ORCID Profile
0000-0002-5111-4097
Current Organisations
Marine Bioproducts Cooperative Research Centre
,
Flinders University
,
Dalian Institute of Chemical Physics
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Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Organic Chemistry | Organic Green Chemistry | Bioprocessing, Bioproduction and Bioproducts | Analytical Spectrometry | Biochemistry and Cell Biology | Macromolecular and Materials Chemistry | Colloid and Surface Chemistry | Polymerisation Mechanisms | Characterisation Of Macromolecules | Physical Organic Chemistry | Biochemistry and Cell Biology not elsewhere classified | Industrial Chemistry | Animal Developmental and Reproductive Biology | Industrial Biotechnology | Crop and Pasture Biochemistry and Physiology | Agricultural Marine Biotechnology |
ANZSRC Socio-Economic Objective (SEO)
Expanding Knowledge in the Biological Sciences | Paints | Nutraceuticals and Functional foods | Inorganic Industrial Chemicals | Organic Industrial Chemicals (excl. Resins, Rubber and Plastics) | Expanding Knowledge in the Medical and Health Sciences | Scientific instrumentation | Management of Solid Waste from Plant Production | Ceramics, glass and industrial mineral products not elsewhere classified | Expanding Knowledge in the Chemical Sciences | Expanding Knowledge in Engineering | Expanding Knowledge in the Agricultural and Veterinary Sciences | Marine Flora, Fauna and Biodiversity
Related Links
Publications
Modified green synthesis of Fe3 O4 @SiO2 nanoparticles for pH responsive drug release
Publisher: Elsevier BV
Date: 07-2020
Green magnetic nanomaterial as antibiotic release vehicle
Publisher: Elsevier BV
Date: 2021
Economic Feasibility Analysis of the Industrial Production of Fish Protein Hydrolysates using Conceptual Process Simulation Software
Publisher: OMICS Publishing Group
Date: 2015
Characterization of anthocyanic vacuolar inclusions in Vitis Vinifera L. cell suspension cultures
Publisher: Springer Science and Business Media LLC
Date: 18-03-2010
DOI: 10.1007/S00425-010-1139-4
Abstract: Anthocyanic vacuolar inclusions (AVIs) are intra-vacuolar structures capable of concentrating anthocyanins and are present in over 50 of the highest anthocyanin-accumulating plant species. Presence of AVIs alters pigment intensity, total anthocyanin levels, pigment hue and causes bathochromic shifts in a spatio-temporal manner within various flowers, vegetables and fruits. A year-long study on Vitis vinifera cell suspension cultures found a strong correlation between AVI prevalence and anthocyanin content, but not the number of pigmented cells, growth rate or stilbene content. Furthermore, enhancement of the prevalence of AVIs and anthocyanins was achieved by treatment of V. vinifera cell suspension cultures with sucrose, jasmonic acid and white light. A unique autofluorescence of anthocyanins was used to demonstrate microscopically that AVIs proceed from the cytosol across the tonoplast and were able to coalesce intravacuolarly, with fewer, larger AVIs predominating as cells mature. Purification and characterisation of these bodies were performed, showing that they were dense, highly organic structures, with a lipid component indicative of membrane-encasement. These purified AVIs were also shown to comprise long-chain tannins and possessed an increased affinity for binding acylated anthocyanins, though no unique protein component was detected.
Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture
Publisher: Elsevier BV
Date: 06-2014
Culturable Actinobacteria from the Marine Sponge Hymeniacidon perleve: Isolation and Phylogenetic Diversity by 16S rRNA gene-RFLP Analysis
Publisher: Springer Science and Business Media LLC
Date: 27-07-2006
DOI: 10.1007/S10482-006-9070-1
Abstract: A total of 106 actinobacteria associated with the marine sponge Hymeniacidon perleve collected from the Yellow Sea, China were isolated using eight different media. The number of species and genera of actinobacteria recovered from the different media varied significantly, underlining the importance of optimizing the isolation conditions. The phylogenetic ersity of the actinobacteria isolates was assessed using 16S rRNA gene lification-restriction fragment length polymorphism (RFLP) analysis of the 106 strains with different morphologies. The RFLP fingerprinting of selected strains by HhaI-digestion of the 16S rRNA genes resulted in 11 different patterns. The HhaI-RFLP analysis gave good resolution for the identification of the actinobacteria isolates at the genus level. A phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates belonged to seven genera of culturable actinobacteria including Actinoalloteichus, Micromonospora, Nocardia, Nocardiopsis, Pseudonocardia, Rhodococcus, and Streptomyces. The dominant genus was Streptomyces, which represented 74% of the isolates. Three of the strains identified are candidates for new species.
Altered mRNA Expression Related to the Apoptotic Effect of Three Xanthones on Human Melanoma SK-MEL-28 Cell Line
Publisher: Hindawi Limited
Date: 2013
DOI: 10.1155/2013/715603
Abstract: We previously demonstrated that α -mangostin, γ -mangostin, and 8-deoxygartanin have significant cytotoxic effects on human melanoma SK-MEL-28 cell line. The current study revealed the underlying mechanisms. α -Mangostin (7.5 μ g/mL) activated caspase activity, with a 3-fold and 4-fold increased caspase 8 and 9 activity, respectively. The molecular mechanisms were investigated by qRT-PCR for mRNA related to cell cycle arrest in G 1 phase ( p 21 WAF 1 and cyclin D1), apoptosis (cytochrome C, Bcl-2, and Bax), and survival pathways (Akt1, NF κ B, and I κ B α ). α -Mangostin significantly upregulated mRNA expression of cytochrome C and p 21 WAF 1 and downregulated that of cyclin D1, Akt1, and NF κ B. γ -Mangostin significantly downregulated mRNA expression of Akt1 and NF κ B and upregulated p 21 WAF 1 and I κ B α . 8-Deoxygartanin significantly upregulated the mRNA expression of p 21 WAF 1 and downregulated that of cyclin D1 and NF κ B. The three xanthones significantly inhibited the mRNA expression of the BRAF V600E mutation. Moreover, α -mangostin and γ -mangostin significantly downregulated Akt phosphorylation at Ser473. In conclusion, the three xanthones induced an inhibitory effect on SK-MEL-28 cells by modulating the molecular targets involved in the apoptotic pathways.
New Frontiers in Sponge Science – the 2013 Fremantle Sponge Conference
Publisher: Cambridge University Press (CUP)
Date: 12-02-2016
In Vivo and Clinical Studies of Sea Cucumber-Derived Bioactives for Human Health and Nutrition From 2012-2021
Publisher: Frontiers Media SA
Date: 18-07-2022
DOI: 10.3389/FMARS.2022.917857
Abstract: Sea cucumbers are amongst the highest value seafoods available commercially, especially in the south-east Asian region, primarily due to their nutritional and health benefits as applied in Traditional Chinese Medicine. While the majority of studies for nutritional products derived from sea cucumber compounds have been conducted in vitro , the number of in vivo and evidence-based human clinical studies are limited. This review has critically assessed the advances in in vivo and clinical studies of sea cucumber-derived bioactives (both extracts and compounds) via a comprehensive literature research on papers published in the last ten years from 2012-2021. Sea cucumber-derived compounds were reported to have the following activities: anticancer, antihyperlipidemic, antihyperglycemic, anti-inflammatory, anticoagulant/antithrombotic, antioxidant, and antihypertension, immunomodulatory, wound healing and as treatments for Alzheimer’s and Parkinson’s diseases. These active compounds include triterpene glycosides (saponins), fucosylated chondroitin sulfate (FCS), cerebrosides, glycosaminoglycan, fucoidan, phospholipids, polysaccharides, peptides, long-chain bases, Frondanol A5, acid mucopolysaccharide, and phosphatidylcholines. Gaps, challenges and future directions have been identified and discussed separately to progress different areas of research and to further scientific validation, development and application of sea cucumbers for human health and nutritional products.
Upsized Vortex Fluidic Device Enhancement of Mechanical Properties and the Microstructure of Biomass-Based Biodegradable Films
Publisher: American Chemical Society (ACS)
Date: 21-10-2021
Functions, applications and production of protein hydrolysates from fish processing co-products (FPCP)
Publisher: Elsevier BV
Date: 2013
A benzoxazole compound as a novel MEK inhibitor for the treatment of RAS/RAF mutant cancer
Publisher: Wiley
Date: 28-01-2019
DOI: 10.1002/IJC.32119
Abstract: Mutations in RAS/RAF occur in large portion of malignancies and are associated with aggressive clinical behaviors and poor prognosis. Therefore, we developed a novel benzoxazole compound (KZ-001) as a highly potent and selective MEK 1/2 inhibitor. Our efforts were focused on enhancing the activity of the known MEK inhibitor AZD6244 and overcoming the shortcomings existing in current MEK inhibitors. Here we show that compound KZ-001 exhibits approximately 30-fold greater inhibition against BRAF- and KRAS-mutant tumor cells than that of AZD6244. These results were also demonstrated using in vivo xenograft models. Furthermore, pharmacokinetics (PK) analysis was performed for KZ-001, and this compound showed good orally bioavailability (28%) and exposure (AUC
Deoxyuridines from the Marine Sponge Associated Actinomycete Streptomyces microflavus
Publisher: MDPI AG
Date: 26-04-2011
DOI: 10.3390/MD9050690
Microfluidic Devices in Fabricating Nano or Micromaterials for Biomedical Applications
Publisher: Wiley
Date: 21-10-2019
Characterisation of processing wastes of Atlantic Salmon (Salmo salar) and Yellowtail Kingfish (Seriola lalandi) harvested in Australia
Publisher: Wiley
Date: 28-06-2011
Efficient bioremediation of total organic carbon (TOC) in integrated aquaculture system by marine sponge Hymeniacidon perleve
Publisher: Wiley
Date: 02-2007
DOI: 10.1002/BIT.21352
Abstract: The aim of this study is to investigate the potential of using marine sponge Hymeniacidon perleve to remove total organic carbon (TOC) in integrated aquaculture ecosystems. In sterilized natural seawater (SNSW) with different concentrations of TOC, H. perleve removed approximately 44-61% TOC during 24 h, with retention rates of ca. 0.19-1.06 mg/h .g-fresh sponge, however no particulate selectivity was observed. The highest initial TOC concentration, in which about 2.7 g fresh sponges could remove TOC effectively in 0.5-L SNSW, is 214.3-256.9 mg/L. The highest capacity of TOC removal and clearance rate (CR) by H. perleve is ca. 25.50 mg-TOC/g-fresh sponge and 7.64 mL/h . g-fresh sponge within 24 h, respectively. Until reaching the highest TOC removal capacity, the TOC removal capacity and clearance rate of H. perleve increased with initial TOC concentration, and dropped dramatically thereafter. After reaching the highest removal capacity, H. perleve could only remove relatively lower TOC concentration in seawater in subsequent run. The TOC removal kinetics in SNSW by H. perleve fitted very well with a S-shaped curve and a Logistic model equation (R(2) = 0.999). In different volumes of SNSW with a fixed initial TOC concentration, the weight/volume ratio of sponge biomass and SFNSW was optimized at 1.46 g-fresh sponge/1-L SNSW to achieve the maximum TOC removal. When co-cultured with marine fish Fugu rubripes for 15 days, H. perleve removed TOC excreted by F. rubripes with similar retention rates of ca. 0.15 mg/h . g-fresh sponge, and the sponge biomass increased by 22.8%.
Stability of phenolic compounds and antioxidant capacity of concentrated mulberry juice-enriched dried-minced pork slices during preparation and storage
Publisher: Elsevier BV
Date: 07-2018
Optimal Light Regime for the Cultivation of Transgenic Laminaria japonica Gametophytes in a Bubble-column Bioreactor
Publisher: Springer Science and Business Media LLC
Date: 09-2005
DOI: 10.1007/S10529-005-0938-3
Abstract: Fluctuating light intensity had a more significant impact on growth of gametophytes of transgenic Laminaria japonica in a 2500 ml bubble-column bioreactor than constant light intensity. A fluctuating light intensity between 10 and 110 microE m(-2) s(-1), with a photoperiod of 14 h:10 h light:dark, was the best regime for growth giving 1430 mg biomass l(-1).
Potential of the marine sponge Hymeniacidon perleve as a bioremediator of pathogenic bacteria in integrated aquaculture ecosystems
Publisher: Wiley
Date: 09-02-2006
DOI: 10.1002/BIT.20823
Abstract: The aim of this article is to investigate the potential of using sponges as a bioremediator to remove pathogenic bacteria in integrated aquaculture ecosystems. Using the inter-tidal marine sponge Hymeniacidon perleve as a model system, the ability of removing the most common pathogens Escherichia coli and Vibrio anguillarum II in aquaculture waters was screened in laboratory tests. In sterilized natural seawater (SNSW) supplemented with E. coli at (7.0-8.3) x 10(6) cells/mL, H. perleve can remove an average 96% of E.coli within 10.5 h at a filter rate of ca. (7.53-8.03) x 10(7) cells/h x g of fresh sponge in two independent tests. Despite the removal efficiency and filter rate are similar the clearance rates (CR) vary significantly among in idual sponge specimens and between two batches. For the tests on V. anguillarum II in SNSW, about 1.5 g fresh sponges can keep the pathogen growth under control at a lower initial density 3.6 x 10(4) cells/mL of 200 mL water volume. Further tests were done for 24 h using about 12 g fresh sponge in 2-L actual seawater collected from two aquaculture sites that have ca. eightfold difference in pathogenic bacteria load. The concentrations of E. coli, Vibrio, and total bacteria at 24 h in treatment groups were markedly lower, at about 0.9%, 6.2%-34.5%, and 13.7%-22.5%, respectively, of those in the control. Using a fluoresce stain 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate, E. coli, and V. anguillarum II cells were stained and fed to sponges in two independent tests. The confocal microscope observation confirmed that the sponges filtering-retained and digested these bacteria by phagocytosis.
Halichondria sulfonic acid, a new HIV-1 inhibitory guanidino-sulfonic acid, and halistanol sulfate isolated from the marine sponge Halichondria rugosa Ridley & Dendy
Publisher: Informa UK Limited
Date: 10-2006
DOI: 10.1080/14786410600879748
Abstract: A new sulfur-containing guanidino derivative, halichondria sulfonic acid (1) showing anti-HIV-1 activity, and halistanol trisulfate (2) with anti-tumor activity have been isolated from the marine sponge Halichondria rugosa Ridley & Dendy collected in the Chinese Southern Sea. The structure of 1 was elucidated by analysis of spectroscopic and crystal data.
Purification and characterization of a dehalogenase from Pseudomonas stutzeri DEH130 isolated from the marine sponge Hymeniacidon perlevis
Publisher: Springer Science and Business Media LLC
Date: 31-03-2013
DOI: 10.1007/S11274-013-1340-2
Abstract: 2-haloacid dehalogenases are enzymes that are capable of degrading 2-haloacid compounds. These enzymes are produced by bacteria, but so far they have only been purified and characterized from terrestrial bacteria. The present study describes the purification and characterization of 2-haloacid dehalogenase from the marine bacterium Pseudomonas stutzeri DEH130. P. Stutzeri DEH130 contained two kinds of 2-haloacid dehalogenase (designated as Dehalogenase I and Dehalogenase II) as detected in the crude cell extract after ammonium sulfate fractionation. Both enzymes appeared to exhibit stereo-specificity with respect to substrate. Dehalogenase I was a 109.9-kDa enzyme that preferentially utilized D-2-chloropropropionate and had optimum activity at pH 7.5. Dehalogenase II, which preferentially utilized L-2-chloropropionate, was further purified by ion-exchange chromatography and gel filtration. Purified Dehalogenase II appeared to be a dimeric enzyme with a subunit of 26.0-kDa. It had maximum activity at pH 10.0 and a temperature of 40 °C. Its activity was not inhibited by DTT and EDTA, but strongly inhibited by Cu²⁺, Zn²⁺, and Co²⁺. The K(m) and V(max) for L-2-chloropropionate were 0.3 mM and 23.8 μmol/min/mg, respectively. Its substrate specificity was limited to short chain mono-substituted 2-halocarboxylic acids, with no activity detected toward fluoropropionate and monoiodoacetate. This is the first report on the purification and characterization of 2-haloacid dehalogenase from a marine bacterium.
