ORCID Profile
0000-0001-9173-1180
Current Organisations
University of Sheffield
,
Lancaster University
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Research Topics
In Research Link Australia (RLA), "Research Topics" refer to ANZSRC FOR and SEO codes. These topics are either sourced from ANZSRC FOR and SEO codes listed in researchers' related grants or generated by a large language model (LLM) based on their publications.
Top 5 Research Topics
ANZSRC Field of Research (FoR)
Materials Engineering | Colloid and Surface Chemistry | Macromolecular and Materials Chemistry | Chemical Characterisation of Materials | Materials Engineering not elsewhere classified | Plasma Physics; Fusion Plasmas; Electrical Discharges | Nanotechnology | Physical Chemistry of Materials | Characterisation of Biological Macromolecules | Biomaterials | Polymerisation Mechanisms | Polymerisation Mechanisms | Physical Chemistry (Incl. Structural) | Environmental Technologies | Polymers | Manufacturing Engineering not elsewhere classified | Medical Physics | Nanomanufacturing | Functional Materials | Manufacturing Engineering | Macromolecular and Materials Chemistry not elsewhere classified | Materials Engineering Not Elsewhere Classified | Composite and Hybrid Materials | Environmental Nanotechnology
ANZSRC Socio-Economic Objective (SEO)
Expanding Knowledge in the Chemical Sciences | Expanding Knowledge in the Physical Sciences | Expanding Knowledge in Engineering | Manufacturing not elsewhere classified | Chemical sciences | Physical sciences | Expanding Knowledge in the Medical and Health Sciences | Expanding Knowledge in Technology | Water services and utilities | Health Related to Ageing | Scientific Instruments | Expanding Knowledge in the Biological Sciences | Manufactured products not elsewhere classified |
Related Links
Publications
A method for the deposition of controllable chemical gradientsThis work was supported by EPSRC Grant GR/R28560/01.
Publisher: Royal Society of Chemistry (RSC)
Date: 2003
DOI: 10.1039/B305445B
On the effect of serum on the transport of reactive oxygen species across phospholipid membranes
Publisher: American Vacuum Society
Date: 24-04-2015
DOI: 10.1116/1.4918765
Abstract: The transport of plasma generated reactive oxygen species (ROS) across a simple phospholipid membrane mimic of a (real) cell was investigated. Experiments were performed in cell culture media (Dulbecco's modified Eagle's medium, DMEM), with and without 10% serum. A (broad spectrum) ROS reporter dye, 2,7-dichlorodihydrofluorescein (DCFH), was used to detect the generation of ROS by a helium (He) plasma jet in DMEM using free DCFH and with DCFH encapsulated inside phospholipid membrane vesicles dispersed in DMEM. The authors focus on the concentration and on the relative rates (arbitrary units) for oxidation of DCFH [or the appearance of the oxidized product 2,7-dichlorofluorescein (DCF)] both in solution and within vesicles. In the first 1 h following plasma exposure, the concentration of free DCF in DMEM was ∼15× greater in the presence of serum (cf. to the serum-free DMEM control). The DCF in vesicles was ∼2× greater in DMEM containing serum compared to the serum-free DMEM control. These data show that serum enhances plasma ROS generation in DMEM. As expected, the role of the phospholipid membrane was to reduce the rate of oxidation of the encapsulated DCFH (with and without serum). And the efficiency of ROS transport into vesicles was lower in DMEM containing serum (at 4% efficiency) when compared to serum-free DMEM (at 32% efficiency). After 1 h, the rate of DCFH oxidation was found to have significantly reduced. Based upon a synthesis of these data with results from the open literature, the authors speculate on how the components of biological fluid and cellular membranes might affect the kinetics of consumption of plasma generated ROS.
Immobilized Streptavidin Gradients as Bioconjugation Platforms
Publisher: American Chemical Society (ACS)
Date: 27-01-2012
DOI: 10.1021/LA204714P
Abstract: Surface density gradients of streptavidin (SAV) were created on solid surfaces and demonstrated functionality as a bioconjugation platform. The surface density of immobilized streptavidin steadily increased in one dimension from 0 to 235 ng cm(-2) over a distance of 10 mm. The density of coupled protein was controlled by its immobilization onto a polymer surface bearing a gradient of aldehyde group density, onto which SAV was covalently linked using spontaneous imine bond formation between surface aldehyde functional groups and primary amine groups on the protein. As a control, human serum albumin was immobilized in the same manner. The gradient density of aldehyde groups was created using a method of simultaneous plasma copolymerization of ethanol and propionaldehyde. Control over the surface density of aldehyde groups was achieved by manipulating the flow rates of these vapors while moving a mask across substrates during plasma discharge. Immobilized SAV was able to bind biotinylated probes, indicating that the protein retained its functionality after being immobilized. This plasma polymerization technique conveniently allows virtually any substrate to be equipped with tunable protein gradients and provides a widely applicable method for bioconjugation to study effects arising from controllable surface densities of proteins.
Integration of microplasma and microfluidic technologies for localised microchannel surface modification
Publisher: SPIE
Date: 21-12-2011
DOI: 10.1117/12.903293
Role of Positive Ions in Determining the Deposition Rate and Film Chemistry of Continuous Wave Hexamethyl Disiloxane Plasmas
Publisher: American Chemical Society (ACS)
Date: 06-09-2011
DOI: 10.1021/LA202010N
Abstract: New data shed light on the mechanisms of film growth from low power, low pressure plasmas of organic compounds. These data rebalance the widely held view that plasma polymer formation is due to radical/neutral reactions only and that ions play no direct role in contributing mass at the surface. Ion reactions are shown to play an important role in both the plasma phase and at the surface. The mass deposition rate and ion flux in continuous wave hexamethyl disiloxane (HMDSO) plasmas have been studied as a function of pressure and applied RF power. Both the deposition rate and ion flux were shown to increase with applied power however, the deposition rate increased with pressure while the ion flux decreased. Positive ion mass spectrometry of the plasma phase demonstrates that the dominant ionic species is the (HMDSO-CH(3))(+) ion at m/z 147, but significant fragmentation and subsequent oligomerization was also observed. Chemical analysis of the deposits by X-ray photoelectron spectroscopy and secondary ion mass spectrometry show that the deposits were consistent with deposits reported by previous workers grown from plasma and hyperthermal (HMDSO-CH(3))(+) ions. Increasing coordination of silicon with oxygen in the plasma deposits reveals the role of ions in the growth of plasma polymers. Comparing the calculated film thicknesses after a fixed total fluence of 1.5 × 10(19) ions/m(2) to results for hyperthermal ions shows that ions can contribute significantly to the total absorbed mass in the deposits.
