ORCID Profile
0000-0002-9265-6941
Current Organisations
University of Ibadan
,
University of Manchester
,
CSIRO
,
CSIRO Black Mountain Laboratories
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Publisher: Oxford University Press (OUP)
Date: 26-04-2011
DOI: 10.1093/JAC/DKR162
Abstract: To determine the complete nucleotide sequence of the novel Johannesburg β-lactamase-encoding gonococcal plasmid (pEM1) and to determine the strain relatedness of Johannesburg plasmid-containing penicillinase-producing Neisseria gonorrhoeae (PPNG) by molecular typing. Eleven PPNG isolates containing the Johannesburg β-lactamase-encoding plasmid were previously identified among gonococci isolated from men with urethral discharge attending a clinic in Alexandra (Johannesburg) using a PCR assay. DNA sequence-based characterization of one such plasmid was performed to determine its relatedness to the prototype Asia plasmid. The 11 PPNG isolates containing the Johannesburg plasmid and 105 other clinical gonococci isolates were typed using N. gonorrhoeae multi-antigen sequence typing (NG-MAST). Plasmid pEM1 was determined to comprise 4865 bp and to be a deletion derivative of the prototype Asia plasmid with a unique 2560 bp deletion in the non-TnA region. NG-MAST genotyping demonstrated a significant association between sequence type (ST) 502, or other closely related STs, and the Johannesburg plasmid-containing PPNG (P < 0.0001). Sequencing of a novel β-lactamase-encoding plasmid (pEM1) found in PPNG isolates in Johannesburg shows it to be a deletion derivative of the prototype Asia plasmid, the deletion most likely arising as a result of DNA rearrangements. The majority of Johannesburg plasmid-containing PPNG isolates were, or were very closely related to, ST502.
Publisher: CSIRO: Water for a Healthy Country National Research Flagship
Date: 2008
Publisher: CSIRO
Date: 2020
DOI: 10.25919/FPNF-W949
Publisher: CSIRO
Date: 2012
Publisher: Ovid Technologies (Wolters Kluwer Health)
Date: 04-2011
Publisher: Australia, CSIRO
Date: 2008
Publisher: CSIRO
Date: 2021
DOI: 10.25919/0VNY-QF35
Publisher: CSIRO client report
Date: 2017
Publisher: Springer International Publishing
Date: 2015
Publisher: CSIRO
Date: 2020
DOI: 10.25919/GVCM-ZG09
Publisher: CSIRO
Date: 2018
Publisher: CSIRO
Date: 2020
Location: United Kingdom of Great Britain and Northern Ireland
Location: United Kingdom of Great Britain and Northern Ireland
Location: Australia
No related grants have been discovered for David Lemon.