Molecular cloning of partial 14-3-3 genes in the marine sponge Hymeniacidon perleve and its role in differentiating infectious and non-infectious bacteria
Publisher: Springer Science and Business Media LLC
Date: 09-2012
Astragalus Shiitake—A Novel Functional Food with High Polysaccharide Content and Anti-Proliferative Activity in a Colorectal Carcinoma Cell Line
Publisher: MDPI AG
Date: 02-06-2022
DOI: 10.3390/NU14112333
Abstract: The chemical and nutritional constituents of mushrooms can alter significantly when grown on different substrates. Based on this fact, an approach was made to cultivate a new type of mushroom, Hengshan Astragalus Shiitake, by growing Shiitake mushrooms on beds supplemented with the roots of an edible herbal plant, Astragalus membranaceus. In this study, three green extraction techniques, including microwave-enzyme assisted (MEA), ultrasound-enzyme assisted (UEA) and microwave-ultrasound-enzyme assisted (MUEA) extractions, were used to compare both the yield and antiproliferative activity of the polysaccharide-rich extracts (PREs) from HAS in human colorectal carcinoma cells (HCT 116). Both HAS-A and HAS-B extracts contain significantly higher amounts of polysaccharides when compared to the control (Shiitake extract), regardless of the extraction methods. The PREs from HAS-B have significantly higher anti-proliferative activity in HCT 116 compared to the control when using the UEA extraction method. Our findings demonstrate that HAS-B can become a novel functional food with anti-proliferative activities and the optimization of UEA extraction would help to develop new active extract-based health products.
Chiral Self-Assembly of Designed Amphiphiles: Influences on Aggregate Morphology
Publisher: American Chemical Society (ACS)
Date: 30-07-2013
DOI: 10.1021/LA401987Y
Abstract: A series of novel hiphiles were designed for self-assembly into chiral morphologies, the hiphiles consisting of a glutamic acid (Glu) headgroup connected through an 11-carbon alkoxy chain to a diphenyldiazenyl (Azo) group and terminated with a variable length alkyl chain (R-Azo-11-Glu, where R denotes the number of carbons in the distal chain). TEM imaging of hiphile aggregates self-assembled from heated, methanolic, aqueous solution showed that chiral order, expressed as twisted ribbons, helical ribbons, and helically based nanotubes, increased progressively up to a distal chain length containing eight carbons, and then decreased with further increases in distal chain length. TEM and CD showed that the chiral aggregations of single enantiomers were influenced by the molecular chirality of the headgroup. However, the assembly of D,L-10-Azo-11-Glu into nanotubes demonstrated that chiral symmetry breaking effected by the azo group was also relevant to the chiral organization of the hiphiles. The chiral order of aggregate morphologies was additionally affected by the temperature and solvent composition of assembly in a manner correlated to the mechanism driving assembly i.e., D,L-10-Azo-11-Glu was sensitive to the temperature of assembly but less so to solvent composition, while L-14-Azo-11-Glu was sensitive to solvent composition and not to temperature. FTIR and UV-vis spectroscopic investigations into the organization of the head and azo groups, in chiral and achiral structures, illustrated that a balance of the influences of the hydrophilic and hydrophobic components on self-assembly was required for the optimization of the chiral organization of the self-assembled structures.
Anti-skin glycation and collagen level stimulation of brown seaweed extracts and their compositional characteristics
Publisher: Elsevier BV
Date: 09-2023
Growth and Survival of Early Juveniles of the Marine Sponge Hymeniacidon perlevis (Demospongiae) Under Controlled Conditions
Publisher: Springer Science and Business Media LLC
Date: 17-02-2009
DOI: 10.1007/S10126-009-9180-7
Abstract: To resolve "the supply problem" in sponge-derived drug development and other biotechnological applications, current research is exploring the possibility of obtaining an alternative sustainable supply of sponge biomass through intensive aquaculture of sponges utilizing artificial seed rearing. This study aimed to investigate the technology of early juvenile sponge cultivation under controlled conditions. The effects of food, temperature, water flow, and light on the growth and survival of early juveniles of the marine sponge Hymeniacidon perlevis were examined. The concentrations of four types of food elements [microalgae (Isochrysis galbana), photosynthetic bacteria (Rhodopseudomonas), Fe(3+) (FeCl(3)), and Si (Na(2)SiO(3))] were investigated for early H. perlevis juvenile growth. Interestingly, temperature changes have striking effects on juvenile growth. Juvenile sponges grow faster when they are shifted to higher temperatures (18 degrees C to 23 degrees C) than when they are shifted to lower temperatures (18 degrees C to 4 degrees C to 23 degrees C) or kept at a constant temperature (18 degrees C). Periodic water flow and light cycles favor early juvenile sponge growth. Light was found to be a key factor in the color loss of early H. perlevis juveniles. Overall, size (area) increased as much as 29 times for H. perlevis juveniles under the tested controlled conditions.
Release of encapsulated bioactives influenced by alginate viscosity under in-vitro gastrointestinal model
Publisher: Elsevier BV
Date: 02-2021
Bioactive sesquiterpene quinols and quinones from the marine sponge Dysidea avara
Publisher: Royal Society of Chemistry (RSC)
Date: 2015
DOI: 10.1039/C5RA18876H
Abstract: Reports the isolation, structure determination, and cytotoxic and NF-κB inhibitory activities of eight sesquiterpene quinols and quinones from Dysidea avara .
13th International Biotechnology Symposium and Exhibition: Biotechnology for the Sustainability of Human Society
Publisher: Springer Science and Business Media LLC
Date: 23-05-2009
Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis
Publisher: Elsevier BV
Date: 07-2004
Impact of extraction processes on prebiotic potential of the brown seaweed Ecklonia radiata by in vitro human gut bacteria fermentation
Publisher: Elsevier BV
Date: 06-2016
High-Shear-Imparted Tunable Fluorescence in Polyethylenimines
Publisher: No publisher found
Date: 2018
Quantitative analysis of hinokiol from cell suspension cultures of <I>Cephalotaxus fortunei</I>
Publisher: China Science Publishing & Media Ltd.
Date: 17-08-2011
DOI: 10.3724/SP.J.1123.2011.00567
Abstract: A high performance liquid chromatography (HPLC) method was developed for the separation of secondary metabolites and quantitative analysis of hinokiol from cell suspension cultures of Cephalotaxus fortunei. The s les were prepared by extraction using methanol followed by partitioning between ammonium hydroxide and chloroform. The HPLC separation was achieved on an Apollo C18 column (250 mm x 4.6 mm, 5 microm) with gradient elution using methanol and water at 1 mL/min and 30 degrees C. The detection was carried out at 290 nm. A good linear correlation between the hinokiol peak area and mass concentration was observed over the mass concentration range of 0.012 5 - 0.2 g/L. The proposed method was applied to the determination of hinokiol in the actual s les with recoveries of 87.2% - 94.7% and with the relative standard deviations of 0.9% - 4.2%. This method is reliable and reproducible and is suitable for the analysis of hinokiol in plant cell cultures.
Adsorption of doxorubicin hydrochloride on glutaric anhydride functionalized Fe3O4@SiO2 magnetic nanoparticles
Publisher: Elsevier BV
Date: 05-2019
DOI: 10.1016/J.MSEC.2018.12.145
Abstract: Since Fe
High temporal variability in bacterial community, silicatein and hsp70 expression during the annual life cycle of Hymeniacidon sinapium (Demospongiae) in China's Yellow Sea
Publisher: Elsevier BV
Date: 08-2012
Structural Elucidation of Novel Saponins in the Sea Cucumber Holothuria lessoni
Publisher: MDPI AG
Date: 08-08-2014
DOI: 10.3390/MD12084439
Antifungal bromopyrrole alkaloids from the South China Sea sponge Agelas sp.
Publisher: Elsevier BV
Date: 06-2016
Role of Dietary Nutrients in the Modulation of Gut Microbiota: A Narrative Review
Publisher: MDPI AG
Date: 31-01-2020
DOI: 10.3390/NU12020381
Abstract: Understanding how dietary nutrients modulate the gut microbiome is of great interest for the development of food products and eating patterns for combatting the global burden of non-communicable diseases. In this narrative review we assess scientific studies published from 2005 to 2019 that evaluated the effect of micro- and macro-nutrients on the composition of the gut microbiome using in vitro and in vivo models, and human clinical trials. The clinical evidence for micronutrients is less clear and generally lacking. However, preclinical evidence suggests that red wine- and tea-derived polyphenols and vitamin D can modulate potentially beneficial bacteria. Current research shows consistent clinical evidence that dietary fibers, including arabinoxylans, galacto-oligosaccharides, inulin, and oligofructose, promote a range of beneficial bacteria and suppress potentially detrimental species. The preclinical evidence suggests that both the quantity and type of fat modulate both beneficial and potentially detrimental microbes, as well as the Firmicutes/Bacteroides ratio in the gut. Clinical and preclinical studies suggest that the type and amount of proteins in the diet has substantial and differential effects on the gut microbiota. Further clinical investigation of the effect of micronutrients and macronutrients on the microbiome and metabolome is warranted, along with understanding how this influences host health.
Sequential extraction and characterization of fucoidans and alginates from Ecklonia radiata, Macrocystis pyrifera, Durvillaea potatorum, and Seirococcus axillaris
Publisher: Springer Science and Business Media LLC
Date: 10-11-2017
Photo-biological hydrogen production system by green microalgae: Theoretical analysis for constraints and prospects
Publisher: Elsevier BV
Date: 11-2010
Discovery of Novel Saponins from the Viscera of the Sea Cucumber Holothuria lessoni
Publisher: MDPI AG
Date: 09-05-2014
DOI: 10.3390/MD12052633
Two structural types of dithiocarbamato-chlorido complexes of mercury(II): Preparation, supramolecular self-assembly, solid-state 13C and 15N NMR characterisation and thermal behaviour of pseudo-polym
Publisher: Elsevier BV
Date: 04-2022
Ligand Mediated, Spatially Confined Carbonization of Biomass Forming High-Performance Colloidal Carbon Dots
Publisher: American Chemical Society (ACS)
Date: 14-07-2023
Magnetic Fe3O4@ZIF-8 nanoparticles as a drug release vehicle
Publisher: Elsevier BV
Date: 03-2023
Special section on Biotechnology for the Sustainability of Human Society
Publisher: Elsevier BV
Date: 11-2009
Critical evaluation of process parameters for direct biodiesel production from diverse feedstock
Publisher: Elsevier BV
Date: 05-2020
Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures
Publisher: Elsevier BV
Date: 03-2002
Phylogenetic diversity of Gram-positive bacteria cultured from Antarctic deep-sea sponges
Publisher: Springer Science and Business Media LLC
Date: 17-04-2011
Effects of nutrient deprivation on biochemical compositions and photo-hydrogen production of Tetraselmis subcordiformis
Publisher: Elsevier BV
Date: 05-2011
Continuous flow biodiesel production from wet microalgae using a hybrid thin film microfluidic platform
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C8CC07610C
Abstract: A novel continuous flow turbo-thin film device (T 2 FD) is effective in producing biodiesel in high yield from wet microalgae at room temperature.
Neuroprotective Activities of Marine Natural Products from Marine Sponges
Publisher: Bentham Science Publishers Ltd.
Date: 04-02-2016
DOI: 10.2174/0929867323666151127201249
Abstract: This review covers the compounds isolated from marine sponges with neuroprotective activities during the period between 1999 and 2014 based on their chemical structures, collections sites, sponge taxonomy and neuroprotective effects. These compounds were isolated from marine sponges collected from 18 countries, most of them in Indonesia, followed by Japan. A total of 90 compounds were reported to exhibit a range of neuroprotective efficacy. These compounds were shown to inhibit β-site amyloid precursor protein (APP) cleaving enzyme 1 (BACE1), modulate the synthesis or activity of some neurotransmitters such as acetylcholinesterase and glutamate, enhancement of serotonin, reducing oxidative stress, inhibition of kinases and proteases, and enhancement of neurite growth. None of them have yet progressed into any marine pharmaceutical development pipeline, therefore sustained researches will be required to enhance the potential of utilizing these compounds in the future for prevention and therapeutic treatment of neurodegenerative diseases.
Advances on marine-derived natural radioprotection compounds: historic development and future perspective
Publisher: Springer Science and Business Media LLC
Date: 12-05-2021
DOI: 10.1007/S42995-021-00095-X
Abstract: Natural extracts and compounds from marine resources have gained intensive scientific and industry attention for radioprotective activities in the past ten years. However, the marine-derived radioprotectants have been studied against UV-rays, gamma (γ)-rays and X-rays for more than 30 years. This review aims to identify key marine-derived extracts/compounds and their modes of action studied for radioprotective activities from 1986 to 2019. A comprehensive survey was conducted to establish the trend in terms of the publications each year and the countries of origin. A total of 40 extracts and 34 natural compounds showing radioprotective activities against UV-rays, gamma (γ)-rays and X-rays were identified from a range of marine plants and animals. These extracts and compounds are broadly categorized into polysaccharides, phlorotannins, carotenoids and mycosporine-like amino acids (MAAs). Macroalgae and microalgae were found to be the dominant sources of polysaccharides, phlorotannins and carotenoids. MAAs were mainly identified in algae, sponges, sea cucumber and corals that showed significant UV-absorbing activities. A number of radioprotective mechanisms were shown by these compounds, predominantly free radicals scavenging, inhibition of apoptosis, UV-ray absorption and DNA damage-repair signaling pathways. While these bio-discoveries warrant further investigation and development of radioprotective therapeutics, however, the lack of clinical studies is a major obstacle to be tackled in the future.
Impact of Ecklonia radiata extracts on the neuroprotective activities against amyloid beta (Aβ1-42) toxicity and aggregation
Publisher: Elsevier BV
Date: 05-2020
Microwave-Intensified Enzymatic Deproteinization of Australian Rock Lobster Shells (Jasus edwardsii) for the Efficient Recovery of Protein Hydrolysate as Food Functional Nutrients
Publisher: Springer Science and Business Media LLC
Date: 14-12-2015
In vitro studies of the neuroprotective activities of astaxanthin and fucoxanthin against amyloid beta (Aβ 1-42 ) toxicity and aggregation
Publisher: Elsevier BV
Date: 03-2019
DOI: 10.1016/J.NEUINT.2019.01.010
Abstract: Amyloid beta (Aβ) can aggregate and form plaques, which are considered as one of the major hallmarks of Alzheimer's disease. This study aims to directly compare the neuroprotective activities in vitro of two marine-derived carotenoids astaxanthin and fucoxanthin that have shown a spectrum of biological activities, including neuroprotection. The in vitro neuroprotective activities were investigated against Aβ
Development of an eco-friendly agar extraction technique from the red seaweed Gracilaria lemaneiformis
Publisher: Elsevier BV
Date: 05-2008
DOI: 10.1016/J.BIORTECH.2007.07.002
Abstract: The red seaweed, Gracilaria lemaneiformis growing as an aquaculture bioremediator along the coasts of Liaodong Peninsula, China, was investigated for the agar production. An eco-friendly method called agar photobleaching extraction process was developed for the benefit of workers' health and safety of the environment. The native agar (NA), alkali-modified agar (AA), chemical-bleached agar (CA) and photobleached agar (PA), which were extracted using different processes, were evaluated for their physical and chemical properties. The PA showed most desirable performances in terms of gel strength, gelling temperature, sulfate content and 3,6-anhydro-l-galactose content. Among the different processed agars, PA gel strength was 1913 g/cm2, the highest among the different processed agars, which increased 8.6% on the basis of the AA. Further we applied this new technique to extract agars from Gracilaria asiatica, and similar results were obtained with that of G. lemaneiformis. This indicates that the agar photobleaching extraction process is a feasible method for Gracilaria species and has a potential application. During the whole agar photobleaching extraction process the pigment content of G. lemaneiformis declined gradually and the TOC concentration in photobleaching solution increased along with the increase in the irradiation time. The mechanism of agar photobleaching could be elucidated by the photolysis theory.