A substrate independent approach for generation of surface gradients
Publisher: Elsevier BV
Date: 2013
Creating gradients of two proteins by differential passive adsorption onto a PEG-density gradient
Publisher: Elsevier BV
Date: 2010
DOI: 10.1016/J.BIOMATERIALS.2009.09.056
Abstract: Many fundamental biological processes, including early embryo development, immune responses and the progression of pathogens, are mediated by gradients of biological molecules. Understanding these vital physiological processes requires the development of biomaterial platforms that can mimic them in-vitro. Such platforms include laboratory generated surface gradients of biological molecules. In this work, we report a method for the generation of surface gradients of two proteins. We used a surface grafting density gradient of polyethylene glycol (PEG) to control protein adsorption. In addition, we used protein size as a tool to control the position and the adsorbed amount of both proteins. To demonstrate our concept, we used fibrinogen as an ex le of a large protein and lysozyme as an ex le of a small protein. However, we speculate that the same strategy could be extended to any other pair of large and small proteins. We used X-ray photoelectron spectroscopy and sessile drop contact angle measurements to determine the chemical composition and wettability of the gradients. Protein adsorption was studied by surface plasmon resonance imaging.
Reply to “Testing the Hypothesis: Comments on Plasma Polymerization of Acrylic Acid Revisited”
Publisher: Wiley
Date: 08-06-2011
Application of Variable-Temperature Ellipsometry to Plasma Polymers: The Effect of Addition of 1,7-Octadiene to Plasma Deposits of Acrylic Acid
Publisher: American Chemical Society (ACS)
Date: 08-03-2000
DOI: 10.1021/CM9907413
Differences in the Aging of Allyl Alcohol, Acrylic Acid, Allylamine, and Octa-1,7-diene Plasma Polymers As Studied by X-ray Photoelectron Spectroscopy
Publisher: American Chemical Society (ACS)
Date: 12-08-2000
DOI: 10.1021/CM0002158
On the effects of atmospheric-pressure microplasma array treatment on polymer and biological materials
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C3RA42025F
UV–vis spectroscopy study of plasma-activated water: Dependence of the chemical composition on plasma exposure time and treatment distance
Publisher: IOP Publishing
Date: 13-11-2017
The use of a micro-cavity discharge array at atmospheric pressure to investigate the spatial modification of polymer surfaces
Publisher: Elsevier BV
Date: 04-2010
Surface Morphology in the Early Stages of Plasma Polymer Film Growth from Amine‐Containing Monomers
Publisher: Wiley
Date: 15-03-2011
A Proposal for Enhanced Supervision of the Refugee Convention
Publisher: Oxford University Press (OUP)
Date: 09-2013
DOI: 10.1093/JRS/FET021
Development of a microtiter plate-based glycosaminoglycan array for the investigation of glycosaminoglycan-protein interactions
Publisher: Oxford University Press (OUP)
Date: 03-09-2009
Rational approaches for optimizing chemical functionality of plasma polymers: A case study with ethyl trimethylacetate
Publisher: Wiley
Date: 07-12-2020
Surface protein gradients generated in sealed microchannels using spatially varying helium microplasma
Publisher: AIP Publishing
Date: 2015
DOI: 10.1063/1.4913367
Abstract: Spatially varied surface treatment of a fluorescently labeled Bovine Serum Albumin (BSA) protein, on the walls of a closed (sealed) microchannel is achieved via a well-defined gradient in plasma intensity. The microchips comprised a microchannel positioned in-between two microelectrodes (embedded in the chip) with a variable electrode separation along the length of the channel. The channel and electrodes were 50 μm and 100 μm wide, respectively, 50 μm deep, and adjacent to the channel for a length of 18 mm. The electrode separation distance was varied linearly from 50 μm at one end of the channel to a maximum distance of 150, 300, 500, or 1000 μm to generate a gradient in helium plasma intensity. Plasma ignition was achieved at a helium flow rate of 2.5 ml/min, 8.5 kVpk-pk, and 10 kHz. It is shown that the plasma intensity decreases with increasing electrode separation and is directly related to the residual amount of BSA left after the treatment. The plasma intensity and surface protein gradient, for the different electrode gradients studied, collapse onto master curves when plotted against electrode separation. This precise spatial control is expected to enable the surface protein gradient to be tuned for a range of applications, including high-throughput screening and cell-biomolecule-biomaterial interactions.
Microplasma jet treatment of bovine serum albumin coatings for controlling enzyme and cell attachment
Publisher: Springer Science and Business Media LLC
Date: 08-2017
In-situ QCM-D analysis reveals four distinct stages during vapour phase polymerisation of PEDOT thin films
Publisher: Elsevier BV
Date: 04-2010
Method for the Generation of Surface-Bound Nanoparticle Density Gradients
Publisher: American Chemical Society (ACS)
Date: 08-02-2011
DOI: 10.1021/JP111221G
Modelling the helium plasma jet delivery of reactive species into a 3D cancer tumour
Publisher: IOP Publishing
Date: 08-12-2017
Chemical and thermo-responsive characterisation of surfaces formed by plasma polymerisation ofN-isopropyl acrylamide
Publisher: Wiley
Date: 2006
DOI: 10.1002/SIA.2318
Combined effect of protein and oxygen on reactive oxygen and nitrogen species in the plasma treatment of tissue
Publisher: AIP Publishing
Date: 07-09-2015
DOI: 10.1063/1.4930874
Abstract: The influence of protein and molecular, ground state oxygen (O2) on the plasma generation, and transport of reactive oxygen and nitrogen species (RONS) in tissue are investigated. A tissue target, comprising a 1 mm thick gelatin film (a surrogate for real tissue), is placed on top of a 96-well plate each well is filled with phosphate buffered saline (PBS, pH 7.4) containing one fluorescent or colorimetric reporter that is specific for one of three RONS (i.e., H2O2, NO2−, or OH•) or a broad spectrum reactive oxygen species reporter (2,7-dichlorodihydrofluorescein). A helium cold atmospheric plasma (CAP) jet contacts the top of the gelatin surface, and the concentrations of RONS generated in PBS are measured on a microplate reader. The data show that H2O2, NO2−, or OH• are generated in PBS underneath the target. Independently, measurements are made of the O2 concentration in the PBS with and without the gelatin target. Adding bovine serum albumin protein to the PBS or gelatin shows that protein either raises or inhibits RONS depending upon the O2 concentration. Our results are discussed in the context of plasma-soft tissue interactions that are important in the development of CAP technology for medicine, biology, and food manufacturing.
