ORCID Profile
0000-0001-7867-1224
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Publisher: MDPI AG
Date: 30-03-2023
DOI: 10.3390/MI14040770
Abstract: Cell metabolite detection is important for cell analysis. As a cellular metabolite, lactate and its detection play an important role in disease diagnosis, drug screening and clinical therapeutics. This paper reports a microfluidic chip integrated with a backflow prevention channel for cell culture and lactate detection. It can effectively realize the upstream and downstream separation of the culture chamber and the detection zone, and prevent the pollution of cells caused by the potential backflow of reagent and buffer solutions. Due to such a separation, it is possible to analyze the lactate concentration in the flow process without contamination of cells. With the information of residence time distribution of the microchannel networks and the detected time signal in the detection chamber, it is possible to calculate the lactate concentration as a function of time using the de-convolution method. We have further demonstrated the suitability of this detection method by measuring lactate production in human umbilical vein endothelial cells (HUVEC). The microfluidic chip presented here shows good stability in metabolite quick detection and can work continuously for more than a few days. It sheds new insights into pollution-free and high-sensitivity cell metabolism detection, showing broad application prospects in cell analysis, drug screening and disease diagnosis.
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8LC01057A
Abstract: A liquid marble is a microliter-sized droplet coated with hydrophobic powder.
Publisher: American Chemical Society (ACS)
Date: 09-04-2020
Publisher: MDPI AG
Date: 23-03-2023
DOI: 10.3390/PR11040983
Abstract: Microfluidics, as one of the most valuable analytical technologies of this century, has played an important role in various fields. Particularly, out-of-channel microfluidics, often referred to as open microfluidics (OMF) has recently drawn wide research attention among scholars for its great potential in convenient manual intervention. Much recent research has been geared toward bare droplets and particle-armed droplets (namely liquid marbles, LMs), which could serve as independent systems in OMF. Their versatile applications include but are not limited to nanomaterials preparation, energy harvesting, cell culture and environment monitoring. These applications are mainly attributed to the excellent independence, low reagent consumption and short reaction time of separate droplets and LMs. In addition, more operation features, such as erse handling options, flexible controllability and high precision, further enable droplets and LMs carrying small liquid biochemical s les to be manipulated in an open environment freely. Considering the emergence of important research on bare droplets and LMs, this paper systematically reviews the state of the art in the fundamentals and manipulation of the two novel platforms under the frame of OMF. First, the intrinsic property of bare droplets on solid substrates, especially on superhydrophobic ones, is discussed, followed by the formation mechanism of nonwetting LMs and the effect of coating particles on LMs’ performance. Then, friction obstacles and actuation principles raised in driving droplets and LMs are further analyzed theoretically. Subsequently, several classical types of manipulation tasks for both droplets and LMs, namely transportation, coalescence, mixing and splitting, are discussed with a focus on key techniques to accomplish the tasks aforementioned. Finally, the fundamental and manipulation similarities and differences between bare droplets and LMs are summarized and future developments towards droplet- or LM-based microreactors and microsensors are recommended accordingly.
Publisher: Royal Society of Chemistry (RSC)
Date: 2023
DOI: 10.1039/D3LC00310H
Abstract: A novel microfluidic technology integrating capturing, elasticity measurement, and printing of single cells based on the precise regulation of pressure across an array of U-shaped microtraps.
Publisher: AIP Publishing
Date: 05-2019
DOI: 10.1063/1.5094522
Abstract: Liquid marble is a recently emerging digital microfluidic platform with a wide range of applications. Conventional liquid marbles are synthesized by coating liquid droplets with a thin layer of hydrophobic powder. Existing and emerging applications of liquid marbles require a contamination-free synthesis of liquid marbles with a high degree of reproducibility of their volume. Despite this requirement, the synthesis of liquid marbles has been still carried out manually. Manual production of liquid marbles leads to inconsistent volume and the possibility of contamination. The synthesis of liquid marbles with submicroliter volume is difficult to achieve and prone to large errors. This paper discusses the design and development of the first automated on-demand liquid marble generator with submicroliter capability. The device utilizes electrohydrodynamic pulling of liquid droplets on to a hydrophobic powder bed and subsequently coats them with the hydrophobic powder to synthesize liquid marbles of a desired volume.
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8LC00990B
Abstract: We critically evaluate the state of the art of the development of digital polymerase chain reaction systems.