Hot water pretreatment-induced significant metabolite changes in the sea cucumber Apostichopus japonicus
Publisher: Elsevier BV
Date: 06-2020
DOI: 10.1016/J.FOODCHEM.2020.126211
Abstract: Hot water pretreatment of sea cucumbers potentially changes nutritional benefits. This study aimed to quantify hot water pretreatment-induced changes in metabolite profiles of sea cucumber body walls. ICP-OES, GC-MS, and LC-MS analyses of untreated- (UT-BW), hot water-treated body walls (HW-BW) of Apostichopus japonicus, and the hot water extract (HW-E) determined significant losses of minerals (25-50% w/w), protein (~11% w/w), carbohydrate (33% w/w), saponins (~41% w/w), and spermidine (100%), a potential antipsychotic from hot water-treated s les. Multivariate comparisons of HW-BW with UT-BW and HW-BW with HW-E showed increases in amino acids and fatty acids, suggesting hot water-induced degradation or transformation or easier extraction of protein, lipid or other components. Presence of 80 to 88.5% of compounds in the HW-E and lower DHA, EPA and glycerophospholipids levels in HW-BW suggested extraction of these metabolites. These data indicate that novel processing technologies are required to preserve the full nutritional benefits of sea cucumbers.
A two-stage process with temperature-shift for enhanced anthocyanin production in strawberry cell suspension cultures
Publisher: Springer Science and Business Media LLC
Date: 08-1999
DOI: 10.1007/BF02873962
Purification, molecular cloning, and characterization of glutathione S-transferases (GSTs) from pigmented Vitis vinifera L. cell suspension cultures as putative anthocyanin transport proteins
Publisher: Oxford University Press (OUP)
Date: 10-2008
DOI: 10.1093/JXB/ERN217
Bioremediation of bacteria pollution using the marine sponge Hymeniacidon perlevis in the intensive mariculture water system of turbot Scophthalmus maximus
Publisher: Wiley
Date: 10-2009
DOI: 10.1002/BIT.22522
Abstract: Sessile filter-feeding marine sponges (Porifera) have been reported to possess high efficiency in removing bacteria pollution from natural or aquaculture seawater. However, no investigation has been carried out thus far in a true mariculture farm water system. Therefore this study sought to investigate the ability of the marine sponge Hymeniacidon perlevis to bioremediate the bacteria pollution in the intensive aquaculture water system of turbot Scophthalmus maximus. Sponge specimens were hung in fish culture effluent at different temperature to investigate the optimal temperature condition for bacteria removal by H. perlevis. Turbots S. maximus were co-cultured with sponge H. perlevis in 1.5 m(3) of water system at 15-18 degrees C for 6 weeks to control the growth of bacteria. It was found that H. perlevis was able to remove pathogenic bacteria efficiently at 10-20 degrees C, with a maximal removal of 71.4-78.8% of fecal coliform, 73.9-98.7% of pathogenic vibrio, and 75.0-83.7% of total culturable bacteria from fish-culture effluent at 15 degrees C H. perlevis continuously showed good bioremediation of bacteria pollution in the S. maximus culture water system, achieving removal of 60.0-90.2% of fecal coliform, 37.6-81.6% of pathogenic vibrio, and 45.1-83.9% of total culturable bacteria. The results demonstrate that H. perlevis is an effective bioremediator of bacteria pollution in the turbot S. maximus culture farm water system.
Lobster processing by-products as valuable bioresource of marine functional ingredients, nutraceuticals, and pharmaceuticals
Publisher: Springer Science and Business Media LLC
Date: 22-06-2017
Marine Sponge Derived Natural Products between 2001 and 2010: Trends and Opportunities for Discovery of Bioactives
Publisher: MDPI AG
Date: 19-08-2014
DOI: 10.3390/MD12084539
Seaweed and seaweed-derived metabolites as prebiotics
Publisher: Elsevier
Date: 2020
Anthocyanins—More Than Nature's Colours
Publisher: Hindawi Limited
Date: 2004
Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)
Publisher: Springer Science and Business Media LLC
Date: 06-12-2006
DOI: 10.1007/S00441-006-0342-X
Abstract: Marine sponges (Porifera) are the best source of marine bioactive metabolites for drug discovery and development, although the sustainable production of most sponge-derived metabolites remains a difficult task. In vitro cultivation of sponge cells in bioreactors has been proposed as a promising technology. However, no continuous cell line has as yet been developed. Archaeocytes are considered to be toti/multipotent stem cells in sponges and, when purified, may allow the development of continuous sponge cell lines. As a prerequisite, we have developed a novel four-step protocol for the purification of archaeocytes from a marine sponge, Hymeniacidon perleve: (1) differential centrifugation to separate large sponge cells including archaeocytes (2) selective agglomeration in low-Ca(2+)/Mg(2+) artificial seawater in which living archaeocytes form small loose aggregates with some pinacocytes and collencytes (3) differential adherence to remove anchorage-dependent pinacocytes, collencytes and other mesohyl cells (4) Ficoll-Vrografin density gradient centrifugation to purify archaeocytes. The final purity of archaeocytes is greater than 80%. The proliferation potential of the archaeocytes has been demonstrated by high levels of BrdU incorporation, PCNA expression and telomerase activity. In 4-day primary cultures, the purified archaeocytes show a 2.5-fold increase in total cell number. This study opens an important avenue towards developing sponge cell cultures for the commercial exploitation of sponge-derived drugs.
Vortex Fluidic Mediated Synthesis of Macroporous Bovine Serum Albumin-Based Microspheres
Publisher: American Chemical Society (ACS)
Date: 20-07-2018
Abstract: Macroporous bovine serum albumin (BSA) nanoparticles with controllable diameter were readily fabricated in a rapidly rotating angled glass tube in a vortex fluidic device (VFD). Systematically varying the rotational speed and the ratio of BSA, ethanol, and glutaraldehyde led to conditions for generating ca. 600 nm diameter macroporous particles that have intrinsic fluorescence emission at 520 nm when excited at 490 nm. The presence of the macropores increased the absorption efficiency of rhodamine B with potential applications for drug delivery purpose, compared with BSA nanoparticles having surfaces devoid of pores. Further control over the size of BSA nanoparticles occurred in the presence of C-phycocyanin protein during the VFD processing, along with control of their shape, from spheres to pockets, as established in exploring the parameter space of the microfluidic device.
Anthocyanic vacuolar inclusions (AVIs) selectively bind acylated anthocyanins in Vitis vinifera L. (grapevine) suspension culture.
Publisher: Springer Science and Business Media LLC
Date: 2003
Abstract: Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolated from the same callus accumulating anthocyanin in the dark, yet varying in their anthocyanin profiles and accumulation. An intermediate-pigmented line (FU-1) with a 1.3:1 ratio of acylated:non-acylated anthocyanins, a colour value of 0.84 units and cyanidin and peonidin as the dominant species was compared with a high-pigmented line (FU-2) with a 1.2:1 ratio of acylated:non-acylated anthocyanins, a colour value of 3.72 units and malvidin predominating. The profile of AVI-bound anthocyanins showed an increase in acylated anthocyanins in both lines of approx. 28-29%, with no apparent preference for anthocyanin species. This resulted in a ratio of acylated:non-acylated anthocyanins of 6.2:1 for FU-1 and 4.9:1 for FU-2. The reasons for the selectivity of the AVIs for acylated (specifically p-coumaroylated) species compared with the whole cell profile are discussed.
Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field
Publisher: Springer Science and Business Media LLC
Date: 26-06-2007
DOI: 10.1007/S00441-007-0443-1
Abstract: To characterize the formation of silica spicules, the dynamics of spiculogenesis of an intertidal marine sponge Hymeniacidon perlevis (Montagu 1818) (Porifera: Demospongiae) were investigated by measuring the gene expression of silicatein (the enzyme responsible for spicule silicification) and the dimensional changes of spicules during the developmental process of in idual sponges and in cell cultures of primmorphs of archaeocyte-dominant cell populations. The different developmental stages of spicules were documented by time-lapse microscopy and observed by transmission electron microscopy during a 1-month culture period. During its annual life cycle, H. perlevis has four different developmental stages: dormancy, resuscitation, bloom, and decline. Field-grown in idual sponge s les at different stages were collected over 7 months (March to September 2005). The dimensions of the silica spicules from these s les were microscopically measured and statistically analyzed. This analysis and the material properties of the spicules allowed them to be classified into four groups representing the different developmental stages of spiculogenesis. Silicatein expression in the bloom stage was more than 100 times higher than that in the other stages and was correlated with the spicule developmental stage. The trend of spicule formation in field-grown sponges was consistent with the trend in cell culture. A new parameter, the maturation degree (MD) of spicules (defined as the ratio of actual to theoretical silica deposition of mature spicules), was introduced to quantify spicule development. Silica spiculogenesis during H. perlevis development was delineated by comparing MD and silicatein expression.
Anti-skin cancer properties of phenolic-rich extract from the pericarp of mangosteen (Garcinia mangostana Linn.)
Publisher: Elsevier BV
Date: 09-2012
DOI: 10.1016/J.FCT.2012.06.003
Abstract: Skin cancers are often resistant to conventional chemotherapy. This study examined the anti-skin cancer properties of crude ethanol extract of mangosteen pericarp (MPEE) on human squamous cell carcinoma A-431 and melanoma SK-MEL-28 lines. Significant dose-dependent reduction in% viability was observed for these cell lines, with less effect on human normal skin fibroblast CCD-1064Sk and keratinocyte HaCaT cell lines. Cell distribution in G(1) phase (93%) significantly increased after 10 μg/ml of MPEE versus untreated SK-MEL-28 cells (78%), which was associated with enhanced p21(WAF1) mRNA levels. In A-431 cells, 10 μg/ml MPEE significantly increased the sub G(1) peak (15%) with concomitant decrease in G(1) phase over untreated cells (2%). In A-431 cells, 10 μg/ml MPEE induced an 18% increase in early apoptosis versus untreated cells (2%). This was via caspase activation (15-, 3- and 4-fold increased caspse-3/7, 8, and 9 activities), and disruption of mitochondrial pathways (6-fold decreased mitochondrial membrane potential versus untreated cells). Real-time PCR revealed increased Bax/Bcl-2 ratio and cytochrome c release, and decreased Akt1. Apoptosis was significantly increased after MPEE treatment of SK-MEL-28 cells. Hence, MPEE showed strong anti-skin cancer effect on these two skin cancer cell lines, with potential as an anti-skin cancer agent.
Significant increase in the secretion of extracellular vesicles and antibiotics resistance from methicillin-resistant Staphylococcus aureus induced by ampicillin stress
Publisher: Springer Science and Business Media LLC
Date: 03-12-2020
DOI: 10.1038/S41598-020-78121-8
Abstract: Extracellular vesicles (EVs) containing specific cargo molecules from the cell of origin are naturally secreted from bacteria. EVs play significant roles in protecting the bacterium, which can contribute to their survival in the presence of antibiotics. Herein, we isolated EVs from methicillin-resistant Staphylococcus aureus (MRSA) in an environment with or without stressor by adding icillin at a lower concentration than the minimum inhibitory concentration (MIC). We investigated whether EVs from MRSA under stress condition or normal condition could defend susceptible bacteria in the presence of several β-lactam antibiotics, and directly degrade the antibiotics. A comparative proteomic approach was carried out in both types of EVs to investigate β-lactam resistant determinants. The secretion of EVs from MRSA under antibiotic stressed conditions was increased by 22.4-fold compared with that of EVs without stress. Proteins related to the degradation of β-lactam antibiotics were abundant in EVs released from the stressed condition. Taken together, the present data reveal that EVs from MRSA play a crucial role in the survival of β-lactam susceptible bacteria by acting as the first line of defense against β-lactam antibiotics, and antibiotic stress leads to release EVs with high defense activity.
Characterization of silica spicule formation during the resuscitation and in vitro cell culture of Hymeniacidon perleve
Publisher: Elsevier BV
Date: 07-2006
Highly efficient recovery of nutritional proteins from Australian Rock Lobster heads (Jasus edwardsii) by integrating ultrasonic extraction and chitosan co-precipitation
Publisher: Elsevier BV
Date: 03-2020
International Workshop on Anthocyanins 2002
Publisher: Elsevier BV
Date: 06-2003
Purification and characterization of 2-haloacid dehalogenase from marine bacterium Paracoccus sp. DEH99, isolated from marine sponge Hymeniacidon perlevis
Publisher: Springer Science and Business Media LLC
Date: 26-01-2014
Selective feeding by sponges on pathogenic microbes: a reassessment of potential for abatement of microbial pollution
Publisher: Inter-Research Science Center
Date: 22-03-2010
DOI: 10.3354/MEPS08411
A comparative study on the phylogenetic diversity of culturable actinobacteria isolated from five marine sponge species
Publisher: Springer Science and Business Media LLC
Date: 24-08-2007
DOI: 10.1007/S10482-007-9196-9
Abstract: A cultivation-based approach was employed to compare the culturable actinobacterial ersity associated with five marine sponge species (Craniella australiensis, Halichondria rugosa, Reniochalina sp., Sponge sp., and Stelletta tenuis). The phylogenetic affiliation of the actinobacterial isolates was assessed by 16S rDNA-RFLP analysis. A total of 181 actinobacterial strains were isolated using five different culture media (denoted as M1-M5). The type of medium exhibited significant effects on the number of actinobacteria recovered, with the highest number of isolates on M3 (63 isolates) and the lowest on M1 (12 isolates). The genera isolated were also different, with the recovery of three genera on M2 and M3, and only a single genus on M1. The number of actinobacteria isolated from the five sponge species was significantly different, with a count of 83, 36, 30, 17, and 15 isolates from S. tenuis, H. rugosa, Sponge sp., Reniochalina sp., and C. australiensis, respectively. M3 was the best isolation medium for recovery of actinobacteria from S. tenuis, H. rugosa, and Sponge sp., while no specific medium preference was observed for the recovery of actinobacteria from Reniochalina sp., and C. australiensis. The RFLP fingerprinting of 16S rDNA genes digested with HhaI revealed six different patterns, in which 16 representative 16S rDNAs were fully sequenced. Phylogenetic analysis indicated that 12 strains belong to the group Streptomyces, three strains belong to Pseudonocardia, and one strain belongs to Nocardia. Two strains C14 (from C. australiensis) and N13 (from Sponge sp.) have only 96.26% and 96.27% similarity to earlier published sequences, and are therefore potential candidates for new species. The highest ersity of three actinobacteria genera was obtained from Sponge sp., though the number of isolates was low. Two genera of actinobacteria, Streptomyces, and Pseudonocardia, were isolated from both S. tenuis and C. australiensis. Only the genus of Streptomyces was isolated from H. rugosa and Reniochalina sp. Sponge species have been demonstrated here to vary as sources of culturable actinobacterial ersity, and the methods for s ling such ersity presented may be useful for improved s ling of such ersity.
Improved antioxidant activities of brown seaweed Ecklonia radiata extracts prepared by microwave-assisted enzymatic extraction
Publisher: Springer Science and Business Media LLC
Date: 08-12-2015
Study on bioactivity of extracts from marine sponges in Chinese Sea
Publisher: Elsevier BV
Date: 2004
Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis
Publisher: Springer Science and Business Media LLC
Date: 03-11-2010
DOI: 10.1007/S10529-010-0454-Y
Abstract: A novel, simple and highly efficient process for purifying vanadium bromoperoxidase from Corallina officinalis is reported. The key innovation is adding 0.5 mM sodium orthovanadate to the crude cell extract followed by heating at 70°C for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. Combining the heat treatment with ammonium sulfate precipitation and DEAE-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. Finally, a specific activity of 310 U/mg, a 27-fold purification of the crude enzyme solution was produced.
Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers.
Publisher: Wiley
Date: 2003
DOI: 10.1021/BP034106K
Biotechnology in China I
Publisher: Springer Berlin Heidelberg
Date: 2009
Neuroprotective activities of natural products from marine macroalgae during 1999–2015
Publisher: Springer Science and Business Media LLC
Date: 05-08-2016
Marine biorefinery: an environmentally sustainable solution to turn marine biomass and processing wastes into value-added products and profits
Publisher: Portland Press Ltd.
Date: 04-04-2022
DOI: 10.1042/BIO_2022_105
Laser irradiated vortex fluidic mediated synthesis of luminescent carbon nanodots under continuous flow
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C7RE00197E
Abstract: High shear vortex fluidics coupled with NIR affords luminescent carbon dots as a scalable process.
Comparison of Spiculogenesis in in Vitro ADCP-primmorph and Explants Culture of Marine Sponge Hymeniacidon perleve with 3-TMOSPU Supplementation
Publisher: Wiley
Date: 05-09-2008
DOI: 10.1021/BP0603659
Abstract: This study aims to test the feasibility of introducing functional chemical groups into biogenic silica spicules by examining the effect of supplementing a silican coupler [3-(trimethoxysilyl)propyl]urea (3-TMOSPU) as silica source in the cultures of archaeocytes-dominant-cell-population (ADCP) primmorphs and explants of the marine sponge Hymeniacidon perleve. Analysis by Fourier Transform Infrared Spectroscopy (FT-IR) confirmed that the organic group in 3-TMOSPU was introduced into silica spicules. By comparing ADCP-primmorph cultures when supplemented with Na2SiO3, 3-TMOSPU supplementation showed no notable effect on the primmorphs development and cell locomotion behaviors. A decline in silicatein expression quantified by real-time RT-PCR was, however, observed during spiculogenesis. The decline was slower for the 3-TMOSPU group whereas significantly fewer spicules were formed. When sponge papillae explants were cultured, 3-TMOSPU supplementation had no negative effect on sponge growth but inhibited the growth biofouling of the diatom Nitzschia closterium. By monitoring the detectable Si concentration, it seemed that 3-TMOSPU was converted by the sponge and its conversion was related to spiculogenesis. Analysis of spicule dimensional changes indicated that the inhibition of spiculogenesis by 3-TMOSPU supplementation was less in ADCP-primmorphs culture due to lower 3-TMOSPU/detectable Si ratio in the media.
Sub-micron moulding topological mass transport regimes in angled vortex fluidic flow
Publisher: Royal Society of Chemistry (RSC)
Date: 2021
DOI: 10.1039/D1NA00195G
Abstract: A rapidly rotating tube in the vortex fluidic device imparts submicron topological mass transport regimes, as moulded through crystallisation, polymerisation, and ‘molecular drilling’.
Formulation of a Basal Medium for Primary Cell Culture of the Marine Sponge Hymeniacidon perleve
Publisher: Wiley
Date: 05-09-2008
DOI: 10.1021/BP050029C
Abstract: Marine sponge cell culture is a potential route for the sustainable production of sponge-derived bioproducts. Development of a basal culture medium is a prerequisite for the attachment, spreading, and growth of sponge cells in vitro. With the limited knowledge available on nutrient requirements for sponge cells, a series of statistical experimental designs has been employed to screen and optimize the critical nutrient components including inorganic salts (ferric ion, zinc ion, silicate, and NaCl), amino acids (glycine, glutamine, and aspartic acid), sugars (glucose, sorbitol, and sodium pyruvate), vitamin C, and mammalian cell medium (DMEM and RPMI 1640) using MTT assay in 96-well plates. The marine sponge Hymeniacidon perleve was used as a model system. Plackett-Burman design was used for the initial screening, which identified the significant factors of ferric ion, NaCl, and vitamin C. These three factors were selected for further optimization by Uniform Design and Response Surface Methodology (RSM), respectively. A basal medium was finally established, which supported an over 100% increase in viability of sponge cells.
Fresh living Arthrospira as dietary supplements
Publisher: Elsevier BV
Date: 06-2019
Characterizing the Heterogeneity of an Immobilized Cell Gel Matrix
Publisher: Wiley
Date: 10-12-2002
DOI: 10.1002/1618-2863(20021210)2:12<409::AID-ELSC409>3.0.CO;2-T
Enzyme-assisted extraction of carbohydrates from the brown alga Ecklonia radiata: Effect of enzyme type, pH and buffer on sugar yield and molecular weight profiles
Publisher: Elsevier BV
Date: 10-2016
Combining the Anticancer and Immunomodulatory Effects of Astragalus and Shiitake as an Integrated Therapeutic Approach
Publisher: MDPI AG
Date: 27-07-2021
DOI: 10.3390/NU13082564
Abstract: Astragalus root (Huang Qi) and Shiitake mushrooms (Lentinus edodes) are both considered medicinal foods and are frequently used in traditional Chinese medicine due to their anticancer and immunomodulating properties. Here, the scientific literatures describing evidence for the anticancer and immunogenic properties of Shiitake and Astragalus were reviewed. Based on our experimental data, the potential to develop medicinal food with combined bioactivities was assessed using Shiitake mushrooms grown over Astragalus beds in a proprietary manufacturing process, as a novel cancer prevention approach. Notably, our data suggest that this new manufacturing process can result in transfer and increased bioavailability of Astragalus polysaccharides with therapeutic potential into edible Shiitake. Further research efforts are required to validate the therapeutic potential of this new Hengshan Astragalus Shiitake medicinal food.
Gut health benefits of brown seaweed Ecklonia radiata and its polysaccharides demonstrated in vivo in a rat model
Publisher: Elsevier BV
Date: 10-2017
Preparation of a microalgal photoanode for hydrogen production by photo-bioelectrochemical water-splitting
Publisher: Elsevier BV
Date: 09-2013
Improved hydrogen photoproduction regulated by carbonylcyanide m-chlorophenylhrazone from marine green alga Platymonas subcordiformis grown in CO2-supplemented air bubble column bioreactor
Publisher: Springer Science and Business Media LLC
Date: 15-01-2008
DOI: 10.1007/S10529-008-9637-1
Abstract: To develop an integrated process of CO(2)-fixation and H(2) photoproduction by marine green microalga Platymonas subcordiformis, the impact of algal cells grown in CO(2)-supplemented air bubble column bioreactor was investigated on H(2) photoproduction regulated by carbonylcyanide m-chlorophenylhrazone. Highest cell growth (3.85 x 10(6) cells ml(-1)), starch content (0.25 +/- 0.08 mg per 10(6)cells) and hydrogen production (50 +/- 3 ml l(-1)) were achieved at 3% CO(2)-supplemented culture, which are respectively 1.4, 2.1, 1.5-fold of the air-supplemented culture. Improved H(2) production correlated well with the increase in starch accumulation. In this process, the algal cells have been recycled for stable H(2) production of 40-50 ml l(-1) over five cycles.
Chiral Self-Assembly of Designed Amphiphiles: Optimization for Nanotube Formation
Publisher: American Chemical Society (ACS)
Date: 26-09-2012
DOI: 10.1021/LA3030606
Abstract: Four hiphiles with L-aspartic acid headgroups (Asp) and a diphenyldiazenyl group (Azo) contained within the hydrophobic tails were designed and synthesized for self-assembly into helically based nanotubes. The hiphiles of the form R'-{4-[(4-alkylphenyl)diazenyl]phenoxy}alkanoyl-L-aspartic acid (where R' is 10 or 11) varied only in alkyl chain lengths either side of the azo group, having 4, 7, or 10 carbon distal chains and 10 or 11 carbon proximal chains (R-Azo-R'-Asp, where R denotes the number of carbons in the distal chain and R' denotes the number of carbons in the proximal chain). Despite the molecular similarities, distinct differences were identified in the chiral order of the structures self-assembled from hot methanolic aqueous solutions using microscopy and spectroscopic analyses. This was reflected in dominant thermodynamic aggregate morphologies that ranged from amorphous material for 10-Azo-10-Asp, through twisted ribbons (196 ± 49 nm pitch) for 7-Azo-11-Asp, to the desired helically based nanotubes for 4- and 7-Azo-10-Asp (81 ± 11 and 76 ± 6 nm diameters, respectively). Another key variable in the self-assembly of the hiphiles was the use of a second method to precipitate aggregates from solution at room temperature. This method enabled the isolation of thermodynamically unstable and key transitional structures. Helical ribbons were precursor structures to the nanotubes formed from 4- and 7-Azo-10-Asp as well as the wide, flattened nanotube structures (587 ± 85 nm width) found for 4-Azo-10-Asp. Overall, the results highlighted the interplay of influence of the headgroup and the hydrophobic tail on self-assembly, providing a basis for future rational design of self-assembling hiphiles.
Production of anthocyanins by plant cell cultures
Publisher: Springer Science and Business Media LLC
Date: 12-1999
DOI: 10.1007/BF02933747
Culture-independent nested PCR method reveals high diversity of actinobacteria associated with the marine sponges Hymeniacidon perleve and Sponge sp.
Publisher: Springer Science and Business Media LLC
Date: 08-2008
DOI: 10.1007/S10482-008-9270-Y
Abstract: A culture-independent nested polymerase chain reaction (PCR) technique was used to investigate the ersity of actinobacteria communities associated with the sponges Hymeniacidon perleve and Sponge sp. The phylogenetic affiliation of sponge-derived actinobacteria was then assessed by 16S rRNA sequencing of cloned DNA fragments. A total of 196 positive clones were screened by restriction fragment length polymorphism (RFLP) analysis 48 unique operational taxonomic units (OTUs) were selected for sequencing. Rarefaction analysis indicated that the clone libraries represented 93% and 94% of the total estimated ersity for the two species, respectively. Phylogenetic analysis of sequence data revealed representatives of various phylogenetic isions, which were related to the following ten actinobacterial genera: Acidimicrobium, Corynebacterium, Propionibacterium, Actinomyces, Micrococcus, Microbacterium, Streptomyces, Mycobacterium, Cellulosimicrobium, Sporichthya, and unidentified actinobacterial clones. A sponge-specific, previously uncultured actinobacteria community grouped within the subclass Acidimicrobidae was discovered from both H. perleve and Sponge sp. Sequences belonging to Acidimicrobium in the H. perleve and the Sponge sp. clone libraries represented 33% and 24% of the clones, respectively. In the Sponge sp. clone library Mycobacterium dominated, accounting for 70% of all clones. The presence of Acidimicrobium and mycobacteria within two sponges can lay the groundwork for attempts to culture these interesting bacteria for industrial applications.
Towards manipulation of post-biosynthetic events in secondary metabolism of plant cell cultures
Publisher: Elsevier BV
Date: 05-2002
Optimization and scale-up of a new photobleaching agar extraction process from Gracilaria lemaneiformis
Publisher: Springer Science and Business Media LLC
Date: 08-2008
Growth and lactic acid production in batch culture of Lactobacillus rhamnosus in a defined medium
Publisher: Springer Science and Business Media LLC
Date: 1999
Formation, purification and characterization of anthocyanic vacuolar inclusions in Vitis vinifera L. cell suspension cultures
Publisher: Elsevier BV
Date: 11-2010
Role of Carbonyl Cyanide m-Chlorophenylhydrazone in Enhancing Photobiological Hydrogen Production by Marine Green Alga Platymonas subcordiformis
Publisher: Wiley
Date: 07-04-2006
DOI: 10.1021/BP050289U
Abstract: We demonstrated that a significant volume of H(2) gas could be photobiologically produced by a marine green alga Platymonas subcordiformis when an uncoupler of photophosphorylation, carbonyl cyanide m-chlorophenylhydrazone (CCCP), was added after 32 h of anaerobic dark incubation, whereas a negligible volume of H(2) gas was produced without CCCP. The role of CCCP in enhancing photobiological H(2) production was delineated. CCCP as an ADRY agent (agent accelerating the deactivation reactions of water-splitting enzyme system Y) rapidly inhibited the photosystem II (PSII) activity of P. subcordiformis cells, resulting in a markedly decline in the coupled oxygen evolution. The mitochondrial oxidative respiration was only slightly inactivated by CCCP, which depleted O(2) in the light. As a result, anaerobiosis during the stage of photobiological H(2) evolution was established, preventing severe O(2) inactivation of the reversible hydrogenase in P. subcordiformis. The uncoupling effect of CCCP accelerates electron transfer from water due to a disruption of the proton motive force and release of DeltapH across the thylakoid membrane and thus enhances the accessibility of electron and H(+) to hydrogenase. The electrons for hydrogen photoevolution are mainly from the photolysis of water (90%). Upon the addition of CCCP, Chl a/b ratio increased, which implies a decrease in the light-harvesting PSII antennae or an increase in PSII/PSI ratio, possibly resulting in higher efficiency of utilization of light energy. The enhancement of H(2) evolution by the addition of CCCP is mostly due to the combination of the above three mechanisms. However, the disruption of the proton gradient across the thylakoid membrane may prevent a sustained photobiological H(2) evolution due to a shortfall of ATP generation essential for the maintenance and repair functions of the cells.
High‐Shear‐Imparted Tunable Fluorescence in Polyethylenimines
Publisher: Wiley
Date: 25-01-2018
Impact of subculture cycles and inoculum sizes on suspension cultures of Vitis vinifera
Publisher: Elsevier BV
Date: 11-2006
DOI: 10.1016/S1872-2075(06)60068-X
Abstract: The commercial application of plant cell cultures is often hindered by the instability of secondary metabolite biosynthesis, where the metabolite yield fluctuates and decline dramatically over subcultures. This study proposed that such instability is due to the fluctuations of culture variables. To validate this hypothesis, the effects of the fluctuations of two culture variables (subculture cycle and inoculum size) on the biomass, anthocyanin biosynthesig, intracellular carbon, nitrogen and phosphate during continuous 10 subculture cycles were investigated. The subculture cycle was fluctuated for 12h in a 7 day cycle (6.5, 7 and 7.5 d), and the inoculum size was fluctuated by 20% on basis of 2.00 g (1.60, 2.00 and 2.40 g). It was found that all the measured culture parameters fluctuated over the 10 subculture cycles. The fluctuation in terms of inoculum sizes had a greater effect on the stability of anthocyanin biosynthesis in suspension cultures of V. vinifera. Among all the subculture conditions investigated, 7d-subculture cycle and 1.60 g-inoculum size was the best one to hold the relatively stable anthocyanin production. The anthocyanin yield presented a negative correlation with intracellular sucrose content or intracellular total phosphate content.
Untapped sponge microbiomes: structure specificity at host order and family levels
Publisher: Oxford University Press (OUP)
Date: 23-08-2019
Abstract: Sponges are complex holobionts in which the structure of the microbiome has seldom been characterized above the host species level. The hypothesis tested in this study is that the structure of the sponge microbiomes is specific to the host at the order and family levels. This was done by using 33 sponge species belonging to 19 families representing five orders. A combination of three primer sets covering the V1-V8 regions of the 16S rRNA gene provided a more comprehensive coverage of the microbiomes. Both the ersity and structure of sponge microbiomes were demonstrated to be highly specific to the host phylogeny at the order and family levels. There are always dominant operational taxonomic units (OTUs) (relative abundance %) shared between microbial communities of sponges within the same family or order, but these shared OTUs showed high levels of dissimilarity between different sponge families and orders. The unique OTUs for a particular sponge family or order could be regarded as their ‘signature identity’. 70%–87% of these unique OTUs (class level) are unaffiliated and represent a vast resource of untapped microbiota. This study contributes to a deeper understanding on the concept of host-specificity of sponge microbiomes and highlights a hidden reservoir of sponge-associated microbial resources.