Approaches to Quantify Amine Groups in the Presence of Hydroxyl Functional Groups in Plasma Polymerized Thin Films
Publisher: Wiley
Date: 11-07-2014
Plasma Parameter Aspects in the Fabrication of Stable Amine Functionalized Plasma Polymer Films
Publisher: Wiley
Date: 12-02-2015
Modulating the concentrations of reactive oxygen and nitrogen species and oxygen in water with helium and argon gas and plasma jets
Publisher: IOP Publishing
Date: 23-11-2018
Haptotatic Plasma Polymerized Surfaces for Rapid Tissue Regeneration and Wound Healing
Publisher: American Chemical Society (ACS)
Date: 22-11-2016
Abstract: Skin has a remarkable capacity for regeneration however, with an ever aging population, there is a growing burden to the healthcare system from chronic wounds. Novel therapies are required to address the problems associated with nonhealing chronic wounds. Novel wound dressings that can encourage increased reepithelialization could help to reduce the burden of chronic wounds. A suite of chemically defined surfaces have been produced using plasma polymerization, and the ability of these surfaces to support the growth of primary human skin cells has been assessed. Additionally, the ability of these surfaces to modulate cell migration and morphology has also been investigated. Keratinocytes and endothelial cells were extremely sensitive to surface chemistry showing increased viability and migration with an increased number of carboxylic acid functional groups. Fibroblasts proved to be more tolerant to changes in surface chemistry however, these cells migrated fastest over amine-functionalized surfaces. The novel combination of comprehensive chemical characterization coupled with the focus on cell migration provides a unique insight into how a material's physicochemical properties affect cell migration.
Electrical and optical properties of a gradient microplasma for microfluidic chips
Publisher: Wiley
Date: 06-01-2017
Adsorption of immunoglobulin G to plasma-co-polymer surfaces of acrylic acid and 1,7-octadiene
Publisher: Royal Society of Chemistry (RSC)
Date: 2003
DOI: 10.1039/B302176A
Development of a surface to enhance the effectiveness of fibroblast growth factor 2 (FGF-2)
Publisher: Royal Society of Chemistry (RSC)
Date: 2014
DOI: 10.1039/C4BM00018H
Abstract: The cooperative effects of FGF-2, surface bound heparin and plasma polymer, produce a biologically active surface which increases the half-life of FGF-2. The production of this surface is simple and requires no chemical cross-linking.
Genotoxicity and cytotoxicity of the plasma jet-treated medium on lymphoblastoid WIL2-NS cell line using the cytokinesis block micronucleus cytome assay
Publisher: Springer Science and Business Media LLC
Date: 20-06-2017
DOI: 10.1038/S41598-017-03754-1
Abstract: Despite growing interest in the application of atmospheric plasma jets as medical treatment strategies, there has been comparatively little research on the potential genotoxic and cytotoxic effects of plasma jet treatment. In this study, we have employed the cytokinesis block micronucleus cytome (CBMN-Cyt) assay with WIL2-NS B lymphoblastoid cells to test the potential genotoxicity, as well as the cytotoxicity, of toxic species generated in cell culture media by an argon (Ar) plasma jet. Elevated levels of cell death (necrosis) and occurrence of chromosomal damage (micronuclei MN, nculeoplasmic bridge NPBs and nuclear bus, Nbuds) were observed when cells were exposed to plasma jet-treated media. These results provide a first insight into how we might measure the genotoxic and cytotoxic effect of plasma jet treatments (both indirect and direct) in iding human cells.
Mass spectral investigation of the plasma phase of a pulsed plasma of acrylic acid
Publisher: American Vacuum Society
Date: 11-2000
DOI: 10.1116/1.1316099
Development of a surface to increase retinal pigment epithelial cell (ARPE-19) proliferation under reduced serum conditions
Publisher: Springer Science and Business Media LLC
Date: 04-02-2014
DOI: 10.1007/S10856-014-5163-Y
Abstract: Age related macular degeneration of the eye is brought about by damage to the retinal pigment epithelium (RPE) and is a major cause of adult blindness. One potential treatment method is transplantation of RPE cells grown in vitro. Maintaining RPE cell viability and physiological function in vitro is a challenge, and this must also be achieved using materials that can be subsequently used to deliver an intact cell sheet into the eye. In this paper, plasma polymerisation has been used to develop a chemically modified surface for maintaining RPE cells in vitro. Multiwell plates modified with a plasma copolymer of allylamine and octadiene maintained RPE cell growth at a level similar to that of TCPS. However, the addition of bound glycosaminoglycans (GAGs) to the plasma polymerised surface significantly enhanced RPE proliferation. Simply adding GAG to the culture media had no positive effect. It is shown that a combination of plasma polymer and GAG is a promising method for developing suitable surfaces for cell growth and delivery, that can be applied to any substrate material.