Publisher: MDPI AG
Date: 07-07-2020
DOI: 10.3390/PR8070793
Abstract: The need for miniaturised reaction systems has led to the development of various microreactor platforms, such as droplet-based microreactors. However, these microreactors possess inherent drawbacks, such as rapid evaporation and difficult handling, that limit their use in practical applications. Liquid marbles are droplets covered with hydrophobic particles and are a potential platform that can overcome the weaknesses of bare droplets. The coating particles completely isolate the interior liquids from the surrounding environment, thus conveniently encapsulating the reactions. Great efforts have been made over the past decade to demonstrate the feasibility of liquid marble-based microreactors for chemical and biological applications. This review systemically summarises state-of-the-art implementations of liquid marbles as microreactors. This paper also discusses the various aspects of liquid marble-based microreactors, such as the formation, manipulation, and future perspectives.
Publisher: MDPI AG
Date: 16-10-2022
DOI: 10.3390/BIOS12100878
Abstract: The micropillar array electrode (µAE) has been widely applied in microchip-based electrochemical detection systems due to a large current response. However, it was found that lifying the current through further adjusting geometrical parameters is generally hindered by the shielding effect. To solve this problem, a bio-inspired micropillar array electrode (bµAE) based on the microfluidic device has been proposed in this study. The inspiration is drawn from the structure of leatherback sea turtles’ mouths. By deforming a μAE to rearrange the micropillars on bilateral sides of the microchannel, the contact area between micropillars and analytes increases, and thus the current is substantially improved. A numerical simulation was then used to characterize the electrochemical performance of bµAEs. The effects of geometrical and hydrodynamic parameters on the current of bµAEs were investigated. Moreover, a prototypical microchip integrated with bµAE was fabricated for detailed electrochemical measurement. The chrono erometry measurements were conducted to verify the theoretical performance of bµAEs, and the results suggest that the experimental data are in good agreement with those of the simulation model. This work presents a novel bµAE with great potential for highly sensitive electrochemical detection and provides a new perspective on the efficient configuration of the µAE.
Publisher: Elsevier BV
Date: 10-2018
Publisher: American Chemical Society (ACS)
Date: 15-08-2023
Publisher: American Chemical Society (ACS)
Date: 25-06-2020
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D0LC01290D
Abstract: Liquid marbles are microliter-sized non-wetting droplets. Their versatility makes them an attractive digital microfluidics platform. This paper provides state-of-the-art discoveries in the physics of liquid marbles and their applications.
Publisher: American Chemical Society (ACS)
Date: 07-12-2022
Publisher: MDPI AG
Date: 26-02-2020
DOI: 10.3390/MI11030242
Abstract: Over the last three decades, the protocols and procedures of the DNA lification technique, polymerase chain reaction (PCR), have been optimized and well developed. However, there have been no significant innovations in processes for s le dispersion for PCR that have reduced the amount of single-use or unrecyclable plastic waste produced. To address the issue of plastic waste, this paper reports the synthesis and successful use of a core-shell bead microreactor using photopolymerization of a composite liquid marble as a dispersion process. This platform uses the core-shell bead as a simple and effective s le dispersion medium that significantly reduces plastic waste generated compared to conventional PCR processes. Other improvements over conventional PCR processes of the novel dispersion platform include increasing the throughput capability, enhancing the performance and portability of the thermal cycler, and allowing for the contamination-free storage of s les after thermal cycling.
Publisher: Wiley
Date: 08-02-2023
Abstract: Micromixers play an important role in the micro total analysis systems (µTAS) that require rapid and effective mixing. However, current micromixers are usually designed to meet the need for mixing at limited Reynolds numbers. Herein, this paper presents a high‐performance 3D micromixer with helical elements over wide Reynolds numbers to achieve efficient mixing and has numerically investigated flow patterns and mixing characteristics accordingly. A coupled numerical model is built to analyze the flow pattern, mixing behavior, residence time distribution (RTD), and mixing performance of the 3D micromixer. Helical elements inside could greatly enhance a secondary flow and induce chaotic advection around. Dean vortices are observed in the micromixer, enormously shortening the RTD and promoting the related mixing effect. Furthermore, the effects of various geometric parameters are systematically investigated to optimize the performance of this 3D micromixer. The optimized micromixer shows excellent mixing ability over wide Reynolds numbers ranging from 0.01 to 2333.3, with an efficiency of over 94%. In addition, the numerical results are proved well consistent with analytical and experimental data correspondingly. Therefore, this work would potentially expand the use scope of 3D micromixers and provide a constructive strategy to develop essential parts involving the mixing or reacting process in µTAS.