Neuroprotective activity of macroalgal fucofuroeckols against amyloid β peptide‐induced cell death and oxidative stress
Publisher: Wiley
Date: 25-04-2022
DOI: 10.1111/IJFS.15753
Abstract: Phlorotannins are polyphenolic compounds predominantly found in brown seaweeds tentatively identified as having neuroprotective bioactivity however, the effects of in idual constituent phlorotannins against amyloid β neurotoxicity, the main hallmark neurotoxic protein in Alzheimer’s disease (AD), is yet to be fully characterised. In this study, four phlorotannins, namely eckol, dieckol, phlorofucofuroeckol‐A (PFFA) and 974‐A sourced from the brown seaweed Ecklonia species were assessed for their ability to protect against the toxic effects of H 2 O 2 , lipid peroxidation via tert‐butyl hydroperoxide ( t ‐BHP) and Aβ 1–42 in neuronal PC12 cells. All compounds significantly scavenged reactive oxygen species (ROS). However, only PFFA and 974‐A protected PC12 cells from oxidative stress‐evoked neurotoxicity, providing significant increases in cell viability in response to both cytosolic (H 2 O 2) and lipid peroxidation‐evoked ( t ‐BHP) cell stress. None of the phlorotannins tested inhibited Aβ 1–42 aggregate morphology, which suggested that their neuroprotective activity was unrelated to direct interactions with Aβ 1–42 protein. Our results indicate that while all phlorotannins tested exhibited ROS scavenging activity, only fucofuroeckol‐type phlorotannins such as PFFA and 974‐A afforded broader neuroprotective activity in response to both oxidative stress and amyloid β exposure. The additional amyloid‐protective capacity of fucofuroeckols reveals the potential importance of the benzofuran moiety in neuroprotection and further studies are encouraged to investigate the chemico‐biological basis of this distinction in the search for neuroprotective therapies in dementia and other neurodegenerative conditions.
Comparative study on neuroprotective activities of fucoidans from Fucus vesiculosus and Undaria pinnatifida
Publisher: Elsevier BV
Date: 02-2019
DOI: 10.1016/J.IJBIOMAC.2018.10.168
Abstract: This study investigated the neuroprotective activities of five different fucoidan s les with different chemical compositions prepared from Fucus vesiculosus (FE, FF, and S) and Undaria pinnatifida (UE and UF) to determine if they reduced aggregation or cytotoxicity of Aβ
Kinetics of conventional and microwave-assisted fucoidan extractions from the brown alga, Ecklonia radiata
Publisher: Springer Science and Business Media LLC
Date: 31-10-2015
Experimental silicon demand by the sponge Hymeniacidon perlevis reveals chronic limitation in field populations
Publisher: Springer Science and Business Media LLC
Date: 04-01-2012
Critical assessment of quasi-steady-state method to determine effective diffusivities in alginate gel membranes
Publisher: Elsevier BV
Date: 09-1999
Increased lipid production of the marine oleaginous microalgae Isochrysis zhangjiangensis (Chrysophyta) by nitrogen supplement
Publisher: Elsevier BV
Date: 06-2011
DOI: 10.1016/J.BIORTECH.2011.04.006
Abstract: Effects of nitrate feeding on the cell growth and lipid accumulation of marine microalgae Isochrysis zhangjiangensis were investigated. When nitrate was supplied at interval of 24h, instead of 72 h, a high lipid content of 40.9% and a biomass density of 3.1 g L(-1) were obtained. To confirm whether I. zhangjiangensis accumulates lipid during nitrogen-repletion, a two-stage cultivation method was applied. This algal strain had a high lipid content during sustained nitrate addition and showed a high carbohydrate content under nitrate-depletion conditions. These results revealed that this algal strain can accumulate lipids under nitrogen-repletion conditions and accumulate carbohydrate under nitrogen-depletion conditions. When cultured in an extremely high nitrate concentration, 9 g L(-1) at 24h intervals, the growth of algal cells was suppressed, but the highest lipid content of 53% was attained. This special characteristic of lipid accumulation makes I. zhangjiangensis an ideal candidate for producing biodiesel using N-rich wastewater.
Application and optimization of the highly efficient and environmentally-friendly microwave-intensified lactic acid demineralization of deproteinized Rock lobster shells (Jasus edwardsii) for chitin production
Publisher: Elsevier BV
Date: 03-2017
Hyper-production of 13C-labeled trans-resveratrol in Vitis vinifera suspension cell culture by elicitation and in situ adsorption
Publisher: Elsevier BV
Date: 02-2011
Phlorotannins: A review on biosynthesis, chemistry and bioactivity
Publisher: Elsevier BV
Date: 02-2021
Intracellular ribonucleotide pools as a tool for monitoring the physiological state of plant cell suspension culture of Vitis vinifera in response to temperature change
Publisher: Elsevier BV
Date: 11-2009
Significant Enrichment of Polyunsaturated Fatty Acids (PUFAs) in the Lipids Extracted by Supercritical CO2 from the Livers of Australian Rock Lobsters (Jasus edwardsii)
Publisher: American Chemical Society (ACS)
Date: 05-05-2015
DOI: 10.1021/JF5059396
Abstract: Australian rock lobster (Jasus edwardsii) liver contains approximately 24.3% (w/w) lipids, which can contain a high amount of polyunsaturated fatty acids (PUFAs). However, this material has been found to be contaminated with arsenic (240 mg/kg) and cadmium (8 mg/kg). The high level of contaminants in the raw material and the large amount of PUFAs in the lipids prove a significant challenge in the extraction of high-quality lipids from this byproduct by conventional methods. Supercritical carbon dioxide (SC-CO2) extraction is a highly promising technology for lipid extraction with advantages including low contamination and low oxidation. The technique was optimized to achieve nearly 94% extraction of lipids relative to conventional Soxhlet extraction in Australian rock lobster liver at conditions of 35 MPa and 50 °C for 4 h. The extracted lipids are significantly enriched in PUFAs at 31.3% of total lipids, 4 times higher than those in the lipids recovered by Soxhlet extraction (7.8%). Specifically, the concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in SC-CO2 extraction are 7 times higher than those obtained by Soxhlet extraction. Moreover, very small amounts of toxic heavy metals such as lead (Pb), arsenic (As), mercury (Hg), and cadmium (Cd) were detected in the SC-CO2-extracted lipids, 0.5-27 times lower than those in the Soxhlet-extracted lipids, which are 40-200 times lower than the regulatory limit maximum values. The low levels of contaminants and the high proportion of PUFAs (dominated by DHA and EPA) found in the SC-CO2-extracted lipids from Australian rock lobster liver suggest that the material could potentially be used as a valuable source of essential fatty acids for human consumption.
Continuous flow fabrication of green graphene oxide in aqueous hydrogen peroxide
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D2NA00310D
Abstract: Herein, we have established a sequential two step continuous flow process for generating graphene oxide with properties comparable to the conventional Hummers' GO. The process is high yielding and with a dramatic reduction in the generation of waste.
A controlled aquarium system and approach to study the role of sponge-bacteria interactions using Aplysilla rosea and Vibrio natriegens
Publisher: Springer Science and Business Media LLC
Date: 07-08-2018
DOI: 10.1038/S41598-018-30295-Y
Abstract: Sponge-bacteria interactions are very important due to their ecological and biological significance. To understand the impact of interactions between sponges and bacteria (both associated with and external to sponges) on sponge-associated microbial ersity, sponge metabolite profiles and bioactivity, we used a controlled aquarium system and designed an experimental approach that allows the study of sponge-bacteria interactions in a well-defined manner. To test the feasibility of this approach, this system was used to study the interaction between a sponge Aplysilla rosea and a marine bacterium commonly found in seawater, Vibrio natriegens . Sponge explants were exposed to V . natriegens , at 5 × 10 6 cfu/ml, and changes were monitored for 48 hours. Pyro-sequencing revealed significant shifts in microbial communities associated with the sponges after 24 to 48 hours. Both the control (sponge only without added bacteria) and Vibrio -exposed sponges showed a distinct shift in bacterial ersity and abundance with time. Vibrio exposure significantly increased bacterial ersity, the abundance of a number of taxa compared to control sponges. The result experimentally supports the notion of dynamic and concerted responses by the sponge when interacting with a bacterium, and demonstrates the feasibility of using this controlled aquarium system for the study of sponge-bacteria interactions.
Turbo thin film continuous flow production of biodiesel from fungal biomass
Publisher: Elsevier BV
Date: 02-2019
DOI: 10.1016/J.BIORTECH.2018.11.015
Abstract: Direct biodiesel production from wet fungal biomass may significantly reduce production costs, but there is a lack of fast and cost-effective processing technology. A novel thin film continuous flow process has been applied to study the effects of its operational parameters on fatty acid (FA) extraction and FA to fatty acid methyl ester (FAME) conversion efficiencies. Single factor experiments evaluated the effects of catalyst concentration and water content of biomass, while factorial experimental designs determined the interactions between catalyst concentration and biomass to methanol ratio, flow rate, and rotational speed. Direct transesterification (DT) of wet Mucor plumbeus biomass at ambient temperature and pressure achieved a FA to FAME conversion efficiency of >90% using 3 wt/v % NaOH concentration, if the water content was ≤50% (w/w). In comparison to existing DT methods, this continuous flow processing technology has an estimated 90-94% reduction in energy consumption, showing promise for up-scaling.
Actinoalloteichus hymeniacidonis sp. nov., an actinomycete isolated from the marine sponge Hymeniacidon perleve
Publisher: Microbiology Society
Date: 10-2006
Abstract: A polyphasic study was undertaken to establish the taxonomic status of a Gram-positive, aerobic actinomycete, strain HPA177 T , isolated from a marine sponge, Hymeniacidon perleve . The organism formed branching, non-fragmenting vegetative hyphae and produced black pigment. Chemotaxonomic characteristics were consistent with its assignment to the genus Actinoalloteichus . Analysis of the 16S rRNA gene sequence showed that strain HPA177 T formed a robust clade with type strains of the genus Actinoalloteichus , but was distinct from them. A number of phenotypic characteristics also readily distinguished strain HPA177 T from species of the genus Actinoalloteichus with validly published names. On the basis of the above data, it is proposed that strain HPA177 T represents a novel species, Actinoalloteichus hymeniacidonis sp. nov. The type strain of Actinoalloteichus hymeniacidonis is HPA177 T (=CGMCC 4.2500 T =JCM 13436 T ).
Oryzamides A–E, Cyclodepsipeptides from the Sponge-Derived Fungus Nigrospora oryzae PF18
Publisher: American Chemical Society (ACS)
Date: 04-08-2016
DOI: 10.1021/ACS.JNATPROD.6B00349
Abstract: Three new cyclohexadepsipeptides, oryzamides A-C (1-3), two isolation artifacts, oryzamides D (4) and E (5), and the known congener scopularide A (6), all possessing a rare 3-hydroxy-4-methyldecanoic acid (HMDA) substructure, were isolated from the mycelial extract of the sponge-derived fungus Nigrospora oryzae PF18. Their planar structures were elucidated by spectroscopic analysis and comparison with the literature data. The absolute configurations were determined using the advanced Marfey's method and single-crystal X-ray diffraction analysis. Among them, oryzamides D (4) and E (5) were a pair of diastereomers at the sulfur atom of the l-methionine sulfoxide residue, which showcased the possible separation of a pair of methionine sulfoxide diastereomers. The X-ray crystal structure of scopularide A (6) was obtained for the first time, thereby establishing its relative and absolute configuration at C-4 of the HMDA residue. Oryzamides A-C (1-3) did not display cytotoxic, antibacterial, antiparasitic, and NF-κB inhibitory activities.
Identification of Antioxidative Peptides Derived from Arthrospira maxima in the Biorefinery Process after Extraction of C-Phycocyanin and Lipids
Publisher: MDPI AG
Date: 24-02-2023
DOI: 10.3390/MD21030146
Abstract: Arthrospira maxima has been identified as a sustainable source of rich proteins with erse functionalities and bioactivities. After extracting C-phycocyanin (C-PC) and lipids in a biorefinery process, the spent biomass still contains a large proportion of proteins with potential for biopeptide production. In this study, the residue was digested using Papain, Alcalase, Trypsin, Protamex 1.6, and Alcalase 2.4 L at different time intervals. The resulting hydrolyzed product with the highest antioxidative activity, evaluated through their scavenging capability of hydroxyl radicals, superoxide anion, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), was selected for further fractionation and purification to isolate and identify biopeptides. Alcalase 2.4 L was found to produce the highest antioxidative hydrolysate product after four-hour hydrolysis. Fractionating this bioactive product using ultrafiltration obtained two fractions with different molecular weights (MW) and antioxidative activity. The low-molecular-weight fraction (LMWF) with MW kDa had higher DPPH scavenging activity with the IC50 value of 2.97 ± 0.33 compared to 3.76 ± 0.15 mg/mL of the high-molecular-weight fraction (HMWF) with MW kDa. Two stronger antioxidative fractions (F-A and F-B) with the respective significant lower IC50 values of 0.83 ± 0.22 and 1.52 ± 0.29 mg/mL were isolated from the LMWF using gel filtration with a Sephadex G-25 column. Based on LC-MS/MS analysis of the F-A, 230 peptides derived from 108 A. maxima proteins were determined. Notably, different antioxidative peptides possessing various bioactivities, including antioxidation, were detected with high predicted scores together with in silico analyses on their stability and toxicity. This study established knowledge and technology to further value-add to the spent A. maxima biomass by optimizing hydrolysis and fraction processes to produce antioxidative peptides with Alcalase 2.4 L after two products already produced in a biorefinery. These bioactive peptides have potential applications in food and nutraceutical products.
A two-phase flow model coupling with volume of fluid and immersed boundary methods for free surface and moving structure problems
Publisher: Elsevier BV
Date: 12-2013
Investigation of photoinduced electron transfer based on immobilized Green microalga Tetraselmis subcordiformis photoanode
Publisher: IEEE
Date: 08-2013
A combination of elicitation and precursor feeding leads to increased anthocyanin synthesis in cell suspension cultures of Vitis vinifera
Publisher: Springer Science and Business Media LLC
Date: 29-05-2011
Cytotoxic effect of xanthones from pericarp of the tropical fruit mangosteen (Garcinia mangostana Linn.) on human melanoma cells
Publisher: Elsevier BV
Date: 09-2011
DOI: 10.1016/J.FCT.2011.06.051
Abstract: Mangosteen (Garcinia mangostana Linn.) is a tropical tree from South East Asia and its fruit pericarp is a well-known traditional medicine. In this study, the cytotoxic effect of three xanthone compounds (α-mangostin, γ-mangostin, and 8-deoxygartanin) from mangosteen pericarp was investigated using the human melanoma SK-MEL-28 cell line. Significant dose-dependent reduction in % cell viability was induced. γ-Mangostin and 8-deoxygartanine at 5 μg/ml increased the cell cycle arrest in G(1) phase (90% and 92%) compared with untreated cells (78%). All compounds induced apoptosis, of the highest being α-mangostin at 7.5 μg/ml that induced 59.6% early apoptosis, compared to 1.7% in untreated cells. The apoptotic effect of α-mangostin was via caspase activation and disruption of mitochondrial membrane pathways as evidenced by 25-fold increased caspase-3 activity and 9-fold decreased mitochondrial membrane potential when compared to untreated cells. In conclusion, these xanthones, especially α-mangostin, are potential candidates as anti-melanoma agents.