Early Stages of Growth of Plasma Polymer Coatings Deposited from Nitrogen‐ and Oxygen‐Containing Monomers
Publisher: Wiley
Date: 16-07-2010
Abstract: Ultrathin functional coatings deposited by plasma polymerization have utility in nano‐ and microtechnologies, however, until now very little has been reported to validate the widely held view that these coatings can be deposited onto any type of substrate, without substrate influence. In order to ascertain the role of the substrate in the early stages of plasma growth we address the growth rate and chemistry of plasma polymer coatings from two nitrogen‐ and two oxygen‐containing monomers during the first stages of deposition onto gold and onto thiol MUA‐coated gold surfaces. SPR thickness measurements and XPS analyzes indicate the substrate must be taken into account when ultrathin plasma polymer coatings are used for surface modification and we speculate on why this should be so. magnified image
Tracking the Penetration of Plasma Reactive Species in Tissue Models
Publisher: Elsevier BV
Date: 06-2018
DOI: 10.1016/J.TIBTECH.2017.07.012
Abstract: Electrically generated cold atmospheric plasma is being intensively researched for novel applications in biology and medicine. Significant attention is being given to reactive oxygen and nitrogen species (RONS), initially generated upon plasma-air interactions, and subsequently delivered to biological systems. Effects of plasma exposure are observed to millimeter depths within tissue. However, the exact nature of the initial plasma-tissue interactions remains unknown, including RONS speciation and delivery depth, or how plasma-derived RONS intervene in biological processes. Herein, we focus on current research using tissue and cell models to learn more about the plasma delivery of RONS into biological environments. We argue that this research is vital in underpinning the knowledge required to realize the full potential of plasma in biology and medicine.
Probing the transport of plasma-generated RONS in an agarose target as surrogate for real tissue: dependency on time, distance and material composition
Publisher: IOP Publishing
Date: 16-04-2015
Mass Spectrometry Analysis of the Real-Time Transport of Plasma-Generated Ionic Species Through an Agarose Tissue Model Target
Publisher: Technical Association of Photopolymers, Japan
Date: 2017
Cell sheets in cell therapies
Publisher: Elsevier BV
Date: 02-2018
DOI: 10.1016/J.JCYT.2017.11.004
Abstract: This review aims to provide a broad introduction to the use of cell sheets and the role of materials in the delivery of cell sheets to patients within a clinical setting. Traditionally, cells sheets have been, and currently are, fabricated using established and accepted cell culture methods within standard formats (e.g., petri dishes) utilizing biological substrates. Synthetic surfaces provide a far more versatile system for culturing and delivering cell sheets. This has the potential to positively affect quality, and efficient, localized cell delivery has a significant impact on patient outcome and on the overall cost of goods. We highlight current applications of these advanced carriers and future applications of these surfaces and cell sheets with an emphasis both on clinical use and regulatory requirements.
Glycosaminoglycan (GAG) binding surfaces for characterizing GAG-protein interactions
Publisher: Elsevier BV
Date: 02-2012
DOI: 10.1016/J.BIOMATERIALS.2011.10.042
Abstract: Glycosaminoglycans play an important role in tissue organisation through interactions with a erse range of proteins, growth factors and other chemokines. In this report, we demonstrate the GAG-binding 'fingerprint' of two important GAG-binding proteins - osteoprotogerin and TIMP-3. The technique uses a straightforward method for attaching GAGs to assay surfaces in a non-covalent manner using plasma polymerization that leaves the adsorbed GAG able to participate in subsequent ligand binding. We show that OPG and TIMP-3 bind preferentially to different GAGs in a simple ELISA and that this binding does not correlate directly with simple GAG properties such as degree of sulfation. The methods outlined in this report can be easily applied to tissue engineering scaffolds in order to exploit the potential of surface-bound GAGs in influencing the structure of engineered tissues.
Fabrication and Characterization of a Porous Silicon Drug Delivery System with an Initiated Chemical Vapor Deposition Temperature-Responsive Coating
Publisher: American Chemical Society (ACS)
Date: 21-12-2015
DOI: 10.1021/ACS.LANGMUIR.5B03794
Abstract: This paper reports on the fabrication of a pSi-based drug delivery system, functionalized with an initiated chemical vapor deposition (iCVD) polymer film, for the sustainable and temperature-dependent delivery of drugs. The devices were prepared by loading biodegradable porous silicon (pSi) with a fluorescent anticancer drug c tothecin (CPT) and coating the surface with temperature-responsive poly(N-isopropylacrylamide-co-diethylene glycol inyl ether) (pNIPAM-co-DEGDVE) or non-stimulus-responsive poly(aminostyrene) (pAS) via iCVD. CPT released from the uncoated oxidized pSi control with a burst release fashion (∼21 nmol/(cm(2) h)), and this was almost identical at temperatures both above (37 °C) and below (25 °C) the lower critical solution temperature (LCST) of the switchable polymer used, pNIPAM-co-DEGDVE (28.5 °C). In comparison, the burst release rate from the pSi-pNIPAM-co-DEGDVE s le was substantially slower at 6.12 and 9.19 nmol/(cm(2) h) at 25 and 37 °C, respectively. The final amount of CPT released over 16 h was 10% higher at 37 °C compared to 25 °C for pSi coated with pNIPAM-co-DEGDVE (46.29% vs 35.67%), indicating that this material can be used to deliver drugs on-demand at elevated temperatures. pSi coated with pAS also displayed sustainable drug delivery profiles, but these were independent of the release temperature. These data show that sustainable and temperature-responsive delivery systems can be produced by functionalization of pSi with iCVD polymer films. Benefits of the iCVD approach include the application of the iCVD coating after drug loading without causing degradation of the drug commonly caused by exposure to factors such as solvents or high temperatures. Importantly, the iCVD process is applicable to a wide array of surfaces as the process is independent of the surface chemistry and pore size of the nanoporous matrix being coated.
Studying the cytolytic activity of gas plasma with self-signalling phospholipid vesicles dispersed within a gelatin matrix
Publisher: IOP Publishing
Date: 18-04-2013
Antibacterial surfaces by adsorptive binding of polyvinyl-sulphonate-stabilized silver nanoparticles
Publisher: IOP Publishing
Date: 30-04-2010
DOI: 10.1088/0957-4484/21/21/215102
Abstract: This paper presents a novel and facile method for the generation of efficient antibacterial coatings which can be applied to practically any type of substrate. Silver nanoparticles were stabilized with an adsorbed surface layer of polyvinyl sulphonate (PVS). This steric layer provided excellent colloidal stability, preventing aggregation over periods of months. PVS-coated silver nanoparticles were bound onto amine-containing surfaces, here produced by deposition of an allylamine plasma polymer thin film onto various substrates. SEM imaging showed no aggregation upon surface binding of the nanoparticles they were well dispersed on amine surfaces. Such nanoparticle-coated surfaces were found to be effective in preventing attachment of Staphylococcus epidermidis bacteria and also in preventing biofilm formation. Combined with the ability of plasma polymerization to apply the thin polymeric binding layer onto a wide range of materials, this method appears promising for the fabrication of a wide range of infection-resistant biomedical devices.