Publisher: AIP Publishing
Date: 28-01-2019
DOI: 10.1063/1.5079438
Abstract: Liquid marbles can be characterized using elastic solid models consisting of a liquid surrounded by a soft solid membrane. The elastic properties of liquid marbles determine the amount of compression under a given external force. This is an important property as the elasticity of liquid marbles determines their morphology under a given stress. We show that the stress-strain relationship of liquid marbles can be described by σ*Bo=0.6[1/(1−εhro)2−1], where Bo is the Bond number, σ* is the normalised stress, and εhr0 is the strain measured with respect to the equivalent radius of the liquid marble. This stress-strain relationship could pave the way for the development of microfluidic devices with robust liquid marbles.
Publisher: Springer Science and Business Media LLC
Date: 03-06-2019
Publisher: American Physical Society (APS)
Date: 18-04-2019
Publisher: American Physical Society (APS)
Date: 02-01-2020
Publisher: MDPI AG
Date: 20-11-2017
DOI: 10.3390/MI8110336
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8SM00121A
Abstract: We determined the critical condition for the coalescence of two identical liquid marbles through collision.
Publisher: Springer Science and Business Media LLC
Date: 21-11-2018
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9LC00676A
Abstract: Liquid marbles can serve as a biochemical reactor for the polymerase chain reaction, eliminating the conventional single use plastic reaction vial.
Publisher: MDPI AG
Date: 07-08-2020
DOI: 10.3390/MI11080761
Abstract: Multiplex polymerase chain reaction (PCR) is an effective tool for simultaneous detection of target genes. Nevertheless, their use has been restricted due to the intrinsic interference between primer pairs. Performing several single PCRs in an array format instead of a multiplex PCR is a simple way to overcome this obstacle. However, there are still major technical challenges in designing a new generation of single PCR microreactors with a small s le volume, rapid thermal cycling, and no evaporation during lification. We report a simple and robust core-shell bead array for a series of single lifications. Four core-shell beads with a polymer coating and PCR mixture were synthesized using liquid marble formation and subsequent photo polymerization. Each bead can detect one target gene. We constructed a customised system for thermal cycling of these core-shell beads. Phylogrouping of the E. coli strains was carried out based on the fluorescent signal of the core-shell beads. This platform can be a promising alternative for multiplex nucleic acid analyses due to its simplicity and high throughput. The platform reported here also reduces the cycling time and avoids evaporation as well as contamination of the s le during the lification process.
Publisher: MDPI AG
Date: 30-11-2022
DOI: 10.3390/BIOS12121100
Abstract: Separation and clonal culture and growth kinetics analysis of target cells in a mixed population is critical for pathological research, disease diagnosis, and cell therapy. However, long-term culture with time-lapse imaging of the isolated cells for clonal analysis is still challenging. This paper reports a microfluidic device with four-level filtration channels and a pneumatic microvalve for size sorting and in situ clonal culture of single cells. The valve was on top of the filtration channels and used to direct fluid flow by membrane deformation during separation and long-term culture to avoid shear-induced cell deformation. Numerical simulations were performed to evaluate the influence of device parameters affecting the pressure drop across the filtration channels. Then, a droplet model was employed to evaluate the impact of cell viscosity, cell size, and channel width on the pressure drop inducing cell deformation. Experiments showed that filtration channels with a width of 7, 10, 13, or 17 μm successfully sorted K562 cells into four different size ranges at low driving pressure. The maximum efficiency of separating K562 cells from media and whole blood was 98.6% and 89.7%, respectively. Finally, the trapped single cells were cultured in situ for 4–7 days with time-lapse imaging to obtain the lineage trees and growth curves. Then, the time to the first ision, variation of cell size before and after ision, and cell fusion were investigated. This proved that cells at the G1 and G2 phases were of significantly distinct sizes. The microfluidic device for size sorting and clonal expansion will be of tremendous application potential in single-cell studies.
Publisher: Elsevier BV
Date: 10-2021
No related grants have been discovered for Jing Jin.