Preparative separation of major xanthones from mangosteen pericarp using high‐performance centrifugal partition chromatography
Publisher: Wiley
Date: 30-04-2010
Abstract: Mangosteen fruit pericarp (MFP) is a rich source of xanthones, which has shown remarkable pharmacological activities. To isolate xanthones, previous methods included labor intensive and time-consuming solid-phase extractions (Sephadex LH20, silica gel) and sequential solvent extraction. In this study, major xanthones (alpha- and gamma-mangostins) in MFP were isolated at high purity in one step utilizing high-performance centrifugal partition chromatography with solvent system composed of petroleum ether, ethyl acetate, methanol and water (10:5:5:1). In one run, 200 mg crude extract of MFP was injected and 55.4 mg alpha-mangostin and 12.4 mg gamma-mangostin were obtained with the purity of 93.6 and 98.4%, respectively. The yields of them were 86.3 and 76.3%, respectively. As alpha- and gamma-mangostins are reported to show potent antioxidant, anti-inflammatory and anticancer activities, this method can be used for the large-scale production of them for future in vitro and in vivo biological studies.
Vortex fluidic mediated direct transesterification of wet microalgae biomass to biodiesel
Publisher: Elsevier BV
Date: 10-2018
DOI: 10.1016/J.BIORTECH.2018.06.103
Abstract: A bottleneck in the production of biodiesel from microalgae is the dewatering and lipid extraction process which is the dominant energy penalty and cost. A novel biodiesel production platform based on vortex fluidic device (VFD)-assisted direct transesterification (DT) of wet microalgal biomass of Chloroparva pannonica was developed and evaluated. Fatty acid extraction and fatty acid to FAME conversion efficiencies were used at different parameter settings to evaluate performance of the processing technology in confined and continuous mode. A response surface method based on Box-Behnken experimental design was used to determine the effects of water content, the ratio of biomass to methanol and residence time in the VFD. Average extraction efficiencies were 41% and conversion efficiencies >90% with the processing technology showing a broad tolerance to parameter settings. The findings suggest that VFD-assisted DT is a simple and effective way to produce biodiesel directly from wet microalgae biomass at room temperature.
Significantly improved taxuyunnanine C production in cell suspension cultures of Taxus chinensis by process intensification of repeated elicitation, sucrose feeding, and in situ adsorption
Publisher: Springer Science and Business Media LLC
Date: 18-02-2011
New marine natural products from sponges (Porifera) of the order Dictyoceratida (2001 to 2012); a promising source for drug discovery, exploration and future prospects
Publisher: Elsevier BV
Date: 09-2016
DOI: 10.1016/J.BIOTECHADV.2015.12.008
Abstract: The discovery of new drugs can no longer rely primarily on terrestrial resources, as they have been heavily exploited for over a century. During the last few decades marine sources, particularly sponges, have proven to be a most promising source of new natural products for drug discovery. This review considers the order Dictyoceratida in the Phylum Porifera from which the largest number of new marine natural products have been reported over the period 2001-2012. This paper examines all the sponges from the order Dictyoceratida that were reported as new compounds during the time period in a comprehensive manner. The distinctive physical characteristics and the geographical distribution of the different families are presented. The wide structural ersity of the compounds produced and the variety of biological activities they exhibited is highlighted. As a representative of sponges, insights into this order and avenues for future effective natural product discovery are presented. The research institutions associated with the various studies are also highlighted with the aim of facilitating collaborative relationships, as well as to acknowledge the major international contributors to the discovery of novel sponge metabolites. The order Dictyoceratida is a valuable source of novel chemical structures which will continue to contribute to a new era of drug discovery.
Analysis of actinobacteria communities in marine sponge by use of clone library with different pcr primers
Publisher: Elsevier BV
Date: 11-2010
Discovery of antimicrobial activities of a marine diatom Thalassiosira rotula
Publisher: Academic Journals
Date: 31-12-2013
DOI: 10.5897/AJMR12.2183
Comparative analysis of sugar and mineral content of Sargassum spp. collected from different coasts of Sri Lanka
Publisher: Springer Science and Business Media LLC
Date: 05-03-2019
Vortex Fluidic Device-Intensified Aqueous Two Phase Extraction of C-Phycocyanin from Spirulina maxima
Publisher: American Chemical Society (ACS)
Date: 03-06-2016
Cloning and structure analysis of hydrogenase gene from Chlamydomonas reinhardtii SE
Publisher: Elsevier BV
Date: 09-2005
Optimisation of biorefinery production of alginate, fucoidan and laminarin from brown seaweed Durvillaea potatorum
Publisher: Elsevier BV
Date: 03-2019
Analysis of metal elements of hydrangea sepals at various growing stages by ICP-AES
Publisher: Elsevier BV
Date: 06-2003
Isolation, characterization and identification of a Paracoccus sp. 2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis
Publisher: Wiley
Date: 07-02-2011
Abstract: A 2-haloacid dehalogenase-producing bacterium, designated DEH99, was isolated from the marine sponge Hymeniacidon perlevis using a modified enrichment medium and a pH indicator method. DEH99 could degrade only half of the racemic mixture 2-chloropropionic acid (2-CPA) in the medium. The dehalogenase of DEH99 was further determined to be a (S)-2-haloacid dehalogenase, which can degrade 2-CPA, 2-bromopropionic acid (2-BPA), and iodoacetic acid. The gene encoding the (S)-2-haloacid dehalogenase was partially sequenced and classified into the Group II family. The 2-haloacid dehalogenase showed the highest sequence similarity (77% with 21% query coverage) to the haloacid dehalogenase (dhlB) gene of Xanthobacter autotrophicus. A phylogenetic analysis of the 16S rDNA sequence demonstrated that the isolate DEH99 is a member of the genus Paracoccus. To our knowledge, this is the first report detailing the isolation of a strain of genus Paracoccus having 2-haloacid dehalogenase activity from marine sponges.
Primmorphs from archaeocytes-dominant cell population of the spongehymeniacidon perleve: improved cell proliferation and spiculogenesis
Publisher: Wiley
Date: 17-10-2003
DOI: 10.1002/BIT.10811
Abstract: Marine sponges (Porifera) possess an extraordinary ersity of bioactive metabolites for new drug discovery and development. In vitro cultivation of sponge cells in a bioreactor system is very attractive for the sustainable production of sponge-derived bioactive metabolites however, it is still a challenging task. The recent establishment of sponge primmorphs, multicellular aggregates from dissociated mixed-cell population (MCP), has been widely acknowledged to hold great promise for cultivation in vitro. Here we present a new method to establish an in vitro sponge primmorph culture from archaeocyte-dominant cell population (ADCP) enriched by a Ficoll gradient, rather than a mixed-cell population (MCP). Our rationale is based upon the totipotency (the ability of a cell to differentiate into other cell types) of archaeocyte cells and the different biological functions of various sponge cell types. A sponge, Hymeniacidon perleve collected from the China Yellow Sea was used as a model system for this investigation. Distinct dynamics of primmorph formation were observed while significant increases in DNA synthesis, cell proliferation (up to threefold), and cell growth (up to fourfold) were achieved. Furthermore, a time-dependent spiculogenesis was clearly demonstrated in our longterm culture, indicating high metabolic activity of primmorphs from the ADCP. This new method represents an important step forward to advance sponge cell culture in vitro that may lead to commercial exploitation of sponge-derived drugs.
Selective Growth Inhibition of Human Leukemia and Human Lymphoblastoid Cells by Resveratrol via Cell Cycle Arrest and Apoptosis Induction
Publisher: American Chemical Society (ACS)
Date: 26-07-2008
DOI: 10.1021/JF801014P
Abstract: There is great interest in the potential chemopreventive activity of resveratrol against human cancers. However, there are conflicting results on its growth inhibitory effect on normal cells. This project examined the differential effect of resveratrol at physiologically relevant concentrations on nonmalignant (WIL2-NS) and malignant (HL-60) cell lines and compared the underlying mechanisms via cell cycle modulation, apoptosis induction, and genotoxicity potential. Twenty-four hours of exposure to resveratrol was toxic to WIL2-NS and HL-60 cells in a dose-dependent manner. WIL2-NS cells regrew 5 times more than HL-60 cells by 120 h after the removal of 100 microM resveratrol (p < 0.05). Furthermore, significant alterations in cell cycle kinetics were induced by resveratrol in HL-60 cells, but were to a lesser extent for WIL2-NS cells. The proportion of apoptosis was also 3 times higher in HL-60 cells as compared to WIL2-NS cells for 100 microM resveratrol (p < 0.05). In conclusion, resveratrol preferentially inhibited the growth of HL-60 cells via cell cycle modulation and apoptosis induction and subsequently directed the cells to irreversible cell death, whereas the effect on WIL2-NS cells was largely reversible.
Advances in Microalgae-Derived Phytosterols for Functional Food and Pharmaceutical Applications
Publisher: MDPI AG
Date: 09-07-2015
DOI: 10.3390/MD13074231
Bioactives From Marine Animals: Potential Benefits for Human Reproductive Health
Publisher: Frontiers Media SA
Date: 30-03-2022
DOI: 10.3389/FMARS.2022.872775
Abstract: Reproductive and sexual health issues, including infertility and sexual dysfunctions (SD), are common concerns affecting millions of reproductive age worldwide. Scattered literature reports that marine animals such as oysters, sea cucumbers, seahorses and spoon worms have unique bioactive compounds like saponins, steroids, seahorse-derived hydrolysates, polypeptide, oligopeptides and essential trace elements that significantly improve infertility, hormonal imbalance, SD, and impotence. In addition, these compounds have exhibited pharmacological properties against reproductive problems due to diabetes and exposure to electromagnetic fields, cyclophosphamide, or a high exercise load. This review presents the first critical assessment of the advances in understanding and applying bioactives from marine organisms to support human reproductive health. Key knowledge and technical gaps have been identified for future research to improve the lack of in-depth understanding of the mechanism and action of these bioactives in human clinical studies. There is a need to develop simple, selective, low-cost, and scalable processes to isolate and purify in idual bioactive compounds for industrial applications. The optimizing culturing and farming conditions for specific bioactive compounds from targeted species are suggested for sustainable production. The review indicates a promising future of extracts and marine-derived bioactives as functional foods in preventing and managing human reproductive health issues, but mechanistic studies and further clinical trials are urgently required to evaluate their efficacy and safety.
Protein Recovery from Underutilised Marine Bioresources for Product Development with Nutraceutical and Pharmaceutical Bioactivities
Publisher: MDPI AG
Date: 27-07-2020
DOI: 10.3390/MD18080391
Abstract: The global demand for dietary proteins and protein-derived products are projected to dramatically increase which cannot be met using traditional protein sources. Seafood processing by-products (SPBs) and microalgae are promising resources that can fill the demand gap for proteins and protein derivatives. Globally, 32 million tonnes of SPBs are estimated to be produced annually which represents an inexpensive resource for protein recovery while technical advantages in microalgal biomass production would yield secure protein supplies with minimal competition for arable land and freshwater resources. Moreover, these biomaterials are a rich source of proteins with high nutritional quality while protein hydrolysates and biopeptides derived from these marine proteins possess several useful bioactivities for commercial applications in multiple industries. Efficient utilisation of these marine biomaterials for protein recovery would not only supplement global demand and save natural bioresources but would also successfully address the financial and environmental burdens of biowaste, paving the way for greener production and a circular economy. This comprehensive review analyses the potential of using SPBs and microalgae for protein recovery and production critically assessing the feasibility of current and emerging technologies used for the process development. Nutritional quality, functionalities, and bioactivities of the extracted proteins and derived products together with their potential applications for commercial product development are also systematically summarised and discussed.
Fish Protein Hydrolysates: Application in Deep-fried Food and Food Safety Analysis
Publisher: Wiley
Date: 2015
Abstract: Four different processes (enzymatic, microwave-intensified enzymatic, chemical, and microwave-intensified chemical) were used to produce fish protein hydrolysates (FPH) from Yellowtail Kingfish for food applications. In this study, the production yield and oil-binding capacity of FPH produced from different processes were evaluated. Microwave intensification significantly increased the production yields of enzymatic process from 42% to 63%. It also increased the production yields of chemical process from 87% to 98%. The chemical process and microwave-intensified chemical process produced the FPH with low oil-binding capacity (8.66 g oil/g FPH and 6.25 g oil/g FPH), whereas the microwave-intensified enzymatic process produced FPH with the highest oil-binding capacity (16.4 g oil/g FPH). The FPH from the 4 processes were applied in the formulation of deep-fried battered fish and deep-fried fish cakes. The fat uptake of deep-fried battered fish can be reduced significantly from about 7% to about 4.5% by replacing 1% (w/w) batter powder with FPH, and the fat uptake of deep-fried fish cakes can be significantly reduced from about 11% to about 1% by replacing 1% (w/w) fish mince with FPH. Food safety tests of the FPH produced by these processes demonstrated that the maximum proportion of FPH that can be safely used in food formulation is 10%, due to its high content of histamine. This study demonstrates the value of FPH to the food industry and bridges the theoretical studies with the commercial applications of FPH.
Distribution of Saponins in the Sea Cucumber Holothuria lessoni
Publisher: MDPI AG
Date: 11-2018
DOI: 10.3390/MD16110423
Abstract: Sea cucumbers are an important ingredient of traditional folk medicine in many Asian countries, which are well-known for their medicinal, nutraceutical, and food values due to producing an impressive range of distinctive natural bioactive compounds. Triterpene glycosides are the most abundant and prime secondary metabolites reported in this species. They possess numerous biological activities ranging from anti-tumour, wound healing, hypolipidemia, pain relieving, the improvement of nonalcoholic fatty livers, anti-hyperuricemia, the induction of bone marrow hematopoiesis, anti-hypertension, and cosmetics and anti-ageing properties. This study was designed to purify and elucidate the structure of saponin contents of the body wall of sea cucumber Holothuria lessoni and to compare the distribution of saponins of the body wall with that of the viscera. The body wall was extracted with 70% ethanol, and purified by a liquid-liquid partition chromatography, followed by isobutanol extraction. A high-performance centrifugal partition chromatography (HPCPC) was conducted on the saponin-enriched mixture to obtain saponins with a high purity. The resultant purified saponins were analyzed using MALDI-MS/MS and ESI-MS/MS. The integrated and hyphenated MS and HPCPC analyses revealed the presence of 89 saponin congeners, including 35 new and 54 known saponins, in the body wall in which the majority of glycosides are of the holostane type. As a result, and in conjunction with existing literature, the structure of four novel acetylated saponins, namely lessoniosides H, I, J, and K were characterized. The identified triterpene glycosides showed potent antifungal activities against tested fungi, but had no antibacterial effects on the bacterium Staphylococcus aureus. The presence of a wide range of saponins with potential applications is promising for cosmeceutical, medicinal, and pharmaceutical products to improve human health.
Significant enhancement of photobiological H2evolution by carbonylcyanide m-chlorophenylhydrazone in the marine green alga Platymonas subcordiformis
Publisher: Springer Science and Business Media LLC
Date: 07-2004
Marine Sponge Endosymbionts: Structural and Functional Specificity of the Microbiome within Euryspongia arenaria Cells
Publisher: American Society for Microbiology
Date: 29-06-2022
DOI: 10.1128/SPECTRUM.02296-21
Abstract: Sponges host abundant microbial symbionts that can produce an impressive number of novel bioactive metabolites. However, knowledge on intracellular (endosymbiotic) microbiota is scarce.
Subcellular Localization and Identification of Hydrogenase Isolated from the Marine Green Alga Platymonas subcordiformis Using Immunoprecipitation and MALDI-TOF MS
Publisher: Elsevier BV
Date: 03-2007
DOI: 10.1016/S1872-2075(07)60027-2
Abstract: A marine unicellular green alga, Platymonas subcordiformis, was demonstrated to photobiologically produce hydrogen gas from seawater. The objective of this study was to localize and identify the hydrogenase isolated from P. subcordiformis. Adaptation in the presence of inhibitors of protein biosynthesis indicated that the hydrogenase was much more inhibited by cycloheximide than that by chlor henicol. The result suggested that the hydrogenase isolated from P. subcordiformis is probably synthesized in cytoplasmic ribosomes. Both Western blot analysis and immunogold electron microscopy demonstrate that the P. subcordiformis hydrogenase is mainly located in the chloroplast stroma. The proteins that reacted specifically with the antibodies against the iron hydrogenase isolated from Chlamydomonas reinhardtii were concentrated by immunoprecipitation. The separated protein bands were cut out of the SDS-PAGE gel, in-gel digested by trypsin, and analyzed by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). Mascot was employed for analysis of the MALDI data using the public databases NCBInr. The hydrogenase isolated from P. subcordiformis was identified to be the Fe-hydrogenase.