How to assess the plasma delivery of RONS into tissue fluid and tissue
Publisher: IOP Publishing
Date: 11-07-2016
Fabrication and Operation of a Microcavity Plasma Array Device for Microscale Surface Modification
Publisher: Wiley
Date: 05-03-2012
An Experimental and Analytical Study of an Asymmetric Capacitively Coupled Plasma Used for Plasma Polymerization
Publisher: Wiley
Date: 04-06-2014
Microplasma patterning of bonded microchannels using high-precision “injected” electrodes
Publisher: Royal Society of Chemistry (RSC)
Date: 2011
DOI: 10.1039/C0LC00339E
Abstract: A rapid, high-precision method for localised plasma-treatment of bonded PDMS microchannels is demonstrated. Patterned electrodes were prepared by injection of molten gallium into preformed microchannel guides. The electrode guides were prepared without any additional fabrication steps compared to conventional microchannel fabrication. Alignment of the "injected" electrodes is precisely controlled by the photomask design, rather than positioning accuracy of alignment tools. Surface modification is detected using a fluorescent dye (Rhodamine B), revealing a well-defined micropattern with regions less than 100 µm along the length of the microchannel.
Protein Patterning on Microplasma-Activated PEO-Like Coatings
Publisher: Wiley
Date: 14-01-2014
Defining Plasma Polymerization: New Insight Into What We Should Be Measuring
Publisher: American Chemical Society (ACS)
Date: 14-06-2013
DOI: 10.1021/AM401484B
Abstract: External parameters (RF power and precursor flow rate) are typically quoted to define plasma polymerization experiments. Utilizing a parallel-plate electrode reactor with variable geometry, it is shown that these parameters cannot be transferred to reactors with different geometries in order to reproduce plasma polymer films using four precursors. Measurements of ion flux and power coupling efficiency confirm that intrinsic plasma properties vary greatly with reactor geometry at constant applied RF power. It is further demonstrated that controlling intrinsic parameters, in this case the ion flux, offers a more widely applicable method of defining plasma polymerization processes, particularly for saturated and allylic precursors.
ARXPS characterisation of plasma polymerised surface chemical gradients
Publisher: Wiley
Date: 18-10-2006
DOI: 10.1002/SIA.2400
Controlling the Spatial Distribution of Polymer Surface Treatment Using Atmospheric‐Pressure Microplasma Jets
Publisher: Wiley
Date: 06-12-2010
Development of Advanced Dressings for the Delivery of Progenitor Cells
Publisher: American Chemical Society (ACS)
Date: 19-01-2017
Abstract: Culture surfaces that substantially reduce the degree of cell manipulation in the delivery of cell sheets to patients are described. These surfaces support the attachment, culture, and delivery of multipotent adult progenitor cells (MAPC). It was essential that the processes of attachment/detachment to the surface did not affect cell phenotype nor the function of the cultured cells. Both acid-based and amine-based surface coatings were generated from acrylic acid, propanoic acid, diaminopropane, and heptylamine precursors, respectively. While both functional groups supported cell attachment/detachment, amine coated surfaces gave optimal performance. X-ray photoelectron spectroscopy (XPS) showed that at a primary amine to carbon surface ratio of between 0.01 and 0.02, greater than 90% of attached cells were effectively transferred to a model wound bed. A dependence on primary amine concentration has not previously been reported. After 48 h of culture on the optimized amine surface, PCR, functional, and viability assays showed that MAPC retained their stem cell phenotype, full metabolic activity, and biological function. Consequently, in a proof of concept experiment, it was shown that this amine surface when coated onto a surgical dressing provides an effective and simple technology for the delivery of MAPC to murine dorsal excisional wounds, with MAPC delivery verified histologically. By optimizing for cell delivery using a combination of in vitro and in vivo techniques, we developed an effective surface for the delivery of MAPC in a clinically relevant format.
Polymeric Material with Metal-Like Conductivity for Next Generation Organic Electronic Devices
Publisher: American Chemical Society (ACS)
Date: 08-10-2012
DOI: 10.1021/CM302899V
Cell attachment and proliferation on high conductivity PEDOT–glycol composites produced by vapour phase polymerisation
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C2BM00143H
Where physics meets chemistry: Thin film deposition from reactive plasmas
Publisher: Springer Science and Business Media LLC
Date: 12-2016
Submillimeter-Scale Surface Gradients of Immobilized Protein Ligands
Publisher: American Chemical Society (ACS)
Date: 20-03-2009
DOI: 10.1021/LA803775M
Abstract: We describe a method to produce antibody-captured ligand gradients over biologically relevant distances (hundreds of micrometers) whereby the ligand density and gradient shape may be tailored. Separation of the ligand from the solid-phase surface ensures that the biological activity of the ligand remains unaffected by immobilization. Our method involves the use of a plasma-masking method to generate a surface chemical gradient on a glass substrate to which the 9E10 antibody is covalently coupled. This antibody captures myc-tagged biomolecules. In our ex le, the antibody is then used to immobilize a gradient of the intercellular signaling molecule delta-like-1 (Dll1). To visualize the gradient of Dll1, we have used the multistep approach of binding with rabbit anti-Dll1 primary antibody and then adding colloidal-gold-conjugated secondary antibody.
