsevier BV
Date: 04-2013
Publisher: Elsevier
Date: 2007
Publisher: American Chemical Society (ACS)
Date: 18-05-2020
Abstract: Recently, a widely used antiworm drug albendazole (ABZ) has been recognized as an anticancer drug, while being safe for healthy cells. However, its clinical application is limited due to low water solubility. In this work, we incorporated ABZ into pH-responsive lipid-coated calcium phosphate (LCP) nanoparticles to enhance anticancer efficacy. LCP-ABZ nanoparticles doubled the ABZ solubility with a much quicker release rate in the slightly acidic environment. LCP-ABZ significantly improved cytotoxicity to B16F0 cells and reduced their migration probably through enhanced apoptosis induction
Publisher: American Chemical Society (ACS)
Date: 13-12-2006
DOI: 10.1021/JA056652A
Abstract: Seriously aggregated LDH agglomerates can be dispersed by a hydrothermal treatment into homogeneous stable suspensions that contain LDH particles in the range of 50-300 nm.
Publisher: Wiley
Date: 17-11-2020
Publisher: Elsevier BV
Date: 05-2016
DOI: 10.1016/J.JCIS.2016.02.042
Abstract: MgAl-layered double hydroxide (MgAl-LDH) nanoparticles have great potentials in drug and siRNA delivery. In this work, we used a nanodot-coating strategy to prepare SiO2 dot-coated layered double hydroxide (SiO2@MgAl-LDH) nanocomposites with good dispersibility and controllable size for drug delivery. The optimal SiO2@MgAl-LDH nanocomposite was obtained by adjusting synthetic parameters including the mass ratio of MgAl-LDH to SiO2, the mixing temperature and time. The optimal SiO2@MgAl-LDH nanocomposite was shown to have SiO2 nanodots (10-15nm in diameter) evenly deposited on the surface of MgAl-LDHs (110nm in diameter) with the plate-like morphology and the average hydrodynamic diameter of 170nm. We further employed SiO2@MgAl-LDH nanocomposite as a nanocarrier to deliver methotrexate (MTX), a chemotherapy drug, to the human osteosarcoma cell (U2OS) and found that MTX delivered by SiO2@MgAl-LDH nanocomposite apparently inhibited the U2OS cell growth.
Publisher: Wiley
Date: 19-08-2013
Publisher: American Chemical Society (ACS)
Date: 08-03-1970
DOI: 10.1021/JP056473F
Abstract: CoxMg3-x/Al hydrotalcite-like compounds (where x=0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0) were synthesized by the coprecipitation method and characterized by the XRD and TGA techniques. Incorporation of Co for x=0.0-3.0 gradually decreased the transformation temperature of the hydrotalcites to the corresponding oxides from 444 to 246 degrees C and also decreased the surface area from 162.7 to 21.6 m2/g upon calcination at 800 degrees C for 4 h in air. The resultant oxide was generally composed of a poor MgO phase and a spinel phase, with more spinel phase at higher Co incorporation. The derived oxides were tested as the storage/reduction catalysts for NOx adsorption/desorption. The storage capacity for NOx was highly dependent on the catalyst composition and storage temperature. In general, more NOx was stored at lower temperature (100 degrees C) than that at higher temperature (300 degrees C), and tertiary catalysts (x=0.5-2.5) stored more NOx than binary catalyst (x=0.0 or 3.0). The catalytic conversion of NO to NO2 and the catalytic decomposition of NOx were observed on the tertiary catalysts during NOx adsorption at 300 degrees C, which was highly related to the loading of cobalt. The reducibility of catalysts was determined by TPR experiments, and the reduction of cobalt cations started at 150-200 degrees C in H2. In situ IR spectra of catalysts adsorbing NOx revealed that the major NOx species formed on the catalysts were various kinds of nitrites and nitrates, together with some forms of dimers, such as N2O2(2-) and N2O4 (or NO+NO3-). The storage/reduction mechanism and the function of Co in the mixed oxides are proposed and discussed on the basis of these observations.
Publisher: Elsevier BV
Date: 04-2013
DOI: 10.1016/J.JCIS.2012.11.061
Abstract: Understanding the influences of particle properties of antigen-adjuvant complexes on immunity is crucial in designing highly active adjuvants for new-generation of vaccines. This paper briefly revisits the current opinions on the size-dependent immunity of various adjuvant particles and then comprehensively discusses a few immunity-determining processes that are affected by the antigen-adjuvant particle properties. These include particle size, surface charge, surface hydrophilicity/lipophilicity, and antigen-adjuvant binding strength. Based on current understandings, we hypothesize that a maximum immune response occurs at a certain antigen-adjuvant particle size. This hypothesis clearly explains the paradoxical opinions on the size-dependent immunity and has also been supported by the data reported by several research groups. Finally, we further hypothesize that there is a similar relationship between any immune response and any measureable antigen-adjuvant particle property, and that there is a maximum immune response when all measureable antigen-adjuvant particle properties are optimized. We believe more attention should be paid to this issue when designing and developing effective adjuvants in future research.