Magnetite Nanoparticle/Copper Phosphate Nanoflower Composites for Fenton-like Organic Dye Degradation
Publisher: American Chemical Society (ACS)
Date: 25-01-2022
Corrigendum to “An efficient protein isolation process for use in Limnospira maxima
Publisher: Elsevier BV
Date: 2023
Biopotentials of marine sponges from China oceans: past and future
Publisher: Elsevier BV
Date: 07-2003
DOI: 10.1016/S1389-0344(03)00066-2
Abstract: An extensive literature survey of over 17 Journals was carried out on Chinese sponges and their natural products in the period from 1980 to 2001. This review is thus intended to provide the first thorough overview of research on marine sponges from China Ocean territories. Information is provided about the rather-limited taxonomic study of Chinese marine sponges, with an analysis on their distribution and ersity. Research findings on the natural products and their bioactivity screening from Chinese sponges are summarized. The weaknesses, gaps and problems in the past R&D program of Chinese sponges are identified, which point to the future opportunities in exploiting these huge untapped sponge resources. The report is expected to serve as an entry point for understanding Chinese sponges and for furthering R&D on their bioactive compounds for new drug development.
Characterization of hydrogen production by Platymonas Subcordiformis in torus photobioreactor
Publisher: Elsevier BV
Date: 07-2010
Dysiscalarones A-E, scalarane sesterterpenoids with nitric oxide production inhibitory activity from marine sponge Dysidea granulosa
Publisher: Elsevier BV
Date: 06-2021
A Two-Phase Flow Model with VOF for Free Surface Flow Problems
Publisher: Trans Tech Publications, Ltd.
Date: 11-2012
DOI: 10.4028/WWW.SCIENTIFIC.NET/AMM.232.279
Abstract: A numerical model based on the two-phase flow model for incompressible viscous fluid with a complex free surface has been developed in this study. The two-step projection method is employed to solve the Navier–Stokes equations in the numerical solutions, and finite difference method on a staggered grid is used throughout the computation. The two-order accurate volume of fluid (VOF) method is used to track the distorted and broken free surfaces. The two-phase model is first validated by simulating the dam break over a dry bed, in which the numerical results and experimental data agree well. Then 2-D fluid sloshing in a horizontally excited rectangular tank at different excitation frequencies is simulated using this two-phase model. The results of this study show that the two-phase flow model with VOF method is a potential tool for the simulation of nonlinear fluid sloshing. These studies demonstrate the capability of the two-phase model to simulate free surface flow problems with considering air movement effects.
A normal mucin-binding lectin from the sponge Craniella australiensis
Publisher: Elsevier BV
Date: 05-2006
DOI: 10.1016/J.CBPC.2005.11.008
Abstract: A lectin, Craniella australiensis (CAL), was isolated from sponge C. australiensis by ion-exchange on DEAE-Sephacel and purified by gel filtration on Sephadex G-150 and HPLC on DEAE-5PW. The purified lectin was a trimeric protein as revealed by SDS-PAGE and MALDI-TOF analysis. SDS-PAGE showed that the CAL protein had a molecular mass of 54 kDa, and consisted of three 18 kDa subunits. Gel filtration of purified lectin on Sephadex G-200 indicates that it exists as a 54 kDa protein in its native state. The amino acid composition was rich in Thr and Glx. CAL was found to agglutinate native and trypsinized human A, B erythrocytes, and agglutinate native erythrocytes of mouse, sheep, rabbit and chicken, and trypsinized erythrocytes of sheep and rabbit. The hemagglutination activity was inhibited by glycoproteins such as PSM and asialo-PSM, but not by any of the monosaccharides tested. The activity was stable between 20 and 70 degrees C. Significant CAL activity was observed between pH 5 and 8. The lectin reaction is independent of the presence of alent cations Ca2+ and Mg2+. The sequence of N-terminal residues of CAL was determined as TSSCQSIVVE. The lectin showed a potent mitogenic response towards BALB/c splenocytes.
A type of characteristic peroxisome in the spherulous cells of the marine sponge Axinella sp.
Publisher: Elsevier BV
Date: 11-2010
Ecklonia radiata extract containing eckol protects neuronal cells against Aβ1–42 evoked toxicity and reduces aggregate density
Publisher: Royal Society of Chemistry (RSC)
Date: 2020
DOI: 10.1039/D0FO01438A
Abstract: Ecklonia radiata extract and phlorotannin fraction containing predominantly eckol-type phlorotannins inhibited the cytotoxicity of Aβ 1–42 in neuronal PC-12 cells and evoked a reduction in the density of Aβ 1–42 aggregates.
Vortex fluidic induced mass transfer across immiscible phases
Publisher: Royal Society of Chemistry (RSC)
Date: 2022
DOI: 10.1039/D1SC05829K
Abstract: Micron to submicron size Coriolis and Faraday wave induced high shear topological flow regimes in 45° titled rapidly rotating tubes result in high inter-phase mass transfer of immiscible liquids and spontaneous demixing.
Interspecies interactions in bacterial colonies are determined by physiological traits and the environment
Publisher: Cold Spring Harbor Laboratory
Date: 30-04-2019
DOI: 10.1101/623017
Abstract: Interspecies interactions in bacterial biofilms have important impacts on the composition and function of communities in natural and engineered systems. To investigate these interactions, synthetic communities provide experimentally tractable systems. Agar-surface colonies are similar to biofilms and have been used for investigating the eco-evolutionary and biophysical forces that determine community composition and spatial distribution of bacteria. Prior work has focused on intraspecies interactions, using differently fluorescent tagged but identical or genetically modified strains of the same species. Here, we investigated how physiological differences determine the community composition and spatial distribution in synthetic communities of Pseudomonas aeruginosa , Pseudomonas protegens and Klebsiella pneumoniae . Using quantitative microscopic imaging, we found that interspecies interactions in multispecies colonies are influenced by type IV pilus mediated motility, extracellular matrix secretion, environmental parameters and the specific species involved. These results indicate that the patterns observable in mixed species colonies can be used to understand the mechanisms that drive interspecies interactions, which are dependent on the interplay between specific species’ physiology and environmental conditions.
Microbial and Plant Cell Synthesis of Secondary Metabolites and Strain Improvement
Publisher: CRC Press
Date: 12-12-2011
DOI: 10.1201/B11490-11
To Stretch the Boundary of Secondary Metabolite Production in Plant Cell-Based Bioprocessing: Anthocyanin as a Case Study.
Publisher: Hindawi Limited
Date: 2004
DOI: 10.1155/S1110724304404148
Abstract: Plant cells and tissue cultures hold great promise for controlled production of a myriad of useful secondary metabolites on demand. The current yield and productivity cannot fulfill the commercial goal of a plant cell-based bioprocess for the production of most secondary metabolites. In order to stretch the boundary, recent advances, new directions and opportunities in plant cell-based bioprocessing, have been critically examined for the 10 years from 1992 to 2002. A review of the literature indicated that most of the R& D work was devoted predominantly to studies at an empirical level. A rational approach to molecular plant cell bioprocessing based on the fundamental understanding of metabolic pathways and their regulations is urgently required to stimulate further advances however, the strategies and technical framework are still being developed. It is the aim of this review to take a step forward in framing workable strategies and technologies for molecular plant cell-based bioprocessing. Using anthocyanin biosynthesis as a case study, an integrated postgenomic approach has been proposed. This combines the functional analysis of metabolic pathways for biosynthesis of a particular metabolite from profiling of gene expression and protein expression to metabolic profiling. A global correlation not only can thus be established at the three molecular levels, but also places emphasis on the interactions between primary metabolism and secondary metabolism between competing and/or complimentary pathways and between biosynthetic and post-biosynthetic events.
Purification and characterization of a hydrogenase from the marine green alga Tetraselmis subcordiformis
Publisher: Elsevier BV
Date: 05-2011
The role of sponge-bacteria interactions: the sponge Aplysilla rosea challenged by its associated bacterium Streptomyces ACT-52A in a controlled aquarium system
Publisher: Springer Science and Business Media LLC
Date: 07-10-2016
DOI: 10.1007/S00253-016-7878-9
Abstract: Sponge-associated bacteria play a critical role in sponge biology, metabolism and ecology, but how they interact with their host sponges and the role of these interactions are poorly understood. This study investigated the role of the interaction between the sponge Aplysilla rosea and its associated actinobacterium, Streptomyces ACT-52A, in modifying sponge microbial ersity, metabolite profile and bioactivity. A recently developed experimental approach that exposes sponges to bacteria of interest in a controlled aquarium system was improved by including the capture and analysis of secreted metabolites by the addition of an absorbent resin in the seawater. In a series of controlled aquaria, A. rosea was exposed to Streptomyces ACT-52A at 10
New diterpene alkaloids from the marine sponge Agelas mauritiana
Publisher: Royal Society of Chemistry (RSC)
Date: 2017
DOI: 10.1039/C7RA02547E
Industrial biotechnology: Current status and future development for the sustainability of human society
Publisher: Elsevier BV
Date: 10-2009
The protective effects of XH‐105 against radiation‐induced intestinal injury
Publisher: Wiley
Date: 20-01-2019
DOI: 10.1111/JCMM.14159
Multiple-response optimization of the acidic treatment of the brown alga Ecklonia radiata for the sequential extraction of fucoidan and alginate
Publisher: Elsevier BV
Date: 12-2015
DOI: 10.1016/J.BIORTECH.2015.08.103
Abstract: The aim of this study was to optimize the acidic treatment of the brown alga Ecklonia radiata in order to extract fucoidan and facilitate the efficient sequential extraction of alginates. Response surface methodology was used to determine the effects of the temperature, pH, and duration of the acidic treatment on fucoidan yield, alginate extractability, and the molecular weight of sequentially extracted alginates. Desirability functions were then used to predict the best overall combinations of responses. The most desirable compromise allowed for the recovery of a fucoidan-rich fraction with a yield of 3.75% (w/w of alga) and the sequential extraction of alginates having an average molecular weight of 730kDa at a yield of 44% (w/w of alga), with low cross-contamination between the products. The optimized acidic treatment could form the basis of an industrial biorefinery process for the production of both fucoidan and alginate.
Effects of Calcium and Vanadium Ions on the Epoxidation of Cyclohexene with H<SUB>2</SUB>O<SUB>2</SUB> Catalyzed by Vanadium Bromoperoxidase
Publisher: China Science Publishing & Media Ltd.
Date: 12-2010
Sponge-associated actinobacterial diversity: validation of the methods of actinobacterial DNA extraction and optimization of 16S rRNA gene amplification
Publisher: Springer Science and Business Media LLC
Date: 07-08-2015
DOI: 10.1007/S00253-015-6875-8
Abstract: Experiments were designed to validate the two common DNA extraction protocols (CTAB-based method and DNeasy Blood & Tissue Kit) used to effectively recover actinobacterial DNA from sponge s les in order to study the sponge-associated actinobacterial ersity. This was done by artificially spiking sponge s les with actinobacteria (spores, mycelia and a combination of the two). Our results demonstrated that both DNA extraction methods were effective in obtaining DNA from the sponge s les as well as the sponge s les spiked with different amounts of actinobacteria. However, it was noted that in the presence of the sponge, the bacterial 16S rRNA gene could not be lified unless the combined DNA template was diluted. To test the hypothesis that the extracted sponge DNA contained inhibitors, dilutions of the DNA extracts were tested for six sponge species representing five orders. The results suggested that the inhibitors were co-extracted with the sponge DNA, and a high dilution of this DNA was required for the successful PCR lification for most of the s les. The optimized PCR conditions, including primer selection, PCR reaction system and program optimization, further improved the PCR performance. However, no single PCR condition was found to be suitable for the erse sponge s les using various primer sets. These results highlight for the first time that the DNA extraction methods used are effective in obtaining actinobacterial DNA and that the presence of inhibitors in the sponge DNA requires high dilution coupled with fine tuning of the PCR conditions to achieve success in the study of sponge-associated actinobacterial ersity.
Uncovering the hidden marine sponge microbiome by applying a multi-primer approach
Publisher: Springer Science and Business Media LLC
Date: 17-04-2019
DOI: 10.1038/S41598-019-42694-W
Abstract: Marine sponges (phylum Porifera) are hosts to microorganisms that make up to 40–60% of the mesohyl volume. The challenge is to characterise this microbial ersity more comprehensively. To accomplish this, a new method was for the first time proposed to obtain sequence coverage of all the variable regions of the 16S rRNA gene to analyze the licon-based microbiomes of four representative sponge species belonging to different orders. The five primer sets targeting nine variable regions of the 16S rRNA gene revealed a significant increase in microbiome coverage of 29.5% of phylum level OTUs and 35.5% class level OTUs compared to the community revealed by the commonly used V4 region-specific primer set alone. Among the resulting OTUs, 52.6% and 61.3% were unaffiliated, including candidate OTUs, at the phylum and class levels, respectively, which demonstrated a substantially superior performance in uncovering taxonomic ‘blind spots’. Overall, a more complete sponge microbiome profile was achieved by this multi-primer approach, given the significant improvement of microbial taxonomic coverage and the enhanced capacity to uncover novel microbial taxa. This multi-primer approach represents a fundamental and practical change from the conventional single primer set licon-based microbiome approach, and can be broadly applicable to other microbiome studies.
In vitro protective activity of South Australian marine sponge and macroalgae extracts against amyloid beta (Aβ 1–42) induced neurotoxicity in PC-12 cells
Publisher: Elsevier BV
Date: 07-2018
DOI: 10.1016/J.NTT.2018.05.002
Abstract: South Australia is a bio ersity hotspot of marine sponges and macroalgae. This study aimed to evaluate the potential neuroprotective activity of extracts from these two marine sources by reducing the toxicity of human amyloid beta Aβ
Printable Hydrogel Arrays for Portable and High-Throughput Shear-Mediated Assays
Publisher: American Chemical Society (ACS)
Date: 20-06-2023
Process and economic feasibility for the production of functional food from the brown alga Ecklonia radiata
Publisher: Elsevier BV
Date: 2018
Potential products from the highly diverse and endemic macroalgae of Southern Australia and pathways for their sustainable production
Publisher: Springer Science and Business Media LLC
Date: 07-03-2013
Effect of Flammulina velutipes on the physicochemical and sensory characteristics of Cantonese sausages
Publisher: Elsevier BV
Date: 08-2019
DOI: 10.1016/J.MEATSCI.2019.04.003
Abstract: The effects of fresh and dried Flammulina velutipes (FFV and DFV) on quality and sensory characteristics of Cantonese sausages were investigated. Sausage s les were prepared by adding 0% (control), 2.5% FFV, 2.5% and 5.0% DFV, respectively, and their nutritional compositions, free amino acid profiles, lipid and protein oxidation, color and texture properties were determined. Addition of F. velutipes significantly decreased fat content while increased free amino acid contents of Cantonese sausages. Total free amino acid contents of 2.5% FFV, 2.5% DFV and 5.0% DFV incorporation were 2.8-, 2.4- and 3.5-fold as compared to control, respectively. Lipid and protein oxidation of Cantonese sausages were effectively inhibited by the addition of F. velutipes. Both FFV and DFV addition decreased hardness and chewiness while showed different effect on yellowness of s les. DFV added at 2.5% exhibited the best overall sensory acceptance. Therefore, appropriate addition of F. velutipes may be an effective way to improve meat product quality and function.