How plasma induced oxidation, oxygenation, and de-oxygenation influences viability of skin cells
Publisher: AIP Publishing
Date: 14-11-2016
DOI: 10.1063/1.4967880
Abstract: The effect of oxidation, oxygenation, and de-oxygenation arising from He gas jet and He plasma jet treatments on the viability of skin cells cultured in vitro has been investigated. He gas jet treatment de-oxygenated cell culture medium in a process referred to as “sparging.” He plasma jet treatments oxidized, as well as oxygenated or de-oxygenated cell culture medium depending on the dissolved oxygen concentration at the time of treatment. He gas and plasma jets were shown to have beneficial or deleterious effects on skin cells depending on the concentration of dissolved oxygen and other oxidative molecules at the time of treatment. Different combinations of treatments with He gas and plasma jets can be used to modulate the concentrations of dissolved oxygen and other oxidative molecules to influence cell viability. This study highlights the importance of a priori knowledge of the concentration of dissolved oxygen at the time of plasma jet treatment, given the potential for significant impact on the biological or medical outcome. Monitoring and controlling the dynamic changes in dissolved oxygen is essential in order to develop effective strategies for the use of cold atmospheric plasma jets in biology and medicine.
On the Effect of Monomer Chemistry on Growth Mechanisms of Nonfouling PEG-like Plasma Polymers
Publisher: American Chemical Society (ACS)
Date: 13-02-2013
DOI: 10.1021/LA304713B
Abstract: It has been shown that both ions and neutral species may contribute to plasma polymer growth. However, the relative contribution from these mechanisms remains unclear. We present data elucidating the importance of considering monomer structure with respect to which the growth mechanism dominates for nonfouling PEG-like plasma polymers. The deposition rate for saturated monomers is directly linked with ion flux to the substrate. For unsaturated monomers, the neutral flux also plays a role, particularly at low power. Increased fragmentation of the monomer at high power reduces the ability of unsaturated monomers to grow via neutral grafting. Chemical characterization by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) confirm the role that plasma phase fragmentation plays in determining the deposition rate and surface chemistry of the deposited film. The simple experimental method used here may also be used to determine which mechanisms dominate plasma deposition for other monomers. This knowledge may enable significant improvement in future reactor design and process control.
Variability in plasma polymerization processes - An international round-robin study
Publisher: Wiley
Date: 17-06-2013
The role of UV photolysis and molecular transport in the generation of reactive species in a tissue model with a cold atmospheric pressure plasma jet
Publisher: AIP Publishing
Date: 04-03-2019
DOI: 10.1063/1.5086522
Abstract: Cold atmospheric pressure plasma jets (plasma) operated in ambient air provide a rich source of reactive oxygen and nitrogen species (RONS), which are known to influence biological processes important in disease. In the plasma treatment of diseased tissue such as subcutaneous cancer tumors, plasma RONS need to first traverse an interface between the plasma-skin surface and second be transported to millimeter depths in order to reach deep-seated diseased cells. However, the mechanisms in the plasma generation of RONS within soft tissues are not understood. In this study, we track the plasma jet delivery of RONS into a tissue model target and we delineate two processes: through target delivery of RONS generated (primarily) in the plasma jet and in situ RONS generation by UV photolysis within the target. We demonstrate that UV photolysis promotes the rapid generation of RONS in the tissue model target's surface after which the RONS are transported to millimeter depths via a slower molecular process. Our results imply that the flux of UV photons from plasma jets is important for delivering RONS through seemingly impenetrable barriers such as skin. The findings have implications not only in treatments of living tissues but also in the functionalization of soft hydrated biomaterials such as hydrogels and extracellular matrix derived tissue scaffolds.
Adsorption of vitronectin, collagen and immunoglobulin-G to plasma polymer surfaces by enzyme linked immunosorbent assay (ELISA)
Publisher: Royal Society of Chemistry (RSC)
Date: 11-07-2002
DOI: 10.1039/B201471H
Combination of iCVD and Porous Silicon for the Development of a Controlled Drug Delivery System
Publisher: American Chemical Society (ACS)
Date: 21-06-2012
DOI: 10.1021/AM300621K
Abstract: We describe a pH responsive drug delivery system which was fabricated using a novel approach to functionalize biodegradeable porous silicon (pSi) by initiated chemical vapor deposition (iCVD). The assembly involved first loading a model drug (c tothecin, CPT) into the pores of the pSi matrix followed by capping the pores with a thin pH responsive copolymer film of poly(methacrylic acid-co-ethylene dimethacrylate) (p(MAA-co-EDMA)) via iCVD. Release of CPT from uncoated pSi was identical in two buffers at pH 1.8 and pH 7.4. In contrast, the linear release rate of CPT from the pSi matrix with the p(MAA-co-EDMA) coating was dependent on the pH release of CPT was more than four times faster at pH 7.4 (13.1 nmol/(cm(2) h)) than at pH 1.8 (3.0 nmol/(cm(2) h)). The key advantage of this drug delivery approach over existing ones based on pSi is that the iCVD coating can be applied to the pSi matrix after drug loading without degradation of the drug because the process does not expose the drug to harmful solvents or high temperatures and is independent of the surface chemistry and pore size of the nanoporous matrix.
Immobilization of vitronectin‐binding heparan sulfates onto surfaces to support human pluripotent stem cells
Publisher: Wiley
Date: 20-09-2017
DOI: 10.1002/JBM.B.33999
Abstract: Functionalizing medical devices with polypeptides to enhance their performance has become important for improved clinical success. The extracellular matrix (ECM) adhesion protein vitronectin (VN) is an effective coating, although the chemistry used to attach VN often reduces its bioactivity. In vivo, VN binds the ECM in a sequence-dependent manner with heparan sulfate (HS) glycosaminoglycans. We reasoned therefore that sequence-based affinity chromatography could be used to isolate a VN-binding HS fraction (HS9) for use as a coating material to capture VN onto implant surfaces. Binding avidity and specificity of HS9 were confirmed by enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR)-based assays. Plasma polymerization of allylamine (AA) to tissue culture-treated polystyrene (TCPS) was then used to capture and present HS9 as determined by radiolabeling and ELISA. HS9-coated TCPS avidly bound VN, and this layered surface supported the robust attachment, expansion, and maintenance of human pluripotent stem cells. Compositional analysis demonstrated that 6-O- and N-sulfation, as well as lengths greater than three disaccharide units (dp6) are critical for VN binding to HS-coated surfaces. Importantly, HS9 coating reduced the threshold concentration of VN required to create an optimally bioactive surface for pluripotent stem cells. We conclude that affinity-purified heparan sugars are able to coat materials to efficiently bind adhesive factors for biomedical applications. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1887-1896, 2018.