Publisher: Elsevier BV
Date: 02-2012
Publisher: MDPI AG
Date: 20-11-2018
DOI: 10.3390/PHARMACEUTICS10040244
Abstract: A major impediment to the long-term in vivo vascular imaging is a lack of suitable probes and contrast agents. Our developed mercaptosuccinic acid (MSA) capped cadmium telluride/cadmium sulfide (CdTe/CdS) ultrasmall quantum dots (QDs) have high fluorescent quantum yield, long fluorescence lifetime and long half-life in blood, allowing high resolution long-term intravital vascular imaging. In this study, we showed that these QDs can be used to visualize the in vivo the vasculature in normal and cancerous livers in mice using multiphoton microscopy (MPM) coupled with fluorescence lifetime imaging (FLIM), with cellular resolution (~1 µm) up to 36 h after intravenous injection. Compared to highly regulated and controlled sinusoids in normal liver tissue, disordered, tortuous, and immature neovessels were observed in tumors. The utilized imaging methods have great potential as emerging tools in diagnosis and monitoring of treatment response in cancer.
Publisher: American Chemical Society (ACS)
Date: 18-03-2011
DOI: 10.1021/JP112031E
Publisher: MDPI AG
Date: 22-05-2014
Publisher: American Chemical Society (ACS)
Date: 17-07-2020
Publisher: Wiley
Date: 07-08-2014
DOI: 10.1002/JBM.A.34888
Abstract: High molecular weight (MW) polyethyleneimine (PEI) has been successfully used for the transfection of a broad variety of cell lines. In contrast to low MW PEI, which exhibits low transfection efficiencies but also low cytotoxicity, high MW PEI-mediated transfection achieves much higher efficiencies but at the cost of cell viability therefore its use in commercial scale transfection and clinical application is limited. In this work we address this problem by constructing biodegradable high MW PEI mimics built from low MW PEI building blocks. The end-groups of small 5-arm star polyethylene glycol (PEG) prepolymers were decorated with linear oligo-ethyleneimine (OEI)/PEI arms of various MW via azomethine linkages. The resultant PEI-PEG-star-copolymers were investigated for their ability to complex plasmid DNA. Polymer/DNA complexes were characterized using techniques such as dynamic light scattering and transmission electron microscopy. Having established their cytotoxicity limits, they were tested as gene delivery vehicles for the transfection of suspension adapted Chinese hamster ovary (CHO-S) cells under serum-free conditions and adherent human embryonic kidney cells (HEK293T) in serum containing medium. Our PEI-PEG-star-copolymers showed a reduced cytotoxicity compared to high MW PEI while maintaining the ability to complex plasmid DNA and transfect mammalian cells, with significant transfection efficiencies. The effects of the optimum parameters on the transfection of mammalian cells using such novel polymers are discussed.
Publisher: Elsevier BV
Date: 29-04-2009
Publisher: Wiley
Date: 31-05-2012
DOI: 10.1002/APJ.599
Start Date: 2008
End Date: 2012
Funder: Australian Research Council
View Funded ActivityStart Date: 2017
End Date: 2019
Funder: Australian Research Council
View Funded ActivityStart Date: 2012
End Date: 2016
Funder: Australian Research Council
View Funded ActivityStart Date: 2010
End Date: 2010
Funder: Australian Research Council
View Funded ActivityStart Date: 2005
End Date: 2007
Funder: Australian Research Council
View Funded ActivityStart Date: 2012
End Date: 2014
Funder: Australian Research Council
View Funded ActivityStart Date: 2009
End Date: 2011
Funder: Australian Research Council
View Funded ActivityStart Date: 2012
End Date: 12-2015
Amount: $360,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 2017
End Date: 12-2020
Amount: $325,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 2013
End Date: 01-2017
Amount: $787,936.00
Funder: Australian Research Council
View Funded ActivityStart Date: 2005
End Date: 12-2008
Amount: $495,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 2008
End Date: 06-2014
Amount: $1,330,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 01-2019
End Date: 12-2022
Amount: $450,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 01-2009
End Date: 02-2014
Amount: $510,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 2010
End Date: 12-2010
Amount: $495,000.00
Funder: Australian Research Council
View Funded ActivityStart Date: 08-2020
End Date: 08-2025
Amount: $4,787,259.00
Funder: Australian Research Council
View Funded Activity