Characterisation of the shrinkage of calcium alginate gel membrane with immobilised Lactobacillus rhamnosus
Publisher: Springer Science and Business Media LLC
Date: 14-07-2000
Abstract: To quantify the shrinkage of calcium alginate gel membrane as a support matrix for immobilising cells during the fermentation of Lactobacillus rhamnosus, factors including time, pH, membrane thickness, and the concentrations of immobilised cells, lactic acid, glucose, and calcium chloride were examined by statistical experimental design. A Plackett-Burman design was used for the first screening experiment to identify the important factors which caused the ergent effects. Uniform Design, a powerful modelling design technique, was thus chosen to design the modelling experiments. Through a non-linear step-wise regression analysis, the predictive mathematical model of the shrinkage in membrane thickness was established and the significant main effects and two-factor interactions were identified. However, no significant model equations could be obtained for the shrinkage in area and volume of gel membranes. The methodology developed can be extrapolated to the quantitative characterisation of shrinkage in other immobilised gel matrices, which will be very useful in mathematical modelling, design, operation and scale-up of gel immobilised cell systems.
Commercial cultivation, industrial application, and potential halocarbon biosynthesis pathway of Asparagopsis sp.
Publisher: Elsevier BV
Date: 06-2021
Cultivation of marine sponges
Publisher: Springer Science and Business Media LLC
Date: 06-2005
DOI: 10.1007/BF02894238
New antimalarial norterpene cyclic peroxides from Xisha Islands sponge Diacarnus megaspinorhabdosa
Publisher: Elsevier BV
Date: 04-2016
DOI: 10.1016/J.BMCL.2016.02.070
Abstract: Four new norterpene cyclic peroxides (1-4), together with three known norterpene cyclic peroxides were isolated from the Xisha Islands Sponge Diacarnus megaspinorhabdosa. Their structures were elucidated on the basis of spectroscopic analyses and comparison with the related model compounds. The compounds (1-7) were evaluated for the inhibitory activity against the malaria parasite Plasmodium falciparum, all of them showed significant antimalarial activity with IC50 values in the range of 1.6-8.6 μM.
4D printing of edible insect snacks
Publisher: Elsevier BV
Date: 10-2023
Polysaccharide and phlorotannin-enriched extracts of the brown seaweed Ecklonia radiata influence human gut microbiota and fermentation in vitro
Publisher: Springer Science and Business Media LLC
Date: 27-04-2017
Vortex fluidic-mediated fabrication of fast gelated silica hydrogels with embedded laccase nanoflowers for real-time biosensing under flow
Publisher: American Chemical Society (ACS)
Date: 05-11-2020
Transforming Spirulina maxima Biomass into Ultrathin Bioactive Coatings Using an Atmospheric Plasma Jet: A New Approach to Healing of Infected Wounds
Publisher: Wiley
Date: 15-09-2023
Cellular Localization of Debromohymenialdisine and Hymenialdisine in the Marine Sponge Axinella sp. Using a Newly Developed Cell Purification Protocol
Publisher: Springer Science and Business Media LLC
Date: 19-01-2011
DOI: 10.1007/S10126-010-9347-2
Abstract: Sponges (Porifera), as the best known source of bioactive marine natural products in metazoans, play a significant role in marine drug discovery and development. As sessile filter-feeding animals, a considerable portion of the sponge biomass can be made of endosymbiotic and associated microorganisms. Understanding the cellular origin of targeted bioactive compounds from sponges is therefore important not only for providing chemotaxonomic information but also for defining the bioactive production strategy in terms of sponge aquaculture, cell culture, or fermentation of associated bacteria. The two alkaloids debromohymenialdisine (DBH) and hymenialdisine (HD), which are cyclin-dependent kinase inhibitors with pharmacological activities for treating osteoarthritis and Alzheimer's disease, have been isolated from the sponge Axinella sp. In this study, the cellular localization of these two alkaloids was determined through the quantification of these alkaloids in different cell fractions by high-performance liquid chromatography (HPLC). First, using a differential centrifugation method, the dissociated cells were separated into different groups according to their sizes. The two bioactive alkaloids were mainly found in sponge cells obtained from low-speed centrifugation. Further cell purifications were accomplished by a newly developed multi-step protocol. Four enriched cell fractions (C1, C2, C3, and C4) were obtained and subjected to light and transmission electron microscopy, cytochemical staining, and HPLC quantification. Compared to the low concentrations in other cell fractions, DBH and HD accounted for 10.9% and 6.1%, respectively, of dry weight in the C1 fraction. Using the morphological characteristics and cytochemical staining results, cells in the C1 fraction were speculated to be spherulous cells. This result shows that DBH and HD in Axinella sp. are located in sponge cells and mostly stored in spherulous cells.
Process optimisation and physicochemical characterisation of enzymatic hydrolysates of proteins from co-products of Atlantic Salmon (Salmo salar) and Yellowtail Kingfish (Seriola lalandi)
Publisher: Wiley
Date: 08-08-2012
Gut microbiome modulation and gastrointestinal digestibility in vitro of polysaccharide-enriched extracts and seaweeds from Ulva rigida and Gracilaria fisheri
Publisher: Elsevier BV
Date: 09-2022
An efficient protein isolation process for use in Limnospira maxima: A biorefinery approach
Publisher: Elsevier BV
Date: 12-2021
A phlorotannin isolated from Ecklonia radiata, Dibenzodioxin-fucodiphloroethol, inhibits neurotoxicity and aggregation of β-amyloid
Publisher: Elsevier BV
Date: 11-2021
Enhancing starch production of a marine green microalga Tetraselmis subcordiformis through nutrient limitation
Publisher: Elsevier BV
Date: 08-2012
DOI: 10.1016/J.BIORTECH.2012.05.030
Abstract: Microalgal starch is a potential feedstock for biofuel production. The effects of KNO(3) and MgSO(4) concentrations and light intensity on biomass and starch production by the marine microalga, Tetraselmis subcordiformis, were investigated. Under 200 μmol m(-2) s(-1) irradiance and sulfur-deprived conditions, a starch productivity of 0.62 g L(-1) d(-1) and a starch content of 62.1% based on dry weight (DW) was achieved. A starch content of 54.3% was achieved under low irradiance and nitrogen starvation, which was 6.5% higher than that under nutrient- and light-sufficient conditions. Photosynthetic activity was indispensable for starch accumulation. It is difficult to reach high starch productivity and starch concentration simultaneously. Proper nutrient concentrations are necessary to achieve high starch productivity or starch concentration based on the target. The high starch productivity and starch content suggest that T. subcordiformis is a promising microalgal starch producer.
Coastal Biotechnology: Facing the Global and the Regional Changes
Publisher: Hindawi Limited
Date: 2014
DOI: 10.1155/2014/191480
Laser-Ablated Vortex Fluidic-Mediated Synthesis of Superparamagnetic Magnetite Nanoparticles in Water Under Flow
Publisher: American Chemical Society (ACS)
Date: 14-09-2018
High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro
Publisher: MDPI AG
Date: 11-06-2023
DOI: 10.3390/MD21060358
Abstract: Shell wastes pose environmental and financial burdens to the shellfish industry. Utilizing these undervalued shells for commercial chitin production could minimize their adverse impacts while maximizing economic value. Shell chitin conventionally produced through harsh chemical processes is environmentally unfriendly and infeasible for recovering compatible proteins and minerals for value-added products. However, we recently developed a microwave-intensified biorefinery that efficiently produced chitin, proteins eptides, and minerals from lobster shells. Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products. This has suggested a further investigation of lobster minerals for commercial applications. In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells. The calcium from the lobster minerals was found to be comparable to that of a commercial calcium supplement (CCS, 139 vs. 148 mg/g). In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g). Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%). Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis. However, it had significant effects on cell growth and proliferation. The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT). The cell growth reached 49.9–61.6% for the MG-63 and 42.9–53.4% for the HaCaT. Furthermore, the MG-63 and HaCaT cells proliferated considerably after seven days of incubation, reaching 100.3% for MG-63 and 115.9% for HaCaT with a lobster mineral supplementation of 15%. Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24–2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold ( %). All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
Marine Actinomycetes-derived Natural Products
Publisher: Bentham Science Publishers Ltd.
Date: 03-01-2020
DOI: 10.2174/1568026619666191114102359
Abstract: Actinomycetes is an abundant resource for discovering a large number of lead compounds, which play an important role in microbial drug discovery. Compared to terrestrial microorganisms, marine actinomycetes have unique metabolic pathways because of their special living environment, which has the potential to produce a variety of bioactive substances. In this paper, secondary metabolites isolated from marine actinomycetes are reviewed (2013-2018), most of which exhibited cytotoxic, antibacterial, and antiviral biological activities.
Effect of Homogeneity on Cell Growth and Anthocyanin Biosynthesis in Suspension Cultures of Vitis vinifera
Publisher: Elsevier BV
Date: 09-2006
DOI: 10.1016/S1872-2075(06)60057-5
Abstract: The instability of secondary metabolite production is a ubiquitous problem in plant cell culture. To understand the instability, the investigation of anthocyanin accumulation in suspension cultures of Vitis vinifera, as a model system, has been initiated in our laboratory. Suspension culture of a relatively homogeneous cell line E of V. vinifera, was established by long-term cell line selection by anthocyanin content differentiation. The aggregate size of E was smaller than that of other cell lines obtained by routine screening method. The variation coefficients of anthocyanin content in suspension cultures of E were 8.7% in long-term subcultures and 5% in repeated flasks, respectively. The effects of elicitor, precursor feeding and light irridiation on biomass and anthocyanin accumulation in suspension cultures of E had been investigated and the results showed that all the variation coefficients were lower than 12% and this indicated the importance of homogeneity on stable production in plant cell culture. With the combination treatment of 30micromol/L phenylalanine and 218micromol/L methyl jasmonate in the dark in suspension cultures of E, the anthocyanin content and production in suspension culture of E was 5.89-fold and 4.30-fold of the controls, respectively, and all the variation coefficients of biomass and anthocyanin accumulation were lower than those of the controls in 5 successive subcultures.
Microalgae biofuels: can we achieve net energy gain and water savings?
Publisher: Informa UK Limited
Date: 2012
DOI: 10.4155/BFS.11.152
Larval release and settlement of the marine sponge Hymeniacidon perlevis (Porifera, Demospongiae) under controlled laboratory conditions
Publisher: Elsevier BV
Date: 05-2009
Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis
Publisher: Springer Science and Business Media LLC
Date: 07-01-2011
Neuroprotective Effect of a New Free Radical Scavenger HL-008 in an Ischemia–Reperfusion Injury Rat Model
Publisher: Elsevier BV
Date: 06-2021
Dysiherbols A-C and Dysideanone E, Cytotoxic and NF-κB Inhibitory Tetracyclic Meroterpenes from a Dysidea sp. Marine Sponge
Publisher: American Chemical Society (ACS)
Date: 10-02-2016
DOI: 10.1021/ACS.JNATPROD.5B01079
Abstract: Four new tetracyclic meroterpnes, dysiherbols A-C (1-3) and dysideanone E (4), were isolated from a Dysidea sp. marine sponge collected from the South China Sea. Their complete structures and absolute configurations were unambiguously determined by a combination of NMR spectroscopic data, ECD calculations, and single-crystal X-ray diffraction analysis. Within the sesquiterpene quinol structures, dysiherbols A-C possess an intriguing 6/6/5/6-fused tetracyclic carbon skeleton. The NF-κB inhibitory and cytotoxic activity evaluation disclosed that dysiherbol A (1) showed potent activity with respective IC50 values of 0.49 and 0.58 μM, which were about 10-fold and 20-fold more potent than those of dysiherbols B (2) and C (3), which feature hydroxy and ketone carbonyl groups at the C-3 position.
Biotechnology for the sustainability of human society
Publisher: Elsevier BV
Date: 10-2008
Abietane diterpenoids synthesized by suspension-cultured cells of Cephalotaxus fortunei
Publisher: Elsevier BV
Date: 03-2011
Development of a multilocus-based approach for sponge (phylum Porifera) identification: refinement and limitations
Publisher: Springer Science and Business Media LLC
Date: 02-02-2017
DOI: 10.1038/SREP41422
Abstract: For sponges (phylum Porifera), there is no reliable molecular protocol available for species identification. To address this gap, we developed a multilocus-based Sponge Identification Protocol (SIP) validated by a s le of 37 sponge species belonging to 10 orders from South Australia. The universal barcode COI mtDNA, 28S rRNA gene (D3–D5), and the nuclear ITS1-5.8S-ITS2 region were evaluated for their suitability and capacity for sponge identification. The highest Bit Score was applied to infer the identity. The reliability of SIP was validated by phylogenetic analysis. The 28S rRNA gene and COI mtDNA performed better than the ITS region in classifying sponges at various taxonomic levels. A major limitation is that the databases are not well populated and possess low ersity, making it difficult to conduct the molecular identification protocol. The identification is also impacted by the accuracy of the morphological classification of the sponges whose sequences have been submitted to the database. Re-examination of the morphological identification further demonstrated and improved the reliability of sponge identification by SIP. Integrated with morphological identification, the multilocus-based SIP offers an improved protocol for more reliable and effective sponge identification, by coupling the accuracy of different DNA markers.
The development of seaweed-derived bioactive compounds for use as prebiotics and nutraceuticals using enzyme technologies
Publisher: Elsevier BV
Date: 12-2017
Direct superassemblies of freestanding metal-carbon frameworks featuring reversible crystalline-phase transformation for electrochemical sodium storage
Publisher: American Chemical Society (ACS)
Date: 12-12-2016
DOI: 10.1021/JACS.6B10782
Abstract: High-power sodium-ion batteries (SIBs) with long-term cycling attract increasing attention for large-scale energy storage. However, traditional SIBs toward practical applications still suffer from low rate capability and poor cycle induced by pulverization and amorphorization of anodes at high rate (over 5 C) during the fast ion insertion/extraction process. The present work demonstrates a robust strategy for a variety of (Sb-C, Bi-C, Sn-C, Ge-C, Sb-Bi-C) freestanding metal-carbon framework thin films via a space-confined superassembly (SCSA) strategy. The sodium-ion battery employing the Sb-C framework exhibits an unprecedented performance with a high specific capacity of 246 mAh g
Manipulating anthocyanin composition in Vitis vinifera suspension cultures by elicitation with jasmonic acid and light irradiation
Publisher: Springer Science and Business Media LLC
Date: 2003
Abstract: Jasmonic acid altered the accumulation of major anthocyanins in Vitis vinifera cell culture. Peonidin 3-glucoside content at day three was increased from 0.3 to 1.7 mg g(-1) dry cell wt while other major anthocyanins were increased by smaller increments. By day 14, the content of methylated and acylated anthocyanins (peonidin 3-p-coumaroylglucoside and malvidin 3-p-coumaroylglucoside) was 6.3 mg g(-1) DCW, in response to treatment with jasmonic acid, and comprising approximately 45% (w/w) of total anthocyanins. In comparison, the untreated control culture contained 1.2 mg g(-1) DCW which made up approximately 32% (w/w) of total anthocyanins. Light further enhanced anthocyanin accumulation induced by jasmonic acid elicitation. The content of peonidin 3-glucoside at day 3 was 6.6 mg g(-1) DCW, 22-fold higher than control cultures while the content in response to light irradiation alone was 0.6 mg g(-1) DCW. When a highly pigmented cell line was elicited with jasmonic acid total anthocyanins increased from 9.2 to 20.7 mg g(-1) DCW, but there was no change in the anthocyanin composition.
Techno-economic feasibility analysis of microwave-assisted biorefinery of multiple products from Australian lobster shells
Publisher: Elsevier BV
Date: 11-2020
Protein hydrolysates produced from rock lobster (Jasus edwardsii) Head: emulsifying capacity and food safety
Publisher: Wiley
Date: 10-03-2016
DOI: 10.1002/FSN3.352
Related Organisations
Japan Society For The Promotion Of Science
Location: No location found
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