The hormesis effect of plasma-elevated intracellular ROS on HaCaT cells
Publisher: IOP Publishing
Date: 12-11-2015
Plasma Polymer and Biomolecule Modification of 3D Scaffolds for Tissue Engineering
Publisher: Wiley
Date: 25-01-2016
Structure-directed growth of high conductivity PEDOT from liquid-like oxidant layers during vacuum vapor phase polymerization
Publisher: Royal Society of Chemistry (RSC)
Date: 2012
DOI: 10.1039/C2JM32281A
Microplasma Array Patterning of Reactive Oxygen and Nitrogen Species onto Polystyrene
Publisher: Frontiers Media SA
Date: 03-02-2017
Functionality of Proteins Bound to Plasma Polymer Surfaces
Publisher: American Chemical Society (ACS)
Date: 19-04-2012
DOI: 10.1021/AM300128N
Abstract: The deposition of a thin film layer by plasma polymerization enables the surface functionalization of a wide range of substrate materials for biointerfacial interactions. Plasma polymers can surface-bind proteins specifically via covalent linkages or nonspecifically through other irreversible adsorption mechanisms key questions are whether covalent chemisorption has indeed occurred, and whether the protein retains functionality. Here the mode of surface binding of streptavidin and the biotin binding functionality of the bound streptavidin layer are studied on plasma polymer (pp) surfaces deposited using propionaldehyde and ethanol that were plasma polymerized at different powers (P) to investigate possible mechanisms for protein binding to a range of different surface chemistries. As expected, with pp surfaces composed principally of aldehyde groups, protein conjugation appears to be specific (chemisorption) allowing the immobilization of streptavidin (SAV) molecules retaining the ability to bind biotinylated probes. To contrast with this, we present the first study of protein adsorption to ethanol pp surfaces prepared at different P. This provides an investigation into retention of the hydroxyl functionality in the pp at low P and its effect on protein adsorption. Adsorption of human serum albumin (HSA) to ethanol pp was similar to that on propionaldehyde pp except at low P (5 W) where hydroxyl group retention and hydration presumably has a role in reducing protein adsorption. Although we observed SAV adsorption to ethanol pp surfaces at all P, interestingly, the protein lost its ability to bind biotinylated probes. Thus we suggest that irreversible, nonspecific adsorption of protein on ethanol pp surfaces results in apparent protein denaturation despite the hydrophilic nature of the ethanol pp surface. We conclude by making inferences between the pp structure as measured by X-ray photoelectron spectroscopy (XPS) and the related protein adsorption mechanisms.
On-demand cold plasma activation of acetyl donors for bacteria and virus decontamination
Publisher: AIP Publishing
Date: 02-08-2021
DOI: 10.1063/5.0062787
Abstract: Antibiotics are commonly used as the first line of defense in the treatment of infectious diseases. However, the rise of antimicrobial resistance (AMR) is rendering many antibiotics less effective. Consequently, effective non-antibiotic antimicrobial strategies are urgently needed to combat AMR. This paper presents a strategy utilizing cold plasma for the “on-demand” activation of acetyl donor molecules. The process generates an aqueous-based antimicrobial formulation comprising a rich mixture of highly oxidizing molecules: peracetic acid, hydrogen peroxide, and other reactive oxygen and nitrogen species. The synergistic potent oxidative action between these molecules is shown to be highly effective at eradicating common wound pathogenic bacteria (Pseudomonas aeruginosa and Staphylococcus aureus) and at inactivating a virus (SARS-CoV-2).
Systems failure, market failure, or something else? The case of skills development in Australian innovation policy
Publisher: Informa UK Limited
Date: 23-09-2015
Nanoscale deposition of chemically functionalised films via plasma polymerisation
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C3RA41563E
Reaction-based indicator displacement assay (RIA) for the development of a triggered release system capable of biofilm inhibition
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9CC07759F
Abstract: Here, a reaction-based indicator displacement hydrogel assay (RIA) was developed for the detection of hydrogen peroxide (H 2 O 2 ) via the oxidative release of the optical reporter Alizarin Red S (ARS).
Using oxygen plasma treatment to improve the performance of electrodes for capacitive water deionization
Publisher: Elsevier BV
Date: 09-2013
How membrane lipids influence plasma delivery of reactive oxygen species into cells and subsequent DNA damage: an experimental and computational study
Publisher: Royal Society of Chemistry (RSC)
Date: 2019
DOI: 10.1039/C9CP03520F
Abstract: The combination of phospholipid vesicle experiments and molecular dynamics (MD) simulations illustrate how lipid oxidation, lipid packing and rafts formation may influence the response of healthy and diseased cell membranes to plasma-derived RONS.
In-situ UV Absorption Spectroscopy for Monitoring Transport of Plasma Reactive Species through Agarose as Surrogate for Tissue
Publisher: Technical Association of Photopolymers, Japan
Date: 2015
Ionized gas (plasma) delivery of reactive oxygen species (ROS) into artificial cells
Publisher: IOP Publishing
Date: 21-08-2014
Chemical and biomolecule patterning on 2D surfaces using atmospheric pressure microcavity plasma array devices
Publisher: SPIE
Date: 21-12-2011
DOI: 10.1117/12.903304
Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency
Publisher: Mary Ann Liebert Inc
Date: 23-01-2014
The assessment of cold atmospheric plasma treatment of DNA in synthetic models of tissue fluid, tissue and cells
Publisher: IOP Publishing
Date: 15-06-2017
Promiscuous hydrogen in polymerising plasmas
Publisher: Royal Society of Chemistry (RSC)
Date: 2018
DOI: 10.1039/C7CP08166A
Abstract: The multiple roles hydrogen plays in depositing plasmas is investigated by addition of H 2 O and D 2 O to ethyltrimethylacetate plasmas.
Surface Gradient of Functional Heparin
Publisher: Wiley
Date: 18-03-2008
The potential of small chemical functional groups for directing the differentiation of kidney stem cells
Publisher: Portland Press Ltd.
Date: 26-07-2010
DOI: 10.1042/BST0381062
Abstract: In the future, stem-cell-based therapies could offer new approaches to treat kidney disease and reduce the incidence of ESRD (end-stage renal disease), but, as yet, research in this area is only being conducted in rodents and it is not clear whether or when it could be applied to human patients. Drug therapies, on the other hand, have been very effective at delaying the progression of kidney disease, but, for various reasons, current drug regimes are not suitable for all patients. A greater understanding of the molecular mechanisms that underlie disease progression in chronic kidney disease could help to identify novel drug targets. However, progress in this area is currently hindered due to the lack of appropriate in vitro culture systems for important renal cell types, such as proximal tubule cells and podocytes. This problem could be overcome if it were possible to direct the differentiation of kidney stem cells to renal cell types in vitro. In the present review, we highlight the potential of surface gradients of small chemical functional groups to direct the differentiation of kidney stem cells.
A ‘tissue model’ to study the plasma delivery of reactive oxygen species
Publisher: IOP Publishing
Date: 27-03-2014
Slow Molecular Transport of Plasma-Generated Reactive Oxygen and Nitrogen Species and O2 through Agarose as a Surrogate for Tissue
Publisher: Begell House
Date: 2015
Design of a Microplasma Device for Spatially Localised Plasma Polymerisation
Publisher: Wiley
Date: 11-08-2011
Substrate influence on the initial growth phase of plasma-deposited polymer films
Publisher: Royal Society of Chemistry (RSC)
Date: 2009
DOI: 10.1039/B904367E
Abstract: In this communication we demonstrate that in the initial stages of deposition of ultrathin plasma polymer films, both the growth rate and the chemical composition of the films are affected by the nature of the substrate which is an important question surprisingly neglected until now.
A conical assembly of six plasma jets for biomedical applications
Publisher: AIP Publishing
Date: 22-08-2022
DOI: 10.1063/5.0104481
Abstract: A conical assembly of six plasma jets arranged in a rectangular pattern for biomedical applications is presented. The conical configuration increases the separation distance between in idual tubes within the assembly that reduces interference between in idual plasma jets and enables the jets to converge at the output, facilitating more uniform treatment as opposed to plasma jet arrays operated in parallel. Electrical and optical diagnostics of the plasma discharges and measurements of H2O2 and NO2− production in de-ionized water are used to characterize the potential suitability of the device for biomedical applications. Particularly, it was found that the efficiency in H2O2 (an important bacterial disinfection agent) production by the conical assembly of six plasma jets was more than nine-fold higher compared to its single plasma jet counterpart and that this could be achieved at a biocompatible temperature of below 300 K. Therefore, the device may find use in biomedical applications, particularly where larger area treatments are required such as for certain wounds and cancer tumors that can span areas of tens of cm2.
The Substrate and Composition Dependence of Plasma Polymer Stability
Publisher: Wiley
Date: 15-02-2010
Versatile gradients of chemistry, bound ligands and nanoparticles on alumina nanopore arrays
Publisher: IOP Publishing
Date: 14-09-2011
DOI: 10.1088/0957-4484/22/41/415601
Abstract: Nanoporous alumina (PA) arrays produced by self-ordering growth, using electrochemical anodization, have been extensively explored for potential applications based upon the unique thermal, mechanical and structural properties, and high surface-to-volume ratio of these materials. However, the potential applications and functionality of these materials may be further extended by molecular-level engineering of the surface of the pore rims. In this paper we present a method for the generation of chemical gradients on the surface of PA arrays based upon plasma co-polymerization of two monomers. We further extend these chemical gradients, which are also gradients of surface charge, to those of bound ligands and number density gradients of nanoparticles. The latter represent a highly exotic new class of materials, comprising aligned PA, capped by gold nanoparticles around the rim of the pores. Gradients of chemistry, ligands and nanoparticles generated by our method retain the porous structure of the substrate, which is important in applications that take advantage of the inherent properties of these materials. This method can be readily extended to other porous materials.
The Effect of a Silane Coupling Agent on the Hydrolytic Durability of Thin Epoxy Resin Films
Publisher: Informa UK Limited
Date: 09-2001
Reconciling the Physical and Chemical Environments of Plasma: A Commentary on “Mechanisms of Plasma Polymerisation – Reviewed from a Chemical Point of View”
Publisher: Wiley
Date: 18-06-2012
Plasma polymer chemical gradients for evaluation of surface reactivity: epoxide reaction with carboxylic acid surface groups
Publisher: Royal Society of Chemistry (RSC)
Date: 2004
DOI: 10.1039/B311256J
High conductivity PEDOT resulting from glycol/oxidant complex and glycol/polymer intercalation during vacuum vapour phase polymerisation
Publisher: Elsevier BV
Date: 04-2011
The link between mechanisms of deposition and the physico-chemical properties of plasma polymer films
Publisher: Royal Society of Chemistry (RSC)
Date: 2013
DOI: 10.1039/C3SM51039E
The Physics of Plasma Ion Chemistry: A Case Study of Plasma Polymerization of Ethyl Acetate
Publisher: American Chemical Society (ACS)
Date: 11-11-2019
DOI: 10.1021/ACS.JPCLETT.9B02855
Abstract: Deposition chemistry from plasma is highly dependent on both the chemistry of the ions arriving at surfaces and the ion energy. Typically, when measuring the energy distribution of ions arriving at surfaces from plasma, it is assumed that the distributions are the same for all ionic species. Using ethyl acetate as a representative organic precursor molecule, we have measured the ion chemistry and ion energy as a function of pressure and power. We show that at low pressure (<2 Pa) this assumption is valid however, at elevated pressures ion-molecule collisions close to the deposition surface affect both the energy and chemistry of these ions. Smaller ions are formed close to the surface and have lower energy than larger ionic species which are formed in the bulk of the plasma. The changes in plasma chemistry therefore are closely linked to the physics of the plasma-surface interface.
Related Organisations
University Of Sheffield
Location: United Kingdom of Great Britain and Northern Ireland
Lancaster University
Location: United Kingdom of Great Britain and Northern Ireland
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Amount: $260,000.00
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View Funded ActivityLinkage Infrastructure, Equipment And Facilities - Grant ID: LE140100087
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Amount: $410,000.00
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